| 1. |
- Zhang, Renyun, et al.
(author)
-
High-speed deposition of multilayer nanofilms using soap-film coating
- 2013
-
Conference paper (peer-reviewed)abstract
- High-speed deposition of multilayer nanofilms using soap-film coatingRenyun Zhang, Henrik A. Andersson, Mattias Andersson, Britta Andres, Per Edström, Sverker Edvardsson, Sven Forsberg, Magnus Hummelgård, Niklas Johansson, Kristoffer Karlsson, Hans-Erik Nilsson, Martin Olsen, Tetsu Uesaka, Thomas Öhlund & Håkan OlinDepartment of Applied Science and Design, Mid Sweden University, SE-85170 Sundsvall, SwedenEmail: renyun.zhang@miun.se or hakan.olin@miun.seCoating1 of thin films is of importance for making functionalized surfaces with applications in many fields from electronics to consumer packaging. To decrease the cost, large scale roll-to-roll2 coating techniques are usually done at high speed, for example, ordinary printing paper is coated at a speed of tens of meters per second by depositing micrometer thick layers of clay. However, nanometer thin films are harder to coat at high speed by wet-chemical methods, requiring special roll-to-roll vacuum techniques3 with the cost of higher complexity.Here, we report a simple wet chemical method for high-speed coating of films down to molecular thicknesses, called soap-film coating (SFC)4. The technique is based on forcing a substrate through a soap film that contains nanomaterials. In the simplest laboratory version, the films can be deposited by a hand-coating procedure set up in a couple of minutes. The method is quite general molecules or nanomaterials or sub-micrometer materials (Figure 1) with thicknesses ranging from less than a monolayer to several layers at speeds up to meters per second. The applications of soap-film coating is quite wide an we will show solar cells, electrochromic devices, optical nanoparticle crystals, and nano-film devices. We believe that the soap-film coating method is potentially important for industrial-scale nanotechnology.Fig. 1. Soap film coating of nanoparticles, layered materials, nanowires, and molecules. a sub-monolayer 240 nm silica nanoparticle (scale bar 2 µm) b monolayer c double layer. d monolayer gold nanoparticles. e single layer TiO2 nanoparticles. f sub-monolayer polystyrene (scale 2 µm), g monolayer of polystyrene. h triple-layer of polystyrene. i monolayer of Ferritin. j AFM image of <1.5 layer GO film (3 µm x 2 µm). k clay on glass (scale 2 µm). l SFC coated nanocellulose. m Absorbance spectra Rhodamine B on a glass slide. AFM of SDS layers n (2 µm x 1.5 µm) and o (20 µm x 15 µm).ReferencesTracton, A. A. Coating Technology Handbook (CRC Press, Boca Raton, 2006).Ohring, M. Materials science of thin films. (Academic press., 2001).Charles, B. Vacuum deposition onto webs, films and foils. (William Andrew, 2011).Zhang, R. Y., Andersson, H. A., Andersson, M., Andres, B., Edström, P., Edvardsson, S., Forsberg, S., Hummelgård, M., Johansson, N., Karlsson, K., Nilsson, H.-E., Olsen, M., Uesaka, T., Öhlund, T., Olin H. Soap film coating: High-speed deposition of multilayer nanofilms. Submitted.
|
|
| 2. |
- Zhang, Renyun, et al.
(author)
-
Soap-film coating : High-speed deposition of multilayer nanofilms
- 2013
-
In: Scientific Reports. - Nature Publishing Group : Springer Science and Business Media LLC. - 2045-2322. ; 3, s. Art. no. 1477-
-
Journal article (peer-reviewed)abstract
- The coating of thin films is applied in numerous fields and many methods are employed for the deposition of these films. Some coating techniques may deposit films at high speed; for example, ordinary printing paper is coated with micrometre-thick layers of clay at a speed of tens of meters per second. However, to coat nanometre thin films at high speed, vacuum techniques are typically required, which increases the complexity of the process. Here, we report a simple wet chemical method for the high-speed coating of films with thicknesses at the nanometre level. This soap-film coating technique is based on forcing a substrate through a soap film that contains nanomaterials. Molecules and nanomaterials can be deposited at a thickness ranging from less than a monolayer to several layers at speeds up to meters per second. We believe that the soap-film coating method is potentially important for industrial-scale nanotechnology.
|
|
| 3. |
- Öhlund, Thomas, et al.
(author)
-
Assisted sintering of silver nanoparticle inkjet inks on paper with active coatings
- 2015
-
In: RSC Advances. - : Royal Society of Chemistry (RSC). - 2046-2069. ; 5, s. 64841-64849
-
Journal article (peer-reviewed)abstract
- Inkjet-printed metal films are important within the emerging field of printed electronics. For large-scale manufacturing, low-cost flexible substrates and low temperature sintering is desired. Tailored coated substrates are interesting for roll-to-roll fabrication of printed electronics, since a suitable tailoring of the ink-substrate system may reduce, or remove, the need for explicit sintering. Here we utilize specially designed coated papers, containing chloride as an active sintering agent. The built-in sintering agent greatly assists low-temperature sintering of inkjet-printed AgNP films. Further, we examine the effect of variations in coating pore size and precoating type. Interestingly, we find that the sintering is substantially affected by these parameters.
|
|
| 4. |
- Alecrim, Viviane, et al.
(author)
-
Exfoliated Layered Materials for Digital Fabrication
- 2015
-
In: NIP & Digital Fabrication Conference. ; , s. 192-194
-
Conference paper (peer-reviewed)abstract
- We introduced an exfoliation method of MoS2 in a 3% solution of sodium dodecyl surfactant at high concentration (i.e. 2 g/L). The bulk MoS2 was thinned by mechanical exfoliation between sand papers and the resulting powder was used to prepare dispersions by liquid exfoliation through probe sonication. The resulting dispersion consisted of very thin MoS2 nanosheets in surfactant solution with average lateral size around 126 nm. This may be interesting for applications in inkjet printed electronics.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
- Andersson, Björn, 1977, et al.
(author)
-
Development of a machine learning framework for radiation biomarker discovery and absorbed dose prediction.
- 2023
-
In: Frontiers in oncology. - 2234-943X. ; 13
-
Journal article (peer-reviewed)abstract
- Molecular radiation biomarkers are an emerging tool in radiation research with applications for cancer radiotherapy, radiation risk assessment, and even human space travel. However, biomarker screening in genome-wide expression datasets using conventional tools is time-consuming and underlies analyst (human) bias. Machine Learning (ML) methods can improve the sensitivity and specificity of biomarker identification, increase analytical speed, and avoid multicollinearity and human bias.To develop a resource-efficient ML framework for radiation biomarker discovery using gene expression data from irradiated normal tissues. Further, to identify biomarker panels predicting radiation dose with tissue specificity.A strategic search in the Gene Expression Omnibus database identified a transcriptomic dataset (GSE44762) for normal tissues radiation responses (murine kidney cortex and medulla) suited for biomarker discovery using an ML approach. The dataset was pre-processed in R and separated into train and test data subsets. High computational cost of Genetic Algorithm/k-Nearest Neighbor (GA/KNN) mandated optimization and 13 ML models were tested using the caret package in R. Biomarker performance was evaluated and visualized via Principal Component Analysis (PCA) and dose regression. The novelty of ML-identified biomarker panels was evaluated by literature search.Caret-based feature selection and ML methods vastly improved processing time over the GA approach. The KNN method yielded overall best performance values on train and test data and was implemented into the framework. The top-ranking genes were Cdkn1a, Gria3, Mdm2 and Plk2 in cortex, and Brf2, Ccng1, Cdkn1a, Ddit4l, and Gria3 in medulla. These candidates successfully categorized dose groups and tissues in PCA. Regression analysis showed that correlation between predicted and true dose was high with R2 of 0.97 and 0.99 for cortex and medulla, respectively.The caret framework is a powerful tool for radiation biomarker discovery optimizing performance with resource-efficiency for broad implementation in the field. The KNN-based approach identified Brf2, Ddit4l, and Gria3 mRNA as novel candidates that have been uncharacterized as radiation biomarkers to date. The biomarker panel showed good performance in dose and tissue separation and dose regression. Further training with larger cohorts is warranted to improve accuracy, especially for lower doses.
|
|
| 10. |
|
|
| 11. |
|
|
| 12. |
|
|
| 13. |
- Andersson, Britta, 1979-
(author)
-
Manipulation of potassium ion fluxes to induce apoptosis in lung cancer cells
- 2007
-
Doctoral thesis (other academic/artistic)abstract
- Apoptosis is a special form of cell death that if non-functional may lead to diseases such as cancer. A reduction of the intracellular potassium ion (K+) content is necessary for activating enzymes important for the execution of apoptosis. Pharmacological modulation of K+ fluxes to reduce intracellular K+ in cancer cells might therefore force the cells into apoptosis and decrease tumour cell mass. Human malignant pleural mesothelioma (MPM) is a form of cancer often caused by asbestos exposure. Although asbestos has been banned in the Western World, the incidence of MPM is expected to increase. Cisplatin is the first-line chemotherapy for MPM, but acquired resistance to the drug is a clinical problem. This thesis is mainly based on work with the human malignant pleural mesothelioma cell line (P31 wt) and a cisplatin-resistant sub-line (P31 res). The aim was to first characterize K+ fluxes in P31 wt and P31 res cells, and then manipulate them in order to reduce intracellular K+ and induce apoptosis with K+ manipulation alone or in combination with cisplatin. Characterization of K+ fluxes in P31 wt cells showed that: 1) ouabain, a digitalis-like drug, and specific blocker of the Na+, K+, ATPase pump, effectively inhibited K+ uptake, 2) bumetanide, a diuretic, and an inhibitor of the Na+, K+, 2Cl-¬-cotransporter, had a transient effect on K+ uptake, and 3) the antifungal drug amphotericin B stimulated K+ efflux. In order to determine intracellular K+ content, the potassium-binding fluorescent probe PBFI-AM was used in a 96-well plate assay. After a 3-h incubation with ouabain, with or without bumetanide, combined with amphotericin B, the intracellular K+ content was reduced in P31 wt cells but not in P31 res cells. Ouabain induced apoptosis in both P31 wt and P31 res cells. P31 res cells were sensitized to cisplatin by ouabain, since 10 mg/L cisplatin in combination with ouabain induced about the same percentage of apoptotic cells as 40 mg/L cisplatin. Apoptosis was executed via caspase-3 activation in both P31 wt and P31 res cells. Amphotericin B enhanced ouabain-induced apoptosis in P31 wt cells via caspase-9 activation, with increased caspase-3 activation and DNA fragmentation as consequences. Ouabain-induced apoptosis in P31 res cells was executed via increased expression of pro-apoptotic Bak. The combination of cisplatin with ouabain and amphotericin B was stressful to both P31 wt and P31 res cells, since SAPK/JNK a known factor in stress-induced apoptosis was activated. In conclusion, K+ flux manipulation with clinical used drugs can induce apoptosis per se and also enhance cisplatin-induced apoptosis in P31 wt and P31 res cells.
|
|
| 14. |
|
|
| 15. |
|
|
| 16. |
|
|
| 17. |
- Andersson, Charlotte, et al.
(author)
-
Biodistribution of I-131 in mice is influenced by circadian variations
- 2020
-
In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
-
Journal article (peer-reviewed)abstract
- Effects of radiation and biodistribution of radionuclides are often studied in animal models. Circadian rhythm affects many biological functions and may influence the biokinetics of radionuclides and observed responses. The aim of this study was to investigate if the time during the day of I-131 injection affects the biodistribution and absorbed dose to tissues in mice. Biodistribution studies were conducted on male C57BL/6 N mice for three diurnal time-series: the animals were i.v. injected with 160 kBq I-131 at 8 am, 12 pm or 4 pm. The activity concentration in organs and tissues was measured at 1 h to 7 days after administration and absorbed dose at day 7 was determined. Comparison between the three time-series showed statistically significant differences in activity concentration in all investigated tissues and organs. Administration performed at 12 pm resulted in general in higher absorbed dose to the organs than injection performed at 8 am and 4 pm. Time of day of administration affects the biodistribution of I-131 in mice and consequently the absorbed dose to individual organs. These findings advocate that subsequent biodistribution studies and dosimetry calculations should consider time-point of administration as a variable that could influence the results.
|
|
| 18. |
|
|
| 19. |
|
|
| 20. |
- Andersson, Charlotte, et al.
(author)
-
Diurnal variations in biodistribution of the radionuclide I-131 in mice
- 2016
-
In: Swedish Cancer Research Meeting, Gothenburg, 2016, November 7-8.
-
Conference paper (other academic/artistic)abstract
- Background: Radionuclides are routinely used to diagnose and treat many different types of cancer. I-131 is a well-established radioisotope used in e.g. treatment of thyroid cancer and neuroblastoma. Accurate knowledge of I-131 biodistribution is essential to correctly estimate the absorbed dose to normal organs and determine potential risks from I-131 exposure, which is especially important when treating children. Many biological functions in living organisms follow a circadian rhythm. Nevertheless, little is known about diurnal variations in radionuclide biodistribution. This study investigates if circadian rhythm affects I-131 biodistribution in mice and absorbed dose to organs and tissues. Materials & Methods: The radioactivity concentration in mice tissues was studied at different time-points after administration of I-131, and absorbed doses were calculated. The effect of circadian rhythm was studied by varying the time of administration. Results: Difference in activity concentration between the administration time-points was observed at many time-points after administration for most investigated tissues. For some organs differences were also observed in the absorbed dose. The highest activity concentration and absorbed dose were found in the thyroid regardless of time of administration. Conclusion: The results demonstrate that the biodistribution of I-131 in mice is influenced by the time of day of administration. These findings advocate that circadian rhythm should be considered in biodistribution studies and suggests that time-point of administration of radiopharmaceuticals containing I-131 for therapy can be further optimized. An optimized time-point could result in higher absorbed dose to the tumor and/or lower absorbed dose to normal tissues.
|
|
| 21. |
- Andersson, Christian, et al.
(author)
-
Mobila strategier. En rapport från arbetsgruppen Nya medier
- 2007
-
Reports (other academic/artistic)abstract
- Utredningens fokus skall ligga på Bibliotek och ITs framtida hantering av mobila tjänster, läsplattor, ljud och bildfiler i olika format och dess tillgängliggöranden, MP3-spelare, dator- och TV-spel. Texten kan ses som en omvärldsanalys av möjligheter och scenarion kring arbetet med ovan nämnda tjänster och produkter. Förhoppningen är att texten ska peka på möjliga fokus för organisationen, stimulera till idéproduktion och vara avstampen för nya arbetstrupper för Bibliotek och IT.
|
|
| 22. |
- Andersson, Charlotte, et al.
(author)
-
The influence of circadian rhythm on the biodistribution of I-131
- 2016
-
In: Swedish Radiation Research Association for Young Scientists Workshop, Stockholm, 2016, August 25-26.
-
Conference paper (other academic/artistic)abstract
- Background: I-131 is well-established in nuclear medicine. The thyroid is a target organ when treating thyroid cancer with unbound I-131, but also a risk organ in I-131-based radionuclide therapy. Exposure to I-131 can also occur from the environment at nuclear accidents. Accurate knowledge of I-131 biodistribution is essential to correctly estimate the absorbed dose to organs and determine potential risks from both medical and hazard exposure. Many biological functions follow a circadian rhythm. Nevertheless, circadian rhythm remains an unknown factor in radionuclide biodistribution. Aim: The purpose of this study was to investigate if circadian rhythm affects I-131 biodistribution in mice and hence absorbed dose to mouse tissues. Methods: The radioactivity concentration in various tissues was studied at different time points after administration of I-131 and absorbed doses were calculated according to the MIRD formalism. The effect of circadian rhythm was studied by varying the time of administration. Male C57BL/6N mice were i.v. injected with I-131 at 8 am, 12 pm or 4 pm and killed after 1h to 7d. Results: Statistically significant difference in activity concentration and absorbed dose between the three injection series was observed for at least one time point after injection for many tissues. Highest activity concentration and absorbed dose were found in the thyroid. Conclusion: The results demonstrated that the biodistribution of I-131 in mice is influenced by the time of day of administration to a certain extent. These findings advocate that circadian rhythm should be considered in biodistribution studies and dose calculations.
|
|
| 23. |
- Andersson, Fia, 1950-
(author)
-
Att utmana erfarenheter : Kunskapsutveckling i en forskningscirkel
- 2007
-
Doctoral thesis (other academic/artistic)abstract
- This thesis aims at describing and analysing the process and content in knowledge development within a research circle. The participants in this circle are seven teachers who work with multilingual children diagnosed within the autism spectrum, and me as a researcher.The study is conducted within the tradition of participatory-oriented research. The research issue concerns the questions these teachers ask themselves in their everyday work. The study, consisting of twelve meetings, was carried out during 2004-2005. In the final meeting material collected in the circle was analysed together by all participants. The knowledge-content analysis disclosed four main themes: mother-tongue issues, disabilities and diagnoses, the issue of frames and matters related to working with parents and other professionals. The results show that once a child is diagnosed within the autism spectrum the diagnosis “takes over” and mother-tongue instruction is seldom discussed.The participants in the circle found it difficult to collaborate with the various authorities involved in working with a child and its family. They also found it difficult to communicate with parents, due to language barriers, different cultural contexts, and the observation that interpreters did not translate properly. The participants noted an existing hierarchy in relation to doctors and psychologists, regarded as having the mandate to assess a child’s ability and suggest placement in class.A conclusion is that the work of the teachers entails a high degree of complexity, and that knowledge meetings and collaboration between parents, teachers, and the various authorities are needed. During the circle process emancipating collective knowledge was constructed transcending what any participant had from the start. Experiences discussed in continuing dialogues, and in an on-going process, seem to be essential for generation of knowledge. When experiences were challenged, potentials for different actions were revealed.
|
|
| 24. |
|
|
| 25. |
- Andersson, Henrik, et al.
(author)
-
Contacting paper-based supercapacitors to printed electronics on paper substrates
- 2012
-
In: Nordic Pulp & Paper Research Journal. - 0283-2631 .- 2000-0669. ; 27:2, s. 476-480
-
Journal article (peer-reviewed)abstract
- Hybrid printed electronics, in which printed structures and silicon-based components co-exist will likely be among the first commercial solutions. In this case the paper substrate acts much in the same way as circuit boards, containing conductive tracks and acting as a carrier for the electrical components. It is important to consider the contacting of the components to be able to produce low resistance electrical contacts to the conductive tracks. Supercapacitors are able to deliver a large amount of current in a short time and are a good option for short term energy storage and if the printed product is to be used only one, or a few times, it can be the only power source needed. When manufacturing printed electronics, the overall resistance of the printed tracks as well as the contact resistance of the mounted components will add up to the total resistance of the system. A high resistance will cause a voltage drop from the power source to the component. This will waste power that goes to Joule heating and also the voltage and current available to components may be too low to drive them. If the intention is to use a power supply such as batteries or solar cells this becomes a limitation. In this article have been tested several conductive adhesives used to contact paper based supercapacitors to ink jet printed silver tracks on paper. The best adhesive gives about 0.3 Ω per contact, a factor 17 better compared to the worst which gave 5 Ω. The peak power that is possible to take out from a printed system with a flexible battery and super capacitors is about 10 times higher than compared with the same system with only the battery.
|
|
| 26. |
|
|
| 27. |
|
|
| 28. |
- Andres, Britta, et al.
(author)
-
Supercapacitors with graphene coated paper electrodes
- 2012
-
In: Nordic Pulp & Paper Research Journal. - 0283-2631 .- 2000-0669. ; 27:2, s. 481-485
-
Journal article (peer-reviewed)abstract
- Paper based supercapacitors are prepared by stacking a paper between two graphene electrodes and soaking these in an aqueous electrolyte. We demonstrate that supercapacitors can easily be manufactured by using proven paper technologies. Several different electrode materials were compared and two types of contacting material, silver and graphite foil were tested. The influence of the paper used as separator was also investigated. The supercapacitors with a graphene-gold nanoparticle composite as electrodes showed a specific capacitance of up to 100 F/g and an energy density of 1.27 Wh/kg. The energy density can further be increased by using other electrolytes. The silver contacts showed a pseudo capacitance, which the graphite contacts did not. The papers tested had a minor effect on the capacitance, but they have an influence on the weight and the volume of the supercapacitor.
|
|
| 29. |
|
|
| 30. |
|
|
| 31. |
|
|
| 32. |
|
|
| 33. |
- Elvborn, Mikael, et al.
(author)
-
The influence of biological sex on thyroid cancer treatment risk assessment
- 2016
-
In: Swedish Cancer Research Meeting, Gothenburg, 2016, November 7-8.
-
Conference paper (other academic/artistic)abstract
- Background: Researchers are often reluctant towards using females in studies, especially when radiopharmaceuticals and hormonally dependent diseases are concerned. Simultaneously, women are more prone to thyroid-related diseases such as Grave’s disease and Hashimoto’s thyroiditis, with a 7-10 times higher incidence than in men. The thyroid gland synthesizes iodine-containing hormones, which are needed for several cellular processes in the body. I-131 is routinely used in thyroid cancer treatment, and I-131-containing pharmaceuticals are used for treatment of patients with some neuroendocrine tumor types. This study was performed to evaluate possible differences between sexes in tissue uptake of I-131 in mice. Methods: 35 male and 35 female mice (C57BL/6N, n=5/group) were intravenously injected with I-131 at 8 am, and animals were killed 1 h to 7 d after injection. Tissue samples were collected, weighed, and measured to determine I-131 activity concentration. Results: The results indicate differences in I-131 uptake between males and females, especially in the salivary glands and kidneys. In the majority of the tissues and observed time points, statistical significant differences were found. The decrease of activity concentration in thyroid after 18 h was slower for females (statistical significant), though the obtained maximum uptake was similar. Conclusion: The I-131 uptake differs between males and females, which would result in different absorbed doses from exposure to the same amount of I-131. The difference in magnitude is tissue-dependent. The results suggest biological sex to be treated as a variable in dose calculations and risk assessments when treating cancer patients with radiopharmaceuticals containing I-131.
|
|
| 34. |
- Forsberg, Viviane, 1981-, et al.
(author)
-
Exfoliated MoS2 in Water without Additives
- 2016
-
In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 11:4
-
Journal article (peer-reviewed)abstract
- Many solution processing methods of exfoliation of layered materials have been studied during the last few years; most of them are based on organic solvents or rely on surfactants andother funtionalization agents. Pure water should be an ideal solvent, however, it is generallybelieved, based on solubility theories that stable dispersions of water could not be achievedand systematic studies are lacking. Here we describe the use of water as a solvent and thestabilization process involved therein. We introduce an exfoliation method of molybdenumdisulfide (MoS2) in pure water at high concentration (i.e., 0.14±0.01 g L−1). This was achieved by thinning the bulk MoS2by mechanical exfoliation between sand papers and dis-persing it by liquid exfoliation through probe sonication in water. We observed thin MoS2nanosheets in water characterized by TEM, AFM and SEM images. The dimensions of thenanosheets were around 200 nm, the same range obtained in organic solvents. Electropho-retic mobility measurements indicated that electrical charges may be responsible for the sta-bilization of the dispersions. A probability decay equation was proposed to compare thestability of these dispersions with the ones reported in the literature. Water can be used as asolvent to disperse nanosheets and although the stability of the dispersions may not be ashigh as in organic solvents, the present method could be employed for a number of applications where the dispersions can be produced on site and organic solvents are not desirable.
|
|
| 35. |
- Forsberg, Viviane, 1981-, et al.
(author)
-
Liquid Exfoliation of Layered Materials in Water for Inkjet Printing
- 2016
-
In: Printing for Fabrication 2016. - USA : Curran Associates, Inc.. - 9780892083220 - 9780892083237 - 9780892083213 ; 60:4, s. 1-7
-
Conference paper (peer-reviewed)abstract
- MoS2 is a layered material which is abundant and non-toxic and has been increasingly studied during the last few years as a semiconducting alternative to graphene. While most studies have been performed on single MoS2 nanosheets, for example to demonstrate high-performance electronic transistors, more work is needed to explore the use of MoS2 in printed electronics. The importance of using MoS2 as a printed electronic material could be understood by considering the several orders higher electron mobility in MoS2, even in several nanometer thick layers, compared to the organic and other materials used today. In the few studies performed so far on printing MoS2, the developed dispersions used mainly organic solvents that might be detrimental for the environment. Here, we show an environmentally friendly liquid-based exfoliation method in water where the solution was stabilized by sodium dodecyl sulfate (SDS) surfactant. The dispersions consisted of very thin MoS2 nanosheets with average lateral size of about 150 nm, surface tension of 28 mN m-1 and a shelf life of a year. Although both the concentration and viscosity was less than optimal, we were able to inkjet print the MoS2 solution on paper and on PET films, using multiple printing passes. By tuning the concentration/viscosity, this approach might lead to an environmentally friendly MoS2 ink suitable for printed electronics.
|
|
| 36. |
-
Friluftsliv explored : An environmental and outdoor teaching approach for knowledge, emotions and quality of life
- 2021
-
Editorial collection (other academic/artistic)abstract
- Friluftsliv explored doesn’t only include nature knowledge, techniques in the outdoors and outdoor pedagogics but also covers ecology, human ecology, geography, environmental and societal questions, history, health, biology, craft and lots of practical activities -both for urban and rural friluftsliv. In this translation to English of the revised fifth edition of the Swedish book there are many activities and the text is suitable for the modern day.Friluftsliv embraces the feeling around the campfire, paddling along winding rivers and walking towards the distant blue mountains. But, it is also to whittle a stick, to remember your waterproofs and to find your way home.Knowledge emerges when you combine imagination with facts and the glint in your eyes, using all our outdoor environments: forests, water, the coast, mountains and the nature close at hand.Emotion is to swim in crystal clear water far out in the archipelago and to see the clouds gliding across the sky. But also, to be able to present other sides of yourself, to be fascinated by your own body, the struggling ant and the sight of frost on trees.Quality of life is to experience friluftsliv – as it happens!
|
|
| 37. |
- Hagberg, Catharina, 1949, et al.
(author)
-
Ehlers-Danlos Syndrome (EDS) focusing on oral symptoms: a questionnaire study.
- 2004
-
In: Orthodontics & craniofacial research. - : Wiley. - 1601-6335 .- 1601-6343. ; 7:3, s. 178-85
-
Journal article (peer-reviewed)abstract
- OBJECTIVES: To study the prevalence of oral problems reported among a large group of adults with Ehlers-Danlos Syndrome (EDS). Furthermore, to compare proportions of disorders and general psychological well-being with those in a cohort of randomized population-based controls. DESIGN: A questionnaire study. SETTING AND SAMPLE POPULATION: A total of 265 persons with EDS (response rate 77%) and 750 controls (response rate 63%) in a population-based cohort. Sixty persons were excluded from the EDS group (support members, children, undiagnosed EDS) leaving a final study group of 144 adults. The final control group consisted of 331 persons since many had sent back unanswered questionnaires. EXPERIMENTAL VARIABLES: Questions concerning general and oral symptoms, VAS scales for masticatory muscle pain ratings and General Health Questionnaire (GHQ12) questions were included. OUTCOME MEASURE: Proportions of affirmative answers in EDS group and controls were compared. Mean values for ratings on VAS scales. Proportions of GHQ12 scores with a threshold score of four or more. RESULTS: The proportions of affirmative answers for persons with EDS concerning general health problems, oral problems and masticatory muscle symptoms were significantly higher compared with controls. In the EDS group those who had daily pain in the masticatory muscles had significantly higher mean values on the VAS scales compared with those who had pain a few days per month. A GHQ12 score of four or more was significantly more common in the EDS group than in the controls, suggesting that a decline in psychological well-being was also more common in this group. CONCLUSION: It is important that dental practitioners should be aware of the oral problems associated with EDS and the impact the disease has on quality of life.
|
|
| 38. |
- Håglin, Lena, et al.
(author)
-
Kost och hälsa i Sverige och världen
- 2006
-
In: Näringslära för högskolan. - : Liber AB, Stockholm. - 9147053550 ; , s. 29-57
-
Book chapter (pop. science, debate, etc.)
|
|
| 39. |
- Janson, Veronica, et al.
(author)
-
Acquisition of Cisplatin-resistance in Malignant Mesothelioma Cells Abrogates Na,K(+),2Cl(-;)-cotransport Activity and Cisplatin-induced Early Membrane Blebbing
- 2008
-
In: Cellular Physiology and Biochemistry. - : S. Karger. - 1015-8987 .- 1421-9778. ; 22:1-4, s. 45-56
-
Journal article (peer-reviewed)abstract
- AIMS: Resistance mechanisms are important limiting factors in the treatment of solid malignancies with cis-diamminedichloroplatinum(II) (cisplatin). To gain further understanding of the effects of acquired cisplatin-resistance, we compared a human malignant pleural mesothelioma cell line (p31) to a sub-line (p31res1.2) with acquired cisplatin-resistance.METHODS AND RESULTS: The role of Na(+),K(+),2Cl(-)-cotransport (NKCC1) activity in cisplatin-induced morphological changes and acquired cisplatin-resistance was investigated in a time-resolved manner. Acquisition of cisplatin-resistance resulted in markedly reduced NKCC1 activity, absence of cisplatin-induced early membrane blebbing, and increased basal caspase-3 activity. At equitoxic cisplatin concentrations, P31res1.2 cells had a faster activation of caspase-3 than P31 cells, but the end-stage cytotoxicity and number of cells with DNA fragmentation was similar. Bumetanide inhibition of NKCC1 activity in P31 cells repressed cisplatin-induced early-phase membrane blebbing but did not increase P31 cell resistance to cisplatin.CONCLUSIONS: Together, these results suggest that active NKCC1 was necessary for cisplatin-induced early membrane blebbing of P31 cells, but not for cisplatin-resistance. Thus, acquisition of cisplatin-resistance can affect mechanisms that have profound effects on cisplatin-induced morphological changes but are not necessary for the subsequent progression to apoptosis.
|
|
| 40. |
- Kunzmann, Andrea, et al.
(author)
-
Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells
- 2011
-
In: Toxicology and Applied Pharmacology. - : Elsevier BV. - 0041-008X .- 1096-0333. ; 253:2, s. 81-93
-
Journal article (peer-reviewed)abstract
- Engineered nanoparticles are being considered for a wide range of biomedical applications, from magnetic resonance imaging to "smart" drug delivery systems. The development of novel nanomaterials for biomedical applications must be accompanied by careful scrutiny of their biocompatibility. In this regard, particular attention should be paid to the possible interactions between nanoparticles and cells of the immune system, our primary defense system against foreign invasion. On the other hand, labeling of immune cells serves as an ideal tool for visualization, diagnosis or treatment of inflammatory processes, which requires the efficient internalization of the nanoparticles into the cells of interest. Here, we compare novel monodispersed silica-coated iron oxide nanoparticles with commercially available dextran-coated iron oxide nanoparticles. The silica-coated iron oxide nanoparticles displayed excellent magnetic properties. Furthermore, they were nontoxic to primary human monocyte-derived macrophages at all doses tested whereas dose-dependent toxicity of the smaller silica-coated nanoparticles (30 nm and 50 nm) was observed for primary monocyte-derived dendritic cells, but not for the similarly small dextran-coated iron oxide nanoparticles. No macrophage or dendritic cell secretion of pro-inflammatory cytokines was observed upon administration of nanoparticles. The silica-coated iron oxide nanoparticles were taken up to a significantly higher degree when compared to the dextran-coated nanoparticles, irrespective of size. Cellular internalization of the silica-coated nanoparticles was through an active, actin cytoskeleton-dependent process. We conclude that these novel silica-coated iron oxide nanoparticles are promising materials for medical imaging, cell tracking and other biomedical applications. (C) 2011 Elsevier Inc. All rights reserved.
|
|
| 41. |
- Kyhse-Andersen, Jan, et al.
(author)
-
Serum cystatin C, determined by a rapid, automated particle-enhanced turbidimetric method, is a better marker than serum creatinine for glomerular filtration rate
- 1994
-
In: Clinical Chemistry. - 0009-9147. ; 40:10, s. 1921-1926
-
Journal article (peer-reviewed)abstract
- We describe a fully automated particle-enhanced turbidimetric assay for cystatin C in undiluted serum and EDTA-plasma. The throughput is 90 samples per hour and urgent samples can be analyzed in 7 min. The assay range (0.4-14.1 mg/L) covers the concentration range in health and disease. The within- and between-run imprecision is 0.9% and 2.2%, respectively. Analytical recovery of additions of recombinant cystatin C averaged 98%. Rheumatoid factors (< or = 323,000 IU/L), bilirubin (< or = 150 mumol/L), hemoglobin (< or = 1.2 g/L), and triglycerides (< or = 8.5 mmol/L) do not interfere in the assay. In view of the superior (by ROC analysis) diagnostic accuracy of serum concentrations of cystatin C for reduced glomerular filtration rate (GFR) in comparison with creatinine, cystatin C seems an attractive alternative to creatinine for estimation of GFR.
|
|
| 42. |
- Larsson, Anna-Britta, et al.
(author)
-
Klinisk slutexamination i tandhygienistprogrammet vid fyra lärosäten
- 2009
-
Reports (pop. science, debate, etc.)abstract
- Den legitimerade tandhygienisten har en nyckelroll inom promotion och prevention i svensk tandvård. I arbetet som tandhygienist krävs att kunna arbeta självständigt och ta väl underbyggda beslut för att ge en god och säker vård. Syftet med projektet var att utveckla, pröva och utvärdera en modell för klinisk slutexamination utifrån de krav som ställs för att arbeta som legitimerad tandhygienist. Projektarbetet resulterade i en modell bestående av en teoretisk och en klinisk examination. Den teoretiska examinationen består av ett fiktivt patientfall medan den kliniska examinationen omfattar en realistisk patientsituation där studenten omhändertar och behandlar en patient. Den framtagna modellens styrka är att samtliga studenter vid de olika lärosätena examineras kliniskt efter samma bedömningsgrunder vilket borgar för en god och jämförbar kvalitet.
|
|
| 43. |
- Levin, Britta, et al.
(author)
-
Memory performance during G exposure as assessed by a word recognition task
- 2007
-
In: Aviation, Space and Environmental Medicine. - 0095-6562 .- 1943-4448. ; 78:6, s. 587-592
-
Journal article (peer-reviewed)abstract
- Background: Pilots of modern fighter aircraft are exposed to substantial physiological and mental stressors. The objective of this study was to investigate how memory performance, in terms of encoding and/or retrieval processes, was affected by sustained +Gz exposure. Method: There were 18 healthy men ranging from experienced fighter pilots to novice riders who participated. A word continuous recognition task (CRT) was employed as a memory test. The task consisted of three consecutive phases: 1) encoding of familiar words at 1 G, 2) encoding and retrieval of words at 70% of the subject's relaxed G-tolerance level, equivalent to +3.7 ± 0.54 Gz, and 3) encoding and retrieval of words at 1 G. In addition, each subject performed the CRT in a 1-G-only control condition. Physiological and psycho-physiological measures included continuous monitoring of ECG, arterial oxygen saturation, arterial BP at head level, and response time. Results: Data analysis showed that the capability to recognize words encoded at 1 G did not differ between conditions, indicating that the retrieval process was insensitive to increased Gz load. However, the ability to recognize words previously encoded during G exposure was reduced by approximately 10% as compared with control. Since the analysis revealed that the words were perceived, this result suggests that the encoding process was impaired in hypergravity. Conclusion: The results indicate that memory encoding, but not retrieval, was affected negatively when exposed to substantial and sustained +Gz loads. Copyright © by Aerospace Medical Association.
|
|
| 44. |
- Lindmark, Lindmark, 1965-, et al.
(author)
-
Klinisk slutexamination i tandhygienistprogrammet vid fyra lärosäten
- 2009
-
Reports (pop. science, debate, etc.)abstract
- Den legitimerade tandhygienisten har en nyckelroll inom promotion och prevention i svensk tandvård. I arbetet som tandhygienist krävs att kunna arbeta självständigt och ta väl underbyggda beslut för att ge en god och säker vård. Syftet med projektet var att utveckla, pröva och utvärdera en modell för klinisk slutexamination utifrån de krav som ställs för att arbeta som legitimerad tandhygienist. Projektarbetet resulterade i en modell bestående av en teoretisk och en klinisk examination. Den teoretiska examinationen består av ett fiktivt patientfall medan den kliniska examinationen omfattar en realistisk patientsituation där studenten omhändertar och behandlar en patient. Den framtagna modellens styrka är att samtliga studenter vid de olika lärosätena examineras kliniskt efter samma bedömningsgrunder vilket borgar för en god och jämförbar kvalitet.
|
|
| 45. |
- Lodin, Karin, et al.
(author)
-
Patient global assessment and inflammatory markers in patients with idiopathic inflammatory myopathies : A longitudinal study
- 2024
-
In: Seminars in Arthritis & Rheumatism. - : Elsevier. - 0049-0172 .- 1532-866X. ; 65
-
Journal article (peer-reviewed)abstract
- AimTo explore if patient global assessment (PGA) is associated with inflammation over time and if associations are explained by other measures of disease activity and function in patients with idiopathic inflammatory myopathies (IIM).MethodsPGA and systemic inflammatory markers prospectively collected over five years were retrieved from the International MyoNet registry for 1200 patients with IIM. Associations between PGA, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and creatine kinase (CK) were analyzed using mixed models. Mediation analysis was used to test if the association between PGA and inflammatory markers during the first year of observation could be explained by measures of disease activity and function.ResultsPGA improved, and inflammatory markers decreased during the first year of observation. In the mixed models, high levels of inflammatory markers were associated with worse PGA in both men and women across time points during five years of observation. In men, but not in women, the association between elevated ESR, CRP and poorer PGA was explained by measures of function and disease activity. With a few exceptions, the association between improved PGA and reduced inflammatory markers was partially mediated by improvements in all measures of function and disease activity.ConclusionIncreased levels of systemic inflammation are associated with poorer PGA in patients with IIM. In addition to known benefits of lowered inflammation, these findings emphasize the need to reduce systemic inflammation to improve subjective health in patients with IIM. Furthermore, the results demonstrate the importance of incorporating PGA as an outcome measure in clinical practice and clinical trials.
|
|
| 46. |
- Loisel, Julie, et al.
(author)
-
A database and synthesis of northern peatland soil properties and Holocene carbon and nitrogen accumulation
- 2014
-
In: The Holocene. - : SAGE Publications. - 0959-6836 .- 1477-0911. ; 24:9, s. 1028-1042
-
Journal article (peer-reviewed)abstract
- Here, we present results from the most comprehensive compilation of Holocene peat soil properties with associated carbon and nitrogen accumulation rates for northern peatlands. Our database consists of 268 peat cores from 215 sites located north of 45 degrees N. It encompasses regions within which peat carbon data have only recently become available, such as the West Siberia Lowlands, the Hudson Bay Lowlands, Kamchatka in Far East Russia, and the Tibetan Plateau. For all northern peatlands, carbon content in organic matter was estimated at 42 +/- 3% (standard deviation) for Sphagnum peat, 51 +/- 2% for non-Sphagnum peat, and at 49 +/- 2% overall. Dry bulk density averaged 0.12 +/- 0.07 g/cm(3), organic matter bulk density averaged 0.11 +/- 0.05 g/cm(3), and total carbon content in peat averaged 47 +/- 6%. In general, large differences were found between Sphagnum and non-Sphagnum peat types in terms of peat properties. Time-weighted peat carbon accumulation rates averaged 23 +/- 2 (standard error of mean) g C/m(2)/yr during the Holocene on the basis of 151 peat cores from 127 sites, with the highest rates of carbon accumulation (25-28 g C/m(2)/yr) recorded during the early Holocene when the climate was warmer than the present. Furthermore, we estimate the northern peatland carbon and nitrogen pools at 436 and 10 gigatons, respectively. The database is publicly available at https://peatlands.lehigh.edu.
|
|
| 47. |
|
|
| 48. |
- Lötstedt, Britta
(author)
-
Spatial mapping of bacteria and transcriptomes
- 2022
-
Doctoral thesis (other academic/artistic)abstract
- Novel insights into biological functions and mechanisms, cell networks and evolutionary relationships are gained through development of sequencing technologies and sequencing based applications. Massively parallel sequencing has enabled analysis of big data at gene and protein expression levels, but has also characterized bacterial communities. Additionally, different technological advancements enabled us to track those expression changes in single cells, to reveal insights into rare cell populations, or with added spatial resolution, to explore highly complex environments such as tissues. This thesis gives an overview of different technical, biological and computational methods used in genomics today with a specific focus on spatial techniques for detailed tissue characterization. This is followed by a chapter summarizing recent scientific contributions made by the author that have been included as part of this thesis. In Paper I, 16S sequencing was used to study the diversity and composition of bacterial communities with specific focus on the aerodigestive microbiome in children who had undergone a lung transplant. Potential connections between the microbiome and irregular gastric muscle movements were also examined. Patients with a lung transplant had significantly lower microbial diversity in the gastric and oropharyngeal sites as compared to controls, however, lung transplant recipients showed similar bacterial compositions, independent of motility status. Samples in the lung transplant patient group were in general dominated by Staphylococcaceae but Streptococcus, Prevotella and Veillonella were common in the gastric and oropharyngeal samples. Next, an automated method for simultaneous spatial analysis of both gene and antibodybased protein expression in tissue sections, named SM-Omics, was developed in Paper II. SM-Omics enabled simultaneous detection of proteins, by using either immunofluorescence or DNAbarcoded antibodies, and analysis of the spatial transcriptome in the same tissue section. SM-Omics was applied to the mouse brain and spleen and obtained correlated spatial patterns between respective gene and antibody measurements. The method allowed processing of up to 64 in situ spatial reactions or up to 96 sequencing-ready libraries, of high complexity, in a ~2 days process. The spatial host-microbiome sequencing method, presented in Paper III, was used to concurrently study the spatial environment created between bacteria and host cells within a tissue section. Using spatial host-microbiome sequencing, colonic sections from three different mouse models were examined by simultaneous in situ capture of both mRNA and 16S sequences, followed by sequencing and taxonomic assignment of bacterial 16S sequences using a deep learning model. ~17,000 genes and 39 bacteria genera across 16 different morphological regions were quantitatively assessed in the mouse colon. We reported specific genera in the interfold and lumen regions of the colon, as well as spatially variable genes across 100 tissue sections. To better understand genotype-relevant changes impacted by bacterial presence, we defined cell-type specific interactions described with sets of activated pathways. Finally, consecutive tissue sections of multiple synovial biopsies from patients suffering from rheumatoid arthritis were processed using the Spatial Transcriptomics method and sequenced in Paper IV. The alignment and transformation of the consecutive tissue sections enabled spatial profiling in 3D of genes and cell types within the biopsies. Spatially variable gene expression patterns revealed clusters radially distributed around organized structures of infiltrating leukocytes (TLOs). In patients with developed TLOs, these structures contained proinflammatory B cells, while the surrounding areas were high in fibroblasts.
|
|
| 49. |
|
|
| 50. |
- Mladenovic, Zivko, 1977-, et al.
(author)
-
In vitro study of the biological interface of Bio-Oss : implications of the experimental setup
- 2013
-
In: Clinical Oral Implants Research. - : John Wiley & Sons. - 0905-7161 .- 1600-0501. ; 24:3, s. 329-335
-
Journal article (peer-reviewed)abstract
- Objectives To systematically investigate the biological interface of Bio-Oss by analysing dissolution–precipitation behaviour and osteogenic responses using in vitro experimental systems.Material and methods Different concentrations (1–100 mg/ml) of Bio-Oss were incubated in cell culture medium for 24 h before elemental concentrations for calcium, phosphorus and silicon in the medium were analysed with inductive coupled plasma-optical emission spectroscopy. Radioactive calcium-45 isotope labelling technique was used to study possible precipitation of calcium on the Bio-Oss particle. Biological interface of Bio-Oss was studied in osteogenic experiments using mineralization medium and three different sources of cells (primary mouse bone marrow stromal cells, primary rat calvarial cells and MC3T3-E1 mouse pre-osteoblast cell line). Cells were fixed and stained with Toulidine blue, von Kossa or Alizarin Red staining for confirmation of extracellular matrix mineralization.Results Elemental analysis of the cell culture medium demonstrated a significant decrease of calcium and phosphorus and a dose-dependent release of silicon to the medium after incubation with Bio-Oss. A significant decrease of calcium and phosphorus in the medium occurred even at low concentrations of Bio-Oss. Uptake of calcium on the Bio-Oss particle was confirmed with radioactive calcium-45 isotope labelling technique. In osteogenic experiments with Bio-Oss (<1 mg/ml), matrix mineralization around the Bio-Oss particles were demonstrated in all three cell types with von Kossa and Alizarin Red staining.Conclusion Dissolution–precipitation reactions occur at the surface of Bio-Oss, and osteogenic responses are seen at the biological interface. The concentration of Bio-Oss is a key factor for the experimental in vitro results, and may also have implications for the clinic.
|
|
