| 1. |
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| 2. |
- Birney, Ewan, et al.
(author)
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Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
- 2007
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 447:7146, s. 799-816
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Journal article (peer-reviewed)abstract
- We report the generation and analysis of functional data from multiple, diverse experiments performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE Project. These data have been further integrated and augmented by a number of evolutionary and computational analyses. Together, our results advance the collective knowledge about human genome function in several major areas. First, our studies provide convincing evidence that the genome is pervasively transcribed, such that the majority of its bases can be found in primary transcripts, including non-protein-coding transcripts, and those that extensively overlap one another. Second, systematic examination of transcriptional regulation has yielded new understanding about transcription start sites, including their relationship to specific regulatory sequences and features of chromatin accessibility and histone modification. Third, a more sophisticated view of chromatin structure has emerged, including its inter-relationship with DNA replication and transcriptional regulation. Finally, integration of these new sources of information, in particular with respect to mammalian evolution based on inter- and intra-species sequence comparisons, has yielded new mechanistic and evolutionary insights concerning the functional landscape of the human genome. Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function.
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| 3. |
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| 4. |
- Huang, Hongyun, et al.
(author)
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Clinical Cell Therapy Guidelines for Neurorestoration (IANR/CANR 2017)
- 2018
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In: Cell Transplantation. - : SAGE Publications. - 0963-6897 .- 1555-3892. ; 27:2, s. 310-324
-
Research review (peer-reviewed)abstract
- Cell therapy has been shown to be a key clinical therapeutic option for central nervous system diseases or damage. Standardization of clinical cell therapy procedures is an important task for professional associations devoted to cell therapy. The Chinese Branch of the International Association of Neurorestoratology (IANR) completed the first set of guidelines governing the clinical application of neurorestoration in 2011. The IANR and the Chinese Association of Neurorestoratology (CANR) collaborated to propose the current version "Clinical Cell Therapy Guidelines for Neurorestoration (IANR/CANR 2017)". The IANR council board members and CANR committee members approved this proposal on September 1, 2016, and recommend it to clinical practitioners of cellular therapy. These guidelines include items of cell type nomenclature, cell quality control, minimal suggested cell doses, patient-informed consent, indications for undergoing cell therapy, contraindications for undergoing cell therapy, documentation of procedure and therapy, safety evaluation, efficacy evaluation, policy of repeated treatments, do not charge patients for unproven therapies, basic principles of cell therapy, and publishing responsibility.
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| 5. |
- Xiong, Jiantuan, et al.
(author)
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Hypermethylation of endoplasmic reticulum disulfide oxidase 1α leads to trophoblast cell apoptosis through endoplasmic reticulum stress in preeclampsia
- 2018
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In: Journal of Cellular Biochemistry. - : Wiley. - 0730-2312 .- 1097-4644. ; 119:10, s. 8588-8599
-
Journal article (peer-reviewed)abstract
- Abnormal trophoblast cell apoptosis is implicated in the pathogenesis of pregnancy-related disorders including preeclampsia (PE), and endoplasmic reticulum (ER) stress has been considered as a novel pathway in the regulation of cell apoptosis. In this study, we observed that both apoptosis and ER stress are triggered in trophoblast cells under hypoxia as well as in the placenta of PE rats. Quantitative polymerase chain reaction and Western blot analysis showed that the expression of endoplasmic reticulum disulfide oxidase 1α (ERO1α) is suppressed in trophoblast cells under hypoxia due to the hypermethylation of the ERO1α promoter region, and the inhibition of ERO1α expression plays an important role in ER stress and trophoblast cell apoptosis. Furthermore, we found that DNA methyltransferase 1 (DNMT1) is a key methyltransferase for DNA methylation in the regulation of ERO1α expression, and the binding level of DNMT1 to the ERO1α promoter is markedly elevated under hypoxia although DNMT1 expression is inhibited by hypoxia, suggesting that the binding level of DNMT1 to the ERO1α promoter region rather than the DNMT1 expression level contributes to the hypermethylation of ERO1α. Taken together, these results demonstrate that the hypermethylation of ERO1α mediated by increased binding of DNMT1 to the ERO1α promoter leads to trophoblast cell apoptosis through ER stress in the placenta of PE rats, which shed insight into the etiology of PE and might present a validated therapeutic target for the treatment of PE.
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| 6. |
- Xue, Ru, et al.
(author)
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Variations of methane fluxes and methane microbial community composition with soil depth in the riparian buffer zone of a sponge city park
- 2023
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In: Journal of Environmental Management. - : Elsevier. - 0301-4797 .- 1095-8630. ; 339
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Journal article (peer-reviewed)abstract
- Riparian buffers benefit both natural and man-made ecosystems by preventing soil erosion, retaining soil nu-trients, and filtering pollutants. Nevertheless, the relationship between vertical methane fluxes, soil carbon, and methane microbial communities in riparian buffers remains unclear. This study examined vertical methane fluxes, soil carbon, and methane microbial communities in three different soil depths (0-5 cm, 5-10 cm, and 10-15 cm) within a riparian buffer of a Sponge City Park for one year. Structural equation model (SEM) results demonstrated that vertical methane fluxes varied with soil depths (lambda =-0.37) and were primarily regulated by methanogenic community structure (lambda = 0.78). Notably, mathematical regression results proposed that mcrA/ pmoA ratio (R2 = 0.8) and methanogenic alpha diversity/methanotrophic alpha diversity ratio (R2 = 0.8) could serve as valid predictors of vertical variation in methane fluxes in the riparian buffer of urban river. These findings suggest that vertical variation of methane fluxes in riparian buffer soils is mainly influenced by carbon inputs and methane microbial abundance and community diversity. The study's results quantitatively the relationship between methane fluxes in riparian buffer soils and abiotic and biotic factors in the vertical di-rection, therefore contributing to the further development of mathematical models of soil methane emissions.
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| 7. |
- Yang, Anning, et al.
(author)
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Homocysteine accelerates hepatocyte autophagy by upregulating TFEB via DNMT3b-mediated DNA hypomethylation
- 2023
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In: Acta Biochimica et Biophysica Sinica. - : China Science Publishing & Media Ltd.. - 1672-9145. ; 55:8, s. 1184-1192
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Journal article (peer-reviewed)abstract
- Autophagy plays a critical role in the physiology and pathophysiology of hepatocytes. High level of homocysteine (Hcy) promotes autophagy in hepatocytes, but the underlying mechanism is still unknown. Here, we investigate the relationship between Hcy-induced autophagy level and the expression of nuclear transcription factor EB (TFEB). The results show that Hcy-induced autophagy level is mediated by upregulation of TFEB. Silencing of TFEB decreases the level of autophagy-related protein LC3BII/I and increases p62 expression level in hepatocytes after exposure to Hcy. Moreover, the effect of Hcy on the expression of TFEB is regulated by hypomethylation of the TFEB promoter catalyzed by DNA methyltransferase 3b (DNMT3b). In summary, this study shows that Hcy can activate autophagy by inhibiting DNMT3b-mediated DNA methylation and upregulating TFEB expression. These findings provide another new mechanism for Hcy-induced autophagy in hepatocytes.
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| 8. |
- Arias, Carolina, et al.
(author)
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Nuclear proteome analysis of Chlamydomonas with response to CO2 limitation
- 2020
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In: Algal Research. - : Elsevier. - 2211-9264. ; 46
-
Journal article (peer-reviewed)abstract
- Chlamydomonas reinhardtii is a unicellular green alga that can survive at a wide range of inorganic carbon (Ci) concentrations by regulating the activity of a CO2-concentrating mechanism (CCM) as well as other cellular functions. Under CO2 limited conditions, C. reinhardtii cells display a wide range of adaptive responses including changes in photosynthetic electron transport, mitochondria localization in the cells, the structure of the pyrenoid starch sheath, and primary metabolism. In addition to these functional and structural changes, gene and protein expression are also affected. Several physiological aspects of the CO2 response mechanism have been studied in detail. However, the regulatory components (transcription factors and transcriptional regulators) involved in this process are not fully characterized. Here we report a comprehensive analysis of the C. reinhardtii nuclear proteome using liquid chromatography electrospray ionization spectrometry (LC-ESI-MS). The study aims to identify the proteins that govern adaptation to varying CO2 concentrations in Chlamydomonas. The nuclear proteome of C. reinhardtii cells grown in the air at high (5%) and low (0.04%) CO2 concentrations were analyzed. Using this approach, we identified 1378 proteins in total, including 90 putative transcription factors and 27 transcriptional regulators. Characterization of these new regulatory components could shed light on the molecular mechanisms underlying acclimation to CO2 stress.
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| 9. |
- Cao, Xiaohua, et al.
(author)
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Nanoscale indentation behavior of pseudo-elastic Ti-Ni thin films
- 2008
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In: Journal of Alloys and Compounds. - : Elsevier BV. - 0925-8388 .- 1873-4669. ; 465:1-2, s. 491-496
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Journal article (peer-reviewed)abstract
- 48.28 at.%Ti-51.72 at.%Ni thin films were prepared by magnetron sputtering and post-annealed at 450, 500, 550 and 600 degrees C, respectively. The evolution of structure, phase transformation and nanoscale indentation behavior of Ti-Ni thin film annealed at different temperature were investigated by X-ray diffractometer (XRD), differential scanning calorimetry (DSC) and nanoindention test, respectively. The results showed that the as-deposited Ti-Ni thin films were amorphous and crystallized after post-annealing. As the annealing temperature increased from 450 to 600 degrees C, both the content of parent phase (B2) and that of the precipitate phase (Ni4Ti3) increased. Both the phase transformation temperature and the micro-hardness of the annealed Ti-Ni thin films increased as well. Meanwhile, the pseudo-elasticity energy recovery ratio 77 first increased to the maximum value and then decreased with the increasing annealing temperature. It revealed that the annealed Ti-Ni thin film specimens exhibited the highest pseudo-elasticity degree with the largest 71 values under the load of 10 mN.
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| 10. |
- de Seymour, Jamie V., et al.
(author)
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Metabolomic profiling of maternal hair suggests rapid development of intrahepatic cholestasis of pregnancy
- 2018
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In: Metabolomics. - : SPRINGER. - 1573-3882 .- 1573-3890. ; 14:6
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Journal article (peer-reviewed)abstract
- Intrahepatic cholestasis of pregnancy (ICP) is a common maternal liver disease; development can result in devastating consequences, including sudden fetal death and stillbirth. Currently, recognition of ICP only occurs following onset of clinical symptoms. Investigate the maternal hair metabolome for predictive biomarkers of ICP. The maternal hair metabolome (gestational age of sampling between 17 and 41 weeks) of 38 Chinese women with ICP and 46 pregnant controls was analysed using gas chromatography-mass spectrometry. Of 105 metabolites detected in hair, none were significantly associated with ICP. Hair samples represent accumulative environmental exposure over time. Samples collected at the onset of ICP did not reveal any metabolic shifts, suggesting rapid development of the disease.
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| 11. |
- Gao, Yulong, et al.
(author)
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The molecular gas resolved by ALMA in the low-metallicity merging dwarf galaxy Haro 11
- 2022
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In: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 661
-
Journal article (peer-reviewed)abstract
- Context. The physical mechanisms driving starbursts and quenching in less massive (M* ≤ 1010 M⊙) galaxies are unclear. The merger is one of the inescapable processes referred to as both starburst and quenching in massive galaxies. However, the effects of the merger on star formation in dwarf galaxies and their evolution are still uncertain.Aims. We aim to explore how star formation in dwarf galaxies is both triggered and quenched by studying metal-poor gas-rich dwarf mergers based on multi-band observations at a spatial resolution of ∼460 pc.Methods. We use archival data of Atacama Large Millimetre Array (Band 3 and 8) and Very Large Telescope/Multi Unit Spectroscopic Explorer to map CO(J = 1–0), [CI](3P1–3P0), and Hα emission in one of the most extreme merging starburst dwarf galaxies, Haro 11.Results. We find the molecular gas is assembled around the central two star-forming regions (knots B and C). The molecular and ionized gas and stellar components show complex kinematics, indicating that the gas is probably at a combined stage of collision of clouds and feedback from star formation. The peak location and distribution of [CI](1–0) closely coincide with the CO(1–0) emission, meaning that it might trace the same molecular gas as CO in such a dwarf merger starburst galaxy. The enhancement of line ratios (∼0.5) of [CI]/CO around knot C is probably generated by the dissociation of CO molecules by cosmic rays and far-ultraviolet photons. Globally, Haro 11 and its star-forming regions share similar star formation efficiency (SFE) to the high-z starburst galaxies or the clumps in nearby ultraluminous infrared galaxies.Conclusions. Given the high SFE, the high specific star formation rate, small stellar mass, low metallicity, and deficient HI gas, Haro 11 could be an analog of a high-z dwarf starburst and the potential progenitor of the nearby less massive elliptical galaxies. The significantly smaller turbulent pressure and viral parameter is probably triggering the intense starbursts. We predict that Haro 11 will quench at M* ≤ 8.5 × 109 M⊙.
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| 12. |
- Huang, Hongyun, et al.
(author)
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The 2022 yearbook of neurorestoratology
- 2023
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In: JOURNAL OF NEURORESTORATOLOGY. - : Tsinghua University Press. - 2324-2426. ; 11:2
-
Research review (peer-reviewed)abstract
- There was much progress in the field of Neurorestoratology in the year of 2022. It included highlighting advances in understanding the pathogenesis of neurological diseases, neurorestorative mechanisms, and clinical treatments as compiled in the 2022 yearbook of Neurorestoratology. There is still controversy about whether amyloid b-protein and tau protein deposition are the reasons for or the results of Alzheimer's disease (AD) pathology. The fabricated images in important key articles that speculated on the reasons for AD pathogenesis were found. Cholinergic deficiency and decrease or loss in strength of glutamatergic synapse, limited or failing bidirectional cholinergic upregulation in early cognitive impairment, or progressive posterior-to-anterior cortical cholinergic denervation could result in the appearance of AD. Exploration of neurorestorative mechanisms were found in more detail ways in neuromodulation, immunomodulation, neurogenesis, neural network or circuitry reconstruction, neuroprotection, nervous structural repair, and neuroplasticity. Several kinds of cell therapies for neurological diseases showed neurorestorative effects in open-label and/or non-randomized clinical studies or trials. However, mesenchymal stromal cells and mononuclear cells did not demonstrate neurorestorative effects or improve the quality of life for patients with neurodegenerative diseases or neurotrauma including stroke, spinal cord injury (SCI), and amyotrophic lateral sclerosis in randomized, double-blind, placebo-controlled clinical trials (RDPCTs). Clinical treatments through neurostimulation/neuromodulati on and the brain-computer/ machine interface yielded positive results in AD, Parkinson's disease, stroke, SCI, cerebral palsy, and other diseases in RDPCTs. Neurorestorative surgery, pharmaceutical neurorestorative therapy and other interventions have demonstrated neurorestorative effects for various considered incurable neurological diseases in RDPCTs. Thus, this year, additional guidelines, assessment scales, and standards were set up or revised. These included guidelines of clinical neurorestorative treatments for brain trauma (2022 China version), clinical cell therapy guidelines for neurorestoration (IANR/CANR 2022), SCI or dysfunction quality of life rating scale (SCIDQLRS) (IANR 2022 version). Neurorestorative effects of varying therapeutic stra-tegies with higher standards of evidence-based medicine are now benefiting patients with currently incurable neurological diseases. Hopefully some of them may become routine therapeutic interventions for patients with these diseases in the near future.
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| 13. |
- Lei, Wenxin, et al.
(author)
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Edge-enabled Zero Trust Architecture for ICPS with Spatial and Temporal Granularity
- 2023
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In: Proceedings - 2023 IEEE 6th International Conference on Industrial Cyber-Physical Systems, ICPS 2023. - : Institute of Electrical and Electronics Engineers (IEEE).
-
Conference paper (peer-reviewed)abstract
- Motivated by the rapid advancement of industrial cyber-physical systems (ICPS) and rising voices in favor of zero trust (ZT) security, in this paper, we present an edge-enabled zero trust architecture (ZTA) for ICPS. ZT is thought to be relevant to ICPS with the spatial and temporal granularity in the suggested architecture. In addition to continuous authentication and a dynamic access-control mechanism at the temporal granularity, we recommend edge segmentation at the spatial granularity with microservices as the division units. Finally, we conduct a security assessment of the proposed architecture in the presence of threats faced by ICPS. Overall, our analysis shows that the proposed ZTA helps to promote the security of ICPS.
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| 14. |
- Lu, Shibao, et al.
(author)
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Improving Hilbert-Huang transform for energy-correlation fluctuation in hydraulic engineering
- 2018
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In: Energy. - : Elsevier. - 0360-5442 .- 1873-6785. ; 164, s. 1341-1350
-
Journal article (peer-reviewed)abstract
- Intense vibrations in hydraulic turbine generator unit draft tubes lead to a run-out of the unit shafting and threaten its safe and stable operation. Correct maintenance is therefore important for the safe operation of such units. This study involves assessing the condition of the turbine generator unit by extracting the feature information of its vibration signals. Based on previous research, we present an enhanced Hilbert Huang transform (HHT) method with an energy-correlation fluctuation criterion to extract feature information and effectively verify the method with simulated signals. An inspection application based on the signal from a vortex strip in the draft tube of a prototype turbine under suboptimal operating conditions indicates that this method is more effective than the traditional one, with a better component identification capability and better suited to the analysis of the complex and dynamic feature information of hydro turbines.
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| 15. |
- Malhotra, Rajeev, et al.
(author)
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HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype
- 2019
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In: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 51:11, s. 1580-
-
Journal article (peer-reviewed)abstract
- Aortic calcification is an important independent predictor of future cardiovascular events. We performed a genome-wide association meta-analysis to determine SNPs associated with the extent of abdominal aortic calcification (n = 9,417) or descending thoracic aortic calcification (n = 8,422). Two genetic loci, HDAC9 and RAP1GAP, were associated with abdominal aortic calcification at a genome-wide level (P < 5.0 × 10−8). No SNPs were associated with thoracic aortic calcification at the genome-wide threshold. Increased expression of HDAC9 in human aortic smooth muscle cells promoted calcification and reduced contractility, while inhibition of HDAC9 in human aortic smooth muscle cells inhibited calcification and enhanced cell contractility. In matrix Gla protein–deficient mice, a model of human vascular calcification, mice lacking HDAC9 had a 40% reduction in aortic calcification and improved survival. This translational genomic study identifies the first genetic risk locus associated with calcification of the abdominal aorta and describes a previously unknown role for HDAC9 in the development of vascular calcification.
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| 16. |
- Ren, Bo, et al.
(author)
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Regioselective Acetylation of Diols and Polyols by Acetate Catalysis: Mechanism and Application.
- 2014
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In: Journal of Organic Chemistry. - : American Chemical Society (ACS). - 0022-3263 .- 1520-6904. ; 79:17, s. 8134-8142
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Journal article (peer-reviewed)abstract
- We propose a principle for H-bonding activation in acylation of hydroxyl groups, where the acylation is activated by the formation of hydrogen bonds between hydroxyl groups and anions. With the guidance of this principle, we demonstrate a method for the selective acylation of carbohydrates. By this method, diols and polyols are regioselectively acetylated in high yields under mild conditions using catalytic amts. of acetate. In comparison to other methods involving reagents such as organotin, organoboron, organosilicon, organobase, and metal salts, this method is more environmentally friendly, convenient, and efficient and is also assocd. with higher regioselectivity. We have performed a thorough quantum chem. study to decipher the mechanism, which suggests that acetate first forms a dual H-bond complex with a diol, which enables subsequent monoacylation by acetic anhydride under mild conditions. The regioselectivity appears to originate from the inherent structure of the diols and polyols and their specific interactions with the coordinating acetate catalyst. [on SciFinder(R)]
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| 17. |
- Song, William Wei, 1960-, et al.
(author)
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Community-based message opportunistic transmission
- 2016
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In: Transforming Healthcare Through Information Systems. - Cham : Springer International Publishing. - 9783319301327 - 9783319301334 ; , s. 79-93
-
Conference paper (peer-reviewed)abstract
- Mobile Social Networks (MSNs) is a kind of opportunistic networks, which is composed of a large number of mobile nodes with social characteristic. Up to now, the prevalent communitybased routing algorithms mostly select the most optimal social characteristic node to forward messages. But they almost don't consider the effect of community distribution on mobile nodes and the time-varying characteristic of network. These algorithms usually result in high consumption of network resources and low successful delivery ratio if they are used directly in mobile social networks. We build a time-varying community-based network model, and propose a community-aware message opportunistic transmission algorithm (CMOT) in this paper. For inter-community messages transmission, the CMOT chooses an optimal community path by comparing the community transmission probability. For intra-community in local community, messages are forwarded according to the encounter probability between nodes. The simulation results show that the CMOT improves the message successful delivery ratio and reduces network overhead obviously, compared with classical routing algorithms, such as PRoPHET, MaxProp, Spray and Wait, and CMTS.
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| 18. |
- Xu, Xiaoling, et al.
(author)
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Cable bacteria regulate sedimentary phosphorus release in freshwater sediments
- 2023
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In: Water Research. - 0043-1354 .- 1879-2448. ; 242
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Journal article (peer-reviewed)abstract
- Previous studies have demonstrated that e-SOx can regulate the sedimentary release of phosphorus (P) in brackish and marine sediments. When e-SOx is active, an iron (Fe) and manganese (Mn) oxide rich layer is formed near the sediment surface, which prevents P release. When e-SOx becomes inactive, the metal oxide layer is reduced via sulfide-mediated dissolution, and P is subsequently released to the water column. Cable bacteria have been shown to also occur in freshwater sediments. In these sediments, sulfide production is limited, and the metal oxide layer would thus dissolve less efficiently, leaving the P trapped at the sediment surface. This lack of an efficient dissolution mechanism implies that e-SOx could play an important role in the regulation of P availability in eutrophied freshwater streams. To test this hypothesis, we incubated sediments from a eutrophic freshwater river to investigate the impact of cable bacteria on sedimentary cycling of Fe, Mn and P. High-resolution depth profiling of pH, O2 and ΣH2S complemented with FISH analysis and high-throughput gene sequencing showed that the development of e-SOx activity was closely linked to the enrichment of cable bacteria in incubated sediments. Cable bacteria activity caused a strong acidification in the suboxic zone, leading to the dissolution of Fe and Mn minerals and consequently a strong release of dissolved Fe2+ and Mn2+ to the porewater. Oxidation of these mobilized ions at the sediment surface led to the formation of a metal oxide layer that trapped dissolved P, as shown by the enrichment of P-bearing metal oxides in the top layer of the sediment and low phosphate in the pore and overlying water. After e-SOx activity declined, the metal oxide layer did not dissolve and P remained trapped at the surface. Overall, our results suggested cable bacteria can play an important role to counteract eutrophication in freshwater systems.
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| 19. |
- Yang, Tianquan, et al.
(author)
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The chromosome-level genome assembly and genes involved in biosynthesis of nervonic acid of Malania oleifera
- 2023
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In: Scientific Data. - : Springer Nature. - 2052-4463. ; 10:1
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Journal article (peer-reviewed)abstract
- Nervonic acid (C24:1 Δ15, NA) is a very long-chain monounsaturated fatty acid, a clinically indispensable resource in maintaining the brain and nerve cells development and regeneration. Till now, NA has been found in 38 plant species, among which the garlic-fruit tree (Malania oleifera) has been evaluated to be the best candidate for NA production. Here, we generated a high-quality chromosome-scale assembly of M. oleifera employing PacBio long-read, short-read Illumina as well as Hi-C sequencing data. The genome assembly consisted of 1.5 Gb with a contig N50 of ~4.9 Mb and a scaffold N50 of ~112.6 Mb. ~98.2% of the assembly was anchored into 13 pseudo-chromosomes. It contains ~1123 Mb repeat sequences, and 27,638 protein-coding genes, 568 tRNAs, 230 rRNAs and 352 other non-coding RNAs. Additionally, we documented candidate genes involved in NA biosynthesis including 20 KCSs, 4 KCRs, 1 HCD and 1 ECR, and profiled the expression patterns of these genes in developing seeds. The high-quality assembly of the genome provides insights into the genome evolution of the M. oleifera genome and candidate genes involved in NA biosynthesis in the seeds of this important woody tree.
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| 20. |
- Zhang, Songhong, et al.
(author)
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Hydrophobic cryogels prepared via cryo-polymerization as oil carriers for biosynthesis of sophorolipids
- 2020
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In: Biochemical Engineering Journal. - : Elsevier BV. - 1369-703X. ; 161
-
Journal article (peer-reviewed)abstract
- Microbial synthesis of high-value biochemicals like biosurfactants using renewable substrates has attracted an intensive interest in the past decade. In this work, the bioproduction of sophorolipids was achieved by fermentation of different vegetable oils with the strain of Candida bombicola A0803 using novel hydrophobic poly(butyl methacrylate)(pBMA) cryogels as the oil substrate delivery carriers. The hydrophobic cryogels were prepared successfully via the cryo-polymerization of the reactive emulsion containing hydrophobic monomers under freezing conditions, and the characterization of cryogels was investigated experimentally. The results showed that the polymerization yields of 70 %–89 % were achieved and the cryogels had supermacropores with the maximum porosities of 92 %–96 % and good elasticity in organic solvents but rigid in water, which could provide preferable release properties for vegetable oils. Theie Young's elasticity modulus varied from 0.096 to 0.951 kPa in ethanol and acetone and the related aqueous solutions. With the pBMA cryogels as the delivery carriers of oil substrates, the maximum concentrations of sophorolipids produced for rapeseed, perilla seed and linseed oils were improved 14.5 %, 54.5 % and 53.7 %, while the maximum productivities were improved 14.5 %, 72.1 % and 42.7 % compared with those under the same fermentation condition of free carriers after 204 h–252 h of cultivation, indicating that the hydrophobic cryogels could be an interesting material for the potential applications in biosynthesis areas.
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| 21. |
- Zhang, Sheng, et al.
(author)
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Multilayer and multi-agent data fusion in WSN
- 2015
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In: Web Information Systems Engineering – WISE 2014 Workshops. - Cham : Springer International Publishing. - 9783319203706 - 9783319203690 ; , s. 211-225
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Conference paper (peer-reviewed)abstract
- In the wireless sensor networks, the hardware limitations of sensor nodes cause high transmission failure rate. We usually increase the density of nodes to improve the quality of information transmission. However, it is difficult for the limited energy supply, storage, and communication bandwidth to transfer large amount of redundant sensory data. So we use data fusion technology to remove the redundant data as much as possible before the data transmission. Data fusion becomes a research hotspot in recent years. In this paper we propose a multilayer and multi-agent data fusion mode, and analyze the proposed mode performance in three aspects: hops, energy consumption and network delay. The simulation experiments show that, if reasonably suitable parameters, such as the network scale, the number and size of agents, the data processing cost, are selected, the mobile agent mode is much better than the client/server mode.
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| 22. |
- Zhang, Xiaoling, et al.
(author)
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Developments in Fatty Acid-Derived Insect Pheromone Production Using Engineered Yeasts
- 2021
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In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 12
-
Research review (peer-reviewed)abstract
- The use of traditional chemical insecticides for pest control often leads to environmental pollution and a decrease in biodiversity. Recently, insect sex pheromones were applied for sustainable biocontrol of pests in fields, due to their limited adverse impacts on biodiversity and food safety compared to that of other conventional insecticides. However, the structures of insect pheromones are complex, and their chemical synthesis is not commercially feasible. As yeasts have been widely used for fatty acid-derived pheromone production in the past few years, using engineered yeasts may be promising and sustainable for the low-cost production of fatty acid-derived pheromones. The primary fatty acids produced by Saccharomyces cerevisiae and other yeasts are C16 and C18, and it is also possible to rewire/reprogram the metabolic flux for other fatty acids or fatty acid derivatives. This review summarizes the fatty acid biosynthetic pathway in S. cerevisiae and recent progress in yeast engineering in terms of metabolic engineering and synthetic biology strategies to produce insect pheromones. In the future, insect pheromones produced by yeasts might provide an eco-friendly pest control method in agricultural fields.
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| 23. |
- Zhang, Xiaoling, et al.
(author)
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Discovery of novel alkaline-tolerant xylanases from fecal microbiota of dairy cows
- 2023
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In: Biotechnology for Biofuels and Bioproducts. - 2731-3654. ; 16:1
-
Journal article (peer-reviewed)abstract
- Xylo-oligosaccharides (XOS) are considered as a promising type of prebiotics that can be used in foods, feeds, and healthcare products. Xylanases play a key role in the production of XOS from xylan. In this study, we conducted a metagenomic analysis of the fecal microbiota from dairy cows fed with different types of fodders. Despite the diversity in their diets, the main phyla observed in all fecal microbiota were Firmicutes and Bacteroidetes. At the genus level, one group of dairy cows that were fed probiotic fermented herbal mixture-containing fodders displayed decreased abundance of Methanobrevibacter and increased growth of beneficial Akkermansia bacteria. Additionally, this group exhibited a high microbial richness and diversity. Through our analysis, we obtained a comprehensive dataset comprising over 280,000 carbohydrate-active enzyme genes. Among these, we identified a total of 163 potential xylanase genes and subsequently expressed 34 of them in Escherichia coli. Out of the 34 expressed genes, two alkaline xylanases with excellent temperature stability and pH tolerance were obtained. Notably, CDW-xyl-8 exhibited xylanase activity of 96.1 ± 7.5 U/mg protein, with an optimal working temperature of 55 ℃ and optimal pH of 8.0. CDW-xyl-16 displayed an activity of 427.3 ± 9.1 U/mg protein with an optimal pH of 8.5 and an optimal temperature at 40 ℃. Bioinformatic analyses and structural modeling suggest that CDW-xyl-8 belongs to GH10 family xylanase, and CDW-xyl-16 is a GH11 family xylanase. Both enzymes have the ability to hydrolyze beechwood xylan and produce XOS. In conclusion, this metagenomic study provides valuable insights into the fecal microbiota composition of dairy cows fed different fodder types, revealing main microbial groups and demonstrating the abundance of xylanases. Furthermore, the characterization of two novel xylanases highlights their potential application in XOS production.
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| 24. |
- Zhao, Mingyue, et al.
(author)
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Advances in Two-Dimensional Materials for Optoelectronics Applications
- 2022
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In: Crystals. - : MDPI. - 2073-4352. ; 12:8
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Research review (peer-reviewed)abstract
- The past one and a half decades have witnessed the tremendous progress of two-dimensional (2D) crystals, including graphene, transition-metal dichalcogenides, black phosphorus, MXenes, hexagonal boron nitride, etc., in a variety of fields. The key to their success is their unique structural, electrical, mechanical and optical properties. Herein, this paper gives a comprehensive summary on the recent advances in 2D materials for optoelectronic approaches with the emphasis on the morphology and structure, optical properties, synthesis methods, as well as detailed optoelectronic applications. Additionally, the challenges and perspectives in the current development of 2D materials are also summarized and indicated. Therefore, this review can provide a reference for further explorations and innovations of 2D material-based optoelectronics devices.
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| 25. |
- Zhou, Yixuan, et al.
(author)
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H-Bonding Activation in Highly Regioselective Acetylation of Diols.
- 2013
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In: Journal of Organic Chemistry. - : American Chemical Society (ACS). - 0022-3263 .- 1520-6904. ; 78:22, s. 11618-11622
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Journal article (peer-reviewed)abstract
- H-bonding activation in the regioselective acetylation of vicinal and 1,3-diols is presented. Herein, the acetylation of the hydroxyl group with acetic anhydride can be activated by the formation of H-bonds between the hydroxyl group and anions. The reaction exhibits high regioselectivity when a catalytic amt. of tetrabutylammonium acetate is employed. Mechanistic studies indicated that acetate anion forms dual H-bonding complexes with the diol, which facilitates the subsequent regioselective monoacetylation. [on SciFinder(R)]
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