| 1. |
- Wang, Pan, et al.
(author)
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Genome-wide study identifies the regulatory glycosyltransferase genes networks and signaling pathways from Keshan disease
- 2014
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In: Journal of Health Science. - : David Publishing Company. ; 2:4, s. 165-173
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Journal article (peer-reviewed)abstract
- KD (Keshan disease) is an endemic cardiomyopathy occurring only in China. Its pathogenesis is unclear till now. In the study, gene expression profiles of the PBMC (peripheral blood mononuclear cell) derived respectively from KD patients and healthy in KD areas were compared. Total RNA was isolated, amplified, labeled and hybridized to Agilent 4 × 44 K Whole Human Genome Oligonucleotide Microarray. Significant canonical pathways were analyzed by IPA (ingenuity pathway analysis) to identify differently expressed genes and pathways involved in the cardiovascular system development and function. Quantitative RT-PCR was applied tofurther validate our microarray results. Eighty-three up-regulated (ratios ≥ 2.0) and nine down-regulated glycosyltransferase genes (ratios ≤ 0.5) in PBMC in KD patients were detected by significance analysis of microarrays. Two significant canonical pathways from glycosyltransferase gene expression profiles were screened by IPA. The results of qRT-PCR show that four up-regulated (BMP1/7/10 and FGF18) and one down-regulated (BMP2) genes are consistent with those in microarray experiment, confirming the validity of the microarray data. Based on the results of the study, it is suggested that bone morphogenetic proteins and fibroblast growth factors might play an important role in the pathogenesis of KD. This further helps us to understand the pathogenesis of KD, as well as dilated cardiomyopathy
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| 2. |
- Wu, Haizhou, 1987, et al.
(author)
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Impact of lipid composition and muscle microstructure on myoglobin-mediated lipid oxidation in washed cod and pig muscle
- 2021
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In: Food Chemistry. - : Elsevier BV. - 0308-8146 .- 1873-7072. ; 336
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Journal article (peer-reviewed)abstract
- The roles of lipid oxidation substrates and muscle microstructure in lipid oxidation were investigated in two muscle models (cod and pig). Added myoglobin (Mb) promoted lipid oxidation in washed cod muscle (WCM) but not in washed pig muscle (WPM). The differing microstructure of WCM e.g. more exposed fat cells or membrane of muscle cells compared to the “denseness” or “wrapped” structure of WPM, may have contributed to the better ability of Mb to facilitate lipid oxidation in the WCM. Added phospholipids with polyenoic indexes of 282 and 24 activated Mb as an oxidant similarly in WPM while added neutral lipids and added free fatty acids had little effect. It is suggested that muscle microstructure and accessibility of Mb to phospholipids play critical roles in relation to Mb-mediated lipid oxidation while the degree of unsaturation in the phospholipids was less important.
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| 3. |
- Wu, Haizhou, 1987, et al.
(author)
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Quercetin as an inhibitor of hemoglobin-mediated lipid oxidation: Mechanisms of action and use of molecular docking
- 2022
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In: Food Chemistry. - : Elsevier BV. - 0308-8146 .- 1873-7072. ; 384
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Journal article (peer-reviewed)abstract
- The antioxidant effect of quercetin on hemoglobin(Hb)-mediated lipid oxidation and the mechanisms involved were investigated. Quercetin strongly inhibited Hb-mediated lipid oxidation in washed muscle. Quercetin showed effective hydroxyl radical scavenging ability similar to butylated hydroxytoluene (BHT). Quercetin reduced metHb resulting in formation of oxyHb. Bound quercetin decreased heme dissociation from metHb. Conversion to oxyHb and decreased heme dissociation represent routes to limit Hb-mediated lipid oxidation. Electrospray ionization mass spectrometry (ESI-MS) indicated one molecule of quercetin was covalently bound to Hb α-chain. Quercetin quinone docked 3.3 Å from the thiol of αCys(H15) but not near any other Cys residues of turkey Hb. At the docking site, hydrogen bonding between quercetin quinone and amino acids of α- and β-chain was demonstrated. This represents a path by which quercetin became covalently bound to α-chain. Molecular docking of heme proteins to polyphenols provides a template to better understand antioxidant interactions in muscle foods.
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| 4. |
- Zhang, Shulan, et al.
(author)
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Modelling the effects of mulching and fallow cropping on water balance in the Chinese Loess Plateau
- 2007
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In: Soil & Tillage Research. - : Elsevier BV. - 0167-1987 .- 1879-3444. ; 93:2, s. 283-298
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Journal article (peer-reviewed)abstract
- To improve water use efficiency on drylands it is essential to understand the mechanisms affecting water balance partitioning in arable land ecosystems. A field experiment was conducted, from October 2001 to October 2004, to evaluate the effects of field management regimes on water balance and water use efficiency in a winter wheat (Triticum aestivum L.) system in the Loess Plateau, China. The field management regimes tested were: (i) conventional agricultural management (winter wheat followed by a ploughed summer fallow); (ii) conventional management plus a fallow crop used as green manure; (iii) application of wheat straw mulch (0.8 kg m(-2)), which was retained throughout the year (no summer ploughing). A process-oriented ecosystem model (CoupModel) was calibrated with field measurements of soil water contents, leaf area indices, plant heights and crop yields, then used to generate comparative simulations of the water balance partitioning under the wheat straw mulch, fallow crop and conventional management regimes. The simulations indicated that during the experimental period mulching increased soil water storage by 5-8%, decreased soil evaporation by 11-13%, and increased wheat transpiration by 2-5% compared with the conventional management regime, thus increasing the wheat yield and water use efficiency. Furthermore, water reached deeper horizons under mulching than under conventional practice, resulting in 15% more deep percolation in a wet year. The simulation results also indicated that growing green manure during the fallow period decreased soil water storage, leading to lower wheat yields. Mulching proved to be an efficient measure for increasing yields, and possibly contributed to groundwater recharge.
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| 5. |
- Zhang, Shulan, et al.
(author)
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Soil Management Practice Effect on Water Balance of a Dryland Soil during Fallow Period on the Loess Plateau of China
- 2016
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In: Soil and Water Research. - 1801-5395. ; 11, s. 64-73
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Journal article (peer-reviewed)abstract
- To understand the mechanisms affecting water balance partitioning during fallow on drylands could improve the fallow management practices in arable land ecosystems. A three-year field experiment was conducted to evaluate the effects of field management regimes on water balance partitioning and fallow efficiency during the fallow periods under a winter wheat (Triticum aestivum L.) fallow system on the Loess Plateau, China. The fallow management regimes tested were: (i) conventional practice, (ii) catch cropping, and (iii) no tillage with wheat straw mulching. A process-oriented ecosystem model (CoupModel) was calibrated with field measurements and then used to generate comparative simulations of the water balance partitioning. The simulations indicated that mulching increased the soil water storage change by 38-71 mm during the three fallow periods, thus resulting in higher fallow efficiency by 9-12%, and decreased soil evaporation by 22-72 mm, compared with the conventional practice. Furthermore, water reached deeper horizons, resulting in 7 mm deep percolation in a wet year under mulching but not under conventional practice or catch cropping. The simulation results also showed that the catch cropping decreased the soil water storage change by 13-21 mm, although it lowered soil evaporation by 11-51 mm, and altogether reduced the fallow efficiency by 3-9%, compared to conventional practice. On the Loess Plateau of China mulching proved to be a sound measure for ensuring certain fallow efficiency and possibly benefit to the water cycle, while catch cropping negatively partitioned the water balance. The catch cropping under mulching might be another management regime to be considered.
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