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1.	Enkirch, T, et al. (author) Improving preparedness to respond to cross-border hepatitis A outbreaks in the European Union/European Economic Area: towards comparable sequencing of hepatitis A virus 2019 In: Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin. - 1560-7917. ; 24:28, s. 13-23 Journal article (peer-reviewed)
2.	Persson, Sofia, et al. (author) A new assay for quantitative detection of hepatitis A virus 2021 In: Journal of Virological Methods. - : Elsevier BV. - 0166-0934 .- 1879-0984. ; 288 Journal article (peer-reviewed)abstract Hepatitis A virus (HAV) is mainly transmitted via contaminated food or water or through person-to-person contact. Here, we describe development and evaluation of a reverse transcription droplet digital PCR (RTddPCR) and reverse transcription real-time PCR (RT-qPCR) assay for detection of HAV in food and clinical specimens. The assay was evaluated by assessing limit of detection, precision, matrix effects, sensitivity and quantitative agreement. The 95 % limit of detection (LOD95 %) was 10 % higher for RT-ddPCR than for RTqPCR. A Bayesian model was used to estimate precision on different target concentrations. From this, we found that RT-ddPCR had somewhat greater precision than RT-qPCR within runs and markedly greater precision between runs. By analysing serum from naturally infected persons and a naturally contaminated food sample, we found that the two methods agreed well in quantification and had comparable sensitivities. Tests with artificially contaminated food samples revealed that neither RT-ddPCR nor RT-qPCR was severely inhibited by presence of oysters, raspberries, blueberries or leafy-green vegetables. For this assay, we conclude that RT-qPCR should be considered if rapid, qualitative detection is the main interest and that RT-ddPCR should be considered if precise quantification is the main interest. The high precision of RT-ddPCR allows for detection of small changes in viral concentration over time, which has direct implications for both food control and clinical studies.

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Type of publication: journal article (2)

Type of content: peer-reviewed (2)

Author/Editor: Enkirch, T (2); Poljak, M (1); Ellström, Patrik (1); Eriksson, R (1); Norder, Helene (1); Alm, E (1); show more...; Thornton, L (1); Persson, Sofia (1); Epstein, J (1); Christova, I (1); Kontio, M (1); Faber, M (1); Mellou, K (1); Borg, ML (1); Vold, L (1); Severi, E (1); Dean, J (1); Fischer, TK (1); Simonsson, M (1); Love, A (1); Nemecek, V. (1); Vennema, H. (1); Ciccaglione, AR (1); Bruni, R (1); Ngui, SL (1); Balogun, K (1); Takacs, M (1); Hettmann, A (1); Korotinska, R (1); Avellon, A (1); Munoz-Chimeno, M (1); de Sousa, R (1); Janta, D (1); Klamer, S (1); Suin, V (1); Aberle, SW (1); Holzmann, H (1); Ederth, JL (1); Sundqvist, L (1); Roque-Afonso, AM (1); Filipovic, SK (1); Stene-Johansen, K (1); Midgley, S (1); Wenzel, JJ (1); Takkinen, J (1); Leitmeyer, K (1); Karlsson, Måns (1); show less...

University: University of Gothenburg (1); Uppsala University (1); Stockholm University (1); Karolinska Institutet (1)

Language: English (2)

Research subject (UKÄ/SCB): Natural sciences (1); Medical and Health Sciences (1)

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