| 1. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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- 2019
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Journal article (peer-reviewed)
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| 3. |
- Abbafati, Cristiana, et al.
(author)
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- 2020
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Journal article (peer-reviewed)
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| 4. |
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| 5. |
- Bao, Haiyan, et al.
(author)
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Condition of setting surge tanks in hydropower plants - A review
- 2018
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In: Renewable & sustainable energy reviews. - : Elsevier BV. - 1364-0321 .- 1879-0690. ; 81, s. 2059-2070
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Research review (peer-reviewed)abstract
- Hydropower plays an important role in the safe, stable and efficient operation of power systems, especially with current trends toward renewable energy systems. The total global potential of gross, technical, economic, and exploitable hydropower are still enormous in the future, and the developments of new hydropower stations (HPSs) are of great importance. For constructions of new HPSs, the condition of setting surge tanks (CSST) is crucial for various perspectives, e.g. safety, stability and economy of HPSs. In this review, the CSST are summarized and analyzed from the three aspects: regulation assurance, operation stability, and the regulation quality, with an aim of providing a reference and guidance for research and engineering applications regarding surge tanks. Upstream and downstream surge tanks in conventional HPSs and pumped storage power stations are all included. Moreover, a comprehensive comparison of CSST under different conditions is conducted. One of the main focuses of this review is on Chinese studies, for introducing many meaningful results written in Chinese to more readers all over the world.
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| 6. |
- Cui, Liang, et al.
(author)
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Four additional natural 7-deazaguanine derivatives in phages and how to make them
- 2023
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In: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 51:17, s. 9214-9226
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Journal article (peer-reviewed)abstract
- Bacteriophages and bacteria are engaged in a constant arms race, continually evolving new molecular tools to survive one another. To protect their genomic DNA from restriction enzymes, the most common bacterial defence systems, double-stranded DNA phages have evolved complex modifications that affect all four bases. This study focuses on modifications at position 7 of guanines. Eight derivatives of 7-deazaguanines were identified, including four previously unknown ones: 2 & PRIME;-deoxy-7-(methylamino)methyl-7-deazaguanine (mdPreQ(1)), 2 & PRIME;-deoxy-7-(formylamino)methyl-7-deazaguanine (fdPreQ(1)), 2 & PRIME;-deoxy-7-deazaguanine (dDG) and 2 & PRIME;-deoxy-7-carboxy-7-deazaguanine (dCDG). These modifications are inserted in DNA by a guanine transglycosylase named DpdA. Three subfamilies of DpdA had been previously characterized: bDpdA, DpdA1, and DpdA2. Two additional subfamilies were identified in this work: DpdA3, which allows for complete replacement of the guanines, and DpdA4, which is specific to archaeal viruses. Transglycosylases have now been identified in all phages and viruses carrying 7-deazaguanine modifications, indicating that the insertion of these modifications is a post-replication event. Three enzymes were predicted to be involved in the biosynthesis of these newly identified DNA modifications: 7-carboxy-7-deazaguanine decarboxylase (DpdL), dPreQ(1) formyltransferase (DpdN) and dPreQ(1) methyltransferase (DpdM), which was experimentally validated and harbors a unique fold not previously observed for nucleic acid methylases.
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| 7. |
- Prokopenko, Inga, et al.
(author)
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A Central Role for GRB10 in Regulation of Islet Function in Man.
- 2014
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In: PLoS Genetics. - : Public Library of Science (PLoS). - 1553-7404 .- 1553-7390. ; 10:4
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Journal article (peer-reviewed)abstract
- Variants in the growth factor receptor-bound protein 10 (GRB10) gene were in a GWAS meta-analysis associated with reduced glucose-stimulated insulin secretion and increased risk of type 2 diabetes (T2D) if inherited from the father, but inexplicably reduced fasting glucose when inherited from the mother. GRB10 is a negative regulator of insulin signaling and imprinted in a parent-of-origin fashion in different tissues. GRB10 knock-down in human pancreatic islets showed reduced insulin and glucagon secretion, which together with changes in insulin sensitivity may explain the paradoxical reduction of glucose despite a decrease in insulin secretion. Together, these findings suggest that tissue-specific methylation and possibly imprinting of GRB10 can influence glucose metabolism and contribute to T2D pathogenesis. The data also emphasize the need in genetic studies to consider whether risk alleles are inherited from the mother or the father.
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| 8. |
- Wu, Xiujuan, et al.
(author)
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Exploration of electrocatalytic water oxidation properties of NiFe catalysts doped with nonmetallic elements (P, S, Se)
- 2021
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In: International journal of hydrogen energy. - : Elsevier BV. - 0360-3199 .- 1879-3487. ; 46:79, s. 38992-39002
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Journal article (peer-reviewed)abstract
- The introduction of non-metallic atoms (P, S, Se) has emerged as an effective way to improve the catalytic activity of transition metal based layered double hydroxides (LDHs) for oxygen evolution reaction. However, objective comparisons of the performance of heteroelement-doped catalysts are complicated by the lack of standardization both in the electrochemical tests and physicochemical analysis. Herein, we use a unified protocol for evaluating the catalytic activities of heteroelement-doped NiFe-LDHs and explore the reasons for the differences in their catalytic performance. Some regular results are found from comparing the properties of the heteroelement-doped catalysts: (1) the introduction of P/S/Se can optimize the redox behaviors of Ni species, which is conducive to regulating adsorption energy of intermediates and the formation of high-valent active sites; (2) the specific area of catalysts was expanded after heteroelements doping, ensuring a more favorable structure for heterogeneous catalysis; (3) All P/S/Se-doped catalysts showed better activities when compared to the original NiFe LDHs in alkaline solutions, and the catalysts doped with S showed the best performance (Se < P < S).
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| 9. |
- Wu, Xiujuan, et al.
(author)
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Metalloid Te-Doped Fe-Based Catalysts Applied for Electrochemical Water Oxidation
- 2021
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In: CHEMISTRYSELECT. - : Wiley. - 2365-6549. ; 6:24, s. 6154-6158
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Journal article (peer-reviewed)abstract
- Metal telluride nanocatalysts have been widely used in the catalytic reactions. However, there are few examples of electrocatalytic water oxidation with metal telluride as catalysts, especially Fe-based telluride, to the best of our knowledge, has not yet been reported. In this work, the Te-doped Fe-based catalysts (FeTex) with crystalline and amorphous nanosheet structures have been successfully synthesized by chemical vapor deposition (CVD) as well as electrodeposition (ED) on the iron foam (IF), where the latter exhibited more remarkable electrocatalytic performance towards the oxygen evolution reaction (OER) in the alkaline electrolyte, requiring the overpotential (eta) of only 264.4 mV to reach a current density of 10 mA cm(-2) with a Tafel slope of 54.2 mV dec(-1). The electrochemical study confirmed that the residual Te in ED-FeTex does play a role in promoting the catalytic activity for the electrocatalytic water oxidation.
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| 10. |
- Yaghootkar, Hanieh, et al.
(author)
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Mendelian randomization studies do not support a causal role for reduced circulating adiponectin levels in insulin resistance and type 2 diabetes.
- 2013
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In: Diabetes. - : American Diabetes Association. - 1939-327X .- 0012-1797. ; 62:10, s. 3589-3598
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Journal article (peer-reviewed)abstract
- Adiponectin is strongly inversely associated with insulin resistance and type 2 diabetes, but its causal role remains controversial. We used a Mendelian randomization approach to test the hypothesis that adiponectin causally influences insulin resistance and type 2 diabetes. We used genetic variants at the ADIPOQ gene as instruments to calculate a regression slope between adiponectin levels and metabolic traits (up to 31,000 individuals) and a combination of instrumental variables and summary statistics-based genetic risk scores to test the associations with gold-standard measures of insulin sensitivity (2,969 individuals) and type 2 diabetes (15,960 case subjects and 64,731 control subjects). In conventional regression analyses, a 1-SD decrease in adiponectin levels was correlated with a 0.31-SD (95% CI 0.26-0.35) increase in fasting insulin, a 0.34-SD (0.30-0.38) decrease in insulin sensitivity, and a type 2 diabetes odds ratio (OR) of 1.75 (1.47-2.13). The instrumental variable analysis revealed no evidence of a causal association between genetically lower circulating adiponectin and higher fasting insulin (0.02 SD; 95% CI -0.07 to 0.11; N = 29,771), nominal evidence of a causal relationship with lower insulin sensitivity (-0.20 SD; 95% CI -0.38 to -0.02; N = 1,860), and no evidence of a relationship with type 2 diabetes (OR 0.94; 95% CI 0.75-1.19; N = 2,777 case subjects and 13,011 control subjects). Using the ADIPOQ summary statistics genetic risk scores, we found no evidence of an association between adiponectin-lowering alleles and insulin sensitivity (effect per weighted adiponectin-lowering allele: -0.03 SD; 95% CI -0.07 to 0.01; N = 2,969) or type 2 diabetes (OR per weighted adiponectin-lowering allele: 0.99; 95% CI 0.95-1.04; 15,960 case subjects vs. 64,731 control subjects). These results do not provide any consistent evidence that interventions aimed at increasing adiponectin levels will improve insulin sensitivity or risk of type 2 diabetes.
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