1. |
- Hagell, Peter, et al.
(author)
-
Apomorphine formulation may influence subcutaneous complications from continuous subcutaneous apomorphine infusion in Parkinson's disease
- 2020
-
In: Journal of Neurology. - 0340-5354 .- 1432-1459. ; 267:11, s. 3411-3417
-
Journal article (peer-reviewed)abstract
- Continuous subcutaneous (s.c.) apomorphine infusion is an effective therapy for Parkinson's disease (PD), but a limitation is the formation of troublesome s.c. nodules. Various chemically non-identical apomorphine formulations are available. Anecdotal experiences have suggested that shifting from one of these (Apo-Go PumpFill®; apoGPF) to another (Apomorphine PharmSwed®; apoPS) may influence the occurrence and severity of s.c. nodules. We, therefore, followed 15 people with advanced PD (median PD-duration, 15 years; median "off"-phase Hoehn and Yahr, IV) on apoGPF and with troublesome s.c. nodules who were switched to apoPS. Data were collected at baseline, at the time of switching, and at a median of 1, 2.5, and 7.3 months post-switch. Total nodule numbers (P < 0.001), size (P < 0.001), consistency (P < 0.001), skin changes (P = 0.058), and pain (P ≤ 0.032) improved over the observation period. PD severity and dyskinesias tended to improve and increase, respectively. Apomorphine doses were stable, but levodopa doses increased by 100 mg/day. Patient-reported apomorphine efficacy tended to increase and all participants remained on apoPS throughout the observation period; with the main patient-reported reason being improved nodules. These observations suggest that patients with s.c. nodules caused by apoGPF may benefit from switching to apoPS in terms of s.c. nodule occurrence and severity. Alternatively, observed benefits may have been due to the switch itself. As nodule formation is a limiting factor in apomorphine treatment, a controlled prospective study comparing local tolerance with different formulations is warranted.
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2. |
- Lanemo Myhrinder, Anna, 1975-, et al.
(author)
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A new perspective : molecular motifs on oxidized LDL, apoptotic cells, and bacteria are targets for chronic lymphocytic leukemia antibodies
- 2008
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In: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 111:7, s. 3838-3848
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Journal article (peer-reviewed)abstract
- The restricted immunoglobulin (Ig) repertoire found in B-cell chronic lymphocytic leukemia (CLL) implies a role for antigen(s) in the leukemogenesis. The nature of the antigens has, however, not been characterized, although examples of autoantigens have been demonstrated. We have analyzed a panel of 28 CLL cell lines and primary cultures, producing monoclonal Ig with different Ig heavy-chain variable region gene usage and mutational status, including several complementarity determining region 3 homology subset members. Using mass-spectrometry, immunoassays, or protein macroarrays, we have discovered novel antigens binding to CLL Igs. These antigens included cytoskeletal proteins vimentin, filamin B, and cofilin-1, but also phosphorylcholine-containing antigens (eg, Streptococcus pneumoniae polysaccharides and oxidized low-density lipoprotein [oxLDL]). Additional new antigens identified were cardiolipin and proline-rich acidic protein-1. Remarkably, these antigens represent molecular motifs exposed on apoptotic cells/blebs and bacteria, and several CLL Igs bound to apoptotic Jurkat cells. In conclusion, these intriguing data, showing a limited target structure recognition, indicate that CD5(+) CLL B cells are derived from a cell compartment that produces "natural antibodies," which may be instrumental in elimination and scavenging of apoptotic cells and pathogenic bacteria.
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3. |
- Myhrinder, Anna Lanemo, 1975-, et al.
(author)
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Molecular characterization of neoplastic and normal "sister" lymphoblastoid B-cell lines from chronic lymphocytic leukemia
- 2013
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In: Leukemia and Lymphoma. - : Informa UK Limited. - 1042-8194 .- 1029-2403. ; 54:8, s. 1769-1779
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Journal article (peer-reviewed)abstract
- Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5 + B-cells carrying auto/xeno-antigen-reactive B-cell receptors (BCRs) and multiple innate pattern-recognition receptors, such as Toll-like receptors and scavenger receptors. Integration of signals from BCRs with multiple surface membrane receptors determines whether the cells will be proliferating, anergic or apoptotic. To better understand the role of antigen in leukemogenesis, CLL cell lines producing monoclonal antibodies (mAbs) will facilitate structural analysis of antigens and supply DNA for genetic studies. We present here a comprehensive genotypic and phenotypic characterization of available CLL and normal B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n = 17). Authenticity and verification studies of CLL-patient origin were done by IGHV sequencing, fluorescence in situ hybridization (FISH) and DNA/short tandem repeat (STR) fingerprinting. Innate B-cell features, i.e. natural Ab production and CD5 receptors, were present in most CLL cell lines, but in none of the normal LCLs. This panel of immortalized CLL-derived cell lines is a valuable reference representing a renewable source of authentic Abs and DNA.
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4. |
- Rosén, Anders, et al.
(author)
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Lymphoblastoid cell line with B1 cell characteristics established from a chronic lymphocytic leukemia clone by in vitro EBV infection
- 2012
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In: Oncoimmunology. - : Informa UK Limited. - 2162-4011 .- 2162-402X. ; 1:1, s. 18-27
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Journal article (peer-reviewed)abstract
- Chronic lymphocytic leukemia (CLL) cells express the receptor for Epstein-Barr virus (EBV) and can be infected in vitro. Infected cells do not express the growth-promoting set of EBV-encoded genes and therefore they do not yield LCLs, in most experiments. With exceptional clones, lines were obtained however. We describe a new line, HG3, established by in vitro EBV-infection from an IGHV1-2 unmutated CLL patient clone. All cells expressed EBNA-2 and LMP-1, the EBV-encoded genes pivotal for transformation. The karyotype, FISH cytogenetics and SNP-array profile of the line and the patient's ex vivo clone showed biallelic 13q14 deletions with genomic loss of DLEU7, miR15a/miR16-1, the two micro-RNAs that are deleted in 50% of CLL cases. Further features of CLL cells were: expression of CD5/CD20/CD27/CD43 and release of IgM natural antibodies reacting with oxLDL-like epitopes on apoptotic cells (cf. stereotyped subset-1). Comparison with two LCLs established from normal B cells showed 32 genes expressed at higher levels (> 2-fold). Among these were LHX2 and LILRA. These genes may play a role in the development of the disease. LHX2 expression was shown in self-renewing multipotent hematopoietic stem cells, and LILRA4 codes for a receptor for bone marrow stromal cell antigen-2 that contributes to B cell development. Twenty-four genes were expressed at lower levels, among these PARD3 that is essential for asymmetric cell division. These genes may contribute to establish precursors of CLL clones by regulation of cellular phenotype in the hematopoietic compartment. Expression of CD5/CD20/CD27/CD43 and spontaneous production of natural antibodies may identify the CLL cell as a self-renewing B1 lymphocyte.
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5. |
- Danielsson, Marie, et al.
(author)
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Mini-seedlings of Picea abies are less attacked by Hylobius abietis than conventional ones : Is plant chemistry the explanation?
- 2008
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In: Scandinavian Journal of Forest Research. - : Informa UK Limited. - 0282-7581 .- 1651-1891. ; 23:4, s. 299-306
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Journal article (peer-reviewed)abstract
- The pine weevil, Hylobius abietis (L.), is a major pest in conifer reforestation areas in the Palaearctic region. Size and chemistry of the seedlings may explain the damage rates in plantations. The performance of 10-week containerized seedlings (mini-seedlings) was compared with 1-year-old conventional seedlings of Norway spruce, Picea abies (L.), in a field experiment in central Sweden. After 2 years the weevil damage was lower for the mini-seedlings than for the conventional seedlings (3.5 vs 55%). After 3 years, the overall survival was 82 and 75%, respectively. Weevil damage was the main cause of mortality for conventional seedlings, whereas mini-seedlings mainly died from drought. Volatiles of the two seedling types were compared by solid-phase microextraction-gas chromatography -mass spectrometry (SPME-GC-MS). Unwounded mini-seedlings and conventional seedlings differed in their compositions of monoterpenes and sesquiterpenes. Miniseedlings mainly emitted limonene, known to be repellent to the pine weevil. When wounded, green leaf volatiles were released by mini-seedlings while the pine weevil attractant alpha-pinene was released by conventional seedlings. Volatiles may partly explain the mini-seedlings' resistance against weevil attack. Further studies are needed to clarify how long this miniseedling effect remains.
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6. |
- Engdahl, Johan, et al.
(author)
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Multicentre, national, investigator-initiated, randomised, parallel-group, register-based superiority trial to compare extended ECG monitoring versus standard ECG monitoring in elderly patients with ischaemic stroke or transient ischaemic attack and the effect on stroke, death and intracerebral bleeding : the AF SPICE protocol
- 2023
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In: BMJ Open. - : BMJ Publishing Group Ltd. - 2044-6055. ; 13:11
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Journal article (peer-reviewed)abstract
- Introduction: Atrial fibrillation (AF) is a major risk factor for ischaemic stroke and transient ischaemic attack (TIA), and AF detection can be challenged by asymptomatic and paroxysmal presentation. Long-term ECG monitoring after ischaemic stroke or TIA is recommended by all major societies in cardiology and cerebrovascular medicine as a secondary prophylactic measure. However, data on stroke reduction are lacking, and the recommendations show significant diversity.Methods and analysis: AF SPICE is a multicentre, national, investigator-initiated, randomised, parallel-group, register-based trial comparing extended ECG monitoring versus standard ECG monitoring in patients admitted with ischaemic stroke or TIA, with a composite endpoint of stroke, all-cause-mortality and intracerebral bleeding. Patients aged ≥ 70 years without previous AF will be randomised 1:1 to control (standard ECG monitoring) or intervention (extended ECG monitoring). In the control arm, patients will undergo 48±24 hours (ie, a range of 24-72hours) of continuous ECG monitoring according to national recommendations. In the intervention arm, patients will undergo 14+14 days of continuous ECG monitoring 3months apart using an ECG patch device, which will provide an easy-accessed, well-tolerated 14-day continuous ECG recording. All ECG patch recordings will be read in a core facility. In cases of AF detection, oral anticoagulation will be recommended if not contraindicated. A pilot phase has been concluded in 2022, which will transcend into the main trial during 2023-2026, including approximately 30 stroke units. The sample size was calculated to be 3262 patients. The primary outcome will be collected from register data during a 36-month follow-up.Ethics and dissemination: Ethical approval has been provided by the Swedish Ethical Review Authority, reference 2021-02770. The trial will be conducted according to the ethical principles of the Declaration of Helsinki and national regulatory standards. Positive results from the study have the potential for rapid dissemination in clinical practice. Trial registration number NCT05134454.
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7. |
- Georgoudaki, Anna-Maria, et al.
(author)
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Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis
- 2016
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In: Cell Reports. - : Elsevier BV. - 2211-1247. ; 15:9, s. 2000-2011
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Journal article (peer-reviewed)abstract
- Tumors are composed of multiple cell types besides the tumor cells themselves, including innate immune cells such as macrophages. Tumor-associated macrophages (TAMs) are a heterogeneous population of myeloid cells present in the tumor microenvironment (TME). Here, they contribute to immunosuppression, enabling the establishment and persistence of solid tumors as well as metastatic dissemination. We have found that the pattern recognition scavenger receptor MARCO defines a subtype of suppressive TAMs and is linked to clinical outcome. An anti-MARCO monoclonal antibody was developed, which induces anti-tumor activity in breast and colon carcinoma, as well as in melanoma models through reprogramming-TAM-populations to a pro-inflammatory phenotype and increasing tumor immunogenicity. This anti-tumor activity is dependent on the inhibitory Fc-receptor, Fc gamma RIIB, and also enhances the efficacy of checkpoint therapy. These results demonstrate that immunotherapies using antibodies designed to modify myeloid cells of the TME represent a promising mode of cancer treatment.
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8. |
- Gunnarsson, Cecilia, 1970-, et al.
(author)
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17β-hydroxysteroid dehydrogenases involved in local oestrogen synthesis have prognostic significance in breast cancer
- 2005
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In: British Journal of Cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 92:3, s. 547-552
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Journal article (peer-reviewed)abstract
- The 17β-hydroxysteroid dehydrogenase (17HSD) enzymes are involved in the local regulation of sex steroids. The 17HSD type 1 enzyme catalyses the interconversion of the weak oestrone (E1) to the more potent oestradiol (E2), whereas 17HSD type 2 catalyses the oxidation of E2 to E1. The aim of this study was to correlate the expression of these enzymes in the tumour with the recurrence-free survival of tamoxifen-treated breast cancer patients. We used real-time reverse transcriptase PCR to investigate the mRNA expression of 17HSD types 1 and 2 in tumour samples from 230 postmenopausal patients. For the patients with oestrogen receptor (ER)-positive breast cancer, we found a statistically significant positive correlation between recurrence-free survival and expression of 17HSD type 2 (P = 0.026). We examined the ratio of 17HSD types 2 and 1, and ER-positive patients with low ratios showed a significantly higher rate of recurrence than those with higher ratios (P = 0.0047), ER positive patients with high expression levels of 17HSD type 1 had a significantly higher risk for late relapse (P = 0.0051). The expression of 17HSD types 1 and 2 in breast cancer differs from the expression of these enzymes in normal mammary gland, and this study indicates that the expression has prognostic significance in breast cancer.
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9. |
- Hagell, Peter, et al.
(author)
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Apomorphine formulation influences subcutaneous complications in continuous apomorphine pump therapy for Parkinson’s disease
- 2017
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In: Movement Disorders.
-
Conference paper (other academic/artistic)abstract
- Objective: To explore if the occurrence and severity of subcutaneous (sc) nodules is influenced by the pharmaceutical formulation of apomorphine used for sc infusion in advanced Parkinson’s disease (PD).Background: Apomorphine infusion is an effective therapy in advanced PD, but a limitation is troublesome sc nodules. Various chemically non-identical apomorphine formulations are available. Anecdotal clinical experience has suggested that shifting from one of these (Apo-Go PumpFill; apoGPF) to another (Apomorphine PharmSwed; apoPS, developed in Sweden) may influence the occurrence and severity of sc nodules.Methods: In this multicenter open-label prospective observational study, 15 people with advanced PD (mean PD- duration, 13.4 years; median Hoehn & Yahr, IV) on apoGPF since a mean of 2.1 years and with troublesome sc nodules were switched to apoPS. Ongoing interventions to treat existing nodules (ultrasound, massage, Hirudoid cream) continued, and apomorphine as well as other drugs was managed according to clinical routines. Data were collected between May 2015 and March 2017; at baseline, at the time of switching (about 2 weeks later), and up to 1.7-4.2 (mean, 2.5) months post-switch follow-up. Primary outcomes were total nodule numbers, size (mm diameter for the 5 worst nodules), consistency (scored 0-3 for the 5 worst nodules), and associated skin changes (scored 0-4 for the 5 worst nodules) and pain (scored 0-5). Patients also rated their perceived PD severity and motor complications (UPDRS IV). Patient preferences 5-12 months post-switch (2-9 months after follow-up) were also recorded.Results: Apomorphine and L-dopa doses did not change over the observation period (P≥0.400). Baseline nodule numbers (7.4 vs. 4.6; P<0.003), size (92.9 vs. 54.1 mm; P=0.016), consistency (11 vs. 5; P=0.003), skin changes (3 vs. 1.5; P=0.205), and average pain (1 vs. 0; P=0.020) improved 11 weeks post-switch. Patient-reported PD severity (P=0.020) and motor fluctuations improved (P=0.051), whereas dyskinesias tended to increase (P=0.205). At 5-12 months post-switch, 13 patients had decided to remain on apoPS; mainly due to improved nodules.Conclusions: These observations suggest that apoPS may have a better safety profile compared to apoGPF in terms of sc nodule occurrence and severity. There is a need for larger, randomized controlled studies for firmer conclusions.
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10. |
- Hagell, Peter, et al.
(author)
-
Apomorphine formulation influences subcutaneous complications in continuous apomorphine pump therapy for Parkinson’s disease
- 2017
-
Conference paper (other academic/artistic)abstract
- Objective: To explore if the occurrence and severity of subcutaneous (sc) nodules is influenced by the pharmaceutical formulation of apomorphine used for sc infusion in advanced Parkinson’s disease (PD). Background: Apomorphine infusion is an effective therapy in advanced PD, but a limitation is troublesome sc nodules. Various chemically non-identical apomorphine formulations are available. Anecdotal clinical experience has suggested that shifting from one of these (Apo-Go PumpFill; apoGPF) to another (Apomorphine PharmSwed; apoPS, developed in Sweden) may influence the occurrence and severity of sc nodules. Methods: In this multicenter open-label prospective observational study, 15 people with advanced PD (mean PD- duration, 13.4 years; median Hoehn & Yahr, IV) on apoGPF since a mean of 2.1 years and with troublesome sc nodules were switched to apoPS. Ongoing interventions to treat existing nodules (ultrasound, massage, Hirudoid cream) continued, and apomorphine as well as other drugs was managed accordingto clinical routines. Data were collected between May 2015 and March 2017; at baseline, at the time of switching (about 2 weeks later), and up to 1.7-4.2 (mean, 2.5) months post-switch follow-up. Primary outcomes were total nodule numbers, size (mm diameter for the 5 worst nodules), consistency (scored 0-3 for the 5 worst nodules), and associated skin changes (scored 0-4 for the 5 worst nodules) and pain (scored 0-5). Patients also rated their perceived PD severity and motor complications (UPDRS IV). Patient preferences 5-12 months post-switch (2-9 months after follow-up) were also recorded. Results: Apomorphine and L-dopa doses did not change over the observation period (P≥0.400). Baseline nodule numbers (7.4 vs. 4.6; P<0.003), size (92.9 vs. 54.1 mm; P=0.016), consistency (11 vs. 5; P=0.003), skin changes (3 vs. 1.5; P=0.205), and average pain (1 vs. 0; P=0.020) improved 11 weeks post-switch. Patient-reported PD severity (P=0.020) and motor fluctuations improved (P=0.051), whereas dyskinesias tended to increase (P=0.205). At 5-12 months post-switch, 13 patients had decided to remain on apoPS; mainly due to improved nodules. Conclusions: These observations suggest that apoPS may have a better safety profile compared to apoGPF in terms of sc nodule occurrence and severity. There is a need for larger, randomized controlled studies for firmer conclusions.
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11. |
- Hagell, Peter, et al.
(author)
-
Apomorphine formulation may influence subcutaneous complications from continuous subcutaneous apomorphine infusion in Parkinson's disease
- 2020
-
In: Journal of Neurology. - : D. Steinkopff-Verlag. - 0340-5354 .- 1432-1459. ; 267:11, s. 3411-3417
-
Journal article (peer-reviewed)abstract
- Continuous subcutaneous (s.c.) apomorphine infusion is an effective therapy for Parkinson's disease (PD), but a limitation is the formation of troublesome s.c. nodules. Various chemically non-identical apomorphine formulations are available. Anecdotal experiences have suggested that shifting from one of these (Apo-Go PumpFill®; apoGPF) to another (Apomorphine PharmSwed®; apoPS) may influence the occurrence and severity of s.c. nodules. We, therefore, followed 15 people with advanced PD (median PD-duration, 15 years; median "off"-phase Hoehn and Yahr, IV) on apoGPF and with troublesome s.c. nodules who were switched to apoPS. Data were collected at baseline, at the time of switching, and at a median of 1, 2.5, and 7.3 months post-switch. Total nodule numbers (P < 0.001), size (P < 0.001), consistency (P < 0.001), skin changes (P = 0.058), and pain (P ≤ 0.032) improved over the observation period. PD severity and dyskinesias tended to improve and increase, respectively. Apomorphine doses were stable, but levodopa doses increased by 100 mg/day. Patient-reported apomorphine efficacy tended to increase and all participants remained on apoPS throughout the observation period; with the main patient-reported reason being improved nodules. These observations suggest that patients with s.c. nodules caused by apoGPF may benefit from switching to apoPS in terms of s.c. nodule occurrence and severity. Alternatively, observed benefits may have been due to the switch itself. As nodule formation is a limiting factor in apomorphine treatment, a controlled prospective study comparing local tolerance with different formulations is warranted.
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12. |
- Hallgren, Karin, 1957-, et al.
(author)
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Musik och politik i svenskt 1970-tal : musikspridning, jämlikhet och kommersialism på de kungliga teatrarna och SVT?
- 2017
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In: Musikforskning idag. - : Luleå University of Technology. ; , s. 26-27
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Conference paper (other academic/artistic)abstract
- Under 1960- och 70-talen var debatten om kulturfrågor intensiv i Sverige. Beslutet om kulturpolitikens inriktning 1974 fick stor betydelse för kulturlivets organisering under de kommande decennierna. Den nya kulturpolitiken byggde bland annat på idéer om behovet av att sprida kultur jämlikt till alla invånare i Sverige, samt att ”Kulturpolitiken skall motverka kommersialismens negativa verkningar inom kulturområdet” (Kulturutredningen 1995:62). Samtidigt hade svenska grupper inom populärmusiken stora internationella framgångar, med ABBA som det mest framgångsrika och mest kända exemplet. Kulturdebatten blev polariserad, med tydliga motsättningar mellan förespråkare för kulturpolitikens grundläggande värderingar å ena sidan och ”den kommersiella musikens” företrädare å den andra.Denna motsättning är fokus för vår studie. Hur påverkades olika institutioner av den nya kulturpolitiken och av de aktuella idéerna i debatten? I denna studie görs en jämförelse mellan några konkreta yttringar inom musiklivet. Exempel ges från de traditionstyngda scenerna Kungliga Operan och Dramaten i Stockholm samt från SVT:s verksamhet, där programmet ”Alternativ festival” från 1975 används som en fallstudie. Förutom utvalda program och föreställningar bygger studien på arkivmaterial från institutionerna. Vi använder en komparativ metod och genomför föreställningsanalys utifrån teatervetenskapliga metoder.1970-talets kulturdebatt inom musikens område är till vissa delar beskriven och analyserad i tidigare forskning (se exv. Arvidsson 2008; Thyrén 2009) men en jämförelse mellan institutioner av detta slag har inte gjorts tidigare. Här finns en möjlighet att visa hur kulturlivet i stort påverkades av de nya idéerna och hur både hög- och lågutbildade gavs tillfälle att ta del av detta.
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14. |
- Hellqvist, Eva, 1978-
(author)
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Antigen interaction with B cells in two proliferative disorders : CLL and MGUS
- 2010
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Doctoral thesis (other academic/artistic)abstract
- The aim of the work presented in this thesis was to elucidate B cell interaction with antigen in the two B cell proliferative disorders chronic lymphocytic leukemia (CLL) and monoclonal gammopathy of undetermined significance (MGUS). In the first part we investigated the antigen specificity of CLL cells and characterized Epstein-Barr virus (EBV)-transformed CLL cell lines with regard to phenotype and genotype. The second part consists of studies on the antigen presenting capacity of myelin protein zero (P0) specific MGUS B cells and their relation to T cells and development of polyneuropathy.The aim of the work presented in this thesis was to elucidate B cell interaction with antigen in the two B cell proliferative disorders chronic lymphocytic leukemia (CLL) and monoclonal gammopathy of undetermined significance (MGUS). In the first part we investigated the antigen specificity of CLL cells and characterized Epstein-Barr virus (EBV)-transformed CLL cell lines with regard to phenotype and genotype. The second part consists of studies on the antigen presenting capacity of myelin protein zero (P0) specific MGUS B cells and their relation to T cells and development of polyneuropathy.CLL cells are considered antigen experienced and different patient-derived CLL cells expressing B cell receptors (BCR) with highly homologous antigen binding sites are believed to have been selected by a common antigen at some point during the leukemogenesis. In paper I we investigated the antigen specificity of CLL-cell derived antibodies (Abs) with various IGHV gene usage and stereotyped BCR subset belonging. Identified CLL antigens included vimentin, filamin B, cofilin-1, proline rich acidic protein-1, cardiolipin, oxidized low density lipoprotein and Streptococcus pneumoniae capsular polysaccharides. Many of the CLL Abs studied displayed an oligo- or polyreactive antigen binding pattern and the identified antigens were either associated with apoptotic cells or microbial infection. This is similar to what has been described for innate natural antibodies, possibly indicating that CLL cells are derived from a natural-antibody- producing B cell population. Further characterization of CLL homology subset-2 antigen specificity showed binding to glands in human gastric mucosa corpus tissue sections for a CLL homology subset-2 Ab with HCDR3 motif-1, suggesting that this CLL subset recognize an autoantigen much like the CLL Abs tested in Paper I.Characterization of EBV-transformed CLL and normal lymphoblastoid cell lines (LCLs) in paper II showed that eight of the CLL cell lines were verified to be of authentic neoplastic origin. Indication for a biclonal CLL was found in two of the cell lines and two of the presumably normal LCLs turned out to represent the malignant CLL clone. For three cell lines no conclusive evidence for CLL origin could be found emphasizing the importance of verifying the identity of cell lines used in research.
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15. |
- Hellqvist, Eva, 1978-, et al.
(author)
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IGHV3-21 stereotyped subset-2 chronic lymphocytic leukemia cells make autoantibodies that bind to an 11.5kDa gastric mucosal antigen
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Other publication (other academic/artistic)abstract
- Background: The immunoglobulin heavy chain variable region (IGHV) gene mutational status is an important prognostic factor in chronic lymphocytic leukemia (CLL). Patients with mutated IGHV genes show significantly longer survival than unmutated cases. CLL patients with mutated IGHV3-21 genes, however, have a shorter survival compared to other mutated CLL cases. Recently, we showed that CLL cells react with oxidized epitopes exposed on apoptotic cells and bacteria. The gastric mucosal reactivity was of special interest to further characterize in detail. Methods: We collected corpus biopsies from seven Helicobacter pylori (H.p.)+ study subjects withnon-atrophic gastritis, six subjects with H.p.- atrophic gastritis, eight subjects with H.p.+ atrophicgastric and from eight controls without gastritis. The binding pattern of CLL subset-2 IGHV3-21antibodies was analyzed by immunohistochemistry and the antigen was biochemically purified byaffinity chromatography. Results: The subset-2 IGHV3-21 Abs bound to mucosal glands in 18 of 29 cases regardless of H.p.status or diagnosis. It also showed staining of connective tissue in all of the biopsies. The antigen waspurified and a protein of 11.5 kDa MW was isolated. Conclusion: The results from this study indicate that IGHV3-21 subset-2 CLL Abs bind an 11.5kDaprotein present in gastric mucosal glands or connective tissue. This autoantigen has no associationwith H.p. since it is present in normal, non-atrophic H.p.+ and atrophic H.p.+/H.p.- gastric mucosafrom corpus. The exact nature of the 11.5 kDa protein is currently under detailed structure massspectrometry analysis in order to reveal whether bacterial mimicry is the mechanism behind theinduction of this particular autoantibody.
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16. |
- Hellqvist, Eva, 1978-, et al.
(author)
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Myelin protein zero is naturally processed in IgM MGUS B cells : Aberrant triggering of patient T cells
- 2010
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In: Haematologica. - : Ferrata Storti Foundation (Haematologica). - 0390-6078 .- 1592-8721. ; 95:4, s. 627-636
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Journal article (peer-reviewed)abstract
- Background and Objectives: Monoclonal gammopathy of undetermined significance (MGUS) of IgM isotype is a condition with clonally expanded B cells, recently suggested having an infectious origin. MGUS is frequently associated with polyneuropathy and antibodies against myelin protein zero (P0), whereas the role of the T cells remains largely unknown. Here we have analyzed P0-specific B cells, as antigen-presenting cells, and their capacity to activate T helper cells. Design and Methods: We used a well-characterized MGUS-derived B cell line, TJ2, expressing anti-P0 IgM. The ability of TJ2 cells to bind, endocytose, process, and present P0 was investigated by receptor-clustering and immunofluorescence. The activation of P0-specific autologous T cells was studied by measuring IL2 and IFNγ with flow cytometry, immunobeads, and ELISPOT. Results: Surface-receptor clustering and endocytosis of receptor-ligand (IgM/P0) complexes were pronounced after P0 exposure. Naturally processed or synthetic P0 peptide (194-208)-pulsed TJ2 cells significantly induced IL2 secretion from autologous T cells compared to control antigen pulsed cells (p<0 .001). The numbers of IFNγ producing T helper cells, including CD4+/CD8+ cells, were also significantly increased (p=0.0152). Affinity-isolated naturally processed myelin peptides were potent IFNγ stimulators for autologous PBMCs, but not for control PBMCs. Interpretation and conclusions: We show for the first time that myelin P0 is naturally processed in IgM MGUS B cells, acting as aberrant antigen-presenting cells in activation of patients T helper cells. Our findings cast new light on the important role of autoreactive P0-specific B cells in he induction of the pathogenic T cell responses found in nerve lesions of MGUS patients with peripheral neuropathy.
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17. |
- Kjellander Hellqvist, Eva, 1965-
(author)
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‘At the end of the storm, there’s a golden sky’ : The importance of an English football anthem for the Swedish fans
- 2022
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In: Journal of fandom studies. - : Intellect Ltd.. - 2046-6692 .- 2046-6706. ; 10:1, s. 57-71
-
Journal article (peer-reviewed)abstract
- The purpose of this article is to discuss the significance of Liverpool Football Club’s (LFC) anthem ‘You’ll Never Walk Alone’ (‘YNWA’) for Swedish fans of LFC. The song has been played before home matches at the Anfield stadium since the 1960s, and via the media, it has spread all over the world. We can therefore talk about a kind of satellite supporters who can be involved no matter where in the world they are. Based on a survey conducted with help from Liverpool FC Official Supporters Club Sweden, 378 people (355 men and 23 women) answered questions about their relationship to the city of Liverpool and the Liverpool football team, how long they have been fans and why they chose LFC, and, finally, their connection to ‘YNWA’. The results show that the Swedish fans feel a great sense of belonging to both the team and the city of Liverpool and that the song is very important to them even in everyday life. For the fans in the survey, ‘YNWA’ unites them with the team and fans from all over the world while separating themselves from other fans and football clubs with other anthems. The song thus becomes larger than itself. Identity and performances are important concepts on which the article is based.
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20. |
- Kjellander Hellqvist, Eva, 1965-
(author)
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Danskultur–Folkkultur
- 2014
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In: Musikforskning idag, Växjö, 11-13 juni 2014.
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Conference paper (other academic/artistic)
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21. |
- Kjellander Hellqvist, Eva, 1965-
(author)
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Den erotiske spelmannen
- 2008. - 1
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In: En värld för sig själv. - Växjö : Växjö university press. - 9789176365861 ; , s. 319-337
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Book chapter (other academic/artistic)
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22. |
- Kjellander Hellqvist, Eva, 1965-
(author)
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Den erotiske spelmannen : Näcken i sägen och ballad
- 2007
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In: Musikvetenskap idag - Konferens den 12-14 juni 2007 i Stockholm.
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Conference paper (other academic/artistic)abstract
- Många av de teman och övernaturliga väsen som förekommer i de naturmytiska visorna hittar vi även i våra folksägner. Näcken är ett exempel på ett sådant väsen. Han uppehåller sig kring eller i vatten och han var farlig för alla och envar som kom i kontakt med honom. Män och kvinnor kom i kontakt med näcken på olika platser och under olika omständigheter. Sägnerna berättar om näcken som läromästare i musik, och det är männen som får lära sig den konsten, ofta genom att lämna något i utbyte för kunskapen. För kvinnornas del var han riktigt farlig och han lockade dem till både det ena och det andra, att röra sig utanför hemmet var förenat med stor fara.I balladerna gestaltas han på ett annat sätt. Spelmanstemat existerar inte i balladerna utan det är den erotiske manspersonen som är huvudtemat. I balladen Agneta och hafsmannen berättas det om hur Agneta dras ner i djupet av näcken och tvingas leva med honom där.Det finns många intressanta kopplingar mellan sägnerna och balladerna och min avsikt är att föra fram förslag på gemensamma beståndsdelar samt att diskutera de skillnader som föreligger.
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23. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
”Don't stand so close to me” : Musik som medel för att hantera en pandemi
- 2021
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In: Populär : Nordic journal for popular culture research. - Lund : Lund University. ; 1:2, s. 1-8
-
Journal article (peer-reviewed)abstract
- Eva Kjellander Hellqvist bidrag diskuterar musikens betydelse för välmående, under en tid då det inte har varit möjligt att gå på konserter, fotbollsmatcher eller på annat sätt uppleva musik eller evenemang. Kjellander Hellquist diskuterar hur musik och känslor kopplas ihop med tidigare erfarenheter och hur utövandet av fanskap påverkats under pandemin.
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24. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Fanskap som identitet
- 2009
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In: Musikvetenskap idag – Musikvetenskaplig konferens i Lund 9–11 juni 2009. ; , s. 8-8
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Conference paper (other academic/artistic)abstract
- I mitt forskningsprojekt kommer jag att undersöka hur vuxna fans ser på sitt fanskap och sina idoler. Med utgångspunkt i tre olika populärmusikaliska genrer: rock, dansband och hårdrock vill jag undersöka hur sex utvalda fans fanskap ser ut. Det jag framför allt kommer att fokusera på är identiteten som fan. Vad innebär det att vara ett vuxet fan? Vilka konsekvenser får fanskapet för vardagslivet? Får det konsekvenser för arbetslivet? Jag är även intresserad av hur man lyssnar och på vad. Är det musik eller text eller både och? Vad är det som gör att man fastnar för en artist eller genre och håller fast vid den år efter år? Hur ser en jämförelse ut mellan de olika genrerna? Vilka likheter och skillnader finns det och hur ser de ut? Jag vill också titta på det sociokulturella lärande som sker och hur detta lärande ser ut.Syftet är därför att:• genom att analysera sex fans musikaliska berättelser, med utgångspunkt i deras fanskap, vill jag undersöka hur fanskapet påverkat och påverkar deras liv• skapa större förståelse för vuxna fans• dokumentera en typ av fanskap som troligtvis kommer att dö utDe artister vars fans jag undersöker är Kiss (hårdrock), Status Quo (rock) och Lasse Stefanz (dansband). Urvalet är gjort efter kriterierna att det ska vara olika genrer och att artisterna ska ha varit aktiva sedan genrens början och därmed haft tid att skaffa sig en ordentlig fanbas. Artisterna ska även ha en stor publik i Sverige och fortfarande vara aktiva. Genom att välja ut ett amerikanskt (Kiss), ett engelskt (Status Quo) och ett svenskt (Lasse-Stefanz) band kan jag även undersöka om tillgängligheten har någon betydelse för fansen.
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25. |
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26. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Fanskap som identitet : fler preliminära resultat
- 2011
-
In: Musikforskning idag. - : Uppsala universitet. ; , s. 12-13
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Conference paper (other academic/artistic)abstract
- I mitt forskningsprojekt har jag undersökt hur sex vuxna fans ser på sitt fanskap och sina idoler. Med utgångspunkt i tre olika populärmusikaliska genrer: rock (Status Quo), dansband (Lasse Stefanz) och hårdrock (Kiss). Syftet är Att generera en teori kring vuxna fans och deras identitet som fans.SocialiseringHos informanterna sker det en primär socialisering in i en genre genom familjen eller, i ett fall, genom media. Fyra av informanterna har socialiserats in i rockgenren medan de andra två socialiserats in i föräldrarnas kristna musik. Det alla har gemensamt är att de vid 20Dårs ålder, oavsett hur lång tid det tog för individen att hitta sitt favoritband, alla var fans till de band undersökningen handlar om. För Sanne och Axel, båda Kissfans, och för Erik (Lasse Stefanz) gick det fort. De var alla fans redan när de var runt tio år. För Rickard och Tina, båda Status Quofans, dröjde det till dess att de var i gymnasieåldern, och för Kicki (Lasse Stefanz) till hon frigjorde sig från sin familj och flyttade hemifrån när hon var 19 år. Att Sanne och Axel fastnar tidigt kan bero på att Kiss var ett väletablerat band i Sverige när de växte upp. För Eriks del är orsaken att brodern var anställd av Lasse Stefanz och därmed var bandet etablerat i Eriks värld. Status Quo var som störst i Sverige när Rickard och Tina var i gymnasieåldern och de spelades en del på radion. Kicki är född i slutet av 50-talet och Lasse Stefanz slog inte genom nationellt förrän 1983, trots att de funnits sedan 1967. Med andra ord kan man säga att en etablering av bandet i Sverige var mycket viktig för att ett fanskap skulle kunna uppstå. För att höra eller se sina favoriter var de hänvisade till skivor eller de få program som spelades på radio eller visades på teve, och för att få information om banden fick de köpa någon av de enstaka ungdomstidningar som fanns. Föräldrarna förmedlade sin musiksmak till barnen och syskon och kamrater förmedlade sin.
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27. |
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28. |
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29. |
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30. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Jag och mitt fanskap : vad musik kan göra för människor
- 2013
-
Doctoral thesis (other academic/artistic)abstract
- This dissertation aims to find further understanding of how people with special interest in a certain artist utilise music and the fact that they are fans in their everyday lives. I have opted to study two fans included in each group selected for this study: Kiss, Status Quo and Lasse Stefanz, one male and one female fan belonging to each respective group. I have worked according to grounded theory as a method, and through an analysis of their musical life stories, I have attempted to identify why they became fans and how them being fans has affected them in their lives.Four categories, fandom as: a marker of identity, socialisation, a form of self therapy and a pseudo religion and the core category authenticity usage show the results of the study. The categories show that to a large extent it all comes down to the musical identity of these people, i.e. the identity of being a fan, and their experiences of being fans. They have been socialised into a specific genre, which has meant increased interest in a specific artist. Family, media and friends have all played a part in this socialisation. The informants have developed cultural competence as concerns their idols, although they have also gained the subcultural capital resources required in order to come across as credible fans. Various kinds of experiences offer meaning and nourish the fans. Security and stability in everyday lives are also contributing factors to them being fans and the music offers them something that they are unable to acquire from elsewhere. They have established different strategies in order to be able to be fans, one of these being legitimacy. A vital part of this legitimacy consists in them viewing the bands as authentic, i.e. important.
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31. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Jag och mitt fanskap
- 2012
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In: Musik och Samhälle IV, Malmö Högskola och ABF.
-
Conference paper (other academic/artistic)
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32. |
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33. |
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34. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Musik och idoler : om att vara fans till lågstatusband
- 2014. - 1
-
In: Coda. - Malmö : Kira. - 9789198092196 ; , s. 119-128
-
Book chapter (pop. science, debate, etc.)abstract
- Alla människor har någon gång i sitt liv en idol eller i alla fall någon de ser upp till, en förebild. Detta är ett fenomen som i mångt och mycket hör ungdomen till, men också något som kan följa med genom hela livet. Under de tidiga tonåren börjar individen utveckla en egen musiksmak och detta sker ofta genom influenser från familj, media och kamrater och hänger intimt samman med identitetsprocessen. I min avhandling Jag och mitt fanskap- vad musik kan betyda för människor (2013) visar jag hur viktigt det är även för vuxna att ha idoler, och vad det innebär för den enskilda personen. I denna artikel, som utgår från avhandlingen, handlar det om sex fans till lågstatusbanden Kiss (hårdrock, bildades 1973), Status Quo (boogie-woogierock, bildades 1962) och Lasse Stefanz (dansband, bildades 1967), alltså band med låg autenticitet som inte tillhör den outtalade populärmusikaliska kanon som finns. Denna kanon bestäms i stor utsträckning av journalister och forskare, det vill säga dem som oftast kommer med olika smakomdömen och som väljer vilka artister som är värda att skriva om. För fansen till dessa lågstatusband är det dock oproblematiskt då de ser artisterna som äkta och väderfulla, oavsett hur andra uppfattar dem. Problemen som uppstår är av annan karaktär och handlar om hur fansen hanterar det faktum att omvärlden har en annan syn på artisterna än vad de själva har. Det har alltså inget att göra med huruvida banden är bra eller dåliga, det är upp till var och en att bedöma, och för fansen är de självklart väldigt bra. Syftet med denna artikel är därför att visa på de olika strategier fansen kan anta för att hantera det faktum att de är fans till lågstatusband.För en fördjupning kring detta hänvisar jag till min avhandling Jag och mitt fanskap- vad musik kan betyda för människor (2013).
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38. |
- Kjellander Hellqvist, Eva, 1965-
(author)
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Smak för musik
- 2015
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In: Musik och samhälle VII, Lunds universitet och ABF.
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Conference paper (other academic/artistic)
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39. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Smak för musik?
- 2016
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In: M&STE: Elektronisk tidskrift för konferensen Musik & Samhälle. - 2002-4622. ; 1, s. 52-60
-
Journal article (pop. science, debate, etc.)
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40. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Spellistan som husapotek
- 2018
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In: Musik och Samhälle IX. - Lund : Lund University.
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Conference paper (other academic/artistic)
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41. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
Spellistan som husapotek
- 2019. - 6
-
In: M&STE: Elektronisk tidskrift för konferensen Musik & Samhälle. - Lund : Lund University. ; :6, s. 14-19
-
Journal article (pop. science, debate, etc.)
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42. |
- Kjellander Hellqvist, Eva, 1965-
(author)
-
“Together we can rock´n´roll”, Status Quo – a study of the career of a rock band
- 2006
-
In: Musikvetenskap idag.
-
Conference paper (other academic/artistic)abstract
- The main purpose of this essay is on one hand to try to find and explain the formula of success of the rock group Status Quo, and on the other to elucidate and explain the foundation and essence in the music made by Status Quo, but also to study how, if and why the foundation and essence have changed over the years. The method of investigation is partly based on a musical analysis of three albums from three different periods: Hello! (1973), Ain´t Complaining (1988) and Heavy Traffic (2002), and partly on a sociological study of the group’s career between 1962 and today, 2005.I run to the conclusion that the essence of Status Quo is the boogie-woogie comp on Fender guitar by Rick Parfitt, the riff on Francis Rossis´ Fender guitar in combination with the base, the piano and the drums. This has been the essence through all the years, even though it was more difficult to apprehend it during the 1980s. The group’s musical formula seems to be an introduction presented by a riff, a verbal verse and chorus. Then there is a guitar solo, where the song is stripped down to one or two instruments, only to be rebuilt before the last chorus and finally completed with an outroduktion. The simplicity is also a big part of the music of Status Quo and the main parts of the songs are based around three or four chords. I believe these aspects to be the basic foundations in the music made by Status Quo and have, depending on the fluctuations, remained the same during the four decades that Status Quo has been active. Due to lots of touring and heavy marketing Status Quo has become a people’s band, and I believe that this popularity along with the musical foundation is the main formula for their success.
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43. |
- Kjellander Hellqvist, Eva, 1965-, et al.
(author)
-
Ämnesspråk i klassrummet
- 2018
-
In: Musikforskning idag 2018, Uppsala 13–15 juni. - Uppsala : Uppsala University. ; , s. 8-8
-
Conference paper (other academic/artistic)abstract
- Som en del i ett större projekt om ämnesspråk vid Centrum för ämnesdidaktisk forskning vid Linnéuniversitetet (CÄHL), studerar vi ämnesspråket i högstadiets klassrumsundervisning i musik. Studien är deskriptiv till sin karaktär, och syftet är primärt att beskriva hur ämnesspråket i musikundervisningen på högstadiet gestaltar sig. Studien baseras på filminspelningar av sex lektioner i musik runt om i södra Sverige.Två centrala utgångspunkter för studien är att det finns ett ämnesspråk i musikundervisningen och att det är multimodalt till sin karaktär. Det vill säga att ämnesspråket inte enkelt kan beskrivas som något som sägs (verbalt) eller gestaltas genom olika skriftliga strategier (exv. på tavlan, i textmaterial och liknande).Vid Musikforskning i dag vill vi ge en överblick över vilka olika former av ”språk” (vardagligt,skolspråk, ämnesspråk, ”musikerspråk”, vetenskapligt språk) som förekommer i de lektioner vihar dokumenterat. Därefter vill vi beskriva de delar av materialet som vi har kategoriserat som ämnesspråk och ge exempel på hur vi arbetat med dem utifrån ett multimodalt perspektiv i vår analys. Mer specifikt analyserar vi det insamlade materialet utifrån ett social-semiotiskt perspektiv med fokus på vilka semiotiska resurser som lärarna använder sig av i sin undervisning.Studiens preliminära resultat visar att olika semiotiska resurser såsom tal, skrift, sång, instrumentspelande, gestik och liknande, ofta samverkar för att framföra ett innehåll i undervisningen. Dessutom samsas musikundervisningens ämnesspråk, med språk från andra domäner, såsom skolspråk, vardagsspråk, ”musikerspråk”, vetenskapligt språk.
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44. |
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45. |
- Klasson, Anna, et al.
(author)
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Positive MRI Enhancement in THP-1 Cells with Gd2O3 Nanoparticles
- 2008
-
In: Contrast Media and Molecular Imaging. - : Wiley. - 1555-4309 .- 1555-4317. ; 3:3, s. 106-111
-
Journal article (peer-reviewed)abstract
- There is a demand for more efficient and tissue-specific MRI contrast agents and recent developments involve the design of substances useful as molecular markers and magnetic tracers. In this study, nanoparticles of gadolinium oxide (Gd2O3) have been investigated for cell labeling and capacity to generate a positive contrast. THP-1, a monocytic cell line that is phagocytic, was used and results were compared with relaxivity of particles in cell culture medium (RPMI 1640). The results showed that Gd2O3-labeled cells have shorter T1 and T2 relaxation times compared with untreated cells. A prominent difference in signal intensity was observed, indicating that Gd2O3 nanoparticles can be used as a positive contrast agent for cell labeling. The r1 for cell samples was 4.1 and 3.6 s-1 mm-1 for cell culture medium. The r2 was 17.4 and 12.9 s-1 mm-1, respectively. For r1, there was no significant difference in relaxivity between particles in cells compared to particles in cell culture medium, (pr1 = 0.36), but r2 was significantly different for the two different series (pr2 = 0.02). Viability results indicate that THP-1 cells endure treatment with Gd2O3 nanoparticles for an extended period of time and it is therefore concluded that results in this study are based on viable cells.
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46. |
- Lindström, Anders, et al.
(author)
-
Mini seedlings : a new forest regeneration system
- 2005
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In: The Thin Green Line: A Symposium on the State of the Art in Reforestation. - Thunder Bay, Ontario.
-
Conference paper (other academic/artistic)abstract
- Swedish forest tree nurseries produce approximately 300–350 million seedlings annually for outplanting. Most seedlings (80%) are containerised i.e. they are produced in small containers or pots that are filled with a growing media.
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47. |
- Lindström, Anders, et al.
(author)
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Miniplantor
- 2005
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In: ELMIAS Skogsmässa. - Jönköping.
-
Conference paper (other academic/artistic)
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48. |
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49. |
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50. |
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