| 1. |
- Abdel Aziz, Ilaria, et al.
(author)
-
Drug delivery via a 3D electro-swellable conjugated polymer hydrogel
- 2024
-
In: Journal of materials chemistry. B. - : ROYAL SOC CHEMISTRY. - 2050-750X .- 2050-7518.
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Journal article (peer-reviewed)abstract
- Spatiotemporal controlled drug delivery minimizes side-effects and enables therapies that require specific dosing patterns. Conjugated polymers (CP) can be used for electrically controlled drug delivery; however so far, most demonstrations were limited to molecules up to 500 Da. Larger molecules could be incorporated only during the CP polymerization and thus limited to a single delivery. This work harnesses the record volume changes of a glycolated polythiophene p(g3T2) for controlled drug delivery. p(g3T2) undergoes reversible volumetric changes of up to 300% during electrochemical doping, forming pores in the nm-size range, resulting in a conducting hydrogel. p(g3T2)-coated 3D carbon sponges enable controlled loading and release of molecules spanning molecular weights of 800-6000 Da, from simple dyes up to the hormone insulin. Molecules are loaded as a combination of electrostatic interactions with the charged polymer backbone and physical entrapment in the porous matrix. Smaller molecules leak out of the polymer while larger ones could not be loaded effectively. Finally, this work shows the temporally patterned release of molecules with molecular weight of 1300 Da and multiple reloading and release cycles without affecting the on/off ratio.
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| 2. |
- Adler, Anna, et al.
(author)
-
Effect of liposome surface modification with water-soluble phospholipid polymer chain-conjugated lipids on interaction with human plasma proteins
- 2022
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 10:14, s. 2512-2522
-
Journal article (peer-reviewed)abstract
- Alternative liposome surface coatings for PEGylation to evade the immune system, particularly the complement system, have garnered significant interest. We previously reported poly(2-methacryloyloxyethyl phosphorylcholine) (MPC)-based lipids (PMPC-lipids) and investigated the surface modification of liposomes. In this study, we synthesize PMPC-lipids with polymerization degrees of 10 (MPC10-lipid), 20 (MPC20-lipid), 50 (MPC50-lipid), and 100 (MPC100-lipid), and coated liposomes with 1, 5, or 10 mol% PMPC-lipids (PMPC-liposomes). Non-modified and PEGylated liposomes are used as controls. We investigate the liposome size, surface charge, polydispersity index, and adsorption of plasma proteins to the liposomes post incubation in human plasma containing N,N,N′,N′-ethylenediamine tetraacetic acid (EDTA) or lepirudin by some methods such as sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), western blotting, and automated capillary western blot, with emphasis on the binding of complement protein C3. It is shown that the coating of liposome PMPC-lipids can suppress protein adsorption more effectively with an increase in the molecular weight and molar ratio (1-10 mol%). Apolipoprotein A-I is detected on PMPC-liposomes with a higher molecular weight and higher molar ratio of PMPC-lipids, whereas α2-macroglobulin is detected on non-modified, PEGylated, and PMPC-liposomes with a shorter polymer chain. In addition, a correlation is shown among the PMPC molecular weight, molar ratio, and C3 binding. The MPC10-lipid cannot inhibit C3 binding efficiently, whereas surface modifications with 10 mol% MPC20-lipid and 5 mol% and 10 mol% MPC50-lipid suppress both total protein and C3 binding. Hence, liposome modification with PMPC-lipids can be a possible strategy for avoiding complement activation.
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| 3. |
- Adler, Anna, et al.
(author)
-
Effect of liposome surface modification with water-soluble phospholipid polymer chain-conjugated lipids on interaction with human plasma proteins
- 2022
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 10:14, s. 2512-2522
-
Journal article (peer-reviewed)abstract
- Alternative liposome surface coatings for PEGylation to evade the immune system, particularly the complement system, have garnered significant interest. We previously reported poly(2-methacryloyloxyethyl phosphorylcholine) (MPC)-based lipids (PMPC-lipids) and investigated the surface modification of liposomes. In this study, we synthesize PMPC-lipids with polymerization degrees of 10 (MPC10-lipid), 20 (MPC20-lipid), 50 (MPC50-lipid), and 100 (MPC100-lipid), and coated liposomes with 1, 5, or 10 mol% PMPC-lipids (PMPC-liposomes). Non-modified and PEGylated liposomes are used as controls. We investigate the liposome size, surface charge, polydispersity index, and adsorption of plasma proteins to the liposomes post incubation in human plasma containing N,N,N ',N '-ethylenediamine tetraacetic acid (EDTA) or lepirudin by some methods such as sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), western blotting, and automated capillary western blot, with emphasis on the binding of complement protein C3. It is shown that the coating of liposome PMPC-lipids can suppress protein adsorption more effectively with an increase in the molecular weight and molar ratio (1-10 mol%). Apolipoprotein A-I is detected on PMPC-liposomes with a higher molecular weight and higher molar ratio of PMPC-lipids, whereas alpha(2)-macroglobulin is detected on non-modified, PEGylated, and PMPC-liposomes with a shorter polymer chain. In addition, a correlation is shown among the PMPC molecular weight, molar ratio, and C3 binding. The MPC10-lipid cannot inhibit C3 binding efficiently, whereas surface modifications with 10 mol% MPC20-lipid and 5 mol% and 10 mol% MPC50-lipid suppress both total protein and C3 binding. Hence, liposome modification with PMPC-lipids can be a possible strategy for avoiding complement activation.
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| 4. |
- Adler, Anna, et al.
(author)
-
Regulation of the innate immune system by fragmented heparin-conjugated lipids on lipid bilayered membranes in vitro
- 2023
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 11:46, s. 11121-11134
-
Journal article (peer-reviewed)abstract
- Surface modification with heparin is a powerful biomaterial coating strategy that protects against innate immunity activation since heparin is a part of the proteoglycan heparan sulfate on cell surfaces in the body. We studied the heparinization of cellular and material surfaces via lipid conjugation to a heparin-binding peptide. In the present study, we synthesized fragmented heparin (fHep)-conjugated phospholipids and studied their regulation of the innate immune system on a lipid bilayered surface using liposomes. Liposomes have versatile applications, such as drug-delivery systems, due to their ability to carry a wide range of molecules. Owing to their morphological similarity to cell membranes, they can also be used to mimic a simple cell-membrane to study protein–lipid interactions. We investigated the interaction of complement-regulators, factor H and C4b-binding protein (C4BP), as well as the coagulation inhibitor antithrombin (AT), with fHep-lipids on the liposomal surface. Herein, we studied the ability of fHep-lipids to recruit factor H, C4BP, and AT using a quartz crystal microbalance with dissipation monitoring. With dynamic light scattering, we demonstrated that liposomes could be modified with fHep-lipids and were stable up to 60 days at 4 °C. Using a capillary western blot-based method (Wes), we showed that fHep-liposomes could recruit factor H in a model system using purified proteins and assist in the degradation of the active complement protein C3b to iC3b. Furthermore, we found that fHep-liposomes could recruit factor H and AT from human plasma. Therefore, the use of fHep-lipids could be a potential coating for liposomes and cell surfaces to regulate the immune system on the lipid surface.
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| 5. |
- Adler, Anna, et al.
(author)
-
Regulation of the innate immune system by fragmented heparin-conjugated lipids on lipid bilayered membranes in vitro
- 2023
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 11:46, s. 11121-11134
-
Journal article (peer-reviewed)abstract
- Surface modification with heparin is a powerful biomaterial coating strategy that protects against innate immunity activation since heparin is a part of the proteoglycan heparan sulfate on cell surfaces in the body. We studied the heparinization of cellular and material surfaces via lipid conjugation to a heparin-binding peptide. In the present study, we synthesized fragmented heparin (fHep)-conjugated phospholipids and studied their regulation of the innate immune system on a lipid bilayered surface using liposomes. Liposomes have versatile applications, such as drug-delivery systems, due to their ability to carry a wide range of molecules. Owing to their morphological similarity to cell membranes, they can also be used to mimic a simple cell-membrane to study protein-lipid interactions. We investigated the interaction of complement-regulators, factor H and C4b-binding protein (C4BP), as well as the coagulation inhibitor antithrombin (AT), with fHep-lipids on the liposomal surface. Herein, we studied the ability of fHep-lipids to recruit factor H, C4BP, and AT using a quartz crystal microbalance with dissipation monitoring. With dynamic light scattering, we demonstrated that liposomes could be modified with fHep-lipids and were stable up to 60 days at 4 degree celsius. Using a capillary western blot-based method (Wes), we showed that fHep-liposomes could recruit factor H in a model system using purified proteins and assist in the degradation of the active complement protein C3b to iC3b. Furthermore, we found that fHep-liposomes could recruit factor H and AT from human plasma. Therefore, the use of fHep-lipids could be a potential coating for liposomes and cell surfaces to regulate the immune system on the lipid surface.
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| 6. |
- Arza, Carlos R., et al.
(author)
-
Biocompatible non-leachable antimicrobial polymers with a nonionic hyperbranched backbone and phenolic terminal units
- 2022
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 10:39, s. 8064-8074
-
Journal article (peer-reviewed)abstract
- This work aimed to develop biocompatible non-leachable antimicrobial polymers without ionic structures. A series of nonionic hyperbranched polymers (HBPs) with an isatin-based backbone and phenolic terminal units were synthesized and characterized. The molecular structures and thermal properties of the obtained HBPs were characterized by SEC, NMR, FTIR, TGA and DSC analyses. Disk diffusion assay revealed significant antibacterial activity of the obtained phenolic HBPs against nine different pathogenic bacteria. The presence of a methoxy or long alkyl group close to the phenolic unit enhanced the antibacterial effect against certain Gram positive and negative bacteria. The obtained nonionic HBPs were blended in polyester poly(hexamethylene terephthalate) films, which showed no noticeable leakage after being immersed in water for 5 days. Finally, these HBPs showed no cytotoxicity effect to MG-63 osteoblast-like human cells according to MTT analysis, and negligible hemolytic effect.
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| 7. |
- Azahar Ali, Md., et al.
(author)
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A biofunctionalized quantum dot-nickel oxide nanorod based smart platform for lipid detection
- 2016
-
In: Journal of materials chemistry. B. - : ROYAL SOC CHEMISTRY. - 2050-750X .- 2050-7518. ; 4:15, s. 2706-2714
-
Journal article (peer-reviewed)abstract
- A reagent-free, low-cost and sensitive immunosensor has been fabricated using anti-apolipoprotein B (AAB) conjugated L-cysteine in situ capped cadmium sulfide quantum dots (CysCdS QDs) bound to nickel oxide nanorods (nNiO) for detection of low density lipoprotein (LDL) molecules in human serum samples. The structural and morphological properties of AAB conjugated CysCdS QDs and nNiO have been investigated using electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and UV-visible techniques. In this immunosensor, the synthesized NiO nanorods act as mediators that allow the direct electron transfer due to their channeling effect resulting in a mediator-free biosensor. This mediator-free CysCdS-NiO based immunosensor shows improved characteristics such as a good sensitivity of 32.08 mu A (mg dl(-1))(-1) cm(-2) compared to that based on nNiO (1.42 mA (mg dl(-1))(-1) cm(-2)) alone for detection of lipid (LDL) molecules over a wide concentration range, 5-120 mg dl(-1) (0.015-0.36 mu M). The kinetic analysis yields an association constant (K-a) of 3.24 kM(-1) s(-1), indicating that the antibody conjugated CysCdS-NiO platform has a strong affinity towards lipid molecules. The excellent electron transport properties of the CysCdS-NiO nanocomposite in this immunosensor reveal that it provides an efficient platform for routine quantification of LDL molecules in real samples.
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| 8. |
- Bai, Xuan, et al.
(author)
-
Sequential macrophage transition facilitates endogenous bone regeneration induced by Zn-doped porous microcrystalline bioactive glass
- 2021
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 9:12, s. 2885-2898
-
Journal article (peer-reviewed)abstract
- Macrophages play an important role in the immune microenvironment during bone healing, and sequential macrophage phenotypic transition could achieve superior osteogenic outcomes. Microcrystalline bioactive glasses (MCBGs) with osteoimmunomodulatory effects show potential in bone tissue regeneration. Zinc (Zn) has been approved to coordinate innate and adaptive immunity. Therefore, in this study, different amounts of ZnO were incorporated into microcrystalline bioactive glass to improve its immunomodulatory ability. The effect of Zn-MCBG ionic extracts on macrophage transition was studied, and the 5Zn-MCBG extracts could orchestrate sequential M1-to-M2 macrophage transition and promote the expression of proinflammatory and anti-inflammatory genes and cytokine expression to induce human bone marrow stromal cells (hBMSCs) osteogenic differentiation in vitro. Macroporous Zn-MCBG scaffolds containing mesopores were fabricated and showed good cell adhesion and feasible apatite formation when immersed in SBF in vitro. Furthermore, a rat calvarial defect model was used to confirm that the Zn-MCBG scaffold could modulate macrophage phenotypic transition and create a desirable osteogenic microenvironment to promote osteogenesis in vivo.
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| 9. |
- Barman, Snigdha Roy, et al.
(author)
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Dendrimer as a multifunctional capping agent for metal nanoparticles for use in bioimaging, drug delivery and sensor applications
- 2018
-
In: Journal of materials chemistry. B. - : ROYAL SOC CHEMISTRY. - 2050-750X .- 2050-7518. ; 6:16, s. 2368-2384
-
Research review (peer-reviewed)abstract
- Advances in nanoparticle research, particularly in the domain of surface-engineered, function-oriented nanoparticles, have had a profound effect in many areas of scientific research and aided in bringing unprecedented developments forward, particularly in the biomedical field. Surface modifiers/capping agents have a direct bearing on the major properties of metal nanoparticles (MNPs), ranging from their physico-chemical properties to their stability and functional applications. Among the different classes of capping agents, dendrimers have gained traction as effective multifunctional capping agents for MNPs due to their unique structural qualities, dendritic effect and polydentate nature. Dendrimer-coated metal nanoparticles (DC-MNPs) are typically produced by both (i) a one-pot strategy, where metal ions are reduced in the presence of dendrimer molecules and (ii) a multi-pot strategy, where a sequence of reactions involving the reduction of metal ions, activation, conjugation and purification steps are involved. These DC-MNPs have shown remarkable ability to stabilize MNPs by means of electrostatic interactions, coordination chemistry or covalent attachment, due to them entailing a large number of sites at which further molecular moieties can be conjugated. This review article is an attempt to consolidate the on-going work, particularly over the last five years, in the field of the synthesis of dendrimer-coated MNPs and their potential applications in bioimaging, drug delivery and biochemical sensors.
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| 10. |
- Baumgartner, Johanna, et al.
(author)
-
Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells
- 2018
-
In: Journal of Materials Chemistry B. - Cambridge : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 6:28, s. 4665-4675
-
Journal article (peer-reviewed)abstract
- Hematopoietic stem cells are used in transplantations for patients with hematologic malignancies. Scarce sources require efficient strategies of expansion, including polymeric biomaterials mimicking architectures of bone marrow tissue. Tissue microenvironment and mode of cytokine presentation strongly influence cell fate. Although several cytokines with different functions as soluble or membrane-bound mediators have already been identified, their precise roles have not yet been clarified. A need exists for in vitro systems that mimic the in vivo situation to enable such studies. One way is to establish surfaces mimicking physiological presentation using protein-immobilization onto polymer films. However these films merely provide a static presentation of the immobilized proteins. It would be advantageous to also dynamically change protein presentation and functionality to better reflect the in vivo conditions. The electroactive polymer polypyrrole shows excellent biocompatibility and electrochemically alters its surface properties, becoming an interesting choice for such setups. Here, we present an in vitro system for switchable presentation of membrane-bound cytokines. We use interleukin IL-3, known to affect hematopoiesis, and show that when immobilized on polypyrrole films, IL-3 is bioavailable for the bone marrow-derived FDC-P1 progenitor cell line. Moreover, IL-3 presentation can be successfully altered by changing the redox state of the film, in turn influencing FDC-P1 cell viability. This novel in vitro system provides a valuable tool for stimuli-responsive switchable protein presentation allowing the dissection of relevant mediators in stem and progenitor cell behavior.
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| 11. |
- Bełdowski, Piotr, et al.
(author)
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Collagen type II-hyaluronan interactions - the effect of proline hydroxylation: a molecular dynamics study
- 2022
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 10:46, s. 9713-9723
-
Journal article (peer-reviewed)abstract
- Hyaluronan-collagen composites have been employed in numerous biomedical applications. Understanding the interactions between hyaluronan and collagen is particularly important in the context of joint cartilage function and the treatment of joint diseases. Many factors affect the affinity of collagen for hyaluronan. One of the important factors is the ratio of 3- or 4-hydroxy proline to proline residues. This article presents the results from molecular dynamics calculations of HA-collagen type II interactions with hyaluronan. The applied protocol employed docking and geometry optimization of complexes built using collagen structures with different numbers of hydroxyl groups attached to proline moieties. It was established that the hydroxyproline/proline ratio affects both structural and energetic features of the collagen-hyaluronan complex. Proline hydroxylation was found to significantly influence the number of all identified types of molecular forces, hydrophobic interactions, water bridges and hydrogen bonds, which can be formed between collagen and hyaluronan. Importantly, an increase in the hydroxyproline/proline ratio in the collagen chain increases the binding affinity for hyaluronan. This is illustrated by the linear correlation between the binding free energy and the hydroxylation degree. A comparison of the results obtained for 3 and 4 hydroxylation of proline indicates that the hydroxyl group attachment position plays a minor role in complex stabilization. However, a slightly stronger affinity was observed for 4 hydroxylation. In order to evaluate the effect of the aqueous environment on the collagen-hyaluronan complex stability, the enthalpic and entropic contributions to the free energy of solvation were analyzed.
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| 12. |
- Bharmoria, Pankaj, 1985, et al.
(author)
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Protein cohabitation: long-term immunoglobulin G storage at room temperature
- 2023
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 11:24, s. 5400-5405
-
Journal article (peer-reviewed)abstract
- Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.
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| 13. |
- Biswas, B., et al.
(author)
-
Near-infrared emissive cyanine probes for selective visualization of the physiological and pathophysiological modulation of albumin levels
- 2022
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518.
-
Journal article (peer-reviewed)abstract
- With the promising advantages of the near-infrared region (NIR) emissive markers for serum albumin becoming very prominent recently, we devised CyG-NHS as the cyanine derived longest NIR-I emissive optical marker possessing albumin selective recognition ability in diverse biological milieu. Multiscale modeling involving molecular docking, molecular dynamics, and implicit solvent binding free energy calculations have been employed to gain insights into the unique binding ability of the developed probe at domain-I of albumin, in contrast to the good number of domain IIA or IIIA binding probes available in the literature reports. The binding free energy was found to be −31.8 kcal mol−1 majorly predominated by hydrophobic interactions. Besides, the conformational dynamics of CyG-NHS in an aqueous medium and the albumin microenvironment have been comprehensively studied and discussed. The potentiality of this optical platform to monitor the intracellular albumin levels in human hepatoma (HepG2) cells in different pathophysiological states has been demonstrated here. Also, the competency of the phenformin drug in restoring the albumin levels in chronic hyperinsulinemic and hypercholesterolemic in vitro models has been established through the visualization approach. Altogether, the findings of this study throw light on the significance of the development of a suitable optical marker for the visualization of critical bioevents related to albumin.
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| 14. |
- Cai, Bing, et al.
(author)
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Self-setting bioceramic microscopic protrusions for transdermal drug delivery
- 2014
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In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 2:36, s. 5992-5998
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Journal article (peer-reviewed)abstract
- Microneedle (MN) technology offers both an efficient and a minimally invasive transdermal drug delivery strategy. The current MNs, made of silicon and metal, have poor biocompatibility and low drug loading, while the polymer MNs have some constraints related to mechanical strength and storage conditions. In this study, self-setting bioceramics were explored as substitutes for the current MN materials for the first time. Self-setting bioceramic microneedles were fabricated using a master mold by a procedure under mild conditions, which could minimize the drug degradation during fabrication and also facilitates a higher drug loading capability than the other current ceramic microneedles. The drug release and mechanical strength were correlated with the microstructure and porosity of the needles. As observed by SEM and microCT, the ceramic paste could fully fill the geometry of the mould and was cured into an array of micro-sized needles. The drug release study showed that the release rate from this type of MN array could be controlled by the bulk surface area, porosity and resorption rate of the ceramic needles. Applying the MNs to porcine skin indicated that the needles were able to pierce the stratum corneum of the skin. We successfully prepared the bioceramic needles that have high mechanical strength and are resorbable, which can promote safe, efficient and successful transdermal drug delivery.
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| 15. |
- Cerrato, Carmine Pasquale, et al.
(author)
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Intracellular delivery of therapeutic antisense oligonucleotides targeting mRNA coding mitochondrial proteins by cell-penetrating peptides
- 2020
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 8:47, s. 10825-10836
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Journal article (peer-reviewed)abstract
- Cell-penetrating peptides are a promising therapeutic strategy for a wide variety of degenerative diseases, ageing, and cancer. Among the multitude of cell-penetrating peptides, PepFect14 has been preferentially used in our laboratory for oligonucleotide delivery into cells and in vivo mouse models. However, this activity has mainly been reported towards cytoplasm and nuclei, while the mentioned disorders have been linked to mitochondrial defects. Here, we report a library generated from a combinatorial covalent fusion of a mitochondrial-penetrating peptide, mtCPP1, and PepFect14 in order to deliver therapeutic biomolecules to influence mitochondrial protein expression. The non-covalent complexation of these peptides with oligonucleotides resulted in nano-complexes affecting biological functions in the cytoplasm and on mitochondria. This delivery system proved to efficiently target mitochondrial genes, providing a framework for the development of mitochondrial peptide-based oligonucleotide technologies with the potential to be used as a treatment for patients with mitochondrial disorders.
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| 16. |
- Chen, Zhen, et al.
(author)
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Multifunctional conductive hydrogels and their applications as smart wearable devices
- 2021
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 9:11, s. 2561-2583
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Research review (peer-reviewed)abstract
- Recently, hydrogekbased conductive materials and their applications as smart wearable devices have been paid tremendous attention due to their high stretchability, flexibility, and excellent biocompatibility. Compared with single functional conductive hydrogels, multifunctional conductive hydrogels are more advantageous to match various demands for practical applications. This review focuses on multifunctional conductive hydrogels applied for smart wearable devices. Representative strategies for conduction of hydrogels are discussed firstly: (1) electronic conduction based on the conductive fillers and (2) ionic conduction based on charged ions. Then, the common and intensive research on multiple functionahties of conductive hydrogels, such as mechanical properties, conductive and sensory properties, anti-freezing and moisturizing properties, and adhesion and self-healing properties is presented. The applications of multifunctional conductive hydrogels such as in human motion sensors, sensory skins, and personal healthcare diagnosis are provided in the third part. Finally, we offer our perspective on open challenges and future areas of interest for multifunctional conductive hydrogels used as smart wearable devices.
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| 17. |
- Cicuendez, Monica, et al.
(author)
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Tailoring hierarchical meso- macroporous 3D scaffolds : from nano to macro
- 2014
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-750X. ; 2:1, s. 49-58
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Journal article (peer-reviewed)abstract
- Bone tissue regeneration requires the use of 3D scaffolds which mimic the architecture of the natural extracellular matrix, creating an adequate microenvironment for bone cell growth. Such 3D scaffolds need surface properties suitable for biological recognition in the early stage of cell adhesion, necessary to ensure complete cell colonization, retained cell functionality, and subsequently bone regeneration. Herein, hierarchical 3D scaffolds based on new hydroxyapatite/mesoporous glass nanocomposite bioceramic (MGHA) exhibiting different scales of porosity have been synthesized. These 3D scaffolds possess: (i) highly ordered mesopores with diameters of 10 nm; (ii) macropores with diameters in the 30-80 mu m range with interconnections of 1-10 mu m; and (iii) large macropores of ca. 500 mu m. To improve their surface properties, 3D scaffolds were modified through direct functionalization with amine propyl groups, which notably improve preosteoblast adhesion, proliferation (2.3 fold), differentiation (4.8 fold) and further cell colonization of these scaffolds. The observed enhancement can be related to these amine groups which favour early adhesion, e. g., based on nonspecific protein adsorption as was demonstrated by ellipsometry. These results suggest that the combination of hierarchical structure design and amine surface modification of hydroxyapatite/mesoporous nanocomposite scaffolds yields a double increase in cell proliferation, as well as a quadruple increase in cell differentiation, demonstrating the potential of these nanocomposite materials for bone tissue regeneration purposes.
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| 18. |
- Conte, C., et al.
(author)
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Monitoring the release of a NO photodonor from polymer nanoparticles: Via Förster resonance energy transfer and two-photon fluorescence imaging
- 2018
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 6:2, s. 249-256
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Journal article (peer-reviewed)abstract
- Core-shell polymeric nanoparticles (NPs) made of either di-block or tri-block poly-ϵ-caprolactone and polyethylene glycol copolymers, covalently integrating Rhodamine B in the core or the shell have been prepared and a green fluorescent NO photodonor entrapped therein. One- and two-photon fluorescence experiments demonstrate that effective Förster Resonance Energy Transfer (FRET) occurs exclusively in the di-block NPs having the Rhodamine in the core, accounting for a localization of the NO photoreleaser in the inner part of the polymeric nanocarrier. These di-block NPs are stable in the presence of human serum albumin and their cargo release NO under exclusive excitation with visible light. Two-photon imaging experiments carried out using 900 nm NIR light, demonstrate that the release of the NO photodonor can be monitored in biological tissue, herein human skin, and provide insights into the integrity and penetration depth of the NPs. Toxicity experiments performed on NCTC keratinocyte cell lines in the dark and upon visible light irradiation show good biocompatibility of the polymeric system that therefore has a great potential in light of the multifaceted therapeutic role of NO. © The Royal Society of Chemistry.
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| 19. |
- Craig, Marina, 1978, et al.
(author)
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Bacteria-triggered degradation of nanofilm shells for release of antimicrobial agents
- 2016
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 4:4, s. 672-682
-
Journal article (peer-reviewed)abstract
- Due to an increase in lifestyle diseases in the developed world, the number of chronic wounds is increasing at a fast pace. Chronic wound infections are common and systemic antibiotics are usually used as a treatment. In this paper we describe an approach to encapsulate antimicrobial agents in hollow microcapsules covered with a nanofilm shell that degrades through the action of a virulence factor from Pseudomonas aeruginosa. The shell was assembled using the layer-by-layer (LbL) technique with poly-l-lysine and hyaluronic acid. The microcapsules were loaded with a model substrate or a drug. By crosslinking the components in the nanofilm, the film remained intact when exposed to human wound proteases. However, the film was degraded and the drug exposed when in contact with Pseudomonas aeruginosa's Lys-X specific protease IV. The antimicrobial efficacy of the drug-loaded microcapsules was confirmed by exposure to virulent Pseudomonas aeruginosa. The current study contributes to the establishment of a release platform for targeted treatment of topical infections with the aim of minimizing both overexposure to drugs and development of bacterial resistance.
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| 20. |
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| 21. |
- Dufil, Gwennael, et al.
(author)
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Enzyme-assisted in vivo polymerisation of conjugated oligomer based conductors
- 2020
-
In: Journal of materials chemistry. B. - : ROYAL SOC CHEMISTRY. - 2050-750X .- 2050-7518. ; 8:19, s. 4221-4227
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Journal article (peer-reviewed)abstract
- Conjugated polymers conduct both electronic and ionic carriers and thus can stimulate and translate biological signals when used as active materials in bioelectronic devices. Self- and on-demand organization of the active material directly in the in vivo environment can result in the seamless integration of the bioelectronic interface. Along that line, we recently demonstrated spontaneous in vivo polymerization of the conjugated oligomer ETE-S in the vascular tissue of plants and the formation of conducting wires. In this work, we elucidate the mechanism of the in vivo polymerization of the ETE-S trimer and demonstrate that ETE-S polymerizes due to an enzymatic reaction where the enzyme peroxidase is the catalyst and hydrogen peroxide is the oxidant. ETE-S, therefore, represents the first example of a conducting polymer that is enzymatically polymerized in vivo. By reproducing the reaction in vitro, we gain further insight on the polymerization mechanism and show that hydrogen peroxide is the limiting factor. In plants the ETE-S triggers the catalytic cycle responsible for the lignification process, hacks this biochemical pathway and integrates within the plant cell wall, forming conductors along the plant structure.
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| 22. |
- Durso, M., et al.
(author)
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Biomimetic graphene for enhanced interaction with the external membrane of astrocytes
- 2018
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 6:33, s. 5335-5342
-
Journal article (peer-reviewed)abstract
- Graphene and graphene substrates display huge potential as material interfaces for devices and biomedical tools targeting the modulation or recovery of brain functionality. However, to be considered reliable neural interfaces, graphene-derived substrates should properly interact with astrocytes, favoring their growth and avoiding adverse gliotic reactions. Indeed, astrocytes are the most abundant cells in the human brain and they have a crucial physiological role to maintain its homeostasis and modulate synaptic transmission. In this work, we describe a new strategy based on the chemical modification of graphene oxide (GO) with a synthetic phospholipid (PL) to improve interaction of GO with brain astroglial cells. The PL moieties were grafted on GO sheets through polymeric brushes obtained by atom-transfer radical-polymerization (ATRP) between acryloyl-modified PL and GO nanosheets modified with a bromide initiator. The adhesion of primary rat cortical astrocytes on GO-PL substrates increased by about three times with respect to that on glass substrates coated with standard adhesion agents (i.e. poly-d-lysine, PDL) as well as with respect to that on non-functionalized GO. Moreover, we show that astrocytes seeded on GO-PL did not display significant gliotic reactivity, indicating that the material interface did not cause a detrimental inflammatory reaction when interacting with astroglial cells. Our results indicate that the reported biomimetic approach could be applied to neural prosthesis to improve cell colonization and avoid glial scar formation in brain implants. Additionally, improved adhesion could be extremely relevant in devices targeting neural cell sensing/modulation of physiological activity.
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| 23. |
- Eriksson, Viktor, 1995, et al.
(author)
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Microcapsule functionalization enables rate-determining release from cellulose nonwovens for long-term performance
- 2023
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 11:12, s. 2693-2699
-
Journal article (peer-reviewed)abstract
- Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role. Failure to sustain the release results in costs due to premature loss of functionality and resource inefficiency. Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution. In this study, microcapsules are presented as a means of extending the release from textile materials. The hydrophobic model substance pyrene is encapsulated in poly(d,l-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique. The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation. The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene. This clearly demonstrates the rate-limiting barrier properties added by the microcapsules, extending the potential functionality from hours to weeks.
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| 24. |
- Galkina, Olga, et al.
(author)
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Antibacterial and photochemical properties of cellulose nanofiber-titania nanocomposites loaded with two different types of antibiotic medicines
- 2015
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 3:35, s. 7125-7134
-
Journal article (peer-reviewed)abstract
- Nanocomposite dermal drug delivery systems based on cellulose nanofibers with grafted titania nanoparticles loaded by two antibiotic medicines from different classes, i.e. tetracycline (TC) and phosphomycin (Phos), were successfully produced by a "green chemistry'' approach in aqueous media. The influence of a different surface binding mechanism between the drug molecule and modified cellulose nanofibers on the release of the drug and, as a result, on antimicrobial properties against common pathogens Gram-positive, Staphylococcus aureus and Gram-negative Escherichia coli was investigated. The disk diffusion method and broth culture tests using varying concentrations of drugs loaded to nanocomposites were carried out to investigate the antibacterial effects. The influence of UV irradiation on the stability of the obtained nanocomposites and their antibacterial properties after irradiation were also investigated, showing enhanced stability especially for the TC loaded materials. These findings suggest that the obtained nanocomposites are promising materials for the development of potentially useful antimicrobial patches.
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| 25. |
- Galkina, Olga, et al.
(author)
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Cellulose nanofiber-titania nanocomposites as potential drug delivery systems for dermal applications
- 2015
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-750X. ; 3, s. 1688-1698
-
Journal article (peer-reviewed)abstract
- In this work, new efficient drug delivery systems based on cellulose nanofiber-titania nanocomposites grafted with three different types of model drugs such as diclofenac sodium, penicillamine-D and phosphomycin were successfully synthesized and displayed distinctly different controlled long-term release profiles. Three different methods of medicine introduction were used to show that various interactions between TiO2 and drug molecules could be used to control the kinetics of long-term drug release. All synthesis reactions were carried out in aqueous media. The morphology, chemical structure and properties of the obtained materials were characterized by SEM, TEM and AFM microscopy, nanoparticle tracking analysis, X-ray diffraction, and TGA analysis. According to FT-IR and UV-Vis spectroscopy data, the titania binds to cellulose nanofibers via formation of ester bonds and to drug molecules via formation of surface complexes. The drug release kinetics was studied in vitro for diclofenac sodium and penicillamine-D spectrophotometrically and for phosphomycin using a radio-labeling analysis with P-33-marked ATP as a model phosphate-anchored biomolecule. The results demonstrated that the obtained nanocomposites could potentially be applied in transdermal drug delivery for anesthetics, analgesics and antibiotics.
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| 26. |
- Gelmi, Amy, et al.
(author)
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Influence of conductive polymer doping on the viability of cardiac progenitor cells
- 2014
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 2:24, s. 3860-3867
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Journal article (peer-reviewed)abstract
- Cardiac tissue engineering via the use of stem cells is the future for repairing impaired heart function that results from a myocardial infarction. Developing an optimised platform to support the stem cells is vital to realising this, and through utilising new smart materials such as conductive polymers we can provide a multi-pronged approach to supporting and stimulating the stem cells via engineered surface properties, electrical, and electromechanical stimulation. Here we present a fundamental study on the viability of cardiac progenitor cells on conductive polymer surfaces, focusing on the impact of surface properties such as roughness, surface energy, and surface chemistry with variation of the polymer dopant molecules. The conductive polymer materials were shown to provide a viable support for both endothelial and cardiac progenitor cells, while the surface energy and roughness were observed to influence viability for both progenitor cell types. Characterising the interaction between the cardiac progenitor cells and the conductive polymer surface is a critical step towards optimising these materials for cardiac tissue regeneration, and this study will advance the limited knowledge on biomaterial surface interactions with cardiac cells.
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| 27. |
- Gustafsson, Hanna, 1982, et al.
(author)
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Co-immobilization of enzymes with the help of a dendronized polymer and mesoporous silica nanoparticles
- 2015
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 3:30, s. 6174-6184
-
Journal article (peer-reviewed)abstract
- The two enzymes Aspergillus sp. glucose oxidase (GOD) and horseradish peroxidase (HRP) were co-immobilized on solid silica supports in a spatially controlled way by using mesoporous silica nanoparticles (Hiroshima Mesoporous Materials, HMM) and a polycationic dendronized polymer (denpol). The silica support was first coated with the denpol, followed by the deposition of the mesoporous silica nanoparticles into which-in a next step-GOD was adsorbed. Finally, the GOD-loaded silica nanoparticles were coated with a denpol-HRP conjugate constituting of several HRP molecules which were covalently bound to the denpol via bis-aryl hydrazone (BAH) bonds. The entire immobilization process was followed in real time with quartz crystal microbalance with dissipation monitoring (QCM-D). The activities and storage stabilities of the co-immobilized enzymes were determined by analyzing a two-step cascade reaction involving the two immobilized enzymes GOD and HRP. d-glucose and o-phenylenediamine (OPD) were used as substrates for GOD and HRP, respectively. The cascade reaction-in which intermediate hydrogen peroxide was formed from d-glucose and dissolved O2 with GOD-was shown to take place. The immobilized enzymes remained fairly stable for at least 2 weeks if stored in contact with an aqueous solution of pH = 7 at 4°C. If, however, denpol-BAH-GOD coated HRP-loaded mesoporous silica nanoparticles were used (the reversed situation), the cascade reaction was not effective. This was probably due to slow diffusion of hydrogen peroxide from the surface-exposed GOD to the particle-trapped HRP, and/or due to an inefficient loading of active HRP inside the particles. Overall, the combination of two enzyme immobilization methodologies-enzymes adsorbed within mesoporous silica nanoparticles and enzymes adsorbed as denpol-BAH-enzyme conjugates-allows the spatially controlled localization of different types of enzymes in a simple way. Possible applications of the concept are in the field of bioelectrode fabrication.
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| 28. |
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| 29. |
- Han, Yi, et al.
(author)
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Highly efficient ratiometric nanothermometers based on colloidal carbon quantum dots
- 2021
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 9:20, s. 4111-4119
-
Journal article (peer-reviewed)abstract
- Optical nanothermometers have attracted much attention due to their non-contact and precise measurement with high spatial resolution at the micro- and nanoscales. They can be applied in various fields such as micro-opto-electronics, photonics, and biomedical thermal and pH sensing, while most thermal sensors reported so far contain heavy metals or have low sensitivity. Herein, we demonstrate a highly sensitive ratiometric thermal sensor based on colloidal C-dots. C-dots exhibit dual emission originating from the band gap emission and surface-dominant emission, which show a different temperature-dependent photoluminescence (PL) response. Among different surface-functionalized C-dots, C-dots@OH exhibit an absolute thermal sensitivity of -0.082 degrees C-1, which is the highest among various types of ratiometric thermosensors, making it a very promising candidate for high-sensitivity, self-calibrated nanoscale thermometry. As a proof-of-concept, C-dots@OH were employed to monitor the intracellular temperature (32-42 degrees C), showing a clear trend for temperature variation in a single cell, indicating that C-dots could offer a powerful tool for a potential precise measurement of the intracellular temperature. They could also be used as thermal sensors for nano-electronic and optoelectronic devices.
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| 30. |
- He, Wenxiao, 1985, et al.
(author)
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Morphological control of calcium phosphate nanostructures using lyotropic liquid crystals
- 2014
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 2:21, s. 3214-3220
-
Journal article (peer-reviewed)abstract
- Organisms use "soft" organic compartments to control the morphology of the embedded "hard" minerals. Here we present a simple method using liquid crystal (LC) phases as "soft" and "inert" templates to prepare nanostructured calcium phosphates (CaPs), which are inorganics of known bioefficacy. Specifically, 6 nm-thick CaP nanowires and CaP sheets that precisely replicate reverse hexagonal (H-2) and lamellar (L-alpha) LCs have been successfully synthesized and we attribute this to the sufficient spatial regulation offered by the negative (H-2) or flat curvature (L-alpha) of the aqueous domain. A normal hexagonal (H-1) phase possesses a positive curvature of the aqueous domain, therefore limited spatial restriction. For this reason, precise replication of the H-1 phase by CaP has not been possible. Interestingly, the dynamic nature of the template allowed the construction of micron-sized brushite objects with a laminated structure decorating a specific facet, possibly as a result of epitaxial overgrowth of nano-sized brushite subunits. BONA F, 1993, JOURNAL OF CRYSTAL GROWTH, V131, P331
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| 31. |
- Hed, Yvonne, et al.
(author)
-
Multipurpose heterofunctional dendritic scaffolds as crosslinkers towards functional soft hydrogels and implant adhesives in bone fracture applications
- 2013
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 1:44, s. 6015-6019
-
Journal article (peer-reviewed)abstract
- Two sets of heterofunctional dendritic frameworks displaying an inversed and exact number of ene and azide groups have successfully been synthesized and post-functionalized with biorelevant molecules. Their facile scaffolding ability enabled the fabrication of soft and azide functional dendritic hydrogels with modulus close to muscle tissue. The dendritic scaffolds are furthermore shown to be promising primers for the development of novel bone fracture stabilization adhesives with shear strengths succeeding commercial Histroacryl (R).
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| 32. |
- Hu, Zhang-Jun, et al.
(author)
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A facile "click" reaction to fabricate a FRET-based ratiometric fluorescent Cu2+ probe
- 2014
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 2:28, s. 4467-4472
-
Journal article (peer-reviewed)abstract
- A facile one-step Cu(I)-catalyzed "click" reaction, between a dansyl-azide and a propargyl-substituted rhodamine B hydrazide, is employed to fabricate a novel FRET ratiometric "off-on" fluorescent probe. The sensitive emission of the donor, a dansyl group, overlaps perfectly with the absorption of the acceptor, xanthene in the open-ring rhodamine. The proposed probe shows high selectivity towards Cu2+. The ratio of emission intensities at 568 and 540 nm (I-568/I-540) exhibits a drastic 28-fold enhancement upon addition of Cu2+. The probe shows an excellent linear relationship between emission ratios and the concentrations of Cu2+ from 10 to 50 mu M, with a detection limit (S/N = 3) of 0.12 mu M. The preliminary cellular studies demonstrated that the probe is cell membrane permeable and could be applied for ratiometric fluorescence imaging of intracellular Cu2+ with almost no cytotoxicity. The ingenuity of the probe design is to construct a FRET donor-acceptor interconnector and a selective receptor simultaneously by "click" reaction. The strategy was verified to have great potential for developing novel FRET probes for Cu2+.
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| 33. |
- Ishikawa, Mai, et al.
(author)
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Fabrication of nanocellulose-hydroxyapatite composites and their application as water-resistant transparent coatings
- 2015
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 3:28, s. 5858-5863
-
Journal article (peer-reviewed)abstract
- Nanosized composite rods similar to 300 nm in length and similar to 20 nm in width were produced by deposition of 22-77 wt% of a c-axis-oriented hydroxyapatite (HA) on cellulose nanocrystals (CNCs). The CNCs functionalized with sulphonic groups were covered with the HA nanocrystals through controlled nucleation and growth under a moderately supersaturated condition in a solution system based on a simulated body fluid. Water-resistant transparent coatings 2-4 mm thick were obtained via evaporation-induced assembly of CNC-HA nanocomposites by casting their suspension on a glass substrate and the subsequent growth of HA nanocrystals by vapour hydrothermal treatment. The composite coatings exhibited improved mechanical strength compared to that of crustacean exoskeletons, and potential for bone regeneration.
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| 34. |
- Jiang, Lingdong, et al.
(author)
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Nanohybrid polymer brushes on silica for bioseparation
- 2016
-
In: Journal of Materials Chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 4:19, s. 3247-3256
-
Journal article (peer-reviewed)abstract
- Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermogravimetric analysis (TGA). The density of polymer brushes on the surface of silica nanoparticles was determined to be 0.7 molecules per nm2. The maximum binding capacities of the nanohybrid Si@poly(NIPAm-co-GMA)@APBA for ovalbumin (OVA) and horseradish peroxidase (HRP) were determined to be 87.6 mg g-1 and 22.8 mg g-1, respectively. Glycoprotein binding on the nanohybrid could be controlled by varying the pH of the binding buffer. By increasing the temperature from 20 °C to 35 °C, glycoprotein binding onto the nanohybrid was decreased. This new pH- and temperature-responsive nanohybrid will be useful for a number of biotechnological and biomedical applications, for example, for protein separation and drug delivery.
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| 35. |
- Jin, Hua, et al.
(author)
-
Ionically interacting nanoclay and nanofibrillated cellulose lead to tough bulk nanocomposites in compression by forced self-assembly
- 2013
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 1:6, s. 835-840
-
Journal article (peer-reviewed)abstract
- Several approaches have recently been shown for self-assembled biomimetic composite films, aiming at combinations of high toughness, strength, and stiffness. However, it remains challenging to achieve high toughness using simple processes especially for bulk materials. We demonstrate that ionically interacting cationic native nanofibrillated cellulose (C-NFC) and anionic nanoclay, i.e. montmorillonite (MTM), allow local self-assemblies by a simple centrifugation process to achieve 3D bulk materials. The composite with MTM/C-NFC of 63/37 w/w has a high compressive strain to failure of 37% with distinct plastic deformation behaviour, a high work to fracture of 23.1 MJ m(-3), and a relatively high compression strength of 76 MPa. Unlike the conventionally used sequential deposition methods to achieve well-defined layers for the oppositely charged units as limited to films, the present one-step method allows quick formation of bulk materials and leads to local self-assemblies, however, having a considerable amount of nanovoids and defects between them. We suggest that the nanovoids and defects promote the plastic deformation and toughness. Considering the simple preparation method and bio-based origin of NFC, we expect that the present tough bulk nanocomposites in compression have potential in applications for sustainable and environmentally friendly materials in construction and transportation.
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| 36. |
- Kirejev, Vladimir, 1984, et al.
(author)
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A polymer-based nanodevice for the photoregulated release of NO with two-photon fluorescence reporting in skin carcinoma cells
- 2014
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 2:9, s. 1190-1195
-
Journal article (peer-reviewed)abstract
- We have developed a multifunctional biocompatible nanoconstruct based on polymeric nanoparticles encapsulating a molecular conjugate, able to photorelease nitric oxide (NO) with a fluorescent reporting function. We demonstrate that two-photon excitation (TPE) using biofriendly NIR 700 nm laser light can be applied for monitoring as well as triggering the release of NO, wherein the uncaging of a strongly fluorescent co-product acts in turn as a TPE fluorescent reporter for the simultaneous NO release from the nanoassembly. This supramolecular nanodevice internalizes in skin carcinoma cells, induces significant cell death upon light excitation and preserves its TPE properties, allowing the nearly instantaneous quantification of the NO photoreleased in cancer cells by two-photon NIR fluorescence microscopy. © 2014 The Royal Society of Chemistry.
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| 37. |
- Kislenko, Evgeniia, et al.
(author)
-
Towards molecularly imprinted polymers that respond to and capture phosphorylated tyrosine epitopes using fluorescent bis-urea and bis-imidazolium receptors.
- 2023
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 11:45, s. 10873-10882
-
Journal article (peer-reviewed)abstract
- Early detection of cancer is essential for successful treatment and improvement in patient prognosis. Deregulation of post-translational modifications (PTMs) of proteins, especially phosphorylation, is present in many types of cancer. Therefore, the development of materials for the rapid sensing of low abundant phosphorylated peptides in biological samples can be of great therapeutic value. In this work, we have synthesised fluorescent molecularly imprinted polymers (fMIPs) for the detection of the phosphorylated tyrosine epitope of ZAP70, a cancer biomarker. The polymers were grafted as nanometer-thin shells from functionalised submicron-sized silica particles using a reversible addition-fragmentation chain-transfer (RAFT) polymerisation. Employing the combination of fluorescent urea and intrinsically cationic bis-imidazolium receptor cross-linkers, we have developed fluorescent sensory particles, showing an imprinting factor (IF) of 5.0. The imprinted polymer can successfully distinguish between phosphorylated and non-phosphorylated tripeptides, reaching lower micromolar sensitivity in organic solvents and specifically capture unprotected peptide complements in a neutral buffer. Additionally, we have shown the importance of assessing the influence of counterions present in the MIP system on the imprinting process and final material performance. The potential drawbacks of using epitopes with protective groups, which can co-imprint with targeted functionality, are also discussed.
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| 38. |
- Kjellman, Tomas, et al.
(author)
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Influence of microporosity in SBA-15 on the release properties of anticancer drug dasatinib
- 2014
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 2:32, s. 5265-5271
-
Journal article (peer-reviewed)abstract
- The release of the hydrophobic cancer drug dasatinib from two mesoporous silica materials as drug delivery vehicles has been studied. One material is a reference 2D-hexagonal SBA-15 with the typical bimodal pore system with ordered primary mesopores and disordered intrawatI pores. The other material is a modified version of the same material where the intrawall porosity in the micropore regime has been selectively removed. Material characterization shows that, with the exception of the difference in intrawall porosity, the materials have identical properties. The drug dasatinib, a tyrosine kinase inhibitor, has been loaded, to the same extent, into the pores of both materials. The two materials give rise to very different release profiles of the drug. The presence of micropores leads to desired release properties: a high initial release of the drug, which is maintained over time. The lack of micropores also leads to a high initial release but followed by a rapid drop in the concentration of released drug, a consequence of its low solubility and hence crystallisation. We suggest that the presence of micropores in the carrier material, and the resultant kinetic release profile, leads to a stabilization of dasatinib in solution and to a sustained supersaturated level of the released drug. Our findings suggest that by controlling the mesoporous host with small variation in the textural properties, the kinetic release and crystallization behaviour of a drug can be altered. It is thus potentially possible to influence the drug post-release and thereby its bioavailability.
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| 39. |
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| 40. |
- Kumar, Raj, et al.
(author)
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Core-shell nanostructures : perspectives towards drug delivery applications
- 2020
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 8:39, s. 8992-9027
-
Research review (peer-reviewed)abstract
- Nanosystems have shown encouraging outcomes and substantial progress in the areas of drug delivery and biomedical applications. However, the controlled and targeted delivery of drugs or genes can be limited due to their physicochemical and functional properties. In this regard, core-shell type nanoparticles are promising nanocarrier systems for controlled and targeted drug delivery applications. These functional nanoparticles are emerging as a particular class of nanosystems because of their unique advantages, including high surface area, and easy surface modification and functionalization. Such unique advantages can facilitate the use of core-shell nanoparticles for the selective mingling of two or more different functional properties in a single nanosystem to achieve the desired physicochemical properties that are essential for effective targeted drug delivery. Several types of core-shell nanoparticles, such as metallic, magnetic, silica-based, upconversion, and carbon-based core-shell nanoparticles, have been designed and developed for drug delivery applications. Keeping the scope, demand, and challenges in view, the present review explores state-of-the-art developments and advances in core-shell nanoparticle systems, the desired structure-property relationships, newly generated properties, the effects of parameter control, surface modification, and functionalization, and, last but not least, their promising applications in the fields of drug delivery, biomedical applications, and tissue engineering. This review also supports significant future research for developing multi-core and shell-based functional nanosystems to investigate nano-therapies that are needed for advanced, precise, and personalized healthcare systems.
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| 41. |
- Kumar, Saurabh, et al.
(author)
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A solution processed carbon nanotube modified conducting paper sensor for cancer detection
- 2015
-
In: Journal of materials chemistry. B. - : ROYAL SOC CHEMISTRY. - 2050-750X .- 2050-7518. ; 3:48, s. 9305-9314
-
Journal article (peer-reviewed)abstract
- A solution processed poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS)-carbon nanotube (CNT) nano-composite has been utilized for the fabrication of a conducting paper (CP) via dip coating. Further, high conductivity of this paper (from similar to 6.5 x 10(-4) to 2.2 x 10(-2) S cm(-1)) obtained by treating it with formic acid (CNT/FA@CP) is due to the removal of the non-conducting PSS molecules from its surface. This smart conducting platform has been used for the conjugation of the anti-carcinoembronic antigen (CEA) protein for quantitative estimation of CEA, a cancer biomarker. Transmission electron microscopy (TEM), Raman spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS) and amperometric techniques have been used to characterize the low cost, flexible and environmentally friendly conducting BSA/anti-CEA/CNT/FA@CP) paper electrode that is found to be highly sensitive (7.8 mu A ng(-1) ml cm(-2)) in the physiological range (2-15 ng ml(-1)) of CEA. The response of the paper electrode is validated using CEA concentration of serum samples of cancer patients obtained via the immunoassay technique.
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| 42. |
- Latorre-Sanchez, Alejandro, et al.
(author)
-
Active quinine-based films able to release antimicrobial compounds via melt quaternization at low temperature
- 2018
-
In: Journal of materials chemistry. B. - : ROYAL SOC CHEMISTRY. - 2050-750X .- 2050-7518. ; 6:1, s. 98-104
-
Journal article (peer-reviewed)abstract
- The fabrication of antibacterial films based on renewable materials (e.g., chitosan) has attracted significant interest in fields such as food packaging, health care and medicine. However, exploiting the antibacterial properties of cinchona alkaloids to design active nanostructured films able to release quinine-based antimicrobial compounds has not been considered previously. Herein, we develop two different routes to produce active quinine-based nanostructured cross-linked films by exploiting the multiple reactive sites of quinine and, specifically, both the nitrogen atom and the vinyl group of the quinuclidine portion of the molecule, as well as their corresponding orthogonal quaternization and thiol-ene coupling reactions. The first synthetic strategy produces stiff and brittle nanostructured quinine-based films of limited utility for practical applications. Conversely, the second approach produces active, flexible and nanostructured quinine-based films (T-g = - 14 degrees C, Young's modulus = 1.3 GPa), which are able to release antimicrobial compounds against E. coli that, remarkably, are noncytotoxic against mouse macrophage and human dermal fibroblast cells. These kinds of active cinchona alkaloid-based coatings are easy to prepare by means of simple, solvent-free, melt quaternization/spreading procedures at a relatively low temperature (120 degrees C), making this second approach one of the most facile reported procedures to date to produce active nanostructured bio-based films.
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| 43. |
- Li, YY, et al.
(author)
-
A simple strategy for the efficient design of mitochondria-targeting NIR-II phototheranostics
- 2023
-
In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 11:12, s. 2700-2705
-
Journal article (peer-reviewed)abstract
- The pursuit of phototheranostic agents with near-infrared II emission, high photothermal conversion efficiency and the robust generation of reactive oxygen species (ROS) in the aggregated state is always in high demand but remains a big challenge.
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| 44. |
- Li, Zhen, et al.
(author)
-
Single cell analysis of proliferation and movement of cancer and normal-like cells on nanowire array substrates
- 2018
-
In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 6:43, s. 7042-7049
-
Journal article (peer-reviewed)abstract
- Nanowires are presently investigated in the context of various biological and medical applications. In general, these studies are population-based, which results in sub-populations being overlooked. Here, we present a single cell analysis of cell cycle and cell movement parameters of cells seeded on nanowires using digital holographic microscopy for time-lapse imaging. MCF10A normal-like human breast epithelial cells and JIMT-1 breast cancer cells were seeded on glass, flat gallium phosphide (GaP), and on vertical GaP nanowire arrays. The cells were monitored individually using digital holographic microscopy for 48 h. The data show that cell division is affected in cells seeded on flat GaP and nanowires compared to glass, with much fewer cells dividing on the former two substrates compared to the latter. However, MCF10 cells that are dividing on glass and flat GaP substrates have similar cell cycle time, suggesting that distinct cell subpopulations are affected differently by the substrates. Altogether, the data highlight the importance of performing single cell analysis to increase our understanding of the versatility of cell behavior on different substrates, which is relevant in the design of nanowire applications.
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| 45. |
- Liu, Haiyang, et al.
(author)
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A simple Schiff base as dual-responsive fluorescent sensor for bioimaging recognition of Zn2+ and Al3+ in living cells
- 2018
-
In: Journal of materials chemistry. B. - : ROYAL SOC CHEMISTRY. - 2050-750X .- 2050-7518. ; 6:34, s. 5435-5442
-
Journal article (peer-reviewed)abstract
- A simple Schiff base fluorescent sensor (BDNOL) was synthesized from the reaction of picolinohydrazide and 4-(diethylamino)salicylaldehyde, and developed for selective detection of Al3+ and Zn2+. This non-fluorescent sensor displayed obvious fluorescence enhancement after binding to Al3+/Zn2+ ions with high sensitivity and selectivity, accompanied by obvious fluorescence emission enhancement (504 nm for Al3+ and 575 nm for Zn2+). The detection limits were found to be 8.30 x 10(-8) M for Al3+ and 1.24 x 10(-7) M for Zn2+. The binding mechanisms between BDNOL and Al3+/Zn2+ ions were supported by H-1 NMR and HR-MS analysis, and a density functional theory (DFT) study. The sensing behavior was also studied with molecular logic functions of OR, AND, and NOT gates. Furthermore, the fluorescent sensor was successfully used to recognize Al3+ and Zn2+ in living cells, suggesting that this simple biosensor has great potential in biological imaging applications.
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| 46. |
- Loffler, S, et al.
(author)
-
Organic bioelectronics in infection
- 2015
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In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 3:25, s. 4979-4992
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Journal article (peer-reviewed)abstract
- Organic bioelectronics for dynamic modulation of normal and infected tissue microenvironments, and the development towards fully automated cell culture systems.
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| 47. |
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| 48. |
- Loureiro, J. A., et al.
(author)
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Fluorinated beta-sheet breaker peptides
- 2014
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In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 2:16, s. 2259-2264
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Journal article (peer-reviewed)abstract
- The aggregation of amyloid-beta peptide (Ab) has been linked to the formation of neuritic plaques, which are pathological hallmarks of Alzheimer's disease. We synthesized peptides containing fluorinated amino acids and studied their effect on the Ab aggregation. The peptides were based on the sequence LVFFD, in which valine was substituted by either 4,4,4-trifluorovaline or 4-fluoroproline, or the phenylalanine at position 3 was replaced by 3,4,5-trifluorophenylalanine. Our results demonstrate that fluorination of the hydrophobic residue valine or phenylalanine is effective in preventing the Ab aggregation. This study opens up the possibility of using new sequences based on fluorinated amino acids to inhibit the amyloid- fibril formation.
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| 49. |
- Ma, Huiting, et al.
(author)
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Nanoparticle-supported polymer brushes for temperature-regulated glycoprotein separation : Investigation of structure-function relationship
- 2018
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In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 6:22, s. 3770-3781
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Journal article (peer-reviewed)abstract
- In this work, we synthesized a series of nanoparticle-supported boronic acid polymer brushes for affinity separation of glycoproteins. Polymer brushes were prepared by surface-initiated atom transfer radical polymerization of glycidyl methacrylate and N-isopropylacrylamide, followed by stepwise modification of the pendant as well as the end functional groups to introduce boronic acid moieties through a Cu(i)-catalyzed alkyne-azide cycloaddition reaction. We investigated the impact of the polymer structure on glycoprotein binding under different pH and temperature conditions, and established new methods that allow glycoproteins to be more easily isolated and recovered with minimal alteration in solvent composition. Our experimental results suggest that for the separation of glycoproteins, terminal boronic acids located at the end of polymer chains play the most important role. The thermo-responsibility of the new affinity adsorbents, in addition to the high capacity for glycoprotein binding (120 mg ovalbumin per g adsorbent), provides a convenient means to realize simplified bioseparation not only for glycoproteins, but also for other carbohydrate-containing biological molecules.
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| 50. |
- Maazouz, Y., et al.
(author)
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Robocasting of biomimetic hydroxyapatite scaffolds using self-setting inks
- 2014
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In: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 2:33, s. 5378-5386
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Journal article (peer-reviewed)abstract
- Low temperature self-setting ceramic inks have been scarcely investigated for solid freeform fabrication processes. This work deals with the robocasting of alpha-tricalcium phosphate/gelatine reactive slurries as a bioinspired self-setting ink for the production of biomimetic hydroxyapatite/gelatine scaffolds. A controlled and totally interconnected pore network of similar to 300 mu m was obtained after ink printing and setting, with the struts consisting of a micro/nanoporous matrix of needle-shaped calcium deficient hydroxyapatite crystals, with a high specific surface area. Gelatine was effectively retained by chemical crosslinking. The setting reaction of the ink resulted in a significant increase of both the elastic modulus and the compressive strength of the scaffolds, which were within the range of the human trabecular bone. In addition to delaying the onset of the setting reaction, thus providing enough time for printing, gelatine provided the viscoelastic properties to the strands to support their own weight, and additionally enhanced mesenchymal stem cell adhesion and proliferation on the surface of the scaffold. Altogether this new processing approach opens good perspectives for the design of hydroxyapatite scaffolds for bone tissue engineering with enhanced reactivity and resorption rate.
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