| 1. |
- Desale, Prithviraj, et al.
(author)
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Biosorption of Heavy Metals by Actinomycetes for Treatment of Industrial Effluents
- 2012
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Conference paper (peer-reviewed)abstract
- Rapid industrialization usually is the cause of increase in pollutants in the natural river bodies. This pollution is attributed to lack of improper treatment and inadequate disposal mechanisms of the waste generated thereof. The aim of present investigation was to screen actinomycetes for their ability to adsorb heavy metals. Actinomycetes belong to most diverse group of filamentous prokaryotes with ubiquitous presence in different ecological niches. This ubiquity is due to their ability to degrade almost any type of polymer and to use several compounds as carbon sources. Nearly 200 actinomycetes from different niches like fresh water, soda lake, geothermal springs, acid soils, etc from different locations in India were screened for ability to grow in presence of 1 to 5 mM of heavy metals like Cd, Hg, Pb, Ni, Sr, Fe, Mo, Zn and Mn. Most of the strains tolerant to heavy metals belonged to the genus Streptomyces spp. Five strains could grow in presence of 5 mM Cd2+, 4 strains in presence of 0.1 mM Hg2+, 18 strains in presence of 5 mM Pb2+, 21 strains in presence of 5 mM Ni2+, whereas 34 strains grew in presence of 5 mM Fe2+. One of the most promising strain was studied for its ability to absorb cadmium where 2x1011 cells or spores could adsorb 0.32 mM to 0.33 mM of cadmium. Spores and vegetative cells showed nearly similar level of adsorbtion ability. This strain can be very useful in developing a bioreactor for the adsorption of heavy metals from industrial effluents containing cadmium.
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| 2. |
- Desale, Prithviraj, et al.
(author)
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Biosorption of nickel by Lysinibacillus sp. BA2 native to bauxite mine
- 2014
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In: Ecotoxicology and Environmental Safety. - : Elsevier. - 0147-6513 .- 1090-2414. ; 107, s. 260-268
-
Journal article (peer-reviewed)abstract
- The current scenario of environmental pollution urges the need for an effective solution for toxic heavy metal removal from industrial wastewater. Bioremediation is the most cost effective process employed by the use of microbes especially bacteria resistant to toxic metals. In this study, Lysinibacillus sp. BA2, a nickel tolerant strain isolated from bauxite mine was used for the biosorption of Ni(II). Lysinibacillus sp. BA2 biomass had isoelectric point (pI) of 3.3. The maximum negative zeta potential value (−39.45) was obtained at pH 6.0 which was highly favourable for Ni(II) biosorption. 238.04 mg of Ni(II) adsorbed on one gram of dead biomass and 196.32 mg adsorbed on one gram of live biomass. The adsorption of Ni(II) on biomass increased with time and attained saturation after 180 min with rapid biosorption in initial 30 min. The Langmuir and Freundlich isotherms could fit well for biosorption of Ni(II) by dead biomass while Langmuir isotherm provided a better fit for live biomass based on correlation coefficient values. The kinetic studies of Ni(II) removal, using dead and live biomass was well explained by second-order kinetic model. Ni(II) adsorption on live biomass was confirmed by SEM-EDX where cell aggregation and increasing irregularity of cell morphology was observed even though cells were in non-growing state. The FTIR analysis of biomass revealed the presence of carboxyl, hydroxyl and amino groups, which seem responsible for biosorption of Ni(II). The beads made using dead biomass of Lysinibacillus sp. BA2 could efficiently remove Ni(II) from effluent solutions. These microbial cells can substitute expensive methods for treating nickel contaminated industrial wastewaters.
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| 3. |
- Junnarkar, Manisha, et al.
(author)
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Probiotic potential of lactic acid bacteria from fresh vegetables : Application in food preservation
- 2019
-
In: Indian Journal of Experimental Biology. - New Delhi, India : National Institute of Science Communication and Information Resources. - 0019-5189 .- 0975-1009. ; 57, s. 825-838
-
Journal article (peer-reviewed)abstract
- Fresh vegetables are potential source of lactic acid bacteria (LAB). In the present study, LAB were isolated from the fresh vegetables from Pune region. Total 266 LAB were isolated from the edible parts of fresh vegetables viz. cauliflower, gherkins, cluster beans, fenugreek, cow pea, bitter gourd, french beans, tomato, ridged gourd, cucumber and bottle gourd. On phenotypic and molecular characterization predominant genera obtained were Lactobacillus, Enterococcus and Weissella. Twenty one isolates exhibited tolerance to bile salt, acidic pH and pancreatin. Cellular extracts of several isolates with ability to survive in artificial intestinal condition additionally showed antioxidant potential and cell free supernatants xhibited antibacterial potential against selected plant and human pathogens. Bacteriocin and bacteriocin like substances (BLS) substances secreted by these isolates can be used for food preservation.
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| 4. |
- Junnarkara, Manisha V., et al.
(author)
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Evaluation of Probiotic Potential of Lactic Acid Bacteria Isolated from Different Sources in Western India
- 2018
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In: Food biotechnology. - United Kingdom : Taylor & Francis. - 0890-5436 .- 1532-4249. ; 32:2, s. 112-129
-
Journal article (peer-reviewed)abstract
- Lactic acid bacteria isolated from unconventional sources are often attractive targets in the quest for obtaining better probiotics. In the present study, 16 members of the genus Lactobacillus, isolated from 3 different sources in western India, viz., plants, fermented foods and beverages, and human feces, were evaluated for their probiotic and bioactive properties. The isolates were closely related to Lactobacillus fermentum, Lactobacillus pentosus, and mainly Lactobacillus plantarum. The isolates were tolerant to bile salt, acidic pH and pancreatin, although pancreatin tolerance was generally low. Cellular extracts of several isolates displayed antioxidant activity, while cell-free supernatants displayed antibacterial activity against human pathogens. Antioxidant activity of Lactobacilli of human origin was higher than those from vegetables or fermented foods and beverages. L. plantarum AG40V prevented spoilage of fresh-cut fruits, vegetables and sprouted mung-beans. Lactobacilli from all sources displayed equal probiotic potential and those of human origin displayed superior antioxidant activity over others.
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| 5. |
- Nahar, Noor, et al.
(author)
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Functional studies of AtACR2 gene putatively involved in accumulation, reduction and/or sequestration of arsenic species in plants
- 2017
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In: Biologia. - : Walter de Gruyter. - 0006-3088 .- 1336-9563. ; 72:5, s. 520-526
-
Journal article (peer-reviewed)abstract
- Food-based exposure to arsenic is a human carcinogen and can severely impact human health resulting in many cancerous diseases and various neurological and vascular disorders. This project is a part of our attempts to develop new varieties of crops for avoiding arsenic contaminated foods. For this purpose, we have previously identified four key genes, and molecular functions of two of these, AtACR2 and AtPCSl, have been studied based on both in silico and in vivo experiments. In the present study, a T-DNA tagged mutant, (SALK-143282C with mutation in AtACR2 gene) of Arabidopsis thaliana was studied for further verification of the function of AtACR2 gene. Semi-quantitative RT-PCR analyses revealed that this mutant exhibits a significantly reduced expression of the AtACR2 gene. When exposed to 100 μM of arsenate (AsV) for three weeks, the mutant plants accumulated arsenic approximately three times higher (778 μg/g d. wt.) than that observed in the control plants (235 μg/g d. wt.). In contrast, when the plants were exposed to 100 μM of arsenite (AsIII), no significant difference in arsenic accumulation was observed between the control and the mutant plants (535 μg/g d. wt. and 498 μg/g d. wt., respectively). Also, when arsenate and arsenite was measured separately either in shoots or roots, significant differences in accumulation of these substances were observed between the mutant and the control plants. These results suggest that AtACR2 gene is involved not only in accumulation of arsenic in plants, but also in conversion of arsenate to arsenite inside the plant cells. © 2017 Institute of Molecular Biology, Slovak Academy of Sciences.
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| 6. |
- Nahar, Noor, et al.
(author)
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In silico and in vivo studies of molecular structures and mechanisms of AtPCS1 protein involved in binding arsenite and/or cadmium in plant cells
- 2014
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In: Journal of Molecular Modeling. - : Springer. - 1610-2940 .- 0948-5023. ; 20:3
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Journal article (peer-reviewed)abstract
- This paper reports a continuation of our previous research on the phytochelatin synthase1 (PCS1) gene involved in binding and sequestration of heavy metals or metalloids in plant cells. Construction of a 3D structure of the Arabidopsis thaliana PCS1 protein and prediction of gene function by employing iterative implementation of the threading assembly refinement (I-TASSER) revealed that PC ligands (3GC-gamma-glutamylcysteine) and Gln50, Pro53, Ala54, Tyr55, Cys56, Ile102, Gly161, His162, Phe163, Asp204 and Arg211 residues are essential for formation of chelating complex with cadmium (Cd²⁺) or arsenite (AsIII). This finding suggests that the PCS1 protein might be involved in the production of the enzyme phytochelatin synthase, which might in turn bind, localize, store or sequester heavy metals in plant cells. For validation of the in silico results, we included a T-DNA tagged mutant of Arabidopsis thaliana, SAIL_650_C12, (mutation in AtPCS1 gene) in our investigation. Furthermore, using reverse transcriptase PCR we confirmed that the mutant does not express the AtPCS1 gene. Mutant plants of SAIL_650_C12 were exposed to various amounts of cadmium (Cd²⁺) and arsenite (AsIII) and the accumulation of these toxic metals in the plant cells was quantified spectrophotometrically. The levels of Cd²⁺ and AsIII accumulation in the mutant were approximately 2.8 and 1.6 times higher, respectively, than that observed in the wild-type controlled plants. We confirmed that the results obtained in in silico analyses complement those obtained in in vivo experiments.
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| 7. |
- Nahar, Noor, et al.
(author)
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Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana
- 2017
-
In: Journal of plant physiology (Print). - : Elsevier. - 0176-1617 .- 1618-1328. ; 218, s. 121-126
-
Journal article (peer-reviewed)abstract
- We have cloned, characterized and transformed the AtACR2 gene (arsenic reductase 2) of Arabidopsis thaliana into the genome of tobacco (Nicotiana tabacum, var Sumsun). Our results revealed that the transgenic tobacco plants are more tolerant to arsenic than the wild type ones. These plants can grow on culture medium containing 200μM arsenate, whereas the wild type can barely survive under this condition. Furthermore, when exposed to 100μM arsenate for 35days the amount of arsenic accumulated in the shoots of transgenic plants was significantly lower (28μg/g d wt.) than that found in the shoots of non-transgenic controls (40μg/g d wt.). However, the arsenic content in the roots of transgenic plants was significantly higher (2400μg/g d. wt.) than that (2100μg/g d. wt.) observed in roots of wild type plants. We have demonstrated that Arabidopsis thaliana AtACR2 gene is a potential candidate for genetic engineering of plants to develop new crop cultivars that can be grown on arsenic contaminated fields to reduce arsenic content of the soil and can become a source of food containing no arsenic or exhibiting substantially reduced amount of this metalloid.
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| 8. |
- Nawani, Neelu, et al.
(author)
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Actinomycetes : Role in Biotechnology and Medicine
- 2013
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In: BioMed Research International. - New York, USA : Hindawi Publishing Corporation. - 2314-6133 .- 2314-6141. ; :2013, s. 687190-
-
Journal article (peer-reviewed)abstract
- Actinomycetes, one of the most diverse groups of filamentous bacteria, are well recognized for their metabolic versatility. The bioactive potential of these bacteria facilitates their survival even in distress and unfavourable ecological conditions. This special issue is dedicated to the importance of multitude of primary and secondary metabolites produced by actinomycetes. The importance of large repertory of enzymes from actinomycetes and their potential in replacing chemical catalysts is discussed.
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| 9. |
- Nawani, Neelu, et al.
(author)
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Microbial biomass for sustainable remediation of wastewater
- 2022
-
In: Biomass, Biofuels, Biochemicals. - : Elsevier. - 9780323885119 ; , s. 271-292
-
Book chapter (peer-reviewed)abstract
- Microbial technologies play an extremely crucial role in wastewater treatment. In the last few decades, the focus was on wastewater treatment; however, with the consumption of already finite resources, this focus has now shifted to wastewater remediation and recycling. The latest technologies are more robust at wastewater treatment and the recovery of useful compounds from the wastewaters. This chapter compiles the latest technologies in which microbial/bacterial biomass was used for wastewater treatment and remediation. This chapter also gives a special focus on remediation of wastewaters contaminated with heavy metals, describing the basic technologies as well as smart technologies. Emphasis is given to circular bioeconomy and how it can be implemented in wastewater treatment. A circular model incorporating key areas such as point source detection of pollutants, characterization of wastewaters, choice of the remedial technology, and type of treatment is explained in the chapter.
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| 10. |
- Nawani, Neelu, et al.
(author)
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Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora
- 2016
-
In: Biologia. - Bratislava : Springer. - 0006-3088 .- 1336-9563. ; 71:5, s. 494-507
-
Journal article (peer-reviewed)abstract
- This study illustrates the sporadic distribution of metals in fluvial systems flowing from catchments to urban settlements. This is a detailed study prognosticating the deteriorating quality of rivers at specific locations due to metal pollution. Heavy metals like cadmium, lead, nickel and mercury are prominent in industrial sector. Contour plots derived using spatial and temporal data could determine the focal point of metal pollution and its gradation. Metal values recorded were cadmium 157 mg/L, lead 47 mg/L, nickel 61 mg/L and mercury 0.56 mg/L. Prokaryote diversity was less in polluted water and it harboured metal tolerant bacteria, which were isolated from these polluted sites. Actinomycetes like Streptomyces and several other bacteria like Stenotrophomonas and Pseudomonas isolated from the polluted river sites exhibited changes in morphology in presence of heavy metals. This stress response offered remedial measures as Streptomyces were effective in biosorption of cadmium, nickel and lead and Stenotrophomonas and Pseudomonas were effective in the bioaccumulation of lead and cadmium. The amount of 89 mg of lead and 106 mg of nickel could be adsorbed on one gram of Streptomyces biomass-based biosorbent. Such biological remedies can be further explored to remove metals from polluted sites and from metal contaminated industrial or waste waters.
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| 11. |
- Prakash, Divya, et al.
(author)
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Actinomycetes : A Repertory of Green Catalysts with a Potential Revenue Resource
- 2013
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In: BioMed Research International. - : Hindawi Publishing Corporation. - 2314-6133 .- 2314-6141.
-
Research review (peer-reviewed)abstract
- Biocatalysis, one of the oldest technologies, is becoming a favorable alternative to chemical processes and a vital part of green technology. It is an important revenue generating industry due to a global market projected at $7 billion in 2013 with a growth of 6.7% for enzymes alone. Some microbes are important sources of enzymes and are preferred over sources of plant and animal origin. As a result, more than 50% of the industrial enzymes are obtained from bacteria. The constant search for novel enzymes with robust characteristics has led to improvisations in the industrial processes, which is the key for profit growth. Actinomycetes constitute a significant component of the microbial population in most soils and can produce extracellular enzymes which can decompose various materials. Their enzymes are more attractive than enzymes from other sources because of their high stability and unusual substrate specificity. Actinomycetes found in extreme habitats produce novel enzymes with huge commercial potential. This review attempts to highlight the global importance of enzymes and extends to signify actinomycetes as promising harbingers of green technology.
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| 12. |
- Prakash, Mansi, et al.
(author)
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Diverse pathological implications of YKL-40 : Answers may lie in 'outside-in' signaling
- 2013
-
In: Cellular Signalling. - : Elsevier. - 0898-6568 .- 1873-3913. ; 25:7, s. 1567-1573
-
Research review (peer-reviewed)abstract
- The developing paradigms about YKL-40, a member of the "mammalian chitinase-like proteins", from across the globe, project it as a vital parameter for the detection of disease onset and progression. It is expressed and secreted by cancer cells of different origins along with a variety of non-malignant cells including inflammatory and structural cells. Numerous studies demonstrate that YKL-40 over-expression is associated with increased patient mortality though the cellular receptors responsible for mediating these effects have not yet been identified. The putative YKL-40 ligands are thought to be carbohydrate structures, since it is capable of binding chitin, chito-oligosaccharides and heparin. Binding of collagen to YKL-40, identified it as the only non-carbohydrate extracellular matrix (ECM) ligand for YKL-40. Our broad understanding of YKL-40 as a versatile biomarker and its involvement in activating several signaling pathways make us anticipate that its specific receptors/binding partners may exist on the cell surface also. The cell surface heparan sulfate (HS) moieties seem to be the potential candidates for this role, suggesting that it could interact with HS-proteoglycans. It is recommended to clearly delineate YKL-40-mediated signaling mechanisms before promoting the YKL-40 know-how for translational research, in both diagnostic and therapeutic applications. The present review provides an overview of YKL-40 as a versatile biomarker, discussing the related pathological mechanisms and aims to reassess and unify the already proposed diverse hypotheses in YKL-40-regulated signaling mechanisms. (c) 2013 Elsevier Inc. All rights reserved.
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| 13. |
- Rahman, Aminur, 1984-, et al.
(author)
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Bioremediation of hexavalent chromium (VI) by a soil-borne bacterium, Enterobacter cloacae B2-DHA
- 2015
-
In: Journal of Environmental Science and Health. Part A. - : Informa UK Limited. - 1093-4529 .- 1532-4117. ; 50:11, s. 1136-1147
-
Journal article (peer-reviewed)abstract
- Chromium and chromium containing compounds are discharged into the nature as waste from anthropogenic activities, such as industries, agriculture, forest farming, mining and metallurgy. Continued disposal of these compounds to the environment leads to development of various lethal diseases in both humans and animals. In this paper, we report a soil borne bacterium, B2-DHA that can be used as a vehicle to effectively remove chromium from the contaminated sources. B2-DHA is resistant to chromium with a MIC value of 1000 mu g mL(-1) potassium chromate. The bacterium has been identified as a Gram negative, Enterobacter cloacae based on biochemical characteristics and 16S rRNA gene analysis. TOF-SIMS and ICP-MS analyses confirmed intracellular accumulation of chromium and thus its removal from the contaminated liquid medium. Chromium accumulation in cells was 320 mu g/g of cells dry biomass after 120-h exposure, and thus it reduced the chromium concentration in the liquid medium by as much as 81%. Environmental scanning electron micrograph revealed the effect of metals on cellular morphology of the isolates. Altogether, our results indicate that B2-DHA has the potential to reduce chromium significantly to safe levels from the contaminated environments and suggest the potential use of this bacterium in reducing human exposure to chromium, hence avoiding poisoning.
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| 14. |
- Rahman, Aminur, 1984-, et al.
(author)
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Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
- 2015
-
In: Genomics. - : Elsevier. - 0888-7543 .- 1089-8646. ; 106:6, s. 384-392
-
Journal article (peer-reviewed)abstract
- Previously, we reported an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, isolated from an arsenic contaminated lands. Here, we have investigated its genetic composition and evolutionary history by using massively parallel sequencing and comparative analysis with other known Lysinibacillus genomes. Assembly of the sequencing reads revealed a genome of ~ 4.5 Mb in size encompassing ~ 80% of the chromosomal DNA. We found that the set of ordered contigs contains abundant regions of similarity with other Lysinibacillus genomes and clearly identifiable genome rearrangements. Furthermore, all genes of B1-CDA that were predicted be involved in its resistance to arsenic and/or other heavy metals were annotated. The presence of arsenic responsive genes was verified by PCR in vitro conditions. The findings of this study highlight the significance of this bacterium in removing arsenics and other toxic metals from the contaminated sources. The genetic mechanisms of the isolate could be used to cope with arsenic toxicity.
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| 15. |
- Rahman, Aminur, 1984-, et al.
(author)
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Data in support of the comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
- 2015
-
In: Data in Brief. - : Elsevier. - 2352-3409. ; 5, s. 579-585
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Journal article (peer-reviewed)abstract
- This study is a part of our long term project on bioremediation of toxic metals and other pollutants for protection of human health and the environment from severe contamination. The information and results presented in this data article are based on both in vitro and in silico experiments. In vitro experiments were used to investigate the presence of arsenic responsive genes in a bacterial strain B1-CDA that is highly resistant to arsenics. However, in silico studies were used to annotate the function of the metal responsive genes. By using this combined study consisting of in vitro and in silico experiments we have identified and characterized specific genes from B1-CDA that can be used as a potential tool for removal of arsenics as well as other heavy metals from the contaminated environment.
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| 16. |
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| 17. |
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| 18. |
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| 19. |
- Rahman, Aminur, et al.
(author)
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Genome Sequencing Revealed Chromium and Other Heavy Metal Resistance Genes in E. cloacae B2-Dha
- 2017
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In: Journal of Microbial & Biochemical Technology. - : Omics Publishing Group. - 1948-5948. ; 9:5, s. 191-199
-
Journal article (peer-reviewed)abstract
- The previously described chromium resistant bacterium, Enterobacter cloacae B2-DHA, was isolated from leather manufacturing tannery landfill in Bangladesh. Here we report the entire genome sequence of this bacterium containing chromium and other heavy metal resistance genes. The genome size and the number of genes, determined by massive parallel sequencing and comparative analysis with other known Enterobacter genomes, are predicted to be 4.22 Mb and 3958, respectively. Nearly 160 of these genes were found to be involved in binding, transport, and catabolism of ions as well as efflux of inorganic and organic compounds. Specifically, the presence of two chromium resistance genes, chrR and chrA was verified by polymerase chain reaction. The outcome of this research highlights the significance of this bacterium in bioremediation of chromium and other toxic metals from the contaminated sources.
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| 20. |
- Rahman, Aminur, et al.
(author)
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Investigation on Arsenic-Accumulating and Arsenic-Transforming Bacteria for Potential Use in the Bioremediation of Arsenics
- 2017
-
In: Handbook of Metal-Microbe Interactions and Bioremediation. - Boca Raton, FL : CRC Press. - 9781498762427 - 9781498762434 ; , s. 509-520
-
Book chapter (peer-reviewed)abstract
- In this chapter, arsenic-accumulating and arsenic- transformingbacterial strains that can be employed as a sourcefor cost-effective and eco-friendly bioremediation of arsenicsfrom contaminated environments have been reviewed. Thischapter demonstrates that many naturally occurring bacterialstrains like B1-CDA have the potential for reducing arseniccontent in contaminated sources to safe levels. Therefore,the socioeconomic impact of this kind of microorganisms ishighly significant for those countries, especially in the developingworld, where impoverished families and villages aremost impacted. Therefore, this discovery should be consideredto be the most significant factor in formulating nationalstrategies for effective poverty elimination. Besides humanarsenic contamination, these bacterial strains will also benefitlivestock and native animal species, and the outcome ofthese studies is vital not only for people in arsenic-affectedareas but also for human populations in other countries thathave credible health concerns as a consequence of arseniccontaminatedwater and foods.
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| 21. |
- Rahman, Aminur, 1984-, et al.
(author)
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Isolation and characterization of a Lysinibacillus strain B1-CDA showing potential for bioremediation of arsenics from contaminated water
- 2014
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In: Journal of Environmental Science and Health. Part A. - : Taylor & Francis. - 1093-4529 .- 1532-4117. ; 49:12, s. 1349-1360
-
Journal article (peer-reviewed)abstract
- The main objective of this study was to identify and isolate arsenic resistant bacteria that can be used for removing arsenic from thecontaminated environment. Here we report a soil borne bacterium, B1-CDA that can serve this purpose. B1-CDA was isolated fromthe soil of a cultivated land in Chuadanga district located in the southwest region of Bangladesh. The morphological, biochemicaland 16S rRNA analysis suggested that the isolate belongs to Lysinibacillus sphaericus. The minimum inhibitory concentration (MIC)value of the isolate is 500 mM (As) as arsenate. TOF-SIMS and ICP-MS analysis confirmed intracellular accumulation and removalof arsenics. Arsenic accumulation in cells amounted to 5.0 mg g¡1 of the cells dry biomass and thus reduced the arsenicconcentration in the contaminated liquid medium by as much as 50%. These results indicate that B1-CDA has the potential forremediation of arsenic from the contaminated water. We believe the benefits of implementing this bacterium to efficiently reducearsenic exposure will not only help to remove one aspect of human arsenic poisoning but will also benefit livestock and native animalspecies. Therefore, the outcome of this research will be highly significant for people in the affected area and also for humanpopulations in other countries that have credible health concerns as a consequence of arsenic-contaminated water.
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| 22. |
- Salgaonkar, Neeta A., et al.
(author)
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Use of N,N-diacetylchitobiose in decreasing toxic effects of indoor air pollution by preventing oxidative DNA damage
- 2016
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In: Biologia. - Bratislava : Springer. - 0006-3088 .- 1336-9563. ; 71:5, s. 505-515
-
Journal article (peer-reviewed)abstract
- Indoor air pollution occurs due to hazardous pollutants, such as tobacco smoke, pesticides and carbon oxides, sulphur oxides and nitrogen oxides arising from combustion of biomass fuels. Exposure to these pollutants results in respiratory conditions like asthma, chronic obstructive pulmonary disease, lung cancer, pneumonia and other lower respiratory infections. Several of these infections are a result of inflammation and oxidative stress. Here we demonstrate the ability of N,N-diacetylchitobiose in preventing oxidative DNA damage in peripheral blood mononuclear cells exposed to biomass smoke extracts and cigarette smoke extract. The cytotoxic effect of these pollutants was determined by trypan blue exclusion assay in peripheral blood mononuclear cells, where cytotoxicity in decreasing order was garette > wood > sawdust > cowdung. Cytotoxicity could be due to single- and double-strand breaks in the DNA as a result of oxidative stress. Comet assay measures the extent of DNA damage in the cells exposed to toxic agents. When mononuclear cells were treated with N,N-diacetylchitobiose and later exposed to smoke extracts, the extent of DNA damage decreased by 44.5% and 57.5% as compared to untreated cells. The protection offered by N,N-diacetylchitobiose towards oxidative DNA damage was at par with quercetin, a popular herbal medicine. Glutathione-S-transferase activity was determined in mononuclear cells exposed to smoke extracts, where oxidative stress in cells exposed to cigarette smoke extract was maximum. The present study demonstrates for the first time the ability of N,N -diacetylchitobiose to alleviate the harmful effects of indoor air pollutants.
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| 23. |
- Yewale, Priti Prabhakar, et al.
(author)
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Molecular profiling of multidrug-resistant river water isolates : insights into resistance mechanism and potential inhibitors
- 2020
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In: Environmental Science and Pollution Research. - : Springer. - 0944-1344 .- 1614-7499. ; 27:22, s. 27279-27292
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Journal article (peer-reviewed)abstract
- Polluted waters are an important reservoir for antibiotic resistance genes and multidrug-resistant bacteria. This report describes the microbial community, antibiotic resistance genes, and the genetic profile of extended spectrum β-lactamase strains isolated from rivers at, Pune, India. ESBL-producing bacteria isolated from diverse river water catchments running through Pune City were characterized for their antibiotic resistance. The microbial community and types of genes which confer antibiotic resistance were identified followed by the isolation of antibiotic-resistant bacteria on selective media and their genome analysis. Four representative isolates were sequenced using next generation sequencing for genomic analysis. They were identified as Pseudomonas aeruginosa, Escherichia coli, and two isolates were Enterobacter cloacae. The genes associated with the multidrug efflux pumps, such as tolC, macA, macB, adeL, and rosB, were detected in the isolates. As MacAB-TolC is an ABC type efflux pump responsible for conferring resistance in bacteria to several antibiotics, potential efflux pump inhibitors were identified by molecular docking. The homology model of their MacB protein with that from Escherichia coli K12 demonstrated structural changes in different motifs of MacB. Molecular docking of reported efflux pump inhibitors revealed the highest binding affinity of compound MC207-110 against MacB. It also details the potential efflux pump inhibitors that can serve as possible drug targets in drug development and discovery.
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| 24. |
- Yewale, Priti Prabhakar, et al.
(author)
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Sources of Metal Pollution, Global Status, and Conventional Bioremediation Practices
- 2017
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In: Handbook of Metal–Microbe Interactions and Bioremediation. - Boca Raton, FL : CRC Press. - 9781498762427 - 9781498762434 ; , s. 25-40
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Book chapter (peer-reviewed)abstract
- Pollution control has become a priority task for global regulatory authorities. The framing of regulations, guidelines, and implementation of pollution awareness and control programs has begun at a massive scale. Heavy metals that are one of the most challenging pollutants that affect humans, animals, plants, and the ecosystem health. The sources of different metals and their toxicities are described. Current approaches in bioremediation are addressed along with the challenges posed by them. Furthermore, recent developments in biotechnology that offer novel ways to recover metals from contaminated sites are discussed.
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| 25. |
- Yewale, Priti, et al.
(author)
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Studies on Biosmotrap : A multipurpose biological air purifier to minimize indoor and outdoor air pollution
- 2022
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In: Journal of Cleaner Production. - : Elsevier. - 0959-6526 .- 1879-1786. ; 357
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Journal article (peer-reviewed)abstract
- Air pollution is a serious health concern that affects many people across the globe. The major air pollutants are particulate matter, carbon oxides, nitrogen oxides, sulphur oxides, volatile organic compounds, polycyclic aromatics and free radicals which cause severe respiratory distress and infections. The existing air cleaning systems suffer from drawbacks of high cost and generation of secondary pollutants. A novel biological air filter “Biosmotrap” which is a laminate composite of sponge gourd and algae was developed. Biosmotrap placed in a carrier assembly on exhaust of vehicles, could remove carbon monoxide, nitric oxide, nitrogen dioxide, and fine particulate matter (PM2.5) from the vehicular emissions resulting in cleaner emissions. Biosmotrap decreased carbon monoxide from 1,423,992 μg/m3 to 76,756 μg/m3, nitric oxide from 71,128 μg/m3 to 9982 μg/m3, nitrogen dioxide from 565 μg/m3 to 188 μg/m3 and PM2.5 from 3200 μg/m3 to 60 μg/m3 from a polluting vehicle. Biosmotrap removed 60–80% of indoor pollutants from cigarette smoke and incense-stick smoke. Biosmotrap could protect the human cells from oxidative DNA damage induced by indoor air pollutants. Hibiscus rosa-sinensis plants exposed to air filtered through Biosmotrap were healthy as compared to the plants directly exposed to polluted air. Biosmotrap is an economic, efficient, eco-friendly filter that is superior to existing air filtration methods.
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