1. |
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2. |
- Beal, Jacob, et al.
(author)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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3. |
- Klionsky, Daniel J., et al.
(author)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
-
In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
-
Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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4. |
- Kristanl, Matej, et al.
(author)
-
The Seventh Visual Object Tracking VOT2019 Challenge Results
- 2019
-
In: 2019 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW). - : IEEE COMPUTER SOC. - 9781728150239 ; , s. 2206-2241
-
Conference paper (peer-reviewed)abstract
- The Visual Object Tracking challenge VOT2019 is the seventh annual tracker benchmarking activity organized by the VOT initiative. Results of 81 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis as well as the standard VOT methodology for long-term tracking analysis. The VOT2019 challenge was composed of five challenges focusing on different tracking domains: (i) VOT-ST2019 challenge focused on short-term tracking in RGB, (ii) VOT-RT2019 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2019 focused on long-term tracking namely coping with target disappearance and reappearance. Two new challenges have been introduced: (iv) VOT-RGBT2019 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2019 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2019, VOT-RT2019 and VOT-LT2019 datasets were refreshed while new datasets were introduced for VOT-RGBT2019 and VOT-RGBD2019. The VOT toolkit has been updated to support both standard short-term, long-term tracking and tracking with multi-channel imagery. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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5. |
- Kristan, Matej, et al.
(author)
-
The first visual object tracking segmentation VOTS2023 challenge results
- 2023
-
In: 2023 IEEE/CVF International conference on computer vision workshops (ICCVW). - : Institute of Electrical and Electronics Engineers Inc.. - 9798350307443 - 9798350307450 ; , s. 1788-1810
-
Conference paper (peer-reviewed)abstract
- The Visual Object Tracking Segmentation VOTS2023 challenge is the eleventh annual tracker benchmarking activity of the VOT initiative. This challenge is the first to merge short-term and long-term as well as single-target and multiple-target tracking with segmentation masks as the only target location specification. A new dataset was created; the ground truth has been withheld to prevent overfitting. New performance measures and evaluation protocols have been created along with a new toolkit and an evaluation server. Results of the presented 47 trackers indicate that modern tracking frameworks are well-suited to deal with convergence of short-term and long-term tracking and that multiple and single target tracking can be considered a single problem. A leaderboard, with participating trackers details, the source code, the datasets, and the evaluation kit are publicly available at the challenge website1
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6. |
- Kristan, Matej, et al.
(author)
-
The Visual Object Tracking VOT2015 challenge results
- 2015
-
In: Proceedings 2015 IEEE International Conference on Computer Vision Workshops ICCVW 2015. - : IEEE. - 9780769557205 ; , s. 564-586
-
Conference paper (peer-reviewed)abstract
- The Visual Object Tracking challenge 2015, VOT2015, aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 62 trackers are presented. The number of tested trackers makes VOT 2015 the largest benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the appendix. Features of the VOT2015 challenge that go beyond its VOT2014 predecessor are: (i) a new VOT2015 dataset twice as large as in VOT2014 with full annotation of targets by rotated bounding boxes and per-frame attribute, (ii) extensions of the VOT2014 evaluation methodology by introduction of a new performance measure. The dataset, the evaluation kit as well as the results are publicly available at the challenge website(1).
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7. |
- Lu, Yingying, et al.
(author)
-
Telomere dysfunction promotes small vessel vasculitis via the LL37-NETs-dependent mechanism
- 2020
-
In: Annals of Translational Medicine. - : AME PUBL CO. - 2305-5839 .- 2305-5847. ; 8:6
-
Journal article (peer-reviewed)abstract
- Background: Small vessel vasculitis (SVV) is a group of systemic autoimmune diseases that are mediated by neutrophil extracellular traps (NETs) in response to cathelicidin LL37, an aging molecular marker, which could be induced by telomere dysfunction. Therefore, in this study, we evaluated the hypothesis that telomere dysfunction in neutrophils may promote SVV via an LL37-NETs-dependent mechanism. Methods: We contrasted the release of neutrophil NETs from mice with telomere dysfunction, mice with DNA damage and wide-type mice. Neutrophil telomere length, the expression of LL37, and the formation of NETs were measured in SVV patients and healthy controls (HCs). The co-expression of gamma H2AX, LL37, and NETs were detected in SVV patients to evaluate the association of the immune aging of neutrophils and pro-inflammatory conditions. LL37 inhibitor was used to verify its key role in NETs release in SVV patients and DNA damage mice. Results: We found that NETs were over-induced by telomere dysfunction and DNA damage in mice, which may be associated with a marked increase in LL37. For patients with SVV, telomeres in neutrophils were significantly shortened, which was also associated with higher levels of LL37 and NETs. Inhibition of LL37 reduced the NETs released from neutrophils. Conclusions: Taken together, the results of these studies suggest that dysfunction of telomeres may promote SVV through the mechanism of LL37-dependent NETs. Thus, targeting the LL37-NETs may be a novel therapy for SVV.
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8. |
- Wang, Fang, et al.
(author)
-
Emerging contaminants: A One Health perspective
- 2024
-
In: Innovation. - 2666-6758. ; 5
-
Research review (peer-reviewed)abstract
- Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary health. Despite global efforts to mitigate legacy pollutants, the continuous introduction of new substances remains a major threat to both people and the planet. In response, global initiatives are focusing on risk assessment and regulation of emerging contaminants, as demonstrated by the ongoing efforts to establish the UN's Intergovernmental Science-Policy Panel on Chemicals, Waste, and Pollution Prevention. This review identifies the sources and impacts of emerging contaminants on planetary health, emphasizing the importance of adopting a One Health approach. Strategies for monitoring and addressing these pollutants are discussed, underscoring the need for robust and socially equitable environmental policies at both regional and international levels. Urgent actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.
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9. |
- Zhu, Zhenyu, et al.
(author)
-
Coupling life prediction of bending very high cycle fatigue of completion strings made of different materials using deep wise separable convolution
- 2024
-
In: Fatigue & Fracture of Engineering Materials & Structures. - : WILEY. - 8756-758X .- 1460-2695.
-
Journal article (peer-reviewed)abstract
- This article predicts bending very high cycle fatigue (VHCF) life of three typical nickel-based alloys SM2550, BG2532, and G3 used for completion strings. Fatigue tests were conducted on the three alloys using an ultrasonic fatigue system at a frequency of 20 kHz. The results showed that the fatigue strength ranges of the three alloys were markedly different, reflecting their different sensitivities to fatigue loading. Scanning electron microscope observations revealed numerous fatigue crack origins with internal decohesion in the fatigue source region. To achieve unified prediction of the fatigue life for the three alloys, a prediction model based on deep learning was built with inputs including fatigue initiation quantity, cleavage facet size, and other fatigue fracture characteristics. It was found that single source feature was insufficient to obtain satisfactory prediction accuracy for all alloys, while multifeature coupling integration could significantly improve the prediction precision, enabling reliable prediction of alloy fatigue life. This study provides new insights into bending VHCF life prediction. This article predicts bending VHCF life for three completion strings. Bending VHCF life model utilizing deep wise separable convolution was established. Deep learning can effectively integrate with bending VHCF analyses.
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10. |
- Archambault, Emile, et al.
(author)
-
Routing and spectrum assignment in elastic filterless optical networks
- 2016
-
In: IEEE/ACM Transactions on Networking. - : IEEE. - 1063-6692 .- 1558-2566. ; 24:6, s. 3578-3592
-
Journal article (peer-reviewed)abstract
- Elastic optical networking is considered a promising candidate to improve the spectral efficiency of optical networks. One of the most important planning challenges of elastic optical networks is the NP-hard routing and spectrum assignment (RSA) problem. In this paper, we investigate offline RSA in elastic filterless optical networks, which use a passive broadcast-and-select architecture to offer network agility. Here elastic optical network is referred to as the optical network that can adapt the channel bandwidth, data rate, and transmission format for each traffic demand in order to offer maximum throughput. In elastic filterless networks, the presence of unfiltered signals resulting from the drop-and-continue node architecture must be considered as an additional constraint in the RSA problem. In this paper, first the RSA problem in elastic filterless networks is formulated by using integer linear program (ILP) to obtain optimal solutions for small networks. Due to the problem complexity, two efficient RSA heuristics are also proposed to achieve suboptimal solutions for larger networks in reasonable time. Simulation results show that significant bandwidth savings in elastic filterless networks can be achieved compared to the fixed-grid filterless solutions. The proposed approach is further tested in multi-period traffic scenarios and combined with periodical spectrum defragmentation, leading to additional improvement in spectrum utilization of elastic filterless optical networks.
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11. |
- Chen, Jiajia, et al.
(author)
-
Passive wide area network solutions : Filterless and semi-filterless optical networks
- 2011
-
In: 13th International Conference on Transparent Optical Networks (ICTON). - 9781457708800
-
Conference paper (peer-reviewed)abstract
- Advances in optical coherent transmission and electrical compensation technologies (such as coherent receiver and forward error correction FEC) have stimulated ideas for novel optical network architectures. Recently proposed passive wide area network solution, referred to as filterless optical network [1 #x2013;2] eliminates or minimizes the usage of active photonic reconfigurable network elements. In this approach, only the passive splitters and combiners for interconnecting the fiber links are utilized, which makes this network architecture more cost- and energy-effective as well as more reliable compared with networks based on active optical switching. However, the filterless optical network architecture implies some constraints on fiber interconnection design, maximum fiber-tree length and wavelength reuse due to its broadcast nature. Consequently, filterless solution always requires more resources (i.e. number of wavelengths) compared with the active switched optical networks which are allowed to utilize reconfigurable and coloured components. In order to improve the wavelength utilization while maintaining flexibility of resource allocation, this work extends the idea of filterless optical network by introducing some passive coloured components (e.g., fiber Bragg grating FBG, red/blue filters, etc) to drop local signals at some determined nodes. This approach is referred to as semi-filterless optical network. Furthermore, the semi-filterless solution maintains the passive feature, enabling high reliability and efficiency of cost and energy. Meanwhile, its non-broadcast property at some determined nodes has potential to decrease the transmission impairments and hence relax the constraints on fiber interconnection design and the maximal transparent length, which are strict in the filterless optical network. Our preliminary results confirm the advantages of semi-filterless solution.
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12. |
- Chen, Leilei, et al.
(author)
-
High-temperature wear mechanisms of TiNbWN films : Role of nanocrystalline oxides formation
- 2023
-
In: Friction. - : Springer. - 2223-7690 .- 2223-7704. ; 10
-
Journal article (peer-reviewed)abstract
- Refractory high/medium entropy nitrides (HENs/MENs) exhibit comprehensive application prospects as protective films on mechanical parts, particularly those subjected to sliding contacts at elevated temperatures. In this study, a new MEN system TiNbWN, forming a single fcc solution, is designed and its wear performance at temperatures ranging from 25 to 750 °C is explored. The wear mechanisms can be rationalized by examining the subsurface microstructural evolutions using the transmission electron microscopy as well as calculating the phase diagrams and interfacial adhesion behavior employing calculation of phase diagram (CALPHAD) and density functional theory (DFT). To be specific, increased wear losses occur in a temperature range of 25–600 °C, being predominantly caused by the thermally-induced hardness degradation; whereas at the ultimate temperature (750 °C), the wear loss is refrained due to the formation of nanocrystalline oxides (WnO3n−2, TiO2, and γTiOx), as synergistically revealed by microscopy and CALPHAD, which not only enhance the mechanical properties of the pristine nitride film, but also act as solid lubricants, reducing the interfacial adhesion. Thus, our work delineates the role of the in situ formed nanocrystalline oxides in the wear mechanism transition of TiNbWN thin films, which could shed light on the high-temperature wear behavior of refractory HEN/MEN films.
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13. |
- Ji, Zhenyu, et al.
(author)
-
MITF Modulates Therapeutic Resistance through EGFR Signaling.
- 2015
-
In: Journal of Investigative Dermatology. - : Elsevier BV. - 1523-1747 .- 0022-202X. ; 135:7, s. 1863-1872
-
Journal article (peer-reviewed)abstract
- Response to targeted therapies varies significantly despite shared oncogenic mutations. Nowhere is this more apparent than in BRAF(V600E)-mutated melanomas where initial drug response can be striking and yet relapse is commonplace. Resistance to BRAF inhibitors have been attributed to the activation of various receptor tyrosine kinases (RTKs) though the underlying mechanisms have been largely uncharacterized. Here, we found that EGFR induced vemurafenib resistance is ligand dependent. We then employed whole-genome expression analysis and discovererd that vemurafenib resistance correlated with the loss of MITF, along with its melanocyte lineage program, and with the activation of EGFR signaling. An inverse relationship between MITF, vemurafenib resistance and EGFR was then observed in patient samples of recurrent melanoma and was conserved across melanoma cell lines and patients' tumor specimens. Functional studies revealed that MITF depletion activated EGFR signaling and consequently recapitulated the resistance phenotype. In contrast, forced expression of MITF in melanoma and colon cancer cells inhibited EGFR and conferred sensitivity to BRAF/MEK inhibitors. These findings indicate that an "autocrine drug resistance loop" is suppressed by melanocyte lineage signal(s), such as MITF. This resistance loop modulates drug response and could explain the unique sensitivity of melanomas to BRAF inhibition.Journal of Investigative Dermatology accepted article preview online, 19 March 2015. doi:10.1038/jid.2015.105.
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14. |
- Ji, Zhenyu, et al.
(author)
-
Vemurafenib Synergizes with Nutlin-3 to Deplete Survivin and Suppresses Melanoma Viability and Tumor Growth
- 2013
-
In: Clinical Cancer Research. - 1078-0432. ; 19:16, s. 4383-4391
-
Journal article (peer-reviewed)abstract
- Purpose: For patients with advanced melanoma, primary and secondary resistance to selective BRAF inhibition remains one of the most critically compelling challenges. One rationale argues that novel biologically informed strategies are needed to maximally cripple melanoma cells up front before compensatory mechanisms emerge. As p53 is uncommonly mutated in melanoma, restoration of its function represents an attractive adjunct to selective BRAF inhibition. Experimental Design: Thirty-seven BRAF(V600E)-mutated melanoma lines were subjected to synergy studies in vitro using a combination of vemurafenib and nutlin-3 (Nt-3). In addition, cellular responses and in vivo efficacy were also determined. We also analyzed changes in the levels of canonical apoptotic/survival factors in response to vemurafenib. Results: Dual targeting of BRAF(V600E) and Hdm2 with vemurafenib and Nt-3, respectively, synergistically induced apoptosis and suppressed melanoma viability in vitro and tumor growth in vivo. Suppression of p53 in melanoma cells abrogated Nt-3's effects fully and vemurafenib's effects partially. A survey of canonical survival factors revealed that both vemurafenib and Nt-3 independently attenuated levels of the antiapoptotic protein, survivin. Genetic depletion of survivin reproduces the cytotoxic effects of the combination strategy. Conclusion: These results show preclinical feasibility for overcoming primary vemurafenib resistance by restoring p53 function. Moreover, it identifies survivin as one downstream mediator of the observed synergism and a potential secondary target. (C)2013 AACR.
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15. |
- Klionsky, Daniel J., et al.
(author)
-
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
- 2008
-
In: Autophagy. - : Landes Bioscience. - 1554-8627 .- 1554-8635. ; 4:2, s. 151-175
-
Research review (peer-reviewed)abstract
- Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
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16. |
- Kristan, Matej, et al.
(author)
-
The Visual Object Tracking VOT2016 Challenge Results
- 2016
-
In: COMPUTER VISION - ECCV 2016 WORKSHOPS, PT II. - Cham : SPRINGER INT PUBLISHING AG. - 9783319488813 - 9783319488806 ; , s. 777-823
-
Conference paper (peer-reviewed)abstract
- The Visual Object Tracking challenge VOT2016 aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 70 trackers are presented, with a large number of trackers being published at major computer vision conferences and journals in the recent years. The number of tested state-of-the-art trackers makes the VOT 2016 the largest and most challenging benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the Appendix. The VOT2016 goes beyond its predecessors by (i) introducing a new semi-automatic ground truth bounding box annotation methodology and (ii) extending the evaluation system with the no-reset experiment.
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17. |
- Liao, Yaoming, et al.
(author)
-
Downscaling of future precipitation in china’s beijing-tianjin-hebei region using a weather generator
- 2022
-
In: Atmosphere. - : MDPI AG. - 2073-4433. ; 13:1
-
Journal article (peer-reviewed)abstract
- To project local precipitation at the existing meteorological stations in China’s Beijing-Tianjin-Hebei region in the future, local daily precipitation was simulated for three periods (2006–2030, 2031–2050, and 2051–2070) under RCP 4.5 and RCP 8.5 emission scenarios. These projections were statistically downscaled using a weather generator (BCC/RCG-WG) and the output of five global climate models. Based on the downscaled daily precipitation at 174 stations, eight indices describing mean and extreme precipitation climates were calculated. Overall increasing trends in the frequency and intensity of the mean and extreme precipitation were identified for the majority of the stations studied, which is in line with the GCMs’ output. However, the downscaling approach enables more local features to be reflected, adding value to applications at the local scale. Compared with the baseline during 1961–2005, the regional average annual precipitation and its intensity are projected to increase in all three future periods under both RCP 4.5 and RCP 8.5. The projected changes in the number of days with precipitation are relatively small across the Beijing-Tianjin-Hebei region. The regional average annual number of days with precipitation would increase by 0.2~1.0% under both RCP 4.5 and RCP 8.5, except during 2031–2050 under RCP 8.5 when it would decrease by 0.7%. The regional averages of annual days with precipitation ≥25 mm and ≥40 mm, the greatest one-day and five-day precipitation in the Beijing-Tianjin-Hebei region, are projected to increase by 8~30% during all the three periods. The number of days with daily precipitation ≥40 mm was projected to increase most significantly out of the eight indices, indicating the need to consider increased flooding risk in the future. The average annual maximum number of consecutive days without precipitation in the Beijing-Tianjin-Hebei region is projected to decrease, and the drought risk in this area is expected to decrease.
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18. |
- Marinins, Aleksandrs, et al.
(author)
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Photostable Polymer/Si Nanocrystal Bulk Hybrids with Tunable Photoluminescence
- 2016
-
In: ACS Photonics. - : American Chemical Society (ACS). - 2330-4022. ; 3:9, s. 1575-1580
-
Journal article (peer-reviewed)abstract
- Solid polymer/Si nanocrystal bulk nanocomposites were fabricated from solutions of alkene- and hydride-terminated silicon nanocrystals (NCs) in toluene. The photoluminescence peak position of hydride-terminated SiNCs before polymerization was tuned by photoassisted hydrofluoric acid etching. Optical properties of obtained PMMA/NC hybrids, such as quantum yield, luminescence lifetime, and dispersion factor, were evaluated over time. Photostability of these transparent bulk polymer/SiNC hybrids over months was confirmed. The emission covers the visible to near-infrared range with a quantum yield of similar to 30-40% for yellow-red nanocomposites.
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19. |
- Miao, Yuchen, et al.
(author)
-
TouchKey : Touch to Generate Symmetric Keys by Skin Electric Potentials Induced by Powerline Radiation
- 2023
-
In: Proceedings of the ACM on Interactive Mobile Wearable and Ubiquitous Technologies-Imwut. - : Association for Computing Machinery (ACM). - 2474-9567. ; 7:2
-
Journal article (peer-reviewed)abstract
- Secure device pairing is important to wearables. Existing solutions either degrade usability due to the need of specific actions like shaking, or they lack universality due to the need of dedicated hardware like electrocardiogram sensors. This paper proposes TOUCHKEY, a symmetric key generation scheme that exploits the skin electric potential (SEP) induced by powerline electromagnetic radiation. The SEP is ubiquitously accessible indoors with analog-to-digital converters widely available on Internet of Things devices. Our measurements show that the SEP has high randomness and the SEPs measured at two close locations on the same human body are similar. Extensive experiments show that TOUCHKEY achieves a high key generation rate of 345 bit/s and an average success rate of 99.29%. Under a range of adversary models including active and passive attacks, TOUCHKEY shows a low false acceptance rate of 0.86%, which outperforms existing solutions. Besides, the overall execution time and energy usage are 0.44 s and 2.716 mJ, which make it suitable for resource-constrained devices.
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20. |
- Ning, Rui, et al.
(author)
-
Assessing progression limits in different grades of keratoconus from a novel perspective : precision of measurements of the corneal epithelium
- 2024
-
In: Eye and Vision. - 2326-0254. ; 11:1
-
Journal article (peer-reviewed)abstract
- Background: To assess repeatability and reproducibility of corneal epithelium thickness (ET) measured by a spectral-domain optical coherence tomographer (SD-OCT)/Placido topographer (MS-39, CSO, Florence, Italy) in keratoconus (KC) population at different stages, as well as to determine the progression limits for evaluating KC progression. Methods: A total of 149 eyes were enrolled in this study, with 29 eyes in the forme fruste keratoconus (FFKC) group, 34 eyes in the mild KC group, 40 eyes in the moderate KC group, and 46 eyes in the severe KC group. Employing the within-subject standard deviation (Sw), test-retest variability (TRT), coefficient of variation (CoV), and intraclass correlation coefficient (ICC) to evaluate intraoperator repeatability and interoperator reproducibility. Results: The repeatability and reproducibility of MS-39 in patients with KC were acceptable, according to ICC values ranging from 0.732 to 0.954. However, patients with more severe KC and progressive peripheralization of the measurement points had higher TRTs but a thinning trend. The current study tended to set the cut-off values of mild KC, moderate KC, and severe KC to 4.9 µm, 5.2 µm, and 7.4 µm for thinnest epithelium thickness (TET). When differences between follow-ups are higher than those values, progression of the disease is possible. As for center epithelium thickness (CET), cut-off values for mild KC, moderate KC, and severe KC should be 2.8 µm, 4.4 µm, and 5.3 µm. This might be useful in the follow-up and diagnosis of keratoconus. Conclusions: This study demonstrated that the precision of MS-39 was reduced in measuring more severe KC patients and more peripheral corneal points. In determining disease progression, values should be differentiated between disease-related real changes and measurement inaccuracies. Due to the large difference in ET measured by MS-39 between various stages of disease progression, it is necessary to accurately grade KC patients to avoid errors in KC clinical decision-making.
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21. |
- Nooruzzaman, Md., et al.
(author)
-
Filterless Architecture for Coherent Undersea Networks
- 2015
-
In: 2015 INTERNATIONAL CONFERENCE ON OPTICAL NETWORK DESIGN AND MODELING (ONDM). - : IEEE. - 9783901882722 ; , s. 68-73
-
Conference paper (peer-reviewed)abstract
- Active photonic switching components, such as reconfigurable optical add/drop multiplexers (ROADMs), cannot be deployed at the branching units (BUs) in submarine networks, which limits the flexibility of the undersea networks. In this regard, the filterless optical network architecture based on passive broadcast-and-select nodes and coherent transceivers at the edge terminals can be considered as a promising solution. In this paper we propose and evaluate a filterless network architecture for a trunk and branch undersea network topology. The performance is compared with a conventional solution in terms of cost and wavelength consumption. And it is shown that the filterless architecture can bring significant cost savings for both the terminals and line equipment while offering the same agility as the conventional network architecture.
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22. |
- Qi, Zhenyu, et al.
(author)
-
Blueshifting the Absorption of a Small -Molecule Donor and Using it as the Third Component to Achieve High-Efficiency Ternary Organic Solar Cells
- 2022
-
In: Solar RRL. - : WILEY-V C H VERLAG GMBH. - 2367-198X. ; 6:9
-
Journal article (peer-reviewed)abstract
- Adding a small-molecule donor (SMD) to state-of-the-art nonfullerene organic solar cells (OSCs) is demonstrated as a useful strategy to construct ternary organic solar cells, as SMDs typically have high crystallinity and can tune charge transport properties of OSCs. However, the absorption of most SMDs overlaps with typical donor polymers (e.g., PM6), which is against the general guidelines of adopting materials with complementary absorption in ternary OSCs. Herein, the absorption of state-of-art SMDs (BTR-CI) by linking the beta position of the outer thiophene to the alpha position of the inner thiophene unit is intentionally blueshifted. The resulting molecule beta-S1 shows a maximum absorption peak at 505 nm in the film state, which exhibits wider bandgap and shows complementary absorption with the host system (PM6:Y6). The corresponding ternary OSCs with 20%wt beta-S1 show significantly enhanced efficiency from 16.2% to 17.1% due to the increased short-circuit current (J(sc)) and improved fill factor (FF). Herein, an effective strategy to design SMDs with both wider bandgaps and higher crystallinity for high-performance ternary OSCs is presented.
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23. |
- Tremblay, Christine, et al.
(author)
-
Proposed filterless architecture and control plane for emerging flexible coherent networks
- 2013
-
In: ICTON 2013. - 9781479906833 ; , s. 1-1
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Conference paper (peer-reviewed)abstract
- Recent developments in coherent modem performance and digital signal processing (DSP) technologies have opened the opportunity for new agile network architectures [1, 2]. The filterless network concept has been proposed in [3] and it is shown that they are more cost-effective and reliable compared to active optical switching networks. Filterless optical networks use broadcast-and-select techniques in which passive non-filtered optical splitters and combiners are used for channel add-drop and fiber link interconnection. Furthermore, DSP-based coherent modems are complementary foundational technologies for flexible optical networking owing to their properties of dynamic impairment compensation, performance monitoring and tuneability. The resulting network architecture makes an attractive candidate solution for flexible optical networking [4]. In this talk, we review the recent progress in filterless optical network design and control [5]ᅵ[7]. In the first part, filterless architectural solutions are proposed for different network topologies and compared to active photonic switching solutions. A cost and performance analysis of filterless network solutions with 1 + 1 optical layer protection is also presented. In the second part, we present a control plane for filterless optical networks and describe its main characteristics through a performance study. Our results show that passive filterless networks can be considered as a cost effective and simpler alternative to active optical switching networks whenever traffic loading is not approaching full network capacity.
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24. |
- Xu, Zhe, et al.
(author)
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Absorption and transport of a Mytilus edulis-derived peptide with the function of preventing osteoporosis
- 2021
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In: Food & Function. - : Royal Society of Chemistry. - 2042-6496 .- 2042-650X. ; 12:5, s. 2102-2111
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Journal article (peer-reviewed)abstract
- The YPRKDETGAERT peptide (PME-1) identified from the Mytilus edulis proteins has been shown to promote the proliferation and differentiation of osteoblasts and it has good bone-forming activity in vitro. Further, PME-1 has been shown to prevent osteoporosis in vivo. PME-1 can be absorbed through the gastrointestinal tract, and the passing rate in monolayer Caco-2 cells was 6.57%. PME-1 can also enter the blood circulation and the concentration of PME-1 in serum reached the maximum, 61.06 +/- 26.32 ng mL(-1), 20 min after feeding. The multifunctional in vivo imager was used to further determine the distribution of the 5-FITC-(Acp)-YPRKDETGAERT peptide (PME-1-FITC) 2 h after feeding the peptide, and the result confirmed the above results and showed that a part of PME-1-FITC can affect bone in vivo. Therefore, PME-1 not only was easily absorbed in the gastrointestinal tract, but also has the potential beneficial effect on preventing osteoporosis.
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25. |
- Xu, Zhenyu, et al.
(author)
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Flexible Bandwidth Allocation in Filterless Optical Networks
- 2015
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In: IEEE Communications Letters. - 1089-7798 .- 1558-2558. ; 19:4, s. 565-568
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Journal article (peer-reviewed)abstract
- We introduce the new concept of an elastic filterless optical network and propose an efficient heuristic algorithm for solving the static routing and spectrum assignment problem. Our simulation results obtained for different network topologies under multi-period traffic show increasing bandwidth savings with the growth of traffic load compared to a fixed-grid scenario. We also show the benefits of periodical spectrum defragmentation.
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