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Träfflista för sökning "WFRF:(Olivo Marin Jean Christophe) "

Search: WFRF:(Olivo Marin Jean Christophe)

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1.
  • Chenouard, Nicolas, et al. (author)
  • Objective comparison of particle tracking methods
  • 2014
  • In: Nature Methods. - : Springer Science and Business Media LLC. - 1548-7091 .- 1548-7105. ; 11:3, s. 281-U247
  • Journal article (peer-reviewed)abstract
    • Particle tracking is of key importance for quantitative analysis of intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting and following large numbers of individual particles is not feasible, automated computational methods have been developed for these tasks by many groups. Aiming to perform an objective comparison of methods, we gathered the community and organized an open competition in which participating teams applied their own methods independently to a commonly defined data set including diverse scenarios. Performance was assessed using commonly defined measures. Although no single method performed best across all scenarios, the results revealed clear differences between the various approaches, leading to notable practical conclusions for users and developers.
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3.
  • Viklund, Ing-Marie, et al. (author)
  • WAFL, a new protein involved in regulation of early endocytictransport at the intersection of actin and microtubule dynamics
  • 2009
  • In: Experimental Cell Research. - : Elsevier Inc.. - 0014-4827 .- 1090-2422. ; 315:6, s. 1040-1052
  • Journal article (peer-reviewed)abstract
    • We have previously identified a new gene with sequence homology to the WASP-family of actin regulators denoted WAFL (WASP and FKBP-like). Here we report a possible biological function for WAFL, by demonstrating an association to early endosomes via its central coiled-coil domain. Further we show by functional and structural studies that WAFL is associated with both microtubules and the actin filament system, the two means of transport of early endosomes. In addition, WAFL interacts with WASP-interacting protein (WIP) and actin, thus linking WAFL to actin dynamics. The use of RNAi depletion of WAFL shows that WAFL-deficient cells display delayed transport of endosomal cargo. Our findings are compatible with a model wherebyWAFL is involved in the transport of early endosomes at the level of transition between microfilamentbased and microtubule-based movement.
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