| 1. |
-
- 2019
-
Tidskriftsartikel (refereegranskat)
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Brownlie, Will, et al.
(författare)
-
Phosphorus reserves, resources and uses
- 2022. - 1
-
Ingår i: Our Phosphorus Future. - : UK Centre for Ecology & Hydrology. ; , s. 20-71
-
Bokkapitel (refereegranskat)abstract
- Five countries hold 85% of the planet’s phosphate rock reserves. High dependency on imported phosphate rock and/or mineral phosphorus fertiliser can contribute to national food system vulnerability. Geological depletion of phosphate rock is not an immediate threat, however geopolitical, institutional, economic, and managerial factors may impact phosphorus access. Improving the efficient use of phosphorus in agriculture and shifting reliance away from mined phosphorus sources by increasing phosphorus recycling may offer the greatest protection against potential phosphorus supply risks.
|
|
| 5. |
- Alisjahbana, A, et al.
(författare)
-
CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung
- 2021
-
Ingår i: Frontiers in immunology. - : Frontiers Media SA. - 1664-3224. ; 12, s. 752104-
-
Tidskriftsartikel (refereegranskat)abstract
- Innate lymphoid cells (ILCs) contribute to immune defense, yet it is poorly understood how ILCs develop and are strategically positioned in the lung. This applies especially to human ILCs due to the difficulty of studying them in vivo. Here we investigated the ontogeny and migration of human ILCs in vivo with a humanized mouse model (“MISTRG”) expressing human cytokines. In addition to known tissue-resident ILC subsets, we discovered CD5-expressing ILCs that predominantly resided within the lung vasculature and in the circulation. CD5+ ILCs contained IFNγ-producing mature ILC1s as well as immature ILCs that produced ILC effector cytokines under polarizing conditions in vitro. CD5+ ILCs had a distinct ontogeny compared to conventional CD5- ILCs because they first appeared in the thymus, spleen and liver rather than in the bone marrow after transplantation of MISTRG mice with human CD34+ hematopoietic stem and progenitor cells. Due to their strategic location, human CD5+ ILCs could serve as blood-borne sentinels, ready to be recruited into the lung to respond to environmental challenges. This work emphasizes the uniqueness of human CD5+ ILCs in terms of their anatomical localization and developmental origin compared to well-studied CD5- ILCs.
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
- Brownlie, Rebecca J, et al.
(författare)
-
Distinct cell-specific control of autoimmunity and infection by FcgammaRIIb.
- 2008
-
Ingår i: The Journal of experimental medicine. - : Rockefeller University Press. - 1540-9538 .- 0022-1007. ; 205:4, s. 883-95
-
Tidskriftsartikel (refereegranskat)abstract
- FcgammaRIIb is an inhibitory Fc receptor expressed on B cells and myeloid cells. It is important in controlling responses to infection, and reduced expression or function predisposes to autoimmunity. To determine if increased expression of FcgammaRIIb can modulate these processes, we created transgenic mice overexpressing FcgammaRIIb on B cells or macrophages. Overexpression of FcgammaRIIb on B cells reduced the immunoglobulin G component of T-dependent immune responses, led to early resolution of collagen-induced arthritis (CIA), and reduced spontaneous systemic lupus erythematosus (SLE). In contrast, overexpression on macrophages had no effect on immune responses, CIA, or SLE but increased mortality after Streptococcus pneumoniae infection. These results help define the role of FcgammaRIIb in immune responses, demonstrate the contrasting roles played by FcgammaRIIb on B cells and macrophages in the control of infection and autoimmunity, and emphasize the therapeutic potential for modulation of FcgammaRIIb expression on B cells in inflammatory and autoimmune disease.
|
|
| 9. |
|
|
| 10. |
|
|
| 11. |
- Brownlie, D, et al.
(författare)
-
Comparison of Lung-Homing Receptor Expression and Activation Profiles on NK Cell and T Cell Subsets in COVID-19 and Influenza
- 2022
-
Ingår i: Frontiers in immunology. - : Frontiers Media SA. - 1664-3224. ; 13, s. 834862-
-
Tidskriftsartikel (refereegranskat)abstract
- Respiratory viral infections with SARS-CoV-2 and influenza viruses commonly induce a strong infiltration of immune cells into the human lung, with potential detrimental effects on the integrity of the lung tissue. Despite comprising the largest fractions of circulating lymphocytes in the lung, rather little is known about how peripheral blood natural killer (NK) cell and T cell subsets are equipped for lung-homing in COVID-19 and influenza. Here, we provide a detailed comparative analysis of NK cells and T cells in patients infected with SARS-CoV-2 or influenza virus, focusing on the protein and gene expression of chemokine receptors known to be involved in recruitment to the lung. For this, we used 28-colour flow cytometry as well as re-analysis of a publicly available single-cell RNA-seq dataset from bronchoalveolar lavage (BAL) fluid. Frequencies of NK cells and T cells expressing CXCR3, CXCR6, and CCR5 were altered in peripheral blood of COVID-19 and influenza patients, in line with increased transcript expression of CXCR3, CXCR6, and CCR5 and their respective ligands in BAL fluid. NK cells and T cells expressing lung-homing receptors displayed stronger phenotypic signs of activation compared to cells lacking lung-homing receptors, and activation was overall stronger in influenza compared to COVID-19. Together, our results indicate a role for CXCR3+, CXCR6+, and/or CCR5+ NK cells and T cells that potentially migrate to the lungs in moderate COVID-19 and influenza patients, identifying common targets for future therapeutic interventions in respiratory viral infections.
|
|
| 12. |
- Brownlie, Rebecca J., et al.
(författare)
-
Lack of the Phosphatase PTPN22 Increases Adhesion of Murine Regulatory T Cells to Improve Their Immunosuppressive Function
- 2012
-
Ingår i: Science Signaling. - : American Association for the Advancement of Science (AAAS). - 1937-9145 .- 1945-0877. ; 5:252, s. 87-87
-
Tidskriftsartikel (refereegranskat)abstract
- The cytoplasmic phosphatase PTPN22 (protein tyrosine phosphatase nonreceptor type 22) plays a key role in regulating lymphocyte homeostasis, which ensures that the total number of lymphocytes in the periphery remains relatively constant. Mutations in PTPN22 confer an increased risk of developing autoimmune diseases; however, the precise function of PTPN22 and how mutations contribute to auto-immunity remain controversial. Loss-of-function mutations in PTPN22 are associated with increased numbers of effector T cells and autoreactive B cells in humans and mice; however, the complete absence of PTPN22 in mice does not result in spontaneous autoimmunity. We found that PTPN22 was a key regulator of regulatory T cell (T-reg) function that fine-tuned the signaling of the T cell receptor and integrins. PTPN22(-/-) T-regs were more effective at immunosuppression than were wild-type T-regs, and they suppressed the activity of PTPN22(-/-) effector T cells, preventing autoimmunity. Compared to wildtype T-regs, PTPN22(-/-) T-regs produced increased amounts of the immunosuppressive cytokine interleukin-10 and had enhanced adhesive properties mediated by the integrin lymphocyte function-associated antigen-1, processes that are critical for T-reg function. This previously undiscovered role of PTPN22 in regulating integrin signaling and T-reg function suggests that PTPN22 may be a useful therapeutic target for manipulating T-reg function in human disease.
|
|
| 13. |
- Brownlie, Will J., et al.
(författare)
-
Opportunities to recycle phosphorus-rich organic materials
- 2022. - 1
-
Ingår i: Our Phosphorus Future. - Edinburgh : UK Centre for Ecology & Hydrology. ; , s. 219-270
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Recycling phosphorus-rich organic wastes and manures is critical for phosphorus sustainability and a transition to a more circular economy for phosphorus. Beyond agronomic benefits, the win-wins are numerous, with benefits to society, environment, economy, and business growth. However, to significantly increase phosphorus recycling, education, awareness-raising, investment in technology and infrastructure, and policy support are urgently needed.
|
|
| 14. |
|
|
| 15. |
|
|
