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- Hammond, Maria, et al.
(author)
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Sensitive detection of aggregated prion protein via proximity ligation
- 2014
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In: Prion. - : Informa UK Limited. - 1933-6896 .- 1933-690X. ; 8:3, s. 261-265
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Journal article (peer-reviewed)abstract
- The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of three or more copies of the specific protein. We have developed a SP-PLA that can detect PrP aggregates in brain homogenates from infected hamsters even after a 10(7)-fold dilution. In contrast, brain homogenate from uninfected animals did not generate a detectable signal at hundred-fold higher concentration. Using either of the two monoclonal anti-PrP antibodies 3F4 and 6H4 we successfully detected low concentrations of aggregated PrP. The presented results provide a proof of concept that this method might be an interesting tool in the development of diagnostic approaches of prion diseases.
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