| 1. |
- Alexandersson, Erik, et al.
(author)
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Whole gene family expression and drought stress regulation of aquaporins
- 2005
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In: Plant Molecular Biology. - : Springer Science and Business Media LLC. - 1573-5028 .- 0167-4412. ; 59:3, s. 469-484
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Journal article (peer-reviewed)abstract
- Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level.
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| 2. |
- Anhede, Jesper, et al.
(author)
-
Film - Min plats i biosfären! Om forskning i Unesco Biosfärområde Vänerskärgården med Kinnekulle : Film - My Place in the Biosphere - Results from research in a Swedish Bioshere Reserve
- 2020
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In: Film producerad av Biosfärområde Vänerskärgården med Kinnekulle i samarbete med Göteborgs universitet, Högskolan i Gävle och Vänermuseet.
-
Other publication (other academic/artistic)abstract
- Min plats i biosfären är en skrift och en vandringsutställning som visar forskningsresultat om vilken roll kulturmiljön och de kulturella ekosystemtjänsterna kulturarv och platsidentitet har för människors välbefinnande och för hållbar landskapsförvaltning inom Unesco Biosfärområde Vänerskärgården med Kinnekulle. Innehållet baseras på forskningsprojektet Kulturmiljö och kulturarv som en del av hållbar landskapsförvaltning utfört av forskare vid Göteborgs universitet och Högskolan i Gävle. Utställningen visas på de här platserna i biosfärområdet år 2020: * Juni - Stenhusbacken, Lidköping * Juli - Bäcken, Ekudden, Mariestad * Augusti - Kalkstensbrottet, Götene. Ladda ner skriften här: https://gupea.ub.gu.se/handle/2077/62574 Mer info: https://www.vanerkulle.se #Minplatsibiosfären
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| 3. |
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| 4. |
- Boud, David, et al.
(author)
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Observing interprofessional simulation
- 2019
-
In: Interprofessional Simulation in Health Care. - Cham : Springer. - 9783030195410 - 9783030195427 ; , s. 115-137
-
Book chapter (peer-reviewed)abstract
- This chapter has a particular focus on the observers’ role in simulation-based learning activities. Simulation-based learning is often organised so that participants rotates between active participation in the scenario and participation as observers. The research examples provided show that the conditions for learning are related to the locations where and the ways the observers are situated, and to how the instructions to the observers are formulated. Arguments are put forward that the observers’ role in simulation has unexploited potential for developing skills of noticing.
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| 5. |
- Gustafsson, Sofia, et al.
(author)
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Blood-Brain Barrier Integrity in a Mouse Model of Alzheimer’s Disease With or Without Acute 3D6 Immunotherapy
- 2018
-
In: Neuropharmacology. - : Elsevier BV. - 0028-3908 .- 1873-7064. ; 143, s. 1-9
-
Journal article (peer-reviewed)abstract
- The blood-brain barrier (BBB) is suggested to be compromised in Alzheimer's disease (AD). The concomitant presence of vascular amyloid beta (AD) pathology, so called cerebral amyloid angiopathy (CAA), also predisposes impairment of vessel integrity. Additionally, immunotherapy against A beta may lead to further damage of the BBB. To what extent this affects the BBB passage of molecules is debated. The current study aimed to investigate BBB integrity to large molecules in transgenic mice displaying abundant A beta pathology and age matched wild type animals, with or without acute anti-A beta antibody treatment. Animals were administered a single i.v. injection of PBS or 3D6 (10 mg/kg), i.e. the murine version of the clinically investigated A beta antibody bapineuzumab, supplemented with [(125)]3D6. Three days post injections, a 4 kDa FITC and a 150 kDa Antonia Red dextran were administered i.v. to all animals. After termination, fluorescent detection in brain and serum was used for the calculation of dextran brain-to-blood concentration ratios. Further characterization of antibody fate and the presence of CAA were investigated using radioactivity measurements and Congo red staining. BBB passage of large molecules was equally low in wild type and transgenic mice, suggesting an intact BBB despite A beta pathology. Neither was the BBB integrity affected by acute 3D6 treatment. However, CAA was confirmed in the transgenes and local antibody accumulations were observed in the brain, indicating CAA-antibody interactions. The current study shows that independently of A beta pathology or acute 3D6 treatment, the BBB is intact, without extensive permeability to large molecules, including the 3D6 antibody.
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| 6. |
- Gustavsson, Sofia, et al.
(author)
-
A novel plant major intrinsic protein in Physcomitrella patens most similar to bacterial glycerol channels
- 2005
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In: Plant Physiology. - : Oxford University Press (OUP). - 1532-2548 .- 0032-0889. ; 139:1, s. 287-295
-
Journal article (peer-reviewed)abstract
- A gene encoding a novel fifth type of major intrinsic protein (MIP) in plants has been identified in the moss Physcomitrella patens. Phylogenetic analyses show that this protein, GlpF-like intrinsic protein (GIP1;1), is closely related to a subclass of glycerol transporters in bacteria that in addition to glycerol are highly permeable to water. A likely explanation of the occurrence of this bacterial-like MIP in P. patens is horizontal gene transfer. The expressed P. patens GIP1;1 gene contains five introns and encodes a unique C-loop extension of approximately 110 amino acid residues that has no obvious similarity with any other known protein. Based on alignments and structural comparisons with other MIPs, GIP1;1 is suggested to have retained the permeability for glycerol but not for water. Studies on heterologously expressed GIP1;1 in Xenopus laevis oocytes confirm the predicted substrate specificity. Interestingly, proteins of one of the plant-specific subgroups of MIPs, the NOD26-like intrinsic proteins, are also facilitating the transport of glycerol and have previously been suggested to have evolved from a horizontally transferred bacterial gene. Further studies on localization and searches for GIP1;1 homologs in other plants will clarify the function and significance of this new plant MIP.
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| 7. |
- Gustavsson, Sofia
(author)
-
Plant major intrinsic proteins. Identification and characterization of novel water channel-like protiens.
- 2005
-
Doctoral thesis (other academic/artistic)abstract
- The Major Intrinsic Proteins, MIPs, constitute a large, ancient superfamily of membrane proteins that are found in all kingdoms of life. Most MIPs facilitate passive diffusion of water across biological membranes (aquaporins), although many channels are also permeable for glycerol and other small uncharged solutes, (aquaglyceroporins). Other substrates reported for specific MIPs are anions, carbon dioxide, arsenic and boric acid. MIPs are predominantly important in processes involving water regulation and osmoregulation, but have also been suggested to be involved in carbohydrate metabolism. In the genome of the model plant Arabidopsis thaliana, 35 MIP-encoding genes were identified. By sequence homology and phylogenetic analyses, these genes were divided into four subfamilies, Plasma membrane Intrinsic Proteins, PIPs, Tonoplast Intrinsic Proteins, TIPs, Nod26-like Intrinsic Proteins, NIPs, and Small basic Intrinsic proteins, SIPs. Based on the phylogenetic analyses and the already commonly used names, a new nomenclature, which now is widely accepted, was proposed. The SIPs form the most recently identified subfamily, and this divergent family is also the poorest characterized. The constriction region of SIPs is unlike filters of both aguaglyceroporins and aquaporins, suggesting different substrates than glycerol or water. Expression studies by promoter::GUS fusions imply that SIP1;1 is expressed in roots, trichomes, stigma, style and stamen. Vascular root localization of SIP1;1 was also observed on the protein level by immunogold labeling, which also labeled guard cells in leaves. SIP2;1 was also localized in guard cells in addition to roots, predominantly in the center of the vasculature. The subcellular localization was investigated by aqueous two-phase partitioning and immunoblotting. Both SIP1;1 and SIP2;1 appear in the plasma membrane fraction when isolated from leaves, but mainly in the fraction containing internal membranes when isolated from roots. Water and glycerol permeability for SIP1;1 and SIP2;1 was investigated by heterologous expression in Xenopus laevis oocytes, but no permeability for either molecule could be observed. In the moss Physcomitrella patens a MIP more similar to bacterial aquaglyceroporins than to any other known plant MIP was identified. Most likely, this protein, GIP1;1, was recruited by plants from bacteria by horizontal gene transfer. GIP1;1 (GIP for GlpF-like Intrinsic Protein) was predicted to be a glycerol selective channel, which subsequently was confirmed after heterologous expression in Xenopus oocytes.
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| 8. |
- Holmgren, Sofia, et al.
(author)
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Cytokine mRNA expression in bronchoalveolar lavage cells during Dictyocaulus viviparus infection in calves
- 2014
-
In: Parasite Immunology. - : Wiley. - 0141-9838 .- 1365-3024. ; 36, s. 78-86
-
Journal article (peer-reviewed)abstract
- The purpose of this study was to monitor local cytokine responses to Dictyocaulus viviparus in calves during primary infection and re-infection. Bronchoalveolar lavage fluid (BALF) was collected weekly from experimentally infected calves and interleukin-2 (IL-2), IL-4, IL-5, IL-10 and IFN- mRNA expression was quantified in BALF cells. The major finding was a prominent transient increase in IL-4 mRNA expression, compared with that of uninfected calves, observed in BALF cells collected 2-3weeks post-primary D.viviparus infection. At 2weeks post-infection, macroscopic worms were also first observed in BALF. Calves re-infected after 10weeks were partially immune which was evident at slaughter 5weeks post-infection as a lower worm burden than in previously naive calves infected at the same time. IL-4 mRNA expression in BALF cells 2weeks post-re-infection was increased compared with that of uninfected animals but not as high as that of primarily infected calves. BALF cell expression of the other cytokines tested for was not as clearly effected by the D.viviparus infection. It seems likely that the strong IL-4 response observed during primary infection reflects an innate response to the worms that may initiate an ensuing Th2 response, which confers protective immunity.
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| 9. |
- Johanson, Urban, et al.
(author)
-
A new subfamily of major intrinsic proteins in plants.
- 2002
-
In: Molecular biology and evolution. - 0737-4038. ; 19:4, s. 456-461
-
Journal article (peer-reviewed)abstract
- The major intrinsic proteins (MIPs) form a large protein family of ancient origin and are found in bacteria, fungi, animals, and plants. MIPs act as channels in membranes to facilitate passive transport across the membrane. Some MIPs allow small polar molecules like glycerol or urea to pass through the membrane. However, the majority of MIPs are thought to be aquaporins (AQPs), i.e., they are specific for water transport. Plant MIPs can be subdivided into the plasma membrane intrinsic protein, tonoplast intrinsic protein, and NOD26-like intrinsic protein subfamilies. By database mining and phylogenetic analyses, we have identified a new subfamily in plants, the Small basic Intrinsic Proteins (SIPs). Comparisons of sequences from the new subfamily with conserved amino acid residues in other MIPs reveal characteristic features of SIPs. Possible functional consequences of these features are discussed in relation to the recently solved structures of AQP1 and GlpF. We suggest that substitutions at conserved and structurally important positions imply a different substrate specificity for the new subfamily.
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| 10. |
- Johanson, Urban, et al.
(author)
-
The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants
- 2001
-
In: Plant Physiology. - : Oxford University Press (OUP). - 1532-2548 .- 0032-0889. ; 126:4, s. 1358-1369
-
Journal article (peer-reviewed)abstract
- Major intrinsic proteins (MIPs) facilitate the passive transport of small polar molecules across membranes. MIPs constitute a very old family of proteins and different forms have been found in all kinds of living organisms, including bacteria, fungi, animals, and plants. In the genomic sequence of Arabidopsis, we have identified 35 different MIP-encoding genes. Based on sequence similarity, these 35 proteins are divided into four different subfamilies: plasma membrane intrinsic proteins, tonoplast intrinsic proteins, NOD26-like intrinsic proteins also called NOD26-like MIPs, and the recently discovered small basic intrinsic proteins. In Arabidopsis, there are 13 plasma membrane intrinsic proteins, 10 tonoplast intrinsic proteins, nine NOD26-like intrinsic proteins, and three small basic intrinsic proteins. The gene structure in general is conserved within each subfamily, although there is a tendency to lose introns. Based on phylogenetic comparisons of maize (Zea mays) and Arabidopsis MIPs (AtMIPs), it is argued that the general intron patterns in the subfamilies were formed before the split of monocotyledons and dicotyledons. Although the gene structure is unique for each subfamily, there is a common pattern in how transmembrane helices are encoded on the exons in three of the subfamilies. The nomenclature for plant MIPs varies widely between different species but also between subfamilies in the same species. Based on the phylogeny of all AtMIPs, a new and more consistent nomenclature is proposed. The complete set of AtMIPs, together with the new nomenclature, will facilitate the isolation, classification, and labeling of plant MIPs from other species.
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| 11. |
- Karrbom Gustavsson, Tina, 1973-, et al.
(author)
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Procurement Research: Current State and Future Challenges in the Nordic Countries
- 2019
-
In: 10th Nordic Conference on Construction Economics and Organization (Emerald Reach Proceedings Series, Volume 2). - : Emerald Group Publishing Limited. ; , s. 195-204, s. 195-204
-
Conference paper (peer-reviewed)abstract
- PurposeThe purpose of the study is to map previous and current construction procurement research to further develop the research in the Nordic counties.Design/Methodology/ApproachMapping of previous and current research based on search in national database. The analysis is based on research perspectives, empirical contexts and research methods.FindingsThat the blind spots are partly overlapping, but that there is potential for knowledge transfer in some areas. There is also the potential for a Nordic research program on one or several of the blind spots.Research Limitations/ImplicationsThe study is limited to PhD and licentiate-thesis reports in Norway and Sweden. Further research should include the other Nordic countries and a more extensive literature review including journal articles to broaden the scope. Findings have implications on collaborative Nordic research initiatives, knowledge transfer and in a longer perspective on the level of procurement knowledge in industry and society.Practical ImplicationsFindings provide a base for future research collaborations, initiatives and applications.Originality/ValueFindings provide a comprehensive understanding of construction procurement research in the Nordic countries, starting with Norway and Sweden. This understanding is needed for developing research collaborations and applications.
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| 12. |
- Lindstrand, Anna, et al.
(author)
-
From cytogenetics to cytogenomics : whole-genome sequencing as a first-line test comprehensively captures the diverse spectrum of disease-causing genetic variation underlying intellectual disability
- 2019
-
In: Genome Medicine. - : BMC. - 1756-994X. ; 11:1
-
Journal article (peer-reviewed)abstract
- BackgroundSince different types of genetic variants, from single nucleotide variants (SNVs) to large chromosomal rearrangements, underlie intellectual disability, we evaluated the use of whole-genome sequencing (WGS) rather than chromosomal microarray analysis (CMA) as a first-line genetic diagnostic test.MethodsWe analyzed three cohorts with short-read WGS: (i) a retrospective cohort with validated copy number variants (CNVs) (cohort 1, n=68), (ii) individuals referred for monogenic multi-gene panels (cohort 2, n=156), and (iii) 100 prospective, consecutive cases referred to our center for CMA (cohort 3). Bioinformatic tools developed include FindSV, SVDB, Rhocall, Rhoviz, and vcf2cytosure.ResultsFirst, we validated our structural variant (SV)-calling pipeline on cohort 1, consisting of three trisomies and 79 deletions and duplications with a median size of 850kb (min 500bp, max 155Mb). All variants were detected. Second, we utilized the same pipeline in cohort 2 and analyzed with monogenic WGS panels, increasing the diagnostic yield to 8%. Next, cohort 3 was analyzed by both CMA and WGS. The WGS data was processed for large (>10kb) SVs genome-wide and for exonic SVs and SNVs in a panel of 887 genes linked to intellectual disability as well as genes matched to patient-specific Human Phenotype Ontology (HPO) phenotypes. This yielded a total of 25 pathogenic variants (SNVs or SVs), of which 12 were detected by CMA as well. We also applied short tandem repeat (STR) expansion detection and discovered one pathologic expansion in ATXN7. Finally, a case of Prader-Willi syndrome with uniparental disomy (UPD) was validated in the WGS data.Important positional information was obtained in all cohorts. Remarkably, 7% of the analyzed cases harbored complex structural variants, as exemplified by a ring chromosome and two duplications found to be an insertional translocation and part of a cryptic unbalanced translocation, respectively.ConclusionThe overall diagnostic rate of 27% was more than doubled compared to clinical microarray (12%). Using WGS, we detected a wide range of SVs with high accuracy. Since the WGS data also allowed for analysis of SNVs, UPD, and STRs, it represents a powerful comprehensive genetic test in a clinical diagnostic laboratory setting.
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| 13. |
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| 14. |
- Maurice, Christian, et al.
(author)
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Stabilization of CCA-contaminated soil with iron products : a field experiment
- 2005
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In: Kalmar ECO-TECH '05. - : Högskolan i Kalmar.
-
Conference paper (peer-reviewed)abstract
- Chemical stabilization of metals is lately considered as a possible pretreatment for soil contaminated with average levels of trace elements. The element mobility in soil can be altered by adding soil amendments that can adsorb, complex, or co-precipitate trace elements. As a consequence, pollutant spreading from the contaminated soil and effect on the recipient can be reduced. The different contaminants originating from wood impregnation chemicals, e.g. Cu, Cr, and As limit the choice of amendments because e.g. large pH fluctuations and consequent mobilization of Cu or As should be avoided. The results show that the leaching of arsenic is lowest in the lysimeter with 15% Fe3O4. In both lysimeters with untreated soil and with 1% Fe0, the arsenic leaching seems to decrease with the sampling depth. The leaching of copper is generally low. Further the addition of iron seems to increase the leaching of manganese and nickel but to reduce the leaching of zinc. Results from the laboratory experiment show that the arsenic content in the leachate is lowest with the highest mixture of magnetite. Mixing is one of the key issues when discussing the treatment efficiency and possible use of the treated soil. The results so far indicate that magnetite can be used for treatment of CCA contaminated soil also at a large scale. Reduction of both arsenic and copper using a single amendment is challenging as they behave opposite. Magnetite seems to be a promising amendment even though a high amount of amendment needs to be added. Moreover, the potential establishment of reducing conditions at larger depths in the soil is of concern since this might lead to a rapid increase in arsenic leaching.
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| 15. |
- Maurice, Christian, et al.
(author)
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Techniques for the stabilization and assessment of treated copper- chromium- and arsenic-contaminated soil
- 2007
-
In: Ambio. - 0044-7447 .- 1654-7209. ; 36:6, s. 430–6-
-
Journal article (peer-reviewed)abstract
- Remediation mainly based on excavation and burial of the contaminated soil is impractical with regard to the large numbers of sites identified as being in need of remediation. Therefore alternative methods are needed for brownfield remediation. This study was conducted to assess a chemical stabilisation procedure of CCA-contaminated soil using iron-containing blaster sand or oxygen scarfing granulate. The stabilisation technique was assessed with regard to the feasibility of mixing ameliorants at an industrial scale and the efficiency of the stabilisation under different redox conditions. The stability was investigated under natural conditions in 1-m3 lysimeters in a field experiment and the effect of redox conditions was assessed in a laboratory experiment (10 l). The treatments with high additions of ameliorant (8 and 17%) were more successful in both the laboratory and field experiments, even though there was enough iron on a stochiometric basis even at the lowest addition rates (0.1 and 1%). The particle size of the iron and the mixing influenced the stabilisation efficiency. The development of anaerobic conditions, simulated by water saturation, increases the fraction of AsIII and, consequently, arsenic mobility. The use of high concentrations of OSG under aerobic conditions increased the concentrations of Ni and Cu in the pore water. However, under anaerobic conditions, it decreased the arsenic leaching compared to the untreated soil and Ni and Cu leaching was not critical. The final destination of the treated soil should govern the amendment choice, e.g. an OSG concentration around 10% may be suitable if the soil is to be landfilled under anaerobic conditions. Alternatively, the soil mixed with 1% BS could be kept under aerobic conditions in a landfill cover or in situ at brownfield site. In addition, the treatment with BS appeared to produce better effects in the long term than with OSG.
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| 16. |
- Myhrman, Sofia, et al.
(author)
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Unexpected details regarding nosocomial transmission revealed by whole-genome sequencing of SARS-CoV-2
- 2022
-
In: Infection Control and Hospital Epidemiology. - : Cambridge University Press (CUP). - 0899-823X .- 1559-6834. ; 43:10, s. 1403-1407
-
Journal article (peer-reviewed)abstract
- Objective: Effective infection prevention and control (IPC) measures are key for protecting patients from nosocomial infections and require knowledge of transmission mechanisms in different settings. We performed a detailed outbreak analysis of the transmission and outcome of coronavirus disease 2019 (COVID-19) in a geriatric ward by combining whole-genome sequencing (WGS) with epidemiological data. Design: Retrospective cohort study. Setting: Tertiary care hospital. Participants: Patients and healthcare workers (HCWs) from the ward with a nasopharyngeal sample (NPS) positive for SARS-CoV-2 RNA during the outbreak period. Methods: Patient data regarding clinical characteristics, exposure and outcome were collected retrospectively from medical records. Stored NPS from 32 patients and 15 HCWs were selected for WGS and phylogenetic analysis. Results: Median patient age was 84 years and 17/32 (53%) were male. Fourteen patients (44%) died within 30 days after sampling. Viral load was significantly higher among the deceased. WGS was successful in 28/32 (88%) patient samples and 14/15 (93%) HCW samples. Three separate viral clades were identified, whereof one clade and two subclades among both patient and HCW samples. Integrated epidemiological and genetic analysis revealed six probable transmission events between patients and supported hospital-acquired COVID-19 in 25/32 patients. Conclusion: WGS provided a deep insight into the outbreak dynamics and true extent of nosocomial COVID-19. The extensive transmission between patients and HCWs indicated that current IPC measures were insufficient. We suggest increased use of WGS in outbreak investigations for identification of otherwise unknown transmission links and evaluation of IPC measures.
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| 17. |
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| 18. |
- Noren, Håkan, 1948, et al.
(author)
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STAN in clinical practice--the outcome of 2 years of regular use in the city of Gothenburg
- 2006
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In: Am J Obstet Gynecol. ; 195:1, s. 7-15
-
Journal article (peer-reviewed)abstract
- OBJECTIVE: The purpose of this study was to monitor the introduction of the STAN-methodology (Noventa Medical, Moelndal, Sweden). STUDY DESIGN: This was a prospective observational study covering the total population of deliveries at term during 2 years. Four thousand eight hundred and thirty out of 14,687 term pregnancies were monitored using the STAN S 21 fetal heart monitor and the associated clinical guidelines. Cord artery metabolic acidosis, neonatal outcome, and rates of operative deliveries for fetal distress were assessed. RESULTS: The annual rate of STAN usage increased from 28.1% to 37.7% and was associated with a significant reduction in metabolic acidosis rate in the total population from 0.76% to 0.44% (P < .05). The compliance with the clinical guidelines increased in cases requiring intervention. The rates for moderate/severe hypoxic neonatal encephalopathy were consistently low, 0.55 and 0.68 per 1000 deliveries, respectively, and corresponding to previous findings. The rate of operative delivery did not change during the 2 years in the total population. CONCLUSION: Increasing STAN usage provided consistent improvements in fetal outcome equalling those noted in the Swedish randomized controlled trial (RCT) without increasing operative interventions for fetal distress.
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| 19. |
- Ossman, Lisa, et al.
(author)
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Nulägesanalys för satsning på NNE byggnader : slutrapport etapp 1
- 2012
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Reports (other academic/artistic)abstract
- Det övergripande syftet med uppdraget är att få en detaljerad bild över byggnaders energiprestanda i Sverige. Den första etappen, förstudien, går ut på att beskriva metoden för hur denna analys ska göras. Som etapp 2 genomförs själva nulägesanalysen och slut¬ligen i etapp 3 analyseras resultat och slutsatser presenteras. I denna rapport redogörs för etapp 1, dvs metodbeskrivningen för nulägesanalysen. Nulägesanalysen ska senare kunna användas för att följa upp hur satsningar på Näranoll¬energibyggnader (NNE) påverkar energianvändningen i hela byggnadsbeståndet, inte bara i de faktiska demonstrationsprojekt som beviljas stöd och där uppföljning är betydligt enklare. Resultatet av nulägesanalysen, och den uppföljande analysen (planerad till 2015), ska i förlängningen kunna vara ett stöd när de nationella målnivåerna för näranollenergi-byggnader bestäms. I denna rapport redogörs för etapp 1, dvs metodbeskrivningen för nulägesanalysen. Rapporten beskriver vilka statistikkällor som finns idag samt en metod för hur dessa kan bidra till en mer detaljerad bild av byggnaders energiprestanda under ett valt referensår.
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| 20. |
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| 21. |
- Wallman, Andy, et al.
(author)
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Swedish Students and Preceptors Perceptions of What Students Learn in a Six-Month Advanced Pharmacy Practice Experience
- 2011
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In: American Journal of Pharmaceutical Education. - : American Association of Colleges of Pharmacy. - 0002-9459 .- 1553-6467. ; 75:10, s. 1-10
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Journal article (peer-reviewed)abstract
- Objective. To identify what pharmacy students learn during the 6-month advanced pharmacy practice experience (APPE) in Sweden. less thanbrgreater than less thanbrgreater thanMethods. Semi-structured interviews were conducted with 18 pharmacy APPE students and 17 pharmacist preceptors and analyzed in a qualitative directed content analysis using a defined workplace learning typology for categories. less thanbrgreater than less thanbrgreater thanResults. The Swedish APPE provides students with task performance skills for work at pharmacies and social and professional knowledge, such as teamwork, how to learn while in a work setting, self-evaluation, understanding of the pharmacist role, and decision making and problem solving skills. Many of these skills and knowledge are not accounted for in the curricula in Sweden. Using a workplace learning typology to identify learning outcomes, as in this study, could be useful for curricula development. less thanbrgreater than less thanbrgreater thanConclusions. Exploring the learning that takes place during the APPE in a pharmacy revealed a broad range of skills and knowledge that students acquire.
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| 22. |
- Zwedberg, Sofia, 1957-
(author)
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Ville amma! : En hermeneutisk studie av mödrar med amningsbesvär; deras upplevelser, problemhantering samt amningskonsultativa möten
- 2010
-
Doctoral thesis (other academic/artistic)abstract
- The focus is on mothers who asked for help because of initial problems with breastfeeding, i.e., their feelings and experiences, how they cope with their difficulties, and the consultative meetings with a midwife. Taking as a point of departure the ideas that mothers have about breastfeeding, the aim is to describe how mothers experience their situation and themselves as new mothers, when they have problems with breastfeeding, and, to find out how mothers experience the consultative situation as well as their own participation and responsibility. Fourteen mothers, who expressed a wish to breastfeed and who asked for help during their stay in the maternity ward, were video-taped during individual consultations with a midwife. Three months later each mother was interviewed about her experiences and feelings as regards the consultations. The outcomes were analysed according to a hermeneutical approach, from an interactionist perspective and, in addition, using crisis theory as a theoretical basis. This thesis illuminates the interactionist perspective on three different system levels: the norms in society in relation to the mother’s expectations about breastfeeding, the mother’s intra-psychological process and the consultative meeting. The results demonstrate that the women had thought of breastfeeding as a ‘door opener’ into the new role of motherhood. When the mother instead encountered a breastfeeding situation that did not meet with her expectations, there were two things that stood out very clearly; i.e., a feeling of inadequacy, and a constant internal as well as external questioning of herself. The fact that breastfeeding did not turn out as expected could also trigger a crisis reaction. It was important that the mother had a feeling that the midwife understood her, so what she was talking about became meaningful. This in turn could lead to a shift in attitude so that the mother changed from a closed position to an open and, thus, could begin to look forward. If breastfeeding did not turn out the way the mother had hoped her self-image was influenced and ‘coming into existence” as a mother was more difficult. To be involved and responsible had different meanings depending on where the mother was in the process, which meant that the mothers wanted different kinds of support on the way. One condition for making this possible was the consultative meeting in a manner of reciprocity.
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