| 1. |
- Arwin, Hans
(author)
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Adsorption of Proteins at Solid Surfaces
- 2014
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In: Ellipsometry of Functional Surfaces and Films. - Berlin, Heidelberg : Springer Berlin/Heidelberg. - 9783642401275 - 9783642401282 ; , s. 29-46
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Book chapter (peer-reviewed)abstract
- Ellipsometry has a very high thin film sensitivity and can resolve sub-nm changes in the thickness of a protein film on a solid substrates. Being a technique based on photons in and photons out it can also be applied at solid-liquid interfaces. Ellipsometry has therefore found many in situ applications on protein layer dynamics but studies of protein layer structure are also frequent. Numerous ex situ applications on detection and quantification of protein layers are found and several biosensing concepts have been proposed. In this chapter, the use of ellipsometry in the above mentioned areas is reviewed and experimental methodology including cell design is briefly discussed. The classical ellipsometric challenge to determine both thickness and refractive index of a thin film is addressed and an overview of strategies to determine surface mass density is given. Included is also a discussion about spectral representations of optical properties of a protein layer in terms of a model dielectric function concept and its use for analysis of protein layer structure.
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| 2. |
- Arwin, Hans
(author)
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TIRE and SPR-enhanced SE for adsorption processes
- 2014
-
In: Ellipsometry of Functional Organic Surface and Films. - Berlin, Heidelberg : Springer Berlin/Heidelberg. - 9783642401275 - 9783642401282 ; , s. 249-264
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Book chapter (peer-reviewed)abstract
- Ellipsometry configurations in internal reflection mode facilitate studies of adsorption processes without the light beam passing through the medium from which adsorption occurs. Monitoring of adsorption processes on surfaces in opaque media is thus possible. If the surface in addition has a thin semitransparent metal film in which surface plasmon polaritons can be excited, one can achieve very high sensitivity to small changes in surface mass density of an adsorbed biolayer. Thickness changes as small as one pm can be resolved. In this chapter the theory for Total Internal Reflection Ellipsometry (TIRE), also called surface plasmon resonance enhanced ellipsometry, will be described and instrumentation will be briefly discussed. TIRE applied in spectroscopic as well as in angle of incidence interrogation modes will be considered. Finally applications in the areas of bioadsorption processes, biosensing, gas adsorption and biolayer imaging will be reviewed.
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| 3. |
- Järrendahl, Kenneth, et al.
(author)
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Polarizing Natural Nanostructures
- 2014
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In: Ellipsometry of Functional Organic Surfaces and Films. - Berlin, Heidelberg : Springer Berlin/Heidelberg. - 9783642401275 - 9783642401282 ; , s. 155-169
-
Book chapter (peer-reviewed)abstract
- A brief description of the polarizing environment we are living in and the possibilities for some animals to detect this polarization is made. This is followed by a presentation of how animals and plants generate polarized light, usually through reflection from micro- and nanostructures. Special attention is made to scarab beetles reflecting light with a high degree of circular polarization. Finally some comments on the biological aspects of polarization are made.
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