| 1. |
- Wortman, J. R., et al.
(author)
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The 2008 update of the Aspergillus nidulans genome annotation: A community effort
- 2009
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In: Fungal Genetics and Biology. - : Elsevier BV. - 1096-0937 .- 1087-1845. ; 46, s. S2-S13
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Journal article (peer-reviewed)abstract
- The identification and annotation of protein-coding genes is one of the primary goals of whole-genome sequencing projects, and the accuracy of predicting the primary protein products of gene expression is vital to the interpretation of the available data and the design of downstream functional applications. Nevertheless, the comprehensive annotation of eukaryotic genomes remains a considerable challenge. Many genomes submitted to public databases, including those of major model organisms, contain significant numbers of wrong and incomplete gene predictions. We present a community-based reannotation of the Aspergillus nidulans genome with the primary goal of increasing the number and quality of protein functional assignments through the careful review of experts in the field of fungal biology. (C) 2009 Elsevier Inc. All rights reserved.
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| 2. |
- Kovalchuk, T, et al.
(author)
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Case Reports: Emery-Dreifuss Muscular Dystrophy Presenting as a Heart Rhythm Disorders in Children
- 2021
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In: Frontiers in cardiovascular medicine. - : Frontiers Media SA. - 2297-055X. ; 8, s. 668231-
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Journal article (other academic/artistic)abstract
- Emery-Dreifuss muscular dystrophy (EDMD) is inherited muscle dystrophy often accompanied by cardiac abnormalities in the form of supraventricular arrhythmias, conduction defects and sinus node dysfunction. Cardiac phenotype typically arises years after skeletal muscle presentation, though, could be severe and life-threatening. The defined clinical manifestation with joint contractures, progressive muscle weakness and atrophy, as well as cardiac symptoms are observed by the third decade of life. Still, clinical course and sequence of muscle and cardiac signs may be variable and depends on the genotype. Cardiac abnormalities in patients with EDMD in pediatric age are not commonly seen. Here we describe five patients with different forms of EDMD (X-linked and autosomal-dominant) caused by the mutations in EMD and LMNA genes, presented with early onset of cardiac abnormalities and no prominent skeletal muscle phenotype. The predominant forms of cardiac pathology were atrial arrhythmias and conduction disturbances that progress over time. The presented cases discussed in the light of therapeutic strategy, including radiofrequency ablation and antiarrhythmic devices implantation, and the importance of thorough neurological and genetic screening in pediatric patients presenting with complex heart rhythm disorders.
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| 4. |
- Babenko, Vladislav V., et al.
(author)
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Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
- 2020
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In: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 21:1
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Journal article (peer-reviewed)abstract
- BackgroundSalivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today.ResultsWe annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis.ConclusionsHere we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches.
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| 5. |
- Pirani, A., et al.
(author)
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Phylogeny ofAcanthophyllums.l. revisited: An update on generic concept and sectional classification
- 2020
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In: Taxon. - : Wiley. - 0040-0262 .- 1996-8175. ; 69:3, s. 500-514
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Journal article (peer-reviewed)abstract
- The generic boundary of the broadly definedAcanthophyllums.l., the third-largest genus of the tribe Caryophylleae (Caryophyllaceae), has been a subject of taxonomic confusion.Acanthophyllums.l. now includes five minor genera previously recognized as independent. Among these small genera, the inclusion ofAllochrusa,Ochotonophila, andScleranthopsiswithinAcanthophyllums.l. was confirmed by previous molecular studies, while the positions ofDiaphanopteraandKuhitangiaremained uncertain. We have performed an updated molecular study ofAcanthophyllums.l. including an increased sampling of the genera and sections assigned to this group, using intron sequences of the chloroplast generps16and nuclear ribosomal internal transcribed spacer (ITS) sequences.Cyathophylla,Heterochroa, andSaponariawere chosen as outgroups for performing phylogenetic analyses using maximum likelihood and Bayesian methods. The present results suggest that, in addition to the genera mentioned above, bothDiaphanopteraandKuhitangiashould also be synonymized withinAcanthophyllum. SectionsDiaphanoptera,KuhitangiaandPseudomacrostegiaare introduced as new infrageneric taxa withinAcanthophyllum.Our results also indicate that some annual species ofSaponariaare closely related toAcanthophyllum.
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| 6. |
- Rudko, G.Yu, et al.
(author)
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Optically detected magnetic resonance study of relaxation/emission processes in the nanoparticle-polymer composite
- 2019
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In: SPQEO. - Kiev, Ukraine : Natsional'na Akademiya Nauk Ukrainy * Instytut Fizyky Napivprovidnykiv. - 1605-6582. ; 22:3, s. 310-318
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Journal article (peer-reviewed)abstract
- Two nanocomposites containing CdS nanoparticles in polymeric matrices were studied using the photoluminescence (PL) and optically detected magnetic resonance (ODMR) methods. Due to equal sizes of NPs in the composites (~5 nm) but different matrices – the oxygen-containing polymer PVA (polyvinyl alcohol) and oxygen-free polymer PEI (polyethyleneimine) – differences of nanocomposites properties are predominantly caused by different interfacial conditions. ODMR spectra have revealed five types of centers related to the PL emission – four centers involved in radiative recombination and one center related to non-radiative recombination processes. The oxygen-related interfacial center in CdS/PVA (LK1-center) and sulfur vacancy center in CdS/PEI (Vs-center) were identified.
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| 8. |
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| 9. |
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| 10. |
- Kovalchuk, A. O., et al.
(author)
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Phosphorescence of CdS nanoparticles in polymer matrix as an indication of host-guest interaction
- 2016
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In: Materials Chemistry and Physics. - : ELSEVIER SCIENCE SA. - 0254-0584 .- 1879-3312. ; 177, s. 379-383
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Journal article (peer-reviewed)abstract
- We report on the observation of the long-lasting low-temperature photoluminescence decay in the hybrid system nano-CdS/polyvinyl alcohol with a characteristic time of about 1.7 s. The origin of the phosphorescence is ascribed to the accumulation of photo-excited excitons in the traps within the polymeric matrix with subsequent transfer of the excitation to the embedded CdS nanoparticles. (C) 2016 Elsevier B.V. All rights reserved.
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| 13. |
- Rudko, G. Yu., et al.
(author)
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Effects of ultraviolet light on optical properties of colloidal CdS nanoparticles embedded in a polymer PVA matrix
- 2012
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In: Advanced Science, Engineering and Medicine. - : American Scientific Publishers. - 2164-6627. ; 4:5, s. 394-400
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Journal article (peer-reviewed)abstract
- CdS nanoparticles have been synthesized in aqueous solution using polyvinyl alcohol (PVA) as a capping reagent. The effects of exposure by ultraviolet (UV) light on optical properties of nanocomposites consisting of colloidal CdS nanoparticles and a polymer PVA matrix were studied by employing photoluminescence (PL) spectroscopy. It is shown that UV-induced changes of the photoluminescence intensity in PVA are caused by creation and healing of non-radiative recombination centers. It is also concluded that in the nanocomposites, the UV-induced changes of the PL intensity are predominantly governed by processes at the NP/PVA interface.
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| 14. |
- Rudko, Galyna Yu, et al.
(author)
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Interfacial bonding in a CdS/PVA nanocomposite: A Raman scattering study
- 2015
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In: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797 .- 1095-7103. ; 452, s. 33-37
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Journal article (peer-reviewed)abstract
- Raman spectroscopy is employed to characterize the bonding between CdS nanoparticles (NPs) and a polyvinyl alcohol (PVA) as well as structural changes in the polymeric matrix caused by incorporation of NPs. It is shown that after the formation of CdS NPs the vibrations of carbonyl groups in acetate residuals of PVA and of CO groups at the macromolecules ends disappear. Formation of NPs also leads to an increased degree of hydrogen bonding and crystallinity of the hybrid material as compared with the unloaded polymer. The observed changes are ascribed to the formation of coordinative bonds and hydrogen between the CdS nanoparticles and polymeric macromolecules. The scheme of this interfacial bonding is also proposed.
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| 15. |
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| 16. |
- Rudko, G. Yu, et al.
(author)
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Role of the host polymer matrix in light emission processes in nano-CdS/poly vinyl alcohol composite
- 2013
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In: Thin Solid Films. - : Elsevier. - 0040-6090 .- 1879-2731. ; 543, s. 11-15
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Journal article (peer-reviewed)abstract
- Participation of a polymeric media in light-emitting processes of composite nano-CdS/polyvinyl alcohol is studied by probing different absorption-emission routes via adjustment of excitation wavelengths. It is shown that the polymeric constituent of the composite contributes chiefly to the photoluminescence excitation processes via absorption and excitation transfer to the embedded CdS nanoparticles while the composite emission occurs mostly within the nanoparticles.
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| 17. |
- Salojarvi, Jarkko, et al.
(author)
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Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch
- 2017
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In: Nature Genetics. - : NATURE PUBLISHING GROUP. - 1061-4036 .- 1546-1718. ; 49:6, s. 904-912
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Journal article (peer-reviewed)abstract
- Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make birch an attractive model for forest biotechnology. We assembled and chromosomally anchored the nuclear genome of an inbred B. pendula individual. Gene duplicates from the paleohexaploid event were enriched for transcriptional regulation, whereas tandem duplicates were overrepresented by environmental responses. Population resequencing of 80 individuals showed effective population size crashes at major points of climatic upheaval. Selective sweeps were enriched among polyploid duplicates encoding key developmental and physiological triggering functions, suggesting that local adaptation has tuned the timing of and cross-talk between fundamental plant processes. Variation around the tightly-linked light response genes PHYC and FRS10 correlated with latitude and longitude and temperature, and with precipitation for PHYC. Similar associations characterized the growth-promoting cytokinin response regulator ARR1, and the wood development genes KAK and MED5A.
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