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Träfflista för sökning "WFRF:(Taraboulos A) "

Search: WFRF:(Taraboulos A)

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2.
  • Rouvinski, A, et al. (author)
  • Live imaging of prions reveals nascent PrPSc in cell-surface, raft-associated amyloid strings and webs
  • 2014
  • In: The Journal of cell biology. - : Rockefeller University Press. - 1540-8140 .- 0021-9525. ; 204:3, s. 423-441
  • Journal article (peer-reviewed)abstract
    • Mammalian prions refold host glycosylphosphatidylinositol-anchored PrPC into β-sheet–rich PrPSc. PrPSc is rapidly truncated into a C-terminal PrP27-30 core that is stable for days in endolysosomes. The nature of cell-associated prions, their attachment to membranes and rafts, and their subcellular locations are poorly understood; live prion visualization has not previously been achieved. A key obstacle has been the inaccessibility of PrP27-30 epitopes. We overcame this hurdle by focusing on nascent full-length PrPSc rather than on its truncated PrP27-30 product. We show that N-terminal PrPSc epitopes are exposed in their physiological context and visualize, for the first time, PrPSc in living cells. PrPSc resides for hours in unexpected cell-surface, slow moving strings and webs, sheltered from endocytosis. Prion strings observed by light and scanning electron microscopy were thin, micrometer-long structures. They were firmly cell associated, resisted phosphatidylinositol-specific phospholipase C, aligned with raft markers, fluoresced with thioflavin, and were rapidly abolished by anti-prion glycans. Prion strings and webs are the first demonstration of membrane-anchored PrPSc amyloids.
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3.
  • Luhr, KM, et al. (author)
  • Prion adsorption to stainless steel is promoted by nickel and molybdenum
  • 2009
  • In: The Journal of general virology. - : Microbiology Society. - 1465-2099 .- 0022-1317. ; 90:Pt 11, s. 2821-2828
  • Journal article (peer-reviewed)abstract
    • Prions are infectious agents resulting from the conversion of a normal cellular protein, PrPC, to a misfolded species, PrPSc. Iatrogenic transmission of prions is known from surgical procedures involving stainless steel materials. Here, it was shown that stainless steel containing nickel and molybdenum binds PrPSc more efficiently and transmits infection to cells in culture to a higher degree than if these elements are not present. Furthermore, both nickel and molybdenum alone adsorbed PrPSc, and nickel powder could be used to extract PrPSc from dilute solutions, thus providing a simple approach to concentration of PrPSc. The fact that nickel and molybdenum in steel alloys increased the binding affinity, and bound infectivity, of PrPSc is an important issue to consider in the manufacture of surgical instruments and abattoir tools.
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