| 1. |
- Bagriy, I.D., et al.
(author)
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H2 - Ecological source of decarbonization - The path to energy independence of Ukraine
- 2023
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Book (other academic/artistic)abstract
- The research studied the pressing problem of the fuel and energy strategy of the XXI century. For the first time, there was shown the diversity of the authors' approach to theoretical and applied problems of hydrogen energy. General issues of fundamental and applied aspects of hydrogen origin were covered. The study provides the results of scientific developments on hydrogen mapping performed within the framework of scientific and practical exploratory and geo-ecological research on more than 200 hydrocarbon objects both on land (including mining fields) and in marine water areas. Prognostic and search system criteria were substantiated, with hydrogen being used for the first time as a component of the complex of methodical solutions in the search practice. The research showed the importance of solving the problem of geo-ecological processes and accidents in mine workings and wells, connected to numerous disasters. According to numerous studies of mine massives, a set of preventive measures and criteria that prevent explosive processes was provided.The presence of a unique complex of natural components of hydrogen technology, solar and wind energy resources and fresh waters of the Danube lakes and the Danube was stated.The study emphasises the decisive role of Ukraine in the competition for primacy in scientific developments. For the first time in world practice, the implementation of global projects for the extraction and production of green and white hydrogen was substantiated.The technology was tested on numerous hydrocarbon deposits, water resources and mine workings and therefore has all the prospects for the effective application in the search and extraction of hydrogen. This monograph can be useful and informative for specialists in the fields of oil and gas geology, general and regional geology, hydrology, environmental protection, as well as for the students and postgraduate students of fuel and energy, oil and gas geological and hydrological areas, as well as for domestic and foreign investors.
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| 2. |
- Bagriy, I.D., et al.
(author)
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H2 - ENVIRONMENTAL AND ENERGY CHALLENGESOF THE XXI CENTURY. GLOBAL PROJECTSWAYS OF IMPLEMENTATION
- 2023
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Book (peer-reviewed)abstract
- The research studied the pressing problem of the fuel and energy strategy of the XXI century. For the first time, there was shown the diversity of the authors' approach to theoretical and applied problems of hydrogen energy. General issues of fundamental and applied aspects of hydrogen origin were covered. The study provides the results of scientific developments on hydrogen mapping performed within the framework of scientific and practical exploratory and geo-ecological research on more than 200 hydrocarbon objects both on land (including mining fields) and in marine water areas. Prognostic and search system criteria were substantiated, with hydrogen being used for the first time as a component of the complex of methodical solutions in the search practice. The research showed the importance of solving the problem of geo-ecological processes and accidents in mine workings and wells, connected to numerous disasters. According to numerous studies of mine massives, a set of preventive measures and criteria that prevent explosive processes was provided. The presence of a unique complex of natural components of hydrogen technology, solar and wind energy resources and fresh waters of the Danube lakes and the Danube was stated. The study emphasises the decisive role of Ukraine in the competition for primacy in scientific developments. For the first time in world practice, the implementation of global projects for the extraction and production of green and white hydrogen was substantiated. The technology was tested on numerous hydrocarbon deposits, water resources and mine workings and therefore has all the prospects for the effective application in the search and extraction of hydrogen. This monograph can be useful and informative for specialists in the fields of oil and gas geology, general and regional geology, hydrology, environmental protection, as well as for the students and postgraduate students of fuel and energy, oil and gas geological and hydrological areas, as well as for domestic and foreign investors.
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| 3. |
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| 4. |
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| 5. |
- Jansons, J, et al.
(author)
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Reciprocal Inhibition of Immunogenic Performance in Mice of Two Potent DNA Immunogens Targeting HCV-Related Liver Cancer
- 2021
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In: Microorganisms. - : MDPI AG. - 2076-2607. ; 9:5
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Journal article (peer-reviewed)abstract
- Chronic HCV infection and associated liver cancer impose a heavy burden on the healthcare system. Direct acting antivirals eliminate HCV, unless it is drug resistant, and partially reverse liver disease, but they cannot cure HCV-related cancer. A possible remedy could be a multi-component immunotherapeutic vaccine targeting both HCV-infected and malignant cells, but also those not infected with HCV. To meet this need we developed a two-component DNA vaccine based on the highly conserved core protein of HCV to target HCV-infected cells, and a renowned tumor-associated antigen telomerase reverse transcriptase (TERT) based on the rat TERT, to target malignant cells. Their synthetic genes were expression-optimized, and HCV core was truncated after aa 152 (Core152opt) to delete the domain interfering with immunogenicity. Core152opt and TERT DNA were highly immunogenic in BALB/c mice, inducing IFN-γ/IL-2/TNF-α response of CD4+ and CD8+ T cells. Additionally, DNA-immunization with TERT enhanced cellular immune response against luciferase encoded by a co-delivered plasmid (Luc DNA). However, DNA-immunization with Core152opt and TERT mix resulted in abrogation of immune response against both components. A loss of bioluminescence signal after co-delivery of TERT and Luc DNA into mice indicated that TERT affects the in vivo expression of luciferase directed by the immediate early cytomegalovirus and interferon-β promoters. Panel of mutant TERT variants was created and tested for their expression effects. TERT with deleted N-terminal nucleoli localization signal and mutations abrogating telomerase activity still suppressed the IFN-β driven Luc expression, while the inactivated reverse transcriptase domain of TERT and its analogue, enzymatically active HIV-1 reverse transcriptase, exerted only weak suppressive effects, implying that suppression relied on the presence of the full-length/nearly full-length TERT, but not its enzymatic activity. The effect(s) could be due to interference of the ectopically expressed xenogeneic rat TERT with biogenesis of mRNA, ribosomes and protein translation in murine cells, affecting the expression of immunogens. HCV core can aggravate this effect, leading to early apoptosis of co-expressing cells, preventing the induction of immune response.
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| 6. |
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| 7. |
- Ardizzone, I., et al.
(author)
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Optical properties of LaNi O3 films tuned from compressive to tensile strain
- 2020
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In: Physical Review B. - : American Physical Society. - 2469-9969 .- 2469-9950. ; 102:15
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Journal article (peer-reviewed)abstract
- Materials with strong electronic correlations host remarkable - and technologically relevant - phenomena such as magnetism, superconductivity, and metal-insulator transitions. Harnessing and controlling these effects is a major challenge, on which key advances are being made through lattice and strain engineering in thin films and heterostructures, leveraging the complex interplay between electronic and structural degrees of freedom. Here we show that the electronic structure of LaNiO3 can be tuned by means of lattice engineering. We use different substrates to induce compressive and tensile biaxial epitaxial strain in LaNiO3 thin films. Our measurements reveal systematic changes of the optical spectrum as a function of strain and, notably, an increase of the low-frequency free carrier weight as tensile strain is applied. Using density functional theory (DFT) calculations, we show that this apparently counterintuitive effect is due to a change of orientation of the oxygen octahedra. The calculations also reveal drastic changes of the electronic structure under strain, associated with a Fermi surface Lifshitz transition. We provide an online applet to explore these effects. The experimental value of integrated spectral weight below 2 eV is significantly (up to a factor of 3) smaller than the DFT results, indicating a transfer of spectral weight from the infrared to energies above 2 eV. The suppression of the free carrier weight and the transfer of spectral weight to high energies together indicate a correlation-induced band narrowing and free carrier mass enhancement due to electronic correlations. Our findings provide a promising avenue for the tuning and control of quantum materials employing lattice engineering.
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| 8. |
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| 9. |
- Latanova, A. A., et al.
(author)
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Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity
- 2018
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In: Scientific Reports. - : NATURE PUBLISHING GROUP. - 2045-2322. ; 8
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Journal article (peer-reviewed)abstract
- DNA vaccines require a considerable enhancement of immunogenicity. Here, we optimized a prototype DNA vaccine against drug-resistant HIV-1 based on a weak Th2-immunogen, HIV-1 reverse transcriptase (RT). We designed expression-optimized genes encoding inactivated wild-type and drug-resistant RTs (RT-DNAs) and introduced them into mice by intradermal injections followed by electroporation. RT-DNAs were administered as single or double primes with or without cyclic-di-GMP, or as a prime followed by boost with RT-DNA mixed with a luciferase-encoding plasmid ("surrogate challenge"). Repeated primes improved cellular responses and broadened epitope specificity. Addition of cyclic-di-GMP induced a transient increase in IFN-gamma production. The strongest anti-RT immune response was achieved in a prime-boost protocol with electroporation by short 100V pulses done using penetrating electrodes. The RT-specific response, dominated by CD4+T-cells, targeted epitopes at aa 199-220 and aa 528-543. Drug-resistance mutations disrupted the epitope at aa 205-220, while the CTL epitope at aa 202-210 was not affected. Overall, multiparametric optimization of RT strengthened its Th2- performance. A rapid loss of RT/luciferase-expressing cells in the surrogate challenge experiment revealed a lytic potential of anti-RT response. Such lytic CD4+ response would be beneficial for an HIV vaccine due to its comparative insensitivity to immune escape.
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