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2.
  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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3.
  • Klionsky, Daniel J., et al. (author)
  • Guidelines for the use and interpretation of assays for monitoring autophagy
  • 2012
  • In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
  • Research review (peer-reviewed)abstract
    • In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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4.
  • Hu, Mengqiang, et al. (author)
  • Comparing calculation methods of state transfer matrix in Markov chain models for indoor contaminant transport
  • 2022
  • In: Building and Environment. - : Elsevier BV. - 0360-1323 .- 1873-684X. ; 207, s. 108515-
  • Journal article (peer-reviewed)abstract
    • Fast and accurate prediction of indoor airborne contaminant distribution is of great significance to the safety and health of occupants. Several Markov chain models have been developed and proved to be the potential solutions. However, there is a lack of comparison in terms of accuracy, computing cost, and robustness among these models, which limits their practical application. To this end, this study compared the performance of three Markov chain models, in which the state transfer matrix was calculated based on different principles, i.e., Markov chain model with flux-based method, with Lagrangian tracking, and with set theory approach. The investigation was conducted in a 2D ventilated cavity and a two-zone ventilated chamber. The simulation by Eulerian model for contaminant and experimental data were used as the benchmarks for the 2D and 3D cases, respectively. It is revealed that all three Markov chain models can provide a correct prediction. In the 2D case, the Markov chain model with set theory approach is the most accurate, followed by Lagrangian tracking. In the 3D case, the accuracy of Markov chain models with flux-based method and Lagrangian tracking is comparable. The Markov chain model with Lagrangian tracking is the fastest, and the time step size in this model can be relatively large. Finally, the selection guideline is given for the application of Markov chain models in different scenarios.
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5.
  • Dai, Haolei, et al. (author)
  • Enhanced double resonance Raman scattering in multilayer graphene with broadband coherent anti-Stokes Raman spectroscopy
  • 2023
  • In: Nanoscale. - : Royal Society of Chemistry (RSC). - 2040-3364 .- 2040-3372. ; 16:3, s. 1247-1253
  • Journal article (peer-reviewed)abstract
    • Graphene's unique gapless band structure and remarkably large third-order optical susceptibility have drawn significant attention to its nonlinear optical response, particularly in the context of coherent anti-Stokes Raman scattering (CARS). Under the combined influence of phononic and electronic resonances, the CARS response of graphene has been observed to exhibit a distinctive feature of time-resolved dip-to-peak evolution. Here, we report a greatly enhanced double resonance Raman mode beyond the G mode of multi-layer graphene with broadband CARS measurements. The significant difference in the intensity ratio between CARS and SR for this mode may be attributed to the preferential activation of low-frequency phonons in the impulsive stimulated Raman scattering process (ISRS) and a lower dephasing rate. Our results build on a foundation towards a deeper exploration of the coherent Raman response of two-dimensional materials.
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6.
  • Sampson, Joshua N., et al. (author)
  • Analysis of Heritability and Shared Heritability Based on Genome-Wide Association Studies for 13 Cancer Types
  • 2015
  • In: Journal of the National Cancer Institute. - : Oxford University Press (OUP). - 0027-8874 .- 1460-2105. ; 107:12
  • Journal article (peer-reviewed)abstract
    • Background: Studies of related individuals have consistently demonstrated notable familial aggregation of cancer. We aim to estimate the heritability and genetic correlation attributable to the additive effects of common single-nucleotide polymorphisms (SNPs) for cancer at 13 anatomical sites. Methods: Between 2007 and 2014, the US National Cancer Institute has generated data from genome-wide association studies (GWAS) for 49 492 cancer case patients and 34 131 control patients. We apply novel mixed model methodology (GCTA) to this GWAS data to estimate the heritability of individual cancers, as well as the proportion of heritability attributable to cigarette smoking in smoking-related cancers, and the genetic correlation between pairs of cancers. Results: GWAS heritability was statistically significant at nearly all sites, with the estimates of array-based heritability, h(l)(2), on the liability threshold (LT) scale ranging from 0.05 to 0.38. Estimating the combined heritability of multiple smoking characteristics, we calculate that at least 24% (95% confidence interval [CI] = 14% to 37%) and 7% (95% CI = 4% to 11%) of the heritability for lung and bladder cancer, respectively, can be attributed to genetic determinants of smoking. Most pairs of cancers studied did not show evidence of strong genetic correlation. We found only four pairs of cancers with marginally statistically significant correlations, specifically kidney and testes (rho = 0.73, SE = 0.28), diffuse large B-cell lymphoma (DLBCL) and pediatric osteosarcoma (rho = 0.53, SE = 0.21), DLBCL and chronic lymphocytic leukemia (CLL) (rho = 0.51, SE = 0.18), and bladder and lung (rho = 0.35, SE = 0.14). Correlation analysis also indicates that the genetic architecture of lung cancer differs between a smoking population of European ancestry and a nonsmoking Asian population, allowing for the possibility that the genetic etiology for the same disease can vary by population and environmental exposures. Conclusion: Our results provide important insights into the genetic architecture of cancers and suggest new avenues for investigation.
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7.
  • Sun, Kwang-Hsiao, et al. (author)
  • In vivo study of alginate hydrogel conglutinating cells to polycaprolactone vascular scaffolds fabricated by electrospinning
  • 2017
  • In: Journal of Biomedical Materials Research. Part B - Applied biomaterials. - : WILEY. - 1552-4973 .- 1552-4981. ; 105:8, s. 2443-2454
  • Journal article (peer-reviewed)abstract
    • Objective The aim of this study was to explore an innovative cell-seeding technology applied on artificial vascular scaffolds. Methods Scaffolds were fabricated by electrospinning polycaprolactone (PCL) and seeded with rat endothelial progenitor cells differentiated from adipose-derived stem cells. Then, we modified the PCL scaffolds through the use of alginate hydrogel conglutinating cells (AHCC), a blank alginate hydrogel coating (BAHC), and natural sedimentation seeding cells (NSSC). The blank PCL (BP) scaffolds without any modifications were considered the blank control group. After modification, the scaffolds were implanted in a rat model. The implanted scaffolds were harvested and observed using histological and immunohistochemical methods and scanning electron microscopy (SEM) at 1, 2, and 4weeks after implantation, respectively. Results The best regeneration and configuration of the endothelium tissue and the most similar morphology to that of natural endangium was observed qualitatively in the AHCC scaffolds. The BP scaffolds had qualitatively the worst regeneration and configuration and the most dissimilar morphology at the same time point. In the AHCC group, cells could adhere directly on the inner surface of the vascular scaffolds, eliminating the time delay via the NSSC method prior to cell adhesion. Conclusion AHCC are an effective method for seeding cells on vascular scaffolds and can eliminate the time delay for cell adhesion. (C) 2016 Wiley Periodicals, Inc.
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8.
  • Chong, Hui, et al. (author)
  • Organo-ptii complexes for potent photodynamic inactivation of multi-drug resistant bacteria and the influence of configuration
  • 2024
  • In: Advanced Science. - : John Wiley & Sons. - 2198-3844. ; 11:14
  • Journal article (peer-reviewed)abstract
    • PtII based organometallic photosensitizers (PSs) have emerged as novel potent photodynamic inactivation (PDI) reagents through their enhanced intersystem crossing (ISC) processes. Currently, few PtII PSs have been investigated as antibacterial materials, with relatively poor performances reported and with structure-activity relationships not well described. Herein, a pair of configurational isomers are reported of Bis-BODIPY (4,4-difluoro-boradizaindacene) embedded PtII PSs. The cis-isomer (cis-BBP) displayed enhanced 1O2 generation and better bacterial membrane anchoring capability as compared to the trans-isomer (trans-BBP). The effective PDI concentrations (efficiency > 99.9%) for cis-BBP in Acinetobacter baumannii (multi-drug resistant (MDR)) and Staphylococcus aureus are 400 nM (12 J cm−2) and 100 nM (18 J cm−2), respectively; corresponding concentrations and light doses for trans-BBP in the two bacteria are 2.50 µM (30 J cm−2) and 1.50 µM (18 J cm−2), respectively. The 50% and 90% minimum inhibitory concentration (MIC50 and MIC90) ratio of trans-BBP to cis-BBP is 22.22 and 24.02 in A. baumannii (MDR); 21.29 and 22.36 in methicillin resistant S. aureus (MRSA), respectively. Furthermore, cis-BBP displays superior in vivo antibacterial performance, with acceptable dark and photoinduced cytotoxicity. These results demonstrate cis-BBP is a robust light-assisted antibacterial reagent at sub-micromolecular concentrations. More importantly, configuration of PtII PSs should be an important issue to be considered in further PDI reagents design.
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9.
  • Han, Jing, et al. (author)
  • Identification of N-glycoproteins of knee cartilage from adult osteoarthritis and Kashin-Beck disease based on quantitative glycoproteomics, compared with normal control cartilage
  • 2022
  • In: Cells. - : MDPI. - 2073-4409. ; 11:16, s. 2513-2513
  • Journal article (peer-reviewed)abstract
    • Glycoproteins are involved in the development of many diseases, while the type and content of N-glycoproteins in the cartilage of osteoarthritis (OA) and Kashin-Beck disease (KBD) are still unclear. This research aims to identify N-glycoproteins in knee cartilage patients with OA and KBD compared with normal control (N) adults. The cartilage samples were collected from gender- and age-matched OA (n = 9), KBD (n = 9) patients, and N (n = 9) adults. Glycoproteomics and label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) obtained N-glycoproteins of KBD and OA. A total of 594 N-glycoproteins and 1146 N-glycosylation peptides were identified. The identified data were further compared and analyzed with Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interactions (PPI). Pairwise comparison of the glycoproteins detected in the three groups showed that integrin beta-1 (ITGB1), collagen alpha-1 (II) chain (COL2A1), collagen alpha-1 (VII) chain (COL7A1), carbohydrate sulfotransferase 3 (CHST-3), carbohydrate sulfotransferase 4 (CHST-4), thrombospondin 2 (THBS2), bone morphogenetic protein 8A (BMP8A), tenascin-C (TNC), lysosome-associated membrane protein (LAMP2), and beta-glucuronidase (GUSB) were significantly differentially expressed. GO results suggested N-glycoproteins mainly belonged to protein metabolic process, single-multicellular and multicellular organism process, cell adhesion, biological adhesion, and multicellular organism development. KEGG and PPI results revealed that key N-glycoproteins were closely related to pathways for OA and KBD, such as phagosome, ECM-receptor interaction, lysosome, focal adhesion, protein digestion, and absorption. These results reflected glycoprotein expression for OA and KBD in the process of ECM degradation, material transport, cell-cell or cell-ECM interaction, and information transduction. These key significantly differentially expressed N-glycoproteins and pathways lead to the degeneration and degradation of the cartilage of OA and KBD mainly by disrupting the synthesis and catabolism of basic components of ECM and chondrocytes and interfering with the transfer of material or information. The key N-glycoproteins or pathways in this research are potential targets for pathological mechanisms and therapies of OA and KBD.
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10.
  • Liu, C, et al. (author)
  • A DNA methylation biomarker of alcohol consumption.
  • 2018
  • In: Molecular Psychiatry. - : Springer Science and Business Media LLC. - 1359-4184 .- 1476-5578. ; 23, s. 422-433
  • Journal article (peer-reviewed)abstract
    • The lack of reliable measures of alcohol intake is a major obstacle to the diagnosis and treatment of alcohol-related diseases. Epigenetic modifications such as DNA methylation may provide novel biomarkers of alcohol use. To examine this possibility, we performed an epigenome-wide association study of methylation of cytosine-phosphate-guanine dinucleotide (CpG) sites in relation to alcohol intake in 13 population-based cohorts (ntotal=13 317; 54% women; mean age across cohorts 42-76 years) using whole blood (9643 European and 2423 African ancestries) or monocyte-derived DNA (588 European, 263 African and 400 Hispanic ancestry) samples. We performed meta-analysis and variable selection in whole-blood samples of people of European ancestry (n=6926) and identified 144 CpGs that provided substantial discrimination (area under the curve=0.90-0.99) for current heavy alcohol intake (⩾42 g per day in men and ⩾28 g per day in women) in four replication cohorts. The ancestry-stratified meta-analysis in whole blood identified 328 (9643 European ancestry samples) and 165 (2423 African ancestry samples) alcohol-related CpGs at Bonferroni-adjusted P<1 × 10(-7). Analysis of the monocyte-derived DNA (n=1251) identified 62 alcohol-related CpGs at P<1 × 10(-7). In whole-blood samples of people of European ancestry, we detected differential methylation in two neurotransmitter receptor genes, the γ-Aminobutyric acid-A receptor delta and γ-aminobutyric acid B receptor subunit 1; their differential methylation was associated with expression levels of a number of genes involved in immune function. In conclusion, we have identified a robust alcohol-related DNA methylation signature and shown the potential utility of DNA methylation as a clinically useful diagnostic test to detect current heavy alcohol consumption.
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11.
  • Liu, Fangcen, et al. (author)
  • Biocompatible Nanoparticles as a Platform for Enhancing Antitumor Efficacy of Cisplatin-Tetradrine Combination
  • 2021
  • In: Nanoscale Research Letters. - : Springer. - 1931-7573 .- 1556-276X. ; 16:1
  • Journal article (peer-reviewed)abstract
    • Combination therapy has been a standard strategy in the clinical tumor treatment. We have demonstrated that combination of Tetradrine (Tet) and Cisplatin (CDDP) presented a marked synergistic anticancer activity, but inevitable side effects limit their therapeutic concentration. Considering the different physicochemical and pharmacokinetic properties of the two drugs, we loaded them into a nanovehicle together by the improved double emulsion method. The nanoparticles (NPs) were prepared from the mixture of poly(ethyleneglycol)-polycaprolactone (PEG-PCL) and polycarprolactone (HO-PCL), so CDDP and Tet can be located into the NPs simultaneously, resulting in low interfering effect and high stability. Images from fluorescence microscope revealed the cellular uptake of both hydrophilic and hydrophobic agents delivered by the NPs. In vitro studies on different tumor cell lines and tumor tissue revealed increased tumor inhibition and apoptosis rates. As to the in vivo studies, superior antitumor efficacy and reduced side effects were observed in the NPs group. Furthermore, (18)FDG-PET/CT imaging demonstrated that NPs reduced metabolic activities of tumors more prominently. Our results suggest that PEG-PCL block copolymeric NPs could be a promising carrier for combined chemotherapy with solid efficacy and minor side effects.
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12.
  • Liu, Huan, et al. (author)
  • The first human induced pluripotent stem cell line of Kashin–Beck disease reveals involvement of heparan sulfate proteoglycan biosynthesis and PPAR pathway
  • 2022
  • In: The FEBS Journal. - : John Wiley & Sons. - 1742-464X .- 1742-4658. ; 289:1, s. 279-293
  • Journal article (peer-reviewed)abstract
    • OBJECTIVE: Kashin-Beck disease (KBD) is an endemic osteochondropathy. Due to a lack of suitable animal or cellular disease models, the research progress on KBD has been limited. Our goal was to establish the first disease-specific human induced pluripotent stem cells (hiPSCs) cellular disease model of KBD, and to explore its etiology and pathogenesis exploiting transcriptome sequencing.METHODS: HiPSCs were reprogrammed from dermal fibroblasts of two KBD and one healthy control donors via integration-free vectors. Subsequently, hiPSCs were differentiated into chondrocytes through three-week culture. Gene expression profiles in KBD, normal primary chondrocytes and hiPSC-derived chondrocytes were defined by RNA sequencing. A Venn diagram was constructed to show the number of shared differentially expressed genes (DEGs) between KBD and normal. Gene oncology and Kyoto Encyclopedia of Genes and Genomes annotations were performed, and six DEGs were further validated in other individuals by real-time quantitative reverse transcription PCR (RT-qPCR).RESULTS: KBD cellular disease models were successfully established by generation of hiPSC lines. Seventeen consistent and significant DEGs present in all compared groups (KBD and normal) were identified. RT-qPCR validation gave consistent results with the sequencing data. Glycosaminoglycan biosynthesis-heparan sulfate/heparin, PPAR signaling pathway and cell adhesion molecules (CAMs) pathways were identified to be significantly altered in KBD.CONCLUSION: Differentiated chondrocytes deriving from KBD-origin hiPSCs provide the first cellular disease model for etiological studies of KBD. This study also provides new sights into the pathogenesis and etiology of KBD and is likely to inform the development of targeted therapeutics for its treatment.
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13.
  • Liu, Lizheng, et al. (author)
  • A Design of Autonomous Error-Tolerant Architectures for Massively Parallel Computing
  • 2018
  • In: IEEE Transactions on Very Large Scale Integration (vlsi) Systems. - : Institute of Electrical and Electronics Engineers (IEEE). - 1063-8210 .- 1557-9999. ; 26:10, s. 2143-2154
  • Journal article (peer-reviewed)abstract
    • The massively parallel computing systems composed of many processors are connected on chips, which will become more and more complex and unreliable. This paper presents an error-tolerant design based on the autonomous error-tolerant (AET) architecture that aims to have a self-repairing capability. A nearby error sensing mechanism is designed to discover faults, and an active evolution scheme is studied to handle unrecoverable errors. A circuit backup switching mechanism is proposed to bypass the failed nodes. The board-level prototype is implemented based on dual-core embedded processors. The analysis shows that the error-tolerant capability of the proposed architecture is better than the conventional multimodular redundant system when the failure rate of a single core is less than 0.7. In the AET test system consisting of 16 processors, the error-tolerant capability is verified. The results show that the relative variation of the overall performance of the AET system will not be changed due to the high reliability requirements of the system. Through experimental comparison, under the premise that the architecture of AET and the triple modular redundancy method are basically consistent in reliability, whether on the logical-level error tolerant or on the physical-level error tolerant, the former has lower power consumption.
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14.
  • Liu, Li, et al. (author)
  • Involvement of yes-associated protein 1 activation in the matrix degradation of human-induced-pluripotent-stem-cell-derived chondrocytes induced by T-2 toxin and deoxynivalenol alone and in combination
  • 2024
  • In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 25:2
  • Journal article (peer-reviewed)abstract
    • T-2 toxin and deoxynivalenol (DON) are two prevalent mycotoxins that cause cartilage damage in Kashin-Beck disease (KBD). Cartilage extracellular matrix (ECM) degradation in chondrocytes is a significant pathological feature of KBD. It has been shown that the Hippo pathway is involved in cartilage ECM degradation. This study aimed to examine the effect of YAP, a major regulator of the Hippo pathway, on the ECM degradation in the hiPS-derived chondrocytes (hiPS-Ch) model of KBD. The hiPS-Ch injury models were established via treatment with T-2 toxin/DON alone or in combination. We found that T-2 toxin and DON inhibited the proliferation of hiPS-Ch in a dose-dependent manner; significantly increased the levels of YAP, SOX9, and MMP13; and decreased the levels of COL2A1 and ACAN (all p values < 0.05). Immunofluorescence revealed that YAP was primarily located in the nuclei of hiPS-Ch, and its expression level increased with toxin concentrations. The inhibition of YAP resulted in the dysregulated expression of chondrogenic markers (all p values < 0.05). These findings suggest that T-2 toxin and DON may inhibit the proliferation of, and induce the ECM degradation, of hiPS-Ch mediated by YAP, providing further insight into the cellular and molecular mechanisms contributing to cartilage damage caused by toxins.
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15.
  • Liu, Minzhang, et al. (author)
  • Numerical study on aerodynamic pressure caused by high-speed train passing through the subway station
  • 2024
  • In: The International Journal of Ventilation. - : Taylor & Francis. - 1473-3315 .- 2044-4044.
  • Journal article (peer-reviewed)abstract
    • As the demand for efficient travel increased, the train speed is greatly increased and the subway gradually appeared the express train and the slow train. Express trains pass through some stations without stopping. In this case, the pressure transient phenomenon in the tunnel will become severe. In this study, the dynamic mesh simulation is used to study the pressure variations of the tunnel, platform screen door (PSD), and airshaft caused by the piston wind which is generated by the high-speed train passing through the tunnel and station. The effects of train speed, modes of the train passing through the station, number of airshafts and station types on the aerodynamic pressure are analyzed. The results demonstrate that the increase in train speed brings new challenges to the loading capacity of tunnel structure. Airshafts are set at the entrance and exit of the station, which is conducive to the pressure relief of each part of the tunnel structure. Furthermore, a new type of station with two tracks (express line and slow line) is conducted. The peak pressure of PSD is reduced by 48% compared with the conventional station. This study will contribute to the improvement of subway construction and provide theoretical and data support for the operation of the high-speed train.
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16.
  • Liu, Weiyue, et al. (author)
  • Study on moving fire smoke characteristics and mechanical ventilation system of tunnel
  • 2023
  • In: Fire safety journal. - : Elsevier. - 0379-7112 .- 1873-7226. ; 141
  • Journal article (peer-reviewed)abstract
    • Limited by the narrow space of the tunnel, fire has become one of the important factors threatening the safe operation of subway especially the fire on a moving vehicle. Researches on moving fire through the large length-width ratio of tunnels are extremely lacking at present. This study investigates the characteristics of moving fire and the influence of mechanical ventilation under different conditions. First, a three-dimensional model of the tunnel with the moving train is established, and the dynamic mesh simulation is conducted and validated by the experiments. Then, the variation of smoke temperature distribution under different fire source intensities, train speeds, and blocking ratios are studied. Based on the smoke flow characteristics, the mechanical ventilation with different layouts of draught fans is investigated. It is found that the maximum growth rate of smoke spread is 70.90% with an increase of two fans. Finally, the influence of the tunnel shaft on the smoke characteristics under different fire source locations is discussed. When the fire source is under the shaft, the smoke discharge efficiency reaches the highest, almost 5.89% and 21.91% higher than other conditions. This study provides the basis and reference for the research and control of the tunnel moving fire.
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17.
  • Lyu, Yizhen, et al. (author)
  • Identification of proteins and N-glycosylation sites of knee cartilage in Kashin-Beck Disease compared with osteoarthritis
  • 2022
  • In: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 210, s. 128-138
  • Journal article (peer-reviewed)abstract
    • The aim of this study was to identify crucial proteins and N-glycosylated sites in the pathological mechanism of Kashin-Beck Disease (KBD) compared with osteoarthritis (OA). Nine KBD knee subjects and nine OA knee subjects were selected for the study. Quantitative proteomics and N-glycoproteomics data of KBD and OA were obtained by protein and N-glycoprotein enrichment and LC-MS/MS analysis. Differentially expressed proteins or N-glycosylation sites were examined with a comparative analysis between KBD and OA. Total 2205 proteins were identified in proteomic analysis, of which 375 were significantly different. Among these, 121 proteins were up-regulated and 254 were down-regulated. In N-glycoproteomic analysis, 278 different N-glycosylated sites that were related to 187 N-glycoproteins were identified. Proteins and their N-glycosylated sites are associated with KBD pathological process including ITGB1, LRP1, ANO6, COL1A1, MXRA5, DPP4, and CSPG4. CRLF1 and GLG1 are proposed to associate with both KBD and OA pathological processes. Key pathways in KBD vs. OA proteomic and N-glycoproteomic analysis contained extracellular matrix receptor interaction, focal adhesion, phagosome, protein digestion, and absorption. N-glycosylation may influence the pathological process by affecting the integrity of chondrocytes or cartilage. It regulated the intercellular signal transduction pathway, which contributes to cartilage destruction in KBD.
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18.
  • Ma, Jiantao, et al. (author)
  • A Peripheral Blood DNA Methylation Signature of Hepatic Fat Reveals a Potential Causal Pathway for Nonalcoholic Fatty Liver Disease
  • 2019
  • In: Diabetes. - : AMER DIABETES ASSOC. - 0012-1797 .- 1939-327X. ; 68:5, s. 1073-1083
  • Journal article (peer-reviewed)abstract
    • Nonalcoholic fatty liver disease (NAFLD) is a risk factor for type 2 diabetes (T2D). We aimed to identify the peripheral blood DNA methylation signature of hepatic fat. We conducted epigenome-wide association studies of hepatic fat in 3,400 European ancestry (EA) participants and in 401 Hispanic ancestry and 724 African ancestry participants from four population-based cohort studies. Hepatic fat was measured using computed tomography or ultrasound imaging and DNA methylation was assessed at >400,000 cytosine-guanine dinucleotides (CpGs) in whole blood or CD14+ monocytes using a commercial array. We identified 22 CpGs associated with hepatic fat in EA participants at a false discovery rate <0.05 (corresponding P = 6.9 x 10(-6)) with replication at Bonferroni-corrected P < 8.6 x 10(-4). Mendelian randomization analyses supported the association of hypomethylation of cg08309687 (LINC00649) with NAFLD (P = 2.5 x 10(-4)). Hypomethylation of the same CpG was also associated with risk for new-onset T2D (P = 0.005). Our study demonstrates that a peripheral blood-derived DNA methylation signature is robustly associated with hepatic fat accumulation. The hepatic fat-associated CpGs may represent attractive biomarkers for T2D. Future studies are warranted to explore mechanisms and to examine DNA methylation signatures of NAFLD across racial/ethnic groups.
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19.
  • Pan, Xiong-Fei, et al. (author)
  • Circulating fatty acids and risk of gestational diabetes mellitus : prospective analyses in China
  • 2021
  • In: European Journal of Endocrinology. - : Bioscientifica. - 0804-4643 .- 1479-683X. ; 185:1, s. 87-97
  • Journal article (peer-reviewed)abstract
    • Objective: We aimed to examine prospective associations between circulating fatty acids in early pregnancy and incident gestational diabetes mellitus (GDM) among Chinese pregnant women.Methods: Analyses were based on two prospective nested case-control studies conducted in western China (336 GDM cases and 672 matched controls) and central China (305 cases and 305 matched controls). Fasting plasma fatty acids in early pregnancy (gestational age at enrollment: 10.4 weeks(s.d., 2.0)) and 13.2 weeks (1.0), respectively) were determined by gas chromatography-mass spectrometry, and GDM was diagnosed based on the International Association of Diabetes in Pregnancy Study Groups criteria during 24-28 weeks of gestation. Multiple metabolic biomarkers (HOMA-IR (homeostatic model assessment for insulin resistance), HbA1c, c-peptide, high-sensitivity C-reactive protein, adiponectin, leptin, and blood lipids) were additionally measured among 672 non-GDM controls at enrollment.Results: Higher levels of saturated fatty acids (SFAs) 14:0 (pooled odds ratio, 1.41 for each 1-s.d. increase; 95% CI: 1.25, 1.59) and 16:0 (1.19; 1.05, 1.35) were associated with higher odds of GDM. Higher levels of n-6 polyunsaturated fatty acid (PUFA) 18:2n-6 were strongly associated with lower odds of GDM (0.69; 0.60, 0.80). In non-GDM pregnant women, higher SFAs 14:0 and 16:0 but lower n-6 PUFA 18:2n-6 were generally correlated with unfavorable metabolic profiles.Conclusions: We documented adverse associations of 14:0 and 16:0 but a protective association of 18:2n-6 with GDM among Chinese pregnant women. Our findings highlight the distinct roles of specific fatty acids in the onset of GDM.
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20.
  • Petrache, C. M., et al. (author)
  • Highly deformed bands in Nd nuclei : New results and consistent interpretation within the cranked Nilsson-Strutinsky formalism
  • 2019
  • In: Physical Review C. - : American Physical Society (APS). - 2469-9985 .- 2469-9993. ; 100:5
  • Journal article (peer-reviewed)abstract
    • Three new highly-deformed (HD) bands are identified in Nd136 and the highly deformed band of Nd137 is extended at higher spin by four transitions, revealing a band crossing associated with the occupation of the second νi13/2 intruder orbital. Extended cranked Nilsson-Strutinsky calculations are performed for all HD bands observed in Nd134, Nd136, and Nd137, achieving for the first time a consistent interpretation of all HD bands in the Nd nuclei. The new interpretation has significant consequences, like the change of parity of the yrast HD bands of Nd134 and Nd136, and the involvement of two negative-parity neutron intruder orbitals in the configurations of most HD bands. The present experimental results and their theoretical interpretation represent an important step forward in the understanding of the second-minimum excitations in the Nd nuclei.
  •  
21.
  • Petrache, C. M., et al. (author)
  • Signatures of enhanced octupole correlations at high spin in Nd 136
  • 2020
  • In: Physical Review C. - : American Physical Society (APS). - 2469-9985 .- 2469-9993. ; 102:1
  • Journal article (peer-reviewed)abstract
    • Experimental signatures of moderately enhanced octupole correlations at high spin in Nd136 are indicated for the first time. The extracted dipole moments of two negative-parity bands are only two times smaller than those of the lanthanide nuclei with N≈90 which present well-established octupole correlations. Calculations using the cranked quasiparticle random phase approximation and a model of quadrupole-octupole rotations with octupole vibrations reveal the structure of the bands and the enhanced octupole correlations at high spin in Nd136.
  •  
22.
  • Yang, Shangchen, et al. (author)
  • Genomic investigation of the Chinese alligator reveals wild-extinct genetic diversity and genomic consequences of their continuous decline
  • 2023
  • In: Molecular Ecology Resources. - : Wiley. - 1755-098X .- 1755-0998. ; 23:1, s. 294-311
  • Journal article (peer-reviewed)abstract
    • Critically endangered species are usually restricted to small and isolated populations. High inbreeding without gene flow among populations further aggravates their threatened condition and reduces the likelihood of their long-term survival. Chinese alligator (Alligator sinensis) is one of the most endangered crocodiles in the world and has experienced a continuous decline over the past c. 1 million years. In order to identify the genetic status of the remaining populations and aid conservation efforts, we assembled the first high-quality chromosome-level genome of Chinese alligator and explored the genomic characteristics of three extant breeding populations. Our analyses revealed the existence of at least three genetically distinct populations, comprising two breeding populations in China (Changxing and Xuancheng) and one breeding population in an American wildlife refuge. The American population does not belong to the last two populations of its native range (Xuancheng and Changxing), thus representing genetic diversity extinct in the wild and provides future opportunities for genetic rescue. Moreover, the effective population size of these three populations has been continuously declining over the past 20 ka. Consistent with this decline, the species shows extremely low genetic diversity, a large proportion of long runs of homozygous fragments, and mutational load across the genome. Finally, to provide genomic insights for future breeding management and conservation, we assessed the feasibility of mixing extant populations based on the likelihood of introducing new deleterious alleles and signatures of local adaptation. Overall, this study provides a valuable genomic resource and important genomic insights into the ecology, evolution, and conservation of critically endangered alligators. 
  •  
23.
  • Yen, Ying-Tzu, et al. (author)
  • Prominent Enhancement of Cisplatin Efficacy with Optimized Methoxy Poly(ethylene glycol)-Polycaprolactone Block Copolymeric Nanoparticles
  • 2020
  • In: Journal of Biomedical Nanotechnology. - : AMER SCIENTIFIC PUBLISHERS. - 1550-7033 .- 1550-7041. ; 16:3, s. 335-343
  • Journal article (peer-reviewed)abstract
    • Chemotherapy has been one of the major standard treatments for a variety of cancers. cis-Dichlorodiamminoplatiunum(II) (cisplatin, CDDP), as one of the anticancer agents, demonstrated excellent efficacy against tumor and has been an indispensable component in chemotherapy, chemoradiation, chemo-molecular targeted therapy and chemo-immunotherapy. However, its therapeutic concentration was limited since its inevitable toxicity. Previously, we have constructed CDDP-loaded nanoparticles (NPs) with mixture of poly(ethyleneglycol)-polycaprolactone (PEG-PCL) and polycarprolactone (HO-PCL) by a facile method. The most optimal proportion of the two copolymers was selected through a series of physical, chemical, cytological and histological evaluations. In the present study, we explored the mechanisms of NPs and observed the in vivo antitumor effect after administrating CDDP-loaded PEG-PCL NPs. Positron emission tomography as well as computed tomography (PET/CT) were adopted for detecting tumoral metabolic activity. Images from fluorescence microscope revealed superior cellular uptake of CDDP-loaded NPs with rhodamine B aggregated intracellularly in cancer cells. Similar apoptotic rates between free CDDP group and CDDP-loaded NPs group was measured by flow cytometry. Tumor volumes and murine weights confirmed the superiority of CDDP-loaded NPs in therapeutic efficacy as compared with free CDDP. Blood tests showed milder side effects in CDDP-loaded nanoparticle group. PET/CT images illustrated less uptake intensity of FDG in mice received CDDP-loaded NPs than free CDDP. Our results suggest that PEG-PCL/PCL NPs could be a promising antitumor drug carrier for CDDP delivery with solid efficacy and minor side effects.
  •  
24.
  • Yin, Xuan, et al. (author)
  • Achieving ultralow friction under high pressure through operando formation of PbS QDs/graphene heterojunction with 0D/1D nanostructure
  • 2024
  • In: Carbon. - : Elsevier. - 0008-6223 .- 1873-3891. ; 218
  • Journal article (peer-reviewed)abstract
    • In this work, ultralow friction (0.054) of graphene was achieved under high contact pressure (1.03 GPa) and atmosphere environment via the operando formation of PbS quantum dots (QDs)/graphene heterojunction at the frictional interface. It is found that PbS QDs are trapped in graphene nanosheets via shear-induced rearrangement for obtaining the PbS QDs/graphene heterojunctions, which provide an excellent rolling effect to lower friction. It is also found that the heterogeneous PbS QDs/graphene tribofilms have a strong Pb-enriched function and heterojunction nanorod phase. Our objective is to uncover the physical and chemical mechanisms governing the friction of 0D/1D nanostructures within PbS QDs/graphene heterostructures through our studies. This research will enhance our comprehension of nanomaterials' frictional behavior while offering valuable guidance and optimization strategies for their application in mechanical engineering and functional nanomaterials. Consequently, our efforts aim to foster the advancement of nanoscience and technology, leading to additional scientific and technological breakthroughs.
  •  
25.
  • Anderson, Cynthia M., et al. (author)
  • Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2009-31 January 2010
  • 2010
  • In: Molecular Ecology Resources. - : Wiley. - 1755-098X .- 1755-0998. ; 10:3, s. 576-579
  • Journal article (peer-reviewed)abstract
    • This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mulleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus.
  •  
26.
  • Bai, Ru, et al. (author)
  • Clinical characteristics and phylogenetic analysis of human enteric adenovirus type 41 (HAdV-F41) from children with gastroenteritis during SARS-CoV-2 pandemic
  • 2024
  • In: Infection, Genetics and Evolution. - : Elsevier. - 1567-1348 .- 1567-7257. ; 123
  • Journal article (peer-reviewed)abstract
    • Human adenovirus type 41 (HAdV-F41) usually causes pediatrics gastroenteritis. However, it was reported to be associated with the outbreaks of severe acute hepatitis of unknown aetiology (SAHUA) in pediatrics during COVID-19 pandemic. In this study, we investigated the prevalence of enteric HAdV-F41 in 37,920 paediatric gastroenteritis cases from 2017 to 2022 in Guangzhou, China. All children presented were tested negative for SARS-CoV-2 during the “zero-COVID” period. The main clinical symptom of the children was diarrhea (96.5%). No fatalities nor liver abnormal symptoms was found. In 2021, one year since the pandemic of COVID-19, the prevalence of HAdV-F41 abruptly increased from 3.71% to 8.64% (P < 0.001). All of HAdV-F41 circulating worldwide were classified into eight different subtypes (G1-G8) based on the phylogenetic clustering permutation of the four capsid genes of HAdV-F41. G3 was the predominant subtype (56.2%; 77/137). CRV5 isolates from SAHUA cases belong to this subtype, in which N312D and H335D mutations in the short fiber knob were identified in both Guangzhou and CRV5 isolates, presumably changing the virus tropism by directly interacting with the heparin sulfate (HS) receptor. Additionally, a novel recombinant G6 subtype, which is unique and only circulating in China was first identified in this study. This is the first study highlighting the prevalence of HAdV-F41 in paediatric cases of gastroenteritis during COVID-19 pandemic in China. The clinical and viral evolution finding of HAdV-F41 provide insight into the clinical characteristics of children with HAdV-F41 infections as well as the uncertain role of HAdV-F41 in the cause of SAHUA.
  •  
27.
  • Cao, Lina, et al. (author)
  • Atomically dispersed iron hydroxide anchored on Pt for preferential oxidation of CO in H-2
  • 2019
  • In: Nature. - : NATURE PUBLISHING GROUP. - 0028-0836 .- 1476-4687. ; 565:7741, s. 631-635
  • Journal article (peer-reviewed)abstract
    • Proton-exchange-membrane fuel cells (PEMFCs) are attractive next-generation power sources for use in vehicles and other applications(1), with development efforts focusing on improving the catalyst system of the fuel cell. One problem is catalyst poisoning by impurity gases such as carbon monoxide (CO), which typically comprises about one per cent of hydrogen fuel(2-4). A possible solution is on-board hydrogen purification, which involves preferential oxidation of CO in hydrogen (PROX)(3-7). However, this approach is challenging(8-15) because the catalyst needs to be active and selective towards CO oxidation over a broad range of low temperatures so that CO is efficiently removed (to below 50 parts per million) during continuous PEMFC operation (at about 353 kelvin) and, in the case of automotive fuel cells, during frequent cold-start periods. Here we show that atomically dispersed iron hydroxide, selectively deposited on silica-supported platinum (Pt) nanoparticles, enables complete and 100 per cent selective CO removal through the PROX reaction over the broad temperature range of 198 to 380 kelvin. We find that the mass-specific activity of this system is about 30 times higher than that of more conventional catalysts consisting of Pt on iron oxide supports. In situ X-ray absorption fine-structure measurements reveal that most of the iron hydroxide exists as Fe-1(OH)(x) clusters anchored on the Pt nanoparticles, with density functional theory calculations indicating that Fe-1(OH)(x)-Pt single interfacial sites can readily react with CO and facilitate oxygen activation. These findings suggest that in addition to strategies that target oxide-supported precious-metal nanoparticles or isolated metal atoms, the deposition of isolated transition-metal complexes offers new ways of designing highly active metal catalysts.
  •  
28.
  • Chen, Song, et al. (author)
  • Early osteoimmunomodulation by mucin hydrogels augments the healing and revascularization of rat critical-size calvarial bone defects
  • 2023
  • In: BIOACTIVE MATERIALS. - : Elsevier BV. - 2452-199X. ; 25, s. 176-188
  • Journal article (peer-reviewed)abstract
    • The design principle of osteogenic bone grafts has shifted from immunological inertness to limiting foreign body response to combined osteoimmunomodulatory activity to promote high-quality endogenous bone regeneration. Recently developed immunomodulatory mucin hydrogels have been shown to elicit very low complement activation and suppress macrophage release and activation after implantation in vivo. However, their immunoregulatory activity has not yet been studied in the context of tissue repair. Herein, we synthesized mucinmonetite composite materials and investigated their early osteoimmunomodulation using a critical-size rat bone defect model. We demonstrated that the composites can polarize macrophages towards the M2 phenotype at weeks 1 and 2. The early osteoimmunomodulation enhanced early osteogenesis and angiogenesis and ultimately promoted fracture healing and engraftment (revascularization of the host vasculature) at weeks 6 and 12. Overall, we demonstrated the applicability of mucin-based immunomodulatory biomaterials to enhance tissue repair in tissue engineering and regenerative medicine.
  •  
29.
  • Chen, W., et al. (author)
  • Enhanced efficiency of polymer solar cells by improving molecular aggregation and broadening the absorption spectra
  • 2019
  • In: Dyes and Pigments. - : Elsevier BV. - 0143-7208 .- 1873-3743. ; 166, s. 42-48
  • Journal article (peer-reviewed)abstract
    • Alkylthio-substituted thiophene-based benzo[1,2-b:4,5-b’]dithiophene (BDT) was used to construct PBDTS-DTBT, a medium band gap donor-acceptor (D-A) polymer with 5,6-difluoro-4,7-bis[4-(2-octyldodecyl)thiophene-2-yl]benzo[c] [1,2,5]thiadiazole (DTBT). The incorporation of sulfur atoms into the side chains not only lowered the highest occupied molecular orbital (HOMO) energy level but also improved molecular aggregation and thus afforded lower band gap (1.68 eV) with full width at half maximum (FWHM) of 170 nm in comparison to that of the analogous polymer (PBDT-DTBT) without sulfur atoms in side chains. Therefore, the bulk heterojunction polymer solar cells based on PBDTS-DTBT with 2% diiodooctane (DIO) as processing additive showed a high power conversion efficiency (PCE) of 9.73% with high open-circuit voltage (V OC , 0.93 V), large short-circuit current density (J SC , 14.23 mA/cm 2 ) and high fill factor (FF, 0.735).
  •  
30.
  • Chen, Weiwei, et al. (author)
  • Lane departure warning systems and lane line detection methods based on image processing and semantic segmentation : A review
  • 2020
  • In: Journal of Traffic and Transportation Engineering (English Edition). - : Elsevier BV. - 2095-7564. ; 7:6, s. 748-774
  • Research review (peer-reviewed)abstract
    • Recently, the development and application of lane line departure warning systems have been in the market. For any of the systems, the key part of lane line tracking, lane line identification, or lane line departure warning is whether it can accurately and quickly detect lane lines. Since 1990s, they have been studied and implemented for the situations defined by the good viewing conditions and the clear lane markings on road. After then, the accuracy for particular situations, the robustness for a wide range of scenarios, time efficiency and integration into higher-order tasks define visual lane line detection and tracking as a continuing research subject. At present, these kinds of lane marking line detection methods based on machine vision and image processing can be divided into two categories: the traditional image processing and semantic segmentation (includes deep learning) methods. The former mainly involves feature-based and model-based steps, and which can be classified into similarity- and discontinuity-based ones; and the model-based step includes different parametric straight line, curve or pattern models. The semantic segmentation includes different machine learning, neural network and deep learning methods, which is the new trend for the research and application of lane line departure warning systems. This paper describes and analyzes the lane line departure warning systems, image processing algorithms and semantic segmentation methods for lane line detection.
  •  
31.
  • Costa, Diogo Ribeiro, et al. (author)
  • Coated UN microspheres embedded in UO2 matrix as an innovative advanced technology fuel: Early progress
  • 2021
  • In: TopFuel 2021 Light Water Reactor Fuel Performance Conference, Santander, Spain, October 24-28, 2021..
  • Conference paper (peer-reviewed)abstract
    • Uranium nitride (UN)-uranium dioxide (UO2) composites have been proposed as an innovative advanced technology fuel (ATF) option for light water reactors (LWRs). However, the interdiffusion of oxygen and nitrogen during fabrication result in the formation of α-U2N3. A way to avoid this interaction is to coat the UN with a material that is impermeable to oxygen and nitrogen, has a high melting point, high thermal conductivity, and reasonable low neutron cross-section. Among many candidates,refractory metals may be the first option. In this study, we present an early progressresult of fabricating an innovative ATF concept: coated UN microspheres embedded in UO2 matrix. To do so, the following steps are performed: 1) diffusion couple experiments of UN-X-UO2 (X=W, Mo, Ta, Nb, V) to evaluate the interactions between the coating candidates (X) and the fuels; 2) selection of the most promising candidates; 3) use a surrogate material (ZrN microspheres) to develop processes to coat the microspheres with nanopowders: dry and wet methods; 4) coating the UN microspheres with a selected method; 5) finally, sinter a coated UN-UO2 composite using spark plasma sintering (SPS), and compare the results with an uncoated UNUO2 composite sintered at the same SPS conditions (1500 °C, 80 MPa, 3 min,vacuum). The diffusion couple results indicate W and Mo as the most promising candidates, with the wet method showing the smoothest surface. So, dense (~95 %TD) W/UN-UO2 and Mo/UN-UO2 were sintered and the preliminary results show that the tungsten coating was not efficient due to poor adhesion. Conversely, the Mo coating (~15 µm) was efficient against the α-U2N3 formation. Therefore, this early progress indicates the possibility of fabricating an innovative ATF concept using a low cost and potentially applicable coating method.
  •  
32.
  •  
33.
  • DeFilipp, Zachariah, et al. (author)
  • Maintenance Tyrosine Kinase Inhibitors Following Allogeneic Hematopoietic Stem Cell Transplantation for Chronic Myelogenous Leukemia : A Center for International Blood and Marrow Transplant Research Study
  • 2020
  • In: Biology of blood and marrow transplantation. - : Elsevier. - 1083-8791 .- 1523-6536. ; 26:3, s. 472-479
  • Journal article (peer-reviewed)abstract
    • It remains unknown whether the administration of tyrosine kinase inhibitors (TKIs) targeting BCR-ABL1 after allogeneic hematopoietic cell transplantation (HCT) is associated with improved outcomes for patients with chronic myelogenous leukemia (CML). In this registry study, we analyzed clinical outcomes of 390 adult patients with CML who underwent transplantation between 2007 and 2014 and received maintenance TKI following HCT (n = 89) compared with no TKI maintenance (n = 301), as reported to the Center for International Blood and Marrow Transplant Research. All patients received TKI therapy before HCT. The majority of patients had a disease status of first chronic phase at HCT (n = 240; 62%). The study was conducted as a landmark analysis, excluding patients who died, relapsed, had chronic graft-versus-host disease, or were censored before day +100 following HCT. Of the 89 patients who received TKI maintenance, 77 (87%) received a single TKI and the other 12 (13%) received multiple sequential TKIs. The most common TKIs used for maintenance were dasatinib (n = 50), imatinib (n = 27), and nilotinib (n = 27). As measured from day +100, the adjusted estimates for 5-year relapse (maintenance, 35% versus no maintenance, 26%; P = .11), leukemia-free survival (maintenance, 42% versus no maintenance, 44%; P = .65), or overall survival (maintenance, 61% versus no maintenance, 57%; P = .61) did not differ significantly between patients receiving TKI maintenance or no maintenance. These results remained unchanged in multivariate analysis and were not modified by disease status before transplantation. In conclusion, our data from this day +100 landmark analysis do not demonstrate a significant impact of maintenance TKI therapy on clinical outcomes. The optimal approach to TKI administration in the post-transplantation setting in patients with CML remains undetermined.
  •  
34.
  • Deng, Huan, et al. (author)
  • Altered Expression of the Hedgehog Pathway Proteins BMP2, BMP4, SHH, and IHH Involved in Knee Cartilage Damage of Patients With Osteoarthritis and Kashin-Beck Disease
  • 2022
  • In: Cartilage. - : Sage Publications. - 1947-6035 .- 1947-6043. ; 13:1
  • Journal article (peer-reviewed)abstract
    • OBJECTIVE: To investigate the expression of Hedgehog (HH) signaling pathway proteins in knee articular cartilage from Kashin-Beck disease (KBD) and osteoarthritis (OA) patients.METHODS: Knee articular cartilage samples were collected from normal (N), OA, and KBD adults (aged 38-60 years) and divided into 3 groups with 6 subjects in each group. The localization of the HH pathway proteins bone morphogenetic protein 2 (BMP2), bone morphogenetic protein 4 (BMP4), Sonic hedgehog (SHH), and Indian hedgehog (IHH) was observed with the microscope after immunohistochemical (IHC) staining. Positive staining cell rates of each proteins were compared.RESULTS: The strongest stainings of all proteins were observed in the middle zones of all 3 groups. The positive staining rates of BMP4 and IHH were significantly lower in the OA and KBD groups than those in the N group in all 3 zones. The positive staining rates of BMP2 and SHH tend to be lower in the OA and KBD groups than those in the N group in the deep zone, while higher in the OA and KBD groups than those in the N group in superficial and middle zones.CONCLUSIONS: Altered expression of the HH pathway proteins BMP2, BMP4, SHH, and IHH was found in OA and KBD articular cartilage. There seemed to be a compensatory effect between SHH and IHH in cartilage damage. Further studies on the pathogenesis of OA and KBD may be carried out from these aspects in the future.
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35.
  • Deng, Huan, et al. (author)
  • Progress of selenium deficiency in the pathogenesis of arthropathies and selenium supplement for their treatment
  • 2022
  • In: Biological Trace Element Research. - : Springer Nature. - 0163-4984 .- 1559-0720. ; 200, s. 4238-4249
  • Research review (peer-reviewed)abstract
    • Selenium, an essential trace element for human health, exerts an indispensable effect in maintaining physiological homeostasis and functions in the body. Selenium deficiency is associated with arthropathies, such as Kashin-Beck disease, rheumatoid arthritis, osteoarthritis, and osteoporosis. Selenium deficiency mainly affects the normal physiological state of bone and cartilage through oxidative stress reaction and immune reaction. This review aims to explore the role of selenium deficiency and its mechanisms existed in the pathogenesis of arthropathies. Meanwhile, this review also summarized various experiments to highlight the crucial functions of selenium in maintaining the homeostasis of bone and cartilage.
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36.
  • Fang, Shifeng, et al. (author)
  • Water Resources Assessment and Regional Virtual Water Potential in the Turpan Basin, China
  • 2010
  • In: Water resources management. - : Springer Science and Business Media LLC. - 0920-4741 .- 1573-1650. ; 24:13, s. 3321-3332
  • Journal article (peer-reviewed)abstract
    • Located in the centre of the Eurasian Continent, the Turpan Basin, as the second deepest lowland in the world, is extremely short of water resources. Aimed at this key scientific issue, this paper based on years of meteorological and hydrological observation data, carried out a scientific calculation and evaluation of surface and groundwater resources in the Turpan Basin, and then, with the help of modified Penman formula, calculated the virtual water potential in the basin in 2004. The results show that the average total usable surface water resources per year in the last decade were about 6.673x10(8) m(3), while adduction volume of surface water in 2003 was about 4.94x10(8) m(3), which means that most of the region has reached or approached the limit of water resources and, as a result serious crises and constraints on the development of the basin were thus caused. The exploitation content of groundwater in the Turpan Basin in 2003 was about 6.12x10(8) m(3), which has basically reached its upper limit, and the ground water level has fallen about 10 similar to 40 m in the Turpan Basin in recent years. The daily reference crop water requirement in the Turpan Basin in 2004 was about 1,053.39 mm, and the total virtual water potential contained in six main crops was about 5.25 x10(8) m(3) in 2004. All these showed that research works on scientific assessment of water resources and regional virtual water strategy have great significance for the best social, ecological, economic benefits and regional sustainable development of the Turpan Basin.
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37.
  • Gonzalez Fonseca, Luis Guillermo, 1993, et al. (author)
  • Application of SPS in the fabrication of UN and (U,Th)N pellets from microspheres
  • 2020
  • In: Journal of Nuclear Materials. - : Elsevier BV. - 0022-3115 .- 1873-4820. ; 536
  • Journal article (peer-reviewed)abstract
    • In this study, the process involved in the fabrication of a potential accident tolerant fuel is described. Homogeneous uranium nitride microspheres doped with different thorium content were successfully manufactured using an internal gelation process followed by carbothermic reduction, and nitridation. Elemental analysis of the materials showed low carbon and oxygen content, the two major impurities found in the products of carbothermic reduction. Uranium nitride microspheres were pressed and sintered using spark plasma sintering (SPS) to produce pellets with variable density. Final density can be tailored by choosing the sintering temperature, pressure and time. Density values of 77–98% of theoretical density (%TD) were found. As expected, higher temperatures and pressures resulted in a denser material. Furthermore, a direct correlation between the onset sintering temperature and thorium content in the materials was observed. The change of onset temperature has been related to an increment in the activation energy for self-diffusion due to the substitution of uranium atoms by thorium in the crystal structure.
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38.
  • Guo, Feilong, et al. (author)
  • How Early-Life Gut Microbiota Alteration Sets Trajectories for Health and Inflammatory Bowel Disease?
  • 2021
  • In: Frontiers in Nutrition. - : Frontiers Media S.A.. - 2296-861X. ; 8
  • Research review (peer-reviewed)abstract
    • Inflammatory bowel disease (IBD) is a recurrent chronic inflammatory condition of the intestine without any efficient therapeutic regimens. Gut microbiota, which plays an instrumental role in the development and maturation of the immune system, has been implicated in the pathogenesis of IBD. Emerging evidence has established that early-life events particularly maternal influences and antibiotic treatment are strongly correlated with the health or susceptibility to disease of an individual in later life. Thus, it is proposed that there is a critical period in infancy, during which the environmental exposures bestow a long-term pathophysiological imprint. This notion sheds new light on the development of novel approaches for the treatment, i.e., early interventions, more precisely, the prevention of many uncurable chronic inflammatory diseases like IBD. In this review, we have integrated current evidence to describe the feasibility of the "able-to-be-regulated microbiota," summarized the underlying mechanisms of the "microbiota-driven immune system education," explored the optimal intervention time window, and discussed the potential of designing early-probiotic treatment as a new prevention strategy for IBD.
  •  
39.
  • Gupta, Vikas, et al. (author)
  • Comparison of outcomes of HCT in blast phase of BCR-ABL1- MPN with de novo AML and with AML following MDS
  • 2020
  • In: Blood Advances. - : American Society of Hematology. - 2473-9529 .- 2473-9537. ; 4:19, s. 4748-4757
  • Journal article (peer-reviewed)abstract
    • Comparative outcomes of allogeneic hematopoietic cell transplantation (HCT) for BCR-ABL12 myeloproliferative neoplasms (MPNs) in blast phase (MPN-BP) vs de novo acute myeloid leukemia (AML), and AML with prior myelodysplastic syndromes (MDSs; post-MDS AML), are unknown. Using the Center for International Blood and Marrow Transplant Research (CIBMTR) database, we compared HCT outcomes in 177 MPN-BP patients with 4749 patients with de novo AML, and 1104 patients with post-MDS AML, using multivariate regression analysis in 2 separate comparisons. In a multivariate Cox model, no difference in overall survival (OS) or relapse was observed in patients with MPN-BP vs de novo AML with active leukemia at HCT. Patients with MPN-BP in remission had inferior OS in comparison with de novo AML in remission (hazard ratio [HR], 1.40 [95% confidence interval [ CI], 1.12-1.76]) due to higher relapse rate (HR, 2.18 [95% CI, 1.69-2.80]). MPN-BP patients had inferior OS (HR, 1.19 [95% CI, 1.00-1.43]) and increased relapse (HR, 1.60 [95% CI, 1.31-1.96]) compared with post-MDS AML. Poor-risk cytogenetics were associated with increased relapse in both comparisons. Peripheral blood grafts were associated with decreased relapse in MPN-BP and post-MDS AML (HR, 0.70 [95% CI, 0.57-0.86]). Nonrelapse mortality (NRM) was similar between MPN-BP vs de novo AML, and MPN-BP vs post-MDS AML. Total-body irradiation-based myeloablative conditioning was associated with higher NRM in both comparisons. Survival of MPN-BP after HCT is inferior to de novo AML in remission and post-MDS AML due to increased relapse. Relapse-prevention strategies are required to optimize HCT outcomes in MPN-BP.
  •  
40.
  • He, Yafang, et al. (author)
  • Metabolomic Changes Upon Conjugated Linoleic Acid Supplementation and Predictions of Body Composition Responsiveness
  • 2022
  • In: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 1945-7197 .- 0021-972X. ; 107:9, s. 2606-2615
  • Journal article (peer-reviewed)abstract
    • Context Conjugated linoleic acid (CLA) may optimize body composition, yet mechanisms underlining its benefits are not clear in humans. Objective We aimed to reveal the CLA-induced changes in the plasma metabolome associated with body composition improvement and the predictive performance of baseline metabolome on intervention responsiveness. Methods Plasma metabolome from overnight fasted samples at pre- and post-intervention of 65 participants in a 12-week randomized, placebo-controlled trial (3.2 g/day CLA vs 3.2 g/day sunflower oil) were analyzed using untargeted LC-MS metabolomics. Mixed linear model and machine learning were applied to assess differential metabolites between treatments, and to identify optimal panel (based on baseline conventional variables vs metabolites) predicting responders of CLA-derived body composition improvement (increased muscle variables or decreased adiposity variables) based on dual-energy x-ray absorptiometry. Results Compared with placebo, CLA altered 57 metabolites (P
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41.
  • Hedman, Åsa K., et al. (author)
  • Epigenetic Patterns in Blood Associated With Lipid Traits Predict Incident Coronary Heart Disease Events and Are Enriched for Results From Genome-Wide Association Studies
  • 2017
  • In: Circulation. - : LIPPINCOTT WILLIAMS & WILKINS. - 1942-325X .- 1942-3268. ; 10:1
  • Journal article (peer-reviewed)abstract
    • Background- Genome-wide association studies have identified loci influencing circulating lipid concentrations in humans; further information on novel contributing genes, pathways, and biology may be gained through studies of epigenetic modifications. Methods and Results- To identify epigenetic changes associated with lipid concentrations, we assayed genome-wide DNA methylation at cytosine-guanine dinucleotides (CpGs) in whole blood from 2306 individuals from 2 population-based cohorts, with replication of findings in 2025 additional individuals. We identified 193 CpGs associated with lipid levels in the discovery stage (P < 1.08E-07) and replicated 33 (at Bonferroni-corrected P < 0.05), including 25 novel CpGs not previously associated with lipids. Genes at lipid-associated CpGs were enriched in lipid and amino acid metabolism processes. A differentially methylated locus associated with triglyceridesand high-density lipoprotein cholesterol (HDL- C; cg27243685; P= 8.1E-26 and 9.3E-19) was associated with cis-expression of a reverse cholesterol transporter (ABCG1; P= 7.2E-28) and incident cardiovascular disease events (hazard ratio per SD increment, 1.38; 95% confidence interval, 1.15-1.66; P= 0.0007). We found significant cis-methylation quantitative trait loci at 64% of the 193 CpGs with an enrichment of signals from genome-wide association studies of lipid levels (P-TC = 0.004, PHDL-C = 0.008 and P-triglycerides = 0.00003) and coronary heart disease ( P= 0.0007). For example, genome-wide significant variants associated with low-density lipoprotein cholesterol and coronary heart disease at APOB were cis-methylation quantitative trait loci for a low-density lipoprotein cholesterol-related differentially methylated locus. Conclusions-We report novel associations of DNA methylation with lipid levels, describe epigenetic mechanisms related to previous genome-wide association studies discoveries, and provide evidence implicating epigenetic regulation of reverse cholesterol transport in blood in relation to occurrence of cardiovascular disease events.
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42.
  • Huan, Tianxiao, et al. (author)
  • Genome-wide identification of DNA methylation QTLs in whole blood highlights pathways for cardiovascular disease
  • 2019
  • In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10
  • Journal article (peer-reviewed)abstract
    • Identifying methylation quantitative trait loci (meQTLs) and integrating them with disease-associated variants from genome-wide association studies (GWAS) may illuminate functional mechanisms underlying genetic variant-disease associations. Here, we perform GWAS of >415 thousand CpG methylation sites in whole blood from 4170 individuals and map 4.7 million cis- and 630 thousand trans-meQTL variants targeting >120 thousand CpGs. Independent replication is performed in 1347 participants from two studies. By linking cis-meQTL variants with GWAS results for cardiovascular disease (CVD) traits, we identify 92 putatively causal CpGs for CVD traits by Mendelian randomization analysis. Further integrating gene expression data reveals evidence of cis CpG-transcript pairs causally linked to CVD. In addition, we identify 22 trans-meQTL hotspots each targeting more than 30 CpGs and find that trans-meQTL hotspots appear to act in cis on expression of nearby transcriptional regulatory genes. Our findings provide a powerful meQTL resource and shed light on DNA methylation involvement in human diseases.
  •  
43.
  • Huang, Boming, et al. (author)
  • IECA : An In-Execution Configuration CNN Accelerator With 30.55 GOPS/mm(2) Area Efficiency
  • 2021
  • In: IEEE Transactions on Circuits and Systems Part 1. - : Institute of Electrical and Electronics Engineers (IEEE). - 1549-8328 .- 1558-0806. ; 68:11, s. 4672-4685
  • Journal article (peer-reviewed)abstract
    • It remains challenging for a Convolutional Neural Network (CNN) accelerator to maintain high hardware utilization and low processing latency with restricted on-chip memory. This paper presents an In-Execution Configuration Accelerator (IECA) that realizes an efficient control scheme, exploring architectural data reuse, unified in-execution controlling, and pipelined latency hiding to minimize configuration overhead out of the computation scope. The proposed IECA achieves row-wise convolution with tiny distributed buffers and reduces the size of total on-chip memory by removing 40% of redundant memory storage with shared delay chains. By exploiting a reconfigurable Sequence Mapping Table (SMT) and Finite State Machine (FSM) control, the chip realizes cycle-accurate Processing Element (PE) control, automatic loop tiling and latency hiding without extra time slots for pre-configuration. Evaluated on AlexNet and VGG-16, the IECA retains over 97.3% PE utilization and over 95.6% memory access time hiding on average. The chip is designed and fabricated in a UMC 55-nm process running at a frequency of 250 MHz and achieves an area efficiency of 30.55 GOPS/mm(2) and 0.244 GOPS/KGE (kilo-gate-equivalent), which makes an over 2.0x and 2.1x improvement, respectively, compared with that of previous related works. Implementation of the IEC control scheme uses only a 0.55% area of the 2.75 mm(2) core.
  •  
44.
  • Huo, Huan, et al. (author)
  • Anomalous Region Detection on the Mobility Data
  • 2015
  • In: CIT/IUCC/DASC/PICOM 2015 IEEE INTERNATIONAL CONFERENCE ON COMPUTER AND INFORMATION TECHNOLOGY - UBIQUITOUS COMPUTING AND COMMUNICATIONS - DEPENDABLE, AUTONOMIC AND SECURE COMPUTING - PERVASIVE INTELLIGENCE AND COMPUTING. - : Institute of Electrical and Electronics Engineers (IEEE). - 9781509001545 ; , s. 1669-1674
  • Conference paper (peer-reviewed)abstract
    • Mobility data records the change of location and time about the crowd activities, reflecting a large amount of semantic knowledge about human mobility and hot regions. From the perspective of regional semantic knowledge, mining anomalous regions of overcrowded area is essential for disaster-aware resilience system scheme. This paper studies how to discover anomalous regions of moving crowds over the mobility data. From the perspective of spatial information analysis about the location sequence of moving crowds, the paper introduces grid structure to index activity space and proposes a density calculation method of grid cells based on kernel function. By adopting Top-k sorting method, the algorithm determines the density thresholds to detect the anomalous regions. Finally, experimental results validate the feasibility and effectiveness of the above method on practical data sets.
  •  
45.
  • Jing, Chun-lei, et al. (author)
  • Avian malaria parasite infections do not affect personality in the chestnut thrush (Turdus rubrocanus) on the Qinghai-Tibet Plateau
  • 2023
  • In: Heliyon. - : Cell Press. - 2405-8440. ; 9:9
  • Journal article (peer-reviewed)abstract
    • Personality traits, the consistent individual behavioral differences, are currently gaining much attention in studies of natural bird populations. However, associations between personality traits and parasite infections are not often investigated. Even less attention has been given to studies of birds in the high-elevation region such as the Tibetan plateau. This research aims to examine the relationship between avian malaria parasites and two personality traits in a population of the Chestnut Thrush (Turdus rubrocanus) breed in the Tibetan plateau. Our results revealed no evidence of sex bias in malaria parasite prevalence. Furthermore, we found no effect of infection status on two personality scores: activity and boldness. Additionally, no effects on the activity level or boldness were observed for different parasite lineages of Haemoproteus, Leucocytozoon, the sex of the birds, or their interactions. Similarly, we did not find any relationship between activity level and boldness with nestling numbers, sex, or their interactions. Notably, individuals with a larger number of offspring tended to display greater boldness. Our findings indicate that blood parasite infections are common in this population but do not significantly impact the personality of the birds.
  •  
46.
  • Klionsky, Daniel J., et al. (author)
  • Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
  • 2008
  • In: Autophagy. - : Landes Bioscience. - 1554-8627 .- 1554-8635. ; 4:2, s. 151-175
  • Research review (peer-reviewed)abstract
    • Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
  •  
47.
  • Li, Wenting, et al. (author)
  • Recent progress in silver nanowire networks for flexible organic electronics
  • 2020
  • In: Journal of Materials Chemistry C. - : ROYAL SOC CHEMISTRY. - 2050-7526 .- 2050-7534. ; 8:14, s. 4636-4674
  • Research review (peer-reviewed)abstract
    • Recently, flexible transparent electrodes (FTEs) have attracted extensive attention as an essential element for future organic electronics (OEs), i.e. solution-processable, scalable and flexible organic electronics (FOEs). Although the traditional transparent electrode indium tin oxide (ITO) has been widely used in OEs, its brittleness and high cost significantly limit its application in the next generation of devices, typically flexible electronics. Thus, many alternatives, such as graphene, carbon nanotubes, conductive polymers, metal nanowires, metal grids and electrospun metallic nanofibers, have arisen at the forefront of FTEs. Among them, silver nanowire (AgNW) networks have attracted particular attention due to their excellent electrical conductivity and high transmittance, as well as facile availability and low cost. Since many studies on AgNWs have been published, a comprehensive review highlighting the advantages of AgNWs in FOEs is highly required. In this review, the synthesis and film fabrication of AgNWs have been firstly summarized, focusing especially on the properties of conductivity and light transmittance. Next, post treatments with different approaches to improve the conductivity of AgNWs have been included. And then, characterization of FTEs has been introduced with details on key parameters for FOEs. Furthermore, AgNW-based FOEs have been summarized to demonstrate the recent progress, such as organic light-emitting diodes (OLEDs), organic solar cells (OSCs), light-emitting electrochemical cells (LECs), organic field effect transistors (OFETs), organic memory devices (OMDs), etc. Finally, perspectives for AgNWs in FOEs have been discussed and concluded as well. It is expected that AgNWs could be the focus of future FOEs compared with other alternatives in terms of their advantages of optoelectronic properties, film-formation, solution-processability and flexibility.
  •  
48.
  • Li, Yuchen, et al. (author)
  • Public awareness, emotional reactions and human mobility in response to the COVID-19 outbreak in China : a population-based ecological study
  • 2022
  • In: Psychological Medicine. - : Cambridge University Press. - 0033-2917 .- 1469-8978. ; 52:9, s. 1793-1800
  • Journal article (peer-reviewed)abstract
    • Background: The outbreak of COVID-19 generated severe emotional reactions, and restricted mobility was a crucial measure to reduce the spread of the virus. This study describes the changes in public emotional reactions and mobility patterns in the Chinese population during the COVID-19 outbreak.Methods: We collected data on public emotional reactions in response to the outbreak through Weibo, the Chinese Twitter, between January 1st and March 31st, 2020. Using anonymized location-tracking information, we analyzed the daily mobility patterns of approximately 90% of Sichuan residents.Results: There were three distinct phases of the emotional and behavioral reactions to the COVID-19 outbreak. The alarm phase (January 19th –26th) was a restriction-free period, characterized by few new daily cases, but enormous public negative emotions (the number of negative comments per Weibo post increased by 246.9 per day, 95%CI: 122.5–371.3), and a substantial increase in self-limiting mobility (from 45.6% to 54.5%, changing by 1.5% per day, 95%CI: 0.7%–2.3%). The epidemic phase (January 27th –February 15th) exhibited rapidly increasing numbers of new daily cases, decreasing expression of negative emotions (a decrease of 27.3 negative comments per post per day, 95%CI: −40.4–−14.2), and a stabilized level of self-limiting mobility. The relief phase (February 16th –March 31st) had a steady decline in new daily cases and decreasing levels of negative emotion and self-limiting mobility.Conclusions: During the COVID-19 outbreak in China, the public’s emotional reaction was strongest before the actual peak of the outbreak and declined thereafter. The change in human mobility patterns occurred before the implementation of restriction orders, suggesting a possible link between emotion and behavior.
  •  
49.
  • Liang, Yusen, et al. (author)
  • Migrating photon avalanche in different emitters at the nanoscale enables 46th-order optical nonlinearity
  • 2022
  • In: Nature Nanotechnology. - : Springer Nature. - 1748-3387 .- 1748-3395. ; 17:5, s. 524-530
  • Journal article (peer-reviewed)abstract
    • A photon avalanche (PA) effect that occurs in lanthanide-doped solids gives rise to a giant nonlinear response in the luminescence intensity to the excitation light intensity. As a result, much weaker lasers are needed to evoke such PAs than for other nonlinear optical processes. Photon avalanches are mostly restricted to bulk materials and conventionally rely on sophisticated excitation schemes, specific for each individual system. Here we show a universal strategy, based on a migrating photon avalanche (MPA) mechanism, to generate huge optical nonlinearities from various lanthanide emitters located in multilayer core/shell nanostructrues. The core of the MPA nanoparticle, composed of Yb3+ and Pr3+ ions, activates avalanche looping cycles, where PAs are synchronously achieved for both Yb3+ and Pr3+ ions under 852 nm laser excitation. These nanocrystals exhibit a 26th-order nonlinearity and a clear pumping threshold of 60 kW cm−2. In addition, we demonstrate that the avalanching Yb3+ ions can migrate their optical nonlinear response to other emitters (for example, Ho3+ and Tm3+) located in the outer shell layer, resulting in an even higher-order nonlinearity (up to the 46th for Tm3+) due to further cascading multiplicative effects. Our strategy therefore provides a facile route to achieve giant optical nonlinearity in different emitters. Finally, we also demonstrate applicability of MPA emitters to bioimaging, achieving a lateral resolution of ~62 nm using one low-power 852 nm continuous-wave laser beam.
  •  
50.
  • Lin, Xialu, et al. (author)
  • Individual and combined toxicity of T-2 toxin and deoxynivalenol on human C-28/I2 and rat primary chondrocytes
  • 2019
  • In: Journal of Applied Toxicology. - : John Wiley & Sons. - 0260-437X .- 1099-1263. ; 39:2, s. 343-353
  • Journal article (peer-reviewed)abstract
    • Deoxynivalenol (DON) and T-2 toxin are prevalent mycotoxin contaminants in the food and feed stuffs worldwide, with non-negligible co-contamination and co-exposure conditions. Meanwhile, they are considerable risk factors for Kashin-Beck disease, a chronic endemic osteochondropathy. The aim of this study was to investigate the individual and combined cytotoxicity of DON and T-2 toxin on proliferating human C-28/I2 and newborn rat primary costal chondrocytes by MTT assay. Four molar concentration combination ratios of DON and T-2 toxin were used, 1:1 for R1 mixture, 10:1 for R10, 100:1 for R100 and 1000:1 for R1000. The toxicological interactions were quantified by the MixLow method. DON, T-2 toxin, and their mixtures all showed a clear dose-dependent toxicity for chondrocytes. The cytotoxicity of T-2 toxin was 285-fold higher than DON was in human chondrocytes, and 22-fold higher in the rat chondrocytes. The combination of DON and T-2 toxin was significantly synergistic at middle and high level concentrations of R10 mixtures in rat chondrocytes, but significantly antagonistic at the low concentrations of R100 mixtures in both cells and at the middle concentrations of R1000 mixtures in rat chondrocytes. These results indicated that the combined toxicity was influenced by the cell sensitivity for toxins, the difference between the combination ratio and equitoxic ratio, the concentrations and other factors.
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