| 1. |
- Benlliure, J., et al.
(author)
-
New experimental approaches to investigate the fission dynamics
- 2016
-
In: AIP Conference Proceedings. - : Author(s). - 1551-7616 .- 0094-243X. - 9780735414112 ; 1753
-
Conference paper (peer-reviewed)abstract
- The first ever achieved full identification of both fission fragments, in atomic and mass number, made it possible to define new observables sensitive to the fission dynamics along the fission path up to the scission point. Moreover, proton-induced fission of 208Pb at high energies offers optimal conditions for the investigation of dissipative, and transient effects, because of the high-excitation energy of the fissioning nuclei, its low angular momentum, and limited shape distortion by the reaction. In this work we show that the charge distribution of the final fission fragments can constrain the ground-to-saddle dynamics while the mass distribution is sensitive to the dynamics until the scission point.
|
|
| 2. |
|
|
| 3. |
- Cortina-Gil, D., et al.
(author)
-
CALIFA, a Dedicated Calorimeter for the R3B/FAIR
- 2014
-
In: Nuclear Data Sheets. - : Elsevier BV. - 1095-9904 .- 0090-3752. ; 120, s. 99-101
-
Journal article (peer-reviewed)abstract
- The R3B experiment (Reactions with Relativistic Radioactive Beams) at FAIR (Facility for Antiproton and Ion Research) is a versatile setup dedicated to the study of reactions induced by high-energy radioactive beams. It will provide kinematically complete measurements with high efficiency, acceptance and resolution, making possible a broad physics program with rare-isotopes. CALIFA (CALorimeter for In-Flight detection of gamma-rays and high energy charged pArticles), is a complex detector based on scintillation crystals, that will surround the target of the R3B experiment. CALIFA will act as a total absorption gamma-calorimeter and spectrometer, as well as identifier of charged particles from target residues. This versatility is its most challenging requirement, demanding a huge dynamic range, to cover from low energy gamma-rays up to 300 MeV protons. This fact, along with the high-energy of the beams determine the conceptual design of the detector, presented in this paper, together with the technical solutions proposed for its construction.
|
|
| 4. |
- Cortina-Gil, D., et al.
(author)
-
CALIFA, a Dedicated Calorimeter for the (RB)-B-3/FAIR
- 2014
-
In: Nuclear Data Sheets. - : Elsevier BV. - 0090-3752. ; 120, s. 99-101
-
Journal article (peer-reviewed)abstract
- The (RB)-B-3 experiment (Reactions with Relativistic Radioactive Beams) at FAIR (Facility for Antiproton and Ion Research) is a versatile setup dedicated to the study of reactions induced by high-energy radioactive beams. It will provide kinematically complete measurements with high efficiency, acceptance and resolution, making possible a broad physics program with rare-isotopes. CALIFA (CALorimeter for In-Flight detection of gamma-rays and high energy charged pArticles), is a complex detector based on scintillation crystals, that will surround the target of the (RB)-B-3 experiment. CALIFA will act as a total absorption gamma-calorimeter and spectrometer, as well as identifier of charged particles from target residues. This versatility is its most challenging requirement, demanding a huge dynamic range, to cover from low energy gamma-rays up to 300 MeV protons. This fact, along with the high-energy of the beams determine the conceptual design of the detector, presented in this paper, together with the technical solutions proposed for its construction.
|
|
| 5. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Complete characterization of the fission fragments produced in reactions induced by Pb 208 projectiles on proton at 500A MeV
- 2015
-
In: Physical Review C - Nuclear Physics. - 2469-9985 .- 2469-9993. ; 91:6
-
Journal article (peer-reviewed)abstract
- The two fragments produced in fission reactions induced by Pb208 projectiles impinging on a liquid hydrogen target at 500AMeV have been fully identified in coincidence. The experiment was performed at GSI Darmstadt, where the combined use of the inverse kinematics technique with an efficient detection setup permitted one to detect and to fully identify the fission fragments in a range from Z=27 to Z=52. The corresponding isotopic cross sections and velocities of the fission fragments were measured with high accuracy. The results are compared to state-of-the-art model calculations that reproduce the measured observables. Correlations between the two fragments were used to assess the role of charge polarization and the excitation energy gained by the nascent fragments using the neutron excess of the final fragments. The analysis of the average velocities of the fission fragments allowed us to parametrize the distance between the two fission fragments at scission as a function of the size of the fissioning system.
|
|
| 6. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Constraining the level density using fission of lead projectiles
- 2015
-
In: Physical Review C - Nuclear Physics. - 2469-9985 .- 2469-9993. ; 92:4
-
Journal article (peer-reviewed)abstract
- The nuclear level density is one of the main ingredients for the statistical description of the fission process. In this work, we propose to constrain the description of this parameter by using fission reactions induced by protons and light ions on Pb208 at high kinetic energies. The experiment was performed at GSI (Darmstadt), where the combined use of the inverse kinematics technique with an efficient detection setup allowed us to measure the atomic number of the two fission fragments in coincidence. This measurement permitted us to obtain with high precision the partial fission cross sections and the width of the charge distribution as a function of the atomic number of the fissioning system. These data and others previously measured, covering a large range in fissility, are compared to state-of-the-art calculations. The results reveal that total and partial fission cross sections cannot unambiguously constrain the level density at ground-state and saddle-point deformations and additional observables, such as the width of the charge distribution of the final fission fragments, are required.
|
|
| 7. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Dissipative effects in fission investigated in complete kinematics measurements
- 2017
-
In: EPJ Web of Conferences. - : EDP Sciences. - 2101-6275 .- 2100-014X. - 9782759890200 ; 146
-
Conference paper (peer-reviewed)abstract
- The study of dissipative effects in fission has been carried out with fusion-fission reactions by using a limited number of observables, such as the fission probabilities, the mass distribution of the fission fragments, or the neutron multiplicities. However, the large angular momenta gained by the compound nucleus in this kind of reaction could affect the conclusions drawn from such experiments. In this work, we propose to investigate the fission dynamics by the use of spallation reactions on 208Pb because the fissioning systems are produced with low angular momentum, small deformations, and high excitation energies, enhancing the dissipative effects. The complete kinematics measurements of the fission fragments and light-charged particles were performed by the use of the SOFIA setup combined with the inverse kinematics technique, allowing us for the first time a full indentification in atomic and mass number of the two fission fragments. These measurements permit us to define new fission observables for the investigation of the temperature and deformation dependencies of the dissipation parameter.
|
|
| 8. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Dissipative effects in fission investigated with proton-on-lead reactions
- 2016
-
In: EPJ Web of Conferences. - : EDP Sciences. - 2101-6275 .- 2100-014X. - 9788874381012 ; 117, s. Art. no. 08017-
-
Conference paper (peer-reviewed)abstract
- The complete kinematic measurement of the two fission fragments permitted us to investigate dissipative effects at large deformations, between the saddle-point and the corresponding scission configurations. Up to now, this kind of study has only been performed with fusionfission reactions using a limited number of observables, such as the mass distribution of the fission fragments or the neutron multiplicities. However, the large angular momenta gained by the compound nucleus could affect the conclusions drawn from such experiments. In this work, the use of spallation reactions, where the fissioning systems are produced with low angular momentum, small deformations and high excitation energies, favors the study of dissipation, and allowed us to define new observables, such as postscission neutron multiplicities and the neutron excess of the final fission fragments as a function of the atomic number of the fissioning system. These new observables are used to investigate the dissipation at large deformations.
|
|
| 9. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Fission of highly excited nuclei investigated in complete kinematic measurements
- 2013
-
In: EPJ Web of Conferences. - : EDP Sciences. - 2101-6275 .- 2100-014X. ; 62
-
Conference paper (peer-reviewed)abstract
- Fission is an extremely complex mechanism that requires a dynamical approach to describe the evolution of the process in terms of intrinsic and collective excitations of the nuclear constituents. In order to determine these effects a complex experimental setup was mounted at GSI, which allowed us for the first time the full identification in charge and mass of all fission fragments thanks to a magnetic separation and the use of the inverse kinematic technique. Moreover, we also measured the neutron multiplicities and the light-charged particles emitted in coincidence with fission. These complete kinematic measurements will be used to define sensitive observables to dissipative and transient effects in fission. In this manuscript we present the first results for the total fission cross sections. © Owned by the authors, published by EDP Sciences, 2013.
|
|
| 10. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Light charged particles emitted in fission reactions induced by protons on Pb-208
- 2016
-
In: Physical Review C. - 2469-9985 .- 2469-9993. ; 94:3
-
Journal article (peer-reviewed)abstract
- Light charged particles emitted in proton-induced fission reactions on Pb-208 have been measured at different kinetic energies: 370A, 500A, and 650A MeV. The experiment was performed by the SOFIA Collaboration at the GSI facilities in Darmstadt (Germany). The inverse kinematics technique was combined with a setup especially designed to measure light charged particles in coincidence with fission fragments. This measurement allowed us, for the first time, to obtain correlations between the light charged particles emitted during the fission process and the charge distributions of the fission fragments. These correlations were compared with different model calculations to assess the ground-to-saddle dynamics. The results confirm that transient and dissipative effects are required for an accurate description of the fission observables.
|
|
| 11. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Presaddle and postsaddle dissipative effects in fission using complete kinematics measurements
- 2016
-
In: Physical Review C. - 2469-9985 .- 2469-9993. ; 94:6
-
Journal article (peer-reviewed)abstract
- A complete kinematics measurement of the two fission fragments was used for the first time to investigate fission dynamics at small and large deformations. Fissioning systems with high excitation energies, compact shapes, and low angular momenta were produced in inverse kinematics by using spallation reactions of lead projectiles. A new generation experimental setup allowed for the first full and unambiguous identification in mass and atomic number of both fission fragments. This measurement permitted us to accurately determine fission cross sections, the charge distribution, and the neutron excess of the fission fragments as a function of the atomic number of the fissioning system. These data are compared with different model calculations to extract information on the value of the dissipation parameter at small and large deformations. The present results do not show any sizable dependence of the nuclear dissipation parameter on temperature or deformation.
|
|
| 12. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Proton-induced fission cross sections on Pb-208 at high kinetic energies
- 2014
-
In: Physical Review C - Nuclear Physics. - 2469-9985 .- 2469-9993. ; 90:6, s. Art. no. 064606-
-
Journal article (peer-reviewed)abstract
- Total fission cross sections of Pb-208 induced by protons have been determined at 370A, 500A, and 650A MeV. The experiment was performed at GSI Darmstadt where the combined use of the inverse kinematics technique with an efficient detection setup allowed us to determine these cross sections with an uncertainty below 6%. This result was achieved by an accurate beam selection and registration of both fission fragments in coincidence which were also clearly distinguished from other reaction channels. These data solve existing discrepancies between previous measurements, providing new values for the Prokofiev systematics. The data also allow us to investigate the fission process at high excitation energies and small deformations. In particular, some fundamental questions about fission dynamics have been addressed, which are related to dissipative and transient time effects.
|
|
| 13. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Transient effects in fission investigated with proton-on-lead reactions in complete kinematic measurements
- 2015
-
In: Physics Procedia. - : Elsevier BV. - 1875-3884 .- 1875-3892. ; 64, s. 157-162
-
Conference paper (peer-reviewed)abstract
- Proton-induced fission of Pb-208 has been investigated at 370, 500 and 650 A MeV in complete kinematic measurements. The experiment was performed at GSI Darmstadt where the combined use of the inverse kinematics technique with an efficient detection setup allowed to measure for the first time the atomic and mass number of both fission fragments. The performed measurement together with different model calculations allow us to investigate dissipative and transient effects in the fission process and the dependence on temperature and deformation of the dissipation strength beta. The use of spallation reactions with lead projectiles favours this study due to its high excitation energy and low angular momentum. (C) 2015 The Authors. Published by Elsevier B.V.
|
|
| 14. |
- Cabanelas, P., et al.
(author)
-
Performance recovery of long CsI(Tl) scintillator crystals with APD-based readout
- 2020
-
In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. - : Elsevier BV. - 0168-9002. ; 965
-
Journal article (peer-reviewed)abstract
- CALIFA is the high efficiency and energy resolution calorimeter for the R3B experiment at FAIR, intended for detecting high energy light charged particles and gamma rays in scattering experiments, and is being commissioned during the Phase-0 experiments at FAIR, between 2018 and 2020. It surrounds the reaction target in a segmented configuration with 2432 detection units made of long CsI(Tl) finger-shaped scintillator crystals. CALIFA has a 10 year intended operational lifetime as the R3B calorimeter, necessitating measures to be taken to ensure enduring performance. In this paper we present a systematic study of two groups of 6 different detection units of the CALIFA detector after more than four years of operation. The energy resolution and light output yield are evaluated under different conditions. Tests cover the aging of the first detector units assembled and investigates recovery procedures for degraded detection units. A possible reason for the observed degradation is given, pointing to the crystal-APD coupling.
|
|
| 15. |
- Diaz Fernandez, Paloma, 1983, et al.
(author)
-
Quasifree (p, pN) scattering of light neutron-rich nuclei near N=14
- 2018
-
In: Physical Review C. - 2469-9985 .- 2469-9993. ; 97:2
-
Journal article (peer-reviewed)abstract
- Background: For many years, quasifree scattering reactions in direct kinematics have been extensively used to study the structure of stable nuclei, demonstrating the potential of this approach. The (RB)-B-3 collaboration has performed a pilot experiment to study quasifree scattering reactions in inverse kinematics for a stable C-12 beam. The results from that experiment constitute the first quasifree scattering results in inverse and complete kinematics. This technique has lately been extended to exotic beams to investigate the evolution of shell structure, which has attracted much interest due to changes in shell structure if the number of protons or neutrons is varied. Purpose: In this work we investigate for the first time the quasifree scattering reactions (p, pn) and (p, 2p) simultaneously for the same projectile in inverse and complete kinematics for radioactive beams with the aim to study the evolution of single-particle properties from N = 14 to N = 15. Method: The structure of the projectiles O-23, O-22, and N-21 has been studied simultaneously via (p, pn) and (p, 2p) quasifree knockout reactions in complete inverse kinematics, allowing the investigation of proton and neutron structure at the same time. The experimental data were collected at the (RB)-B-3-LAND setup at GSI at beam energies of around 400 MeV/u. Two key observables have been studied to shed light on the structure of those nuclei: the inclusive cross sections and the corresponding momentum distributions. Conclusions: The knockout reactions (p, pn) and (p, 2p) with radioactive beams in inverse kinematics have provided important and complementary information for the study of shell evolution and structure. For the (p, pn) channels, indications of a change in the structure of these nuclei moving from N = 14 to N = 15 have been observed, i.e., from the 0d(5/2) shell to the 1s(1/2). This supports previous observations of a subshell closure at N = 14 for neutron-rich oxygen isotopes and its weakening for the nitrogen isotopes.
|
|
| 16. |
- Haller, B K, et al.
(author)
-
Therapeutic efficacy of a DNA vaccine targeting the endothelial tip cell antigen delta-like ligand 4 in mammary carcinoma.
- 2010
-
In: Oncogene. - : Springer Science and Business Media LLC. - 1476-5594 .- 0950-9232. ; 29:30, s. 4276-86
-
Journal article (peer-reviewed)abstract
- The Notch ligand delta-like ligand 4 (DLL4) is an essential component expressed by endothelial tip cells during angiogenic sprouting. We have described a conceptually novel therapeutic strategy for targeting tumor angiogenesis and endothelial tip cells based on DNA vaccination against DLL4. Immunization with DLL4-encoding plasmid DNA by in vivo electroporation severely retarded the growth of orthotopically implanted mammary carcinomas in mice by induction of a nonproductive angiogenic response. Mechanistically, vaccination brought about a break in tolerance against the self-antigen, DLL4, as evidenced by the production of inhibitory and inherently therapeutic antibodies against mouse DLL4. Importantly, no evidence for a delayed wound healing response, or for toxicity associated with pharmacological blockade of DLL4 signaling, was noted in mice immunized with the DLL4 vaccine. We have thus developed a well-tolerated DNA vaccination strategy targeting the endothelial tip cells and the antigen DLL4 with proven therapeutic efficacy in mouse models of mammary carcinoma; a disease that has been reported to dramatically induce the expression of DLL4. Conceivably, induction of immunity toward principal mediators of pathological angiogenesis could provide protection against recurrent malignant disease in the adjuvant setting.
|
|
| 17. |
- Nacher, E., et al.
(author)
-
Proton response of CEPA4: A novel LaBr3(Ce)-LaCl3(Ce) phoswich array for high-energy gamma and proton spectroscopy
- 2015
-
In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. - : Elsevier BV. - 0168-9002. ; 769, s. 105-111
-
Journal article (peer-reviewed)abstract
- A new phoswich array, for the detection of high-energy protons and gamma rays from nuclear reactions, has been built. This new detector consists of four individual closely packed scintillator detectors, each of them made of 4 cm of LaBr3(Ce) and G cm of LaCl3(Ce) in phoswich configuration (optically coupled and with a common readout). In this paper we report on the results of a beam test performed at the Bronowice Cyclotron Centre (CCB) in Krakow, showing the response of this versatile instrument to high energy protons (70-230 MeV). Furthermore, for the first time we prove that we can reconstruct the original energy of fast protons (E > 200 MeV) which pass through the total length of the crystal while still retaining a good energy resolution.
|
|
| 18. |
- Rodriguez-Sanchez, J. L., et al.
(author)
-
Fission dynamics at high excitation energies investigated in complete kinematics measurements
- 2017
-
In: Journal of Physics: Conference Series. - : IOP Publishing. - 1742-6588 .- 1742-6596. ; 863:1, s. Article no 012047-
-
Conference paper (peer-reviewed)abstract
- Light-charged particles emitted in proton-induced fission reactions on 208 Pb have been measured at different kinetic energies: 370A, 500A, and 650A MeV. The experiment was performed by the SOFIA collaboration at the GSI facilities in Darmstadt (Germany). The inverse kinematics technique was combined with a setup especially designed to measure light-charged particles in coincidence with fission fragments. The data were compared with different model calculations to assess the ground-to-saddle dynamics. The results confirm that transient and dissipative effects are required for an accurate description of the fission observables.
|
|
| 19. |
- Alvarez-Pol, H., et al.
(author)
-
Performance analysis for the CALIFA Barrel calorimeter of the (RB)-B-3 experiment
- 2014
-
In: Nuclear Instruments & Methods in Physics Research. Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment. - : Elsevier BV. - 0167-5087 .- 0168-9002. ; 767, s. 453-466
-
Journal article (peer-reviewed)abstract
- The CALIFA calorimeter is an advanced detector for gamma rays and light charged particles, accordingly optimized for the demanding requirements of the physics programme proposed for the (RB)-B-3 facility at FAIR. The multipurpose character of CALIFA is required to fulfil challenging demands in energy resolution (5-6% at 1 MeV for gamma rays) and efficiency. Charged particles, e.g. protons of energies up to 320 MeV in the Barrel section, should also be identified with an energy resolution better to 1%. CALIFA is divided into two well-separated sections: a "Forward EndCap" and a cylindrical "Barrel" covering an angular range from 43.2 degrees to 140.3 degrees. The Barrel section, based on long CsI(Tl) pyramidal frustum crystals coupled to large area avalanche photodiodes (LAAPDs), attains the requested high efficiency for calorimetric purposes. The construction of the CALIFA Demonstrator, comprising 20% of the total detector, has already been initiated, and commissioning experiments are expected for 2014. The assessment of the capabilities and expected performance of the detector elements is a crucial step in their design, along with the prototypes evaluation. For this purpose, the Barrel geometry has been carefully implemented in the simulation package R3BRoot, including easily variable thicknesses of crystal wrapping and carbon fibre supports. A complete characterization of the calorimeter response (including efficiency, resolution, evaluation of energy and reconstruction losses) under different working conditions, with several physics cases selected to probe the detector performance over a wide range of applications, has been undertaken. Prototypes of different sections of the CALIFA Barrel have been modeled and their responses have been evaluated and compared with the experimental results. The present paper summarizes the outcome of the simulation campaign for the entire Barrel section and for the corresponding prototypes tested at different European installations. (C) 2014 Elsevier B.V. All rights reserved
|
|
| 20. |
|
|
| 21. |
- Cunha, Sara I., et al.
(author)
-
Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer.
- 2015
-
In: Cancer Research. - 1538-7445 .- 0008-5472. ; 75:12, s. 2445-2456
-
Journal article (peer-reviewed)abstract
- Exploration of new strategies for the prevention of breast cancer metastasis is justifiably at the center of clinical attention. In this study, we combined a computational biology approach with mechanism-based preclinical trials to identify inhibitors of activin-like receptor kinase (ALK) 1 as effective agents for blocking angiogenesis and metastasis in breast cancer. Pharmacologic targeting of ALK1 provided long-term therapeutic benefit in mouse models of mammary carcinoma, accompanied by strikingly reduced metastatic colonization as a monotherapy or part of combinations with chemotherapy. Gene-expression analysis of breast cancer specimens from a population-based nested case-control study encompassing 768 subjects defined endothelial expression of ALK1 as an independent and highly specific prognostic factor for metastatic manifestation, a finding that was corroborated in an independent clinical cohort. Overall, our results suggest that pharmacologic inhibition of endothelial ALK1 constitutes a tractable strategy for interfering with metastatic dissemination of breast cancer. Cancer Res; 75(12); 2445-56. ©2015 AACR.
|
|
| 22. |
- Dass, Randall A., et al.
(author)
-
Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by RNA Polymerase I
- 2016
-
In: PLoS Genetics. - : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 12:8
-
Journal article (peer-reviewed)abstract
- Ribosome biogenesis is essential for cell growth and proliferation and is commonly elevated in cancer. Accordingly, numerous oncogene and tumor suppressor signaling pathways target rRNA synthesis. In breast cancer, non-canonical Wnt signaling by Wnt5a has been reported to antagonize tumor growth. Here, we show that Wnt5a rapidly represses rDNA gene transcription in breast cancer cells and generates a chromatin state with reduced transcription of rDNA by RNA polymerase I (Pol I). These effects were specifically dependent on Dishevelled1 (DVL1), which accumulates in nucleolar organizer regions (NORs) and binds to rDNA regions of the chromosome. Upon DVL1 binding, the Pol I transcription activator and deacetylase Sirtuin 7 (SIRT7) releases from rDNA loci, concomitant with disassembly of Pol I transcription machinery at the rDNA promoter. These findings reveal that Wnt5a signals through DVL1 to suppress rRNA transcription. This provides a novel mechanism for how Wnt5a exerts tumor suppressive effects and why disruption of Wnt5a signaling enhances mammary tumor growth in vivo.
|
|
| 23. |
|
|
| 24. |
|
|
| 25. |
- Englund, E, et al.
(author)
-
Cartilage oligomeric matrix protein contributes to the development and metastasis of breast cancer
- 2016
-
In: Oncogene. - : Springer Science and Business Media LLC. - 1476-5594 .- 0950-9232. ; 35:43, s. 5585-5596
-
Journal article (peer-reviewed)abstract
- Cartilage oligomeric matrix protein (COMP) is a soluble pentameric protein expressed in cartilage and involved in collagen organization. Tissue microarrays derived from two cohorts of patients with breast cancer (n=122 and n=498) were immunostained, revealing varying expression of COMP, both in the tumor cells and surrounding stroma. High levels of COMP in tumor cells correlated, independently of other variables, with poor survival and decreased recurrence-free survival. Breast cancer cells, MDA-MB-231, stably expressing COMP were injected into the mammary fat pad of SCID (CB-17/Icr-Prkdc(scid)/Rj) mice. Tumors expressing COMP were significantly larger and were more prone to metastasize as compared with control, mock-transfected, tumors. In vitro experiments confirmed that COMP-expressing cells had a more invasive phenotype, which could in part be attributed to an upregulation of matrix metalloprotease-9. Furthermore, microarray analyses of gene expression in tumors formed in vivo showed that COMP expression induced higher expression of genes protecting against endoplasmic reticulum stress. This observation was confirmed in vitro as COMP-expressing cells showed better survival as well as a higher rate of protein synthesis when treated with brefeldin A, compared with control cells. Further, COMP-expressing cells appeared to undergo a metabolic switch, that is, a Warburg effect. Thus, in vitro measurement of cell respiration indicated decreased mitochondrial metabolism. In conclusion, COMP is a novel biomarker in breast cancer, which contributes to the severity of the disease by metabolic switching and increasing invasiveness and tumor cell viability, leading to reduced survival in animal models and human patients.
|
|
| 26. |
|
|
| 27. |
|
|
| 28. |
|
|
| 29. |
|
|
| 30. |
|
|
| 31. |
- Nowak-Sliwinska, Patrycja, et al.
(author)
-
Consensus guidelines for the use and interpretation of angiogenesis assays
- 2018
-
In: Angiogenesis. - : Springer. - 0969-6970 .- 1573-7209. ; 21:3, s. 425-532
-
Research review (peer-reviewed)abstract
- The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
|
|
| 32. |
|
|
| 33. |
|
|
| 34. |
- Pietras, B., et al.
(author)
-
First testing of the CALIFA Barrel Demonstrator
- 2016
-
In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. - : Elsevier BV. - 0168-9002. ; 814, s. 56-65
-
Journal article (peer-reviewed)abstract
- Advancement of the CALIFA calorimeter project has reached a new milestone with the construction of the first modules of the CALIFA Demonstrator, ultimately to be integrated into the final calorimeter. Aspects and methods of detector optimisation will be discussed, along with characterisation using proton beams of 70
|
|
| 35. |
|
|
| 36. |
- Prakash, Varsha, et al.
(author)
-
Ribosome biogenesis during cell cycle arrest fuels EMT in development and disease
- 2019
-
In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10
-
Journal article (peer-reviewed)abstract
- Ribosome biogenesis is a canonical hallmark of cell growth and proliferation. Here we show that execution of Epithelial-to-Mesenchymal Transition (EMT), a migratory cellular program associated with development and tumor metastasis, is fueled by upregulation of ribosome biogenesis during G1/S arrest. This unexpected EMT feature is independent of species and initiating signal, and is accompanied by release of the repressive nucleolar chromatin remodeling complex (NoRC) from rDNA, together with recruitment of the EMT-driving transcription factor Snai1 (Snail1), RNA Polymerase I (Pol I) and the Upstream Binding Factor (UBF). EMT-associated ribosome biogenesis is also coincident with increased nucleolar recruitment of Rictor, an essential component of the EMT-promoting mammalian target of rapamycin complex 2 (mTORC2). Inhibition of rRNA synthesis in vivo differentiates primary tumors to a benign, Estrogen Receptor-alpha (ER alpha) positive, Rictor-negative phenotype and reduces metastasis. These findings implicate the EMT-associated ribosome biogenesis program with cellular plasticity, de-differentiation, cancer progression and metastatic disease.
|
|
| 37. |
|
|
