| 1. |
- Klionsky, Daniel J., et al.
(author)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
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| 3. |
- Richards, Stephen, et al.
(author)
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Genome Sequence of the Pea Aphid Acyrthosiphon pisum
- 2010
-
In: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 8:2, s. e1000313-
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Journal article (peer-reviewed)abstract
- Aphids are important agricultural pests and also biological models for studies of insect-plant interactions, symbiosis, virus vectoring, and the developmental causes of extreme phenotypic plasticity. Here we present the 464 Mb draft genome assembly of the pea aphid Acyrthosiphon pisum. This first published whole genome sequence of a basal hemimetabolous insect provides an outgroup to the multiple published genomes of holometabolous insects. Pea aphids are host-plant specialists, they can reproduce both sexually and asexually, and they have coevolved with an obligate bacterial symbiont. Here we highlight findings from whole genome analysis that may be related to these unusual biological features. These findings include discovery of extensive gene duplication in more than 2000 gene families as well as loss of evolutionarily conserved genes. Gene family expansions relative to other published genomes include genes involved in chromatin modification, miRNA synthesis, and sugar transport. Gene losses include genes central to the IMD immune pathway, selenoprotein utilization, purine salvage, and the entire urea cycle. The pea aphid genome reveals that only a limited number of genes have been acquired from bacteria; thus the reduced gene count of Buchnera does not reflect gene transfer to the host genome. The inventory of metabolic genes in the pea aphid genome suggests that there is extensive metabolite exchange between the aphid and Buchnera, including sharing of amino acid biosynthesis between the aphid and Buchnera. The pea aphid genome provides a foundation for post-genomic studies of fundamental biological questions and applied agricultural problems.
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| 4. |
- Elsik, Christine G., et al.
(author)
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The Genome Sequence of Taurine Cattle : A Window to Ruminant Biology and Evolution
- 2009
-
In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 324:5926, s. 522-528
-
Journal article (peer-reviewed)abstract
- To understand the biology and evolution of ruminants, the cattle genome was sequenced to about sevenfold coverage. The cattle genome contains a minimum of 22,000 genes, with a core set of 14,345 orthologs shared among seven mammalian species of which 1217 are absent or undetected in noneutherian (marsupial or monotreme) genomes. Cattle-specific evolutionary breakpoint regions in chromosomes have a higher density of segmental duplications, enrichment of repetitive elements, and species-specific variations in genes associated with lactation and immune responsiveness. Genes involved in metabolism are generally highly conserved, although five metabolic genes are deleted or extensively diverged from their human orthologs. The cattle genome sequence thus provides a resource for understanding mammalian evolution and accelerating livestock genetic improvement for milk and meat production.
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| 5. |
- Sodergren, Erica, et al.
(author)
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The genome of the sea urchin Strongylocentrotus purpuratus.
- 2006
-
In: Science. - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 314:5801, s. 941-52
-
Journal article (peer-reviewed)abstract
- We report the sequence and analysis of the 814-megabase genome of the sea urchin Strongylocentrotus purpuratus, a model for developmental and systems biology. The sequencing strategy combined whole-genome shotgun and bacterial artificial chromosome (BAC) sequences. This use of BAC clones, aided by a pooling strategy, overcame difficulties associated with high heterozygosity of the genome. The genome encodes about 23,300 genes, including many previously thought to be vertebrate innovations or known only outside the deuterostomes. This echinoderm genome provides an evolutionary outgroup for the chordates and yields insights into the evolution of deuterostomes.
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| 6. |
- Werren, John H, et al.
(author)
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Functional and evolutionary insights from the genomes of three parasitoid Nasonia species.
- 2010
-
In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 327:5963, s. 343-8
-
Journal article (peer-reviewed)abstract
- We report here genome sequences and comparative analyses of three closely related parasitoid wasps: Nasonia vitripennis, N. giraulti, and N. longicornis. Parasitoids are important regulators of arthropod populations, including major agricultural pests and disease vectors, and Nasonia is an emerging genetic model, particularly for evolutionary and developmental genetics. Key findings include the identification of a functional DNA methylation tool kit; hymenopteran-specific genes including diverse venoms; lateral gene transfers among Pox viruses, Wolbachia, and Nasonia; and the rapid evolution of genes involved in nuclear-mitochondrial interactions that are implicated in speciation. Newly developed genome resources advance Nasonia for genetic research, accelerate mapping and cloning of quantitative trait loci, and will ultimately provide tools and knowledge for further increasing the utility of parasitoids as pest insect-control agents.
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| 7. |
- Zhao, Chaoyang, et al.
(author)
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A massive expansion of effector genes underlies gall-formation in the wheat pest Mayetiola destructor
- 2015
-
In: Current Biology. - : Elsevier BV. - 1879-0445 .- 0960-9822. ; 25:5, s. 613-620
-
Journal article (peer-reviewed)abstract
- Gall-forming arthropods are highly specialized herbivores that, in combination with their hosts, produce extended phenotypes with unique morphologies [1]. Many are economically important, and others have improved our understanding of ecology and adaptive radiation [2]. However, the mechanisms that these arthropods use to induce plant galls are poorly understood. We sequenced the genome of the Hessian fly (Mayetiola destructor; Diptera: Cecidomyiidae), a plant parasitic gall midge and a pest of wheat (Triticum spp.), with the aim of identifying genic modifications that contribute to its plant-parasitic lifestyle. Among several adaptive modifications, we discovered an expansive reservoir of potential effector proteins. Nearly 5% of the 20,163 predicted gene models matched putative effector gene transcripts present in the M. destructor larval salivary gland. Another 466 putative effectors were discovered among the genes that have no sequence similarities in other organisms. The largest known arthropod gene family (family SSGP-71) was also discovered within the effector reservoir. SSGP-71 proteins lack sequence homologies to other proteins, but their structures resemble both ubiquitin E3 ligases in plants and E3-ligase-mimicking effectors in plant pathogenic bacteria. SSGP-71 proteins and wheat Skp proteins interact in vivo. Mutations in different SSGP-71 genes avoid the effector-triggered immunity that is directed by the wheat resistance genes H6 and H9. Results point to effectors as the agents responsible for arthropod-induced plant gall formation.
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| 8. |
- Ablikim, M., et al.
(author)
-
Amplitude analysis of the pi(0)pi(0) system produced in radiative J/psi decays
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:5
-
Journal article (peer-reviewed)abstract
- An amplitude analysis of the pi(0)pi(0) system produced in radiative J/psi decays is presented. In particular, a piecewise function that describes the dynamics of the pi(0)pi(0) system is determined as a function of M pi(0)pi(0) from an analysis of the (1.311 +/- 0.011) x 10(9) J/psi decays collected by the BESIII detector. The goal of this analysis is to provide a description of the scalar and tensor components of the pi(0)pi(0) system while making minimal assumptions about the properties or number of poles in the amplitude. Such a model-independent description allows one to integrate these results with other related results from complementary reactions in the development of phenomenological models, which can then be used to directly fit experimental data to obtain parameters of interest. The branching fraction of J/psi -> pi(0)pi(0) is determined to be (1.15 +/- 0.05) x 10(-3), where the uncertainty is systematic only and the statistical uncertainty is negligible.
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| 9. |
- Ablikim, M., et al.
(author)
-
An improved limit for Gamma(ee) of X(3872) and Gamma(ee) measurement of psi(3686)
- 2015
-
In: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 749, s. 414-420
-
Journal article (peer-reviewed)abstract
- Using the data sets taken at center-of-mass energies above 4 GeV by the BESIII detector at the BEPCII storage ring, we search for the reaction e(+)e(-) -> gamma(ISR) X(3872) -> gamma(ISR)pi(+)pi(-) J/psi via the Initial State Radiation technique. The production of a resonance with quantum numbers J(PC) = 1(++) such as the X(3872) via single photon e(+)e(-) annihilation is forbidden, but is allowed by a next-to-leading order box diagram. We do not observe a significant signal of X(3872), and therefore give an upper limit for the electronic width times the branching fraction Gamma B-X(3872)(ee)(X(3872) -> pi(+)pi(-) J/psi) < 0.13 eVat the 90% confidence level. This measurement improves upon existing limits by a factor of 46. Using the same final state, we also measure the electronic width of the psi(3686) to be Gamma(psi)(ee)(3686) ee = 2213 +/- 18(stat) +/- 99(sys) eV.
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| 10. |
- Ablikim, M., et al.
(author)
-
Dark photon search in the mass range between 1.5 and 3.4 GeV/c
- 2017
-
In: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 774, s. 252-257
-
Journal article (peer-reviewed)abstract
- Using a data set of 2.93 fb taken at a center-of-mass energy root s = 3.773 GeV with the BESIII detector at the BEPCII collider, we perform a search for an extra U(1) gauge boson, also denoted as a dark photon. We examine the initial state radiation reactions e(+)e(-) -> e(+)e(-) gamma(ISR) and e(+)e(-) -> mu(+)mu(-) gamma(ISR) for this search, where the dark photon would appear as an enhancement in the invariant mass distribution of the leptonic pairs. We observe no obvious enhancement in the mass range between 1.5 and 3.4 GeV/c(2) and set a 90% confidence level upper limit on the mixing strength of the dark photon and the Standard Model photon. We obtain a competitive limit in the tested mass range.
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| 11. |
- Ablikim, M., et al.
(author)
-
Evidence for e(+)e(-)->gamma chi c1,2 at center-of-mass energies from 4.009 to 4.360 GeV
- 2015
-
In: Chinese Physics C. - : IOP Publishing. - 1674-1137 .- 2058-6132. ; 39:4
-
Journal article (peer-reviewed)abstract
- Using data samples collected at center-of-mass energies of root s=4.009, 4.230, 4.260, and 4.360 GeV with the BESIII detector operating at the BEPCII collider, we perform a search for the process e(+)e(-)->gamma chi(cJ) (J=0, 1, 2) and find evidence for e(+)e(-)->gamma chi(c1) and e(+)e(-)->gamma chi(c2) with statistical significances of 3.0 sigma and 3.4 sigma, respectively. The Born cross sections sigma(B)(e(+)e(-)->gamma chi(cJ)), as well as their upper limits at the 90% confidence level (C.L.) are determined at each center-of-mass energy.
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| 12. |
- Ablikim, M., et al.
(author)
-
Measurement of B(psi(3770) -> gamma chi(c1)) and search for psi(3770) -> gamma chi(c2)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:9
-
Journal article (peer-reviewed)abstract
- We report a measurement of the branching fraction for psi(3770) -> gamma chi(c1) and search for the transition psi(3770) -> gamma chi(c2) based on 2.92 fb(-1) of e(+)e(-) data accumulated at root s = 3.773 GeV with the BESIII detector at the BEPCII collider. We measure B(psi(3770) -> gamma chi(c1)) = (2.48 +/- 0.15 +/- 0.23) x 10(-3), which is the most precise measurement to date. The upper limit on the branching fraction of psi(3770) -> gamma chi(c2) at a 90% confidence level is B(psi(3770) -> gamma chi(c2)) < 0.64 x 10(-3). The corresponding partial widths are Gamma(psi(3770) -> gamma chi(c1)) = (67.5 +/- 4.1 +/- 6.7)keV and Gamma(psi(3770) -> gamma chi(c2)) < 17.4 keV.
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| 13. |
- Ablikim, M., et al.
(author)
-
Measurement of the branching fractions of D-s(+) -> eta ' X and D-s(+) -> eta 'rho(+) in e(+)e(-) -> Ds+Ds-
- 2015
-
In: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 750, s. 466-474
-
Journal article (peer-reviewed)abstract
- We study D-s(+) decays to final states involving the eta' with a 482 pb(-1) data sample collected at root s = 4.009 GeV with the BESIII detector at the BEPCII collider. We measure the branching fractions B(D-s(+) -> eta'X) = (8.8 +/- 1.8 +/- 0.5)% and B(D-s(+) > eta'rho(+)) = (5.8 +/- 1.4 +/- 0.4)% where the first uncertainty is statistical and the second is systematic. In addition, we estimate an upper limit on the non-resonant branching ratio B(D-s(+) -> eta'pi(+)pi(0)) < 5.1% at the 90% confidence level. Our results are consistent with CLEO's recent measurements and help to resolve the disagreement between the theoretical prediction and CLEO's previous measurement of B(D-s(+) -> eta'rho(+)).
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| 14. |
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| 15. |
- Ablikim, M., et al.
(author)
-
Measurement of the leptonic decay width of J/psi using initial state radiation
- 2016
-
In: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 761, s. 98-103
-
Journal article (peer-reviewed)abstract
- Using a data set of 2.93 fb(-1) taken at a center-of-mass energy of root s = 3.773 GeV with the BESIII detector at the BEPCII collider, we measure the process e(+) e(-) -> J/psi gamma -> mu(+)mu(-)gamma and determine the product of the branching fraction and the electronic width B-mu mu . Gamma(ee) = (333.4 +/- 2.5(stat) +/- 4.4(sys)) eV. Using the earlier-published BESIII result for B-mu mu = (5.973 +/- 0.007(stat) +/- 0.037(sys))%, we derive the J/psi electronic width Gamma(ee) = (5.58 +/- 0.05(stat) +/- 0.08(sys)) keV. (C) 2016 The Author(s). Published by Elsevier B.V.
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| 16. |
- Ablikim, M., et al.
(author)
-
Measurement of the matrix elements for the decays eta -> pi(+)pi(-)pi(0) and eta/eta ' -> pi(0)pi(0)pi(0)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:1
-
Journal article (peer-reviewed)abstract
- Based on a sample of 1.31 x 10(9) J/psi events collected with the BESIII detector at the BEPCII collider, Dalitz plot analyses of selected 79,625 eta -> pi(+)pi(-)pi(0) events, 33,908 eta -> pi(0)pi(0)pi(0) events, and 1,888 eta' -> pi(0)pi(0)pi(0) events are performed. The measured matrix elements of eta -> pi(+)pi(-)pi(0) are in reasonable agreement with previous measurements. The Dalitz plot slope parameters of eta -> pi(0)pi(0)pi(0) and eta' -> pi(0)pi(0)pi(0) are determined to be -0.055 +/- 0.014 +/- 0.004 and -0.640 +/- 0.046 +/- 0.047, respectively, where the first uncertainties are statistical and the second systematic. Both values are consistent with previous measurements, while the precision of the latter one is improved by a factor of 3. Final state interactions are found to have an important role in those decays.
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| 17. |
- Ablikim, M., et al.
(author)
-
Measurement of the proton form factor by studying e(+)e(-) -> p(p)over-tilde
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:11
-
Journal article (peer-reviewed)abstract
- Using data samples collected with the BESIII detector at the BEPCII collider, we measure the Born cross section of e(+)e(-) -> p (p) over tilde at 12 center-of-mass energies from 2232.4 to 3671.0 MeV. The corresponding effective electromagnetic form factor of the proton is deduced under the assumption that the electric and magnetic form factors are equal (vertical bar G(E)vertical bar = vertical bar G(M)vertical bar). In addition, the ratio of electric to magnetic form factors, vertical bar G(E)/G(M)vertical bar, and vertical bar G(M)vertical bar are extracted by fitting the polar angle distribution of the proton for the data samples with larger statistics, namely at root s = 2232.4 and 2400.0 MeV and a combined sample at root s = 3050.0, 3060.0 and 3080.0 MeV, respectively. The measured cross sections are in agreement with recent results from BABAR, improving the overall uncertainty by about 30%. The vertical bar G(E)/G(M)vertical bar ratios are close to unity and consistent with BABAR results in the same q(2) region, which indicates the data are consistent with the assumption that vertical bar G(E)vertical bar = vertical bar G(M)vertical bar within uncertainties.
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| 18. |
- Ablikim, M., et al.
(author)
-
Measurement of y(CP) in D-0-(D)over-bar(0) oscillation using quantum correlations in e(+)e(-) -> D-0(D)over-bar(0) at root s=3.773 GeV
- 2015
-
In: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 744, s. 339-346
-
Journal article (peer-reviewed)abstract
- We report a measurement of the parameter y(CP) in D-0-(D) over bar (0) oscillations performed by taking advantage of quantum coherence between pairs of D-0(D) over bar (0) mesons produced in e(+)e(-) annihilations near threshold. In this work, doubly-tagged D-0(D) over bar (0) events, where one D decays to a CP eigenstate and the other D decays in a semileptonic mode, are reconstructed using a data sample of 2.92 fb(-1) collected with the BESIII detector at the center-of-mass energy of root s = 3.773 GeV. We obtain y(CP) = (-2.0 +/- 1.3 +/- 0.7)%, where the first uncertainty is statistical and the second is systematic. This result is compatible with the current world average.
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| 19. |
- Ablikim, M., et al.
(author)
-
Measurements of psi(3686) -> K-Lambda(Xi)over-bar(+) + c.c. and psi(3686) -> gamma K-Lambda(Xi)over-bar(+) + c.c.
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:9
-
Journal article (peer-reviewed)abstract
- Using a sample of 1.06 x 10(8) psi(3686) events produced in e(+)e(-) collisions at root s = 3.686 GeV and collected with the BESIII detector at the BEPCII collider, we present studies of the decays psi(3686) -> K-Lambda(Xi) over bar (+) + c.c. and psi(3686) -> gamma K-Lambda(Xi) over bar (+) + c.c. We observe two hyperons, Xi(1690)(-) and Xi(1820)(-), in the K-Lambda invariant mass distribution in the decay psi(3686) -> K-Lambda(Xi) over bar (+) + c.c. with significances of 4.9 sigma and 6.2 sigma, respectively. The branching fractions of psi(3686) -> K-Lambda(Xi) over bar (+) + c.c., psi(3686) -> K-Sigma(0)(Xi) over bar (+) + c.c, psi(3686) -> gamma chi cJ -> gamma K-Lambda(Xi) over bar (+) + c.c (J = 0, 1, 2), and psi(3686) -> Xi(1690/1820)(-)(Xi) over bar (+) + c.c with sub-sequent decay Xi(1690/1820)(-) -> K-Lambda are measured for the first time.
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| 20. |
- Ablikim, M., et al.
(author)
-
Observation and Spin-Parity Determination of the X(1835) in J/psi -> gamma(KSKS0)-K-0 eta
- 2015
-
In: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 115:9
-
Journal article (peer-reviewed)abstract
- We report an observation of the process J/psi -> gamma X(1835) -> gamma(KSKS0)-K-0 eta at low (KSKS0)-K-0 mass with a statistical significance larger than 12.9s using a data sample of 1.31 x 109 J/psi events collected with the BESIII detector. In this region of phase space the (KSKS0)-K-0 system is dominantly produced through the f (0)(980). By performing a partial wave analysis, we determine the spin parity of the Xd1835_ to be J(PC) = 0(-+). The mass and width of the observed X(1835) are 1844 +/- 9(stat)(-25)(+16)(syst) MeV/c(2) and 192(-17)(+20)(sta)(-43)(+62)(syst) MeV, respectively, which are consistent with the results obtained by BESIII in the channel J/psi -> gamma pi(+)pi(-)eta'.
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| 21. |
- Ablikim, M., et al.
(author)
-
Observation of a Neutral Charmoniumlike State Z(c)(4025)(0) in e(+)e(-) -> (D*(D)over-bar*)(0)pi(0)
- 2015
-
In: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 115:18
-
Journal article (peer-reviewed)abstract
- We report a study of the process e(+)e(-) -> (D*(D) over bar*)(0)pi(0) using e(+)e(-) collision data samples with integrated luminosities of 1092 pb(-1) at root s = 4.23 GeV and 826 pb(-1) at root s = 4.26 GeV collected with the BESIII detector at the BEPCII storage ring. We observe a new neutral structure near the (D*(D) over bar*)(0) mass threshold in the pi(0) recoil mass spectrum, which we denote as Z(c)(4025)(0). Assuming a Breit-Wigner line shape, its pole mass and pole width are determined to be (4025.5(-4.7)(+2.0) +/- 3.1) MeV/c(2) and (23.0 +/- 6.0 +/- 1.0) MeV, respectively. The Born cross sections of e(+)e(-) -> Z(c)(4025)(0)pi(0) -> (D*(D) over bar*)(0)pi(0) are measured to be (61.6 +/- 8.2 +/- 9.0) pb at root s = 4.23 GeV and (43.4 +/- 8.0 +/- 5.4) pb at root s = 4.26 GeV. The first uncertainties are statistical and the second are systematic.
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| 22. |
- Ablikim, M., et al.
(author)
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Observation of e(+)e(-) -> pi(0)pi(0)h(c) and a Neutral Charmoniumlike Structure Z(c)(4020)(0)
- 2014
-
In: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 113:21, s. 212002-
-
Journal article (peer-reviewed)abstract
- Using data collected with the BESIII detector operating at the Beijing Electron Positron Collider at center-of-mass energies of root s = 4.23, 4.26, and 4.36 GeV, we observe e(+)e(-) -> pi(0)pi(0)h(c) for the first time. The Born cross sections are measured and found to be about half of those of e(+)e(-) -> pi(+)pi(-)h(c) within less than 2 sigma. In the pi(0)h(c) mass spectrum, a structure at 4.02 GeV/c(2) is found. It is most likely to be the neutral isospin partner of the Z(c)(4020)(+/-) observed in the process of e(+)e(-) -> pi(+)pi(-)h(c) being found. A fit to the pi(0)h(c) invariant mass spectrum, with the width of the Z(c)(4020)(0) fixed to that of its charged isospin partner and possible interferences with non-Z(c)(4020)(0) amplitudes neglected, gives a mass of (4023.9 +/- 2.2 +/- 3.8) MeV/c(2) for the Z(c)(4020)(0), where the first error is statistical and the second systematic.
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| 23. |
- Ablikim, M., et al.
(author)
-
Observation of the Dalitz decay eta ' -> gamma e(+)e(-)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:1
-
Journal article (peer-reviewed)abstract
- We report the first observation of the Dalitz decay eta' -> gamma e(+)e(-), based on a data sample of 1.31 billion J/psi events collected with the BESIII detector. The eta' mesons are produced via the J/psi -> gamma eta' decay process. The ratio (eta' -> gamma e(+)e(-))/Gamma (eta' -> gamma gamma) is measured to be (2.13 +/- 0.09(stat) +/- 0.07(sys)) x 10(-2). This corresponds to a branching fraction B(eta' -> gamma e(+)e(-)) = (4.69 +/- 0.20(stat) +/- 0.23(sys)) x 10(-4). The transition form factor is extracted and different expressions are compared to the measured dependence on the e(+)e(-) invariant mass. The results are consistent with the prediction of the vector meson dominance model.
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| 24. |
- Ablikim, M., et al.
(author)
-
Observation of the electromagnetic doubly OZI-suppressed decay J/psi -> phi pi(0)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:11
-
Journal article (peer-reviewed)abstract
- Using a sample of 1.31 billion J/psi events accumulated with the BESIII detector at the BEPCII collider, we report the observation of the decay J/psi -> phi pi(0), which is the first evidence for a doubly Okubo-Zweig-Iizuka suppressed electromagnetic J/psi decay. A clear structure is observed in the K+K- mass spectrum around 1.02 GeV/c(2), which can be attributed to interference between J/psi -> phi pi(0) and J/psi -> K+K- pi(0) decays. Due to this interference, two possible solutions are found. The corresponding measured values of the branching fraction of J/psi -> phi pi(0) are [2.94 +/- 0.16(stat) +/- 0.16(syst)] x 10(-6) and [1.24 +/- 0.33(stat) +/- 0.30(syst)] x 10(-7).
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| 25. |
- Ablikim, M., et al.
(author)
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Observation of the isospin-violating decay J/psi -> phi pi(0)f(0) (980)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:1
-
Journal article (peer-reviewed)abstract
- Using a sample of 1.31 x 10(9) J/psi events collected with the BESIII detector at the BEPCII collider, the decays J/psi -> phi pi(+)pi(-)pi(0) and J/psi -> phi pi(0)pi(0)pi(0) are investigated. The isospin- violating decay J/psi -> phi pi(0)f(0)(980) with f(0)(980)-> pi pi is observed for the first time. The width of the f(0)(980) obtained from the dipion mass spectrum is found to be much smaller than the world average value. In the pi(0)f(0)(980) mass spectrum, there is evidence of f(1)(1285) production. By studying the decay J/psi ->eta', the branching fractions of eta' -> pi(+)pi(-)pi(0) and eta' -> pi(0)pi(0)pi(0), as well as their ratio, are also measured.
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| 26. |
- Ablikim, M., et al.
(author)
-
Observation of the Psi(1(3)D(2)) State in e(+)e(-) -> pi(+)pi(-)gamma chi(c1) at BESIII
- 2015
-
In: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 115:1
-
Journal article (peer-reviewed)abstract
- We report the observation of the X(3823) state in the process e(+)e(-) -> pi(+)pi(-) X(3823) pi(+)pi(-)gamma chi(c1) with a statistical significance of 6.2 sigma, in data samples at center-of-mass energies root s = 4.230, 4.260, 4.360, 4.420, and 4.600 GeV collected with the BESIII detector at the BEPCII electron positron collider. The measured mass of the X(3823) state is (3821.7 +/- 1.3 +/- 0.7) MeV/c(2), where the first error is statistical and the second systematic, and the width is less than 16 MeV at the 90% confidence level. The products of the Born cross sections for e(+)e(-) -> pi(+)pi(-) X(3823) and the branching ratio B [X(3823) -> gamma X-c1c2] are also measured. These measurements are in good agreement with the assignment of the X(3823) state as the Psi(1(3)D(2)) charmonium state.
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| 27. |
- Ablikim, M., et al.
(author)
-
Observation of Z(c)(3900)(0) in e(+)e(-) -> pi(0)pi(0) J/Psi
- 2015
-
In: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 115:11
-
Journal article (peer-reviewed)abstract
- Using a data sample collected with the BESIII detector operating at the BEPCII storage ring, we observe a new neutral state Z(c)(3900)(0) with a significance of 10.4 sigma. The mass and width are measured to be 3894.8 +/- 2.3 +/- 3.2 MeV/c(2) and 29.6 +/- 8.2 +/- 8.2 MeV, respectively, where the first error is statistical and the second systematic. The Born cross section for e(+)e(-) -> pi(0)pi(0) J/Psi and the fraction of it attributable to pi(0)Z(c)(3900)(0) -> pi(0)pi(0) J/Psi in the range E-c.m. = 4.19-4.42 GeV are also determined. We interpret this state as the neutral partner of the four-quark candidate Z(c)(3900)(+/-).
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| 28. |
- Ablikim, M., et al.
(author)
-
Precision measurement of the D*(0) decay branching fractions
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368 .- 2470-0010. ; 91:3
-
Journal article (peer-reviewed)abstract
- Using 482 pb(-1) of data taken at root s = 4.009 GeV, we measure the branching fractions of the decays of D*(0) into D-0 pi(0) and D-0 gamma to be B(D*(0) -> D-0 pi(0)) = (65.5 +/- 0.8 +/- 0.5)% and B(D*(0) -> D0 gamma) = (34.5 +/- 0.8 +/- 0.5)%, respectively, by assuming that the D*(0) decays only into these two modes. The ratio of the two branching fractions is B(D*(0) -> D-0 pi(0))/B(D*(0) -> D-0 gamma) = 1.90 +/- 0.07 +/- 0.05, which is independent of the assumption made above. The first uncertainties are statistical and the second ones systematic. The precision is improved by a factor of 3 compared to the present world average values.
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| 29. |
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| 30. |
- Ablikim, M., et al.
(author)
-
Search for C-parity violation in J/psi -> gamma gamma and gamma phi
- 2014
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 90:9, s. 092002-
-
Journal article (peer-reviewed)abstract
- Using 1.06 x 10(8) psi(3686) events recorded in e(+)e(-) collisions at root s = 3.686 GeV with the BESIII at the BEPCII collider, we present searches for C-parity violation in J/psi -> gamma gamma and gamma phi decays via psi(3686) -> J/psi pi(+)pi(-). No significant signals are observed in either channel. Upper limits on the branching fractions are set to be B(J/psi -> gamma gamma) < 2.7 x 10(-7) and B(J/psi -> gamma phi) < 1.4 x 10(-6) at the 90% confidence level. The former is one order of magnitude more stringent than the previous upper limit, and the latter represents the first limit on this decay channel.
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| 31. |
- Ablikim, M., et al.
(author)
-
Search for D-0 -> gamma gamma and improved measurement of the branching fraction for D-0 -> pi(0)pi(0)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:11
-
Journal article (peer-reviewed)abstract
- Using 2.92 fb(-1) of electron-positron annihilation data collected at root s = 3.773 GeV with the BESIII detector, we report the results of a search for the flavor-changing neutral current process D-0 -> gamma gamma using a double-tag technique. We find no signal and set an upper limit at 90% confidence level for the branching fraction of B(D-0 -> gamma gamma < 3.8 x 10(-6). We also investigate D-0-meson decay into two neutral pions, obtaining a branching fraction of B(D-0 -> pi(0)pi(0)) = (8.24 +/- 0.21(stat) +/- 0.30(syst)) x 10(-4), the most precise measurement to date and consistent with the current world average.
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| 32. |
- Ablikim, M., et al.
(author)
-
Search for the isospin violating decay Y(4260)-> J/psi eta pi(0)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:1
-
Journal article (peer-reviewed)abstract
- Using data samples collected at center-of-mass energies of root s = 4.009, 4.226, 4.257, 4.358, 4.416, and 4.599 GeV with the BESIII detector operating at the BEPCII storage ring, we search for the isospin violating decay Y(4260)-> J/psi eta pi(0). No signal is observed, and upper limits on the cross section sigma(e(+)e(-) -> J/psi eta pi(0)) at the 90% confidence level are determined to be 3.6, 1.7, 2.4, 1.4, 0.9, and 1.9 pb, respectively.
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| 33. |
- Ablikim, M., et al.
(author)
-
Search for the weak decays J/psi -> D-s(()*()-) e(+)nu(e) + c.c.
- 2014
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 90:11, s. 112014-
-
Journal article (peer-reviewed)abstract
- Using a sample of 2.25 x 10(8) J/psi events collected with the BESIII detector at the BEPCII collider, we search for the J/psi semileptonic weak decay J/psi -> D-s(-) e(+)nu(e) +c.c. with a much higher sensitivity than previous searches. We also perform the first search for J/psi -> D-s(*-) e(+) nu(e) + c.c. No significant excess of a signal above background is observed in either channel. At the 90% confidence level, the upper limits are determined to be B(J/psi -> D-s(-) e(+) nu(e) + c.c.) < 1.3 x 10(-6) and B(J/psi -> D-s*(-) e(+) nu(e) + c.c.) < 1.8 x 10(-6), respectively. Both are consistent with Standard Model predictions.
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| 34. |
- Ablikim, M., et al.
(author)
-
Search for the Y(4140) via e(+)e(-) -> gamma phi J/psi at root s=4.23, 4.26 and 4.36 GeV
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:3
-
Journal article (peer-reviewed)abstract
- Using data samples collected at center-of-mass energies root s = 4.23, 4.26, and 4.36 GeV with the BESIII detector operating at the BEPCII storage ring, we search for the production of the charmoniumlike state Y(4140) through a radiative transition followed by its decay to phi J/psi. No significant signal is observed and upper limits on sigma[e(+)e(-) -> gamma Y(4140)] . B(Y(4140) -> phi J/psi at the 90% confidence level are estimated as 0.35, 0.28, and 0.33 pb at root s = 4.23, 4.26, and 4.36 GeV, respectively.
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| 35. |
- Ablikim, M., et al.
(author)
-
Search for Z(c)(3900)(+/-) -> omega pi(+/-)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:3
-
Journal article (peer-reviewed)abstract
- The decay Z(c)(3900)(+/-) -> omega pi(+/-) is searched for using data samples collected with the BESIII detector operating at the BEPCII storage ring at center-of-mass energies root s = 4.23 and 4.26 GeV. No significant signal for the Z(c)(3900)(+/-) is found, and upper limits at the 90% confidence level on the Born cross section for the process e(+)e(-) -> Z(c)(3900)(+/-) pi(-/+) -> omega pi(+)pi(-) are determined to be 0.26 and 0.18 pb at root s = 4.23 and 4.26 GeV, respectively.
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| 36. |
- Ablikim, M., et al.
(author)
-
Searches for isospin-violating transitions chi(c0,2) -> pi(0)eta(c)
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:11
-
Journal article (peer-reviewed)abstract
- We present the first upper-limit measurement of the branching fractions of the isospin-violating transitions chi(c0,2) -> pi(0)eta(c). The measurements are performed using 106 x 10(6) psi(3686) events accumulated with the BESIII detector at the BEPCII e(+)e(-) collider at a center-of-mass energy corresponding to the psi(3686) mass. We obtained upper limits on the branching fractions at a 90% confidence level of B(chi(c0) -> pi(0)eta(c)) < 1.6 x 10(-3) and B(chi(c2) -> pi(0)eta(c)) < 3.2 x 10(-3).
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| 37. |
- Ablikim, M., et al.
(author)
-
Study of chi(cJ) decaying into phi K*(892)(K)over-bar
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:11
-
Journal article (peer-reviewed)abstract
- Using a data sample of 106 million psi(3686) events collected with the BESIII detector operated at the BEPCII storage ring, we study for the first time the decays chi(cJ) -> phi(KSK +/-)-K-0 pi(-/+) and chi(cJ) -> phi K+K-pi(0) in the E1 radiative transition psi(3686) -> gamma chi(cJ). The decays are dominated by the three-body decay chi(cJ) -> phi K*(892)(K) over bar. We measure branching fractions for this reaction via the neutral and charged K*(892) and find them consistent with each other within the expectation of isospin symmetry. In the K (K) over bar pi invariant mass distribution, a structure near the K*(892)(K) over bar mass threshold is observed, and the corresponding mass and width are measured to be 1412 +/- 4(stat) +/- 8(sys) MeV/c(2) and Gamma = 84 +/- 12(stat) +/- 40(sys) MeV, respectively. The observed state favors an assignment to the h(1)(1380), considering its possible J(PC) and comparing its mass, width and decay mode to those reported in the Particle Data Group.
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| 38. |
- Ablikim, M., et al.
(author)
-
Study of e(+)e(-) -> omega chi(cJ) at Center of Mass Energies from 4.21 to 4.42 GeV
- 2015
-
In: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 114:9
-
Journal article (peer-reviewed)abstract
- Based on data samples collected with the BESIII detector at the BEPCII collider at nine center of mass energies from 4.21 to 4.42 GeV, we search for the production of e(+)e(-) -> omega chi(cJ) (J = 0, 1, 2). The process e(+)e(-) -> omega chi(c0) is observed for the first time, and the Born cross sections at root s = 4.23 and 4.26 GeV are measured to be (55.4 +/- 6.0 +/- 5.9) and (23.7 +/- 5.3 +/- 3.5) pb, respectively, where the first uncertainties are statistical and the second are systematic. The omega chi(c0) signals at the other seven energies and the e(+)e(-) -> omega chi(c1) and omega chi(c2) signals are not significant, and the upper limits on the cross sections are determined. By examining the omega chi(c0) cross section as a function of center of mass energy, we find that it is inconsistent with the line shape of the Y(4260) observed in e(+)e(-) -> pi(+)pi(-) J/psi Assuming the omega chi(c0) signals come from a single resonance, we extract the mass and width of the resonance to be (4230 +/- 8 +/- 6) MeV/c(2) and (38 +/- 12 +/- 2) MeV, respectively, and the statistical significance is more than 9 sigma.
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| 39. |
- Ablikim, M., et al.
(author)
-
Study of J/psi -> eta phi pi(+)pi(-) at BESIII
- 2015
-
In: Physical Review D. - 1550-7998 .- 1550-2368. ; 91:5
-
Journal article (peer-reviewed)abstract
- invariant mass spectrum of phi f(0)(980) with a statistical significance of greater than 10 sigma. The corresponding mass and width are determined to be M = 2200 +/- 6(stat) +/- 5(syst) MeV/c(2) and Gamma = 104 +/- 15(stat) +/- 15(syst) MeV, respectively, and the product branching fraction is measured to be B(J/psi ->eta Y(2175), Y(2175)->phi f(0)(980), f(0)(980)->pi(+)pi(-)) = (1.20 +/- 0.14(stat)+/- 0.37(syst))x10(-4). The results are consistent within errors with those of previous experiments. We also measure the branching fraction of J/psi ->phi f(1)(1285) with f(1)(1285)->eta pi(+)pi(-) and set upper limits on the branching fractions for J/psi ->phi eta(1405)/phi X(1835)/phi X(1870) with eta(1405)/X(1835)/X(1870)->eta pi(+)pi(-) at the 90% confidence level.
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| 40. |
- Liang, Yusen, et al.
(author)
-
Migrating photon avalanche in different emitters at the nanoscale enables 46th-order optical nonlinearity
- 2022
-
In: Nature Nanotechnology. - : Springer Nature. - 1748-3387 .- 1748-3395. ; 17:5, s. 524-530
-
Journal article (peer-reviewed)abstract
- A photon avalanche (PA) effect that occurs in lanthanide-doped solids gives rise to a giant nonlinear response in the luminescence intensity to the excitation light intensity. As a result, much weaker lasers are needed to evoke such PAs than for other nonlinear optical processes. Photon avalanches are mostly restricted to bulk materials and conventionally rely on sophisticated excitation schemes, specific for each individual system. Here we show a universal strategy, based on a migrating photon avalanche (MPA) mechanism, to generate huge optical nonlinearities from various lanthanide emitters located in multilayer core/shell nanostructrues. The core of the MPA nanoparticle, composed of Yb3+ and Pr3+ ions, activates avalanche looping cycles, where PAs are synchronously achieved for both Yb3+ and Pr3+ ions under 852 nm laser excitation. These nanocrystals exhibit a 26th-order nonlinearity and a clear pumping threshold of 60 kW cm−2. In addition, we demonstrate that the avalanching Yb3+ ions can migrate their optical nonlinear response to other emitters (for example, Ho3+ and Tm3+) located in the outer shell layer, resulting in an even higher-order nonlinearity (up to the 46th for Tm3+) due to further cascading multiplicative effects. Our strategy therefore provides a facile route to achieve giant optical nonlinearity in different emitters. Finally, we also demonstrate applicability of MPA emitters to bioimaging, achieving a lateral resolution of ~62 nm using one low-power 852 nm continuous-wave laser beam.
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| 41. |
- Ling, Yiming, et al.
(author)
-
Observations of Kelvin-Helmholtz Waves in the Earth's Magnetotail Near the Lunar Orbit
- 2018
-
In: Journal of Geophysical Research - Space Physics. - 2169-9380 .- 2169-9402. ; 123:5, s. 3836-3847
-
Journal article (peer-reviewed)abstract
- Kelvin‐Helmholtz waves (KHWs), which have been widely observed at the magnetopause in the region near the Earth, play an essential role in the transport of solar wind plasma and energy into the magnetosphere under dominantly northward interplanetary magnetic field (IMF) conditions. In this study, we present simultaneous observations of KHWs under the northward IMF observed by both the Acceleration, Reconnection, Turbulence, and Electrodynamics of Moon's Interaction with the Sun (ARTEMIS) spacecraft in the Earth's magnetotail around the lunar orbit (at X ~ −50RE, Y ~ 30RE, dusk side) and the Geotail in the near‐Earth space (at X ~ −5RE, Y ~ −10RE, dawn side). The KHWs are quantitatively characterized by their dominant period, phase velocity, and wavelength, utilizing wavelet analysis and an approximation of their center‐of‐mass velocity. Our results suggest that the phase velocity and spatial scale of KHWs may increase as they propagate along the boundary layer toward the tail. Alternatively, the differences between the ARTEMIS and Geotail observations may indicate the possibility of dawn‐dusk asymmetry in the excited KHWs in this study. Our results strongly evidence the existence of the development of KHWs in terms of their wave frequency and scale size in the magnetotail and provide insight to the time evolution of KHWs along the magnetopause.
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| 42. |
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| 43. |
- Pecunia, Vincenzo, et al.
(author)
-
Roadmap on energy harvesting materials
- 2023
-
In: Journal of Physics. - : IOP Publishing. - 2515-7639. ; 6:4
-
Journal article (peer-reviewed)abstract
- Ambient energy harvesting has great potential to contribute to sustainable development and address growing environmental challenges. Converting waste energy from energy-intensive processes and systems (e.g. combustion engines and furnaces) is crucial to reducing their environmental impact and achieving net-zero emissions. Compact energy harvesters will also be key to powering the exponentially growing smart devices ecosystem that is part of the Internet of Things, thus enabling futuristic applications that can improve our quality of life (e.g. smart homes, smart cities, smart manufacturing, and smart healthcare). To achieve these goals, innovative materials are needed to efficiently convert ambient energy into electricity through various physical mechanisms, such as the photovoltaic effect, thermoelectricity, piezoelectricity, triboelectricity, and radiofrequency wireless power transfer. By bringing together the perspectives of experts in various types of energy harvesting materials, this Roadmap provides extensive insights into recent advances and present challenges in the field. Additionally, the Roadmap analyses the key performance metrics of these technologies in relation to their ultimate energy conversion limits. Building on these insights, the Roadmap outlines promising directions for future research to fully harness the potential of energy harvesting materials for green energy anytime, anywhere.
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| 44. |
- Shen, Qian, et al.
(author)
-
The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis
- 2018
-
In: Molecular Plant. - : Cell Press. - 1674-2052 .- 1752-9867. ; 11:6, s. 776-788
-
Journal article (peer-reviewed)abstract
- Artemisia annua, commonly known as sweet wormwood or Qinghao, is a shrub native to China and has long been used for medicinal purposes. A. annua is now cultivated globally as the only natural source of a potent anti-malarial compound, artemisinin. Here, we report a high-quality draft assembly of the 1.74-gigabase genome of A. annua, which is highly heterozygous, rich in repetitive sequences, and contains 63 226 protein-coding genes, one of the largest numbers among the sequenced plant species. We found that, as one of a few sequenced genomes in the Asteraceae, the A. annua genome contains a large number of genes specific to this large angiosperm clade. Notably, the expansion and functional diversification of genes encoding enzymes involved in terpene biosynthesis are consistent with the evolution of the artemisinin biosynthetic pathway. We further revealed by transcriptome profiling that A. annua has evolved the sophisticated transcriptional regulatory networks underlying artemisinin biosynthesis. Based on comprehensive genomic and transcriptomic analyses we generated transgenic A. annua lines producing high levels of artemisinin, which are now ready for large-scale production and thereby will help meet the challenge of increasing global demand of artemisinin.
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| 45. |
- Wang, Hao, et al.
(author)
-
In-situ growth of low-dimensional perovskite-based insular nanocrystals for highly efficient light emitting diodes
- 2023
-
In: Light. - : SPRINGERNATURE. - 2095-5545 .- 2047-7538. ; 12:1
-
Journal article (peer-reviewed)abstract
- Regulation of perovskite growth plays a critical role in the development of high-performance optoelectronic devices. However, judicious control of the grain growth for perovskite light emitting diodes is elusive due to its multiple requirements in terms of morphology, composition, and defect. Herein, we demonstrate a supramolecular dynamic coordination strategy to regulate perovskite crystallization. The combined use of crown ether and sodium trifluoroacetate can coordinate with A site and B site cations in ABX(3) perovskite, respectively. The formation of supramolecular structure retard perovskite nucleation, while the transformation of supramolecular intermediate structure enables the release of components for slow perovskite growth. This judicious control enables a segmented growth, inducing the growth of insular nanocrystal consist of low-dimensional structure. Light emitting diode based on this perovskite film eventually brings a peak external quantum efficiency up to 23.9%, ranking among the highest efficiency achieved. The homogeneous nano-island structure also enables high-efficiency large area (1 cm(2)) device up to 21.6%, and a record high value of 13.6% for highly semi-transparent ones.
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| 46. |
- Zhang, Ya Hong, et al.
(author)
-
AβPP-tau-HAS1 axis trigger HAS1-related nuclear speckles and gene transcription in Alzheimer's disease
- 2024
-
In: Matrix Biology. - 0945-053X. ; 129, s. 29-43
-
Journal article (peer-reviewed)abstract
- As the backbone of the extracellular matrix (ECM) and the perineuronal nets (PNNs), hyaluronic acid (HA) provides binding sites for proteoglycans and other ECM components. Although the pivotal of HA has been recognized in Alzheimer's disease (AD), few studies have addressed the relationship between AD pathology and HA synthases (HASs). Here, HASs in different regions of AD brains were screened in transcriptomic database and validated in AβPP/PS1 mice. We found that HAS1 was distributed along the axon and nucleus. Its transcripts were reduced in AD patients and AβPP/PS1 mice. Phosphorylated tau (p-tau) mediates AβPP-induced cytosolic-nuclear translocation of HAS1, and negatively regulated the stability, monoubiquitination, and oligomerization of HAS1, thus reduced the synthesis and release of HA. Furthermore, non-ubiquitinated HAS1 mutant lost its enzyme activity, and translocated from the cytosol into the nucleus, forming nuclear speckles (NS). Unlike the splicing-related NS, less than 1 % of the non-ubiquitinated HAS1 co-localized with SRRM2, proving the regulatory role of HAS1 in gene transcription, indirectly. Thus, differentially expressed genes (DEGs) related to both non-ubiquitinated HAS1 mutant and AD were screened using transcriptomic datasets. Thirty-nine DEGs were identified, with 64.1 % (25/39) showing consistent results in both datasets. Together, we unearthed an important function of the AβPP-p-tau-HAS1 axis in microenvironment remodeling and gene transcription during AD progression, involving the ubiquitin-proteasome, lysosome, and NS systems.
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