| 1. |
- Minekus, M., et al.
(author)
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A standardised static in vitro digestion method suitable for food - an international consensus
- 2014
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In: Food and Function. - : Royal Society of Chemistry (RSC). - 2042-6496 .- 2042-650X. ; 5:6, s. 1113-1124
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Journal article (peer-reviewed)abstract
- Simulated gastro-intestinal digestion is widely employed in many fields of food and nutritional sciences, as conducting human trials are often costly, resource intensive, and ethically disputable. As a consequence, in vitro alternatives that determine endpoints such as the bioaccessibility of nutrients and non-nutrients or the digestibility of macronutrients (e. g. lipids, proteins and carbohydrates) are used for screening and building new hypotheses. Various digestion models have been proposed, often impeding the possibility to compare results across research teams. For example, a large variety of enzymes from different sources such as of porcine, rabbit or human origin have been used, differing in their activity and characterization. Differences in pH, mineral type, ionic strength and digestion time, which alter enzyme activity and other phenomena, may also considerably alter results. Other parameters such as the presence of phospholipids, individual enzymes such as gastric lipase and digestive emulsifiers vs. their mixtures (e. g. pancreatin and bile salts), and the ratio of food bolus to digestive fluids, have also been discussed at length. In the present consensus paper, within the COST Infogest network, we propose a general standardised and practical static digestion method based on physiologically relevant conditions that can be applied for various endpoints, which may be amended to accommodate further specific requirements. A frameset of parameters including the oral, gastric and small intestinal digestion are outlined and their relevance discussed in relation to available in vivo data and enzymes. This consensus paper will give a detailed protocol and a line-by-line, guidance, recommendations and justifications but also limitation of the proposed model. This harmonised static, in vitro digestion method for food should aid the production of more comparable data in the future.
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| 2. |
- Fogle, M., et al.
(author)
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Electron-impact ionization of Be-like CIII, NIV, and OV
- 2008
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In: Astrophysical Journal Supplement Series. - : American Astronomical Society. - 0067-0049 .- 1538-4365. ; 175:2, s. 543-556
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Journal article (peer-reviewed)abstract
- We present recent measurements of absolute electron-impact ionization cross sections for Be-like C III, N IV, and O V forming Li- like C IV, N V, and O VI. The measurements were taken using the crossed-beams apparatus at Oak Ridge National Laboratory. A gas cell beam attenuation method was used to independently measure the metastable fractions present in the ion beams. The measured ionization cross sections were compared with calculations using the R-matrix with pseudostates and distorted-wave theoretical methods. Best agreement is found with the R-matrix with pseudostates cross sections results that account for the metastable fractions inferred from the gas attenuation measurements. We present a set of recommended rate coefficients for electron-impact single ionization from the ground state and metastable term of each ion.
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| 3. |
- Shingles, L. J., et al.
(author)
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Monte Carlo radiative transfer for the nebular phase of Type Ia supernovae
- 2020
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In: Monthly notices of the Royal Astronomical Society. - : OXFORD UNIV PRESS. - 0035-8711 .- 1365-2966. ; 492:2, s. 2029-2043
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Journal article (peer-reviewed)abstract
- We extend the range of validity of the ARTIS 3D radiative transfer code up to hundreds of days after explosion, when Type Ia supernovae (SNe Ia) are in their nebular phase. To achieve this, we add a non-local thermodynamic equilibrium population and ionization solver, a new multifrequency radiation field model, and a new atomic data set with forbidden transitions. We treat collisions with non-thermal leptons resulting from nuclear decays to account for their contribution to excitation, ionization, and heating. We validate our method with a variety of tests including comparing our synthetic nebular spectra for the well-known one-dimensional W7 model with the results of other studies. As an illustrative application of the code, we present synthetic nebular spectra for the detonation of a sub-Chandrasekhar white dwarf (WD) in which the possible effects of gravitational settling of Ne-22 prior to explosion have been explored. Specifically, we compare synthetic nebular spectra for a 1.06 M-circle dot WD model obtained when 5.5 Gyr of very efficient settling is assumed to a similar model without settling. We find that this degree of Ne-22 settling has only amodest effect on the resulting nebular spectra due to increased Ni-58 abundance. Due to the high ionization in sub-Chandrasekhar models, the nebular [Ni II] emission remains negligible, while the [Ni III] line strengths are increased and the overall ionization balance is slightly lowered in the model with Ne-22 settling. In common with previous studies of sub-Chandrasekhar models at nebular epochs, these models overproduce [Fe III] emission relative to [Fe II] in comparison to observations of normal SNe Ia.
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| 4. |
- Brodkorb, A., et al.
(author)
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INFOGEST static in vitro simulation of gastrointestinal food digestion
- 2019
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In: Nature Protocols. - : Springer Science and Business Media LLC. - 1754-2189 .- 1750-2799. ; 14:4, s. 991-1014
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Journal article (peer-reviewed)abstract
- Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.
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| 5. |
- Loch, S.D., et al.
(author)
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Influence of long-lived metastable levels on the electron-impact single ionization of C2
- 2005
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In: Physical Review A. Atomic, Molecular, and Optical Physics. - 1050-2947 .- 1094-1622. ; 71:1, s. 012716-1
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Journal article (peer-reviewed)abstract
- A joint theoretical and experimental investigation is made of the influence of long-lived metastable levels on the electron-impact single ionization of C2+. It is expected that our electron cyclotron resonance ion source produces a beam with 40% of the C2+ ions in the 1s(2)2s(2) S-1(0) ground level and 60% in the 1s(2)2s2p P-3(0,2) excited levels. The comparison of nonperturbative close-coupling calculations with previous single-pass crossed beams and with our multiple-pass storage-ring measurements for the electron-impact ionization of C2+ is consistent with the predicted large metastable fraction. Reasonable agreement is found between the present time-dependent close-coupling, R-matrix with pseudostates, and converged-close-coupling ionization cross-section calculations for the ground and first excited configurations and experimental measurement, assuming a 60% metastable fraction in the ion beam. Distorted-wave calculations are found to overestimate the ionization cross section from both the ground and metastable terms, compared with nonperturbative calculations, resulting in an overestimation of the resultant total cross section when compared with experiment. It is clear that collisional-radiative modeling of the evolution of atomic plasmas through the Be-like ionization stage will need to take into account the role of both ground and metastable levels.
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