| 1. |
- Amelia, Tudoran, et al.
(författare)
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A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera- than Coleoptera-targeted Bacillus thuringiensis strains
- 2021
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Ingår i: Pest Management Science. - : Wiley. - 1526-498X .- 1526-4998. ; 77, s. 1303-1315
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND The pine weevil (Hylobius abietis) is a major forest regeneration pest causing high levels of seedling mortality and economic losses. Current management relies on silviculture, stem coatings and insecticides. Here we evaluated for the first time the effects of Bacillus thuringiensis (Bt) strains on H. abietis adults: two producing the Coleoptera-targeted toxins Cry3Aa (Bt tenebrionis NB-176) and Cry8Da (Bt galleriae SDS-502), and one producing the Diptera-targeted Cry10A (Bt israelensis AM65-52). Choice and nonchoice assays using individual and mixtures of Bt formulations, containing these strains respectively, were conducted.RESULTS We found that Bt had toxic and lethal effects on H. abietis, but effects varied with strain and formulation concentration. The Diptera-targeted Bt israelensis had the most negative effects on weevil weight, feeding and mortality (70-82% feeding reduction, 65-82% greater mortality than control), whereas the effect was lower for the Coleoptera-specific Bt tenebrionis (38-42%; 37-42%) and Bt galleriae (11-30%; 15-32%). Reduced weevil feeding was observed after 3 days, and the highest mortality occurred 7-14 days following Bt exposure. However, we found no synergistic toxic effects, and no formulation combination was better than Bt israelensis alone at reducing consumption and survival. Also, pine weevils were not deterred by Bt, feeding equally on Bt-treated and non-Bt treated food.CONCLUSION There is potential to develop forest pest management measures against H. abietis that include Bt, but only the Diptera-targeted Bt israelensis would provide effective seedling protection. Its Diptera-specificity may need reconsideration, and evaluation of other Bt strains/toxins against H. abietis would be of interest.
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| 2. |
- Attorps, Iiris, et al.
(författare)
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MST education in Swedish preschool
- 2013
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Konferensbidrag (refereegranskat)abstract
- The present study is part of the EU-Fp7 project SECURE. The research question in the present study is: How is the current Swedish MST curriculum for preschool implemented by the teachers, and perceived by the 5-year old learners?The introduction of a revised preschool curriculum in 2011 has given MST a prominent place in Swedish preschool, and may be intended by the authorities to change the weak positions and declining trends in the PISA and TIMSS surveys for Swedish school children. The new teaching task seems to be highly appreciated by the preschool teachers. However, the curriculum is quite ambitious, and a major concern is how the implementation will be done. The concept of ”emergent science” is discussed in order to induce positive attitudes towards MST.In this study, semi-structured interviews with teachers in 15 Swedish preschools are the main source of information combined with some interviews with learners and photographic materials. The method for the analysis uses a category system of which results from some of the categories are reported.The preschool teachers are aware of the new role as teachers rather than just child minders. The revised curriculum seems to increase the self-confidence for MST of the learners as well as the teachers. The teachers say that the learners are interested in MST and that it is a case of catching opportunities, which may lead to emergent science. The 5-year old learners confirm that they like the activities related to MST.Working with hypotheses and storyline activities is shown as an example how emergent science can be promoted in 5-year old children. Activities connected to ”emerging science” have been common in Swedish preschools for some time, but after the implementation of the revised curriculum teachers also verbalize this for themselves and the learners.
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| 3. |
- Attorps, Iiris, et al.
(författare)
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MST-utbildning i den svenska förskolan
- 2013
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Konferensbidrag (refereegranskat)abstract
- Svenska elever hamnar efter i PISA- och TIMSS-undersökningarna. I matematik har matningarna visat på sjunkande kunskaper sedan början av 2000-talet och i naturvetenskap ligger de svenska eleverna ständigt under det internationella genomsnittet. Detta är alarmerande för landet och skälen bör utredas.Som en del i EU-projektet SECURE inom ramprogrammet Fp7 presenteras resultat från lokal forskning om genomförandet av MST-kursplanerna och attityder till ämnena bland lärare och elever. Med MST avser vi matematik, naturvetenskap och teknik. Intervjuer och enkäter har utförts bland lärare och elever i 47 klasser i 15 skolenheter i några mellansvenska kommuner. De åldersgrupper som studerats är elever från åldersgrupperna 5, 8, 11 resp. 13 år. Vi fokuserar här på förskolans verksamhet, d.v.s. arbetet med 5-åringar.Som en följd av införandet 2011 av en reviderad läroplan för förskolan, kan vi se att MST har fått en framträdande plats i den svenska förskolan som verkar vara mycket uppskattad av förskollärarna. I den reviderade läroplanen framgår att förskollärare ska ansvara för att arbetet i barngruppen genomförs så att barnen stimuleras och utmanas i sin matematiska utveckling, samt stimuleras och utmanas i sitt intresse för naturvetenskap och teknik. Exempel på hur de skall gå tillväga finns också i läroplanen. Detta kan i praktiken uppfattas som en informell tidigareläggning av den obligatoriska skolan i Sverige, eftersom förskolan inte är obligatorisk men utnyttjas av de allra flesta. Det skulle därmed kunna innebära en vändpunkt för resultaten i grundskolan och förhoppningsvis ge avtryck i kommande PISA- och TIMSS-undersökningar.De förskollärare som intervjuats är medvetna om vikten av att undervisa MST i förskolan. Detta tydliggörs av några uttalanden från intervjuade förskollärare. 'Det som är nytt nu är att man pratar om lärare och att vi utbildar barnen.' 'Det här lärandet är för livet. Det är inte bara för individen. Verksamheten på förskolan bidrar till hela samhällsutvecklingen.' Även föräldrarnas förväntningar på förskolan har förändrats. 'Föräldrarna uppskattar resultaten i matematik och ser förskolan som en skolform snarare än förvaring av barn.'I denna presentation ges exempel på hur MST-ämnena undervisas på ett integrerat sätt i förhållande till den praxis som råder i svenska förskolan, samt i förhållande till ålder och mognad hos barnen.
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| 4. |
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| 5. |
- Axelsson, Karolin, et al.
(författare)
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Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis
- 2017
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Ingår i: Microbial Ecology. - : SPRINGER. - 0095-3628 .- 1432-184X. ; 74:1, s. 177-184
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Tidskriftsartikel (refereegranskat)abstract
- The pine weevil, Hylobius abietis, is a severe forest pest insect as it feeds on newly planted conifer seedlings. To identify and develop an antifeedant could be one step towards the protection of seedlings from feeding damage by the pine weevil. With the aim to trace the origin of the antifeedants previously found in feces of the pine weevil, we investigated the culturable bacteria associated with the gut and identified the volatiles they produced. Bacterial isolates were identified by 16S ribosomal RNA gene analysis. The volatile emissions of selected bacteria, cultivated on NB media or on the grated phloem of Scots pine twigs dispersed in water, were collected and analyzed by solid-phase microextraction gas chromatography-mass spectrometry. The bacterial isolates released a variety of compounds, among others 2-methoxyphenol, 2-phenylethanol, 3-methyl-1-butanol, 1-octen-3-ol, 3-octanone, dimethyl disulfide, and dimethyl trisulfide. A strong antifeedant effect was observed by 2-phenylethanol, which could thus be a good candidate for use to protect planted conifer seedlings against feeding damage caused by H. abietis.
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| 6. |
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| 7. |
- Azeem, Muhammad, et al.
(författare)
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A fungal metabolite masks the host plant odor for the pine weevil (Hylobius abietis)
- 2015
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Ingår i: Fungal ecology. - : Elsevier BV. - 1754-5048 .- 1878-0083. ; 13, s. 103-111
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Tidskriftsartikel (refereegranskat)abstract
- The pine weevil is one of the most important pest insects of conifer reforestation areas in Europe. Female pine weevils cover their eggs with chewed bark and feces (frass) resulting in avoidance behavior of feeding conspecifics towards egg laying sites. It has been suggested that microorganisms present in the frass may be responsible for producing deterrent compounds for the pine weevil. The fungi Ophiostoma canum, O. pluriannulatum, and yeast Debaryomyces hansenii were isolated from aseptically collected pine-weevil frass. The isolated fungi were cultured on weevil frass broth and their volatiles were collected by SPME and identified by GC MS. D. hansenii produced methyl salicylate (MeS) as a major compound, whereas, in addition, O. canum and O. pluriannulatum produced 6-protoilludene. In a multi-choice lab bioassay, MeS strongly reduced pine weevil's attraction to the Pinus sylvestris volatiles. Thus, a fungal metabolite was found that strongly affects the pine weevil host-odor search. (C) 2014 Elsevier Ltd and The British Mycological Society. All rights reserved.
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| 8. |
- Azeem, Muhammad, et al.
(författare)
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Chemical composition and antifeedant activity of some aromatic plants against pine weevil (Hylobius abietis)
- 2020
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Ingår i: Annals of Applied Biology. - : Blackwell Publishing Ltd. - 0003-4746 .- 1744-7348.
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Tidskriftsartikel (refereegranskat)abstract
- The pine weevil Hylobius abietis is an important pest causing severe damage to conifer seedlings in reforestation areas in Europe and Asia. Plants that have no evolutionary history with the pine weevil are of special interest in the search for compounds with a strong antifeedant activity. Thus, the essential oils of nine aromatic plants, viz Amomum subulatum, Cinnamomum tamala, Curcuma longa, Laurus nobilis, Ocimum basilicum, Origanum majorana, Origanum vulgare, Syzygium aromaticum and Trachyspermum ammi were extracted by hydrodistillation. The essential oil constituents were identified by gas chromatography–mass spectrometry, and antifeedant properties towards the pine weevil were assessed using choice feeding bioassay. The essential oils of C. longa, O. majorana, S. aromaticum and T. ammi showed an excellent antifeedant activity towards the pine weevil for 24 hr, whereas the essential oil of other plants showed the activity for 6 hr. There was a positive correlation between the amount of benzenoid compounds and the antifeedant activity of the essential oils. This study suggests that pine weevil non-host plant compounds have potential to be used for the protection of seedlings against pine weevil feeding. However, further study will be needed to explore the antifeedant activity of individual components and oils in the laboratory as well as in the field.
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| 9. |
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| 10. |
- Azeem, Muhammad, et al.
(författare)
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Chemodiversity and biodiversity of fungi associated with the pine weevil Hylobius abietis
- 2015
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Ingår i: Fungal Biology. - : Elsevier BV. - 1878-6146 .- 1878-6162. ; 119:8, s. 738-46
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Tidskriftsartikel (refereegranskat)abstract
- The pine weevil Hylobius abietis is a severe pest of conifer seedlings in reforestation areas. Weevils lay eggs in the root bark or in the soil near roots of recently dead trees and cover the eggs with frass (feces combined with chewed bark), possibly to avoid conspecific egg predation. The aim of the present investigation focused on isolation, identification, and volatile production of fungi from pine-weevil feces and frass. Fungi were isolated from weevil frass and feces separately, followed by identification based on ITS sequencing. Fifty-nine isolates belonging to the genera Penicillium, Ophiostoma, Mucor, Leptographium, Eucasphaeria, Rhizosphaera, Debaryomyces, and Candida were identified. Volatile organic compounds (VOCs) produced by the fungal community and fungal isolates cultured on weevil-frass broth were identified by SPME-GCMS. Major VOCs emitted from the fungal community and pure isolates were species- and strain specific and included isopentylalcohol, styrene, 3-octanone, 6-protoilludene, methyl salicylate, 3-methylanisole, 2-methoxyphenol, and phenol. Some of these are known to influence the orientation of pine weevils when tested among highly attractive newly planted conifer seedlings.
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| 11. |
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| 12. |
- Azeem, Muhammad, et al.
(författare)
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Penicillium expansum Volatiles Reduce Pine Weevil Attraction to Host Plants
- 2013
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Ingår i: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 39:1, s. 120-128
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Tidskriftsartikel (refereegranskat)abstract
- The pine weevil Hylobius abietis (L.) is a severe pest of conifer seedlings in reforested areas of Europe and Asia. To identify minimally toxic and ecologically sustainable compounds for protecting newly planted seedlings, we evaluated the volatile metabolites produced by microbes isolated from H. abietis feces and frass. Female weevils deposit feces and chew bark at oviposition sites, presumably thus protecting eggs from feeding conspecifics. We hypothesize that microbes present in feces/frass are responsible for producing compounds that deter weevils. Here, we describe the isolation of a fungus from feces and frass of H. abietis and the biological activity of its volatile metabolites. The fungus was identified by morphological and molecular methods as Penicillium expansum Link ex. Thom. It was cultured on sterilized H. abietis frass medium in glass flasks, and volatiles were collected by SPME and analyzed by GC-MS. The major volatiles of the fungus were styrene and 3-methylanisole. The nutrient conditions for maximum production of styrene and 3-methylanisole were examined. Large quantities of styrene were produced when the fungus was cultured on grated pine bark with yeast extract. In a multi-choice arena test, styrene significantly reduced male and female pine weevils' attraction to cut pieces of Scots pine twigs, whereas 3-methylanisole only reduced male weevil attraction to pine twigs. These studies suggest that metabolites produced by microbes may be useful as compounds for controlling insects, and could serve as sustainable alternatives to synthetic insecticides.
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| 13. |
- Berasategui, Aileen, et al.
(författare)
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The Gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles
- 2016
-
Ingår i: Molecular Ecology. - : Wiley-Blackwell. - 0962-1083 .- 1365-294X. ; 25:16, s. 4014-4031
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Tidskriftsartikel (refereegranskat)abstract
- The pine weevil (Hylobius abietis, Coleoptera: Curculionidae) is an important pest of conifer seedlings in Europe. Despite its economic importance, little is known about the composition of its gut microbial community and the role it plays in mediating the weevil's ability to utilize conifers as a food source. Here, we characterized the gut bacterial communities of different populations of H. abietis across Europe and compared them to those of other beetles that occupy similar ecological niches. We demonstrate that the microbial community of H. abietis is similar at higher taxonomic levels (family and genus) across locations in Europe, with Wolbachia as the dominant microbe, followed by Enterobacteria and Firmicutes. Despite this similarity, we observed consistent differences between countries and locations, but not sexes. Our meta-analysis demonstrates that the gut bacterial community of the pine weevil is very similar to that of bark beetles that also exploit conifers as a food source. The Enterobacteriaceae symbionts of both host taxa are especially closely related phylogenetically. Conversely, the microbiota of H. abietis is distinct from that of closely related weevils feeding on non-conifer food sources, suggesting that the microbial community of the pine weevil is determined by the environment and may be relevant to host ecology. Furthermore, several H. abietis-associated members of the Enterobacteriaceae family are known to contain genes involved in terpenoid degradation. As such, we hypothesize that the gut microbial community is important for the utilization of conifer seedlings as a food source, either through the detoxification of plant secondary metabolites or supplementation of essential nutrients.
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| 14. |
- Björklund, Niklas, et al.
(författare)
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Exploiting jasmonate-induced responses for field protection of conifer seedlings against a major forest pest, Hylobius abietis
- 2014
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Ingår i: Forest Ecology and Management. - : Elsevier BV. - 0378-1127 .- 1872-7042. ; 313, s. 212-223
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Tidskriftsartikel (refereegranskat)abstract
- Herbivore damage commonly initiates an increased synthesis of chemical defensive compounds in attacked plants. Such induced defences are a vital part of plant defence systems, but when herbivore pressure is high, as frequently occurs in man-made ecosystems such as agricultural and forest plantations, plants may suffer considerable damage before adequate induced defences build up. To prepare the plants for such conditions their induced defence may be artificially triggered by the exogenous application of different phytohormones involved in damage signalling. This method is already employed in agriculture but within forestry systems it has so far been restricted to promising laboratory results. The pine weevil, Hylobius abietis, causes damage by feeding on the bark of young conifer plants and it is one of the main threats to successful regeneration in the Palaearctic region. Here we present results from a large scale field experiment where we triggered the induced defences of conifer seedlings using exogenous application of the chemical elicitor methyl jasmonate. To enhance the generality of the results different species were planted under extremely different environmental conditions; Maritime pine and Monterrey pine in Spain, and Scots pine and Norway spruce in Sweden. Weevil damage, chemical defences, and seedling growth were studied during the two growing periods following planting. In general, treated plants showed increased quantitative defences, and were less attacked, less wounded, less girdled and showed lower mortality rates than their untreated counterparts. Effects were mostly dose dependent, although some interactive effects with tree species were observed. The treatment initially caused a growth reduction but it was later compensated by the benefit, in terms of growth, of being less damaged. The measures that are currently taken to protect forest plantations against this harmful pest all around Europe have enormous economic costs and cause important environmental hazards. Elicitation of inducible defences in seedlings in the nursery appears to be an attractive alternative to these measures. To our knowledge, this is the first field study that explores the applicability of chemical elicitors of induced defences as a way to protect forest plantations against biotic threats. (C) 2013 Elsevier B.V. All rights reserved.
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| 15. |
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| 16. |
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| 17. |
- Björklund, Niklas, et al.
(författare)
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Snytbaggen : åtgärder för lyckade planteringar
- 2014
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Annan publikation (populärvet., debatt m.m.)abstract
- Barrträdsplantor som planterats efter avverkning blir ofta dödade av snytbaggens gnag. Men genom olika åtgärder kan skadorna minskas till en acceptabel nivå. Vilka metoder du bör välja beror bland annat på var i landet hygget ligger. Denna broschyr syftar till att förklara vilken effekt på plantskadorna olika åtgärder ger och hur de kan kombineras i olika delar av Sverige.
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| 18. |
- Björkman, Christer, et al.
(författare)
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A rare event – an isolated outbreak of the pine-tree lappet moth (Dendrolimus pini) in the Stockholm archipelago
- 2013
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Ingår i: Entomologisk tidskrift. - 0013-886X. ; 134, s. 1-9
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Tidskriftsartikel (refereegranskat)abstract
- The pine-tree lappet moth, Dendrolimus pini (L.), belonging to the family Lasiocampidae has a wide distribution in Eurasia. In Sweden it is normally found up to 61°N and normally occurs at low densities. In central Europe there have been several reports of outbreaks in historical time. In Sweden the last known outbreak before the one reported here was in 1938-40 in Värmland. In this paper we report a recent severe D. pini outbreak that was discovered in 2012 on the small island Furuskär in the Stockholm archipelago. The distribution of the damage on the island as well as a detailed inventory of larvae and pupae on individual trees is presented. Several photographs are presented to illustrate the damage. In the paper we formulate and try to answer several questions that arose as a natural consequence of this rare event: Why was this particular island hit? What are the causes behind the outbreak? What will happen to the trees and the vegetation on the island? Will the outbreak continue? Will the outbreak spread?
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| 19. |
- Björkman, Christer, et al.
(författare)
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Effects of new forest management on insect damage risk in a changing climate
- 2015
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Ingår i: Climate change and insect pests. - 9781780643786 ; 7, s. 248-266
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Bokkapitel (refereegranskat)abstract
- Recent findings suggest that damage by forest insect pests will increase as a consequence of climate warming. Here, we ask whether changes in forest management can alter and possibly mitigate the increased risk of damage and outbreaks. We focus mainly on conditions in northern Europe, particularly Sweden, but conclusions should be valid for northern temperate forests. Three types of insect pests are considered; the regeneration pest,Hylobius abietis(pine weevil), defoliators and the bark beetle,Ips typographus. We compare the expected effect of new management methods with the presently predominant method of even-aged stands, which are thinned two to three times before final harvest by clear-felling. Continuous cover forestry (CCF) is the method most different from the present practice. CCF would lead to a drastic decrease in pine weevils, and also less damage by defoliators, but this latter prediction is uncertain. For the bark beetle, the uncertainty is even greater. In mixed forests, all three insect pest types are expected to become less of a problem. Putative mechanisms involve more abundant and diverse natural enemy fauna, and a more scattered distribution of food resources. A shorter rotation period (including no thinning) is expected to increase the damage by pine weevils, as it will result in more abundant breeding material. For defoliators, it is difficult to foresee the effects. A shorter rotation period will decrease the risk of bark beetle outbreaks, as storm-fellings will be less frequent and young stands more common. The effects of exotic tree species and clonal forestry are complex and will depend on several factors. A general conclusion is that forest management may be used to mitigate the anticipated risk of insect pest damage as a consequence of climate warming, but more research is required to certify these indications
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| 20. |
- Cronkhite, J. T., et al.
(författare)
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Male and female germline specific expression of an EGFP reporter gene in a unique strain of transgenic rats
- 2005
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Ingår i: Dev Biol. ; 284:1, s. 171-83
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Tidskriftsartikel (refereegranskat)abstract
- A rat line was generated in which genomic integration of a ROSA-EGFP transgene resulted in exclusive expression of EGFP in the germ cells of both sexes. EGFP expression was uniform and robust in cleavage stage embryos beginning at the late 2-cell stage and continuing through blastocyst development where expression became restricted to cells of the inner cell mass. Subsequent analysis showed high EGFP expression exclusively in primordial, embryonic, and adult germ cells. This unique expression pattern makes this EGFP marked locus the first molecular marker of the germline lineage in both sexes in mammals. FISH was used to localize the transgene insertion to chromosome 11q11-q12, proximal to Grik1 and near Ncam2. Analysis of the region did not identify known germ cell-specific genes but did identify 19 ESTs or transcribed loci present in testes, ovary, or pre-implantation libraries from mice or rats. To assess the utility of the transgenic line for germ cell transplantation studies, non-selected, freshly isolated seminiferous tubule cells were transferred to the testis of recipient males. The donor cell population colonized the testis at a surprisingly high efficiency within 30 days following transfer. Since EGFP is a vital marker, the colonization process can be followed in vivo and the extent of colonization quantified. The unique germ cell specific expression of EGFP makes this line of transgenic rats an excellent novel tool to study germ cell origin, development, and differentiation, and to assess the plasticity of adult somatic stem cells to become male germ cells.
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| 21. |
- Dalin, Peter, et al.
(författare)
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Importance of temperature for the performance and biocontrol efficiency of the parasitoid Perilitus brevicollis (Hymenoptera: Braconidae) on Salix
- 2012
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Ingår i: BioControl. - : Springer Science and Business Media LLC. - 1386-6141 .- 1573-8248. ; 57, s. 611-618
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Tidskriftsartikel (refereegranskat)abstract
- With the prospect of warmer temperatures as a consequence of ongoing climate change, it is important to investigate how such increases will affect parasitoids and their top-down suppression of herbivory in agroecosystems. Here we studied how the performance and biocontrol efficiency of the willow "bodyguard" Perilitus brevicollis Haliday (Hymenoptera: Braconidae) were affected at different constant temperatures (10, 15, 20, 25A degrees C) when parasitizing a pest insect, the blue willow beetle (Phratora vulgatissima L., Coleoptera: Chrysomelidae). Parasitism did not reduce herbivory at all at 10A degrees C, indicating poor biocontrol efficiency at low temperatures. At higher temperatures, however, parasitism reduced herbivory substantially, implying that biocontrol may be promoted by a warmer climate. Parasitoid performance (survival and development rate) generally increased with increasing temperature up to 20A degrees C. The only exception was body size, which followed the temperature-size rule and decreased with increasing temperature. Our results indicate that a warmer climate may enhance the biocontrol of the blue willow beetle in environments that currently are cooler than the parasitoid's optimal temperature for development.
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| 22. |
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| 23. |
- Fedderwitz, Frauke, et al.
(författare)
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Diel behaviour and time budget of the adult pine weevil Hylobius abietis
- 2014
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Ingår i: Physiological Entomology. - : Wiley. - 0307-6962 .- 1365-3032. ; 39, s. 103-110
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Tidskriftsartikel (refereegranskat)abstract
- The pine weevil [Hylobius abietis (L.); Coleoptera: Curculionidae] has a high economic impact on forest regeneration in Europe. The general biology of the pine weevil has received considerable attention, although there is insufficient knowledge about its diel behaviour and time budget. Therefore, in the present study, the feeding and locomotion behaviour of individual adult weevils on Norway spruce seedlings [Picea abies (L.) Karst.] is observed for 24-h periods in the laboratory. Both girdled and nongirdled seedlings are used to assess how the behaviour of weevils is influenced by the physiological response of plants to the girdling. The locomotion pattern shows a distinct maximum during the beginning of the dark phase, whereas most feeding occurs during the second half of the dark phase and the firsthours of the subsequent light phase. The girdling treatment increases the time that weevils spend on the seedlings during the first part of an observation session, although it has no effect on their feeding pattern. The time budgets of weevils on girdled and nongirdled seedlings are similar. On average, weevils spend 34% of their time in locomotion and 6% on feeding. Females spend more time feeding than males (7.1% versus 4.2%), possibly because they have higher food requirements (e.g. for egg production). Females also spend more time in total on the seedlings than males (26.3% versus 7.0%). The present study reveals, in high temporal resolution, the diel feeding and locomotion behaviour and time budget of male and female pine weevils.
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| 24. |
- Fedderwitz, Frauke, et al.
(författare)
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Does the pine weevil (Hylobius abietis) prefer conifer seedlings over other main food sources?
- 2018
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Ingår i: Silva Fennica. - : Finnish Society of Forest Science. - 0037-5330 .- 2242-4075. ; 52
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Tidskriftsartikel (refereegranskat)abstract
- Adult pine weevils (Hylobius abietis (L.)) feed on the tender bark of branches and roots of mature conifer trees and on the stem bark of conifer seedlings. Their feeding on mature trees does not cause any economic damage, but their feeding on planted seedlings is so devastating that the pine weevil is considered one of the most important forest pest insects in Europe. We asked whether the pine weevil prefers seedlings over other regularly utilized food sources. This question is of particular interest because new approaches to seedling protection are based on decreasing any preference for seedlings by using less palatable plants or by enhancing their defence (by genetic selection or by methyl jasmonate treatment). In a laboratory choice experiment we tested pine weevil feeding preferences for seedlings compared with branches and roots from mature trees (separately for Norway spruce and Scots pine). Pine weevils preferred roots, but not branches, of Norway spruce over seedlings of the same species. With Scots pine there were no clear preferences, but the weevils showed a tendency to prefer roots over seedlings. These results provide support for seedling protection approaches that attempt to redirect pine feeding from planted seedlings to other food sources.
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| 25. |
- Fedderwitz, Frauke, et al.
(författare)
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Effects of jasmonate-induced resistance in conifer plants on the feeding behaviour of a bark-chewing insect, Hylobius abietis
- 2016
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Ingår i: Journal of Pest Science. - : Springer Science and Business Media LLC. - 1612-4758 .- 1612-4766. ; 89, s. 97-105
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Tidskriftsartikel (refereegranskat)abstract
- Conifer defences can be induced by exogenous chemical elicitors, thereby reducing damage caused by bark-feeding insects. However, the insect behavioural mechanisms involved are poorly understood. Thus, effects of artificially induced plant defences on feeding behaviour of the pine weevil (Hylobius abietis), a serious forest pest, were examined to explore mechanisms involved in decision- making processes connected to feeding. To induce plant defences, we applied methyl jasmonate (MeJA), a naturally occurring plant hormone, to young Norway spruce (Picea abies) plants. The weevils’ feeding behaviour on plants with and without MeJA treatment was studied in both a no-choice and a choice laboratory experiment. MeJA treatment did not affect the initiation of feeding, but it affected the weevils’ subsequent feeding patterns. In the no-choice experiment, the only observed effect of its treatment was that it reduced the size of the initial feeding scars. In the choice experiment, it reduced both the numbers and sizes of the feeding scars and hence the total debarked area. Thus, the MeJA-induced resistance did not deter the pine weevils from attacking the spruce plants, but reduced the amounts they consumed at one place, which would reduce risks of girdling and plant death. This may be the behavioural mechanism behind the previously recorded increases in survival rates of MeJAtreated plants in the field.
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| 26. |
- Fedderwitz, Frauke, et al.
(författare)
-
Pine weevil (Hylobius abietis) feeding pattern on conifer seedlings.
- 2012
-
Konferensbidrag (refereegranskat)abstract
- The pine weevil (Hylobius abietis) is one of the most important forest pests in Europe, yet there is very littleknown about its detailed feeding behaviour. We study the temporal feeding pattern of individual pine weevils ofboth sexes for 24 hours with two treatments, intact and girdled seedlings. Properties of a meal, such as feedingduration, size and ingestion rate are of particular interest. The shortest interval considered to separate onefeeding bout from another, the meal criterion, has never been published and it is only available for a few otherinsect species. Video recordings are analysed for feeding behaviour (e.g. duration of feeding activity, intervallength between feeding activities, movements between and within feeding scars). We measured general activitypatterns as there is insufficient knowledge on the daily behavioural patterns. We thereby got an in-depth view ofthe pine weevil feeding activity that would otherwise be difficult to assess.
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| 27. |
- Fedderwitz, Frauke, et al.
(författare)
-
The structure of feeding behavior in a phytophagous insect (Hylobius abietis)
- 2015
-
Ingår i: Entomologia Experimentalis et Applicata. - 0013-8703 .- 1570-7458. ; 155, s. 229-239
-
Tidskriftsartikel (refereegranskat)abstract
- Analysis of the feeding behavior of animals using such a high temporal resolution that meals can be defined may improve our understanding of the mechanisms regulating feeding. Meals can be distinguished in an ethologically meaningful manner by using the ‘meal criterion’, the shortest non-feeding interval between feeding bouts recognized as meals. However, such a criterion has only been determined for a few insect species. Applying a recent method developed for assessing meal criteria for vertebrates, we determined the meal criterion for Hylobius abietis (L.) (Coleoptera: Curculionidae) based on data from video recordings of single individuals feeding on seedlings of Norway spruce, Picea abies (L.) Karst. (Pinaceae). The pine weevil is an economically important pest insect because it feeds on the stem bark of planted conifer seedlings. Weevils had 4-5 meals per day. Each meal lasted about 24 min during which about 13 mm2 of bark per meal were removed. Females had longer total meal durations and longer non-feeding intervals within meals than males. Girdling seedlings did not affect the weevils’ feeding properties. The size of meals was significantly correlated to the duration of non-feeding intervals before and after them. This study is one of few describing the feeding behavior of an insect at a temporal resolution that allows individual meals to be distinguished. With more meal-related data from insects available, differences in meal properties may be interpreted based on phylogeny, ecology, and physiology. Our results may also assist in the setup and interpretation of studies of plant-insect interactions, and facilitate the evaluation and development of methods to protect plants against herbivores.
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| 28. |
- Forshage, Mattias, et al.
(författare)
-
Eucoilinae of North America: a revised catalog of genera and described species
- 2013
-
Ingår i: Proceedings of the Entomological Society of Washington. - : Entomological Society of Washington. - 0013-8797. ; 115:3, s. 225-255
-
Tidskriftsartikel (refereegranskat)abstract
- We present an updated catalog of North American Eucoilinae, bearing little resemblance to previous regional catalogs, which have been lagging behind in the recent systematic work in the group. The current catalog comprises 34 genera, arranged in six tribes. Of these genera, 9 are represented wholly by unidentified or undescribed species in the region, while the other 25 include 108 species recorded from the region. In comparison with previous catalogs, 24 genera and 41 species are added, and 34 species-level names are recombined, while 7 genera, five subgenera and 11 species are removed from the list of North American taxa. A modern, phylogenetically stable and type-based classification is implemented, as well as a tribal classification. In terms of nomenclatural acts, 25 new combinations are made (one is a reinstatement of an old combination); four new genus-levels synonymies are made (Tetramerocera Ashmead junior synonym of Ganaspis Fo ̈rster, Bewelda Quinlan and Aporeucoela Kieffer junior synonyms of Hexacola Fo ̈rster, Pentaplastidia Weld junior synonym of Trybliographa Fo ̈rster); eight new species-level synonymies are made; two species names are removed from synonymy; one new replacement name given (Hexacola pennsylvanicus for Hexacola subaperta Kieffer 1907 nec (Kieffer 1901a)).
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| 29. |
- Forshage, Mattias, 1968-, et al.
(författare)
-
Identification key to European genera of Eucoilinae (Hymenoptera, Cynipoidea, Figitidae)
- 2008
-
Ingår i: Insect Systematics & Evolution. - 1399-560X .- 1876-312X. ; 39:3, s. 341-359
-
Tidskriftsartikel (refereegranskat)abstract
- A key is presented to the European genera of Eucoilinae, for the first time after these genera have been circumscribed based on type studies and phylogenetic concerns. The key is illustrated with drawings based on SEM pictures. A generic catalogue with full synonymy of the European eucoiline genera is presented. The following new synonymies on the generic level are proposed: Trichoplasta Benoit, 1956 (Armigerina Belizin, 1968 syn.n.), Eucoila Westwood,1833 (Lenobria Belizin, 1968 syn.n.), Kleidotoma Westwood, 1833 (Nesokleidotoma Beardsley, 1990 syn.n., Octameris Belizin, 1973 syn.n., Polbourdouxia Dessart, 1977 syn.n., Pentakleidota Weld, 1951 syn.n.). One species level synonymy is proposed: Eucoila floralis Dahlbom, 1846 (Lenobria bidentata Belizin, 1968 syn.n.) A tribal classification of the subfamily is utilised, recognizing the following tribes: Diglyphosemini Belizin, 1961 stat.n.,Kleidotomini Hellén, 1960 stat.n. (Cothonaspini Belizin, 1961 syn.n.), Ganaspini Belizin,1961 stat.n. (Dieucoilini Belizin, 1961 syn.n., Glauraspidiini Belizin, 1961 syn.n.),Trichoplastini Kovalev, 1989 and Eucoilini Thomson, 1862.
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| 30. |
- Forshage, Mattias, 1968-, et al.
(författare)
-
Quasimodoana, a new Holarctic genus of eucoiline wasps (Hymenoptera, Cynipoidea, Figitidae), with a phylogenetic analysis of related genera
- 2008
-
Ingår i: Systematic Entomology. - : Wiley. - 0307-6970 .- 1365-3113. ; 33:2, s. 301-318
-
Tidskriftsartikel (refereegranskat)abstract
- A new Holarctic genus, Quasimodoana gen.n. is described for the Palearctic eucoiline wasp Eucoila decipiens Förster, 1869, as Quasimodoana decipiens comb.n. A lectotype is designated for this species. A new North American species is described here as Quasimodoana gibba sp.n. As the new genus has similarities with several eucoiline genera, belonging to two related but distinct lineages, a phylogenetic analysis was carried out, based on 83 morphological characters. The two lineages included in the analysis are referred to here under the tribal names of Eucoilini and Trichoplastini stat.nov. We discuss reasons for adopting available tribal names (rather than 'informal genus groups'), and Trichoplastini is removed from synonymy with Eucoilini. The phylogenetic analysis places Quasimodoana unambiguously within Eucoilini, as a sistergroup to the Trybliographa complex. However, there is only weak support for the monophyly of Eucoilini and for the hypothesized sistergroup relationship between Eucoilini and Trichoplastini.
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| 31. |
- Forshage, Mattias, 1968-
(författare)
-
Systematics of Eucoilini : Exploring the diversity of a poorly known group of Cynipoid parasitic wasps
- 2009
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Many animal species are still undiscovered, even in Sweden. This thesis deals with the wasps of the subfamily Eucoilinae (of Figitidae, Cynipoidea), a poorly known group of small parasitoids of Dipteran flies. In this group, old classifications are chaotic, knowledge of old taxa is scant, phylogenetic work has recently started, and many new taxa remain to be described. In this thesis, an identification key is given to the European genera of Eucolinae, and a tribal classification of the subfamily is reintroduced, based on recent phylogenetic results. Most of the thesis focuses on the tribe Eucoilini. That tribe is analyzed phylogenetically in two analyses, one based on a large morphological dataset, the other on two molecular markers, ribosomal 28S and mitochondrial Cytochrome B. The monophyletic core group of Eucoilini is the Eucoila/Trybliographa complex. It is treated in a big taxonomic revision, based on studies of several hundred possibly relevant type specimens and several thousands of other specimens, and on the phylogenetic analyses. More than 500 names of possible members of this group are investigated, and many new combinations are proposed, of species found not to belong within the group, as well as those that did, including some which are transferred to other genera in accordance with the new phylogenetic results. The new genus Quasimodoana is erected, and 20 new species described (8 from Northern Europe, 12 from elsewhere). More than 180 new combinations, more than 50 new species-level synonyms and ca 10 genus-level synonyms, are proposed. Within this complex, the basal split is found to have been between a Paleotropic, specis-poor lineage (Bothrochacis), and a species-rich and largely Holarctic lineage, dominated by Trybliographa but also including the small genera Eucoila and Linoeucoila. The three are not satisfactorily resolved in the analysis, but still maintained as separate genera for pragmatic reasons. It is hypothesized that this group originated in Asia during the Eocene or Oligocene, attacking calyptrate flies in herbivore dung. Then it separated in a tropical branch retaining the ancestral life history mode, and a Holarctic branch, where some lineages specialised in Anthomyiidae and colonised the microhabitats of fungi and plants, speciating extensively in the boreal forests.
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| 32. |
- Forshage, Mattias, et al.
(författare)
-
The identity of figitid parasitoids (Hymenoptera: Cynipoidea: Figitidae) of anthomyiid flies in conifer cones
- 2018
-
Ingår i: European Journal of Entomology. - : Biology Centre, AS CR. - 1210-5759 .- 1802-8829. ; 115, s. 104-111
-
Tidskriftsartikel (refereegranskat)abstract
- Larvae of Strobilomyia flies (Diptera: Anthomyiidae) are serious pests in conifer-seed orchards because they feed on the seed inside the cones. Figitid parasitoids (Hymenoptera: Cynipoidea) of Strobilomyia larvae in conifer cones are commonly reported but under various generic names. It is argued here that, across the entire Holarctic region, these figitids belong to Amphithectus and perhaps also to Sarothrus (Figitinae), but not to Melanips (Aspicerinae), contrary to some reports. We conclude that the identity of the commonly found figitid associated with conifer cones (Larix and Picea) in Europe and Asia is Amphithectus austriacus (Tavares, 1928) comb. n. This is most likely considering the original description and the host association, although the type specimen of Seitneria austriaca Tavares, 1928 is lost. This species name takes priority over the recently described Amphithectus coriaceus Paretas-Martinez & Pujade-Villar, 2013. Seitneria Tavares, 1928 becomes a new junior synonym of Amphithectus Hartig, 1840, and Amphithectus coriaceus Paretas-Martinez & Pujade-Villar, 2013 becomes a new synonym of Amphithectus austriacus (Tavares, 1928) comb. n.
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| 33. |
- Hellqvist, Claes, et al.
(författare)
-
Ökade plantskador av bastborrar
- 2014
-
Ingår i: Skogseko. - 0346-0495. ; 29, s. 40-41
-
Tidskriftsartikel (populärvet., debatt m.m.)
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| 34. |
- Kanuch, Peter, et al.
(författare)
-
Parapatric Genetic Lineages Persist in a Multiply Introduced Non-native Bush-Cricket
- 2022
-
Ingår i: Frontiers in Ecology and Evolution. - : Frontiers Media SA. - 2296-701X. ; 10
-
Tidskriftsartikel (refereegranskat)abstract
- To understand colonization success of an invasive species we need to know the origin of the founders, where and when they were introduced, and how they spread from the introduction site(s) through the landscape. Admixture of different genetic lineages from multiple introductions is generally hypothesized to be beneficial to invasive species thanks to adaptive variation and heterozygosity-fitness correlations. In this study, population genetic and landscape data was gathered for Roesel's bush-cricket, Roeseliana roeselii a small bush-cricket common in central and eastern Europe that currently is expanding its range in northern Europe. We examined how colonization history and landscape structure affect the spread of the species and its population genetic structure, as a consequence of multiple introductions. Using comprehensive information of the species ecology and dispersal, together with genetic structure inferred from samples from 29 locations in central Sweden (we employed data published by Preuss et al., 2015), we found that two parapatric founding lineages have coexisted with very little gene flow during a long time span. An isolation-by-distance pattern and a decrease of genetic diversity toward marginal areas were more pronounced in the lineage situated in forest dominated landscapes. Our findings are in strong contrast to the hypothesis that different genetic lineages will admix when introduced to the same area. The presence of the separate lineages decades after introduction and without physical barriers for gene flow shows that some mechanism prevents them from admixture. One possibility is that the lineages with different genetic setups have adapted independently to local conditions and their admixture resulted in loss of locally adapted genotypes and hybrid offspring, less viable than the respective ancestral genotypes. However, an alternative post-mating reproductive barrier and hybrid breakdown phenomenon should also be considered. Our data indicate that besides landscape characteristics, human transportation of agricultural goods may play an important role for the overall spatial genetic pattern of the species in the study area by aiding the spread of the species.
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| 35. |
- Krantz, Marcus, 1975, et al.
(författare)
-
Robustness and fragility in the high osmolarity glycerol (HOG) pathway in S. cerevisiae
- 2009
-
Ingår i: 10th International Conference on Systems Biology (ICSB2009) proceedings, 10th International Conference on Systems Biology (ICSB2009), Aug 30 - Sep 4, Stanford, California, USA.
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Cellular signalling networks integrate environmental stimuli with information on cellular status. These networks must be robust against stochastic fluctuations in external stimuli as well as in the amounts of signalling components. Here [1], we challenge the yeast HOG signal transduction pathway with systematic perturbations in components’ expression levels implemented by a “genetic tug-of-war” methodology under various external conditions in search of nodes of fragilities. We observe a substantially higher frequency of fragile nodes in this signal transduction pathway than has been observed for other cellular processes. These fragilities disperse without any clear pattern over biochemical functions or location in pathway topology, with the most sensitive nodes being the proteins PBS2 and SSK1. They are also largely independent of pathway activation by external stimuli. However, the strongest toxicities are caused by pathway hyperactivation. We studied the influence of seven regulatory motifs around these HOG pathway components in silico through ODE models. Based on the SLN1 and the MAPK modules of a mathematical model of osmoregulation in budding yeast by Klipp et al. [2] we included new motifs and fitted the affected parameters to time courses of dually phosphorylated Hog1p generated by the original model under stress and stress-free conditions. The regulations taken into account by our analysis include Pbs2p scaffolding, Ssk1p and Pbs2p autoactivation, and the formation of a stable dimer between Ssk2p and Ssk1p. A subsequent sensitivity analysis identified Pbs2's role as a scaffold protein and Ssk1p-Ssk2p dimerization as the important contributors to the observed robustness pattern in silico. Thus, in vivo robustness data can be used to discriminate and improve mathematical models.
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| 36. |
- Krantz, Marcus, 1975, et al.
(författare)
-
Robustness and fragility in the yeast high osmolarity glycerol (HOG) signal-transduction pathway.
- 2009
-
Ingår i: Molecular systems biology. - : EMBO. - 1744-4292. ; 5
-
Tidskriftsartikel (refereegranskat)abstract
- Cellular signalling networks integrate environmental stimuli with the information on cellular status. These networks must be robust against stochastic fluctuations in stimuli as well as in the amounts of signalling components. Here, we challenge the yeast HOG signal-transduction pathway with systematic perturbations in components' expression levels under various external conditions in search for nodes of fragility. We observe a substantially higher frequency of fragile nodes in this signal-transduction pathway than that has been observed for other cellular processes. These fragilities disperse without any clear pattern over biochemical functions or location in pathway topology and they are largely independent of pathway activation by external stimuli. However, the strongest toxicities are caused by pathway hyperactivation. In silico analysis highlights the impact of model structure on in silico robustness, and suggests complex formation and scaffolding as important contributors to the observed fragility patterns. Thus, in vivo robustness data can be used to discriminate and improve mathematical models.
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| 37. |
- Krantz, Marcus, 1975, et al.
(författare)
-
Robustness and fragility in the yeast High Osmolarity signal transduction pathway
- 2008
-
Ingår i: 2008 Yeast Genetics and Molecular Biology Meeting Program and Abstract Book, 2008 Yeast Genetics and Molecular Biology Meeting, July 22-27, 2008.
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The cellular signalling networks that integrate various environmental stimuli with information on cellular status must be robust to stimuli fluctuations as well as to stochastic differences in the amounts of signalling components. Here, we challenge the Hog signal transduction pathway with systematic disturbances in components’ expression levels implemented by a “genetic tug-of-war”, or gToW, methodology. The disturbances were performed under various external perturbations, including pathway activation by osmotic shock. Ideally, the obtained sensitivity profiles will allow us to impose parameter constraints. However, a more important aspect is the qualitative improvement of model structures, when local fragilities cannot be explained by the model structure. The resulting phenotypes in this particular study reflect a wide range of sensitivities, and disperse without any clear pattern over biochemical functions and pathway modules alike, with the most sensitive nodes being PBS2 and SSK1. Surprisingly, the “neighbouring” nodes HOG1 and SSK2 were affected to a much lesser extent, questioning our current understanding.
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| 38. |
- Krantz, Marcus, 1975, et al.
(författare)
-
Robustness and fragility in the yeast High Osmolarity signal transduction pathway
- 2008
-
Ingår i: 9th International Conference on Systems Biology (ICSB2008) proceedings, 9th International Conference on Systems Biology (ICSB2008), August 22-28, Gothenburg.
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Objective: The cellular signalling networks that integrate various environmental stimuli with information on cellular status must be robust to stimuli fluctuations as well as to stochastic differences in the amounts of signalling components. Here, we challenge the high osmolarity glycerol response (HOG) signal transduction pathway in the yeast Saccharomyces cerevisiae with systematic disturbances in components’ expression levels implemented by a “genetic tug-of-war”, or gTOW, methodology. Results: The disturbances were performed under various external perturbations, including pathway activation by osmotic shock. The resulting phenotypes in this particular study reflect a wide range of sensitivities, and disperse without any clear pattern over biochemical functions and pathway modules alike, with the most sensitive nodes being PBS2 and SSK1. Conclusions: Ideally, the obtained sensitivity profiles will allow us to impose parameter constraints. However, a more important aspect is the qualitative improvement of model structures, when local fragilities cannot be explained by the model structure. Surprisingly, the “neighboring” nodes HOG1 and SSK2 were affected to a much lesser extent, questioning our current understanding.
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| 39. |
- Kännaste, Astrid, et al.
(författare)
-
Volatiles from a Mite-Infested Spruce Clone and Their Effects on Pine Weevil Behavior
- 2009
-
Ingår i: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 35:10, s. 1262-1271
-
Tidskriftsartikel (refereegranskat)abstract
- Induced responses by Norway spruce (Picea abies) seedlings to feeding damage by two mite species were studied by analyzing the volatiles emitted during infestation. Four specimens of a Norway spruce (Picea abies L.) clone were infested with mites of Nalepella sp., another four with Oligonychus ununguis, and four were kept mite-free as controls. After a year of infestation, spruce volatiles were collected, analyzed, and identified using SPME-GC-MS. In addition, enantiomers of chiral limonene and linalool were separated by two-dimensional GC. Methyl salicylate (MeSA), (-)-linalool, (E)-beta-farnesene, and (E,E)-alpha-farnesene were the main volatiles induced by both species of mites, albeit in different proportions. The ability of the main compounds emitted by the mite-infested spruces to attract or repel the pine weevil, Hylobius abietis (L.), was tested. (E)-beta-farnesene was found to be attractive in the absence of spruce odor, whereas methyl salicylate had a deterrent effect in combination with attractive spruce odor. The other tested compounds had no significant effects on the behavior of the weevils.
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| 40. |
- Lindelöw, Åke, et al.
(författare)
-
Svart granbastborre
- 2017
-
Ingår i: Skogsskötselserien. ; , s. 65-68
-
Annan publikation (populärvet., debatt m.m.)abstract
- Svart granbastborre har en liknande biologi som snytbaggen och arterna förekommer ofta tillsammans. Planterade granplantor skadas genom de fullbildade bastborrarnas gnag i barken på rötter och nedersta delen av stammen. Märkligt nog har i Sverige inga mer omfattande plantskador förorsakade av den lika allmänna svarta tallbastborren noterats i tallplanteringar.
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| 41. |
- Liu, Zhiwei, et al.
(författare)
-
The cynipoid genus Paramblynotus : Revision, phylogeny, and historical biogeography (Hymenoptera : Liopteridae)
- 2007
-
Ingår i: Bulletin of the American Museum of Natural History. - 0003-0090 .- 1937-3546. ; :304, s. 3-151
-
Tidskriftsartikel (refereegranskat)abstract
- The genus Paramblynotus is the most species-rich genus of the so-called macrocynipoids, the large cynipoid parasitoids of wood-boring and cone-boring insect larvae. The species range in size from some of the largest to the smallest macrocynipoids, comparable in size to microcynipoids. Paramblynotus members occur on all continents except Europe and Australia, with most species being tropical or subtropical. The biology is poorly known but a few observations indicate that the species are parasitoids of beetle larvae. In this monographic revision of the genus, we present a species-level cladistic analysis based on qualitative and quantitative features of the external morphology. For analysis of quantitative features, we present for the first time a novel coding method, the method of Finite Mixture Coding (FMC) based on k-means clustering, or FMCK. The new method is similar to the FMC method proposed by previous authors in that they both generate codes (character states) for phylogenetic analysis as the direct output of a statistical procedure, thus avoiding the subdivision of quantitative data into discrete states on the basis of arbitrary criteria as with other coding methods. Through incorporating finite mixture analysis and likelihood estimation as used in FMC and k-mean cluster analysis for a priori statistical modeling of component distributions, FMCK is advantageous over FMC in that it can be implemented using readily available statistic programs with k-mean cluster analysis, such as STATISTICA, MINITAB or SYSTAT, available on both PC and Macintosh platforms. We were able to identify 8 quantitative characters among 23 as useful for cladistic analysis by using the new coding method. In total, our character matrix has 132 coded characters. The phylogenetic analysis indicates that species of the previously recognized genus Decelleu form a monophyletic group deeply nested within Paramblynotus. Decellea is therefore synonymized with Paramblynotus, which is separated into seven monophyletic species groups: the virginianus, scaber, ynngambicolars, nigricornis, apeosus, ruficollis, and punclulalus groups. Based on the phylogeny, we reconstruct the historical biogeography of the liopterid subfamily Mayrellinae, consisting of the genera Paramblynotus and Kiefferiellu, using dispersal-vicariance analysis in combination with palaeoenvironmental data. The results suggest that the subfamily originated in the Northern Hemisphere and then expanded its distribution early by way of the Bering area. The divergence between Puramblynotus and Kiefferiellu was apparently associated with the formation of the Rocky Mountains about 50 million years ago. An early Paramblynotus lineage dispersed to Africa from the eastern Palearctic by way of Arabia, and it subsequently diversified along with montane forests in Africa. The relatively high diversity of Paramblynotus in Southeast Asia is considered to be partly caused by the frequent sea level changes since late Oligocene (29 Ma), which drastically changed the land configuration of this area. We end this paper with a taxonomic revision of the genus Paramblynotus, with a total of 92 species treated, including 72 described as new and 20 previously known, of which 18 are redescribed. Keys to the species groups as defined in this paper and to all known species of each species group are provided. For all species, the available information on their biology and distribution are summarized.
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| 42. |
- Lundborg, Lina, 1986-, et al.
(författare)
-
Conifer chemical defenses influence meal properties of the pine weevil Hylobius abietis
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Chemical defenses of conifers can be experimentally activated to respond more efficiently to feeding damage by insects. One chemical elicitor that triggers plant defenses, and thereby protects conifers, is methyl jasmonate (MeJA). However, there is little known about the associations between MeJA-induced conifer defenses, and the meal properties of phytophagous insects. To address this knowledge gap, we have analyzed relations between volatile contents of Scots pine (Pinus sylvestris L.) tissues, and meal properties of the pine weevil (Hylobius abietis (L.)). Phloem and needles (both weevil target tissues) from MeJA-treated and control seedlings were extracted by n-hexane and analyzed by two-dimensional gas chromatography-mass spectrometry (2D GC-MS). Feeding of pine weevils on seedlings from the same batches were video-recorded to determine meal properties. Multivariate statistical analyses showed that phloem and needle contents of MeJA-treated seedlings had different volatile compositions from the control seedlings. Levels of (+)-α-pinene were high, and levels of the antifeedant 2-phenylethanol were low, in phloem of control seedlings with feeding damage. Accordingly, pine weevils fed more slowly and had shorter meals on MeJA-treated seedlings. In addition, the chemical compositions of phloem and needles – and accordingly weevil meal durations on them – were more similar in MeJA-treated seedlings than in controls. The results illustrate that detailed knowledge of insect responses can help efforts to identify and elucidate specific roles of resistance agents in complex chemical profiles.
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| 43. |
- Lundborg, Lina, 1986-, et al.
(författare)
-
Induced defenses change the chemical composition of pine seedlings and influence meal properties of the pine weevil Hylobius abietis
- 2016
-
Ingår i: Phytochemistry. - : Elsevier. - 0031-9422 .- 1873-3700. ; 130, s. 99-105
-
Tidskriftsartikel (refereegranskat)abstract
- The defense of conifers against phytophagous insects relies to a large extent on induced chemical defenses. However, it is not clear how induced changes in chemical composition influence the meal properties of phytophagous insects (and thus damage rates). The defense can be induced experimentally with methyl jasmonate (MeJA), which is a substance that is produced naturally when a plant is attacked. Here we used MeJA to investigate how the volatile contents of Scots pine (Pinus sylvestris L.) tissues influence the meal properties of the pine weevil (Hylobius abietis (L.)). Phloem and needles (both weevil target tissues) from MeJA-treated and control seedlings were extracted by n-hexane and analyzed by two-dimensional gas chromatography-mass spectrometry (2D GC-MS). The feeding of pine weevils on MeJA-treated and control seedlings were video-recorded to determine meal properties. Multivariate statistical analyses showed that phloem and needle contents of MeJA-treated seedlings had different volatile compositions compared to control seedlings. Levels of the pine weevil attractant (+)-α-pinene were particularly high in phloem of control seedlings with feeding damage. The antifeedant substance 2-phenylethanol occurred at higher levels in the phloem of MeJA-treated than in control seedlings. Accordingly, pine weevils fed slower and had shorter meals on MeJA-seedlings. The chemical compositions of phloem and needle tissues were clearly different in control seedlings but not in the MeJA-treated seedlings. Consequently, meal durations of mixed meals, i.e. both needles and phloem, were longer than phloem meals on control seedlings, while meal durations on MeJA seedlings did not differ between these meal contents. The meal duration influences the risk of girdling and plant death. Thus our results suggest a mechanism by which MeJA treatment may protect conifer seedlings against pine weevils.
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| 44. |
- Lundborg, Lina, 1986-, et al.
(författare)
-
Methyl jasmonate-induced monoterpenes in Scots pine and Norway spruce tissues affect pine weevil orientation
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- In large parts of Europe insecticide-free measures for protecting conifer plants are desired to suppress damage by the pine weevil Hylobius abietis (L.). Treatment with methyl jasmonate (MeJA), a chemical elicitor already used in crop production, may enhance expression of chemical defenses in seedlings in conifer regenerations. However, in a previous experiment MeJA treatment resulted in substantially better field protection for Scots pine (Pinus sylvestris L.) than for Norway spruce (Picea abies (L.) Karst.). Hypothesizing that the variations may be at least partly due to volatiles released by MeJA-treated seedlings and their effects on pine weevil orientation, we examined tissue extracts of seedlings (from the same batches as previously used) by two-dimensional GC-MS. We found that the MeJA treatment increased contents of the monoterpene (-)-β-pinene in phloem (the weevil’s main target tissue) of both tree species, however, the (-)-β-pinene/(-)-α-pinene ratio increased more in the phloem of P. sylvestris. We also tested the attractiveness of individual monoterpenes found in conifer tissues (needles and phloem) for pine weevils using an arena with traps baited with single-substance dispensers and pine twigs. Trap catches were reduced when the pine material was combined with a dispenser releasing (-)-β-pinene, (+)-3-carene, (-)-bornyl acetate or 1,8-cineole. However, (-)-α-pinene did not have this effect. Thus, the greater field protection of MeJA-treated P. sylvestris seedlings may be due to the selective induction of increases in contents of the deterrent (-)-β-pinene, in contrast to strong increases in both non-deterrent (-)-α-pinene and the deterrent (-)-β-pinene in P. abies seedlings.
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| 45. |
- Lundborg, Lina, et al.
(författare)
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Methyl Jasmonate-Induced Monoterpenes in Scots Pine and Norway Spruce Tissues Affect Pine Weevil Orientation
- 2016
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Ingår i: Journal of Chemical Ecology. - : Springer-Verlag New York. - 0098-0331 .- 1573-1561. ; 42:12, s. 1237-1246
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Tidskriftsartikel (refereegranskat)abstract
- In large parts of Europe, insecticide-free measures for protecting conifer plants are desired to suppress damage by the pine weevil Hylobius abietis (L.). Treatment with methyl jasmonate (MeJA), a chemical elicitor already used in crop production, may enhance expression of chemical defenses in seedlings in conifer regenerations. However, in a previous experiment, MeJA treatment resulted in substantially better field protection for Scots pine (Pinus sylvestris L.) than for Norway spruce (Picea abies (L.) Karst.). Hypothesizing that the variations may be at least due partly to volatiles released by MeJA-treated seedlings and their effects on pine weevil orientation, we examined tissue extracts of seedlings (from the same batches as previously used) by two-dimensional GC-MS. We found that the MeJA treatment increased contents of the monoterpene (-)-beta-pinene in phloem (the weevil's main target tissue) of both tree species, however, the (-)-beta-pinene/(-)-alpha-pinene ratio increased more in the phloem of P. sylvestris. We also tested the attractiveness of individual monoterpenes found in conifer tissues (needles and phloem) for pine weevils using an arena with traps baited with single-substance dispensers and pine twigs. Trap catches were reduced when the pine material was combined with a dispenser releasing (-)-beta-pinene, (+)-3-carene, (-)-bornyl acetate or 1,8-cineole. However, (-)-alpha-pinene did not have this effect. Thus, the greater field protection of MeJA-treated P. sylvestris seedlings may be due to the selective induction of increases in contents of the deterrent (-)-beta-pinene, in contrast to strong increases in both non-deterrent (-)-alpha-pinene and the deterrent (-)-beta-pinene in P. abies seedlings.
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| 46. |
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| 47. |
- Manak, Vita, et al.
(författare)
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Behavioural responses of pine weevils to non-consumptive interactions with red wood ants
- 2016
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Ingår i: Journal of Zoology. - : Wiley. - 0952-8369 .- 1469-7998. ; 299, s. 10-16
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Tidskriftsartikel (refereegranskat)abstract
- Non-consumptive effects are increasingly recognized as important in predator-herbivore interactions, with both fundamental and practical implications. Notably, feeding damage to conifer seedlings by pine weevils, Hylobius abietis (L.), which are serious pests in forest regeneration areas, can be decreased by interactions with ants (Formicidae). To probe the behavioural mechanisms underlying such effects, which have rarely been described in predator-herbivore systems, we investigated how the ants influence the weevils' behaviour around planted conifer seedlings in the field. Half of the seedlings were infested with aphids and attended by red wood ants Formica polyctena Forster, whereas the others were used as controls. We asked whether the ants' behaviour towards the weevils differs between these treatments, and whether the weevils' movement behaviour (locomotion, stationary activity and digging) is affected by the treatments and physical interactions with ants. The ants attacked pine weevils more frequently and for longer periods around ant-attended seedlings than around controls. Weevils' locomotion behaviour also increased markedly when attacked by ants. This may increase the likelihood of pine weevils moving away from, and causing less damage to, ant-attended seedlings. This study demonstrates how non-consumptive effects can be explained in terms of a herbivore's responses to a predator's aggressive behaviours.
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| 48. |
- Manak, Vita, et al.
(författare)
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Testing associational resistance against pine weevils mediated by Lasius ants attending conifer seedlings
- 2017
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Ingår i: Journal of Applied Entomology. - : Wiley. - 0931-2048 .- 1439-0418. ; 141, s. 411-416
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Tidskriftsartikel (refereegranskat)abstract
- Natural enemies attracted to plants may provide those plants with protection against herbivores but may also protect neighbouring plants, that is through associational resistance. Ant attendance may be an important mechanism for the occurrence of such effects because ants can reduce the damage caused by a wide variety of herbivorous insects. Ants have been shown, in a previous field experiment, to decrease the damage caused by the pine weevil, Hylobius abietis (L.) (Coleoptera: Curculionidae), a pest species that causes high seedling mortality in forest regeneration areas. In this study, we specifically tested whether seedlings planted close to ant-attended seedlings experience associational resistance. We did this under laboratory conditions using the ant species Lasius niger (L.) (Hymenoptera: Formicidae). The feeding damage by pine weevils was significantly reduced on seedlings attended by ants. The neighbouring seedlings, however, did not experience associational resistance. Nevertheless, some associational effects were observed as the number of weevils recorded on both ant-attended and neighbouring seedlings was significantly lower compared with ant-excluded seedlings.
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| 49. |
- Manak, Vita, et al.
(författare)
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The effect of red wood ant abundance on feeding damage by the pine weevil Hylobius abietis
- 2015
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Ingår i: Agricultural and Forest Entomology. - : Wiley. - 1461-9555 .- 1461-9563. ; 17, s. 57-63
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Tidskriftsartikel (refereegranskat)abstract
- Red wood ants (Formica rufa group) are the dominant ant species in European forests that can affect the abundance of other arthropods (e.g. herbivorous insects in tree canopies or beetles on the forest floor). Notably, ants can prevent pine weevils (Hylobius abietis) from feeding on conifer seedlings supplied with food sources for ants. This is a potentially important observation because the weevil is a serious pest in forest regeneration areas. We hypothesized that frequent encounters between red wood ants and pine weevils on the ground may decrease the weevils' feeding on conifer seedlings. To test this hypothesis, we compared feeding damage caused by pine weevils on spruce seedlings in areas with high and low abundance of red wood ants. Despite a four-fold difference in the numbers of ants in pitfall trap samples, there were no significant differences between areas with high and low ant abundance in terms of feeding-scar areas, proportions of attacked seedlings or proportions of killed seedlings. Thus, in contrast to previously documented deterrent effects on ground-dwelling beetles, high abundance of ants on the ground did not influence the feeding activity of pine weevils on the spruce seedlings. We conclude that the mechanisms underlying seedling protection by ants are probably mainly related to the ants' protection of food sources, whereas the frequency of encounters elsewhere has less effect on the weevils' feeding.
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| 50. |
- Nordlander, Carola, 1969, et al.
(författare)
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Allelic imbalance on chromosome 10 in rat endometrial adenocarcinomas.
- 2005
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Ingår i: Cancer genetics and cytogenetics. - : Elsevier BV. - 0165-4608 .- 1873-4456. ; 156:2, s. 158-66
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Tidskriftsartikel (refereegranskat)abstract
- Earlier work using comparative genome hybridization (CGH) has shown that rat chromosome 10 (RNO10) is frequently involved in cytogenetic aberrations in BDII rat endometrial adenocarcinomas (EAC). Relative reduction in copy number (chromosomal deletions) was seen in the proximal to middle part of the chromosome, whereas there were increases in copy number in the distal part. The occurrence of RNO10 aberrations was further analyzed in DNA from primary tumor material from 42 EACs and 3 benign endometrial tumors using allelotyping of microsatellite markers. We found frequently that there were 4 quite distinct RNO10 regions that exhibited allelic imbalance. Based on these findings we believe that genes with relevance to EAC tumor development are situated in each of these chromosome regions. Extrapolation of our microsatellite marker data to the rat draft DNA sequence will facilitate the definition of the regions at the level of the DNA and to select and characterize candidate genes within each of the affected chromosome regions.
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