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- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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- Bergman, Penny, 1982, et al.
(author)
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The pleasant heat? Evidence for thermal-emotional implicit associations occurring with semantic and physical thermal stimulation
- 2015
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In: Cognitive Neuroscience. - : Informa UK Limited. - 1758-8928 .- 1758-8936. ; 6:1, s. 24-30
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Journal article (peer-reviewed)abstract
- The association between thermal and emotional experiences in interpersonal relations is intuitively apparent and has been confirmed by previous studies. However, research has not yet elucidated whether such an association is grounded in mental processes occurring at an intrapersonal (internal) level. In two experiments we examined whether the thermal-emotional associations can be observed at an intrapersonal level. We looked at the speed and accuracy of stimuli categorization. Experiment 1 examined the implicit semantic association between temperature (warm versus cold) and emotional valence (positive versus negative). Experiment 2 examined the association between experience of physical temperature and emotional valence. In both experiments warm-positive/cold-negative associations were demonstrated. These results suggest a conceptual and perceptual mapping in the mental representation of emotion and temperature, which occurs at an intrapersonal level, and which might serve as the ground to the interpersonal thermal-emotional interactions.
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| 4. |
- Brooks, Jas, et al.
(author)
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Sharing and Experiencing Hardware and Methods to Advance Smell, Taste, and Temperature Interfaces
- 2023
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In: CHI EA '23: Extended Abstracts of the 2023 CHI Conference on Human Factors in Computing Systems. - : Association for Computing Machinery (ACM). - 9781450394222
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Conference paper (peer-reviewed)abstract
- There has been a monumental push from the CHI community to bring more human senses to interactive devices. This trend is significant because we use all our senses in everyday interactions but only an extremely narrow subset when interacting with computers. This workshop focuses on bringing together researchers to advance some of the most challenging senses to embed into interfaces, but arguably the most exciting: smell, taste, and temperature. To integrate these modalities into interfaces, researchers not only use methods from traditional mechanics or haptics (e.g., pumps, heating pads, etc.) but must also acquire tacit skills and understandings from psychophysics, neuroscience, anatomy, and chemistry (e.g., receptor signaling pathways or food chemistry). This demo-based workshop provides a platform to come together and bring their demonstrations, experiments, and hardware to experience, discuss, and advance the field.
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- Tajadura, Ana, 1979, et al.
(author)
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Whole-body vibration influence sound localization in the median plane.
- 2007
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In: 10th Annual International Workshop on Presence, Barcelona, Spain, October 2007..
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Conference paper (peer-reviewed)abstract
- The perceived location of events occurring in a mediated environment modulates the users’ understanding and involvement in these events. Previous research has shown that when spatially discrepant information is available at various sensory channels, the perceived location of unisensory events might be altered. Tactile “capture” of audition has been reported for lateral sounds. The present study investigates whether auditory localization on the median plane could be altered by concurrent whole-body vibration. Sounds were presented at the front or the back of participants, in isolation or together with vibrations. Subjects made a three alternative forced choice regarding their perceived location of sound (“front”, “back” or “center”). Results indicate that vibrations synchronous with sound affected subjects’ sound localization, significantly reducing the accuracy on front sound localization in favor of “back” and “center” responses. This research might have implications for the design of multimodal environments, especially for those aiming at creating a sense of presence or inducing affective experiences in users.
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