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Träfflista för sökning "WFRF:(no name no name) "

Search: WFRF:(no name no name)

  • Result 1-6 of 6
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1.
  • 2019
  • Journal article (peer-reviewed)
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2.
  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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3.
  • no name, no name (author)
  • no title
  • 1900
  • Reports (other academic/artistic)
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4.
  • no name, no name (author)
  • no title
  • 2014
  • In: Molecular plant pathology. - : Springer Science and Business Media LLC. - 1464-6722 .- 1364-3703. ; 21:4, s. 519-540
  • Journal article (peer-reviewed)
  •  
5.
  • Perera, Kanishka, et al. (author)
  • A nodal solution of the scalar field equation at the second minimax level
  • 2014
  • In: Bulletin of the London Mathematical Society. - : Wiley. - 0024-6093 .- 1469-2120. ; 46, s. 1218-1225
  • Journal article (peer-reviewed)abstract
    • We prove the existence of a sign-changing eigenfunction at the second minimax level of the eigenvalue problem for the scalar field equation under a slow decay condition on the potential near infinity. The proof involves constructing a set consisting of sign-changing functions that is dual to the second minimax class. We also obtain a nonradial sign-changing eigenfunction at this level when the potential is radial.
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6.
  • Adimurthi, no first name, et al. (author)
  • On compactness in the Trudinger-Moser inequality
  • 2014
  • In: Annali della Scuola Normale Superiore di Pisa (Classe Scienze), Serie V. - 0391-173X .- 2036-2145. ; 13:2, s. 399-416
  • Journal article (peer-reviewed)abstract
    • We show that the Moser functional J(u) = integral Omega(e(4 pi u2) - 1) dx on the set B = {u is an element of H-0(1)(Omega) : parallel to del u parallel to(2) <= 1}, where Omega subset of R-2 is a bounded domain, fails to be weakly continuous only in the following exceptional case. Define g(s)w(r) = s(-1/2)w(r(s)) for s > 0. If u(k) -> u in B while lim inf J(u(k)) > J(u), then, with some s(k) -> 0, u(k) = g(sk) [(2 pi)(-1/2) min {1, log1/vertical bar x vertical bar}], up to translations and up to a remainder vanishing in the Sobolev norm. In other words, the weak continuity fails only on translations of concentrating Moser functions. The proof is based on a profile decomposition similar to that of Solimini [16], but with different concentration operators, pertinent to the two-dimensional case.
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  • Result 1-6 of 6

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