| 1. |
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| 2. |
- Brannstrom, J, et al.
(author)
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Helicobacter pylori stimulates DNA synthesis in a small intestinal cell line in vitro
- 1998
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In: Digestion. - : S. Karger AG. - 0012-2823 .- 1421-9867. ; 59:1, s. 33-39
-
Journal article (peer-reviewed)abstract
- <b>Background:</b> <i>Helicobacter pylori</i>, which causes gastritis and peptic ulcer, seems to be an important factor in the pathogenesis of gastric cancer and MALT lymphoma. Thus our aim was to examine whether <i>H. pylori</i> influences DNA synthesis in epithelial cells in vitro. <b>Methods:</b> Sonicated and water extracts of <i>H. pylori</i> (cytotoxic strains NCTC 11637, 88-23 and A5, and a noncytotoxic isogenic mutant of A5, A5 vac A) were diluted to a final concentration of 1/1,000, 1/100, 1/50 and 1/10. Water extracts of <i>Escherichia coli</i> were used as reference. IEC-6 cells were incubated during 24 h with fragments of <i>H. pylori</i> or extracts of the concentrations described above. The cells were labeled with <sup>3</sup>H-methylthymidine for 4 h and processed for autoradiography. DNA synthesis was evaluated by the labeling index (LI). <b>Results:</b> The LI% of controls was 15.6 ± 5.1%. All the water extracts and sonicated strains of <i>H. pylori</i> increased the LI% in a dose-dependent manner (p < 0.001). The highest concentrations of the sonicated strains tended to reduce the LI%, although these values were still higher than those of the control group. The water extracts of <i>E. coli</i> increased the LI% in a dose-dependent manner (p < 0.0001). <b>Conclusion:</b> <i>H. pylori</i> stimulates DNA synthesis in epithelial cells in vitro, but no association was found with the presence of cytotoxin production. Our results suggest that hitherto unknown components of <i>H. pylori</i> may contribute to the increase in cell proliferation observed in gastritis and to the development of MALT lymphoma and gastric cancer.
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| 3. |
- Drevinge, Christina, 1983, et al.
(author)
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Perilipin 5 is protective in the ischemic heart
- 2016
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In: International Journal of Cardiology. - : Elsevier BV. - 0167-5273 .- 1874-1754. ; 219, s. 446-454
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Journal article (peer-reviewed)abstract
- Background: Myocardial ischemia is associated with alterations in cardiac metabolism, resulting in decreased fatty acid oxidation and increased lipid accumulation. Here we investigate how myocardial lipid content and dynamics affect the function of the ischemic heart, and focus on the role of the lipid droplet protein perilipin 5 (Plin5) in the pathophysiology of myocardial ischemia. Methods and results: We generated Plin5(-/-) mice and found that Plin5 deficiency dramatically reduced the triglyceride content in the heart. Under normal conditions, Plin5(-/-) mice maintained a close to normal heart function by decreasing fatty acid uptake and increasing glucose uptake, thus preserving the energy balance. However, during stress or myocardial ischemia, Plin5 deficiency resulted in myocardial reduced substrate availability, severely reduced heart function and increased mortality. Importantly, analysis of a human cohort with suspected coronary artery disease showed that a common noncoding polymorphism, rs884164, decreases the cardiac expression of PLIN5 and is associated with reduced heart function following myocardial ischemia, indicating a role for Plin5 in cardiac dysfunction. Conclusion: Our findings indicate that Plin5 deficiency alters cardiac lipid metabolism and associates with reduced survival following myocardial ischemia, suggesting that Plin5 plays a beneficial role in the heart following ischemia. (C) 2016 The Authors. Published by Elsevier Ireland Ltd.
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| 4. |
- Ekman, Stina, et al.
(author)
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Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses
- 2019
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In: Equine Veterinary Journal. - : Wiley. - 0425-1644 .- 2042-3306. ; 51:5, s. 674-680
-
Journal article (peer-reviewed)abstract
- Background Molecular serum markers that can identify early reversible osteoarthritis (OA) in horses are lacking. Objectives We studied serum concentrations of a novel cartilage oligomeric matrix protein (COMP) neo-epitope in horses subjected to short-term exercise and with acute lameness. The effects of circadian rhythm and age were also evaluated. Study design Longitudinal studies in healthy horses and cross-sectional comparison of lame and non-lame horses. Methods Sera were collected from five horses before and after short-term interval exercise and during full-day box rest. Sera from 32 acutely lame horses were used to evaluate age-related effects. Independent samples from control horses (n = 41) and horses with acute lameness (n = 71) were included. COMP neo-epitope concentrations were analysed using custom-developed inhibition ELISAs validated for equine serum. The presence of COMP neo-epitope was delineated in healthy and osteoarthritic articular cartilage with immunohistochemistry. Results COMP neo-epitope concentrations decreased after speed training but returned to baseline levels post-exercise. No correlations between age and serum COMP neo-epitope concentrations were found (r = 0.0013). The mean (+/- s.d.) serum concentration of COMP neo-epitope in independent samples from non-lame horses was 0.84 +/- 0.38 mu g/mL, and for lame horses was 5.24 +/- 1.83 mu g/mL (P<0.001). Antibodies against COMP neo-epitope did not stain normal articular cartilage, but intracytoplasmic staining was found in superficial chondrocytes of mild OA cartilage and in the extracellular matrix of moderately osteoarthritic cartilage. Main limitations ELISA was based on polyclonal antisera rather than a monoclonal antibody. There is a sex and breed bias within the groups of horses, also it could have been of value to include horses with septic arthritis and tendonitis and investigated joint differences. Conclusions This COMP neo-epitope can be measured in sera, and results indicate that it could be a biomarker for pathologic fragmentation of cartilage in connection with acute joint lameness.
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| 5. |
- Hawton, K, et al.
(author)
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Relation between attempted suicide and suicide rates among young people in Europe.
- 1998
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In: Journal of Epidemiology and Community Health. - : BMJ. - 0143-005X .- 1470-2738. ; 52:3, s. 191-4
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Journal article (peer-reviewed)abstract
- Rates of attempted suicide and suicide in the young covary. The recent increase in attempted suicide rates in young male subjects in several European countries could herald a further increase in suicide rates.
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| 6. |
- Hultén, A, et al.
(author)
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Recommended care for young people (15-19 years) after suicide attempts in certain European countries.
- 2000
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In: European Child and Adolescent Psychiatry. - 1018-8827 .- 1435-165X. ; 9:2, s. 100-8
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Journal article (peer-reviewed)abstract
- Data on recommended care for young people aged 15-19 years after attempted suicide from nine European research centres during the period 1989-1992 were analysed in terms of gender, history of previous suicide attempt and methods used. Altogether 438 suicide attempts made by 353 boys and 1,102 suicide attempts made by 941 girls were included. Analyses of the total data from all centres showed that young people with a history of previous suicide attempt and those using violent methods had significantly higher chance of being recommended aftercare than first-time attempters or those choosing self-poisoning. There were no significant differences of being recommended care between genders. Logistic regression analyses of the material were performed and the results were similar. Both having previous attempted suicide (odds ratio 2.0, 95% CI 1.53-2.61) and using "hard" methods (odds ratio 1.71, 95% CI 1.49-1.96) were significantly associated with increased possibility of being recommended aftercare. When individual centres were analysed, large disparities of recommended care after suicide attempts were found and there were no uniform criteria of recommending care for young suicide attempters in Europe.
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| 7. |
- Ljungvall, K, et al.
(author)
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Early post-natal exposure to low dose oral di(2ethylhexyl) phthalate affects the peripheral LH-concentration in plasma, but does not affect mating behavior in the post-pubertal boar
- 2006
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In: Reproductive Toxicology. - : Elsevier. - 0890-6238 .- 1873-1708. ; 21:2, s. 160-166
-
Journal article (peer-reviewed)abstract
- In a split-litter design experiment, boars were exposed orally three times weekly to 300 mg/kg of di(2-ethylhexyl) phthalate (DEHP) between 3 and 7 weeks of age. Post-puberty, i.e. at 6 months of age the effects on endocrinology and mating behavior were examined. The response to stimulation with a synthetic GnRH-analogue at 9 months of age resulted initially in lower concentration of LH in the exposed animals, compared to the control animals. We did not find any effects of DEHP on the mating behavior. Also, the effects of DEHP during the treatment period on the plasma concentrations of testosterone, oestradiol and LH were examined. During the exposure period there was a transient decrease in plasma concentrations of LH in the control group, which did not occur in the boars exposed to DEHR The data suggest that DEHP in low repeated oral doses causes lasting effects on the hypothalamus-pituitary-gonadal axis. (c) 2005 Elsevier Inc. All rights reserved.
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| 8. |
- Adepu, Saritha, et al.
(author)
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Salivary biglycan-neo-epitope-BGN262: A novel surrogate biomarker for equine osteoarthritic sub-chondral bone sclerosis and to monitor the effect of short-term training and surface arena
- 2023
-
In: Osteoarthritis and Cartilage Open. - : Elsevier BV. - 2665-9131. ; 5:2
-
Journal article (peer-reviewed)abstract
- Objective: We aimed to delineate a novel soluble Biglycan Neo-epitope-BGN262 in saliva from young reference and osteoarthritic horses in conjunction with the influence of short-term training exercise, riding surface hardness, circadian rhythm, and feeding on its soluble levels. Design: A custom-made inhibition ELISA was used for the quantification of BGN262 in saliva. Cohort 1: A cross-sectional study comprising reference (N = 19) and OA horses (N = 9) with radiographically classified subchondral bone sclerosis. Receiver operating characteristic curve analysis was performed to evaluate the robustness of BGN262. Cohorts 2 (N = 5) & 3 (N = 7): Longitudinal studies of sampling during a short-term training exercise (sand-fibre) and a cross-over design of short-training exercise on 2 different riding arenas (sand and sand-fibre), respectively. Capillary western immunoassay was used to determine the BGN262 molecular size in a selection of saliva samples collected from cohort 1. Results: Cohort 1: Salivary BGN262 levels were significantly higher in the OA group. The Receiver operating characteristic curve analysis showed an area under the curve of 0.8304 [0.6386 to 1.022], indicating a good separation from the reference group. Cohorts 2 & 3: Salivary BGN262 levels significantly changed during the exercise on sand and sand-fibre arena, with a trend towards higher levels for sand-fibre. The size of the BGN262 fragment determined by Capillary western assay was 18 kDa. Conclusions: The data presented show saliva BGN262 levels as a novel biomarker in evaluating the influence of exercise, and interaction with riding arenas alongside assessing osteoarthritis severity.
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| 9. |
- Andersson, Linda, 1973, et al.
(author)
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Deficiency in perilipin 5 reduces mitochondrial function and membrane depolarization in mouse hearts.
- 2017
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In: The international journal of biochemistry & cell biology. - : Elsevier BV. - 1878-5875 .- 1357-2725. ; 91:Pt A, s. 9-13
-
Journal article (peer-reviewed)abstract
- Myocardial triglycerides stored in lipid droplets are important in regulating the intracellular delivery of fatty acids for energy generation in mitochondria, for membrane biosynthesis, and as agonists for intracellular signaling. Previously, we showed that deficiency in the lipid droplet protein perilipin 5 (Plin5) markedly reduces triglyceride storage in cardiomyocytes and increases the flux of fatty acids into phospholipids. Here, we investigated whether Plin5 deficiency in cardiomyocytes alters mitochondrial function. We found that Plin5 deficiency reduced mitochondrial oxidative capacity. Furthermore, in mitochondria from Plin5((-/)(-)) hearts, the fatty acyl composition of phospholipids in mitochondrial membranes was altered and mitochondrial membrane depolarization was markedly compromised. These findings suggest that mitochondria isolated from hearts deficient in Plin5, have specific functional defects.
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| 10. |
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| 11. |
- Bergh, U, et al.
(author)
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Maximal oxygen uptake and muscle fiber types in trained and untrained humans.
- 1978
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In: Medicine and science in sports. - 0025-7990. ; 10:3, s. 151-4
-
Journal article (peer-reviewed)abstract
- Maximal oxygen uptake (Vo2 max) was determined in 138 male and 41 female human subjects and muscle fiber composition (gastrocnemius and vastus lateralis) in 53 of the males. Highest values for Vo2 max were 7.38 1 x min-1 and 4.341 x min-1 in males and females, respectively. In relation to body weight the highest values were 94 and 77 ml x (kg x min)-1. Athletes participating in endurance events had very high Vo2 max and predominantly slow twitch (ST) fiber populations whereas weight lifters attained rather low values for Vo2 max and had a higher percentage of fast twitch (FT) fibers. Among subjects with the same fiber composition, Vo2 max was higher in the athletes than in the moderately trained. All groups taken together demonstrated a positive relationship between Vo2 max and the relative number of ST fibers (r = 0.67). For endurance and strength athletes r = 0.72 and for the moderately trained r = 0.34, both correlation coefficients being significant.
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| 12. |
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| 13. |
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| 14. |
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| 15. |
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| 16. |
- Björkman, G., et al.
(author)
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Att genomföra och avrapportera projektarbetsuppgifter på delkurs PK:1
- 1977
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Reports (other academic/artistic)abstract
- Detta är en rapport om hur man bedriver och avrapporterar projektarbete på kurs PK:1 i informationsbehandling. Rapporten beskriver de olika momenten i projektarbetet och innehåller dessutom regler och anvisningar för detta arbete. Rapporten försöker även förmedla tips och erfarenheter från tidigare terminer beträffande kurs PK:1
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| 17. |
- Dohse, Dirk, et al.
(author)
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Paths academic scientists take to entrepreneurship : Disaggregating direct and indirect influences
- 2021
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In: Managerial and Decision Economics. - : Wiley. - 0143-6570 .- 1099-1468. ; 42:7, s. 1740-1753
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Journal article (peer-reviewed)abstract
- Based on information from a large sample of German researchers and using business ownership and nascent entrepreneurship as alternative indicators of academic entrepreneurship, we use mediation analysis to analyze the direct effects of researchers' entrepreneurship attitudes, age, gender, and citizenship as well as the related indirect influences. Industrial cooperation, industry consulting, and patenting are used as alternative mediator variables. Focusing first on the overall drivers of academic entrepreneurship, the results show differences in the drivers of business ownership and nascent entrepreneurship. With regard to age, we find positive and significant indirect effects; they are negative for females; and positive for German citizens. The identification of direct and indirect channels of influence on academic entrepreneurship is the main contribution of this work.
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| 18. |
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| 19. |
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| 20. |
- Enroth, H, et al.
(author)
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Diagnostic accuracy of a rapid whole-blood test for detection of Helicobacter pylori
- 1997
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In: Journal of clinical microbiology. - : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 35:10, s. 2695-2697
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Journal article (peer-reviewed)abstract
- In this study, we evaluated a rapid whole-blood test, BM-test Helicobacter pylori, for detection of H. pylori infection in 144 and 48 patients with other gastrointestinal symptoms and with gastric cancer, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of the test correlated well with the standards used for the calculation, i.e., serology by enzyme-linked immunosorbent assay or culture and histology.
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| 21. |
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| 22. |
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| 23. |
- Goel, Rajeev K., et al.
(author)
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Academic leadership and commercial activities at research institutes : German evidence
- 2018
-
In: Managerial and Decision Economics. - : Wiley. - 0143-6570. ; 39:5, s. 601-609
-
Journal article (peer-reviewed)abstract
- This paper focuses on entrepreneurship by academic leaders. With the use of patents, inventions, and spin-offs to measure commercialization, and directors, research group leaders, and business owners as academic leaders, results, using a sample of more than 2,500 German researchers, show differences across academic leaders and commercialization. Findings for spin-offs are different from those for patents and inventions. Academic leaders in sciences were more likely to commercialize. Doctoral degrees helped patents by business owners and spin-offs by group leaders, whereas female business owners and female group leaders faced challenges patenting and inventing, respectively. For business owners, age increased the likelihood of patenting but lowered spin-offs.
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| 24. |
- Goel, Rajeev K., et al.
(author)
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Academics' entrepreneurship propensities and gender differences
- 2015
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In: Journal of Technology Transfer. - : Springer Science and Business Media LLC. - 0892-9912 .- 1573-7047. ; 40:1, s. 161-177
-
Journal article (peer-reviewed)abstract
- Using survey data from a large public research organization, this study examines entrepreneurship propensities of academic researchers, focusing on gender differences. Although sample means of female and male propensities toward entrepreneurship are fairly similar, regression estimates show significant gender differences in the association of several factors with propensities to start businesses. In particular, prior record of researchers' patenting and institutional leadership promote tendencies towards entrepreneurship among male researchers, but not among female researchers. Also, unlike the male scientists, doctoral degrees and preference for open access of research results do not significantly influence the entrepreneurial attitudes of female researchers. The results for the full sample are similar to those for the male subsample, with a negative coefficient on the variable identifying females.
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| 25. |
- Goel, Rajeev K., et al.
(author)
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Drivers of innovation productivity of academic researchers through career advancement
- 2020
-
In: Journal of Technology Transfer. - : Springer Science and Business Media LLC. - 0892-9912 .- 1573-7047. ; 45:2, s. 414-429
-
Journal article (peer-reviewed)abstract
- This paper studies the innovation productivity of academic researchers across their career advancement. Taking patents and invention disclosures as indicators of innovation productivity and using survey data from a large German public research organization, researchers’ productivity is examined during early career and early to mid-career across various dimensions, including years since employment, years since terminal degree and age. The extant literature has primarily focused on publishing productivity of researchers. Results show that early leadership position consistently enhances innovation productivity during the life cycle. However, the effects of the other dimensions differ somewhat. Interestingly, having a doctoral degree enables one to overcome the disadvantages of being a female researcher, and German citizen increases productivity for the overall sample and for early to mid-career researchers.
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| 26. |
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| 27. |
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| 28. |
- Goel, Rajeev K., et al.
(author)
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Innovation by foreign researchers : relative influences of internal versus external human capital
- 2019
-
In: Journal of Technology Transfer. - : Springer Science and Business Media LLC. - 0892-9912 .- 1573-7047.
-
Journal article (peer-reviewed)abstract
- Innovation productivity of expatriate researchers and human capital that empowers them is an important issue; however, with a scarcity of relevant data, formal empirical evidence has been limited. This paper uses a rich dataset of foreign researchers at a leading German research institution to study the drivers of their innovation productivity (measured by patents and invention disclosures). Another contribution is that we examine the relative effectiveness of internal (institution-specific) and external human capital. These findings are compared to the behavior of native researchers. Results, based on a survey sample of nearly 1000 foreign researchers and taking account of the interdependence between invention disclosures and patenting, show that internal administrative leadership positions (especially group leaders) enhanced propensities to innovate, while purely academic positions (professor or guest researcher) did not positively impact innovation. With regard to external human capital, foreign researchers who were business owners or nascent entrepreneurs were more likely to innovate. Internal (institutional) experience mattered more than industry experience. Doctoral degrees seemed relatively more effective for patenting than for invention disclosures. No significant gender differences in innovation productivity were found. In contrast, while many of the influences were similar for domestic researchers, they experienced positive innovation spillovers from being directors, but domestic female researchers faced special innovation challenges. Finally, domestic professors, unlike foreign professors, did not experience negative tendencies towards innovation.
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| 29. |
- Goel, Rajeev K., et al.
(author)
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Nascent entrepreneurship and inventive activity: a somewhat new perspective
- 2013
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In: Journal of Technology Transfer. - : Springer Science and Business Media LLC. - 0892-9912 .- 1573-7047. ; 38:4, s. 471-485
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Journal article (peer-reviewed)abstract
- This paper focuses on the nexus between nascent entrepreneurship (NE) and inventive activity. It questions how NE affects inventive activity (including innovation and patenting) while analyzing the views and predictions that have used patenting as an indicator of entrepreneurial behavior. Using data on German researchers and controlling for their personal, professional and institutional attributes, the findings show that NE increases both patenting and innovation. Implications for technology policy are discussed.
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| 30. |
- Goel, Rajeev K., et al.
(author)
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Risk attitudes, patenting and invention disclosures by academic researchers
- 2019
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In: Journal of Technology Transfer. - : Springer Science and Business Media LLC. - 0892-9912 .- 1573-7047. ; 44:1, s. 155-166
-
Journal article (peer-reviewed)abstract
- This paper examines the effect of risk attitudes on innovation behavior of academic researchers. Specifically, using two alternate measures of self-reported risk attitudes, we test the hypothesis that aversion to risk would negatively affect revelation of information in innovations. Results based on a sample of more than 2500 German researchers across both measures of risk aversion show that, consistent with intuition, risk aversion has a significant negative effect on invention disclosures, but not on patenting.
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| 31. |
- Goel, Rajeev K., et al.
(author)
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What drives academic patentees to bypass TTOs? Evidence from a large public research organisation
- 2018
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In: Journal of Technology Transfer. - : Springer Science and Business Media LLC. - 0892-9912 .- 1573-7047. ; 43:1, s. 240-258
-
Journal article (peer-reviewed)abstract
- This paper provides insights into the behavior of academic patentees who choose to bypass in-house Technology Transfer Offices (TTOs). TTOs have gained favor in recent years as academic institutions have tried to increase commercialization of their inventions. Using a large sample of researchers at a leading German public research organisation (PRO), results show that patentees in physical and life sciences, those with doctoral degrees, and those with greater job experience are more likely to bypass TTOs. Different forms of industry interactions, including working in industry, industry cooperation and industry consulting, all make TTO-bypassing more likely, with some interesting differences across gender. Further, as expected, academics favoring free public access to their research are less likely to bypass TTOs. On the other hand, internal leadership position as a research group leader, German citizenship and risk attitudes do no exert significant influences. Implications for technology transfer policies are discussed.
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| 32. |
- Goel, Rajeev K., et al.
(author)
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Who instigates university–industry collaborations? University scientists versus firm employees
- 2017
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In: Small Business Economics. - : Springer Science and Business Media LLC. - 0921-898X .- 1573-0913. ; 48:3, s. 503-524
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Journal article (peer-reviewed)abstract
- While evidence on the causes and effects of university–industry interaction is abundant, little is known about how, and particularly by whom, such interaction is instigated in the first place and subsequently managed. In this paper, we investigate which mode of collaboration (joint research, contract research, consulting, in-licensing, or informal contacts) is more likely to be initiated and managed by firm employees versus by university scientists. Moreover, we are interested in the differences between small and large firms to see whether initiation and management are affected by firm size. Using a sample of 833 German manufacturing firms, our results indicate that university scientists typically start collaborations with industry, while firm employees would take over the management of projects. Results vary markedly between small and large firms, with university scientists having somewhat higher difficulties initiating collaborations with large firms than with small firms.
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| 33. |
- Gritli Linde, Amel, 1959, et al.
(author)
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Effects of suramin on polyamine metabolism in B16 murine melanoma cells
- 1998
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In: Anticancer Res. - 0250-7005. ; 18:2A, s. 855-62
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Journal article (peer-reviewed)abstract
- Polyamines and their biosynthetic enzymes, such as ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC), are crucial for normal and neoplastic cell growth and differentiation. Suramin inhibits the growth of several tumor cells by affecting various intracellular targets, but its effects on polyamines are not known. In this study, the effects of suramin on some parameters of polyamine metabolism in B16 melanoma cells were investigated in vitro. Suramin increased cellular ODC activity and ODC mRNA levels, whereas the drug was directly inhibitory to the enzyme. AdoMetDC was not affected. Cellular putrescine levels were enhanced by suramin, whereas spermidine and spermine pools were unaltered. Cells cultured in the presence of suramin showed decreased cellular polyamine transport, but no direct inhibitory effect on the polyamine transporter could be found. Fluorescence spectroscopy demonstrated a direct interaction between suramin and spermine. It may be concluded that suramin affects polyamine metabolism, and that its effects in some respects are opposite to those of alpha-difluoromethylomithine (DFMO), a specific inhibitor of ODC.
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| 34. |
- Hallmans, Göran, et al.
(author)
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Rye, lignans and human health
- 2003
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In: Proceedings of the Nutrition Society. - 0029-6651 .- 1475-2719. ; 62:1, s. 193-9
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Journal article (peer-reviewed)abstract
- Rye bran contains a high content not only of dietary fibre, but also of plant lignans and other bioactive compounds in the so-called dietary fibre complex. Blood concentrations of lignans such as enterolactone have been used as biomarkers of intake of lignan-rich plant food. At present,evidence from studies in human subjects does not warrant the conclusion that rye, whole grains orphyto-oestrogens protect against cancer. Some studies, however, have pointed in that direction,especially in relation to cancers of the upper digestive tract. A number of prospective epidemiological studies have clearly shown a protective effect of wholegrain cereals against myocardial infarctions. A corresponding protective effect against diabetes and ischaemic stroke(brain infarct) has also been demonstrated. It seems reasonable to assume that these protective effects are associated with one or more factors in the dietary fibre complex.
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| 35. |
- Hiukka, A, et al.
(author)
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ApoCIII-Enriched LDL in Type 2 Diabetes Displays Altered Lipid Composition, Increased Susceptibility for Sphingomyelinase and Increased binding to Biglycan.
- 2009
-
In: Diabetes. - : American Diabetes Association. - 1939-327X .- 0012-1797. ; 58:9, s. 2018-2026
-
Journal article (peer-reviewed)abstract
- Objective- Apolipoprotein CIII (apoCIII) is an independent risk factor for cardiovascular disease, but the molecular mechanisms involved are poorly understood. Here, we investigated potential proatherogenic properties of apoCIII-containing LDL from hypertriglyceridemic patients with type 2 diabetes. Research design and methods - LDL was isolated from controls and subjects with type 2 diabetes, and from apoB transgenic mice. LDL-biglycan binding was analyzed with a solid-phase assay using immunoplates coated with biglycan. Lipid composition was analyzed with mass spectrometry. Hydrolysis of LDL by sphingomyelinase was analyzed after labeling plasma LDL with [(3)H]sphingomyelin. ApoCIII isoforms were quantified after isoelectric focusing. Human aortic endothelial cells were incubated with desialylated apoCIII or with LDL enriched with specific apoCIII isoforms. Results- We showed that enriching LDL with apoCIII only induced a small increase in LDL-proteoglycan binding, and this effect was dependent on a functional Site A in apoB100. Our findings indicated that intrinsic characteristics of the diabetic LDL other than apoCIII per se are responsible for further increased proteoglycan binding of diabetic LDL with high endogenous apoCIII, and we showed alterations in the lipid composition of diabetic LDL with high apoCIII. We also demonstrated that high apoCIII increased susceptibility of LDL to hydrolysis and aggregation by SMase. In addition, we demonstrated that sialylation of apoCIII increased with increasing apoCIII content, and that sialylation of apoCIII was essential for its proinflammatory properties. Conclusions- We have demonstrated a number of features of apoCIII-containing LDL from hypertriglyceridemic patients with type 2 diabetes that could explain the proatherogenic role of apoCIII.
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| 36. |
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| 37. |
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| 38. |
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| 39. |
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| 40. |
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| 41. |
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| 42. |
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| 43. |
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| 44. |
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| 45. |
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| 46. |
- Karlsson, J, et al.
(author)
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LDH isozymes in skeletal muscles of endurance and strength trained athletes.
- 1975
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In: Acta Physiologica Scandinavica. - 0001-6772 .- 1365-201X. ; 93:2, s. 150-6
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Journal article (peer-reviewed)abstract
- Muscle biopsy samples were obtained from arm and leg muscles of endurance and strength trained athletes, respectively. Total LDH activity as well as occurrence and activity of LDH isozymes were determined. Comparing the results from the athletes with those from non-trained subjects with corresponding fibre compositions, it was found that the endurance athletes had a lower total LDH activity, a higher relative activity of the most heart-specific isozymes. LDH (1 + 2), and, on electrophoretic separation, a complete absence of LDH (4 + 5) in both arm and leg muscles. As compared to the untrained material the strength trained athletes tended to have a higher total LDH activity, a similar distribution of relative isozyme activities, and, in the leg muscles, a strong electrophoretic band corresponding to LDH 5, the most skeletal muscle specific isozyme.
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| 47. |
- Ljungvall, K, et al.
(author)
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Delayed effects on plasma concentration of testosterone and testicular morphology by intramuscular low-dose di(2-ethylhexyl)phthalate or oestradiol benzoate in the prepubertal boar
- 2005
-
In: Theriogenology. - : Elsevier. - 0093-691X .- 1879-3231. ; 64:5, s. 1170-1184
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Journal article (peer-reviewed)abstract
- The immediate and delayed effects of prepubertal exposure to di(2-ethylhexyl)phthalate (DERP) or oestradiol benzoate on the plasma concentrations of testosterone, oestradiol and LH, as well as testicular morphology were examined in prepubertal boars. In a split litter design experiment, prepubertal boars were intramuscularly exposed to DEHP, oestradiol or vehicle during five weeks, starting at six weeks of age. The dose of DEHP was 50 mg/kg of bodyweight twice weekly, which is in the same range as recently used oral doses in rodents. Oestradiol-benzoate was administered at 0.25 mg/kg of bodyweight twice weekly. One set of animals was examined immediately after the exposure, and the other set was examined at an age of 7.5 months. During the exposure period concentrations of LH in plasma were lower (p = 0.02) in the oestradiol-treated animals than in the control group. In the group exposed to oestradiol, the relative to the body weight of the testicles tended to be lower (p = 0.07) than control immediately after five weeks of exposure, and the relative to the body weight of the seminal vesicles tended to be lower (p = 0.05) than control at 7.5 months of age. In the DEHP-exposed group an elevated (p = 0.005) concentration of testosterone and increased (p = 0.04) area of the Leydig cells in the testicles compared to the control group were seen at 7.5 months of age. These data suggest that DEHP early in life causes delayed effects on the reproductive system in the adult. (c) 2005 Elsevier Inc. All rights reserved.
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