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(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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- Aad, G, et al.
(author)
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Search for Higgs Boson Pair Production in the γγbb[over ¯] Final State Using pp Collision Data at sqrt[s]=8 TeV from the ATLAS Detector.
- 2015
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In: Physical Review Letters. - 1079-7114. ; 114:8
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Journal article (peer-reviewed)abstract
- Searches are performed for resonant and nonresonant Higgs boson pair production in the γγbb[over ¯] final state using 20 fb^{-1} of proton-proton collisions at a center-of-mass energy of 8 TeV recorded with the ATLAS detector at the CERN Large Hadron Collider. A 95% confidence level upper limit on the cross section times branching ratio of nonresonant production is set at 2.2 pb, while the expected limit is 1.0 pb. The difference derives from a modest excess of events, corresponding to 2.4 standard deviations from the background-only hypothesis. The limit observed in the search for a narrow X→hh resonance ranges between 0.7 and 3.5 pb as a function of the resonance mass.
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- Aad, G., et al.
(author)
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- 2014
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Journal article (peer-reviewed)
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| 25. |
- Aad, G., et al.
(author)
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- 2014
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In: Physical Review C (Nuclear Physics). - 0556-2813 .- 1089-490X. ; 90:4
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Journal article (peer-reviewed)
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| 26. |
- Aad, G., et al.
(author)
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- 2014
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In: The European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6052. ; 74:8
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Journal article (peer-reviewed)
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- Aad, G., et al.
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- 2014
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In: The European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6052. ; 74:6
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- Aad, G., et al.
(author)
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- 2014
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In: The European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6052. ; 74:10
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- 2014
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- 2014
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| 35. |
- Aad, G., et al.
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- 2014
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In: Physical Review C (Nuclear Physics). - 0556-2813 .- 1089-490X. ; 90:2
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- Aad, G., et al.
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| 45. |
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- 2014
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| 46. |
- Aad, G., et al.
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- 2014
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| 47. |
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- 2014
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| 48. |
- Aad, G., et al.
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- 2014
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In: The European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6052. ; 74:12
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| 49. |
- Aad, G., et al.
(author)
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- 2014
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swepub:Mat__t
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| 50. |
- Aad, G., et al.
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- 2014
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Journal article (peer-reviewed)
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