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Träfflista för sökning "WFRF:(Nijenhuis S.) "

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  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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  • Ortiz Catalan, Max Jair, 1982, et al. (author)
  • Virtual Reality
  • 2014
  • In: Biosystems and Biorobotics. - Berlin, Heidelberg : Springer Berlin Heidelberg. - 2195-3570 .- 2195-3562. - 9783642385551 ; 4, s. 249-265
  • Book chapter (other academic/artistic)abstract
    • This chapter provides an overview on the use of Virtual Reality (VR) in rehabilitation with respect to recent neuroscience and physical therapy reviews of individuals with motor impairments. A wide range of technologies have been employed to provide rehabilitation supported by VR. Several studies have found evidence of the benefits of VR rehabilitation technologies. However, support for their efficacy is still limited due the lack of generalizable results and the uncoordinated effort of many individual, heterogeneous studies that have been conducted. Although VR has clear potential as a rehabilitation tool to improve treatment outcomes, future trials need to take into account the individual perspective of each patient group and consolidate research methodologies across trials to allow for stronger conclusions across the heterogeneous field of neurorehabilitation. Interventions must be designed with a strong focus on the patient’s needs and clinical outcomes, rather than on the technology available to the clinician.
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  • Result 1-5 of 5

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