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Träfflista för sökning "WFRF:(Winberg S) "

Search: WFRF:(Winberg S)

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  • Dimitriadou, S., et al. (author)
  • Brain monoaminergic activity during predator inspection in female Trinidadian guppies (Poecilia reticulata)
  • 2023
  • In: Behavioural Brain Research. - : Elsevier. - 0166-4328 .- 1872-7549. ; 436
  • Journal article (peer-reviewed)abstract
    • To understand the processes underpinning social decision-making, we need to determine how internal states respond to information gathered from the social environment. Brain monoamine neurotransmitters are key in the appraisal of the social environment and can reflect the internal state underlying behavioural responses to social stimuli. Here we determined the effects of conspecific partner cooperativeness during predator inspection on brain monoamine metabolic activity in Trinidadian guppies (Poecilia reticulata). We quantified the concentration of dopamine, serotonin and their metabolites across brain sections sampled immediately after ostensibly expe-riencing cooperation or defection from social partners whilst inspecting a predator model, using a familiar object as a control condition. Our results indicate dopaminergic and serotonergic activity differs with the coopera-tiveness experienced; these different neurotransmission profiles are likely to affect the expression and regulation of downstream behaviours that ultimately contribute to the patterning of cooperative interactions among in-dividuals in a population.
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  • Picelli, S, et al. (author)
  • Tn5 transposase and tagmentation procedures for massively scaled sequencing projects
  • 2014
  • In: Genome research. - : Cold Spring Harbor Laboratory. - 1549-5469 .- 1088-9051. ; 24:12, s. 2033-2040
  • Journal article (peer-reviewed)abstract
    • Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications.
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  • Result 1-10 of 68
Type of publication
journal article (60)
conference paper (4)
other publication (1)
doctoral thesis (1)
book chapter (1)
review (1)
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Type of content
peer-reviewed (57)
other academic/artistic (11)
Author/Editor
Winberg, S (19)
Winberg, G (12)
Winberg, J (10)
Winberg, Svante (7)
Normark, S (7)
Mollby, R (6)
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Soderhall, M (5)
Dahlbom, S. Josefin (4)
Bjorklund, AK (3)
Sandberg, R (3)
Thörnqvist, Per-Ove (3)
Soares, Marta C. (3)
ERNBERG, I (3)
Zabarovsky, ER (3)
Picelli, S (3)
Winberg, A. (3)
Norrlin, S (3)
Strinnholm, M (3)
Jobling, M (3)
Sagasser, S (3)
Ljungqvist, Olle, 19 ... (2)
Klein, G (2)
Lonnqvist, PA (2)
Messias, Joao P. M. (2)
Maurer, Frans (2)
Wahlestedt, C (2)
Ahlsten, G (2)
Beck, O (2)
Gizatullin, R (2)
Kashuba, VI (2)
Norin, Elisabeth (2)
Frati, L (2)
Faggioni, A (2)
Trivedi, P (2)
Cuomo, L (2)
Ernberg, Ingemar (2)
Höglund, E (2)
Faridani, OR (2)
Sjöberg, S (2)
Butler, A. (2)
Möllby, Roland (2)
Midtvedt, Tore (2)
Winberg, P (2)
Dahl, Margareta (2)
Zabarovsky, Eugene R (2)
Mullens, S. (2)
de Abreu, Murilo S (2)
Winberg, Petra (2)
Dotremont, C (2)
Kashuba, Vladimir I (2)
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University
Uppsala University (32)
Karolinska Institutet (31)
University of Gothenburg (5)
Lund University (3)
Umeå University (2)
Örebro University (2)
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Chalmers University of Technology (1)
Swedish University of Agricultural Sciences (1)
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Language
English (68)
Research subject (UKÄ/SCB)
Natural sciences (13)
Medical and Health Sciences (11)
Social Sciences (1)

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