| 1. |
- Westermark, Gunilla T., et al.
(author)
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AA-amyloidosis. Tissue component-specific association of various protein AA subspecies and evidence of a fourth SAA gene product
- 1990
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In: American Journal of Pathology. - 0002-9440. ; 137:2, s. 377-383
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Journal article (peer-reviewed)abstract
- Protein AA, the major repetitive protein subunit present in fibrils deposited in AA-amyloidosis, is an N-terminal cleavage product of a 104-amino acid precursor, serum amyloid A (SAA). Protein AA subspecies varying between 45 and 94 amino acids in length have been described. In this study it is shown that the different protein AA subspecies are not evenly distributed in amyloid deposits and that in single patients, certain subspecies of protein AA are deposited in specific tissue component sites. Thus larger protein AA subspecies occur in lower concentration in amyloid in the glomeruli compared to other sites and are especially found in amyloid in vessel walls. Three different SAA forms have been predicted from genomic and complementary DNA studies. The existence of a fourth type has been suspected from amino acid sequence studies of purified SAA. Protein AA derived from this fourth type of SAA is now shown to be present in amyloid fibrils in one of the patients studied in this paper.
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| 2. |
- Westermark, Karin, et al.
(author)
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Determination of the electronic density of states at a nanostructured TiO2/Ru-dye/electrolyte interface by means of photoelectron spectroscopy
- 2002
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In: Chemical Physics. - 0301-0104 .- 1873-4421. ; 285:1, s. 157-165
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Journal article (peer-reviewed)abstract
- In this work, photoelectron spectroscopy is used to measure the electronic density of states in the band gap region of a nanostructured TiO2 film. Such nanostructured films are used in dye-sensitized solar cells, and in this study a plain TiO2 film as well as a dye-sensitized film were examined. Further, to simulate the electrolyte effect on the energy levels at the oxide/dye/electrolyte interface in a solar cell, the density of states in the band gap region for an electrochemically prepared TiO2 film inserted with Li+ ions was measured. From the experimental findings we discuss the distribution and chemical nature of electron trap states in the band gap and the energy matching between the LUMO level of the adsorbed dye, cis-bis(4,4′-dicarboxy-2,2′-bipyridine)-bis(isothiocyanato)ruthenium(II), and the TiO2 conduction band edge.
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| 3. |
- Andersson, Thomas, et al.
(author)
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Vanlig svamp gav ovanlig hudinfektion : Fallbeskrivning
- 1999
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In: Läkartidningen. - 0023-7205 .- 1652-7518. ; :45, s. 4926-4927
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Journal article (pop. science, debate, etc.)abstract
- Vid opportunistisk svampinfektion i huden är diagnosen sällan självklar. Ett samarbete mellan dermatolog, patolog och mykolog kan behövas, som i detta fall av kutan alternarios. Denna typ av svampinfektion innefattas i begreppet feohyfomykos.
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| 4. |
- Arnander, Claes, et al.
(author)
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Three-Dimensional Technology and Bone Morphogenetic Protein in Frontal Bone Reconstruction
- 2006
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In: Journal of Craniofacial Surgery. ; 17:2, s. 275-279
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Journal article (peer-reviewed)abstract
- Osteoinductive bone morphogenetic proteins (BMPs) may be used in humans to facilitate healing of bony defects. The effect of different BMPs is, as with many other growth factors, highly dependent on the delivery vehicle. Bovine type I collagen is currently used in the clinical setting as a carrier and has been approved in several countries for human use. Here, we report the reconstruction of a frontal bone defect using heparin together with bovine type I collagen, hyaluronic acid, and fibrin as vehicles for BMP-2. A bony structure was created on the back of the patient by treating the latissimus dorsi muscle with the growth factor. A polyamide mold was used as a template to achieve the desired shape. The bone structure was transplanted into the defect site via microsurgical techniques. Although the prefabricated bone was not large enough tocover the entire frontal defect, the reconstruction was completed by using an additional cranial implant.
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| 5. |
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| 6. |
- Bondemark, Anders, 1989-, et al.
(author)
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Barriers to spurring innovation in Swedish transport infrastructure construction
- 2024
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In: Case Studies on Transport Policy. - : Elsevier. - 2213-624X .- 2213-6258. ; 15
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Journal article (peer-reviewed)abstract
- The construction industry is struggling with declining productivity. As Sweden’s largest public-sector purchaser of constructions services, the Swedish Transport Administration launched a programme aimed at increasing productivity. One important component of this programme was the introduction of design-build procurement to increase the leeway provided to contractors to identify innovative and efficient solutions. In this case study of the programme, which focuses on design-build contracts, we have conducted 27 interviews with Swedish Transport Administration project managers, contractors, and consultants to examine how successful the implementation of some of these measures has been and what hurdles they have encountered.
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| 7. |
- Chisalita, Simona Ioana, et al.
(author)
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Differential lipid profile and hormonal response in type 2 diabetes by exogenous insulin aspart versus the insulin secretagogue repaglinide, at the same glycemic control
- 2009
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In: Acta Diabetologica. - : Springer Science and Business Media LLC. - 0940-5429 .- 1432-5233. ; 46:1, s. 35-42
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Journal article (peer-reviewed)abstract
- Our aim was to study, at the same glycemic control, how treatment with either the insulin secretagogue repaglinide or exogenous insulin aspart affects endogenous insulin secretion, plasma insulin and IAPP (islet amyloid polypeptide) levels, GH-IGF (growth hormone-insulin-like growth factor) axis and plasma lipoprotein concentrations in patients with type 2 diabetes. Five patients, age 65.0 +/- A 4.1 years (mean +/- A SE), body weight 82.5 +/- A 5.0 kg, BMI (body mass index) 27.7 +/- A 1.5 kg/m(2) were treated for 10 weeks with repaglinide or insulin aspart in a randomized, cross-over study. At the end of each treatment a 24-h metabolic profile was performed. Blood glucose, C-peptide, free human insulin, free total (human and analogue) insulin, proinsulin, IAPP, IGF-I, IGFBP-1 (IGF binding protein-1), GHBP (growth hormone binding protein) and plasma lipoprotein concentrations were measured. Similar 24-h blood glucose profiles were obtained with repaglinide and insulin aspart treatment. During the repaglinide treatment, the meal related peaks of C-peptide and free human insulin were about twofold higher than during treatment with insulin aspart. Proinsulin, GHBP were higher and IAPP levels tended to be higher during repaglinide compared to insulin aspart. Postprandial plasma total cholesterol, triglycerides and apolipoprotein B concentrations were higher on repaglinide than on insulin aspart treatment. Our results show that, at the same glycemic control, treatment with exogenous insulin aspart in comparison with the insulin secretagogue repaglinide result in a lower endogenous insulin secretion, and a tendency towards a less atherogenic postprandial lipid profile.
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| 8. |
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| 9. |
- Dahlqvist, Johanna, 1979-, et al.
(author)
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Ichthyin/NIPAL4 localizes to keratins and desmosomes in epidermis and Ichthyin mutations affect epidermal lipid metabolism
- 2012
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In: Archives of Dermatological Research. - : Springer Science and Business Media LLC. - 0340-3696 .- 1432-069X. ; 304:5, s. 377-386
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Journal article (peer-reviewed)abstract
- Autosomal recessive congenital ichthyosis (ARCI) is a group of disorders characterized by abnormal desquamation of the skin and a disrupted epidermal water barrier. Ichthyin/NIPAL4 gene mutations have been identified in a subgroup of ARCI patients, but the role of ichthyin in epidermis remains elusive. In order to obtain new insights concerning the characteristics of ichthyin and the ARCI pathogenesis, we studied the expression and localization of ichthyin and related epidermal components in cultured keratinocytes and skin sections from patients with Ichthyin mutations and healthy controls. We observed an up-regulation of Ichthyin mRNA levels after in vitro differentiation of keratinocytes from both a patient with Ichthyin mutations and controls. Confocal and electron microscopy analyses of immunolabeled skin sections revealed that ichthyin localizes to desmosomes and keratins in both patients with mutant Ichthyin and controls, with an increased immunolabeling in patients. Nile red lipid analysis of skin sections exposed intra-cellular lipid accumulations in cells of the granular and cornified layers in patients but not in controls, consistent with the pathognomonic lipid membrane structures previously identified in epidermis from patients. Our combined findings indicate that ichthyin is associated with keratins and desmosomes in epidermis and is involved in lipid metabolism, possibly through processing of lamellar bodies. These results provide new clues to the understanding of the epidermal water barrier and the pathogenesis in ARCI.
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| 10. |
- Engstrand, Thomas, et al.
(author)
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A novel biodegradable delivery system for bone morphogenetic protein-2.
- 2008
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In: Plastic and reconstructive surgery. - 1529-4242. ; 121:6, s. 1920-8
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Journal article (peer-reviewed)abstract
- BACKGROUND: The efficacy of recombinant growth factors in vivo is highly dependent on the delivery vehicle. The authors investigated the osteoinductive effects of recombinant human bone morphogenetic proteins (BMP)-2 implanted together with a complex of heparin and chitosan. METHODS: Sixty rats were used. Three different carriers in gel formulation (type I collagen, heparin/type I collagen, and heparin/chitosan) were mixed with either 0, 10, or 50 microg of BMP-2, making the number of groups nine. The gels were injected into the quadriceps muscles of both legs in 45 rats (n = 10 per group). Freeze-dried formulations of the carriers were also tested with the same amounts of BMP-2 using 15 rats (n = 5 per group). Four weeks after implantation, the quality and amount of newly formed bone were assessed. RESULTS: Chitosan was shown to protect the heparinase-mediated degradation of heparin in vitro. The osteoinductive effects of BMP-2 in combination with heparin/chitosan were superior as compared with BMP-2 implanted together with type I collagen. Interestingly, the heparin/chitosan complex induced a small amount of bone also without BMP-2 added. The heparin/chitosan was completely absorbed after 4 weeks as determined by histologic evaluation, and a normal active bone formation was present. The freeze-dried formulations of the carriers demonstrated similar osteoinductive effects as the gels. CONCLUSIONS: An osteoinductive formula for clinical use is needed for general bone reconstruction. Heparin in complex with chitosan has the ability to stabilize or activate the growth factor in vivo and induce the generation of new bone in good yields.
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