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1.	Bally, Marta, 1981, et al. (author) Lipid-Based Bioanalytical Sensors 2021 In: Handbook of Lipid Membranes Molecular, Functional, and Materials Aspects. - Boca Raton : CRC Press. - 9781466555730 ; , s. 241-270, s. 241-269 Book chapter (other academic/artistic)abstract Lipid assemblies have attracted considerable interest as components in bioanalytical sensors. They provide a native-like environment for the immobilization of membrane proteins and for the study of membrane-related processes. Liposomes are also excellent bioanalytical assay components since selected functionalities can be added to the membrane while their aqueous interior can encapsulate a variety of molecules. This chapter highlights the potential of lipid assemblies in surface-based affinity sensors. It first describes how such sensors are created, providing an overview of lipid immobilization strategies together with a summary of the major transduction techniques used to probe binding at and transport through membrane interfaces. It then reviews the implementation of lipid-based sensors in the study of membrane proteins and membrane-mediated interactions, followed by a discussion of the potential of liposomes as nanoscale labels and as nanoreactors. Finally, it illustrates how external forces can be used to manipulate membrane component for biosensing applications.

Bally, Marta, 1981, et al. (author)
Lipid-Based Bioanalytical Sensors
2021
In: Handbook of Lipid Membranes Molecular, Functional, and Materials Aspects. - Boca Raton : CRC Press. - 9781466555730 ; , s. 241-270, s. 241-269
Book chapter (other academic/artistic)abstract
- Lipid assemblies have attracted considerable interest as components in bioanalytical sensors. They provide a native-like environment for the immobilization of membrane proteins and for the study of membrane-related processes. Liposomes are also excellent bioanalytical assay components since selected functionalities can be added to the membrane while their aqueous interior can encapsulate a variety of molecules. This chapter highlights the potential of lipid assemblies in surface-based affinity sensors. It first describes how such sensors are created, providing an overview of lipid immobilization strategies together with a summary of the major transduction techniques used to probe binding at and transport through membrane interfaces. It then reviews the implementation of lipid-based sensors in the study of membrane proteins and membrane-mediated interactions, followed by a discussion of the potential of liposomes as nanoscale labels and as nanoreactors. Finally, it illustrates how external forces can be used to manipulate membrane component for biosensing applications.

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