| 1. |
- Abramsson, Alexandra, 1973, et al.
(author)
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Proteomics Profiling of Single Organs from Individual Adult Zebrafish.
- 2010
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In: Zebrafish. - : Mary Ann Liebert Inc. - 1557-8542 .- 1545-8547. ; 7:2, s. 161-168
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Journal article (peer-reviewed)abstract
- Abstract The model organism zebrafish (Danio rerio) is extensively utilized in studies of developmental biology but is also being investigated in the context of a growing list of human age-related diseases. To facilitate such studies, we here present protein expression patterns of adult zebrafish organs, including blood, brain, fin, heart, intestine, liver, and skeletal muscle. Protein extracts were prepared from the different organs of two zebrafish and analyzed using liquid chromatography coupled to high-resolution tandem mass spectrometry. Zebrafish tissue was digested directly after minimal fractionation and cleaned up (the shotgun approach). Proteins were identified using Mascot software. In total, 1394 proteins were identified of which 644 were nonredundant. Of these, 373 demonstrated an organ-specific expression pattern and 57 had not been shown on protein level before. These data emphasize the need for increased research at the protein level to facilitate the selection of candidate proteins for targeted quantification and to refine systematic genetic network analysis in vertebrate development, biology, and disease.
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| 2. |
- Andersson, Marica, et al.
(author)
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Effects of Holding Density on the Welfare of Zebrafish: A Systematic Review.
- 2021
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In: Zebrafish. - : Mary Ann Liebert Inc. - 1557-8542 .- 1545-8547. ; 18:5, s. 297-306
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Journal article (peer-reviewed)abstract
- The zebrafish is becoming an increasingly popular research animal around the world. Its welfare is affected by an array of environmental factors, such as food access and water quality. Holding density is an important welfare aspect, not least due to its interaction with other housing conditions. Despite the extensive use of zebrafish in research, little is known of how densities affect its welfare. In this systematic review, we have performed a large literature search, compiled, and evaluated all publications regarding zebrafish holding density. We have analyzed how density effects growth, reproduction, and stress response, including behavior, water quality, and pathogenic outbreaks in young and adult fish. Our review shows that the holding densities tested vary largely depending on the research focus, for example, body growth or behavior. In fact, research indicates that future recommendations on holding density could depend on which welfare aspects are considered. Overall, there is a need for more studies investigating the interactive effects of density on welfare indicators, such as reproduction coupled with stress response. We stress the necessity of including holding density in universal housing guidelines and reporting information on holding conditions of larvae and adults when publishing zebrafish work.
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| 3. |
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| 4. |
- Cocco, Arianna, 1989-, et al.
(author)
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Neural expression patterns and protein modeling of the GABAA receptor ζ subunit in the adult zebrafish (Danio rerio Hamilton, 1822)
- 2018
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In: Zebrafish. - 1545-8547 .- 1557-8542.
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Journal article (peer-reviewed)abstract
- The zebrafish has a repertoire of twenty-six GABAA receptor subunits, including the paralogues π and ζ, homologous to the mammalian π. The brain mRNA levels of the π subunit are low, but the ζ gene is actively transcribed in D. rerio central nervous system and retinas. With a combined quantitative and qualitative gene expression profiling approach, this research characterized the distribution of the ζ transcript in the brain and eyes of the adult zebrafish. It was demonstrated that there are regional brain differences in the production of this transcript, with the stratum periventriculare of the tecta mesencephali having the highest mRNA levels for ζ. In the adult zebrafish retinas the bipolar cell layer presented staining for the ζ transcript. The three-dimensional structure of the ζ subunit of the GABAA receptor in zebrafish was also calculated in this study, with the human β3 as template. It presented four transmembrane α-helices and an amino-terminal β-loop framed into a disulfide bridge. It was therefore possible to place ζ in the superfamily of Cys-loop membrane proteins, to which GABAA receptor subunits belong. The current study presented a thorough characterization of a unique fish GABAA receptor subunit, which might have specific functions only typical of fish.
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| 5. |
- Gorniok, Beata Filipek, et al.
(author)
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The Nordic Zebrafish and Husbandry Meeting 2018
- 2019
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In: Zebrafish. - : MARY ANN LIEBERT, INC. - 1545-8547 .- 1557-8542. ; 16:3, s. 329-330
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Journal article (other academic/artistic)abstract
- The Nordic zebrafish and husbandry meeting took place at Karolinska Institutet in Stockholm, November 7-9, 2018. More than 120 scientists from Europe joined this meeting, which also attracted world-leading keynote speakers such as Zoltan Varga, Didier Stainier, and Hernan Lopez-Schier. The meeting comprised both scientific as well as zebrafish husbandry and animal welfare sessions. This combination led to fruitful discussions, new collaborations as well as in the formation of a working group that will review and compile evidence-based husbandry guidelines for the local authorities. The success of this meeting emphasizes in general that smaller local conferences provide an excellent platform to establish local networks, to build up and share local infrastructures as well as to provide knowledge and help to peer researchers.
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| 6. |
- Griffiths, Gareth Wyn, et al.
(author)
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Fish from Head to Tail : The 9th European Zebrafish Meeting in Oslo
- 2016
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In: Zebrafish. - : Mary Ann Liebert Inc. - 1545-8547 .- 1557-8542. ; 13:2, s. 132-137
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Journal article (peer-reviewed)abstract
- The 9th European Zebrafish Meeting took place recently in Oslo (June 28-July 2, 2015). A total of 650 participants came to hear the latest research news focused on the zebrafish, Danio rerio, and to its distant evolutionary relative medaka, Oryzias latipes. The packed program included keynote and plenary talks, short oral presentations and poster sessions, workshops, and strategic discussions. The meeting was a great success and revealed dramatically how important the zebrafish in particular has become as a model system for topics, such as developmental biology, functional genomics, biomedicine, toxicology, and drug development. A new emphasis was given to its potential as a model for aquaculture, a topic of great economic interest to the host country Norway and for the future global food supply in general. Zebrafish husbandry as well as its use in teaching were also covered in separate workshops. As has become a tradition in these meetings, there was a well-attended Wellcome Trust Sanger Institute and ZFIN workshop focused on Zebrafish Genome Resources on the first day. The full EZM 2015 program with abstracts can be read and downloaded from the EZM 2015 Web site zebrafish2015.org.
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| 7. |
- Kozłowski, Tomasz M, et al.
(author)
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Osmotic Concentration of Zebrafish (Danio rerio) Body Fluids is Lower in Larvae than in Adults
- 2018
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In: Zebrafish. - : Mary Ann Liebert Inc. - 1545-8547 .- 1557-8542. ; 15:1, s. 9-14
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Journal article (peer-reviewed)abstract
- We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced the best results. Immersed lenses performed better and the fixed eyes had a more natural shape. Our findings indicate that zebrafish body fluids have lower osmolality in larvae than in adults. This is probably due to an unfavorable body surface-to-volume ratio and incompletely developed regulatory mechanisms. Body fluid osmolality deviating from the adult value has to be taken into account in optical and histological work.
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| 8. |
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| 9. |
- Lendahl, M. A., et al.
(author)
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The Nordic Countries Meeting on the Zebrafish as a Model for Development and Disease 2012
- 2013
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In: Zebrafish. - : Mary Ann Liebert Inc. - 1545-8547 .- 1557-8542. ; 10:1, s. 124-125
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Journal article (other academic/artistic)abstract
- The first Nordic Countries Meeting on the Zebrafish as a Model for Development and Disease took place at Karolinska Institutet in Stockholm, November 21–23, 2012. The meeting gathered 130 scientists, students, and company representatives from Iceland, Finland, Norway, Denmark, and Sweden, as well as invited guests and keynote speakers from England, Scotland, Germany, Poland, The Netherlands, Singapore, Japan, and the United States. Presentations covered a wide range of topics, including developmental biology, genetics, evolutionary biology, toxicology, behavioral studies, and disease mechanisms. The need for formal guidance and training in zebrafish housing, husbandry, and health monitoring was recognized, and the meeting expressed its support for the joint working group of the FELASA/COST action BM0804 EuFishBioMed. The decision was made to turn the Nordic meeting into an annual event and create a Nordic network of zebrafish researchers.
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| 10. |
- Sundin, Josefin, 1981-, et al.
(author)
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On the Observation of Wild Zebrafish (Danio rerio) in India
- 2019
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In: Zebrafish. - : Mary Ann Liebert Inc. - 1545-8547 .- 1557-8542. ; 16:6, s. 546-553
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Journal article (peer-reviewed)abstract
- Zebrafish is one of the world's most widely used laboratory species, and it is utilized to answer important research questions in disparate fields such as biomedicine, genetics, developmental biology, pharmacology, toxicology, physiology, and evolution. Despite their popularity, very little is known about the biology of zebrafish in their natural habitat. This may, in part, be due to the difficulties associated with undertaking field trips to the remote areas of northern India, Nepal, and Bangladesh, which is the natural distribution range of zebrafish. Here, we present a field report describing a recent trip where we, together with local collaborators, visited several rivers in West Bengal, India, to observe wild zebrafish and their habitat. We present an overview of our observations on the biology of wild zebrafish, and the great variability of the different environments where they were found. We also include data collected on water chemistry parameters at 12 zebrafish sites, and weight data and photos of fish from these sites. We present extensive underwater videos of wild zebrafish and photographs of the sites, including video footage of courtship behavior. We show that the breeding period of wild zebrafish can be extended from the previous record of April-August to April-October. In addition, we provide practical advice for future zebrafish expeditions to this rural and inaccessible area. The goals of this article are to shed some light on the ecology of wild zebrafish, and to facilitate scientists in their future research trips. We hope that by observing zebrafish in the wild, we can increase our understanding of the natural ecology of this important model organism.
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| 11. |
- Wright, Dominic, et al.
(author)
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Social organization, grouping, and domestication in fish
- 2006
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In: Zebrafish. - : Mary Ann Liebert Inc. - 1545-8547 .- 1557-8542. ; 3:2, s. 141-155
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Research review (peer-reviewed)abstract
- The zebrafish (Danio rerio) is now established as one of the pre-eminent model vertebrate study animals in biology, providing an excellent opportunity to integrate the fields of genetics and behavioral ecology. Considerable attention has been paid to the social organization of animals and the study of zebrafish in this context allows behavioral geneticists to gain an insight into this fundamentally important field. This paper reviews the literature on the social organization of fish, with special reference to the zebrafish. The mechanisms and functions of social behavior in fish and the current understanding of the behavioral genetics of these are discussed. Finally, the impact of domestication on the expression of wild-type behavior patterns in laboratory fish is considered. © Mary Ann Liebert, Inc.
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