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- Liukkonen, J, et al.
(author)
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Salivary biomarkers in association with periodontal parameters and the periodontitis risk haplotype
- 2018
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In: Innate immunity. - : SAGE Publications. - 1753-4267 .- 1753-4259. ; 24:7, s. 439-447
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Journal article (peer-reviewed)abstract
- Genetic factors play a role in periodontitis. Here we examined whether the risk haplotype of MHC class III region BAT1-NFKBIL1-LTA and lymphotoxin-α polymorphisms associate with salivary biomarkers of periodontal disease. A total of 455 individuals with detailed clinical and radiographic periodontal health data were included in the study. A 610 K genotyping chip and a Sequenom platform were used in genotyping analyses. Phospholipid transfer protein activity, concentrations of lymphotoxin-α, IL-8 and myeloperoxidase, and a cumulative risk score (combining Porphyromonas gingivalis, IL-1β and matrix metalloproteinase-8) were examined in saliva samples. Elevated IL-8 and myeloperoxidase concentrations and cumulative risk scores associated with advanced tooth loss, deepened periodontal pockets and signs of periodontal inflammation. In multiple logistic regression models adjusted for periodontal parameters and risk factors, myeloperoxidase concentration (odds ratio (OR); 1.37, P = 0.007) associated with increased odds for having the risk haplotype and lymphotoxin-α concentration with its genetic variants rs2857708, rs2009658 and rs2844482. In conclusion, salivary levels of IL-8, myeloperoxidase and cumulative risk scores associate with periodontal inflammation and tissue destruction, while those of myeloperoxidase and lymphotoxin-α associate with genetic factors as well.
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| 5. |
- Liukkonen, J, et al.
(author)
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Immunological and Microbiological Profiling of Cumulative Risk Score for Periodontitis
- 2020
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In: Diagnostics (Basel, Switzerland). - : MDPI AG. - 2075-4418. ; 10:8
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Journal article (peer-reviewed)abstract
- The cumulative risk score (CRS) is a mathematical salivary diagnostic model to define an individual’s risk of having periodontitis. In order to further validate this salivary biomarker, we investigated how periodontal bacteria, lipopolysaccharide (LPS), and systemic and local host immune responses relate to CRS. Subgingival plaque, saliva, and serum samples collected from 445 individuals were used in the analyses. Plaque levels of 28 microbial species, especially those of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Tannerella forsythia, and serum and salivary levels of IgA and IgG against these five species were determined. Additionally, LPS activity was measured. High CRS associated strongly with all IgA/IgG antibody and LPS levels in saliva, whereas in serum the associations were not that obvious. In the final logistic regression model, the best predictors of high CRS were saliva IgA burden against the five species (OR 7.04, 95% CI 2.25–22.0), IgG burden (3.79, 1.78–8.08), LPS (2.19, 1.38–3.47), and the sum of 17 subgingival Gram-negative species (6.19, 2.10–18.3). CRS is strongly associated with microbial biomarker species of periodontitis and salivary humoral immune responses against them.
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- Autti, Taina, et al.
(author)
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Bone marrow transplantation in aspartylglucosaminuria : histopathological and MRI study
- 1999
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In: Neuropediatrics. - : Georg Thieme Verlag KG. - 0174-304X .- 1439-1899. ; 30:6, s. 283-8
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Journal article (peer-reviewed)abstract
- This study comprised two patients with aspartylglucosaminuria (AGU), who were followed up for 4 and 7 years. The patients underwent allogeneic bone marrow transplantation (BMT) at the ages of 2 and 2.6 years. Both patients had abnormal speech development and gross motor clumsiness. At the time of the BMT, they were mentally retarded. We report on follow-up data of these patients obtained by MRI, in addition to the histopathological, biochemical and clinical investigations. MR images of six non-transplanted patients and seven healthy children served as controls. In the non-transplanted patients, MRI revealed evident delay of myelination in contrast to the two transplanted patients showing fair or evident grey- vs. white matter differentiation on T2-weighted images. The aspartylglucosaminidase (AGA) activity in blood leukocytes reached a heterozygous level. Urinary excretion of aspartylglucosamine and glycoasparagines slowly decreased but remained about a third of the pre-BMT level 5 years after BMT. Storage lysosomes in electron microscopic investigations were not decreased 6 months after BMT, but after 1.5-2 years, rectal mucosa samples showed a decrease in the storage vacuoles of different cells. Three years after BMT, no cells with storage vacuoles were present. Allogeneic BMT slowly normalises the pathological, biochemical and MRI findings in patients with AGU.
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| 9. |
- Ihalainen, J. A., et al.
(author)
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Chromophore-Protein Interplay during the Phytochrome Photocycle Revealed by Step-Scan FTIR Spectroscopy
- 2018
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In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 140:39, s. 12396-12404
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Journal article (peer-reviewed)abstract
- Phytochrome proteins regulate many photo-responses of plants and microorganisms. Light absorption causes isomerization of the biliverdin chromophore, which triggers a series of structural changes to activate the signaling domains of the protein. However, the structural changes are elusive, and therefore the molecular mechanism of signal transduction remains poorly understood. Here, we apply twocolor step-scan infrared spectroscopy to the bacteriophytochrome from Deinococcus radiodurans. We show by recordings in H2O and D2O that the hydrogen bonds to the biliverdin D-ring carbonyl become disordered in the first intermediate (Lumi-R) forming a dynamic microenvironment, then completely detach in the second intermediate (Meta-R), and finally reform in the signaling state (Pfr). The spectra reveal via isotope labeling that the refolding of the conserved "PHY-tongue" region occurs with the last transition between Meta-R and Pfr. Additional changes in the protein backbone are detected already within microseconds in Lumi-R Aided by molecular dynamics simulations, we find that a strictly conserved salt bridge between an arginine of the PHY tongue and an aspartate of the chromophore binding domains is broken in Lumi-R and the arginine is recruited to the D-ring C=O. This rationalizes how isomerization of the chromophore is linked to the global structural rearrangement in the sensory receptor. Our findings advance the structural understanding of phytochrome photoactivation.
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- Lindwall, Magnus, 1975, et al.
(author)
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Method Effects: The Problem With Negatively Versus Positively Keyed Items
- 2012
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In: Journal of Personality Assessment. - 0022-3891. ; 94:2, s. 196-204
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Journal article (peer-reviewed)abstract
- Using confirmatory factor analyses, we examined method effects on Rosenberg's Self-Esteem Scale (RSES; Rosenberg, 1965) in a sample of older European adults. Nine hundred forty nine community-dwelling adults 60 years of age or older from 5 European countries completed the RSES as well as measures of depression and life satisfaction. The 2 models that had an acceptable fit with the data included method effects. The method effects were associated with both positively and negatively worded items. Method effects models were invariant across gender and age, but not across countries. Both depression and life satisfaction predicted method effects. Individuals with higher depression scores and lower life satisfaction scores were more likely to endorse negatively phrased items.
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| 11. |
- Liukkonen, J, et al.
(author)
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PREDICTORS OF CHANGE IN DISEASE ACTIVITY IN RHEUMATOID ARTHRITIS AFTER START OF TREATMENT WITH ABATACEPT
- 2021
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In: ANNALS OF THE RHEUMATIC DISEASES. - : BMJ. - 0003-4967 .- 1468-2060. ; 80, s. 552-553
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Conference paper (other academic/artistic)abstract
- Abatacept is a biologic disease-modifying anti-rheumatic drug (bDMARD) used to treat rheumatoid arthritis (RA) since 2006, acting by inhibition of T-cell co-stimulation. There are limited data on factors predicting clinical outcomes in RA after start of treatment with abatacept.Objectives:The primary aim was to identify predictors of change in disease activity in RA-patients after start of treatment with abatacept.Methods:This cohort study was based on data from the Swedish Rheumatology Quality register (SRQ). All patients with RA who started treatment with abatacept between 2006 and 2017 were included. Clinical data from the SRQ included demographics, disease characteristics and antirheumatic treatment. Disease activity was measured according to DAS28-ESR (Disease Activity Score of 28 joints based on erythrocyte sedimentation rate) at inclusion and at follow-up visits at 6 and 12 months from start of treatment with abatacept. Baseline predictors of change in disease activity were investigated using linear regression models bivariately and adjusted for baseline values of DAS28. Covariates with a p-value of <0.1 were retained for the final multivariate model. In case of covariates with major collinearity, the one with the stronger association with change of DAS28 was selected.Results:In a total of 2716 patients, 872 had data on change in DAS28 at 12 months. Among these, most patients were women (79.6%) and the mean age at start of abatacept was 58.4 years (SD 13.6). The majority of patients had established RA, with a mean disease duration of 13.5 years (SD 11.1). Most patients had severe, active disease, with substantial pain and disability, despite extensive treatment. The mean number of bDMARDs that a patient had been exposed to was 1.90 (SD 1.32). DAS28 decreased significantly over the first year (mean 1.22: 95 % CI 1.12, 1.32). The greatest decrease in DAS28 (mean 1.09) occurred during the first 6 months from start of abatacept. The multivariate regression model identified male sex and limited previous bDMARD exposure as independent predictors of change in DAS28 at 12 months from start of abatacept – adjusted for baseline DAS28, RA duration and current treatment with methotrexate or prednisolone (Table 1).Table 1.Determinants for retransitioningBivariateAdjusted for baseline DAS28MultivariateVariablesB95% CIP-valueB95% CIP-valueB95% CIP-valueMale sex0.39[0.13, 0.64]0.0030.43[0.20, 0.66]<0.0010.42[0.19, 0.64]<0.001Age (per SD)0.10[-0.004, 0.21]0.0580.044[-0.051, 0.14]0.37N/ARA duration (per SD)-0.14[-0.25, -0.037]0.008-0.11[-0.21, -0.016]0.022-0.030[-0.13, 0.065]0.54HAQ (per SD)0.12[0.007, 0.23]0.037-0.24[-0.35, -0.13]<0.001N/AVAS pain (per SD)0.26[0.15, 0.37]<0.001-0.058[-0.17, 0.054]0.31MTX10.20[-0.013, 0.41]0.0660.17[-0.018, 0.36]0.0760.11[-0.076, 0.29]0.25Prednisolone1-0.19[-0.41, 0.017]0.072-0.17[-0.36, 0.024]0.087-0.14[-0.33, 0.043]0.13bDMARDs2 (per SD)-0.30[-0.40, -0.20]<0.001-0.32[-0.41, -0.23]<0.001-0.31[-0.40, -0.21]<0.001B=beta coefficient; N/A=not applicable; SD=standard deviation; CI=confidence interval. Bold text indicates significant associations.DAS28, Disease activity Score of 28 joints; RA, rheumatoid arthritis; HAQ, Health Assessment Questionnaire; VAS, visual analogue scale; MTX, methotrexate; bDMARD, biologic disease-modifying antirheumatic drug.1Current treatment2Number of previous bDMARDsConclusion:In this national register study, male sex and limited previous exposure to bDMARDs were independent predictors of reduction of disease activity one year after start of treatment with abatacept for RA, possibly reflecting a better prognosis overall in such patients.Disclosure of Interests:Julia Liukkonen: None declared, Giovanni Cagnotto: None declared, Jan-Åke Nilsson: None declared, Saedis Saevarsdottir: None declared, Carl Turesson Speakers bureau: Abbvie, Bristol Myers-Squibb, Medac, Pfizer, Roche., Consultant of: Roche, Grant/research support from: This study was supported by an unrestricted grant from Bristol-Myers Squibb
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| 12. |
- Thogersen-Ntoumani, C, et al.
(author)
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Health and well-being profiles of older European adults
- 2011
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In: EUROPEAN JOURNAL OF AGEING. - : Springer Science and Business Media LLC. - 1613-9372 .- 1613-9380. ; 8:2, s. 75-85
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Journal article (peer-reviewed)abstract
- The purpose of the present study was to identify health and well-being typologies among a sample of older European adults. Further, we examined various demographic, social, and health behaviour characteristics that were used to discriminate between such groups. The participants were 1,381 community-dwelling adults aged 65 years and above (M age = 73.65; SD = 7.77) from six European Union (EU) countries who completed self-reported questionnaires. Hierarchical cluster analysis was initially conducted followed by a k means analysis to confirm cluster membership. Four clusters were identified and validated: ‘good health and moderate functioning’ (38.40%), ‘moderate health and functioning’ (30.84%), ‘obese and depressed’ (20.24%) and ‘low health and functioning’ (10.51%). The groups could be discriminated based on age, gender, nationality, years of education, social isolation and health behaviours (alcohol consumption and walking behaviour). The results of the study demonstrate heterogeneity with regard to the relationships between the variables examined. The information can be used in targeting older Europeans for health promotion interventions.
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