| 1. |
- Petkevicius, Karolis, et al.
(author)
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Biotechnological production of the European corn borer sex pheromone in the yeast Yarrowia lipolytica
- 2021
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In: Biotechnology Journal. - : Wiley. - 1860-6768 .- 1860-7314. ; 16:6
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Journal article (peer-reviewed)abstract
- The European corn borer (ECB) Ostrinia nubilalis is a widespread pest of cereals, particularly maize. Mating disruption with the sex pheromone is a potentially attractive method for managing this pest; however, chemical synthesis of pheromones requires expensive starting materials and catalysts and generates hazardous waste. The goal of this study was to develop a biotechnological method for the production of ECB sex pheromone. Our approach was to engineer the oleaginous yeast Yarrowia lipolytica to produce (Z)-11-tetradecenol (Z11-14:OH), which can then be chemically acetylated to (Z)-11-tetradecenyl acetate (Z11-14:OAc), the main pheromone component of the Z-race of O. nubilalis. First, a C14 platform strain with increased biosynthesis of myristoyl-CoA was obtained by introducing a point mutation into the α-subunit of fatty acid synthase, replacing isoleucine 1220 with phenylalanine (Fas2pI1220F). The intracellular accumulation of myristic acid increased 8.4-fold. Next, fatty acyl-CoA desaturases (FAD) and fatty acyl-CoA reductases (FAR) from nine different species of Lepidoptera were screened in the C14 platform strain, individually and in combinations. A titer of 29.2 ± 1.6 mg L-1 Z11-14:OH was reached in small-scale cultivation with an optimal combination of a FAD (Lbo_PPTQ) from Lobesia botrana and FAR (HarFAR) from Helicoverpa armigera. When the second copies of FAD and FAR genes were introduced, the titer improved 2.1-fold. The native FAS1 gene's overexpression led to a further 1.5-fold titer increase, reaching 93.9 ± 11.7 mg L-1 in small-scale cultivation. When the same engineered strain was cultivated in controlled 1 L bioreactors in fed-batch mode, 188.1 ± 13.4 mg L-1 of Z11-14:OH was obtained. Fatty alcohols were extracted from the biomass and chemically acetylated to obtain Z11-14:OAc. Electroantennogram experiments showed that males of the Z-race of O. nubilalis were responsive to biologically-derived pheromone blend. Behavioral bioassays in a wind tunnel revealed attraction of male O. nubilalis, although full precopulatory behavior was observed less often than for the chemically synthesized pheromone blend. The study paves the way for the production of ECB pheromone by fermentation.
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| 2. |
- Holkenbrink, Carina, et al.
(author)
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Production of moth sex pheromones for pest control by yeast fermentation
- 2020
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In: Metabolic Engineering. - : Elsevier BV. - 1096-7176. ; 62, s. 312-321
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Journal article (peer-reviewed)abstract
- The use of insect sex pheromones is an alternative technology for pest control in agriculture and forestry, which, in contrast to insecticides, does not have adverse effects on human health or environment and is efficient also against insecticide-resistant insect populations. Due to the high cost of chemically synthesized pheromones, mating disruption applications are currently primarily targeting higher value crops, such as fruits. Here we demonstrate a biotechnological method for the production of (Z)-hexadec-11-en-1-ol and (Z)-tetradec-9-en-1-ol, using engineered yeast cell factories. These unsaturated fatty alcohols are pheromone components or the immediate precursors of pheromone components of several economically important moth pests. Biosynthetic pathways towards several pheromones or their precursors were reconstructed in the oleaginous yeast Yarrowia lipolytica, which was further metabolically engineered for improved pheromone biosynthesis by decreasing fatty alcohol degradation and downregulating storage lipid accumulation. The sex pheromone of the cotton bollworm Helicoverpa armigera was produced by oxidation of fermented fatty alcohols into corresponding aldehydes. The resulting yeast-derived pheromone was just as efficient and specific for trapping of H. armigera male moths in cotton fields in Greece as a conventionally produced synthetic pheromone mixture. We further demonstrated the production of (Z)-tetradec-9-en-1-yl acetate, the main pheromone component of the fall armyworm Spodoptera frugiperda. Taken together our work describes a biotech platform for the production of commercially relevant titres of moth pheromones for pest control via yeast fermentation.
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| 3. |
- Konstantopoulou, Christina, et al.
(author)
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Dust depletion of metals from local to distant galaxies : II. Cosmic dust-to-metal ratio and dust composition
- 2024
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In: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 681
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Journal article (peer-reviewed)abstract
- The evolution of cosmic dust content and the cycle between metals and dust in the interstellar medium (ISM) play a fundamental role in galaxy evolution. The chemical enrichment of the Universe can be traced through the evolution of the dust-to-metal ratio (DTM) and the dust-to-gas ratio (DTG) with metallicity. The physical processes through which dust is created and eventually destroyed remain to be elucidated. We use a novel method to determine mass estimates of the DTM, DTG, and dust composition in terms of the fraction of dust mass contributed by element X ( fMX ) based on our previous measurements of the depletion of metals in different environments (the Milky Way, the Magellanic Clouds, and damped Lyman-α absorbers (DLAs) towards quasars (QSOs) and towards gamma-ray bursts (GRBs)), which were calculated from the relative abundances of metals in the ISM through absorption-line spectroscopy column densities observed mainly from VLT/UVES and X-shooter, and HST/STIS. We also derive the dust extinction from the estimated dust depletion (AV,depl) for GRB-DLAs, the Magellanic Clouds, and the Milky Way, and compare it with the AV estimated from extinction (AV,ext). We find that the DTM and DTG ratios increase with metallicity and with the dust tracer [Zn/Fe]. This suggests that grain growth in the ISM is the dominant process of dust production, at least in the metallicity range (-2 ≤ [M/H]tot . 0.5) and redshift range (0.6 < z < 6.3) that we are studying. The increasing trend in the DTM and DTG with metallicity is in good agreement with a dust production and evolution hydrodynamical model. Our data suggest that the stellar dust yield is much lower (about 1%) than the metal yield and thus that the overall amount of dust in the warm neutral medium that is produced by stars is much lower than previously estimated. The global neutral gas metallicity is decreasing over cosmic time and is traced similarly by quasar-DLAs and GRB-DLAs. We find that, overall, AV,depl is lower than AV,ext for the Milky Way and in a few lines of sight for the Magellanic Clouds, a discrepancy that is likely related to the presence of carbonaceous dust associated with dense clumps of cold neutral gas. For the other environments studied here, we find good agreement overall between the AV,ext and AV,depl.We show that the main elements ( fMX > 1%) that contribute to the dust composition, by mass, are O, Fe, Si, Mg, C, S, Ni, and Al for all the environments, with Si, Mg, and C being equivalent contributors. There are nevertheless variations in the dust composition depending on the overall amount of dust. The abundances measured at low dust regimes in quasar- and GRB-DLAs suggest the presence of pyroxene and metallic iron in dust. These results give important information on the dust and metal content of galaxies across cosmic times, from the Milky Way up to z = 6.3.
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| 4. |
- Kueny, Jay K., et al.
(author)
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Implications for the Formation of (155140) 2005 UD from a New Convex Shape Model
- 2023
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In: The Planetary Science Journal. - : Institute of Physics (IOP). - 2632-3338. ; 4:3
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Journal article (peer-reviewed)abstract
- (155140) 2005 UD has a similar orbit to (3200) Phaethon, an active asteroid in a highly eccentric orbit thought to be the source of the Geminid meteor shower. Evidence points to a genetic relationship between these two objects, but we have yet to fully understand how 2005 UD and Phaethon could have separated into this associated pair. Presented herein are new observations of 2005 UD from five observatories that were carried out during the 2018, 2019, and 2021 apparitions. We implemented light curve inversion using our new data, as well as dense and sparse archival data from epochs in 2005–2021, to better constrain the rotational period and derive a convex shape model of 2005 UD. We discuss two equally well-fitting pole solutions (λ = 116 6, β = −53 6) and (λ = 300 3, β = −55 4), the former largely in agreement with previous thermophysical analyses and the latter interesting due to its proximity to Phaethon's pole orientation. We also present a refined sidereal period of Psid = 5.234246 ± 0.000097 hr. A search for surface color heterogeneity showed no significant rotational variation. An activity search using the deepest stacked image available of 2005 UD near aphelion did not reveal a coma or tail but allowed modeling of an upper limit of 0.04–0.37 kg s−1 for dust production. We then leveraged our spin solutions to help limit the range of formation scenarios and the link to Phaethon in the context of nongravitational forces and timescales associated with the physical evolution of the system.
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