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1.	Kaida, Rumi, et al. (author) Activation of beta-Glucan Synthases by Wall-Bound Purple Acid Phosphatase in Tobacco Cells 2009 In: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 150:4, s. 1822-1830 Journal article (peer-reviewed)abstract Wall-bound purple acid phosphatases have been shown to be potentially involved in the regulation of plant cell growth. The aim of this work was to further investigate the function of one of these phosphatases in tobacco (Nicotiana tabacum), NtPAP12, using transgenic cells overexpressing the enzyme. The transgenic cells exhibited a higher level of phosphatase activity in their walls. The corresponding protoplasts regenerating a cell wall exhibited a higher rate of beta-glucan synthesis and cellulose deposition was increased in the walls of the transgenic cells. A higher level of plasma membrane glucan synthase activities was also measured in detergent extracts of membrane fractions from the transgenic line, while no activation of Golgi-bound glycan synthases was detected. Enzymatic hydrolysis and methylation analysis were performed on the products synthesized in vitro by the plasma membrane enzymes from the wild-type and transgenic lines extracted with digitonin and incubated with radioactive UDP-glucose. The data showed that the glucans consisted of callose and cellulose and that the amount of each glucan synthesized by the enzyme preparation from the transgenic cells was significantly higher than in the case of the wildtype cells. The demonstration that callose and cellulose synthases are activated in cells overexpressing the wall-bound phosphatase NtPAP12 suggests a regulation of these carbohydrate synthases by a phosphorylation/dephosphorylation process, as well as a role of wall-bound phosphatases in the regulation of cell wall biosynthesis.
2.	Yumi Hayashi, S, et al. (author) Improvement of left ventricular synchronicity, assessed by tissue synchronization imaging, after a single hemodialysis session in chronic hemodialysis patients. 2009 Conference paper (other academic/artistic)
3.	Yumi Hayashi, S, et al. (author) The prevalence of intraventricular dyssynchrony, detected by tissue synchronization imaging, in hemodialysis, peritoneal dialysis and chronic kidney disease stages 3 and 4. 2009 Conference paper (other academic/artistic)
4.	Yumi Hayashi, S, et al. (author) The prevalence of right ventricular dysfunction and the acute effect of HD on right ventricular function in CKD 2009 Conference paper (other academic/artistic)

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Type of publication: conference paper (3); journal article (1)

Type of content: other academic/artistic (3); peer-reviewed (1)

Author/Editor: Nowak, J. (3); Larsson, M (3); Lindholm, B (3); Bjällmark, Anna (3); Seeberger, A (3); Riella, M (3); show more...; Brodin, L-Å (3); Yumi Hayashi, S (3); Mazza do Nascimento, ... (3); Lind, B (2); Bulone, Vincent (1); Kaida, Rumi (1); Satoh, Yumi (1); Yamada, Yohko (1); Kaku, Tomomi (1); Hayashi, Takahisa (1); Kaneko, Takako S. (1); show less...

University: Jönköping University (3); Royal Institute of Technology (1)

Language: English (4)

Research subject (UKÄ/SCB): Engineering and Technology (3)

Year: 2009 (4)

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