| 1. |
- Svensson, C. E., et al.
(author)
-
Collective rotational motion in the N=Z nucleus 36Ar
- 2001
-
In: Nuclear Physics A. - 0375-9474. ; 682:1-4, s. 1-11
-
Journal article (peer-reviewed)abstract
- A superdeformed rotational band has been identified in the N = Z nucleus 36Ar, firmly linked to known low-spin states, and observed to its high-spin termination at Iπ = 16+. Lifetime measurements by the Doppler shift attenuation method establish a large low-spin deformation (β2 ≈ 0.46) and a decrease in the collectivity as the band approaches termination. Comparisons with cranked Nilsson-Strutinsky and large-scale spherical shell model calculations lead to a consistent description of the band based on a configuration in which four particles are promoted to the pf shell. With two major shells active for both protons and neutrons, yet a valence space dimension small enough to be approached from the shell model perspective, this band offers an excellent opportunity to investigate the microscopic structure of collective rotational motion in nuclei.
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| 2. |
- Svensson, C. E., et al.
(author)
-
Lifetimes of superdeformed rotational states in 36Ar
- 2001
-
In: Physical Review C - Nuclear Physics. - 0556-2813. ; 63:6, s. 613011-613015
-
Journal article (peer-reviewed)abstract
- Lifetimes have been measured in a superdeformed rotational band recently identified in the N = Z nucleus 36Ar. A large low-spin quadrupole deformation (β2=0.46±0.03) is confirmed and a decrease in the collectivity is observed as the high-spin band termination at Iπ=16+ is approached. Detailed comparisons of the experimental B(E2) values with the results of cranked Nilsson-Strutinsky and large-scale (s1/2d3/2)-pf spherical shell model calculations indicate the need for a more refined treatment of transition matrix elements close to termination in the former, and the inclusion of the complete sd-pf model space in the latter description of this highly-collective rotational band.
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| 3. |
- Svensson, C. E., et al.
(author)
-
Lifetimes of superdeformed rotational states in [Formula Presented]
- 2001
-
In: Physical Review C - Nuclear Physics. - 0556-2813. ; 63:6, s. 5-5
-
Journal article (peer-reviewed)abstract
- Lifetimes have been measured in a superdeformed rotational band recently identified in the [Formula Presented] nucleus [Formula Presented] A large low-spin quadrupole deformation [Formula Presented] is confirmed and a decrease in the collectivity is observed as the high-spin band termination at [Formula Presented] is approached. Detailed comparisons of the experimental [Formula Presented] values with the results of cranked Nilsson-Strutinsky and large-scale [Formula Presented] spherical shell model calculations indicate the need for a more refined treatment of transition matrix elements close to termination in the former, and the inclusion of the complete [Formula Presented] model space in the latter description of this highly-collective rotational band.
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| 4. |
- Svensson, C. E., et al.
(author)
-
Superdeformation in the N = z Nucleus 36Ar : Experimental, deformed mean field, and spherical shell model descriptions
- 2000
-
In: Physical Review Letters. - 0031-9007. ; 85:13, s. 2693-2696
-
Journal article (peer-reviewed)abstract
- A superdeformed rotational band has been identified in 36Ar, linked to known low-spin states, and observed to its high-spin termination at Iπ = 16+. Cranked Nilsson-Strutinsky and spherical shell model calculations assign the band to a configuration in which four pf-shell orbitals are occupied, leading to a low-spin deformation β2 ≈ 0.45. Two major shells are active for both protons and neutrons, yet the valence space remains small enough to be confronted with the shell model. This band thus provides an ideal case to study the microscopic structure of collective rotational motion.
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| 5. |
- Arcopinto, M., et al.
(author)
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Growth Hormone Deficiency Is Associated with Worse Cardiac Function, Physical Performance, and Outcome in Chronic Heart Failure: Insights from the TOSCA. GHD Study
- 2017
-
In: Plos One. - : Public Library of Science (PLoS). - 1932-6203. ; 12:1
-
Journal article (peer-reviewed)abstract
- Background Although mounting evidence supports the concept that growth hormone (GH) deficiency (GHD) affects cardiovascular function, no study has systematically investigated its prevalence and role in a large cohort of chronic heart failure (CHF) patients. Aim of this study is to assess the prevalence of GHD in mild-to-moderate CHF and to explore clinical and functional correlates of GHD. One-hundred thirty CHF patients underwent GH provocative test with GHRH+arginine and accordingly categorized into GH-deficiency (GHD, n = 88, age = 61.6 +/- 1.1 years, 68% men) and GH-sufficiency (GHS, n = 42, age = 63.6 +/- 1.5 years, 81% men) cohorts. Both groups received comprehensive cardiovascular examination and underwent Doppler echocardiography, cardiopulmonary exercise testing, and biochemical and hormonal assay. GHD was detected in roughly 30% of CHF patients. Compared to GHD, GHS patients showed smaller end-diastolic and end-systolic LV volumes (-28%, p=.008 and -24%, p=.015, respectively), lower LV end-systolic wall stress (-21%, p=.03), higher RV performance (+18% in RV area change, p=.03), lower estimated systolic pulmonary artery pressure (-11%, p=.04), higher peak VO2 (+20%, p=.001) and increased ventilatory efficiency (-12% in VE/VCO2 slope, p=.002). After adjusting for clinical covariates (age, gender, and tertiles of LV ejection fraction, IGF-1, peak VO2, VE/VCO2 slope, and NT-proBNP), logistic multivariate analysis showed that peak VO2 (beta = -1.92, SE = 1.67, p=.03), VE/VCO2 slope (beta = 2.23, SE = 1.20, p=.02) and NT-proBNP (beta = 2.48, SE = 1.02, p=.016), were significantly associated with GHD status. Finally, compared to GHS, GHD cohort showed higher all-cause mortality at median follow-up of 3.5 years (40% vs. 25%, p<.001, respectively), independent of age, sex, NT-proBNP, peak VO2 and LVEF. GH deficiency identifies a subgroup of CHF patients characterized by impaired functional capacity, LV remodeling and elevated NT-proBNP levels. GHD is also associated with increased all-cause mortality.
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| 6. |
- Moschella, P. S., et al.
(author)
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Low-crested coastal defence structures as artificial habitats for marine life: Using ecological criteria in design
- 2005
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In: Coastal Engineering. - : Elsevier BV. - 0378-3839. ; 52:10-11, s. 1053-1071
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Journal article (peer-reviewed)abstract
- Coastal defence structures to protect sedimentary coastlines from erosion and flooding are increasingly common throughout Europe. They will become more widespread over the next 10-30 years in response to rising and stormier seas and accelerating economic development of the coastal zone. Building coastal defences results in the loss and fragmentation of sedimentary habitats and their replacement by artificial rocky habitats that become colonised by algae and marine animals. The engineering design and construction of these structures have received considerable attention. However, the ecological consequences of coastal defences have been less extensively investigated. Furthermore, due to their rapid proliferation, there is a growing need to understand the role of these man-made habitats in the coastal ecosystems in order to implement impact minimisation and/or mitigation measures. As part of the DELOS project, targeted studies were carried out throughout Europe to assess the ecological similarity of low-crested coastal defence structures (LCS) to natural rocky shores and to investigate the influence of LCS design features on the colonising marine epibiota. LCSs can be considered as a relatively poor surrogate of natural rocky shores. Epibiotic communities were qualitatively similar to those on natural rocky shores as both habitats are regulated by the same physical and biological factors. However, there were quantitative differences in the diversity and abundance of epibiota on artificial structures. Typically, epibiotic assemblages were less diverse than rocky shore communities. Also, LCSs offered less structurally complex habitats for colonisation and in some locations experienced higher disturbance than natural shores. We propose several criteria that can be integrated into the design and construction of LCSs to minimise ecological impacts and allow targeted management of diversity and natural living resources. (c) 2005 Elsevier B.V. All rights reserved.
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| 7. |
- Rudolph, D., et al.
(author)
-
Onset of high-spin rotational bands in the N=Z nucleus 62Ga
- 2020
-
In: Physical Review C. - : American Physical Society. - 2469-9985. ; 102:1
-
Journal article (peer-reviewed)abstract
- The fusion-evaporation reaction 28Si + 40Ca at 122 MeV beam energy was used to populate high-spin states in the odd-odd N = Z nucleus 62Ga. With the combination of the Gammasphere spectrometer and the Microball CsI(Tl) charged-particle detector array the decay scheme of 62Ga was extended beyond 10 MeV excitation energy. The onset of band structures was observed. These high-spin rotational states are interpreted and classified by means of cranked Nilsson-Strutinsky calculations.
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| 8. |
- Seymour, M. L., et al.
(author)
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Leukotriene and prostanoid pathway enzymes in bronchial biopsies of seasonal allergic asthmatics
- 2001
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In: Am J Respir Crit Care Med. - 1073-449X. ; 164:11, s. 2051-6
-
Journal article (peer-reviewed)abstract
- Cysteinyl-leukotrienes and prostaglandin D2 generated by the 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathways, respectively, cause bronchoconstriction, leukocyte recruitment, and bronchial hyperresponsiveness in asthma. We characterized the cellular expression of 5-LO and COX enzymes using immunohistochemistry on bronchial biopsies from 12 allergic asthmatic patients before and during seasonal exposure to birch pollen. Bronchial responsiveness (p = 0.004) and symptoms (p < 0.005) increased and peak expiratory flow (PEF; p < or = 0.02) decreased in the pollen season. In-season biopsies had 2-fold more cells immunostaining for 5-LO (p = 0.02), 5-LO-activating protein (FLAP; p = 0.04), and leukotriene (LT)A4 hydrolase (p = 0.05), and 4-fold more for the terminal enzyme for cysteinyl-leukotriene synthesis, LTC4 synthase (p = 0.02). Immunostaining for COX-1, COX-2, and PGD2 synthase was unchanged. Increased staining for LTC4 synthase was due to increased eosinophils (p = 0.035) and an increased proportion of eosinophils expressing the enzyme (p = 0.047). Macrophages also increased (p = 0.019), but mast cells and T-lymphocyte subsets were unchanged. Inverse correlations between PEF and 5-LO(+) cell counts link increased expression of 5-LO pathway enzymes in eosinophils and macrophages within the bronchial mucosa to deterioration of lung function during seasonal allergen exposure.
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| 9. |
- Åberg, N David, 1970, et al.
(author)
-
Peripheral infusion of insulin-like growth factor-I increases the number of newborn oligodendrocytes in the cerebral cortex of adult hypophysectomized rats.
- 2007
-
In: Endocrinology. - : The Endocrine Society. - 0013-7227 .- 1945-7170. ; 148:8, s. 3765-72
-
Journal article (peer-reviewed)abstract
- We have previously shown that recombinant human (rh) IGF-I induces cell proliferation and neurogenesis in the hippocampus of hypophysectomized rats. In the current investigation, we determined the effects of rhIGF-I on proliferation and differentiation in the cerebral cortex. Adult hypophysectomized rats were injected with bromodeoxyuridine (BrdU) to label newborn cells (once a day for the first 5 d), and rhIGF-I was administered peripherally for 6 or 20 d. In the cerebral cortex, the number of BrdU-labeled cells increased after 20 d but not after 6 d of rhIGF-I infusion. This suggests that rhIGF-I enhances the survival of newborn cells in the cerebral cortex. Using BrdU labeling combined with the oligodendrocyte-specific markers myelin basic protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase, we demonstrated an increase in oligodendrogenesis in the cerebral cortex. The total amount of myelin basic protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase was also increased on Western blots of homogenates of the cerebral cortex, confirming the immunohistochemical findings. Also, we observed an increase in the number of capillary-associated BrdU-positive cells, although total capillary area was not increased. rhIGF-I treatment did not affect cortical astrogliogenesis and neurogenesis was not observed. The ability of rhIGF-I to induce cortical oligodendrogenesis may have implications for the regenerative potential of the cortex.
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| 10. |
- Åberg, N David, 1970, et al.
(author)
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Relationship between Levels of Pre-Stroke Physical Activity and Post-Stroke Serum Insulin-Like Growth Factor I
- 2020
-
In: Biomedicines. - : MDPI AG. - 2227-9059. ; 8:3
-
Journal article (peer-reviewed)abstract
- Physical activity (PA) and insulin-like growth factor I (IGF-I) have beneficial effects for patients who have suffered an ischemic stroke (stroke). However, the relationship between the levels of PA and IGF-I after stroke has not been explored in detail. We investigated the pre-stroke PA level in relation to the post-stroke serum IGF-I (s-IGF-I) level, at baseline and at 3 months after the index stroke, and calculated the change that occurred between these two time-points (Delta IGF-I). Patients (N = 380; 63.4% males; mean age, 54.7 years) with data on 1-year leisure-time pre-stroke PA and post-stroke s-IGF-I levels were included from the Sahlgrenska Academy Study on Ischemic Stroke (SAHLSIS). Stroke severity was assessed using the National Institutes of Health Stroke Scale (NIHSS). Pre-stroke, leisure-time PA was self-reported as PA1-4, with PA1 representing sedentary and PA2-4 indicating progressively higher PA levels. Associations between s-IGF-I and PA were evaluated by multiple linear regressions with PA1 as the reference and adjustments being made for sex, age, history of previous stroke or myocardial infarctions, cardiovascular risk factors, and stroke severity. PA correlated with baseline s-IGF-I and Delta IGF-I, but not with the 3-month s-IGF-I. In the linear regressions, there were corresponding associations that remained as a tendency (baseline s-IGF-I, p = 0.06) or as a significant effect (Delta IGF-I, p = 0.03) after all the adjustments. Specifically, for each unit of PA, Delta IGF-I increased by 9.7 (95% CI 1,1-18.4) ng/mL after full adjustment. This supports the notion that pre-stroke PA is independently related to Delta IGF-I.
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| 11. |
- Ahlström, Katarina, 1966, et al.
(author)
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Exogenous carbon monoxide does not affect cell membrane energy availability assessed by sarcolemmal calcium fluxes during myocardial ischaemia-reperfusion in the pig
- 2011
-
In: European Journal of Anaesthesiology. - 0265-0215 .- 1365-2346. ; 28:5, s. 356-362
-
Journal article (peer-reviewed)abstract
- Carbon monoxide is thought to be cytoprotective and may hold therapeutic promise for mitigating ischaemic injury. The purpose of this study was to test low-dose carbon monoxide for protective effects in a porcine model of acute myocardial ischaemia and reperfusion. In acute open-thorax experiments in anaesthetised pigs, pretreatment with low-dose carbon monoxide (5% increase in carboxyhaemoglobin) was conducted for 120 min before localised ischaemia (45 min) and reperfusion (60 min) was performed using a coronary snare. Metabolic and injury markers were collected by microdialysis sampling in the ventricular wall. Recovery of radio-marked calcium delivered locally by microperfusate was measured to assess carbon monoxide treatment effects during ischaemia/reperfusion on the intracellular calcium pool. Coronary occlusion and ischaemia/reperfusion were analysed for 16 animals (eight in each group). Changes in glucose, lactate and pyruvate from the ischaemic area were observed during ischaemia and reperfusion interventions, though there was no difference between carbon monoxide-treated and control groups during ischaemia or reperfusion. Similar results were observed for glycerol and microdialysate Ca-45(2+) recovery. These findings show that a relatively low and clinically relevant dose of carbon monoxide did not seem to provide acute protection as indicated by metabolic, energy-related and injury markers in a porcine myocardial ischaemia/reperfusion experimental model. We conclude that protective effects of carbon monoxide related to ischaemia/reperfusion either require higher doses of carbon monoxide or occur later after reperfusion than the immediate time frame studied here. More study is needed to characterise the mechanism and time frame of carbon monoxide-related cytoprotection.
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| 12. |
- Ala-Laurinaho, J., et al.
(author)
-
TUMESA - MEMS tuneable metamaterials for smart wireless applications
- 2012
-
In: European Microwave Week 2012: "Space for Microwaves", EuMW 2012, Conference Proceedings - 7th European Microwave Integrated Circuits Conference, EuMIC 2012. - : IEEE. - 9782874870286 ; , s. 95-98
-
Conference paper (peer-reviewed)abstract
- This paper describes the main results of the EU FP7 project TUMESA - MEMS tuneable metamaterials for smart wireless applications. In this project, we studied several reconfigurable antenna approaches that combine the new technology of MEMS with the new concept of artificial electromagnetic materials and surfaces (metamaterials and metasurfaces) for realisation of millimetre wave phase shifters and beam-steering devices. MEMS technology allows to miniaturise electronic components, reduce their cost in batch production, and effectively compete with semiconductor and ferroelectric based technologies in terms of losses at millimetre wavelengths. Novel tuneable materials and components proposed in this project perform as smart beam steering devices. Fabricated with MEMS technology in batch and on a single chip, proposed tuneable devices allow substituting of larger and more complex sub-system of, e.g., a radar sensor. This substitution provides a dramatic cost reduction on a system level.
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| 13. |
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| 14. |
- Anniballi, F., et al.
(author)
-
Management of animal botulism outbreaks : From clinical suspicion to practical countermeasures to prevent or minimize outbreaks
- 2013
-
In: Biosecurity and bioterrorism. - : Mary Ann Liebert Inc. - 1538-7135 .- 1557-850X. ; 11:SUPPL. 1, s. S191-S199
-
Journal article (peer-reviewed)abstract
- Botulism is a severe neuroparalytic disease that affects humans, all warm-blooded animals, and some fishes. The disease is caused by exposure to toxins produced by Clostridium botulinum and other botulinum toxin-producing clostridia. Botulism in animals represents a severe environmental and economic concern because of its high mortality rate. Moreover, meat or other products from affected animals entering the food chain may result in a public health problem. To this end, early diagnosis is crucial to define and apply appropriate veterinary public health measures. Clinical diagnosis is based on clinical findings eliminating other causes of neuromuscular disorders and on the absence of internal lesions observed during postmortem examination. Since clinical signs alone are often insufficient to make a definitive diagnosis, laboratory confirmation is required. Botulinum antitoxin administration and supportive therapies are used to treat sick animals. Once the diagnosis has been made, euthanasia is frequently advisable. Vaccine administration is subject to health authorities' permission, and it is restricted to a small number of animal species. Several measures can be adopted to prevent or minimize outbreaks. In this article we outline all phases of management of animal botulism outbreaks occurring in wet wild birds, poultry, cattle, horses, and fur farm animals. © 2013, Mary Ann Liebert, Inc.
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| 15. |
- Araujo, R., et al.
(author)
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The role of disturbance in differential regulation of co-occurring brown algae species: Interactive effects of sediment deposition, abrasion and grazing on algae recruits
- 2012
-
In: Journal of Experimental Marine Biology and Ecology. - : Elsevier BV. - 0022-0981. ; 422-423, s. 1-8
-
Journal article (peer-reviewed)abstract
- Disturbance is an important structuring factor in intertidal communities. This study aimed at experimentally testing the effects of three important sources of disturbance to intertidal rocky habitats (abrasion, sediment deposition and herbivory) on the survivorship and growth of embryos of canopy forming fucoid species (Fucus vesiculosus and Fucus serratus). Natural levels of sediment deposition and herbivore density were characterized and the temporal variation of populations of both species was followed. The experimental study showed that the survivorship of F. serratus embryos was negatively affected by herbivory but growth was enhanced in the presence of abrasion and herbivores. F. vesiculosus suffered the interactive negative effects of the three sources of disturbance tested, although abrasion was the most important factor affecting mortality rates of embryos. Conversely, none of the tested sources of disturbance significantly affected growth of embryos. These results highlight the importance of disturbance events in regulating species interactions which might be particularly relevant for population dynamics and persistence at the geographical borders of species' distribution. (C) 2012 Elsevier B.V. All rights reserved.
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| 16. |
- Araujo, R., et al.
(author)
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TRADE-OFFS BETWEEN LIFE-HISTORY TRAITS AT RANGE-EDGE AND CENTRAL LOCATIONS
- 2015
-
In: Journal of Phycology. - : Wiley. - 0022-3646. ; 51:4, s. 808-818
-
Journal article (peer-reviewed)abstract
- The allocation of resources to different life-history traits should represent the best compromise in fitness investment for organisms in their local environment. When resources are limiting, the investment in a specific trait must carry a cost that is expressed in trade-offs with other traits. In this study, the relative investment in the fitness-related traits, growth, reproduction and defence were compared at central and range-edge locations, using the seaweed Ascophyllum nodosum as a model system. Individual growth rates were similar at both sites, whereas edge populations showed a higher relative investment in reproduction (demonstrated by a higher reproductive allocation and extended reproductive periods) when compared to central populations that invested more in defence. These results show the capability of A. nodosum to differentially allocate resources for different traits under different habitat conditions, suggesting that reproduction and defence have different fitness values under the specific living conditions experienced at edge and central locations. However, ongoing climate change may threaten edge populations by increasing the selective pressure on specific traits, forcing these populations to lower the investment in other traits that are also potentially important for population fitness.
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| 17. |
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| 18. |
- Cox, D. M., et al.
(author)
-
Spectroscopy along flerovium decay chains. II. Fine structure in odd-A 289Fl
- 2023
-
In: Physical Review C. - 2469-9985. ; 107:2
-
Journal article (peer-reviewed)abstract
- Fifteen correlated α-decay chains starting from the odd-A superheavy nucleus 289Fl were observed following the fusion-evaporation reaction 48Ca+244Pu. The results call for at least two parallel α-decay sequences starting from at least two different states of 289Fl. This implies that close-lying levels in nuclei along these chains have quite different spin-parity assignments. Further, observed α-electron and α-photon coincidences, as well as the α-decay fine structure along the decay chains, suggest a change in the ground-state spin assignment between 285Cn and 281Ds. Our experimental results, on the excited level structure of the heaviest odd-N nuclei to date, provide a direct testing ground for theory. This is illustrated by comparison with new nuclear structure calculations based on the symmetry-conserving configuration mixing theory.
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| 19. |
- Dahlén, Gunnar, 1944, et al.
(author)
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Subgingival bacteria in Ghanaian adolescents with or without progression of attachment loss
- 2014
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In: Journal of Oral Microbiology. - : Informa UK Limited. - 2000-2297. ; 6:1
-
Journal article (peer-reviewed)abstract
- Objective: This study describes subgingival bacterial profiles associated with clinical periodontal status in Ghanaian adolescents with or without progression of attachment loss. Materials and methods: Among 500 adolescents included in a cohort study, 397 returned 2 years later for a periodontal re-examination, including full-mouth CAL measurements. At follow-up, a subgroup of 98 adolescents was also subjected to bacterial sampling with paper points at four periodontal sites (mesial aspect of 11, 26, 31, and 46) and analyzed with the checkerboard DNA - DNA hybridization technique against DNA-probes from nine periodontitis-associated bacterial species. Results: The 98 Ghanaian adolescents examined in the present study were similar to the entire group examined at the 2-year follow-up with respect to age, gender, and CAL > 3 mm. A high detection frequency of Fusobacterium nucleatum and Prevotella intermedia (> 99%) using checkerboard analysis was found, while for Aggregatibacter actinomycetemcomitans the detection frequency was < 50%. A strong correlation was found at the individual level between the presence of P. intermedia and the total CAL change, and P. intermedia and Porphyromonas gingivalis were strongly correlated with a change in CAL and probing pocket depth (PPD) at the sampled sites. In a linear regression model, a significant discriminating factor for the total CAL change in the dentition during the 2-year follow-up period was obtained for P. intermedia and public school. Conclusion: This study indicates that subgingival bacterial species other than A. actinomycetemcomitans, for example, P. intermedia, have a significant association with periodontal breakdown (change in CAL) in Ghanaian adolescents with progression of periodontal attachment loss.
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| 20. |
- de Sousa, Pedro F. M., 1977-
(author)
-
Chemometrics : Unravelling information from complex data generated by modern instruments
- 2021
-
Doctoral thesis (other academic/artistic)abstract
- Chemometrics is a discipline dedicated to solving problems arising from complicated analytical systems, combining statistics, mathematics, and computational programming languages.This thesis is based on the work developed in four scientific projects published as papers in scientific journals. The studies developed in these projects have been essentially focused on a data analysis perspective, interpreting complicated data by means of algorithms, employing chemometrical methodologies. Several chemometrical approaches, based on multivariate data analysis and signal processing algorithms have been studied and employed in each project. Most of the data analysis problems studied these projects are related to liquid chromatography hyphenated to mass spectrometry systems, including tandem mass spectrometry. One of the projects has been related to spectrophotometric data.Chromatographic peak shifts have been attributed to lack of control of the nominal chromatographic parameters. The purpose of the work presented in Paper I was to study retention time data, obtained experimentally by provoking peak shifts with controlled effects, to demonstrate that there are patterns associated with such changing factors affecting chromatographic processes. PCR (Principal Component Regression) models were calculated for each compound (98 compounds), using the retention time data of each compound as responses (y), and the retention time data of the remaining compounds as regressors (X). The results demonstrate that the peak shifts of each compound across samples are correlated with the peak shifts of the other compounds in the chromatographic data. This work confirmed a previous work, where an algorithm was developed to improve alignment of peaks in large number of complex samples, based on peak shift patterns.Partial Least Squares (PLS) is one of the mostly used chemometrics techniques. In the work presented in Paper II, a previously reported modified PLS algorithm was studied. This algorithm was developed with the purpose of not generating overfitting models with increasing noise in X, which happens with the classical PLS. However, the results in less-noisy data were not as good as the classical PLS. From this study, we have developed another modified algorithm that does not overfit with increasing noise in X, and it converges with the solutions of the classical PLS in less-noisy data.DNA adductomics is a recent field in omics that studies modifications in the DNA. The goal of the project in Paper III was to develop a program with a graphical interface to interpret LC-MS/MS using a data independent acquisition method, to identify adducts in DNA nucleosides. The results were compared with those performed manually. The program detected over 150 potential adducts whereas manually, in a previous work, only about 25 were found. This program can detect adducts automatically in a matter of seconds.Cancer has been associated with processes that are related to exposure to pollutants and the consumption of certain food products. This process has been related to electrophilic compounds that react with DNA (adducts). When DNA modifications occur, often defense mechanisms in the cell are triggered often leading to the rupture of the cell. Fragments of DNA (micronuclei) are then roaming in the blood stream. In this work (Paper IV), electrophilic additions to hemoglobin (adducts) and the expression of micronuclei in blood samples from 50 children were studied. One of the goals of the project was to find correlations between the adducts in hemoglobin and the expression of micronuclei. PLS was used to model the data. However, the results were not conclusive (R2 = 0.60), i.e., there may be some trends, but there are other variables not modelled that may influence the variance in expression of micronuclei.
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| 21. |
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| 22. |
- Fielding, R. A., et al.
(author)
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Effect of structured physical activity and nutritional supplementation on physical function in mobility-limited older adults : Results from the VIVE2 randomized trial
- 2017
-
In: The Journal of Nutrition, Health & Aging. - : Springer Science and Business Media LLC. - 1279-7707 .- 1760-4788. ; 21:9, s. 936-942
-
Journal article (peer-reviewed)abstract
- The interactions between nutritional supplementation and physical activity on changes in physical function among older adults remain unclear. The primary objective of this study was to examine the impact of nutritional supplementation plus structured physical activity on 400M walk capacity in mobility-limited older adults across two sites (Boston, USA and Stockholm, Sweden). All subjects participated in a physical activity program (3x/week for 24 weeks), involving walking, strength, balance, and flexibility exercises. Subjects were randomized to a daily nutritional supplement (150kcal, 20g whey protein, 800 IU vitamin D) or placebo (30kcal, non-nutritive). Participants were recruited from urban communities at 2 field centers in Boston MA USA and Stockholm SWE. Mobility-limited (Short Physical Performance Battery (SPPB) ae9) and vitamin D insufficient (serum 25(OH) D 9 - 24 ng/ml) older adults were recruited for this study. Primary outcome was gait speed assessed by the 400M walk. Results: 149 subjects were randomized into the study (mean age=77.5 +/- 5.4; female=46.3%; mean SPPB= 7.9 +/- 1.2; mean 25(OH)D=18.7 +/- 6.4 ng/ml). Adherence across supplement and placebo groups was similar (86% and 88%, respectively), and was also similar across groups for the physical activity intervention (75% and 72%, respectively). Both groups demonstrated an improvement in gait speed with no significant difference between those who received the nutritional supplement compared to the placebo (0.071 and 0.108 m/s, respectively (p=0.06)). Similar effects in physical function were observed using the SPPB. Serum 25(OH)D increased in supplemented group compared to placebo 7.4 ng/ml versus 1.3 ng/ml respectively. Results suggest improved gait speed following physical activity program with no further improvement with added nutritional supplementation.
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| 23. |
- Forsberg, U., et al.
(author)
-
A new assessment of the alleged link between element 115 and element 117 decay chains
- 2016
-
In: Physics Letters. Section B: Nuclear, Elementary Particle and High-Energy Physics. - : Elsevier BV. - 0370-2693. ; 760, s. 293-296
-
Journal article (peer-reviewed)abstract
- A novel rigorous statistical treatment is applied to available data (May 9, 2016) from search and spectroscopy experiments on the elements with atomic numbers Z = 115 and Z = 117 . The present analysis implies that the hitherto proposed cross-reaction link between α-decay chains associated with the isotopes 293-117 and 289-115 is highly improbable.
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| 24. |
- Forsberg, U., et al.
(author)
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Recoil-α-fission and recoil-α-α-fission events observed in the reaction 48Ca + 243Am
- 2016
-
In: Nuclear Physics, Section A. - : Elsevier BV. - 0375-9474. ; 953, s. 117-138
-
Journal article (peer-reviewed)abstract
- Products of the fusion-evaporation reaction 48Ca + 243Am were studied with the TASISpec set-up at the gas-filled separator TASCA at the GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany. Amongst the detected thirty correlated α-decay chains associated with the production of element Z=115, two recoil-α-fission and five recoil-α-α-fission events were observed. The latter five chains are similar to four such events reported from experiments performed at the Dubna gas-filled separator, and three such events reported from an experiment at the Berkeley gas-filled separator. The four chains observed at the Dubna gas-filled separator were assigned to start from the 2n-evaporation channel 289115 due to the fact that these recoil-α-α-fission events were observed only at low excitation energies. Contrary to this interpretation, we suggest that some of these recoil-α-α-fission decay chains, as well as some of the recoil-α-α-fission and recoil-α-fission decay chains reported from Berkeley and in this article, start from the 3n-evaporation channel 288115.
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| 25. |
- Forsberg, Ulrika, et al.
(author)
-
Spectroscopic Tools Applied to Element Z = 115 Decay Chains
- 2014
-
In: EPJ Web of Conferences. - : EDP Sciences. - 2100-014X .- 2101-6275. - 9782759811755 - 9782759811762 ; 66, s. 02036-02036
-
Conference paper (peer-reviewed)abstract
- Nuclides that are considered to be isotopes of element Z = 115 were produced in the reaction 48Ca + 243Am at the GSI Helmholtzzentrum für Schwerionenforschung Darmstadt. The detector setup TASISpec was used. It was mounted behind the gas-filled separator TASCA. Thirty correlated α-decay chains were found, and the energies of the particles were determined with high precision. Two important spectroscopic aspects of the offline data analysis are discussed in detail: the handling of digitized preamplified signals from the silicon strip detectors, and the energy reconstruction of particles escaping to upstream detectors relying on pixel-by-pixel dead-layer thicknesses.
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| 26. |
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| 27. |
- Hawkins, Stephen J., et al.
(author)
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The Intertidal Zone of the North-East Atlantic Region
- 2019
-
In: Interactions in the Marine Benthos: Global Patterns and Processes (Systematics Association Special Volume Series, pp. 7-46). - : Cambridge university press. - 9781108416085
-
Book chapter (peer-reviewed)abstract
- The rocky shores of the north-east Atlantic have been long studied. Our focus is from Gibraltar to Norway plus the Azores and Iceland. Phylogeographic processes shape biogeographic patterns of biodiversity. Long-term and broadscale studies have shown the responses of biota to past climate fluctuations and more recent anthropogenic climate change. Inter- and intra-specific species interactions along sharp local environmental gradients shape distributions and community structure and hence ecosystem functioning. Shifts in domination by fucoids in shelter to barnacles/mussels in exposure are mediated by grazing by patellid limpets. Further south fucoids become increasingly rare, with species disappearing or restricted to estuarine refuges, caused by greater desiccation and grazing pressure. Mesoscale processes influence bottom-up nutrient forcing and larval supply, hence affecting species abundance and distribution, and can be proximate factors setting range edges (e.g., the English Channel, the Iberian Peninsula). Impacts of invasive non-native species are reviewed. Knowledge gaps such as the work on rockpools and host–parasite dynamics are also outlined.
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| 28. |
- Hinwood, M., et al.
(author)
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Do P2Y12 receptor inhibitors prescribed poststroke modify the risk of cognitive disorder or dementia? Protocol for a target trial using multiple national Swedish registries
- 2022
-
In: Bmj Open. - : BMJ. - 2044-6055. ; 12:5
-
Journal article (peer-reviewed)abstract
- Introduction The target of a class of antiplatelet medicines, P2Y12R inhibitors, exists both on platelets and on brain immune cells (microglia). This protocol aims to describe a causal (based on a counterfactual model) approach for analysing whether P2Y12R inhibitors prescribed for secondary prevention poststroke may increase the risk of cognitive disorder or dementia via their actions on microglia, using real-world evidence. Methods and analysis This will be a cohort study nested within the Swedish National Health and Medical Registers, including all people with incident stroke from 2006 to 2016. We developed directed acyclic graphs to operationalise the causal research question considering potential time-independent and time-dependent confounding, using input from several experts. We developed a study protocol following the components of the target trial approach described by Hernan et al and describe the data structure that would be required in order to make a causal inference. We also describe the statistical approach required to derive the causal estimand associated with this important clinical question; that is, a time-to-event analysis for the development of cognitive disorder or dementia at 1, 2 and 5-year follow-up, based on approaches for competing events to account for the risk of all-cause mortality. Causal effect estimates and the precision in these estimates will be quantified. Ethics and dissemination This study has been approved by the Ethics Committee of the University of Gothenburg and Confidentiality Clearance at Statistics Sweden with Dnr 937-18, and an approved addendum with Dnr 2019-0157. The analysis and interpretation of the results will be heavily reliant on the structure, quality and potential for bias of the databases used. When we implement the protocol, we will consider and document any biases specific to the dataset and conduct appropriate sensitivity analyses. Findings will be disseminated to local stakeholders via conferences, and published in appropriate scientific journals.
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| 29. |
- Höglund Åberg, Carola, et al.
(author)
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Progression of attachment loss is strongly associated with presence of the JP2 genotype of Aggregatibacter actinomycetemcomitans: a prospective cohort study of a young adolescent population
- 2014
-
In: Journal of Clinical Periodontology. - : Wiley. - 0303-6979 .- 1600-051X. ; 41:3, s. 232-241
-
Journal article (peer-reviewed)abstract
- Aim: To assess the progression of attachment loss (AL) during a 2-year period according to the presence of JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans in a Ghanaian adolescent population. MethodsA total of 500 adolescents (mean age 13.2years, SD1.5) were enrolled in the study. After 2years, 397 (79.4%) returned for a periodontal re-examination, including the measurement of AL. The carrier status of the JP2 and non-JP2 genotypes of A. actinomycetemcomitans was evaluated in a baseline examination 2years earlier. ResultsIndividuals who carried the JP2 genotype of A. actinomycetemcomitans had a significantly increased risk [relative risk (RR)=7.3] of developing AL3mm. The mean AL at the follow-up and the mean 2-year progression of AL were significantly higher in the JP2 genotype-positive group (n=38) compared with the group positive for the non-JP2 genotypes of A. actinomycetemcomitans (n=169), and the group of A. actinomycetemcomitans-negative individuals (n=190). The JP2 genotype was strongly associated with the progression of AL3mm (OR=14.3). The non-JP2 genotypes of A. actinomycetemcomitans were also, however, less pronounced, associated with the progression of AL3mm (OR=3.4). Conclusion: The JP2 genotype of A. actinomycetemcomitans is strongly associated with the progression of AL.
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| 30. |
- Jenkins, S. R., et al.
(author)
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Spatial and temporal variation in settlement and recruitment of the intertidal barnacle Semibalanus balanoides (L.) (Crustacea : Cirripedia) over a European scale
- 2000
-
In: Journal of Experimental Marine Biology and Ecology. - 0022-0981. ; 243:2, s. 209-225
-
Journal article (peer-reviewed)abstract
- Variation in the level of settlement and recruitment in the intertidal barnacle Semibalanus balanoides was studied using a hierarchical sampling programme. The effect of three spatial scales, 10s of metres (sites), 1000s of metres (shores) and 100s of kilometres (locations), was determined. The largest spatial scale represented the distance between four widely separated locations, Sweden, the Isle of Man, SW Ireland and SW England, covering a. large part of the range of S. balanoides in Europe. Temporal variation was determined by comparison between two years, 1997 and 1998. The settlement period of S. balanoides varied in length and timing, being earlier and shorter at the most northerly location, Sweden. The duration of settlement showed little difference among shores within locations, but the pattern of settlement did vary. Estimates of total settlement throughout the settlement period and of recruitment at the end of this period both showed substantial variation among locations which was dependent on the year of study. There was little consistency in the ranking of locations between the two years. Recruitment showed significant variation I the lower spatial scales of shore and site. In addition, examination of variance components showed a high degree of variation between replicates within sites in 1997. There was a significant relationship between settlement and recruitment at three of the four locations. Across all locations variation in settlement explained between 29 and 99% of variation in recruitment. However, locations showed distinct differences in the level of post-settlement survival. (C) 2000 Elsevier Science B.V. All rights reserved.
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| 31. |
- Kirn, Dylan R., et al.
(author)
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The Vitality, Independence, and Vigor in the Elderly 2 Study (VIVE2) : Design and methods
- 2015
-
In: Contemporary Clinical Trials. - : Elsevier BV. - 1551-7144 .- 1559-2030. ; 43, s. 164-171
-
Journal article (peer-reviewed)abstract
- Background: Nutritional supplementation may potentiate the increase in skeletal muscle protein synthesis following exercise in healthy older individuals. Whether exercise and nutrition act synergistically to produce sustained changes in physical functioning and body composition has not been well studied, particularly in mobility-limited older adults. Methods: The VIVE2 study was a multi-center, randomized controlled trial, conducted in the United States and Sweden. This study was designed to compare the effects of a 6-month intervention with a once daily, experimental, 4 fl. oz. liquid nutritional supplement providing 150 kcal, whey protein (20 g), and vitamin D (800 IU) (Nestle Health Science, Vevey, Switzerland), to a low calorie placebo drink (30 kcal, non-nutritive; identical format) when combined with group-based exercise in 150 community-dwelling, mobility-limited older adults. All participants participated in a structured exercise program (3 sessions/week for 6 months), which included aerobic, strength, flexibility, and balance exercises. Results: The primary outcome was 6-month change in 400 m walk performance (m/s) between supplement and placebo groups. Secondary outcomes included 6 month change in: body composition, muscle cross-sectional area, leg strength, grip strength, stair climb time, quality of life, physical performance, mood/depressive symptoms and nutritional status. These outcomes were selected based on their applicability to the health and wellbeing of older adults. Conclusions: The results of this study will further define the role of nutritional supplementation on physical functioning and restoration of skeletal muscle mass in older adults. Additionally, these results will help refine the current physical activity and nutritional recommendations for mobility-limited older adults.
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| 32. |
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| 33. |
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| 34. |
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| 35. |
- Njobeh, P. B., et al.
(author)
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Estimation of multi-mycotoxin contamination in South African compound feeds
- 2012
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In: Toxins. - : MDPI AG. - 2072-6651. ; 4:10, s. 836-848
-
Journal article (peer-reviewed)abstract
- A total of 92 commercial compound feeds from South Africa were investigated for various mycotoxins. The data reveal the highest incidence of feed contamination for fumonisins (FB) (range: 104-2999 μg/kg) followed by deoxynivalenol (DON) (range: 124-2352 μg/kg) and zearalenone (ZEA) (range: 30-610 μg/kg). The incidence of ochratoxin A (OTA) and aflatoxins (AF)-contaminated samples were generally low, i.e., 4% and 30% of samples with levels ranging between 6.4 and 17.1 μg/kg (mean: 9.9 μg/kg) for OTA and 0.2 to 71.8 μg/kg (mean: 9.0 μg/kg) for AF. No samples contained T-2 toxin or HT-2 toxin. However, all samples analyzed were contaminated with at least one mycotoxin with a majority containing several mycotoxins. In particular, 3 of 4 positive samples mainly cattle feeds that had relatively high contents of OTA (ranging from 7 to 17.1 μg/kg) also contained high amounts of AF (>27.5 μg/kg) together with FB, DON and ZEA. Apart from a few samples, the levels of mycotoxins may be regarded as safe for livestock production in South Africa. However, the persistent co-occurrence of mycotoxins in samples, especially those at high concentrations, i.e., AF and OTA, together with other mycotoxins studied, may elicit synergistic or additive effects in animals. This should raise concern as multiple contaminations may pose a risk to livestock production and health.
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| 36. |
- Olsen, J. L., et al.
(author)
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The phylogeographic architecture of the fucoid seaweed Ascophyllum nodosum: an intertidal 'marine tree' and survivor of more than one glacial-interglacial cycle
- 2010
-
In: Journal of Biogeography. - : Wiley. - 0305-0270 .- 1365-2699. ; 37:5, s. 842-856
-
Journal article (peer-reviewed)abstract
- Aim Ascophyllum nodosum (L.) Le Jolis is a dominant fucoid seaweed occurring along sheltered, rocky shores throughout the North Atlantic (but not in the Pacific), where it is a foundational species of the intertidal community. Its large size and vulnerability to ice-scour have led to the hypothesis that contemporary populations in the north-west Atlantic may be the result of de novo recolonization from the north-east Atlantic since the Last Glacial Maximum (LGM) (c. 20 ka). We tested this hypothesis. Location Temperate North Atlantic rocky intertidal between c. 42 and 65 degrees N latitude. Methods More than 1300 individuals from 28 populations were sampled from across the entire range of A. nodosum and genotyped for six microsatellite loci, and > 500 individuals were genotyped for two mitochondrial loci, an intergenic spacer (IGS) and the tRNA (W) gene (trnW). Population structure and historical demography were analysed in a standard population genetics and coalescence framework. Results Based on the presence of private alleles and haplotypes, we found that A. nodosum has survived on both sides of the Atlantic (since before the LGM, dating back to at least the penultimate Eemian interglacial) with similar effective population sizes and divergence times (1.2 and 0.8 Ma). Dispersal has been predominantly from Europe to North America, and there is very weak present-day population differentiation across the North Atlantic. Diversity measures provided additional support for determining the location of refugia. Main conclusions Ascophyllum nodosum was apparently little affected by the LGM, although contemporary climate change is likely to have major effects on its latitudinal distribution on both sides of the North Atlantic. It is a very long-lived species, analogous in virtually all demographic aspects to a tree - resistant to extinction but vulnerable to catastrophic events. The Brittany peninsula is a hotspot of genetic diversity worthy of conservation.
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| 37. |
- Ong, L. K., et al.
(author)
-
Growth Hormone Improves Cognitive Function After Experimental Stroke
- 2018
-
In: Stroke. - : Ovid Technologies (Wolters Kluwer Health). - 0039-2499 .- 1524-4628. ; 49:5, s. 1257-1266
-
Journal article (peer-reviewed)abstract
- Background and Purpose-Cognitive impairment is a common outcome for stroke survivors. Growth hormone (GH) could represent a potential therapeutic option as this peptide hormone has been shown to improve cognition in various clinical conditions. In this study, we evaluated the effects of peripheral administration of GH at 48 hours poststroke for 28 days on cognitive function and the underlying mechanisms. Methods-Experimental stroke was induced by photothrombotic occlusion in young adult mice. We assessed the associative memory cognitive domain using mouse touchscreen platform for paired-associate learning task. We also evaluated neural tissue loss, neurotrophic factors, and markers of neuroplasticity and cerebrovascular remodeling using biochemical and histology analyses. Results-Our results show that GH-treated stroked mice made a significant improvement on the paired-associate learning task relative to non-GH-treated mice at the end of the study. Furthermore, we observed reduction of neural tissue loss in GH-treated stroked mice. We identified that GH treatment resulted in significantly higher levels of neurotrophic factors (IGF-1 [insulin-like growth factor-1] and VEGF [vascular endothelial growth factor]) in both the circulatory and peri-infarct regions. GH treatment in stroked mice not only promoted protein levels and density of presynaptic marker (SYN-1 [synapsin-1]) and marker of myelination (MBP [myelin basic protein]) but also increased the density and area coverage of 2 major vasculature markers (CD31 and collagen-IV), within the peri-infarct region. Conclusions-These findings provide compelling preclinical evidence for the usage of GH as a potential therapeutic tool in the recovery phase of patients after stroke. Visual Overview-An online visual overview is available for this article.
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| 38. |
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| 39. |
- Rudolph, Dirk, et al.
(author)
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Alpha-Photon Coincidence Spectroscopy Along Element 115 Decay Chains
- 2014
-
In: Acta Physica Polonica. Series B: Elementary Particle Physics, Nuclear Physics, Statistical Physics, Theory of Relativity, Field Theory. - 0587-4254. ; 45, s. 263-272
-
Journal article (peer-reviewed)abstract
- Produced in the reaction 48Ca+243Am, thirty correlated α-decay chains were observed in an experiment conducted at the GSI Helmholzzentrum für Schwerionenforschung, Darmstadt, Germany. The decay chains are basically consistent with previous findings and are considered to originate from isotopes of element 115 with mass numbers 287, 288, and 289. A set-up aiming specifically for high-resolution charged particle and photon coincidence spectroscopy was placed behind the gas-filled separator TASCA. For the first time, γ rays as well as X-ray candidates were observed in prompt coincidence with the α-decay chains of element 115.
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| 40. |
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| 41. |
- Rudolph, Dirk, et al.
(author)
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Selected Spectroscopic Results on Element 115 Decay Chains
- 2015
-
In: Journal of Radioanalytical and Nuclear Chemistry. - : Springer Science and Business Media LLC. - 0236-5731 .- 1588-2780. ; 303:2, s. 1185-1190
-
Journal article (peer-reviewed)abstract
- Thirty correlated alpha-decay chains were observed in an experiment studying the fusion-evaporation reaction 48Ca + 243Am at the GSI Helmholtzzentrum f¨ur Schwerionenforschung. The decay characteristics of the majority of these 30 chains are consistent with previous observations and interpretations of such chains to originate from isotopes of element Z = 115. High-resolution alpha-photon coincidence spectroscopy in conjunction with comprehensive Monte-Carlo simulations allow to propose excitation schemes of atomic nuclei of the heaviest elements, thereby probing nuclear structure models near the ‘Island of Stability’ with unprecedented experimental precision.
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| 42. |
- Rudolph, Dirk, et al.
(author)
-
Spectroscopy of Element 115 Decay Chains
- 2013
-
In: Physical Review Letters. - 1079-7114. ; 111:11
-
Journal article (peer-reviewed)abstract
- A high-resolution , X-ray and gamma-ray coincidence spectroscopy experiment was conducted at the GSI Helmholtzzentrum für Schwerionenforschung. Thirty correlated alpha-decay chains were detected following the fusion-evaporation reaction 48Ca + 243Am. The observations are consistent with previous assignments of similar decay chains to originate from element Z = 115. For the first time, precise spectroscopy allows the derivation of excitation schemes of isotopes along the decay chains starting with elements Z > 112. Comprehensive Monte-Carlo simulations accompany the data analysis. Nuclear structure models provide a first level interpretation.
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| 43. |
- Sanchez-Bezanilla, S., et al.
(author)
-
Growth Hormone Increases BDNF and mTOR Expression in Specific Brain Regions after Photothrombotic Stroke in Mice
- 2022
-
In: Neural Plasticity. - : Hindawi Limited. - 2090-5904 .- 1687-5443. ; 2022
-
Journal article (peer-reviewed)abstract
- Aims. We have shown that growth hormone (GH) treatment poststroke increases neuroplasticity in peri-infarct areas and the hippocampus, improving motor and cognitive outcomes. We aimed to explore the mechanisms of GH treatment by investigating how GH modulates pathways known to induce neuroplasticity, focusing on association between brain-derived neurotrophic factor (BDNF) and mammalian target of rapamycin (mTOR) in the peri-infarct area, hippocampus, and thalamus. Methods. Recombinant human growth hormone (r-hGH) or saline was delivered (0.25 mu l/hr, 0.04 mg/day) to mice for 28 days, commencing 48 hours after photothrombotic stroke. Protein levels of pro-BDNF, total-mTOR, phosphorylated-mTOR, total-p70S6K, and phosporylated-p70S6K within the peri-infarct area, hippocampus, and thalamus were evaluated by western blotting at 30 days poststroke. Results. r-hGH treatment significantly increased pro-BDNF in peri-infarct area, hippocampus, and thalamus (p < 0.01). r-hGH treatment significantly increased expression levels of total-mTOR in the peri-infarct area and thalamus (p < 0.05). r-hGH treatment significantly increased expression of total-p70S6K in the hippocampus (p < 0.05). Conclusion. r-hGH increases pro-BDNF within the peri-infarct area and regions that are known to experience secondary neurodegeneration after stroke. Upregulation of total-mTOR protein expression in the peri-infarct and thalamus suggests that this might be a pathway that is involved in the neurorestorative effects previously reported in these animals and warrants further investigation. These findings suggest region-specific mechanisms of action of GH treatment and provide further understanding for how GH treatment promotes neurorestorative effects after stroke.
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| 44. |
- Sanchez-Bezanilla, S., et al.
(author)
-
Growth Hormone Promotes Motor Function after Experimental Stroke and Enhances Recovery-Promoting Mechanisms within the Peri-Infarct Area
- 2020
-
In: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 21:2
-
Journal article (peer-reviewed)abstract
- Motor impairment is the most common and widely recognised clinical outcome after stroke. Current clinical practice in stroke rehabilitation focuses mainly on physical therapy, with no pharmacological intervention approved to facilitate functional recovery. Several studies have documented positive effects of growth hormone (GH) on cognitive function after stroke, but surprisingly, the effects on motor function remain unclear. In this study, photothrombotic occlusion targeting the motor and sensory cortex was induced in adult male mice. Two days post-stroke, mice were administered with recombinant human GH or saline, continuing for 28 days, followed by evaluation of motor function. Three days after initiation of the treatment, bromodeoxyuridine was administered for subsequent assessment of cell proliferation. Known neurorestorative processes within the peri-infarct area were evaluated by histological and biochemical analyses at 30 days post-stroke. This study demonstrated that GH treatment improves motor function after stroke by 50%-60%, as assessed using the cylinder and grid walk tests. Furthermore, the observed functional improvements occurred in parallel with a reduction in brain tissue loss, as well as increased cell proliferation, neurogenesis, increased synaptic plasticity and angiogenesis within the peri-infarct area. These findings provide new evidence about the potential therapeutic effects of GH in stroke recovery.
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| 45. |
- Sanchez-Bezanilla, S., et al.
(author)
-
Growth Hormone Treatment Promotes Remote Hippocampal Plasticity after Experimental Cortical Stroke
- 2020
-
In: International Journal of Molecular Sciences. - : MDPI AG. - 1422-0067. ; 21:12
-
Journal article (peer-reviewed)abstract
- Cognitive impairment is common after stroke, and disturbances in hippocampal function are often involved, even in remote non-hippocampal injuries. In terms of hippocampal function, growth hormone (GH) is known to affects plasticity and cognition. We aimed to investigate whether GH treatment after an experimental cortical stroke could enhance remote hippocampal plasticity and the hippocampal-dependent visual discrimination task. C57BL6 male mice were subjected to cortical photothrombotic stroke. Stroke mice were then treated with either saline or GH at 48 h after occlusion for 28 days. We assessed learning and memory using mouse touchscreen platform for the visual discrimination task. We also evaluated markers of neural progenitor cells, synaptic plasticity and cerebrovascular remodelling in the hippocampal formation. GH treatment significantly improved the performance on visual discrimination task after stroke. We observed a concomitant increased number of bromodeoxyuridine-positive cells in the dentate gyrus of the hippocampus. We also detected increased protein levels and density of doublecortin, a neuronal precursor cells marker, as well as glutamate receptor 1 (GLuR1), a synaptic marker. These findings provide further neurobiological evidence for how GH treatment could be used to promote hippocampal plasticity in a remote region from the initial cortical injury, and thus enhance cognitive recovery after stroke.
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| 46. |
- Skarin, Hanna, et al.
(author)
-
The workshop on animal botulism in europe
- 2013
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In: Biosecurity and bioterrorism. - : Mary Ann Liebert Inc. - 1538-7135 .- 1557-850X. ; 11:SUPPL. 1, s. S183-S190
-
Journal article (peer-reviewed)abstract
- A workshop on animal botulism was held in Uppsala, Sweden, in June 2012. Its purpose was to explore the current status of the disease in Europe by gathering the European experts in animal botulism and to raise awareness of the disease among veterinarians and others involved in biopreparedness. Animal botulism is underreported and underdiagnosed, but an increasing number of reports, as well as the information gathered from this workshop, show that it is an emerging problem in Europe. The workshop was divided into 4 sessions: animal botulism in Europe, the bacteria behind the disease, detection and diagnostics, and European collaboration and surveillance. An electronic survey was conducted before the workshop to identify the 3 most needed discussion points, which were: prevention, preparedness and outbreak response; detection and diagnostics; and European collaboration and surveillance. The main conclusions drawn from these discussions were that there is an urgent need to replace the mouse bioassay for botulinum toxin detection with an in vitro test and that there is a need for a European network to function as a reference laboratory, which could also organize a European supply of botulinum antitoxin and vaccines. The foundation of such a network was discussed, and the proposals are presented here along with the outcome of discussions and a summary of the workshop itself. © 2013, Mary Ann Liebert, Inc.
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| 47. |
- Sousa, Pedro F. M., et al.
(author)
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Can we beat overfitting?-A closer look at Cloarec's PLS algorithm
- 2018
-
In: Journal of Chemometrics. - : Wiley. - 0886-9383 .- 1099-128X. ; 32:6
-
Journal article (peer-reviewed)abstract
- Random noise has been addressed as a cause of overfitting in partial least squares regression. A previous study pinpointed that one of the sources of overfitting resides in the calculation of scores due to the accumulation of noise in the diagonal of the variance-covariance matrix, and a modified partial least squares regression was proposed with the removal of this diagonal prior to the score calculation. Here, a further modification of the NIPALS algorithm is proposed, with the same ability to overcome overfitting due to noise, but algebraically more similar to the original NIPALS. The results indicate that it is possible to get more reliable auto-prediction R-2 with a cross-validation performance close to that of the original NIPALS algorithm.
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| 48. |
- Sousa, Pedro F. M., et al.
(author)
-
Elucidation of chromatographic peak shifts in complex samples using a chemometrical approach
- 2018
-
In: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 410:21, s. 5229-5235
-
Journal article (peer-reviewed)abstract
- Chromatographic retention time peak shifts between consecutive analyses is a well-known fact yet not fully understood. Algorithms have been developed to align peaks between runs, but with no specific studies considering the causes of peak shifts. Here, designed experiments reveal chromatographic shift patterns for a complex peptide mixture that are attributable to the temperature and pH of the mobile phase. These results demonstrate that peak shifts are highly structured and are to a high degree explained by underlying differences in physico-chemical parameters of the chromatographic system and also provide experimental support for the alignment algorithm called the generalized fuzzy Hough transform which exploits this fact. It can be expected that the development of alignment algorithms enters a new phase resulting in increasingly accurate alignment by considering the latent structure of the peak shifts.
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| 49. |
- Sousa, Pedro F.M. 1977-, et al.
(author)
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nLossFinder—A Graphical User Interface Program for the Nontargeted Detection of DNA Adducts
- 2021
-
In: Toxics. - : MDPI AG. - 2305-6304. ; 9:4
-
Journal article (peer-reviewed)abstract
- DNA adductomics is a relatively new omics approach aiming to measure known and unknown DNA modifications, called DNA adducts. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) has become the most common method for analyzing DNA adducts. Recent advances in the field of mass spectrometry have allowed the possibility to perform a comprehensive analysis of adducts, for instance, by using a nontargeted data-independent acquisition method, with multiple precursor m/z windows as an inclusion list. However, the generated data are large and complex, and there is a need to develop algorithms to simplify and automate the time-consuming manual analysis that has hitherto been used. Here, a graphical user interface (GUI) program was developed, with the purpose of tracking a characteristic neutral loss reaction from tandem mass spectrometry of the nucleoside adducts. This program, called nLossFinder, was developed in the MATLAB platform, available as open-source code. Calf thymus DNA was used as a model for method optimization, and the overall adductomics approach was applied to DNA from amphipods (Monoporeia affinis) collected within the Swedish National Marine Monitoring Program. In the amphipod DNA, over 150 putative adducts were found in comparison to 18 using a manual approach in a previous study. The developed program can improve the processing time for large MS data, as it processes each sample in a few seconds, and hence can be applicable for high-throughput screening of adducts.
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| 50. |
- Szatkiewicz, Jin, et al.
(author)
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The genomics of major psychiatric disorders in a large pedigree from Northern Sweden
- 2019
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In: Translational Psychiatry. - : Nature Publishing Group. - 2158-3188. ; 9
-
Journal article (peer-reviewed)abstract
- We searched for genetic causes of major psychiatric disorders (bipolar disorder, schizoaffective disorder, and schizophrenia) in a large, densely affected pedigree from Northern Sweden that originated with three pairs of founders born around 1650. We applied a systematic genomic approach to the pedigree via karyotyping (N = 9), genome-wide SNP arrays (N = 418), whole-exome sequencing (N = 26), and whole-genome sequencing (N = 10). Comprehensive analysis did not identify plausible variants of strong effect. Rather, pedigree cases had significantly higher genetic risk scores compared to pedigree and community controls.
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