| 1. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Cheng, Yuxin
(author)
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Optical Interconnects for Next Generation Data Centers : Architecture Design and Resource Allocation
- 2019
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Doctoral thesis (other academic/artistic)abstract
- The current data center architectures based on blade servers and elec- tronic packet switches face several problems, e.g., limited resource utilization, high power consumption and cost, when handling the rapidly growing of data traffic. Optical networks offering ultra-high capacity and requiring low energy consumption are considered as a good option to address these problems. This thesis presents new data center architectures based on optical interconnects and transmissions, and evaluates performance by extensive simulations.The first main contribution of the thesis is to introduce a passive optical top-of-rack interconnect (POTORI) architecture. The data plane of POTORI mainly consists of passive components to interconnect the servers within the rack. Using the passive components makes it possible to significantly reduce power consumption while achieving high reliability in a cost-efficient way. In addition, the POTORI’s control plane is based on a centralized controller, which is responsible for coordinating the communications among the servers in the rack. A cycle-based medium access control (MAC) protocol and a dy- namic bandwidth allocation (DBA) algorithm are designed for the POTORI to efficiently manage the exchange of control messages and the data transmis- sion inside the rack. Simulation results show that under realistic DC traffic scenarios, the POTORI with the proposed DBA algorithm is able to achieve an average packet delay below 10 μs with the use of fast tunable optical transceivers.The second main contribution of the thesis is to investigate rack-scale disaggregated data center (DDC) architecture for improving resource utiliza- tion. In contrast to the traditional DC with blade servers that integrate various types of resources (e.g., central processing unit (CPU), memory) in a chassis, the rack-scale DDC contains fully decoupled resources held on differ- ent blades, referred to as resource blades. The resource blades are required to be interconnected within the rack by an ultra-high bandwidth optical in- terconnect through the optical interfaces (OIs). A resource allocation (RA) algorithm is proposed to efficiently schedule the resources in the DDC for virtual machine requests. Results show that with sufficient bandwidth on the OIs, the rack-scale DDC with the proposed RA algorithm can achieve 20% higher resource utilization and make 30% more revenue comparing to the traditional DC.
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| 3. |
- Fong, Wen-fai, et al.
(author)
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Short GRB Host Galaxies. I. Photometric and Spectroscopic Catalogs, Host Associations, and Galactocentric Offsets
- 2022
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In: Astrophysical Journal. - : American Astronomical Society. - 0004-637X .- 1538-4357. ; 940:1
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Journal article (peer-reviewed)abstract
- We present a comprehensive optical and near-infrared census of the fields of 90 short gamma-ray bursts (GRBs) discovered in 2005–2021, constituting all short GRBs for which host galaxy associations are feasible (≈60% of the total Swift short GRB population). We contribute 274 new multi-band imaging observations across 58 distinct GRBs and 26 spectra of their host galaxies. Supplemented by literature and archival survey data, the catalog contains 542 photometric and 42 spectroscopic data sets. The photometric catalog reaches 3σ depths of ≳24–27 mag and ≳23–26 mag for the optical and near-infrared bands, respectively. We identify host galaxies for 84 bursts, in which the most robust associations make up 56% (50/90) of events, while only a small fraction, 6.7%, have inconclusive host associations. Based on new spectroscopy, we determine 18 host spectroscopic redshifts with a range of z ≈ 0.15–1.5 and find that ≈23%–41% of Swift short GRBs originate from z > 1. We also present the galactocentric offset catalog for 84 short GRBs. Taking into account the large range of individual measurement uncertainties, we find a median of projected offset of ≈7.7 kpc, for which the bursts with the most robust associations have a smaller median of ≈4.8 kpc. Our catalog captures more high-redshift and low-luminosity hosts, and more highly offset bursts than previously found, thereby diversifying the population of known short GRB hosts and properties. In terms of locations and host luminosities, the populations of short GRBs with and without detectable extended emission are statistically indistinguishable. This suggests that they arise from the same progenitors, or from multiple progenitors, which form and evolve in similar environments. All of the data products are available on the Broadband Repository for Investigating Gamma-Ray Burst Host Traits website.
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| 4. |
- Lin, Yuanbao, et al.
(author)
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One-Step Blade-Coated Highly Efficient Nonfullerene Organic Solar Cells with a Self-Assembled Interfacial Layer Enabled by Solvent Vapor Annealing
- 2019
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In: Solar RRL. - : Wiley. - 2367-198X. ; 3:8
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Journal article (peer-reviewed)abstract
- A pronounced enhancement of the power conversion efficiency (PCE) by 38% is achieved in one-step doctor-blade printing organic solar cells (OSCs) via a simple solvent vapor annealing (SVA) step. The organic blend composed of a donor polymer, a nonfullerene acceptor, and an interfacial layer (IL) molecular component is found to phase-separate vertically when exposed to a solvent vapor-saturated atmosphere. Remarkably, the spontaneous formation of a fine, self-organized IL between the bulk heterojunction (BHJ) layer and the indium tin oxide (ITO) electrode facilitated by SVA yields solar cells with a significantly higher PCE (11.14%) than in control devices (8.05%) without SVA and in devices (10.06%) made with the more complex two-step doctor-blade printing method. The stratified nature of the ITO/IL/BHJ/cathode is corroborated by a range of complementary characterization techniques including surface energy, cross-sectional scanning electron microscopy, grazing incidence wide angle X-ray scattering, and X-ray photoelectron spectroscopy. This study demonstrates that a spontaneously formed IL with SVA treatment combines simplicity and precision with high device performance, thus making it attractive for large-area manufacturing of next-generation OSCs.
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| 5. |
- Zhang, Zhi, et al.
(author)
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Customized Structural Color Filters by Pixel-Level Electrothermal Regulation
- 2023
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In: Laser & Photonics reviews. - : Wiley. - 1863-8880 .- 1863-8899. ; 17:5
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Journal article (peer-reviewed)abstract
- Reflective structural colors have backlight-free supremacy and can provide energy-efficient and environmental-friendly color production for many applications. However, they are hindered by the complex process and incorrect colors after fabrication. Here, a pixelated electrothermal oxidation technique is proposed that can precisely tailor and produce reflective structural colors covering the entire visible range, providing the possibility for customized structural color filter arrays from a titanium canvas. Furthermore, as a demonstration, a photonic-firework-like color filter array is achieved in a single step via thermal engineering. Based on this one-step-forming color array, a computational spectrometer is also realized featuring a resolvability of 10 nm and operating bandwidth covering the whole visible range. Considering its compactness and mass production capability, this method has numerous potential applications in, e.g., imaging, anti-counterfeiting, printing, color display, and spectroscopy.
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