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2. |
- Ivanova, L N, et al.
(author)
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[Vasopressin : the ontogeny of antidiuretic action at the cellular level].
- 1989
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In: Fiziologicheskiĭ zhurnal SSSR imeni I. M. Sechenova. - 0015-329X. ; 75:7, s. 970-9
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Journal article (peer-reviewed)abstract
- The data on the development of molecular mechanism of the antidiuretic effect of vasopressin and the molecular structure of the AVP receptor, cytosolic cAMP-dependent protein-kinases and renal response to AVP, are discussed. The experiments were performed in normal rats and mice, nephrogenic diabetes insipidus mutants and rats treated with cortisol in early postnatal period. The development of the kidney sensitivity to AVP seems to be closely connected with the development of the molecular structure of the AVP receptor, age-related increase of the AVP-activated adenylate cyclase, and the maturation of cAMP-dependent protein kinases.
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3. |
- Solenov, E I, et al.
(author)
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[Changes in the receptor link during the development of renal sensitivity to aldosterone and antidiuretic hormone].
- 1986
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In: Fiziologicheskiĭ zhurnal SSSR imeni I. M. Sechenova. - 0015-329X. ; 72:12, s. 1673-9
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Journal article (peer-reviewed)abstract
- The aldosterone, cAMP and ADH receptors were studied in kidneys of 10-14- and 60-day old rats. Concentrations of aldosterone and cAMP receptors were reduced, while ADH binding was increased during the period of maturation of kidney functions. The investigation of the affinity and molecular weights of cAMP and ADH receptors suggests their complex nature and altering of their individual properties during ontogenesis. Aldosterone and cAMP with their receptors seem to participate in the regulation of gene activity in ontogenesis and ADH seems to participate in the formation of its own receptors.
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4. |
- Zelenina, M N, et al.
(author)
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[The functional characteristics of Gs-protein in the developing mammalian kidney].
- 1994
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In: Fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk. - 1027-3646. ; 80:7, s. 81-7
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Journal article (peer-reviewed)abstract
- Characteristics of G-proteins were studied in renal medulla of rats and mice of different ages. It was established that G-protein alpha-subunit gene expression has a specific dynamics with maximum on the 5-8-th day of life and at the end of weaning. It was shown that vasopressin does not stimulate G-protein GTPase activity in membrane preparations from immature kidneys. The differences of G-protein chromatographic characteristics were revealed. It is suggested that the development of vasopressin sensitivity of the kidney is related with the changes in G-protein system.
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5. |
- Ivanova, L N, et al.
(author)
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Decrease in the response to ADH of the rat kidney as a result of early postnatal treatment with cortisol.
- 1987
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In: Pflügers Archiv. - 0031-6768 .- 1432-2013. ; 408:4, s. 328-32
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Journal article (peer-reviewed)abstract
- Wistar rats were injected just once, intraperitoneally with cortisol (1 microgram/g) or saline at the age of 5 days. The cortisol-treated rats did not differ significantly in the (U/P)osm ratio from the saline-treated controls before 15 days of life. Their response to ADH was distinct but weaker than in the saline controls aged 30 days. This reduced response persisted to 60 days of life. In the collecting tubule fragments, (3H)AVP specific binding was lower in the cortisol-treated rats than in the controls at the age of 20 and 60 days. There was no (3H)AVP specific binding in the proximal convoluted tubules in the cortisol- and saline-injected rats of both ages. The ontogenetic patterns of cAMP specific binding in the papillary cytosolic fraction were different: the early increase in cAMP binding was protracted in the cortisol-treated rats, and no peak appeared at the age of 25 days. Cytosolic protein kinase activity was lower, no peak appeared at 30 days, no activation of protein kinase occurred to the end of weaning in the cortisol-treated rats. The difference between the cortisol and saline groups was abolished by day 30. The interference of cortisol with the ontogenetic changes in AVP binding capacity and cAMP-dependent protein kinase appears to be a plausible cause of the altered development of the response to ADH.
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6. |
- Solenov, E I, et al.
(author)
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[ADH and cAMP receptor binding in the kidney medulla of mice insensitive to ADH].
- 1991
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In: Fiziologicheskiĭ zhurnal SSSR imeni I. M. Sechenova. - 0015-329X. ; 77:5, s. 85-91
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Journal article (peer-reviewed)abstract
- Specific binding of ADH by the membrane fraction of the kidney medulla was lower in the normal CBA mice than in mutant mice with nephrogenic diabetes. Gel filtration of the solubilized ADH receptors of mutants revealed the presence of an unidentified factor which caused cooperative binding of ADH. DEAE-chromatography revealed no difference between cytosolic cAMP receptors in normal and mutant animals. Assay of GTP-ase activity of the membrane fraction revealed that ADH increased this parameter in CBA mice but not in mutant animals. Cholera toxin significantly diminished membrane ATP-ase activity whereas membrane preparations from mutant mice developed a reactivity to ADH. GTP binding ability in these preparations was higher than inn intact ones. In CBA mice this ability increased dramatically. HPLC profiles of G-protein complexes with GNP were very different in CBA and mutant mice. Mutation seems to cause changes both in binding and in "cross-talk" link op-complex membrane receptor of ADH.
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7. |
- Zelenina, A., et al.
(author)
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Silicon nanocrystals in SiNx/SiO2 hetero-superlattices : The loss of size control after thermal annealing
- 2014
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In: Journal of Applied Physics. - : AIP Publishing. - 0021-8979 .- 1089-7550. ; 115:24, s. 244304-
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Journal article (peer-reviewed)abstract
- Superlattices containing 3 nm thick silicon rich silicon nitride sublayers and 3 nm and 10 nm thick SiO2 barriers were prepared by plasma enhanced chemical vapor deposition. Despite the as-prepared samples represented a well-kept multilayer structure with smooth interfaces, the high temperature annealing resulted in the total destruction of multilayer structure in the samples containing 3 nm SiO2 barriers. Energy-filtered transmission electron microscopy images of these samples indicated a silicon nanoclusters formation with sizes of 2.5-12.5 nm, which were randomly distributed within the structure. Although in the sample with 10 nm SiO2 barriers some fragments of the multilayer structure could be still observed after thermal annealing, nevertheless, the formation of large nanocrystals with diameters up to 10 nm was confirmed by dark field transmission electron microscopy. Thus, in contrast to the previously published results, the expected size control of silicon nanocrystals was lost. According to the FTIR results, the thermal annealing of SiNx/SiO2 superlattices led to the formation of silicon nanocrystals in mostly oxynitride matrix. Annealed samples demonstrated a photoluminescence peak at 885 nm related to the luminescence of silicon nanocrystals, as confirmed by time-resolved photoluminescence measurements. The loss of nanocrystals size control is discussed in terms of the migration of oxygen atoms from the SiO2 barriers into the silicon rich silicon nitride sublayers. A thermodynamic mechanism responsible for this process is proposed. According to this mechanism, the driving force for the oxygen migration is the gain in the configuration entropy related to the relative arrangements of oxygen and nitrogen atoms.
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8. |
- Zelenina, M N, et al.
(author)
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[Activity of cAMP-dependent protein kinases and cAMP-binding proteins from rat renal cytosol upon dehydration].
- 1985
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In: Biokhimii͡a (Moscow, Russia). - 0320-9725. ; 50:3, s. 439-42
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Journal article (peer-reviewed)abstract
- The activity of cAMP-dependent protein kinases, cAMP binding and the spectrum of cAMP-binding proteins in renal papillary cytosol of intact rats and of rats kept on a water-deprived diet for 24 hours were investigated. It was found that the stimulation of protein kinases by 10(-6) M cAMP in the experimental group was significantly higher than in the control one. On DEAE-cellulose chromatography, the position of peaks of the specific cAMP binding corresponded to those of the regulatory cAMP-dependent protein kinases type I and II. Under these conditions, more than 80% of the binding activity in intact animals was localized in peak II, whereas in rats kept on a water-deprived diet over 60% of the binding activity was localized in peak I. The total binding activity of cytosol in experimental animals remained unchanged is compared to intact rats. It is suggested that in renal papilla dehydration is accompanied by the induction of synthesis of regulatory subunits of cAMP-dependent protein kinase type I.
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9. |
- Zelenina, M N, et al.
(author)
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[Changes in cAMP reception in the cytosol of the developing rat kidney].
- 1986
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In: Biokhimii͡a (Moscow, Russia). - 0320-9725. ; 51:1, s. 77-9
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Journal article (peer-reviewed)abstract
- The age-dependent changes in the cAMP-binding activity of renal papillary cytosol were studied in intact rats and in animals with experimental delay in development of the concentrating renal function induced by hydrocortisone injection in early postnatal period. The age dynamics of specific cAMP binding in the experimental group differ significantly from that in intact and control (sham injected) animals. It is suggested that the developmental changes in experimental animal kidneys are due to disturbances in hormonal regulation of renal tissue differentiation caused by the changes in intracellular receptors of cAMP. Another possible reason for the observed delay of development of the renal function is the weakening of the stimulating effect of endogenous cAMP on the genome function due to cAMP receptor deficiency in the cell.
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