| 1. |
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| 2. |
- Serge, M. A., et al.
(author)
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Testing the Effect of Relative Pollen Productivity on the REVEALS Model : A Validated Reconstruction of Europe-Wide Holocene Vegetation
- 2023
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In: Land. - : MDPI. - 2073-445X. ; 12:5
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Journal article (peer-reviewed)abstract
- Reliable quantitative vegetation reconstructions for Europe during the Holocene are crucial to improving our understanding of landscape dynamics, making it possible to assess the past effects of environmental variables and land-use change on ecosystems and biodiversity, and mitigating their effects in the future. We present here the most spatially extensive and temporally continuous pollen-based reconstructions of plant cover in Europe (at a spatial resolution of 1 degrees x 1 degrees) over the Holocene (last 11.7 ka BP) using the 'Regional Estimates of VEgetation Abundance from Large Sites' (REVEALS) model. This study has three main aims. First, to present the most accurate and reliable generation of REVEALS reconstructions across Europe so far. This has been achieved by including a larger number of pollen records compared to former analyses, in particular from the Mediterranean area. Second, to discuss methodological issues in the quantification of past land cover by using alternative datasets of relative pollen productivities (RPPs), one of the key input parameters of REVEALS, to test model sensitivity. Finally, to validate our reconstructions with the global forest change dataset. The results suggest that the RPPs.st1 (31 taxa) dataset is best suited to producing regional vegetation cover estimates for Europe. These reconstructions offer a long-term perspective providing unique possibilities to explore spatial-temporal changes in past land cover and biodiversity.
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| 3. |
- Gunton, L. M., et al.
(author)
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Annelids of the eastern Australian abyss collected by the 2017 RV 'Investigator' voyage
- 2021
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In: Zookeys. - : Pensoft Publishers. - 1313-2989 .- 1313-2970. ; :1020, s. 1-198
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Journal article (peer-reviewed)abstract
- In Australia, the deep-water (bathyal and abyssal) benthic invertebrate fauna is poorly known in comparison with that of shallow (subtidal and shelf) habitats. Benthic fauna from the deep eastern Australian margin was sampled systematically for the first time during 2017 RV 'Investigator' voyage 'Sampling the Abyss'. Box core, Brenke sledge, and beam trawl samples were collected at one-degree intervals from Tasmania, 42 degrees S, to southern Queensland, 24 degrees S, from 900 to 4800 m depth. Annelids collected were identified by taxonomic experts on individual families around the world. A complete list of all identified species is presented, accompanied with brief morphological diagnoses, taxonomic remarks, and colour images. A total of more than 6000 annelid specimens consisting of 50 families (47 Polychaeta, one Echiura, two Sipuncula) and 214 species were recovered. Twenty-seven species were given valid names, 45 were assigned the qualifier cf., 87 the qualifier sp., and 55 species were considered new to science. Geographical ranges of 16 morphospecies extended along the eastern Australian margin to the Great Australian Bight, South Australia; however, these ranges need to be confirmed with genetic data. This work providing critical baseline biodiversity data on an important group of benthic invertebrates from a virtually unknown region of the world's ocean will act as a springboard for future taxonomic and biogeographic studies in the area.
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| 4. |
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| 5. |
- Austin, K., et al.
(author)
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Serum neurofilament light concentration does not increase following exposure to low velocity football heading
- 2021
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In: Science and Medicine in Football. - : Informa UK Limited. - 2473-3938 .- 2473-4446. ; 5:3, s. 188-194
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Journal article (peer-reviewed)abstract
- Objectives: To investigate if heading frequency and impact biomechanics in a single session influence the concentration of serum neurofilament light (NF-L), a sensitive biomarker for axonal damage, up to 7 days after heading incident at ball velocities reflecting basic training drills. Methods: Forty-four males were randomized into either control (n = 8), 10 header (n = 12), 20 header (n = 12) or 40 header (n = 12) groups. Linear and angular head accelerations were quantified during heading. Venous blood samples were taken at baseline, 6 h, 24 h and 7 days after heading. Serum NF-L was quantified using Quanterix NF-L assay kit on the Simoa HD-1 Platform. Results: Serum NF-L did not alter over time (p = 0.44) and was not influenced by number of headers [p = 0.47; mean (95% CI) concentrations at baseline 6.00 pg center dot ml(-) (1) (5.00-7.00 pg center dot ml(-) (1)); 6 h post 6.50 pg center dot ml(-1) (5.70-7.29 pg center dot ml(-1)); 24 h post 6.07 pg center dot ml(-1) (5.14-7.01 pg center dot ml(-) (1)); and 7 days post 6.46 pg center dot ml(-1) (5.45-7.46 pg center dot ml(-1))]. There was no relationship between percentage change in NF-L and summed session linear and angular head accelerations. Conclusion: In adult men, heading frequency or impact biomechanics did not affect NF-L response during a single session of headers at ball velocities reflective of basic training tasks.
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| 6. |
- Blagoveshchenskaya, N.F., Kornienko, V.A., Borisova, T.D., Thidé, B., Kosch, M.J., Rietveld, M.T., Mishin, E.V., Luk'yanova, R.Y. and Troshichev, O.A.
(author)
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Ionospheric HF pump wave triggering of local auroral activation.
- 2001
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In: Journal of Geophysical Research. ; 106, s. 29071-29089
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Journal article (peer-reviewed)abstract
- Experimental results from ionospheric HF pumping experiments in the nightside auroral E region above Tromso are reported. We found intriguing evidence that a modification of the ionosphere-magnetosphere coupling, due to the effects of powerful HF waves be
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| 7. |
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| 8. |
- Blagoveshchenskaya, N. F., et al.
(author)
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Heater-induced phenomena in a coupled ionosphere-magnetosphere system
- 2006
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In: Advances in Space Research. - : Elsevier BV. - 0273-1177 .- 1879-1948. ; 38:11, s. 2495-2502
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Journal article (peer-reviewed)abstract
- Experimental results from HF pumping experiments in the nightside auroral E and F region are reported. The experiments were carried out by the use of the EISCAT HF heating facility located near Tromso, Norway, allowing HF pumping the ionosphere in a near magnetic field-aligned direction. We present experimental results from multi-instrument observations related to heater-induced phenomena in a coupled ionosphere-magnetosphere system. The following results have been observed on different occasions: a reverberation effect in scattered signals observed simultaneously on two diagnostic paths which is an indication of Alfven wave generation. This phenomenon was seen under specific disturbed background geophysical conditions, namely, a high electron density in the F region up to 8 MHz produced by soft electron precipitation from the magnetosphere along with low electron density in lower ionosphere; increased ionospheric electric fields; ion outflows from the ionosphere. On another occasion a magnetospheric response to heater turning on and off was found from magnetic pulsation observations over a frequency range up to 5 Hz (the upper frequency limit of the sensitive magnetometer at Kilpisjarvi, located near Tromso). The response manifests itself about 1 min after the heater is turned on and off. Other results have shown the modification of a natural auroral arc and local spiral-like formation. It is thought that a local heater-driven current system is formed. An interesting feature is the generation of the heater-induced ion outflows from the ionosphere. They are observed in night hours under both quiet and disturbed magnetic conditions.
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| 9. |
- Borisova, Elena, et al.
(author)
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Micrometer-resolution X-ray tomographic full-volume reconstruction of an intact post-mortem juvenile rat lung
- 2021
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In: Histochemistry and Cell Biology. - : Springer. - 0948-6143 .- 1432-119X. ; 155, s. 215-226
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Journal article (peer-reviewed)abstract
- In this article, we present an X-ray tomographic imaging method that is well suited for pulmonary disease studies in animal models to resolve the full pathway from gas intake to gas exchange. Current state-of-the-art synchrotron-based tomographic phase-contrast imaging methods allow for three-dimensional microscopic imaging data to be acquired non-destructively in scan times of the order of seconds with good soft tissue contrast. However, when studying multi-scale hierarchically structured objects, such as the mammalian lung, the overall sample size typically exceeds the field of view illuminated by the X-rays in a single scan and the necessity for achieving a high spatial resolution conflicts with the need to image the whole sample. Several image stitching and calibration techniques to achieve extended high-resolution fields of view have been reported, but those approaches tend to fail when imaging non-stable samples, thus precluding tomographic measurements of large biological samples, which are prone to degradation and motion during extended scan times. In this work, we demonstrate a full-volume three-dimensional reconstruction of an intact rat lung under immediate post-mortem conditions and at an isotropic voxel size of (2.75 mu m)(3). We present the methodology for collecting multiple local tomographies with 360 degrees extended field of view scans followed by locally non-rigid volumetric stitching. Applied to the lung, it allows to resolve the entire pulmonary structure from the trachea down to the parenchyma in a single dataset. The complete dataset is available online ().
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| 10. |
- Burkhard, Benjamin, et al.
(author)
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Mapping and assessing ecosystem services in the EU - Lessons learned from the ESMERALDA approach of integration
- 2018
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In: One Ecosystem. - : Pensoft Publishers. - 2367-8194. ; 3
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Journal article (peer-reviewed)abstract
- The European Union (EU) Horizon 2020 Coordination and Support Action ESMERALDA aimed at developing guidance and a flexible methodology for Mapping and Assessment of Ecosystems and their Services (MAES) to support the EU member states in the implementation of the EU Biodiversity Strategy’s Target 2 Action 5. ESMERALDA’s key tasks included network creation, stakeholder engagement, enhancing ecosystem services mapping and assessment methods across various spatial scales and value domains, work in case studies and support of EU member states in MAES implementation. Thus ESMERALDA aimed at integrating various project outcomes around four major strands: i) Networking, ii) Policy, iii) Research and iv) Application. The objective was to provide guidance for integrated ecosystem service mapping and assessment that can be used for sustainable decision-making in policy, business, society, practice and science at EU, national and regional levels. This article presents the overall ESMERALDA approach of integrating the above-mentioned project components and outcomes and provides an overview of how the enhanced methods were applied and how they can be used to support MAES implementation in the EU member states. Experiences with implementing such a large pan-European Coordination and Support Action in the context of EU policy are discussed and recommendations for future actions are given.
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| 11. |
- Geneletti, Davide, et al.
(author)
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Ecosystem services mapping and assessment for policy- and decision-making : Lessons learned from a comparative analysis of European case studies
- 2020
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In: One Ecosystem. - : Pensoft Publishers. - 2367-8194. ; 5, s. e53111-
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Journal article (peer-reviewed)abstract
- This paper analyses and compares a set of case studies on ecosystem services (ES) mapping and assessment with the purpose of formulating lessons learned and recommendations. Fourteen case studies were selected during the EU Horizon 2020 “Coordination and Support Action” ESMERALDA to represent different policy- and decision-making processes throughout the European Union, across a wide range of themes, biomes and scales. The analysis is based on a framework that addresses the key steps of an ES mapping and assessment process, namely policy questions, stakeholder identification and involvement, application of mapping and assessment methods, dissemination and communication and implementation. The analysis revealed that most case studies were policy-orientated or gave explicit suggestions for policy implementation in different contexts, including urban, rural and natural areas. Amongst the findings, the importance of starting stakeholder engagement early in the process was confirmed in order to generate interest and confidence in the project and to increase their willingness to cooperate. Concerning mapping and assessment methods, it was found that the integration of methods and results is essential for providing a comprehensive overview from different perspectives (e.g. social, economic). Finally, lessons learned for effective implementation of ES mapping and assessment results are presented and discussed.
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| 12. |
- Heywood, W. E., et al.
(author)
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Cerebrospinal fluid neurofilament light chain levels in CLN2 disease patients treated with enzyme replacement therapy normalise after two years on treatment
- 2022
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In: F1000Research. - : F1000 Research Ltd. - 2046-1402. ; 10
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Journal article (peer-reviewed)abstract
- Classic late infantile neuronal ceroid lipofuscinosis (CLN2 disease) is caused by a deficiency of tripeptidyl-peptidase-1. In 2017, the first CLN2 enzyme replacement therapy (ERT) cerliponase alfa (Brineura) was approved by the FDA and EMA. The CLN2 disease clinical rating scale (CLN2 CRS) was developed to monitor loss of motor function, language and vision as well as frequency of generalised tonic clonic seizures. Using CLN2 CRS in an open label clinical trial it was shown that Brineura slowed down the progression of CLN2 symptoms. Neurofilament light chain (NfL) is a protein highly expressed in myelinated axons. An increase of cerebrospinal fluid (CSF) and blood NfL is found in a variety of neuroinflammatory, neurodegenerative, traumatic, and cerebrovascular diseases. We analysed CSF NfL in CLN2 patients treated with Brineura to establish whether it can be used as a possible biomarker of response to therapy. Newly diagnosed patients had CSF samples collected and analysed at first treatment dose and up to 12 weeks post-treatment to look at acute changes. Patients on a compassionate use programme who were already receiving ERT for approximately 1yr had CSF samples collected and NfL analysed over the following 1.3 years (2.3 years post-initiation of ERT) to look at long-term changes. All newly diagnosed patients we investigated with classical late infantile phenotype had high NfL levels >2000 pg/ml at start of treatment. No significant change was observed in NfL up to 12 weeks post-treatment. After one year of ERT, two out of six patients still had high NfL levels, but all patients showed a continued decrease, and all had low NfL levels after two years on ERT. NfL levels appear to correspond and predict improved clinical status of patients on ERT and could be useful as a biomarker to monitor neurodegeneration and verify disease modification in CLN2 patients on ERT. © 2022 Iwan K et al.
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| 13. |
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| 14. |
- Pearce, Elena A., et al.
(author)
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Substantial light woodland and open vegetation characterized the temperate forest biome before Homo sapiens
- 2023
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In: Science Advances. - 2375-2548. ; 9:45
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Journal article (peer-reviewed)abstract
- The extent of vegetation openness in past European landscapes is widely debated. In particular, the temperate forest biome has traditionally been defined as dense, closed-canopy forest; however, some argue that large herbivores maintained greater openness or even wood-pasture conditions. Here, we address this question for the Last Interglacial period (129,000–116,000 years ago), before Homo sapiens–linked megafauna declines and anthropogenic landscape transformation. We applied the vegetation reconstruction method REVEALS to 96 Last Interglacial pollen records. We found that light woodland and open vegetation represented, on average, more than 50% cover during this period. The degree of openness was highly variable and only partially linked to climatic factors, indicating the importance of natural disturbance regimes. Our results show that the temperate forest biome was historically heterogeneous rather than uniformly dense, which is consistent with the dependency of much of contemporary European biodiversity on open vegetation and light woodland.
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| 15. |
- Persson, Mattias, et al.
(author)
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Structural effects of naturally occurring human blood group B galactosyltransferase mutations adjacent to the DXD motif
- 2007
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In: Journal of Biological Chemistry. - 1083-351X. ; 282:13, s. 9564-9570
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Journal article (peer-reviewed)abstract
- Human blood group A and B antigens are produced by two closely related glycosyltransferase enzymes. An N-acetylgalactosaminyltransferase (GTA) utilizes UDP-GalNAc to extend H antigen acceptors (Fuc alpha(1-2)Gal beta-OR) producing A antigens, whereas a galactosyltransferase (GTB) utilizes UDP-Gal as a donor to extend H structures producing B antigens. GTA and GTB have a characteristic (DVD213)-D-211 motif that coordinates to a Mn2+ ion shown to be critical in donor binding and catalysis. Three GTB mutants, M214V, M214T, and M214R, with alterations adjacent to the (211)DVD213 motif have been identified in blood banking laboratories. From serological phenotyping, individuals with the M214R mutation show the B,1 variant expressing very low levels of B antigens, whereas those with M214T and M214V mutations give rise to A(weak)B phenotypes. Kinetic analysis of recombinant mutant GTB enzymes revealed that M214R has a 1200-fold decrease in k(cat) compared with wild type GTB. The crystal structure of M214R showed that DVD motif coordination to Mn2+ was disrupted by Arg-214 causing displacement of the metal by a water molecule. Kinetic characterizations of the M214T and M214V mutants revealed they both had GTA and GTB activity consistent with the serology. The crystal structure of the M214T mutant showed no change in DVD coordination to Mn2+. Instead a critical residue, Met-266, which is responsible for determining donor specificity, had adopted alternate conformations. The conformation with the highest occupancy opens up the active site to accommodate the larger A-specific donor, UDP-GalNAc, accounting for the dual specificity.
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| 16. |
- Rosadas, C., et al.
(author)
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Neurofilament Light in CSF and Plasma Is a Marker of Neuronal Damage in HTLV-1-Associated Myelopathy and Correlates With Neuroinflammation
- 2021
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In: Neurology-Neuroimmunology & Neuroinflammation. - : Ovid Technologies (Wolters Kluwer Health). - 2332-7812. ; 8:6
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Journal article (peer-reviewed)abstract
- Background and Objectives To evaluate the usefulness of CSF and plasma neurofilament light (Nf-L) as a biomarker for human T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy (HAM). Methods Nf-L, CXCL10, and neopterin were measured by ELISA in 83 CSF samples obtained from 49 individuals living with HTLV-1/2. Plasma Nf-L was also measured by single molecule array. Results were correlated with duration of disease, age, mobility, CSF cell counts, CSF protein, and HTLV-1 proviral load. Results Nf-L was detected in all CSF samples (median [range] = 575 [791.8-2,349] pg/mL) and positively correlated with markers of inflammation (CXCL10 (r = 0.733), neopterin (r = 0.499), cell count (r = 0.403), and protein levels (r = 0.693) in CSF; p < 0.0015). There was an inverse correlation between Nf-L and duration of disease (r = -0.584, p < 0.0001). Wheelchair-dependent patients had high concentrations of markers of inflammation and neuronal damage. Concentrations of CXCL10, neopterin, and Nf-L remained elevated in follow-up samples (mean follow-up 5.2 years). Nf-L in plasma correlated with concentration of Nf-L, neopterin, CXCL10, and protein in CSF. Conclusions Nf-L in plasma and CSF has potential to be used as a biomarker of disease activity in HAM. Neuronal damage seems to be more intense early in disease but persists long term. Wheelchair-dependent patients have ongoing neuroinflammation.
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| 17. |
- Troll, Valentin, et al.
(author)
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Magmatic differentiation processes at Merapi Volcano : inclusion petrology and oxygen isotopes
- 2013
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In: Journal of Volcanology and Geothermal Research. - : Elsevier. - 0377-0273 .- 1872-6097. ; 261:SI, s. 38-49
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Journal article (peer-reviewed)abstract
- Indonesian volcano Merapi is one of the most hazardous volcanoes on the planet and is characterised by periods of active dome growth and intermittent explosive events. Merapi currently degasses continuously through high temperature fumaroles and erupts basaltic-andesite dome lavas and associated block-and-ash-flows that carry a large range of magmatic, coarsely crystalline plutonic, and meta-sedimentary inclusions. These inclusions are useful in order to evaluate magmatic processes that act within Merapi's plumbing system, and to help an assessment of which phenomena could trigger explosive eruptions. With the aid of petrological, textural, and oxygen isotope analysis we record a range of processes during crustal magma storage and transport, including mafic recharge, magma mixing, crystal fractionation, and country rock assimilation. Notably, abundant calc-silicate inclusions (true xenoliths) and elevated δ18O values in feldspar phenocrysts from 1994, 1998, 2006, and 2010 Merapi lavas suggest addition of limestone and calc-silicate materials to the Merapi magmas. Together with high δ13C values in fumarole gas, crustal additions to mantle and slab-derived magma and volatile sources are likely a steady state process at Merapi. This late crustal input could well represent an eruption trigger due to sudden over-pressurisation of the shallowest parts of the magma storage system independently of magmatic recharge and crystal fractionation. Limited seismic precursors may be associated with this type of eruption trigger, offering a potential explanation for the sometimes erratic behaviour of Merapi during volcanic crises.
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