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1.	Ahrné, Karin, et al. (author) Rödlista över fjärilar Lepidoptera 2015 In: Rödlistade arter i Sverige 2015. - Uppsala : ArtDatabanken SLU. - 9789187853104 ; , s. 98-112 Book chapter (other academic/artistic)
2.	Arvidsson, Inger, et al. (author) Consequences on physical workload of rationalization in meat cutting. 2010 Conference paper (peer-reviewed)
3.	Arvidsson, Inger, et al. (author) Discrepancies in pain presentation caused by adverse psychosocial conditions as compared to pain due to high physical workload? 2012 In: Work: A Journal of Prevention, Assessment & Rehabilitation. - 1875-9270. ; 41, s. 2472-2475 Journal article (peer-reviewed)abstract Disorders in the musculoskeletal system have been associated with a high physical workload as well as psychosocial and individual factors. It is however not obvious which of these factors that is most important to prevent. Musculoskeletal disorders in neck and upper extremity was assessed by interview and clinical examination in 79 teachers and 93 assisting nurses, all females. Psychosocial work environment was assessed by questionnaire. The physical workload was recorded by technical measurements of postures, movements and muscular load, in 9 teachers and 12 nurses. The physical workload was lower among the teachers, but they had a more demanding psychosocial work environment. Among the nurses, but not in the teachers, the neck-shoulder disorders were associated with a high body mass index (BMI). The teachers reported neck-shoulder complaints to a higher extent than the nurses, but had much lower prevalence of diagnoses in the clinical examination (12% vs. 25%; POR 0.3 CI 0.1 - 1.2; adjusted for age and BMI). The results suggest that adverse psychosocial conditions among the teachers give rise to a different kind of pain in the neck-shoulder region than from physical overload, troublesome but not as severe as the one afflicting the nurses.
4.	Arvidsson, Inger, et al. (author) Rationalization in meat cutting - Consequences on physical workload. 2012 In: Applied Ergonomics. - : Elsevier BV. - 1872-9126 .- 0003-6870. ; 43:6, s. 1026-1032 Journal article (peer-reviewed)abstract Meat cutting is associated with several ergonomic risk factors and a high risk of musculoskeletal disorders. The development of new production systems points to an increased degree of mechanization; instead of subdividing split carcasses of pigs with a knife, the halves are trisected by an electrical saw into 'sixth-parts', resulting in shorter work cycles for the workers. Recently, machine-directed line-production systems have been implemented. This study evaluates differences in the physical workload between the production systems. The postures and movements (inclinometry and goniometry) and muscular load (electromyography) of workers in the split-carcass- (five subjects), sixth-part- (ten) and line-production systems (five) were recorded. Most measures showed a statistically significant trend of declining physical exposure with increasing degrees of mechanization. For example, movement velocities of the upper arm were higher in the split-carcass system (50th percentile: mean 209°/s) than in the sixth-part (103°/s) and line production (81°/s). However, the latter two were not statistically significantly different. A novel method for quantifying posture variation, based on inclinometry, showed that the split-carcass system implied the highest variation of the upper arm postures "within-minute" (i.e., a high range of motion each minute), but the lowest "between-minute" (i.e., a low variation during the course of the workday). In conclusion, the physical workload in the line-production system was significantly lower than in the split-carcass one, and tended also to be lower than in the sixth-part system. However, there may be disadvantages in line production, such as machine-directed work pace and shorter work cycles.
5.	Axelsson, Erik, et al. (author) The genetic consequences of dog breed formation-Accumulation of deleterious genetic variation and fixation of mutations associated with myxomatous mitral valve disease in cavalier King Charles spaniels 2021 In: PLOS Genetics. - : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 17:9 Journal article (peer-reviewed)abstract Selective breeding for desirable traits in strictly controlled populations has generated an extraordinary diversity in canine morphology and behaviour, but has also led to loss of genetic variation and random entrapment of disease alleles. As a consequence, specific diseases are now prevalent in certain breeds, but whether the recent breeding practice led to an overall increase in genetic load remains unclear. Here we generate whole genome sequencing (WGS) data from 20 dogs per breed from eight breeds and document a similar to 10% rise in the number of derived alleles per genome at evolutionarily conserved sites in the heavily bottlenecked cavalier King Charles spaniel breed (cKCs) relative to in most breeds studied here. Our finding represents the first clear indication of a relative increase in levels of deleterious genetic variation in a specific breed, arguing that recent breeding practices probably were associated with an accumulation of genetic load in dogs. We then use the WGS data to identify candidate risk alleles for the most common cause for veterinary care in cKCs-the heart disease myxomatous mitral valve disease (MMVD). We verify a potential link to MMVD for candidate variants near the heart specific NEBL gene in a dachshund population and show that two of the NEBL candidate variants have regulatory potential in heartderived cell lines and are associated with reduced NEBL isoform nebulette expression in papillary muscle (but not in mitral valve, nor in left ventricular wall). Alleles linked to reduced nebulette expression may hence predispose cKCs and other breeds to MMVD via loss of papillary muscle integrity.
6.	Balogh, Istvan, et al. (author) Hälsorisker, prevention och rehabilitering i gummiindustrin. Slutrapport 2003-03-06 2003 Reports (other academic/artistic)
7.	Balogh, Istvan, et al. (author) Increasing the Degree of Automation in a Production System: Consequences for the physical workload 2006 In: International Journal of Industrial Ergonomics. - : Elsevier BV. - 1872-8219 .- 0169-8141. ; 36:4, s. 353 - 365 Journal article (peer-reviewed)abstract In spite of the continuous development of production systems work-related musculoskeletal disorders is still a large problem. One reason might be the difficulties in quantifying the ergonomic effects of interventions. In this paper ergonomic consequences of technical and organisational changes were quantified in a plant for producing slats for parquet flooring. Muscle activity, work postures and movements were assessed for neck/shoulders and upper limb by direct technical measurements at three generations of production lines. The physical workload for 31 operators at the manual, semi-automated and automated line was derived based on all existing work tasks. The work was characterised by moderate muscular loads, the 50th percentiles being 1.2-3.8%MVE for the neck/shoulder muscles, high repetitiveness and constant movements of the hands and a high prevalence of neck/shoulder disorders. There were statistically significant differences considering exposure levels between the work tasks within each line as well as between the lines. The semi-automated line implied reduced muscular load for all muscles registered but more constrained work postures. The automated line, on the other hand, required higher skills, offered less constrained postures, lower loads and repetitiveness for the hands as well as frequent changes between different physical load levels. Relevance to industry: When designing or redesigning production systems, technical solutions are often used to obtain 'beneficial' ergonomic conditions, implying a strive for low physical workloads. However, there is a risk that this might lead to more constrained physical activities. Possibilities for job enlargement must be thoroughly utilised. © 2006 Elsevier B.V. All rights reserved.
8.	Balogh, Istvan, et al. (author) Physical workload in different work tasks in female grocery store workers. 2010 Conference paper (peer-reviewed)
9.	Balogh, Istvan, et al. (author) Precision of measurements of physical workload during standardized manual handling part III: Goniometry of the wrists. 2009 In: Journal of Electromyography & Kinesiology. - : Elsevier BV. - 1873-5711 .- 1050-6411. ; 19:5, s. 1005-1012 Journal article (peer-reviewed)abstract Goniometry of the wrist is a feasible method for studying wrist movements in most hand-intensive work. The precision and accuracy of the method per se is good. For the knowledge on validity of field measurements, the size of imprecision is of importance. This study evaluated this condition during standardized circumstances. Six women performed three different hand-intensive work tasks: 'materials picking', 'light assembly', and 'heavy assembly', repeated during three different days. Variance components between-days (within subjects) and between-subjects were derived for positions (flexion/extension and deviation) and movements, including angular velocities, % of time with very low velocity (<1 degrees /s), as well as repetitiveness. For positions, the average standard deviations in the three tasks were, both between-days and between-subjects, 3-4 degrees . For movements, the coefficients of variation of angular velocities were about 10% between-days, and could to a great part be explained by differences in work rate. Between-subjects variability was higher, 20-40%. The variability was larger at low velocities than at high ones. The precision of the measured positions was good, expressed as small between-days and between-subjects variability. For movements, the between-days variability was also small, while there was a larger between-subjects variability. The imprecision of goniometry is consequently lower and comparable with inclinometry but lower than for EMG.
10.	Balogh, Istvan, et al. (author) Self-assessed and directly measured occupational physical activities - influence of musculoskeletal complaints, age and gender 2004 In: Applied Ergonomics. - : Elsevier BV. - 1872-9126 .- 0003-6870. ; 35:1, s. 49-56 Journal article (peer-reviewed)abstract This study compares questionnaire assessed physical activity with direct technical measurements among cleaners and office workers, stratified regarding age, gender and self-reported neck/shoulder complaints. During two full working days number of steps was recorded by a pedometer, sitting/standing positions by a posimeter and heart rate by a Sport-Tester. In addition the subjects kept a work task diary for 10 days. There were high intra-individual variations in exposure between the days. Subjects with complaints rated their exposure higher than those without, although they in fact showed lower direct measured exposure. This may imply underestimation of exposure-effect relationships. Rate of perceived exertion showed low correlation with heart rate ratio within the two occupational groups, but high, 0.64 when the two groups were combined. Age and complaints explained 31% of the variance for the cleaners.
11.	Balogh, Istvan, et al. (author) The importance of work organization on workload and musculoskeletal health - Grocery store work as a model. 2016 In: Applied Ergonomics. - : Elsevier BV. - 1872-9126 .- 0003-6870. ; 53:Part A, s. 143-151 Journal article (peer-reviewed)abstract We have evaluated the consequences of work organization on musculoskeletal health. Using a postal questionnaire, answered by 1600 female grocery store workers, their main work tasks were identified and four work groups were defined (cashier, picking, and delicatessen work, and a mixed group, who performed a mix of these tasks). The crude odds ratios (ORs) for neck/shoulder complaints were 1.5 (95% CI 1.0-2.2), 1.1 (0.7-1.5) and 1.6 (1.1-2.3), respectively, compared to mixed work. Adjusting for individual and psychosocial factors had no effect on these ORs. For elbows/hands, no significant differences were found. Technical measurements of the workload showed large differences between the work groups. Picking work was the most strenuous, while cashier work showed low loads. Quantitative measures of variation revealed for mixed work high between minutes variation and the highest between/within minutes variation. Combining work tasks with different physical exposure levels increases the variation and may reduce the risk of musculoskeletal complaints.
12.	Balogh, Istvan, et al. (author) Work-related neck and upper limb disorders - Quantitative exposure-response relationships adjusted for personal characteristics and psychosocial conditions 2019 In: BMC Musculoskeletal Disorders. - : Springer Science and Business Media LLC. - 1471-2474. ; 20:1 Journal article (peer-reviewed)abstract Background: We have previously reported quantitative exposure-response relationships between physical exposures recorded by technical methods, and complaints and diagnoses in the neck/shoulders, and the elbows/hands, based on group data. In the present study the number of workers was doubled, and information on individual factors, and psychosocial working conditions was used. Relationships between various kinds of exposure and response have been analysed in this larger and more detailed sample. Methods: The prevalence of complaints (Nordic Questionnaire) and diagnoses (clinical examination) were recorded in a number of occupational groups within which the participants had similar work tasks, 34 groups of female employees (N = 4733 women) and 17 groups of male employees (N = 1107 men). Age and other individual characteristics were recorded, as well as psychosocial work environment factors (job-content questionnaire) for most participants. Postures and velocities (inclinometry) of the head (N = 505) and right upper arm (N = 510), right wrist postures and velocities (electrogoniometry; N = 685), and muscular activity (electromyography; EMG) in the right trapezius muscle (N = 647) and forearm extensors (N = 396) were recorded in representative sub-groups. Exposure-response relationships between physical exposure and musculoskeletal disorders, adjusted for individual factors with Poisson regression were then calculated. The effect of introducing psychosocial conditions into the models was also assessed. Results: Associations were found between head velocity, trapezius activity, upper arm velocity, forearm extensor activity and wrist posture and velocity, and most neck/shoulder and elbow/hand complaints and diagnoses. Adjustment for age, other individual characteristics and psychosocial work conditions had only a limited effect on these associations. For example, the attributable fraction for tension neck syndrome among female workers with the highest quintile of trapezius activity was 58%, for carpal tunnel syndrome versus wrist velocity it was 92% in men in the highest exposure quintile. Conclusions: Based on the findings, we propose threshold limit values for upper arm and wrist velocity.
13.	Christmansson, M, et al. (author) A case study of a principally new way of materials kitting - an evaluation of time consumption and physical workload 2002 In: International Journal of Industrial Ergonomics. - : Elsevier. - 0169-8141. ; 30:1, s. 49-65 Journal article (peer-reviewed)abstract This article presents a materials kitting case study, using an alternative method for materials killing: the traditional picker-to-material principle was replaced with a material-to-picker approach. The Study was made in a materials supply workshop of an automobile plant shortly after this kitting process had been introduced. The materials kitting was video recorded and the material pickers' physical exposure was assessed using ambulatory equipment to make an integrated registration of muscular activity, work postures and movements. The material pickers assessed work situations perceived as physically stressful. The materials kitting showed improved productivity as compared with other kitting methods. The study indicated that the work situation offered pickers low levels of physical exposure. The picking work was rather light but involved great repetitiveness in arm movements. The material pickers experienced the work as repetitive and having some physically stressful work situations. The integrated analyses showed: (1) that exposure in picking operations was similar to, but could be distinguished from, other work activities and (2) that picking from one type of storage package, Euro pallets, resulted in higher exposure than picking from plastic containers. More studies are needed on the implications of exposure levels in materials picking.
14.	Christmansson, Marita, et al. (author) A case study of a principally new way of materials kitting—an evaluation of time consumption and physical workload 2002 In: International Journal of Industrial Ergonomics. - : Elsevier. - 0169-8141 .- 1872-8219. ; 30:1, s. 49-65 Journal article (peer-reviewed)abstract This article presents a materials kitting case study, using an alternative method for materials kitting: the traditional picker-to-material principle was replaced with a material-to-picker approach. The study was made in a materials supply workshop of an automobile plant shortly after this kitting process had been introduced. The materials kitting was video recorded and the material pickers’ physical exposure was assessed using ambulatory equipment to make an integrated registration of muscular activity, work postures and movements. The material pickers assessed work situations perceived as physically stressful. The materials kitting showed improved productivity as compared with other kitting methods. The study indicated that the work situation offered pickers low levels of physical exposure. The picking work was rather light but involved great repetitiveness in arm movements. The material pickers experienced the work as repetitive and having some physically stressful work situations. The integrated analyses showed: (1) that exposure in picking operations was similar to, but could be distinguished from, other work activities and (2) that picking from one type of storage package, Euro pallets, resulted in higher exposure than picking from plastic containers. More studies are needed on the implications of exposure levels in materials picking.
15.	Cronholm, Felix, et al. (author) The fracture predictive ability of a musculoskeletal composite score in old men - data from the MrOs Sweden study 2019 In: BMC Geriatr. - : Springer Science and Business Media LLC. - 1471-2318. ; 19:1 Journal article (peer-reviewed)abstract BackgroundDetection of high-risk individuals for fractures are needed. This study assessed whether level of physical activity (PA) and a musculoskeletal composite score could be used as fracture predictive tools, and if the score could predict fractures better than areal bone mineral density (aBMD).MethodsMrOs Sweden is a prospective population-based observational study that at baseline included 3014 men aged 69-81years. We assessed femoral neck bone mineral content (BMC), bone area, aBMD and total body lean mass by dual energy X-ray absorptiometry, calcaneal speed of sound by quantitative ultrasound and hand grip strength by a handheld dynamometer. PA was assessed by the Physical Activity Scale for the Elderly (PASE) questionnaire. We followed the participants until the date of first fracture, death or relocation (median 9.6years). A musculoskeletal composite score was calculated as mean Z-score of the five measured traits. A Cox proportional hazards model was used to analyze the association between the musculoskeletal traits, the composite score and incident fractures (yes/no) during the follow-up period. Data are presented as hazard ratios (HR) with 95% confidence intervals (95% CI) for fracture for a+1 standard deviation (SD) change (+1 Z-score) in the various musculoskeletal traits as well as the composite score. We used a linear regression model to estimate the association between level of PA, measured as PASE-score and the different musculoskeletal traits as well as the composite score.ResultsA+1 SD higher composite score was associated with an incident fracture HR of 0.61 (0.54, 0.69), however not being superior to aBMD in fracture prediction. A+1 SD higher PASE-score was associated with both a higher composite score and lower fracture incidence (HR 0.83 (0.76, 0.90)).ConclusionsThe composite score was similar to femoral neck aBMD in predicting fractures, and also low PA predicted fractures. This highlights the need of randomized controlled trials to evaluate if PA could be used as a fracture preventive strategy.
16.	Dahlström, Ulf, 1946-, et al. (author) Hjärtsvikt hos äldre 2001 In: Nordisk geriatrik. - 1403-2082. ; 1, s. 30-36 Journal article (other academic/artistic)
17.	Drotz, Marcus, 1968, et al. (author) Forskning och kunskapsproducenter runt Vänern. Kunskapsunderlag inom Vänersamarbetet 2012 Reports (other academic/artistic)abstract Rapporten är en kunskaps- och forskningsinventering av befintlig och avslutad forskning, samt kunskapsproducenter med anknytning till Vänern. Geografiskt har inventeringen begränsats till kommunerna i anslutning till Vänern samt vattnet och dess tillflöden, utflöde och kanaler. Ämnesmässigt har inventeringen fokuserat på forskning och kunskapsproduktion som direkt rör Vänerområdet inom såväl natruvetenskap, samhällsvetenskap som humaniora, speciellt det som rör arbetet inom Vänersamarbetet som är relaterat till hållbar utveckling socialt, ekonomiskt och ekologiskt. Tidsmässigt har arbetet fokuserats på att sammanställa projekt som pågår eller är avslutade under de senaste fem åren. Genom denna avgränsning har vi i mindre utsträckning tagit med forskning, kunskapsproduktion, nätverk och myndighetsdokumentation som rör större regionala områden eller Sverige i stort. Målgruppen är forskare, tjänstemän och beslutsfattare inom den offentliga sektorn. Projektet har genomförts i samarbete med Västra Götalandsregionen och Region Värmland. Till rapporten hör en referenssamling i form av en bilaga, ISBN: 91-88048-18-7.
18.	Duan, Rui-Dong, et al. (author) Evidence for specific ceramidase present in the intestinal contents of rats and humans. 2001 In: Lipids. - : Wiley. - 0024-4201 .- 1558-9307. ; 36:8, s. 807-812 Journal article (peer-reviewed)abstract A neutral ceramidase activity stimulated by bile salt was previously identified in the intestinal content. Recently, bile salt stimulated lipase (BSSL) was found to have ceramidase activity. It is unknown whether the ceramidase activity previously found is attributable to BSSL. To address this question, we compared the behaviors of high quaternary aminoethyl (HQ) anion exchange chromatography, the distributions, the stability, and the responses to lipase inhibitor between ceramidase and pancreatic BSSL. The proteins from whole small intestinal contents of humans and rats were precipitated by acetone and dissolved in 20 mM Tris buffer pH 8.2. These proteins had neutral ceramidase activity but not BSSL activity against p‐nitrophenyl acetate. When the proteins were subject to HQ chromatography, two peaks of ceramidase activity were identified, which had acid and neutral pH optima, respectively. Neither of them had BSSL activity against p‐nitrophenyl acetate. Western blot using BSSL antiserum failed to identify BSSL protein in the fractions, with high neutral ceramidase activity. In rat intestinal tract, pancreatic BSSL activity was high in the duodenum and declined rapidly in the small intestine, whereas neutral ceramidase activity was low in the duodenum and maintained a high level until the distal part of the small intestine. In addition, orlistat, the inhibitor of lipase, abolished human BSSL activity against p‐nitrophenyl acetate and slightly reduced its activity against ceramide but had no inhibitory effect on ceramidase activity isolated by HQ chromatography. In conclusion, we provide the evidence for a specific ceramidase other than pancreatic BSSL present in the intestinal content. The enzyme may play important roles in digestion of dietary sphingolipids.
19.	Duan, Rui-Dong, et al. (author) Human meconium contains significant amounts of alkaline sphingomyelinase, neutral ceramidase, and sphingolipid metabolites. 2007 In: Pediatric Research. - : Springer Science and Business Media LLC. - 1530-0447 .- 0031-3998. ; 61:1, s. 61-66 Journal article (peer-reviewed)abstract Intestinal alkaline sphingomyelinase (Alk-SMase) and neutral ceramidase may catalyze the hydrolysis of endogenous sphin-gomyelin (SM) and milk SM in human-milk fed infants. The enzymes generate sphingolipid metabolites that may influence gut maturation. Alk-SMase also inactivates platelet-activating factor (PAF) that is involved in the pathogenesis of necrotizing enterocolitis (NEC). We examined whether the two enzymes are expressed in both preterm and term infants and analyzed Alk-SMase, neutral ceramidase, SM, and sphingolipid metabolites in meconium. Meconium was collected from 46 preterm (gestational ages 23-36 wk) and 38 term infants (gestational ages 37-42 wk) and analyzed for Alk-SMase using C-14-choline-labeled SM and for neutral ceramidase using C-14-octanoyl-sphingosine as substrates. Molecular species of SM, ceramide, and sphingosine were analyzed by high-performance liquid chromatography mass spectroscopy. Meconium contained significant levels of Alk-SMase and ceramidase at all gestational ages. It also contained 16-24 carbon molecular species of SM, palmitoyl-and stearoyl-sphingosine, and sphingosine. There were positive correlations between levels of SM and ceramide and between ceramide and sphingosine levels. In conclusion, Alk-SMase and ceramidase are expressed in the gut of both preterm and term newborn infants and may generate bioactive sphingolipid messengers.
20.	Ehrnborg, Christer, 1968, et al. (author) Increased serum concentration of IGFBP-4 and IGFBP-5 in healthy adults during one month's treatment with supraphysiological doses of growth hormone. 2007 In: Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society. - : Elsevier BV. - 1096-6374. ; 17:3, s. 234-41 Journal article (peer-reviewed)abstract OBJECTIVES: To study the effects on insulin-like growth factor binding proteins (IGFBP)-4 and -5 after one month's treatment with supraphysiological doses of growth hormone (GH) in healthy, active young adults with a normal GH-IGF-I axis. Furthermore, the possible use of IGFBP-4 and IGFBP-5 as markers of GH doping is discussed. DESIGN: Thirty healthy, physically active volunteers (15 men and 15 women), mean age 25.9 years (range 18-35), participated in this randomized, double-blind, placebo-controlled, parallel study with three groups (n=10; 5 men and 5 women in each group). The groups comprised the following: placebo, GH 0.1IU/kg/day [0.033mg/kg/day] and GH 0.2IU/kg/day [0.067mg/kg/day]. RESULTS: Baseline levels of IGFBP-4 were higher (+20%), while IGFBP-5 levels were lower (-37%) in women than in men. IGFBP-5 levels were positively correlated to age, but no significant correlation was found for IGFBP-4. In the pooled group with active GH treatment (n=20), both IGFBP-4 and IGFBP-5 levels were increased vs. the placebo group from day 14 until end of treatment [day 28, IGFBP-4 (+40%, p<0.01) and IGFBP-5 (+61%, p <0.001)]. After inclusion of serum IGF-I as a covariate in the linear regression analysis, the associations between GH treatment and the IGFBP-4 and IGFBP-5 levels were not significant. CONCLUSIONS: This study shows that the levels of IGFBP-4 and IGFBP-5 are affected by supraphysiological GH treatment given to young, healthy, physically active adults of both genders. The present study, including relatively few subjects, does not support that IGFBP-4 and IGFBP-5 can be used as IGF-I independent markers in a forthcoming method for detecting GH doping, although, further studies are needed to investigate the potential use of IGFBP-4 and IGFBP-5 as markers of GH doping.
21.	Emek, Sinan Cem, et al. (author) Pigments protect the light harvesting proteins of chloroplast thylakoid membranes against digestion by gastrointestinal proteases 2011 In: Food Hydrocolloids. - : Elsevier BV. - 0268-005X. ; 25:6, s. 1618-1626 Journal article (peer-reviewed)abstract Chloroplast thylakoid membranes inhibit pancreatic lipase/colipase activity in vitro and, when included in food, induce satiety signals. As thylakoid membranes themselves are nutrients, containing lipids and proteins, it is of interest to study the digestion of thylakoids by enzymes of the gastrointestinal tract. Thylakoid membranes were treated with pepsin, trypsin, gastric and pancreatic juice at 37 degrees C and the resulting enzymatic breakdown was analyzed by gel electrophoresis, electron microscopy and mass spectroscopy. In all cases, several of the proteins were degraded within half an hour, while the main parts of the pigment-protein complexes were resistant for hours. Oil emulsified thylakoids were more resistant towards the enzymatic breakdown. Electron microscopy demonstrated that, after treatments, the thylakoids still remained in a membrane vesicular form. The capacity of thylakoid membranes to inhibit the lipase/colipase activity was partly reduced in all cases. About 50% of the inhibition capacity remained after treatment with pancreatic juice when the thylakoids were present in an oil emulsion. Delipidated thylakoids and plasma membranes, which lack the photosynthetic pigments, were degraded rapidly by pancreatic juice. Conclusion: The pigments, closely bound to the trans-membrane helices of thylakoid membrane proteins protect these from digestion by pepsin, trypsin, gastric and pancreatic juice. This supports the notion that a substantial inhibition of lipase/colipase takes place during the first 2 h in the intestine resulting in a retardation and prolongation of lipolysis in vivo. (C) 2010 Elsevier Ltd. All rights reserved.
22.	Engström, Tomas, 1950, et al. (author) Technical and Human Aspects of Welding Work - Analysis of Efficiency and Ergonomics of Two Work Stations 1999 Conference paper (other academic/artistic)abstract This publication was written during a period of time when the authors were engaged in an extensive research programme financed by the National Institute for Working Life in Solna (Cooperative for Optimisation of industrial production systems regarding Productivity and Ergonomics COPE). And, this publication deals with welding and welding work with regard to specific time consumption and particular ergonomic aspects, as was practised within the Autonova plant in Uddevalla (i.e. the successor of the Volvo Uddevalla plant reopened some years later).
23.	Feng, Dan, et al. (author) Generating Ceramide from Sphingomyelin by Alkaline Sphingomyelinase in the Gut Enhances Sphingomyelin-Induced Inhibition of Cholesterol Uptake in Caco-2 Cells. 2010 In: Digestive Diseases and Sciences. - : Springer Science and Business Media LLC. - 1573-2568 .- 0163-2116. ; Mar 4, s. 3377-3383 Journal article (peer-reviewed)abstract BACKGROUND: Sphingomyelin (SM) is present in dietary products and cell plasma membranes. We previously showed that dietary SM inhibited cholesterol absorption in rats. In the intestinal tract, SM is mainly hydrolyzed by alkaline sphingomyelinase (alk-SMase) to ceramide. AIMS: We investigated the influence of SM and its hydrolytic products ceramide and sphingosine on cholesterol uptake in intestinal Caco-2 cells. METHODS: Micelles containing bile salt, monoolein, and (14)C-cholesterol were prepared with or without SM, ceramide, or sphingosine. The micelles were incubated with Caco-2 cells, and uptake of radioactive cholesterol was quantified. RESULTS: We found that confluent monolayer Caco-2 cells expressed NPC1L1, and the uptake of cholesterol in the cells was inhibited by ezetimibe, a specific inhibitor of NPC1L1. Incorporation of SM in the cholesterol micelles inhibited cholesterol uptake dose-dependently; 38% inhibition occurred at an equal mole ratio of SM and cholesterol. The inhibition was further enhanced to 45% by pretreating the cholesterol/SM micelles with recombinant alk-SMase, which hydrolyzed SM in the micelles by 85%, indicating ceramide has stronger inhibitory effects on cholesterol uptake. To confirm this, we further replaced SM in the micelles with ceramide and sphingosine, and found that at equal mole ratio to cholesterol, ceramide exhibited stronger inhibitory effect (50% vs 38%) on cholesterol uptake than SM, whereas sphingosine only had a weak effect at high concentrations. CONCLUSION: Both SM and ceramide inhibit cholesterol uptake, the effect of ceramide being stronger than that of SM. Alk-SMase enhances SM-induced inhibition of cholesterol uptake by generating ceramide in the intestinal lumen.
24.	Forsblad d'Elia, Helena, 1961, et al. (author) Hormone replacement therapy, calcium and vitamin D3 versus calcium and vitamin D3 alone decreases markers of cartilage and bone metabolism in rheumatoid arthritis: a randomized controlled trial [ISRCTN46523456] 2004 In: Arthritis Res Ther. - : Springer Science and Business Media LLC. - 1478-6362 .- 1465-9905 .- 1478-6354. ; 6:5 Journal article (peer-reviewed)abstract This study aimed to evaluate the effects of hormone replacement therapy (HRT), known to prevent osteoporosis and fractures, on markers of bone and cartilage metabolism. Furthermore, we assessed whether changes in these markers corresponded to alterations in bone mineral density and radiographic joint destructions in postmenopausal women with rheumatoid arthritis. Eighty-eight women were randomized to receive HRT, calcium, and vitamin D3, or calcium and vitamin D3 alone, for 2 years. Bone turnover was studied by analyzing serum levels of C-terminal telopeptide fragments of type I collagen (CTX-I), C-terminal telopeptide of type I collagen (ICTP), bone sialoprotein, and C-terminal propeptide of type I procollagen (PICP) and cartilage turnover by urinary levels of collagen type II C-telopeptide degradation fragments (CTX-II) and cartilage oligomeric matrix protein (COMP) in serum. Treatment with HRT resulted in decrease in CTX-I (P < 0.001), ICTP (P < 0.001), PICP (P < 0.05), COMP (P < 0.01), and CTX-II (P < 0.05) at 2 years. Reductions in CTX-I, ICTP, and PICP were associated with improved bone mineral density. Of the markers tested, CTX-I reflected bone turnover most sensitively; it was reduced by 53 +/- 6% in the patients receiving HRT. Baseline ICTP (P < 0.001), CTX-II (P < 0.01), and COMP (P < 0.05) correlated with the Larsen score. We suggest that biochemical markers of bone and cartilage turnover may provide a useful tool for assessing novel treatment modalities in arthritis, concerning both joint protection and prevention of osteoporosis.
25.	Forsblad d'Elia, Helena, 1961, et al. (author) Hormone replacement therapy in rheumatoid arthritis is associated with lower serum levels of soluble IL-6 receptor and higher insulin-like growth factor 1. 2003 In: Arthritis research & therapy. - : Springer Science and Business Media LLC. - 1478-6362 .- 1465-9905 .- 1478-6354. ; 5:4 Journal article (peer-reviewed)abstract Hormone replacement therapy (HRT) modulates the imbalance in bone remodeling, thereby decreasing bone loss. Sex hormones are known to influence rheumatic diseases. The aim of this study was to investigate the effects of HRT on the serum levels of hormones and cytokines regulating bone turnover in 88 postmenopausal women with active rheumatoid arthritis (RA) randomly allocated to receive HRT plus calcium and vitamin D3 or calcium and vitamin D3 alone for 2 years. An increase in estradiol (E2) correlated strongly with improvement of bone mineral density in the hip (P < 0.001) and lumbar spine (P < 0.001). Both baseline levels and changes during the study of IL-6 and erythrocyte sedimentation rate were correlated positively (P < 0.001). HRT for 2 years resulted in an increase of the bone anabolic factor, insulin-like growth factor 1 (IGF-1) (P < 0.05) and a decrease of serum levels of soluble IL-6 receptor (sIL-6R) (P < 0.05), which is known to enhance the biological activity of IL-6, an osteoclast-stimulating and proinflammatory cytokine. Baseline levels of IL-6 and IGF-1 were inversely associated (P < 0.05), and elevation of IGF-1 was connected with decrease in erythrocyte sedimentation rate (P < 0.05) after 2 years. Interestingly, increase in serum levels of E2 was associated with reduction of sIL-6R (P < 0.05) and reduction of sIL-6R was correlated with improved bone mineral density in the lumbar spine (P < 0.05). The latter association was however not significant after adjusting for the effect of E2 (P = 0.075). The influences of IGF-1 and the IL-6/sIL-6R pathways suggest possible mechanisms whereby HRT may exert beneficial effects in RA. However, to confirm this hypothesis future and larger studies are needed.
26.	Forsblad d'Elia, Helena, 1961, et al. (author) Influence of hormone replacement therapy on disease progression and bone mineral density in rheumatoid arthritis. 2003 In: The Journal of rheumatology. - 0315-162X .- 1499-2752. ; 30:7, s. 1456-63 Journal article (peer-reviewed)abstract OBJECTIVE: Hormone replacement therapy (HRT) is known to exert a positive effect in preventing bone loss and a beneficial effect on the disease activity in rheumatoid arthritis (RA). We evaluated the effects of HRT on bone mineral density (BMD) and on the course of established RA. METHODS: Eighty-eight postmenopausal women with RA were randomly allocated to receive HRT, vitamin D3, and calcium supplementation or vitamin D3 and calcium supplementation alone for 2 years. The effects of additional HRT on laboratory and clinical measures of disease activity, quality of life, and BMD and on radiographic joint damage were investigated. RESULTS: Treatment with HRT suppressed signs of inflammation as shown by reduction in erythrocyte sedimentation rate (ESR) (p = 0.025) and an elevation in hemoglobin concentration (p = 0.007), a better clinical outcome assessed by response on the Disease Activity Score 28 (DAS28) (p = 0.036), increased BMD in the forearm, proximal femur and spine (p < 0.01), and retarded (p = 0.026) progression of joint destruction among patients with radiological progressive disease. No significant effect on quality of life was seen. CONCLUSION: Two years of HRT in women with active RA had significant ameliorating effects on inflammation, DAS28 response, and BMD and was associated with slower progression of radiological joint destruction. The mechanisms by which HRT exerts its effects remain to be elucidated. We suggest HRT can be used in addition to conventional therapy in the management of postmenopausal patients with RA.
27.	Ghali, Jalal K, et al. (author) The influence of renal function on clinical outcome and response to beta-blockade in systolic heart failure: insights from Metoprolol CR/XL Randomized Intervention Trial in Chronic HF (MERIT-HF). 2009 In: Journal of cardiac failure. - : Elsevier BV. - 1532-8414 .- 1071-9164. ; 15:4, s. 310-8 Journal article (peer-reviewed)abstract BACKGROUND: Limited information is available on the risk and impact of renal dysfunction on the response to beta-blockade and mode of death in systolic heart failure (HF). METHODS AND RESULTS: Renal function was estimated with glomerular filtration rate (eGFR) using the simplified Modification of Diet in Renal Disease (MDRD) equation. Patients from the Metoprolol CR/XL Controlled Randomized Intervention Trial in Chronic HF (MERIT-HF) were divided into 3 renal function subgroups (MDRD formula): eGFR(MDRD) > 60 (n = 2496), eGFR(MDRD) 45 to 60 (n = 976), and eGFR(MDRD) < 45 mL/min per 1.73 m(2) body surface area (n = 493). Hazard ratio (HR) was estimated with Cox proportional hazards models adjusted for prespecified risk factors. Placebo patients with eGFR < 45 had significantly higher risk than those with eGFR > 60: HR for all-cause mortality, 1.90 (95% confidence interval [CI], 1.28 to 2.81) comparing placebo patients with eGFR < 45 and eGFR > 60, and for the combined end point of all-cause mortality/hospitalization for worsening HF (time to first event): HR, 1.91 (95% CI, 1.44 to 2.53). No significant increase in risk with deceased renal function was observed for those randomized to metoprolol controlled release (CR)/extended release (XL) due to a highly significant decrease in risk on metoprolol CR/XL in those with eGFR < 45. For total mortality, metoprolol CR/XL vs placebo: HR, 0.41 (95% CI. 0.25 to 0.68; P < .001) in those with eGFR < 45 compared with HR, 0.71 (95% CI, 0.54 to 0.95; P < .021) for those with eGFR > 60; corresponding data for the combined end point was HR, 0.44 (95% CI, 0.31 to 0.63; P < .0001) and HR, 0.75 (0.62 to 0.92; P = .005, respectively; P = .095 for interaction by treatment for total mortality; P = .011 for combined end point). Metoprolol CR/XL was well tolerated in all 3 renal function subgroups. CONCLUSIONS: Renal function as estimated by eGFR was a powerful predictor of death and hospitalizations from worsening HF. Metoprolol CR/XL was at least as effective in reducing death and hospitalizations for worsening HF in patients with eGFR < 45 as in those with eGFR > 60.
28.	Ghanei, Iman, et al. (author) The prevalence and severity of low back pain and associated symptoms in 3,009 old men 2014 In: European spine journal. - : Springer Science and Business Media LLC. - 0940-6719 .- 1432-0932. ; 23:4, s. 814-820 Journal article (peer-reviewed)abstract The aim of this study is to evaluate the prevalence and severity of low back pain (LBP) and the influence of sciatica and neurological deficits in old men. Mister osteoporosis Sweden includes 3,014 community-dwelling men aged 69-81 years. At study start 3,009 participants answered questions on LBP, low back pain and sciatica (LBP + SCI) or low back pain and sciatica with associated neurological deficits (LBP + SCI + NEU) during the preceding 12 months. Data are presented as proportions or medians with mid-quartile ranges. Differences between groups were tested by chi(2) test and Kruskall-Wallis test. 24 % had experienced LBP without SCI, 8 % LBP + SCI and 14 % LBP + SCI + NEU. 10 % of the men with LBP, 22 % of those with LBP + SCI, and 36 % of those with LBP + SCI + NEU rated the pain as severe (p < 0.001). 23 % of the men with LBP, 31 % of those with LBP + SCI and 50 % of those with LBP + SCI + NEU reported limitation in activity of daily living (ADL) (p < 0.001). Men with only LBP had to restrict their activities for 7 days (3-14), those with LBP + SCI 6 days (2-14) and those with LBP + SCI + NEU 10 days (3-30) (p < 0.05). The 1-year prevalence of LBP in community living men aged 69-81 years was close to 50 % but for individuals with LBP or LBP + SCI the morbidity was low with more than two-thirds having no limitations in ADL. In men with LBP + SCI + NEU more than one-third rated the pain as severe and close to half had limitations in ADL.
29.	Hansson, Gert-Åke, et al. (author) Exposure-response relation for wrist/hand disorders 2004 In: Premus 2004. Conference paper (peer-reviewed)
30.	Hansson, Gert-Åke, et al. (author) Measurements of wrist and forearm positions and movements: effect of, and compensation for, goniometer crosstalk. 2004 In: Journal of Electromyography & Kinesiology. - : Elsevier BV. - 1873-5711 .- 1050-6411. ; 14:3, s. 355-367 Journal article (peer-reviewed)
31.	Hansson, Gert-Åke, et al. (author) Physical workload in various types of work: Part I. Wrist and forearm 2009 In: International Journal of Industrial Ergonomics. - : Elsevier BV. - 0169-8141. ; 39:1, s. 221-233 Journal article (peer-reviewed)abstract The quantitative relationship between exposure to physical risk factors and upper extremity work-related musculoskeletal disorders (UE-WMSDs) is virtually unknown. To explore the variation, objective measurements were derived in 43 types of work (686 individuals), using goniometry for the wrists and electromyography (EMG) for the forearm extensor muscles. The variations due to work were great for wrist movements, wrist positions, muscular rest, as well as peak load, ranging 1.4-54 degrees/s (flexion velocity; 50th percentile), -30 degrees-3 degrees (flexion angle; 50th percentile), 0.2-23% of time, and 3.4-41% of maximal EMG (90th percentile), respectively. Even within work categories, e.g. "repetitive industrial", there were large variations for all measures. Hence, classification without measurements has limited value. All movement measures were highly correlated (vertical bar r(s)vertical bar=0.82-0.99), but only weakly so to positions (vertical bar r(s)vertical bar = 0.01-0.43). Muscular rest and "static load" (10th percentile), were highly correlated (r(s) = -0.92), but not associated to peak load (90th percentile; vertical bar r(s)vertical bar= 0.05 and 0.08, respectively). Most low-velocity work was accompanied by much muscular rest; however, the low velocity for mouse-intensive computer-work meant very little rest. Technical measurements are suitable as exposure measures in epidemiological studies, as well as a base for decisions about interventions. The multidimensional character of exposure - wrist movements, wrist postures, muscular recovery, and peak load - has to be considered. Relevance to industry: Direct measurements provide objective and quantitative measures of the main physical risk factors for UE-WMSDs, appropriate for estimating the risk, as well as giving priority to, and evaluating, interventions. (c) 2008 Elsevier B.V. All rights reserved.
32.	Hansson, Gert-Åke, et al. (author) Physical workload in various types of work: Part II. Neck, shoulder and upper arm 2010 In: International Journal of Industrial Ergonomics. - : Elsevier BV. - 0169-8141. ; 40:3, s. 267-281 Journal article (peer-reviewed)abstract To explore the correlation between, and the variation in, various measures of exposure to potential risk factors for work-related upper extremity musculoskeletal disorders (UE-WMSDs), physical workload was measured in 43 types of work (713 individuals), using inclinometry for the head and upper arms, and electromyography (EMG) for the trapezius muscles. Many exposure measures were highly correlated. Head flexion (90th percentile), extension (1st percentile), and movements (50th percentile); arm elevation (99th percentile) and movements (50th percentile); trapezius muscular rest (fraction of time) and peak load (90th percentile), constitute main exposure dimensions. The variations were large: head: flexion 9 degrees-63 degrees, extension -39 degrees-4 degrees, movements 2.3-33 degrees/s; arm: elevation 49 degrees-124 degrees, movements 3.0-103 degrees/s; trapezius: muscular rest 0.8%-52% of time, peak load 3.1%-24% of maximal EMC. Even within work categories, e.g. "repetitive industrial", there were large variations. Somewhat higher loads were recorded on the right as compared to the left side (differences: arm elevation 2, arm movements 19%; trapezius peak load 18%), but these were small compared to the differences due to work. There were high correlations between movements of arm and head (r(s) = 0.96), as well as arm and wrist (r(s) = 0.92), and between, on the one hand, trapezius muscular rest and peak load, and on the other, arm and head movements (vertical bar r(s)vertical bar = 0.47-0.62), as well as arm elevation (vertical bar r(s)vertical bar = 0.54-0.85), which has to be considered when assessing exposure-response relations. Relevance to industry: Direct measurements provide objective and quantitative data of the main physical risk factors for UE-WMSDs, appropriate for estimating the risk, as well as giving priority to and evaluating interventions. (C) 2009 Elsevier B.V. All rights reserved.
33.	Hansson, Gert-Åke, et al. (author) Physical workload on neck, shoulder and upper arm in various types of work. 2010 Conference paper (peer-reviewed)
34.	Hansson, Gert-Åke, et al. (author) Physical workload on wrist and forearm in various types of work 2007 In: Premus 2007. Conference paper (other academic/artistic)
35.	Hansson, Gert-Åke, et al. (author) Precision of measurements of physical workload during standardised manual handling. Part II: Inclinometry of head, upper back, neck and upper arms 2006 In: Journal of Electromyography & Kinesiology. - : Elsevier BV. - 1050-6411 .- 1873-5711. ; 16:2, s. 125-136 Journal article (peer-reviewed)abstract For measuring the physical exposure/workload in studies of work-related musculoskeletal disorders, direct measurements are valuable. However, the between-days and between-subjects variability, as well as the precision of the method per se, are not well known. In a laboratory, six women performed three standardised assembly tasks, all of them repeated on three different days. Triaxial inclinometers were applied to the head, upper back and upper arms. Between-days (within subjects) and between-subjects (within tasks) variance components were derived for the 10th, 50th and 90th percentiles of the angular and the angular velocity distributions, and for the proportion of time spent in predefined angular sectors. For percentiles of the angular distributions, the average between-days variability was 3.4 degrees , and the between-subjects variability 4.0 degrees . For proportion of time spent in angular sectors, the variability depended on the percentage of time spent in the sector; the relative variability was scattered and large, on average 103% between days and 56% between subjects. For the angular velocity percentiles, the average between-days variability was 7.9%, and the average between-subjects variability was 22%. The contribution of the measurement procedure per se to the between-days variability, i.e., the imprecision of the method, was small: less than 2 degrees for angles and 3% for angular velocity.
36.	Hansson, Gert-Åke, et al. (author) Questionnaire versus direct technical measurements in assessing postures and movements of the head, upper back, arms and hands 2001 In: Scandinavian Journal of Work, Environment and Health. - 0355-3140. ; 27:1, s. 30-40 Journal article (peer-reviewed)abstract OBJECTIVES: This study compares questionnaire-assessed exposure data on work postures and movements with direct technical measurements. METHODS: Inclinometers and goniometers were used to make full workday measurements of 41 office workers and 41 cleaners, stratified for such factors as musculoskeletal complaints. The subjects answered a questionnaire on work postures of the head, back, and upper arms and repeated movements of the arms and hands (3-point scales). The questionnaire had been developed on the basis of a previously validated one. For assessing worktasks and their durations, the subjects kept a 2-week worktask diary. Job exposure was individually calculated by time-weighting the task exposure measurements according to the diary. RESULTS: The agreement between the self-assessed and measured postures and movements was low (kappa = 0.06 for the mean within the occupational groups and kappa = 0.27 for the whole group). Cleaners had a higher measured workload than office workers giving the same questionnaire response. Moreover, the subjects with neck-shoulder complaints rated their exposure to movements as higher than those without complaints but with the same measured mechanical exposure. In addition, these subjects also showed a general tendency to rate their postural exposure as higher. The women rated their exposure higher than the men did. CONCLUSIONS: The questionnaire-assessed exposure data had low validity. For the various response categories the measured exposure depended on occupation. Furthermore, there was a differential misclassification due to musculoskeletal complaints and gender. Thus it seems difficult to construct valid questionnaires on mechanical exposure for establishing generic exposure-response relations in epidemiologic studies, especially cross-sectional ones. Direct technical measurements may be preferable.
37.	Hansson, Gert-Åke, et al. (author) Sensitivity of trapezius electromyography to differences between work tasks - influence of gap definition and normalisation methods 2000 In: Journal of Electromyography & Kinesiology. - 1873-5711. ; 10:2, s. 103-115 Journal article (peer-reviewed)abstract Surface electromyography (EMG) has been used extensively to estimate muscular load in studies of work related musculoskeletal disorders, especially for the trapezius muscle. The occurrences of periods of EMG silence (gaps), the time below a predetermined threshold level (muscular rest) and various percentiles of the amplitude distribution (APDF) are commonly used summary measures. However, the effects of the criteria used to calculate these measures (e.g., gap duration, threshold level, normalisation method) on the sensitivity of these measures to accurately differentiate work loads is not well known.Bilateral trapezius EMG was recorded, for a full workday, for 58 subjects following both maximal (MVE) and submaximal (RVE) reference contractions. Gap frequency, muscular rest, and percentiles were derived for eight fundamental work tasks. The calculations were performed using different gap duration criteria, threshold levels and normalisation methods.A gap duration of less than 1/2 s, and threshold level approximately 0.3% MVE for gap frequency, and approximately 0.5% MVE for muscular rest, were the criteria that optimised sensitivity to task differences. Minimal sensitivity to tasks and a high sensitivity to individuals was obtained using gap frequency with a threshold level of approximately 1% MVE. Normalisation to RVE, rather than MVE, improved sensitivity to differences between tasks, and reduced undesirable variability. Muscular rest was more sensitive to task differences than APDF percentiles.
38.	Hansson, Gert-Åke, et al. (author) Technical measurements of physical workload on wrist and forearm in various types of work. 2009 Conference paper (peer-reviewed)
39.	Hedberg, Emma, et al. (author) Chemical and physical characterization of emissions from birch wood combustion in a wood stove 2002 In: Atmospheric Environment. - 1352-2310. ; 36:30, s. 4823-4837 Journal article (peer-reviewed)
40.	Henriksson, Eva, et al. (author) Comparison of cisplatin sensitivity and the 18F fluoro-2-deoxy 2 glucose uptake with proliferation parameters and gene expression in squamous cell carcinoma cell lines of the head and neck 2009 In: Journal of Experimental & Clinical Cancer Research. - : Springer Science and Business Media LLC. - 1756-9966. ; 28 Journal article (peer-reviewed)abstract Background: The survival of patients with locally advanced head and neck cancer is still poor, with 5-year survival rates of 24-35%. The identification of prognostic and predictive markers at the molecular and cellular level could make it possible to find new therapeutic targets and provide "taylor made" treatments. Established cell lines of human squamous cell carcinoma (HNSCC) are valuable models for identifying such markers. The aim of this study was to establish and characterize a series of cell lines and to compare the cisplatin sensitivity and 18F fluoro-2 deoxy 2 glucose (18F-FDG) uptake of these cell lines with other cellular characteristics, such as proliferation parameters and TP53 and CCND1 status. Methods: Explant cultures of fresh tumour tissue were cultivated, and six new permanent cell lines were established from 18 HNSCC cases. Successfully grown cell lines were analysed regarding clinical parameters, histological grade, karyotype, DNA ploidy, and index and S-phase fraction (Spf). The cell lines were further characterized with regard to their uptake of 18F-FDG, their sensitivity to cisplatin, as measured by a viability test ( crystal violet), and their TP53 and CCND1 status, by fluorescence in situ hybridization (FISH), polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) with DNA sequencing and, for cyclin D1, by immunohistochemistry. Results: Patients with tumours that could be cultured in vitro had shorter disease-free periods and overall survival time than those whose tumours did not grow in vitro, when analysed with the Kaplan-Meier method and the log-rank test. Their tumours also showed more complex karyotypes than tumours from which cell lines could not be established. No correlation was found between TP53 or CCND1 status and 18F-FDG uptake or cisplatin sensitivity. However, there was an inverse correlation between tumour cell doubling time and 18F-FDG uptake. Conclusion: In vitro growth of HNSCC cells seem to be an independent prognostic factor, with cell lines being more readily established from aggressive tumours, a phenomenon more dependent on the molecular genetic characteristics of the tumour cells than on tumour location or TNM status.
41.	Isaksson, Olle, 1943, et al. (author) GH and bone--experimental and clinical studies. 2000 In: Endocrine journal. - 0918-8959. ; 47 Suppl Journal article (peer-reviewed)abstract GH increases bone formation both via a direct interaction with GH receptors on osteoblasts and via locally produced IGF-I (autocrine/paracrine action). GH deficiency results in decreased bone mass in both man and laboratory animals and treatment of GHD patients with GH for several months results in increased bone mass. GH treatment also increases bone mass and the total mechanical strength of bones in rats with normal GH secretion. Because of the short duration of GH-treatment in man with normal GH secretion, the effect on bone mass is still inconclusive. The action of GH on bone metabolism in GHD adults is twofold: It stimulates both bone resorption and bone formation. A "Biphasic model" of GH action in bone remodeling has recently been proposed [1] (Fig. 2). According to this model the net effect of GH first results in a loss of bone mass, followed by a net increase in bone mass. The transition point occurs when bone formation proceeds at a higher rate than bone resorption. Taking all clinical studies of GH-treatment of GHD adults into account, it appears that the "transition point" occurs after approximately six months and that a net increase in bone mass usually is seen after 12-18 months of GH treatment. It should be emphasized that the "Biphasic model" of GH action in bone remodeling is proposed based on findings in GHD adults, and it remains to be clarified whether or not it is valid for subjects with normal GH secretion.
42.	Isinger Ekstrand, Anna, et al. (author) Genetic profiles of gastroesophageal cancer: combined analysis using expression array and tiling array-comparative genomic hybridization 2010 In: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608. ; 200:2, s. 120-126 Journal article (peer-reviewed)abstract We aimed to characterize the genomic profiles of adenocarcinomas in the gastroesophageal junction in relation to cancers in the esophagus and the stomach. Profiles of gains/losses as well as gene expression profiles were obtained from 27 gastroesophageal adenocarcinomas by means of 32k high-resolution array-based comparative genomic hybridization and 27k oligo gene expression arrays, and putative target genes were validated in an extended series. Adenocarcinomas in the distal esophagus and the gastroesophageal junction showed strong similarities with the most common gains at 20q13, 8q24, 1q21-23, 5p15, 13q34, and 12q13, whereas different profiles with gains at 5p15, 7p22, 2q35, and 13q34 characterized gastric cancers. CDK6 and EGFR were identified as putative target genes in cancers of the esophagus and the gastroesophageal junction, with upregulation in one quarter of the tumors. Gains/losses and gene expression profiles show strong similarity between cancers in the distal esophagus and the gastroesophageal junction with frequent upregulation of CDK6 and EGFR, whereas gastric cancer displays distinct genetic changes. These data suggest that molecular diagnostics and targeted therapies can be applied to adenocarcinomas of the distal esophagus and gastroesophageal junction alike. (C) 2010 Elsevier Inc. All rights reserved.
43.	Jansson, John-Olov, 1954, et al. (author) Body weight homeostat that regulates fat mass independently of leptin in rats and mice. 2018 In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 115:2, s. 427-432 Journal article (peer-reviewed)abstract Subjects spending much time sitting have increased risk of obesity but the mechanism for the antiobesity effect of standing is unknown. We hypothesized that there is a homeostatic regulation of body weight. We demonstrate that increased loading of rodents, achieved using capsules with different weights implanted in the abdomen or s.c. on the back, reversibly decreases the biological body weight via reduced food intake. Importantly, loading relieves diet-induced obesity and improves glucose tolerance. The identified homeostat for body weight regulates body fat mass independently of fat-derived leptin, revealing two independent negative feedback systems for fat mass regulation. It is known that osteocytes can sense changes in bone strain. In this study, the body weight-reducing effect of increased loading was lost in mice depleted of osteocytes. We propose that increased body weight activates a sensor dependent on osteocytes of the weight-bearing bones. This induces an afferent signal, which reduces body weight. These findings demonstrate a leptin-independent body weight homeostat ("gravitostat") that regulates fat mass.
44.	Kalbin, Georgi, et al. (author) Ultraviolet-B-radiation-induced changes in nicotinamide and glutathione metabolism and gene expression in plants 1997 In: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 249:2, s. 465-472 Journal article (peer-reviewed)abstract Pea (Pisum sativum L. cv. Greenfeast) plants were exposed to supplementary ultraviolet-B (UV-B) radiation (biologically effective dose rates normalised to 300 nm UV-B-BE,B-300: 0.18, 0.32 or 1.4 W m(-2)). Leaf nicotinamide, trigonelline, GSH(tot) (total glutathione) and (GSSG (oxidised glutathione) levels remained unchanged after exposure to the lowest dose rates. 1.4 W m(-2) UV-B-BE,B-300 gave rise to 60-fold and 4.5-fold increases in GSSG and GSH(tot), respectively. 3.5-fold and 9.5-fold increases were found in nicotinamide and trigonelline, respectively. cab (Chlorophyll-a/b-binding protein) transcript levels decreased and CHS (chalcone synthase) and PAL (phenylalanine ammonia-lyase) mRNA increased after shorter UV-B exposures (hours) to the higher dose rate of UV-B and after exposure to the intermediate dose rate. CHS and PAL mRNAs also increased after prolonged exposure to the lowest dose rate. cab transcripts completely disappeared. whereas CHS and PAL mRNA levels rose by 60-fold and 17-fold, respectively, after 12 h exposure at the highest dose rate and 12 h of development. Our results indicate that nicotinamide or trigonelline do not function as signalling compounds for CHS and PAL gene expression. Elevated nicotinamide and trigonelline levels occur in response to UV-B, but only at UV-B doses high enough to cause oxidative stress.
45.	Kalita, Saurav, et al. (author) Energy performance of compressed biomethane gas production from co-digestion of Salix and dairy manure: factoring differences between Salix varieties 2023 In: Biotechnology for Biofuels and Bioproducts. - 2731-3654. ; 16 Journal article (peer-reviewed)abstract Biogas from anaerobic digestion is a versatile energy carrier that can be upgraded to compressed biomethane gas (CBG) as a renewable and sustainable alternative to natural gas. Organic residues and energy crops are predicted to be major sources of bioenergy production in the future. Pre-treatment can reduce the recalcitrance of lignocellulosic energy crops such as Salix to anaerobic digestion, making it a potential biogas feedstock. This lignocellulosic material can be co-digested with animal manure, which has the complementary effect of increasing volumetric biogas yield. Salix varieties exhibit variations in yield, composition and biomethane potential values, which can have a significant effect on the overall biogas production system. This study assessed the impact of Salix varietal differences on the overall mass and energy balance of a co-digestion system using steam pre-treated Salix biomass and dairy manure (DaM) to produce CBG as the final product. Six commercial Salix varieties cultivated under unfertilised and fertilised conditions were compared. Energy and mass flows along this total process chain, comprising Salix cultivation, steam pre-treatment, biogas production and biogas upgrading to CBG, were evaluated. Two scenarios were considered: a base scenario without heat recovery and a scenario with heat recovery. The results showed that Salix variety had a significant effect on energy output-input ratio (R), with R values in the base scenario of 1.57-1.88 and in the heat recovery scenario of 2.36-2.94. In both scenarios, unfertilised var. Tordis was the best energy performer, while the fertilised var. Jorr was the worst. Based on this energy performance, Salix could be a feasible feedstock for co-digestion with DaM, although its R value was at the lower end of the range reported previously for energy crops.
46.	Karlsson, Magnus, et al. (author) Inferior physical performance tests in 10,998 men in the MrOS study is associated with recurrent falls 2012 In: Age and Ageing. - : Oxford University Press (OUP). - 0002-0729 .- 1468-2834. ; 41:6, s. 740-746 Journal article (peer-reviewed)abstract Background: recurrent fallers are at especially high risk for injuries. Objective: to study whether tests of physical performance are associated with recurrent falls. Subjects: a total of 10,998 men aged 65 years or above. Methods: questionnaires evaluated falls sustained 12 months preceding testing of grip strength, timed stand, 6-m walk and 20-cm narrow walk test. Means with 95% confidence interval (95% CI) are reported. P < 0.01 is a statistically significant difference. Results: in comparison to both occasional fallers and non-fallers, recurrent fallers performed more poorly on all the physical ability tests (all P < 0.001). A score below -2 standard deviations (SDs) in the right-hand grip strength test was associated with an odds ratio of 2.4 (95% CI 1.7, 3.4) for having had recurrent falls compared with having had no fall and of 2.0 (95% CI 1.3, 3.4) for having had recurrent falls compared with having had an occasional fall. Conclusion: low performance in physical ability tests are in elderly men associated with recurrent falls.
47.	Karlsson, Magnus, et al. (author) International and ethnic variability of falls in older men 2014 In: Scandinavian journal of public health. - : SAGE Publications. - 1651-1905 .- 1403-4948. ; 42:2, s. 194-200 Journal article (peer-reviewed)abstract Aims: Fallers and especially recurrent fallers are at high risk for injuries. The aim of this study was to evaluate fall epidemiology in older men with special attention to the influence of age, ethnicity and country of residence. Methods: 10,998 men aged 65 years or above recruited in Hong Kong, the United States (US) and Sweden were evaluated in a cross-sectional retrospective study design. Self-reported falls and fractures for the preceding 12 months were registered through questionnaires. Group comparisons were done by chi-square test or logistic regression. Results: The proportion of fallers among the total population was 16.5% in ages 65-69, 24.8% in ages 80-84 and 43.2% in ages above 90 (P <0.001). The corresponding proportions of recurrent fallers in the same age groups were 6.3%, 10.1% and 18.2%, respectively (P <0.001), and fallers with fractures 1.0%, 2.3% and 9.1%, respectively (P <0.001). The proportion of fallers was highest in the US, intermediate in Sweden and lowest in Hong Kong (in most age groups P <0.05). The proportion of fallers among white men in the US was higher than in white men in Sweden (all comparable age groups P <0.01) but there were no differences in the proportion of fallers in US men with different ethnicity. Conclusions: The proportion of fallers in older men is different in different countries, and data in this study corroborate with the view that society of residence influences fall prevalence more than ethnicity.
48.	Karlsson, Magnus K, et al. (author) Characteristics of Prevalent Vertebral Fractures Predict New Fractures in Elderly Men. 2016 In: Journal of Bone and Joint Surgery. American Volume. - 1535-1386. ; 98:5, s. 379-385 Journal article (peer-reviewed)abstract Studies have shown that specific characteristics of prevalent vertebral fractures are associated with a markedly low bone mineral density. This study evaluates if these characteristics also predict subsequent fractures.
49.	Kherad, Mehrsa, et al. (author) Low Clinical Relevance of a Prevalent Vertebral Fracture in Elderly Men - the MrOs Sweden Study 2015 In: The Spine Journal. - : Elsevier BV. - 1878-1632 .- 1529-9430. ; 15:2, s. 281-289 Journal article (peer-reviewed)abstract The epidemiology, the fracture pattern and the clinical relevance of prevalent vertebral fractures in old men are debated wherefore we set out to clarify these issues.
50.	Kherad, Mehrsa, et al. (author) Low clinical relevance of a prevalent vertebral fracture in old men-the MrOs Sweden study. 2015 In: The spine journal : official journal of the North American Spine Society. - : Elsevier BV. - 1878-1632. ; 15:2, s. 281-289 Journal article (peer-reviewed)abstract The epidemiology, the fracture pattern, and the clinical relevance of prevalent vertebral fractures in old men are debated wherefore we set out to clarify these issues.

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