SwePub - search: WFRF:(Arseniev Anatolii)

Enumeration	Reference	Cover	Find
1.	Sabantsev, Anton, et al. (author) Spatiotemporally controlled generation of NTPs for single-molecule studies 2022 In: Nature Chemical Biology. - : Springer Nature. - 1552-4450 .- 1552-4469. ; 18:10, s. 1144- Journal article (peer-reviewed)abstract Many essential processes in the cell depend on proteins that use nucleoside triphosphates (NTPs). Methods that directly monitor the often-complex dynamics of these proteins at the single-molecule level have helped to uncover their mechanisms of action. However, the measurement throughput is typically limited for NTP-utilizing reactions, and the quantitative dissection of complex dynamics over multiple sequential turnovers remains challenging. Here we present a method for controlling NTP-driven reactions in single-molecule experiments via the local generation of NTPs (LAGOON) that markedly increases the measurement throughput and enables single-turnover observations. We demonstrate the effectiveness of LAGOON in single-molecule fluorescence and force spectroscopy assays by monitoring DNA unwinding, nucleosome sliding and RNA polymerase elongation. LAGOON can be readily integrated with many single-molecule techniques, and we anticipate that it will facilitate studies of a wide range of crucial NTP-driven processes.

Sabantsev, Anton, et al. (author)
Spatiotemporally controlled generation of NTPs for single-molecule studies
2022
In: Nature Chemical Biology. - : Springer Nature. - 1552-4450 .- 1552-4469. ; 18:10, s. 1144-
Journal article (peer-reviewed)abstract
- Many essential processes in the cell depend on proteins that use nucleoside triphosphates (NTPs). Methods that directly monitor the often-complex dynamics of these proteins at the single-molecule level have helped to uncover their mechanisms of action. However, the measurement throughput is typically limited for NTP-utilizing reactions, and the quantitative dissection of complex dynamics over multiple sequential turnovers remains challenging. Here we present a method for controlling NTP-driven reactions in single-molecule experiments via the local generation of NTPs (LAGOON) that markedly increases the measurement throughput and enables single-turnover observations. We demonstrate the effectiveness of LAGOON in single-molecule fluorescence and force spectroscopy assays by monitoring DNA unwinding, nucleosome sliding and RNA polymerase elongation. LAGOON can be readily integrated with many single-molecule techniques, and we anticipate that it will facilitate studies of a wide range of crucial NTP-driven processes.

Träfflista för sökning "WFRF:(Arseniev Anatolii) "