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- Frommel, A. Y., et al.
(author)
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Ocean acidification has lethal and sub-lethal effects on larval development of yellowfin tuna, Thunnus albacares
- 2016
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In: Journal of Experimental Marine Biology and Ecology. - : Elsevier BV. - 0022-0981. ; 482, s. 18-24
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Journal article (peer-reviewed)abstract
- Ocean acidification (OA), the process by which increasing atmospheric CO2 is absorbed by the ocean, lowering the pH of surface waters, has been shown to affect many marine organisms negatively. It has been suggested that organisms from regions with naturally low pH waters, such as upwelling areas, could serve as models for future effects of OA and may be adapted to increased pCO(2) levels. In this study, we examined the effects of OA on yellowfin tuna, a highly pelagic species that spawns in the eastern tropical Pacific, an area that includes regions of strong upwelling events. Larvae reared at decreasing pH levels (pH 8.1, 7.6, 7.3 and 6.9) showed increasing organ damage in the kidney, liver, pancreas, eye and muscle, which correlated with decreased growth and survival. These findings complement earlier studies on organ damage in Atlantic cod and herring larvae and demonstrate that OA may have detrimental effects on fish larvae, regardless of their pre-exposure to low pH waters.
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- Wexler, J. B., et al.
(author)
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The effect of ocean acidification on otolith morphology in larvae of a tropical, epipelagic fish species, yellowfin tuna (Thunnus albacares)
- 2023
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In: Journal of Experimental Marine Biology and Ecology. - 0022-0981. ; 569
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Journal article (peer-reviewed)abstract
- Increasing ocean acidification is a concern due to its potential effects on the growth, development, and survival of early life stages of tuna in oceanic habitats and on the spatial extent of their suitable nursery habitat. To investigate the potential effects of increasing CO2 on otolith calcification of 9-day old pre-flexion stage yellowfin tuna (Thunnus albacares), an experiment was conducted at the Inter-American Tropical Tuna Commission's Achotines Laboratory in Panama during 2011. Fertilized eggs and larvae were exposed to mean pCO2 levels that ranged from present day (355 mu atm) to two levels predicted to occur in some areas of the Pacific in the near future (2013 and 3321 mu atm), and to an extreme value equivalent to long-term projections for 300 years in the future (9624 mu atm). The results indicated significantly larger otoliths (in area and perimeter) with significant, and increasing, fluctuating asymmetry at acidification levels similar to those projected for the near future and long-term. Otoliths increased significantly in size despite a significant decrease in somatic length with increasing pCO2. A consistent correlation between otolith and somatic growth of yellowfin tuna larvae among treatments was evident (i.e., larger otoliths were still associated with larger larvae within a treatment). The observed changes in otolith morphology with increasing ocean acidification have the potential to indirectly affect larval survival through dysfunction of the mechanosensory organs, but this remains to be verified in yellowfin tuna larvae.
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- Wilmes, P., et al.
(author)
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Community proteogenomics highlights microbial strain-variant protein expression within activated sludge performing enhanced biological phosphorus removal
- 2008
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In: The ISME Journal. - : Springer Science and Business Media LLC. - 1751-7362 .- 1751-7370. ; 2:8, s. 853-864
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Journal article (peer-reviewed)abstract
- Enhanced biological phosphorus removal (EBPR) selects for polyphosphate accumulating microorganisms to achieve phosphate removal from wastewater. We used high-resolution community proteomics to identify key metabolic pathways in 'Candidatus Accumulibacter phosphatis' (A. phosphatis)-mediated EBPR and to evaluate the contributions of co-existing strains within the dominant population. Overall, 702 proteins from the A. phosphatis population were identified. Results highlight the importance of denitrification, fatty acid cycling and the glyoxylate bypass in EBPR. Strong similarity in protein profiles under anaerobic and aerobic conditions was uncovered (only 3% of A. phosphatis-associated proteins exhibited statistically significant abundance differences). By comprehensive genome-wide alignment of 13,930 orthologous proteins, we uncovered substantial differences in protein abundance for enzyme variants involved in both core-metabolism and EBPR-specific pathways among the A. phosphatis population. These findings suggest an essential role for genetic diversity in maintaining the stable performance of EBPR systems and, hence, demonstrate the power of integrated cultivation-independent genomics and proteomics for the analysis of complex biotechnological systems.
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