| 1. |
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| 2. |
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| 3. |
- Johansen, Kari, et al.
(author)
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Norovirus strains belonging to the GII.4 genotype dominate as a cause of nosocomial outbreaks of viral gastroenteritis in Sweden 1997-2005 - Arrival of new variants is associated with large nation-wide epidemics
- 2008
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In: Journal of Clinical Virology. - : Elsevier BV. - 1386-6532 .- 1873-5967. ; 42:2, s. 129-134
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Journal article (peer-reviewed)abstract
- Background: In recent years an increase of the incidence of nosocomial outbreaks caused by noroviruses has been observed throughout Sweden, with high peaks noted in the winter seasons 2002/2003 and 2004/2005, respectively. Objectives: To phylogenetically characterize norovirus strains causing nosocomial outbreaks from 1997 to 2005 and estimate the impact of norovirus-like disease on the Swedish health care system during the peak season 2002/2003 when a new variant of norovirus occurred. Study design: Stool samples from 115 randomly selected nosocomial outbreaks occurring during 1997-2005 throughout Sweden were studied by RT-PCR and sequencing. In addition, to investigate the impact on the health-care system, a questionnaire was distributed to infection control units (n = 90) serving all Swedish hospitals, nursing homes and other health-care institutions during the largest epidemic of nosocomial outbreaks. Results: Sequencing of 279 nucleotides of the norovirus RNA polymerase gene in stools containing norovirus RNA showed that strains belonging to the GII.4 genotype dominated. Each of the two large epidemics was due to a new variant within this cluster. The questionnaire revealed that 30,000-35,000 episodes of nosocomial norovirus-like infections occurred in 80 of 82 major Swedish hospitals affected in 2002/2003. Conclusion: New norovirus variants within the cluster GGII.4 may have a major impact on the health-care system. (c) 2008 Elsevier B.V. All rights reserved.
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| 4. |
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| 5. |
- Jonsson, Sarah, 1982-
(author)
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Pelvic inflammatory disease and epithelial ovarian tumors
- 2023
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Doctoral thesis (other academic/artistic)abstract
- Background: Epithelial ovarian cancer and borderline ovarian tumors consist of several histotypes in which high-grade serous carcinoma is the most common. The majority of epithelial ovarian tumors are considered to originate in the fimbriated end of the fallopian tubes. What initiates these tumors is far from completely understood. Pelvic inflammatory disease has been proposed as a modifiable risk factor for epithelial ovarian tumors. A major cause of pelvic inflammatory disease is Chlamydia trachomatis which has been shown to have cancer-causing potential. The overall purpose of this thesis was to study associations of pelvic inflammatory disease and C. trachomatis with risk of epithelial ovarian tumors.Methods: In a cross-sectional study (Paper I) we collected ovarian tissue and corresponding blood samples from 69 women undergoing surgery due to suspected ovarian pathology. C. trachomatis specific protein (immunohistochemistry) and C. trachomatis DNA (qPCR) in ovarian tissue were analyzed (Paper I). In a nested case-control study (Paper II) prospective blood samples from 92 women diagnosed with high-grade serous ovarian cancer were matched to four controls each for age and date of plasma sampling. C. trachomatis specific plasma antibodies were analyzed by commercial Enzyme-Linked ImmunoSorbent Assay (ELISA) and Micro-ImmunoFluorescence (MIF) (Paper I and Paper II). We performed a nationwide register-based case-control study where we included 15 072 women diagnosed with epithelial ovarian cancer (Paper III), 4782 women diagnosed with borderline ovarian tumors (Paper IV), and ten controls each matched for age and residential district. Using national Swedish registers, we retrieved data on historyof pelvic inflammatory disease and the potential confounding factors parity, educational level, previous gynecological surgery, and hormonal therapy.Results: We found C. trachomatis DNA in ovarian tissue of eight women with ovarian carcinoma, but not in ovarian tissue from women with borderline ovarian tumors or benign disease (Paper I). The prevalence of the C. trachomatis specific protein did not differ in benign and malignant tissue (Paper I). Prevalence of C. trachomatis specific plasma antibodies was similar in cases and controls at diagnosis (Paper I) and prospectively (Paper II). A history of clinically verified pelvic inflammatory disease was associated with an increased risk of epithelial ovarian cancer overall (Paper III) and borderline ovarian tumors overall (Paper IV). Histotype-specific analyses showed an increased risk of serous carcinoma (Paper III), high-grade serous carcinoma (Paper III), clear cell carcinoma (Paper III), and serous borderline ovarian tumors (Paper IV) but not significantly with other histotypes. A dose-response relationship was seen between an increased number of pelvic inflammatory disease episodes and epithelial ovarian cancer (Paper III), as well as borderline ovarian tumors (Paper IV).Conclusions: This thesis contributes to an improved understanding of the association between pelvic inflammatory disease and epithelial ovarian tumors. The results regarding C. trachomatis are inconclusive and suggests that the association of pelvic inflammatory disease with epithelial ovarian tumors acts through mechanisms other than Chlamydia alone.
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| 6. |
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| 7. |
- Jonsson, Sarah, et al.
(author)
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Pelvic inflammatory disease and risk of epithelial ovarian cancer : a national population-based case-control study in Sweden
- 2024
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In: American Journal of Obstetrics and Gynecology. - : Elsevier. - 0002-9378 .- 1097-6868. ; 230:1, s. 75.e1-75.e15
-
Journal article (peer-reviewed)abstract
- Background: Epithelial ovarian cancer is an insidious disease, and women are often diagnosed when the disease is beyond curative treatment. Accordingly, identifying modifiable risk factors is of paramount importance. Inflammation predisposes an individual to cancer in various organs, but whether pelvic inflammatory disease is associated with an increased risk of epithelial ovarian cancer has not been fully determined.Objective: This study aimed to investigate a possible association between clinically verified pelvic inflammatory disease and the risk of epithelial ovarian cancer.Study Design: In this national population-based case-control study, all women in Sweden diagnosed with epithelial ovarian cancer between 1999 and 2020 and 10 controls for each were identified, matched for age and residential district. Using several Swedish nationwide registers, data on previous pelvic inflammatory disease and potential confounding factors (age, parity, educational level, and previous gynecologic surgery) were retrieved. Adjusted odds ratios and 95% confidence intervals were estimated using conditional logistic regression. Histotype-specific analyses were performed for the subgroup of women diagnosed with epithelial ovarian cancer between 2015 and 2020. Moreover, hormonal contraceptives and menopausal hormone therapy were adjusted in addition to the aforementioned confounders.Results: This study included 15,072 women with epithelial ovarian cancer and 141,322 controls. Most women (9102 [60.4%]) had serous carcinoma. In a subgroup of cases diagnosed between 2015 and 2020, high-grade serous carcinoma (2319 [60.0%]) was identified. A total of 168 cases (1.1%) and 1270 controls (0.9%) were diagnosed with pelvic inflammatory disease. Previous pelvic inflammatory disease was associated with an increased risk of epithelial ovarian cancer (adjusted odds ratio, 1.39; 95% confidence interval, 1.17–1.66) and serous carcinoma (adjusted odds ratio, 1.46; 95% confidence interval, 1.18–1.80) for the entire study population. For the subgroup of women diagnosed in 2015–2020, pelvic inflammatory disease was associated with high-grade serous carcinoma (adjusted odds ratio, 1.43; 95% confidence interval, 1.01–2.04). The odds ratios of the other histotypes were as follows: endometrioid (adjusted odds ratio, 0.13; 95% confidence interval, 0.02–1.06), mucinous (adjusted odds ratio, 1.55; 95% confidence interval, 0.56–4.29), and clear cell carcinoma (adjusted odds ratio, 2.30; 95% confidence interval, 0.90–5.86). A dose-response relationship was observed between the number of pelvic inflammatory disease episodes and the risk of epithelial ovarian cancer (Ptrend<.001).Conclusion: A history of pelvic inflammatory disease is associated with an increased risk of epithelial ovarian cancer and a dose-response relationship is evident. Histotype-specific analyses show an association with increased risk of serous epithelial ovarian cancer and high-grade serous carcinoma and potentially also with clear cell carcinoma, but there is no significant association with other histotypes. Infection and inflammation of the upper reproductive tract might have serious long-term consequences, including epithelial ovarian cancer.
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| 8. |
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| 9. |
- Salomonsson, S., et al.
(author)
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A Population-based Investigation of the Autoantibody Profile in Mothers of Children with Atrioventricular Block
- 2011
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In: Scandinavian Journal of Immunology. - Oxford : Blackwell Publishing. - 0300-9475 .- 1365-3083. ; 74:5, s. 511-517
-
Journal article (peer-reviewed)abstract
- The objective of the study was to investigate the antigen specificity and occurrence of individual autoantibodies in mothers of children diagnosed with atrioventricular (AV) block in a nation-wide setting. Patients with AV block detected before 15 years of age were identified using national quality registries as well as a network of pediatric and adult cardiologists and rheumatologists at the six university hospitals in Sweden. Patients with gross heart malformations, surgically or infectiously induced blocks were excluded. Blood samples were obtained from the mothers and maternal autoantibody profile, including the occurrence of antibodies against Ro52, Ro60, La, SmB, SmD, RNP-70k, RNP-A, RNP-C, CENP-C, Scl-70, Jo-1, ribosomal RNP and histones was investigated in 193 mothers of children with AV block by immunoblotting and ELISA. Autoantibody reactivity was detected in 48% (93/193) of the mothers of children with AV block. In autoantibody-positive mothers, the vast majority, 95% (88/93), had antibodies against Ro52, while 63% (59/93) had autoantibodies to Ro60 and 58% (54/93) had autoantibodies to La. In addition, 13% (12/93) of the autoantibody-positive mothers had antibodies to other investigated antigens besides Ro52, Ro60 and La, and of these anti-histone antibodies were most commonly represented, detected in 8% (7/93) of the mothers. In conclusion, this Swedish population-based study confirms that maternal autoantibodies may associate with heart block in the child. Further, our data demonstrate a dominant role of Ro52 antibodies in association with AV block.
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| 10. |
- Stacey, Simon N, et al.
(author)
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A germline variant in the TP53 polyadenylation signal confers cancer susceptibility.
- 2011
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In: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 43:11, s. 1098-103
-
Journal article (peer-reviewed)abstract
- To identify new risk variants for cutaneous basal cell carcinoma, we performed a genome-wide association study of 16 million SNPs identified through whole-genome sequencing of 457 Icelanders. We imputed genotypes for 41,675 Illumina SNP chip-typed Icelanders and their relatives. In the discovery phase, the strongest signal came from rs78378222[C] (odds ratio (OR) = 2.36, P = 5.2 × 10(-17)), which has a frequency of 0.0192 in the Icelandic population. We then confirmed this association in non-Icelandic samples (OR = 1.75, P = 0.0060; overall OR = 2.16, P = 2.2 × 10(-20)). rs78378222 is in the 3' untranslated region of TP53 and changes the AATAAA polyadenylation signal to AATACA, resulting in impaired 3'-end processing of TP53 mRNA. Investigation of other tumor types identified associations of this SNP with prostate cancer (OR = 1.44, P = 2.4 × 10(-6)), glioma (OR = 2.35, P = 1.0 × 10(-5)) and colorectal adenoma (OR = 1.39, P = 1.6 × 10(-4)). However, we observed no effect for breast cancer, a common Li-Fraumeni syndrome tumor (OR = 1.06, P = 0.57, 95% confidence interval 0.88-1.27).
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| 11. |
- Stattin, Eva-Lena, et al.
(author)
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Founder mutations characterise the mutation panorama in 200 Swedish index cases referred for Long QT syndrome genetic testing
- 2012
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In: BMC Cardiovascular Disorders. - : BioMed Central. - 1471-2261 .- 1471-2261. ; 12, s. 95-
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Journal article (peer-reviewed)abstract
- Background: Long QT syndrome (LQTS) is an inherited arrhythmic disorder characterised by prolongation of the QT interval on ECG, presence of syncope and sudden death. The symptoms in LQTS patients are highly variable, and genotype influences the clinical course. This study aims to report the spectrum of LQTS mutations in a Swedish cohort.Methods: Between March 2006 and October 2009, two hundred, unrelated index cases were referred to the Department of Clinical Genetics, Umea University Hospital, Sweden, for LQTS genetic testing. We scanned five of the LQTS-susceptibility genes (KCNQ1, KCNH2, SCN5A, KCNE1, and KCNE2) for mutations by DHPLC and/or sequencing. We applied MLPA to detect large deletions or duplications in the KCNQ1, KCNH2, SCN5A, KCNE1, and KCNE2 genes. Furthermore, the gene RYR2 was screened in 36 selected LQTS genotype-negative patients to detect cases with the clinically overlapping disease catecholaminergic polymorphic ventricular tachycardia (CPVT).Results: In total, a disease-causing mutation was identified in 103 of the 200 (52%) index cases. Of these, altered exon copy numbers in the KCNH2 gene accounted for 2% of the mutations, whereas a RYR2 mutation accounted for 3% of the mutations. The genotype-positive cases stemmed from 64 distinct mutations, of which 28% were novel to this cohort. The majority of the distinct mutations were found in a single case (80%), whereas 20% of the mutations were observed more than once. Two founder mutations, KCNQ1 p.Y111C and KCNQ1 p.R518*, accounted for 25% of the genotype-positive index cases. Genetic cascade screening of 481 relatives to the 103 index cases with an identified mutation revealed 41% mutation carriers who were at risk of cardiac events such as syncope or sudden unexpected death.Conclusion: In this cohort of Swedish index cases with suspected LQTS, a disease-causing mutation was identified in 52% of the referred patients. Copy number variations explained 2% of the mutations and 3 of 36 selected cases (8%) harboured a mutation in the RYR2 gene. The mutation panorama is characterised by founder mutations (25%), even so, this cohort increases the amount of known LQTS-associated mutations, as approximately one-third (28%) of the detected mutations were unique.
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| 12. |
- Albert, Malin, et al.
(author)
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Hospitalized patients’ attitudes towards participating in a randomized control trial in case of a cardiac arrest
- 2024
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In: Resuscitation Plus. - 2666-5204. ; 18
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Journal article (peer-reviewed)abstract
- BackgroundNo previous study has evaluated patients attitudes towards inclusion in an ongoing cardiac arrest clinical trial. The aim of this study was to assess patientś willingness and motives to participate in the ongoing randomized controlled drug trial “Vasopressin and Steroids in addition to Adrenaline in cardiac arrest” (VAST-A trial) in case of an in-hospital cardiac arrest (IHCA).ObjectivesHospitalized patients, men ≥ 18 and women ≥ 50 years, were asked for informed consent for inclusion in the VAST-A trial in case of an IHCA, the reason for approving or declining inclusion in the trial and baseline characteristics.MethodsPatients admitted to hospital were asked to give informed consent of inclusion in VAST-A in case of an IHCA during their hospital stay. Patients were also asked why they approved or declined inclusion as well as baseline characteristics questions.Results1,064 patients were asked about willingness to participate in the VAST-A trial, of these 902 (84.8%) patients approved inclusion. A subgroup of 411 patients were, except willingness, also asked about motives to participate or not and basic characteristics. The main reason for approving inclusion was to contribute to research (n = 328, 83.9%). The main reason for declining inclusion was concerns regarding testing the drug treatment (n = 6, 30%).ConclusionAmong hospitalized patients the vast majority gave informed consent to inclusion in an ongoing randomized cardiac arrest drug trial. The main reason for approving inclusion was to contribute to research.
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| 13. |
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| 14. |
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| 15. |
- Amberntsson, Annika, 1974, et al.
(author)
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Investigations of sulphur deactivation of NOx storage catalysts: influence of sulphur carrier and exposure conditions
- 2002
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In: Catalysis Today. - 0920-5861. ; 73:3-4, s. 279-286
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Journal article (peer-reviewed)abstract
- The influence of SO2, H2S and COS in low concentrations on the deactivation of PURh/BaO/Al2O3 NOx storage catalysts was investigated. Different samples of the catalyst were exposed to synthetic gas mixtures mimicking lean/rich engine cycling in a mixed lean application at 400degreesC. The lean gas mixture contained 8 vol.% O2, 500 vol-ppm C3H6 and 400 vol-ppm NO balanced to 100 vol.% with Ar. The rich excursions were performed by switching off the oxygen supply. Sulphur, 25 vol-ppm of either SO2, H2S or COS, was added to the gas flow either during the lean, the rich or both periods. This procedure aimed at investigating the influence of the exposure conditions and therefore the lean and rich periods were kept equally long (5 min). In addition, thermodynamical calculations for the prevailing conditions were performed.
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| 16. |
- Andersson, Stefan, et al.
(author)
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Metod- och kvalitetsbeskrivning för geografiskt fördelade emissioner till luft under 2017
- 2017
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Reports (other academic/artistic)abstract
- Sverige rapporterar årligen nationella utsläpp till luft till UNFCCC (FN:s klimatkonvention) och CLRTAP (UNECE:s konvention om gränsöverskridande luftföroreningar), så kallade submissioner. Förutom emissioner på nationell nivå finns även behov av data med högre geografisk upplösning. För regional uppföljning av miljömålen behövs emissioner på kommun- och länsnivå.Detta dokument utgör en metod- och kvalitetsbeskrivning av geografiskt fördelade emissioner för åren 1990, 2000, 2005 samt 2010-2015. Emissionerna presenteras i 45 olika sektorer uppdelade på åtta huvudsektorer. Huvudsektorerna är El och uppvärmning, Industri (energi och processer), Transporter, Produktanvändning, Avfall och avlopp, Internationell luftfart och sjöfart, Jordbruk samt Arbetsmaskiner. Uppdelningen är förändrad jämfört med föregående år vad gäller industrins utsläpp samt utsläppen från energiförförsörjning, se avsnittet Förändringar i nationella totalemissioner samt sektorsindelning, jämfört med föregående år. De ämnen som ingår är växthusgaser, metaller, partiklar och övriga ämnen, totalt 29 ämnen.Arbetet med geografisk fördelning av Sveriges utsläpp till luft är sedan 2007 ett årligt projekt. Projektet har ett långsiktigt perspektiv med målsättningen att stegvis förbättra kvaliteten på geografiskt upplösta emissionsdata.
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| 17. |
- Andersson, Stefan, et al.
(author)
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Metod- och kvalitetsbeskrivning för geografiskt fördelade emissioner till luft (submission 2018)
- 2018
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Reports (other academic/artistic)abstract
- Sverige rapporterar årligen nationella utsläpp till luft till UNFCCC (FN:s klimatkonvention) och CLRTAP (UNECE:s konvention om gränsöverskridande luftföroreningar), så kallade submissioner. Förutom emissioner på nationell nivå finns även behov av data med högre geografisk upplösning. För regional uppföljning av miljömålen behövs emissioner på kommun- och länsnivå. Detta dokument utgör en metod- och kvalitetsbeskrivning av geografiskt fördelade emissioner för åren 1990, 2000, 2005 samt 2010-2016, rapporterade i submission 2018.Emissionerna presenteras i 55 olika sektorer uppdelade på nio huvudsektorer. Huvudsektorerna är El och fjärrvärme, Egen uppvärmning av bostäder och lokaler, Industri (energi och processer), Transporter, Arbetsmaskiner, Produktanvändning (inkl. lösningsmedel), Jordbruk, Avfall (inkl. avlopp) samt Utrikes transporter. Uppdelningen är förändrad jämfört med föregående år. För huvudsektorn Utrikes transporter fördelas eller redovisas inga växthusgaser geografiskt.Den geografiska fördelningen utförs huvudsakligen enligt konceptet ”topdown”. Detta innebär att emissioner bryts ner från en nationell totalemission för att uppnå en högre rumslig upplösning på lokal nivå. Nedbrytningen till högre rumslig upplösning kräver en geografisk begränsning av emissionerna och statistik på regional nivå.Metoden för geografisk fördelning tillåter för vissa utsläppskällor en hög rumslig upplösning (t.ex. för vägtrafik och industriprocesser). För flera sektorer är emellertid resultaten otillförlitliga om de ska studeras med högre upplösning än kommunnivå (i vissa fall även länsnivå). Resultaten från den geografiska fördelningen lagras i årsvisa emissionsdatabaser i SMHIs tekniska system för luftvårdsarbete; Airviro. Ur Airviro exporteras emissionerna till Excel-tabeller på läns- och kommunnivå. Emissionerna presenteras även på karta, samt i diagram. Publicering av resultaten sker via www.rus.lst.se. En presentation riktad mot allmänheten ges även på http://utslappisiffror.naturvardsverket.se/.Arbetet med geografisk fördelning av Sveriges utsläpp till luft är sedan 2007 ett årligt projekt. Projektet har ett långsiktigt perspektiv med målsättningen att stegvis förbättra kvaliteten på geografiskt upplösta emissionsdata. Resultaten för alla sektorer presenteras med samma geografiska upplösning även om kvaliteten varierar. På grund av detta krävs det att användare av dessa emissionsdata går igenom kvalitetsbeskrivningen och bedömer om osäkerheterna är acceptabla för den aktuella tillämpningen. Genom retroaktiva omräkningar säkerställs att metodförändringar inte orsakar trendbrott. I vissa fall har dock tillgängliga grunddata (t.ex. statistik) förändrats, vilket kan leda till icke-reella trendbrott.
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| 18. |
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| 19. |
- Bergström, Jenny, 1979-, et al.
(author)
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"English is not really a subject" : language ideologies and language learning in an introduction program
- 2024
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In: TESOL quarterly (Print). - : John Wiley & Sons. - 0039-8322 .- 1545-7249.
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Journal article (peer-reviewed)abstract
- This paper explores the construction of language ideologies and language learning within English-language education in a Language Introduction Program (LIP) in Sweden. LIP is an individual transitional program for newly arrived migrant students that seeks to quickly transition adolescents into further education or the job market. High proficiency in English is important in Swedish society and insufficient knowledge of English might lead to consequences for individuals, but also long-term consequences for society regarding inequality and inequity. The methodology is based on linguistic ethnography, with observations and interviews at two schools. Data from interviews with English teachers and principals at LIP are analyzed using Foucauldian perspectives to discuss power and individualization. Our results show that LIP is organized in a manner that reduces teachers' possibilities to cooperate and assist each other in planning, assessing, and in developing teaching practices. To a certain extent, principals withdraw from their responsibility and place a significant amount of organizational responsibility on individual English teachers. Furthermore, monolingual ideologies are prominent in educational practices, and LIP is often positioned as different from the rest of the school which increases isolation.
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| 20. |
- Björn (Fredriksson), Annika, 1972-, et al.
(author)
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Interimreport 1 for the project on natural formation, degradation and occurrence of methyltins in different habitats. : Common project between Rohm and Haas, former Morton Plastics Additive (MPA), Cincinnati, Ohio, USA and the Department of Water and Environmental Studies (DWES), Linköping Univeristy, Sweden
- 1998
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Reports (other academic/artistic)
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| 21. |
- Björn (Fredriksson), Annika, 1972-, et al.
(author)
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Interimreport 2 for the project on natural formation, degradation and occurrence of methyltins in different habitats. : Common project betweenRohm and Haas, former Morton Plastics Additive (MPA), Cincinnati, Ohio, USA and the Department of Water and Environmental Studies (DWES), Linköping Univeristy, Sweden.
- 2000
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Reports (other academic/artistic)
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| 22. |
- Boström, Annika, et al.
(author)
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Repeat surgery without preoperative angiography in limbs with patent infrainguinal bypass grafts
- 2002
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In: European Journal of Vascular and Endovascular Surgery. - : SAGE Publications. - 1078-5884 .- 1532-2165. ; 36:5, s. 343-350
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Journal article (peer-reviewed)abstract
- The aim of this study was to assess the feasibility and results of repeat surgery without preoperative angiography in limbs with patent infrainguinal bypass grafts. Between January 1995 and December 1999, 73 surgical interventions were performed for correction of inflow, graft, or runoff-related lesions in limbs with patent infrainguinal bypass grafts. Fifty-six of the 73 cases were operated on based on the findings obtained from duplex scanning alone. There were 53 vein and 3 prosthetic grafts in the series. The indications for intervention without angiography were stenotic or occlusive lesions in 35, graft aneurysm in 7, and arteriovenous fistulae in 14. There were no deviations from the preoperatively planned surgical strategy in patients undergoing surgery without preoperative angiography. Cumulative life table primary, (stenosis free) and primary-assisted patency rates, at 12 months following graft revisions (excluding arteriovenous fistulae ligatures) without preoperative angiography, were 64% and 85%, respectively. The corresponding figures for revisions performed with preoperative angiography were 58% and 84%, respectively. There were no significant differences between patients undergoing surgery with or without preoperative diagnostic angiography with regard to patency rates. Surgical interventions for correction of infrainguinal graft-related stenotic or aneurysmal lesions can be safely performed based on findings obtained from duplex scanning.
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| 23. |
- Brorsson, Ann-Christin, et al.
(author)
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The “Two-State folder” MerP forms partially unfolded structures that show temperature dependent hydrogen exchange
- 2004
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In: Journal of Molecular Biology. - London : Academic Press. - 0022-2836 .- 1089-8638. ; 340:2, s. 333-344
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Journal article (peer-reviewed)abstract
- We have analysed the folding energy landscape of the 72 amino acid protein MerP by monitoring native state hydrogen exchange as a function of temperature in the range of 7-55 degrees C. The temperature dependence of the hydrogen exchange has allowed us to determine DeltaG, DeltaH and DeltaC(p) values for the conformational processes that permit hydrogen exchange. When studied with the traditional probes, fluorescence and CD, MerP appears to behave as a typical two-state protein, but the results from the hydrogen exchange analysis reveal a much more complex energy landscape. Analysis at the individual amino acid level show that exchange is allowed from an ensemble of partially unfolded structures (i.e. intermediates) in which the stabilities at the amino acid level form a broad distribution throughout the protein. The formation of partially unfolded structures might contribute to the unusually slow folding of MerP.
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| 24. |
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| 25. |
- Cederlund, Ella, et al.
(author)
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Characterization of new medium-chain alcohol dehydrogenases adds resolution to duplications of the class I/III and the sub-class I genes
- 2011
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In: Chemico-Biological Interactions. - : Elsevier Science B.V., Amsterdam.. - 0009-2797 .- 1872-7786. ; 191:03-jan
-
Journal article (peer-reviewed)abstract
- Four additional variants of alcohol and aldehyde dehydrogenases have been purified and functionally characterized, and their primary structures have been determined. The results allow conclusions about the structural and evolutionary relationships within the large family of MDR alcohol dehydrogenases from characterizations of the pigeon (Columba livia) and dogfish (Scyliorhinus canicula) major liver alcohol dehydrogenases. The pigeon enzyme turns out to be of class I type and the dogfish enzyme of class III type. This result gives a third type of evidence, based on purifications and enzyme characterization in lower vertebrates, that the classical liver alcohol dehydrogenase originated by a gene duplication early in the evolution of vertebrates. It is discernable as the major liver form at about the level in-between cartilaginous and osseous fish. The results also show early divergence within the avian orders. Structures were determined by Edman degradations, making it appropriate to acknowledge the methodological contributions of Pehr Edman during the 65 years since his thesis at Karolinska Institutet, where also the present analyses were performed.
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| 26. |
- Chernyshev, Alexander N., et al.
(author)
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Solubilization of Ni(II) and Eu(III) through complexation with a polyaryl ether based superplasticizer in alkaline media
- 2021
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In: Chemosphere. - : Elsevier BV. - 0045-6535 .- 1879-1298. ; 263
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Journal article (peer-reviewed)abstract
- Solubilisation of Ni(II) and Eu(III) by complexation with a polyaryl ether based superplasticizer (PAE SP) in alkaline solutions was studied. The solubilization was investigated in two types of artificial cement pore waters simulating different stages of cement degradation at a pH of 12.4 and 13.3, respectively. The solubility of Ni(II) and Eu(III) increased as the concentration of superplasticizer was increased from 0.04 to 0.4 wt%. When the concentration of SP was increased from 0.4 to 4%, the solubility of Eu(III) and Ni(II) increased in the pore water with a pH of 12.4, while the concentrations decreased in the pore water with a pH of 13.3. This is explained by a more rapid degradation of the superplasticizer at higher pH leading to a release of phosphate groups and thereby precipitation of Eu(III) and Ni(II) as phosphates. Based on results of the solubilisation of Ni(II) and Eu(III) by model compounds (anisole and PEG 400) and 31P NMR spectroscopy it was confirmed that the complexation of the studied metals with the PAE polymer occurs via the phosphate group of the superplasticizer.
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| 27. |
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| 28. |
-
Death Matters : Cultural Sociology of Mortal Life
- 2019
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Editorial collection (peer-reviewed)abstract
- This book investigates death as part of contemporary everyday experience and practices. Through a cultural sociological lens, it studies death as it remains constantly at the edge of our consciousness, shaping the ways in which we move through social reality. As such, Death Matters is a significant contribution to death studies, going beyond traditional parameters of the field by addressing the cultural omnipresence of death.The contributions analyses several death-related meaning-making processes, arguing that meanings emerging from culturally shared narratives, social institutions, and material conditions, are just as important as ’death practices’ in understanding the role of death in society. Drawing on the related themes of places of absence and presence, disease and bodies, and persons and non-persons, the authors explore a variety of areas of social life, from haunting to celebrity deaths, to move the notion of death from the margins of social reality to on going everyday life.
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| 29. |
- Duffy, Stephen W., et al.
(author)
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Mammography screening reduces rates of advanced and fatal breast cancers : Results in 549,091 women
- 2020
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In: Cancer. - : John Wiley & Sons. - 0008-543X .- 1097-0142. ; 126:13, s. 2971-2979
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Journal article (peer-reviewed)abstract
- Background: It is of paramount importance to evaluate the impact of participation in organized mammography service screening independently from changes in breast cancer treatment. This can be done by measuring the incidence of fatal breast cancer, which is based on the date of diagnosis and not on the date of death.Methods: Among 549,091 women, covering approximately 30% of the Swedish screening‐eligible population, the authors calculated the incidence rates of 2473 breast cancers that were fatal within 10 years after diagnosis and the incidence rates of 9737 advanced breast cancers. Data regarding each breast cancer diagnosis and the cause and date of death of each breast cancer case were gathered from national Swedish registries. Tumor characteristics were collected from regional cancer centers. Aggregated data concerning invitation and participation were provided by Sectra Medical Systems AB. Incidence rates were analyzed using Poisson regression.Results: Women who participated in mammography screening had a statistically significant 41% reduction in their risk of dying of breast cancer within 10 years (relative risk, 0.59; 95% CI, 0.51‐0.68 [P < .001]) and a 25% reduction in the rate of advanced breast cancers (relative risk, 0.75; 95% CI, 0.66‐0.84 [P < .001]).Conclusions: Substantial reductions in the incidence rate of breast cancers that were fatal within 10 years after diagnosis and in the advanced breast cancer rate were found in this contemporaneous comparison of women participating versus those not participating in screening. These benefits appeared to be independent of recent changes in treatment regimens.
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| 30. |
- Ekman, Diana, et al.
(author)
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Stratified genetic analysis reveals sex differences in MPO-ANCA-associated vasculitis
- 2023
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In: Rheumatology. - : Oxford University Press. - 1462-0324 .- 1462-0332. ; 62:9, s. 3213-3218
-
Journal article (peer-reviewed)abstract
- Objective: To identify and genetically characterize subgroups of patients with ANCA-associated vasculitides (AAV) based on sex and ANCA subtype. Methods: A previously established SNP dataset derived from DNA sequencing of 1853 genes and genotyping of 1088 Scandinavian cases with AAV and 1589 controls was stratified for sex and ANCA subtype and analysed for association with five top AAV SNPs. rs9274619, a lead variant at the HLA-DQB1/HLA-DQA2 locus previously associated with AAV positive for myeloperoxidase (MPO)-ANCA, was analysed for association with the cumulative disease involvement of ten different organ systems. Results: rs9274619 showed a significantly stronger association to MPO-ANCA-positive females than males [P = 2.0 × 10-4, OR = 2.3 (95% CI 1.5, 3.5)], whereas proteinase 3 (PR3)-ANCA-associated variants rs1042335, rs9277341 (HLA-DPB1/A1) and rs28929474 (SERPINA1) were equally associated with females and males with PR3-ANCA. In MPO-ANCA-positive cases, carriers of the rs9274619 risk allele were more prone to disease engagement of eyes [P = 0.021, OR = 11 (95% CI 2.2, 205)] but less prone to pulmonary involvement [P = 0.026, OR = 0.52 (95% CI 0.30, 0.92)]. Moreover, AAV with both MPO-ANCA and PR3-ANCA was associated with the PR3-ANCA lead SNP rs1042335 [P = 0.0015, OR = 0.091 (95% CI 0.0022, 0.55)] but not with rs9274619. Conclusions: Females and males with MPO-ANCA-positive AAV differ in genetic predisposition to disease, suggesting at least partially distinct disease mechanisms between the sexes. Double ANCA-positive AAV cases are genetically similar to PR3-ANCA-positive cases, providing clues to the clinical follow-up and treatment of these patients.
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| 31. |
- Ekman-Ordeberg, Gunvor, et al.
(author)
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Tafoxiparin, a novel drug candidate for cervical ripening and labor augmentation: results from 2 randomized, placebo-controlled studies
- 2024
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In: American Journal of Obstetrics and Gynecology. - : Elsevier. - 0002-9378 .- 1097-6868. ; 230:3
-
Research review (peer-reviewed)abstract
- Background: Slow progression of labor is a common obstetrical problem with multiple associated complications. Tafoxiparin is a depolymerized form of heparin with a molecular structure that eliminates the anticoagulant effects of heparin. We report on 2 phase II clinical studies of tafoxiparin in primiparas. Study 1 was an exploratory, first-in-pregnant-women study and study 2 was a dose-finding study. Objective: Study 1 was performed to explore the effects on labor time of subcutaneous administration of tafoxiparin before onset of labor. Study 2 was performed to test the hypothesis that intravenous treatment with tafoxiparin reduces the risk for prolonged labor after spontaneous labor onset in situations requiring oxytocin stimulation because of dystocia. Study Design: Both studies were randomized, double-blind, and placebo-controlled. Participants were healthy, nulliparous females aged 18 to 45 years with a normal singleton pregnancy and gestational age confirmed by ultrasound. The primary endpoints were time from onset of established labor (cervical dilation of 4 cm) until delivery (study 1) and time from start of study treatment infusion until delivery (study 2). In study 1, patients at 38 to 40 weeks of gestation received 60 mg tafoxiparin or placebo daily as 0.4 mL subcutaneous injections until labor onset (maximum 28 days). In study 2, patients experiencing slow progression of labor, a prolonged latent phase, or labor arrest received a placebo or 1 of 3 short-term tafoxiparin regimens (initial bolus 7, 21, or 35 mg followed by continuous infusion at 5, 15, or 25 mg/hour until delivery; maximum duration, 36 hours) in conjunction with oxytocin. Results: The number of participants randomized in study 1 was 263, and 361 were randomized in study 2. There were no statistically significant differences in the primary endpoints between those receiving tafoxiparin and those receiving the placebo in both studies. However, in study 1, the risk for having a labor time exceeding 12 hours was significantly reduced by tafoxiparin (tafoxiparin 6/114 [5%] vs placebo 18/101 [18%]; P=.0045). Post hoc analyses showed that women who underwent labor induction had a median (range) labor time of 4.44 (1.2–8.5) hours with tafoxiparin and 7.03 (1.5–14.3) hours with the placebo (P=.0041) and that co-administration of tafoxiparin potentiates the effect of oxytocin and facilitates a shorter labor time among women with a labor time exceeding 6 to 8 hours (P=.016). Among women induced into labor, tafoxiparin had a positive effect on cervical ripening in 11 of 13 cases (85%) compared with 3 of 13 participants (23%) who received the placebo (P=.004). For women requiring oxytocin because of slow progression of labor, the corresponding results were 34 of 51 participants (66%) vs 16 of 40 participants (40%) (P=.004). In study 2, tafoxiparin had no positive effects on the secondary endpoints when compared with the placebo. Except for injection-site reactions in study 1, adverse events were no more common for tafoxiparin than for the placebo among either mothers or infants. There were few serious or treatment-related adverse events. Conclusion: Subcutaneous treatment with tafoxiparin before labor onset (study 1) may be effective in reducing the labor time among women undergoing labor induction and among those requiring oxytocin for slow progression of labor. Moreover, tafoxiparin may have a positive effect on cervical ripening. Short-term, intravenous treatment with tafoxiparin as an adjunct to oxytocin in patients with labor arrest (study 2) did not affect labor time or other endpoints. Both studies suggest that tafoxiparin has a favorable safety profile in mothers and their infants.
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| 32. |
- Elvin, Johannes, et al.
(author)
-
Melanocortin 1 Receptor Agonist Protects Podocytes Through Catalase and RhoA Activation.
- 2016
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In: American journal of physiology. Renal physiology. - : American Physiological Society. - 1522-1466 .- 1931-857X. ; 310:9
-
Journal article (peer-reviewed)abstract
- Drugs containing adrenocorticotropic hormone (ACTH) have been used as therapy for patients with nephrotic syndrome. We have previously shown that ACTH and a selective agonist for the melanocortin 1 receptor (MC1R) exert beneficial actions in experimental membranous nephropathy with reduced proteinuria, reduced oxidative stress, improved glomerular morphology and function. Our hypothesis is that MC1R activation in podocytes elicits beneficial effects by promoting stress fibers and maintaining podocyte viability. To test the hypothesis, we cultured podocytes and used highly specific agonists for the MC1R. The podocytes were subjected to the nephrotic-inducing agent puromycin aminonucleoside and downstream effects of MC1R activation on podocyte survival; antioxidant defense and cytoskeleton dynamics were studied. To increase the response and enhance the intracellular signals, podocytes were transduced to overexpress MC1R. We show that puromycin promotes MC1R expression in podocytes and that activation of the MC1R promotes an increase of catalase activity and reduces oxidative stress, which results in dephosphorylation of p190RhoGAP and formation of stress fibers through RhoA. In addition, MC1R agonists protects against apoptosis. Together, these mechanisms protect the podocyte against puromycin. Our findings strongly support the hypothesis that selective MC1R activating agonists protect podocytes and may therefore be useful to treat patients with nephrotic syndromes commonly considered as podocytopathies.
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| 33. |
- Englund, Annika, et al.
(author)
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Changes in mortality and causes of death in the Swedish Down syndrome population
- 2013
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In: American Journal of Medical Genetics. Part A. - : Wiley. - 1552-4825 .- 1552-4833. ; 161A:4, s. 642-649
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Journal article (peer-reviewed)abstract
- During the past few decades age at death for individuals with Down syndrome (DS) has increased dramatically. The birth frequency of infants with DS has long been constant in Sweden. Thus, the prevalence of DS in the population is increasing. The aim of the present study was to analyze mortality and causes of death in individuals with DS during the period 19692003. All individuals with DS that died between 1969 and 2003 in Sweden, and all individuals born with DS in Sweden between 1974 and 2003 were included. Data were obtained from the Swedish Medical Birth Register, the Swedish Birth Defects Register, and the National Cause of Death Register. Median age at death has increased by 1.8 years per year. The main cause of death was pneumonia. Death from congenital heart defects decreased. Death from atherosclerosis was rare but more frequent than reported previously. Dementia was not reported in any subjects with DS before 40 years of age, but was a main or contributing cause of death in 30% of the older subjects. Except for childhood leukemia, cancer as a cause of death was rare in all age groups. Mortality in DS, particularly infant mortality, has decreased markedly during the past decades. Median age at death is increasing and is now almost 60 years. Death from cancer is rare in DS, but death from dementia is common.
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| 34. |
- Gilljam, Marita, et al.
(author)
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Lung transplantation in patients with cystic fibrosis and Mycobacterium abscessus infection
- 2010
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In: Journal of Cystic Fibrosis. - : Elsevier BV. - 1569-1993 .- 1873-5010. ; 9:4, s. 272-276
-
Journal article (peer-reviewed)abstract
- Mycobacterium abscessus lung disease is difficult to treat and has been considered a strong relative contraindication to lung transplantation. We performed double lung transplantation in three cystic fibrosis patients with ongoing, and a fourth with recent treatment for Mycobacterium abscessus lung infection. Despite prolonged antibiotic courses and adjustment of immunosuppressive therapy the first three patients developed skin infection and abscesses. At follow-up after 1, 3, 5 and 7 years respectively no patient had evidence of M abscessus infection and all had stable lung function. Lung transplantation in patients with M abscessus lung infection is feasible but may involve severe complications.
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| 35. |
- Hagblad, Jimmie, et al.
(author)
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A technique based on laser Doppler flowmetry and photoplethysmography for simultaneously monitoring blood flow at different tissue depths
- 2010
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In: Medical and Biological Engineering and Computing. - : Springer Science Business Media. - 0140-0118 .- 1741-0444. ; 48:5, s. 415-422
-
Journal article (peer-reviewed)abstract
- The aim of this study was to validate a non-invasive optical probe for simultaneous blood flow measurement at different vascular depths combining three photoplethysmography (PPG) channels and laser Doppler flowmeter (LDF). Wavelengths of the PPG were near-infrared 810 nm with source-to-detector separation of 10 and 25 mm, and green 560 nm with source-to-detector separation of 4 mm. The probe is intended for clinical studies of pressure ulcer aetiology. The probe was placed over the trapezius muscle, and depths from the skin to the trapezius muscle were measured using ultrasound and varied between 3.8 and 23 mm in the 11 subjects included. A provocation procedure inducing a local enhancement of blood flow in the trapezius muscle was used. Blood flows at rest and post-exercise were compared. It can be concluded that this probe is useful as a tool for discriminating between blood flows at different vascular tissue depths. The vascular depths reached for the different channels in this study were at least 23 mm for the near-infrared PPG channel (source-to-detector separation 25 mm), 10-15 mm for the near-infrared PPG channel (separation 10 mm), and shallower than 4 mm for both the green PPG channel (separation 4 mm) and LDF.
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| 36. |
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| 37. |
- Hagman, Henning, 1981, et al.
(author)
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Plasmon-enhanced four-wave mixing by nanoholes in thin gold films
- 2014
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In: Optics Letters. - : Optical Society of America. - 0146-9592 .- 1539-4794. ; 39:4, s. 1001-1004
-
Journal article (peer-reviewed)abstract
- Nonlinear plasmonics opens up for wavelength conversion, reduced interaction/emission volumes, and nonlinear enhancement effects at the nanoscale with many compelling nanophotonic applications foreseen. We investigate nonlinear plasmonic responses of nanoholes in thin gold films by exciting the holes individually with tightly focused laser beams, employing a degenerated pump/probe and Stokes excitation scheme. Excitation of the holes results in efficient generation of both narrowband four-wave mixing (FWM) and broadband multiphoton excited luminescence, blueshifted relative to the excitation beams. Clear enhancements were observed when matching the pump/probe wavelength with the hole plasmon resonance. These observations show that the FWM generation is locally excited by nanoholes and has a resonant behavior primarily governed by the dimensions of the individual holes.
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| 38. |
- Hammar, Kimberley, et al.
(author)
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Low Incidence of Late Ipsilateral Ischaemic Stroke After Treatment for Symptomatic Carotid Stenosis in Sweden 2008–2017 : Increased Risk in the Elderly and After Carotid Stenting
- 2022
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In: European Journal of Vascular and Endovascular Surgery. - : Elsevier. - 1078-5884 .- 1532-2165. ; 63:1, s. 24-32
-
Journal article (peer-reviewed)abstract
- Objective: Carotid stenosis is a major risk factor for stroke and surgical treatment is key in preventing recurrent ischaemic events. Previous randomised trials have demonstrated the net benefit of surgery for significant symptomatic carotid stenosis but, with present day medical treatment, there is limited evidence on the risk of late ipsilateral ischaemic stroke (IS) and its main risk factors.Method: Ipsilateral IS after the peri-operative period (≤ 30 days) was investigated in a nationwide, registry based cohort study of patients treated for symptomatic carotid stenosis in Sweden between 2008 – 2017. The Swedish National Registry for Vascular Surgery (Swedvasc) was used to establish the cohort, and the Swedish stroke registry (Riksstroke), combined with hospital records, was used to determine outcome. Stroke of any type and all cause mortality after the peri-operative period were studied as secondary outcomes. Cox regression was used to analyse associations between clinical factors and outcomes.Results: In total, 7 589 patients (mean age 72 ± 8 years, 68% men) were followed for 4.2 ± 2.6 years. Ipsilateral IS occurred in 232 patients corresponding to a yearly incidence of 0.73%. Age above 80 years compared with 65 – 79 years was associated with an increased risk of ipsilateral IS (adjusted HR 1.94, 95% CI 1.43 – 2.65). Carotid artery stenting (CAS) compared with carotid endarterectomy (CEA) was also associated with increased risk (adjusted HR 3.20, 95% CI 2.03 – 5.03). Stroke of any type occurred in 7.7% of patients, and 19.6% of patients died during the follow up period.Conclusion: The incidence of ipsilateral IS after treatment for symptomatic carotid stenosis in Sweden 2008–2017 was low, demonstrating the effectiveness and durability of surgery in a real world setting. Only age above 80 years and CAS compared with CEA were associated with increased risk of ipsilateral IS.
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| 39. |
- Henriksson, Andreas, 1980-
(author)
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Organising Intimacy : Exploring Heterosexual Singledoms at Swedish Singles Activities
- 2014
-
Doctoral thesis (other academic/artistic)abstract
- Single activities have long been places where single people can come to meet friends, build community or look for partners. The activities have relevance for studies of heterosexuality, intimacy, personal life and space. This dissertation discusses a conference, a cruise, an online site and an association for heterosexual singles in contemporary Sweden. It shows how these activities, analysed as organising people and spaces, offer participants different versions of intimacy, relationships, personal life and ultimately singledom itself. The concept non-relationality is coined to describe how people understand and enact what it means to lack a certain kind of relationship.Multi-sited ethnographic observations are combined with interviews and a survey (n=416). The chosen methods allow insight into both the heterogeneous character of the contemporary single activity scene, as well as existing tendencies to form communities. The group whose single activities are examined is deemed fairly typical of the single population at large. Nevertheless, most conclusions centre on the specific set of activities described in the book and relate them to historical examples and theory.The single activities examined can be interpreted to enact different practices entailed in a relationship without necessarily demanding commitment to a whole relationship or a specific person. In that way, the activities accommodate the inflexible personal lives that some singles report having. This challenges strict boundaries between coupledom and singledom. Such transgressive or “hetero-doxical” potential in single activities is nevertheless circumscribed by organisers’ notion that the activities provide therapeutic community in a phase before singles take the step (back) into coupledom.
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| 40. |
- Hermansson, Carina, et al.
(author)
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The (non)effect of Joint Construction in a genre-based approach to teaching writing
- 2019
-
In: The Journal of educational research (Washington, D.C.). - : Routledge. - 0022-0671 .- 1940-0675. ; 112:4, s. 483-494
-
Journal article (peer-reviewed)abstract
- This quasi-experimental intervention study examines the effect of genre-based instructional practices on 90 primary students’ narrative writing achievements and is a result of six teachers’ action to meet the educational goals of the Swedish national curriculum. Specifically, the authors examine the effects of Joint Construction, the phase in the genre pedagogical model of the Sydney School known as the Teaching and Learning Cycle, in which teachers and students work together to co-construct texts. Joint Construction has been put forward as the most powerful part of the Teaching and Learning Cycle. The authors challenge this argument, presenting findings that are inconsistent with this widely held belief. Using a pretest-posttest control group design, the study shows that the Joint Construction stage did not significantly improve the quality of students’ narrative writing or increase the text length of their writings.
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| 41. |
- Holmberg, Tora, 1967-, et al.
(author)
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Introduction : Why death matters
- 2019
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In: Death matters. - Cham, Switzerland : Palgrave Macmillan. - 9783030114848 ; , s. 1-21
-
Book chapter (peer-reviewed)
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| 42. |
- Hök, Bertil, et al.
(author)
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Unobtrusive and Highly Accurate Breath Alcohol Analysis Enabled by Improved Methodology and Technology
- 2014
-
In: Journal of Forensic Investigation. - : Avens Publishing Group. - 2330-0396. ; 2:4, s. 1-8
-
Journal article (peer-reviewed)abstract
- The study objective was to evaluate a novel method and technology for unobtrusive determination of breath alcohol in relation to current industrial accuracy standards. The methodology uses carbon dioxide as a tracer gas detected by sensor technology based on infrared spectroscopy. Part one of the investigation was to analyse the performance of hand-held prototype devices and included tests of resolution, unit-to-unit variation during calibration, response to alcohol containing gas pulses created with a wet gas simulator, and cross sensitivity to other substances. In part two of the study, 30 human participants provided 1465 breath tests in both unobtrusive and obtrusive use modes. The results of both parts of the study indicate that the prototype devices exceeded present industrial accuracy requirements. The proposed methodology and technology eliminate the previous contradiction between unobtrusiveness and high accuracy.
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| 43. |
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| 44. |
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| 45. |
- Jiye, A, et al.
(author)
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Extraction and GC/MS analysis of the human blood plasma metabolome
- 2005
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In: ANALYTICAL CHEMISTRY. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 77:24, s. 8086-94
-
Journal article (peer-reviewed)abstract
- Analysis of the entire set of low molecular weight compounds (LMC), the metabolome, could provide deeper insights into mechanisms of disease and novel markers for diagnosis. In the investigation, we developed an extraction and derivatization protocol, using experimental design theory (design of experiment), for analyzing the human blood plasma metabolome by GC/MS. The protocol was optimized by evaluating the data for more than 500 resolved peaks using multivariate statistical tools including principal component analysis and partial least-squares projections to latent structures (PLS). The performance of five organic solvents (methanol, ethanol, acetonitrile, acetone, chloroform), singly and in combination, was investigated to optimize the LMC extraction. PLS analysis demonstrated that methanol extraction was particularly efficient and highly reproducible. The extraction and derivatization conditions were also optimized. Quantitative data for 32 endogenous compounds showed good precision and linearity. In addition, the determined amounts of eight selected compounds agreed well with analyses by independent methods in accredited laboratories, and most of the compounds could be detected at absolute levels of similar to 0.1 pmol injected, corresponding to plasma concentrations between 0.1 and 1 mu M. The results suggest that the method could be usefully integrated into metabolomic studies for various purposes, e.g., for identifying biological markers related to diseases.
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| 46. |
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| 47. |
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| 48. |
- Jonsson, Annika, 1976-
(author)
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A Nice Place : The Everyday Production of Pleasure and Political Correctness at Work
- 2009
-
Doctoral thesis (other academic/artistic)abstract
- This dissertation investigates heterosexed reality as an ongoing accomplishment by the members at a workplace. Observations were carried out sporadically for two years at a museum. During this period twelve formal interviews, fifteen informal interviews, three formal group interviews and three informal group interviews were also conducted. The study rests on an ethnomethodological understanding of how reality and order is achieved by actors in interaction through the use of ethnomethods, such as common sense. Order is produced in a number of situations and it is situations, as locations of shared practices, which are primarily focused. It is concluded that the members try to, in different ways, realise the museum as a nice place. The concept of straight-framing is introduced to describe one of the pleasure procedures performed by the members in order to generate good mood, solidarity and familiarity in everyday working life. To successfully straight-frame situations, the members must utilise the heterosexual matrix and produce themselves and others as intelligibly sexed beings, belonging to either the category women or men, and as relatable to people of the other sex in couple-like and/or sexualised (explicitly or implicitly) ways. Three different forms of straight-framing are distinguished; direct, mock direct and indirect. The members also routinely realise the museum as a nice place by creating a discourse of political correctness. The easiest way to produce and use this discourse appears to be to talk about gender equality. In conversations about gender equality women and men are commonsensically turned into a standardised relational pair and this is referred to as the body count routine. While the body count routine makes the issue of gender equality intelligible for the members and enables them to come across as politically competent, it also provides them with an opportunity to organise the working units at the museum. Sex-mixed units can be placed above non-mixed in a moral hierarchy.
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| 49. |
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| 50. |
- Jonsson, Annika, et al.
(author)
-
Afterlife - ontology and the making of continuing bonds
- 2014
-
In: Book of Abstracts. Interdisciplinary conference <em>Images of Afterlife </em> October 22–24, 2014 Conference venue: Sirkkala campus, Kaivokatu 12, University of Turku.
-
Conference paper (peer-reviewed)abstract
- Afterlife-ontology and the making of continuing bonds This presentation explores the co-construction of after life-ontology by mediums and people turning to mediums, particularly ontology which facilitate for the making of continuing bonds. By extension, the questions addressed taps into larger discussions about re-enchantment, spirituality and relationships with the dead, but they are at the same time related to intimacy, meaning and intelligibility. The analysis is based on in-depth interviews as well as conversations found on sites where after life-issues are discussed. Themes examined include experiences of the deceased as still present, imagery of the afterlife in terms of space, temporality, agents and agency, and perceptions of the possibilities to interact. The overall purpose of the presentation is to show how different ontological notions and on-going negotiations about how, where and why the deceased continue to exist result in variations when it comes to the enactment of continuing bonds. Continuing bonds are usually investigated as features of everyday life, most notably in the form of experiences of the dead as somehow present, but also as constituted by various memorialization practices. These bonds may be a way of preserving life as they know it for the living, but they can also fill other existential needs. Although continuing bonds have been explored in the context of mediumship and spiritualism before, the interest here taken in ontological elements such as agency, space and interaction enable for a more thorough investigation of what sort of continuing bonds can be developed. The interest in co-construction, i.e. how notions and imageries are shaped (and challenged) collectively, also adds to the analytical scope. (PhD) Annika Jonsson, Dep. of Social and Psychological Studies, Karlstad University. annika.jonsson@kau.se(Prof.) Lars Aronsson, Dep. of human geography, media and communication, Karlstad University. lars.aronsson@kau.se
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