| 1. |
- Boffano, P., et al.
(author)
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The epidemiology and management of ameloblastomas: A European multicenter study
- 2021
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In: Journal of Cranio-Maxillofacial Surgery. - : Elsevier BV. - 1010-5182. ; 49:12, s. 1107-1112
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Journal article (peer-reviewed)abstract
- The present study aimed at assessing the epidemiology including demographic variables, diagnostic features, and management of ameloblastomas at several European departments of maxillofacial and oral surgery. The following data were recorded for each patient: gender, age, voluptuary habits, comorbidities, site, size, radiographic features, type, histopathological features, kind of treatment, length of hospital stay, complications, recurrence, management and complications of the recurrence. A total of 244 patients, 134 males and 110 females with ameloblastomas were included in the study. Mean age was 47.4 years. In all, 81% of lesions were found in the mandible, whereas 19% were found in the maxilla. Mean size of included ameloblastomas was 38.9 mm. The most frequently performed treatment option was enucleation plus curettage/peripheral ostectomy in 94 ameloblastomas, followed by segmental resection (60 patients), simple enucleation (46 patients), and marginal resection (40 pa-tients). A recurrence (with a mean follow up of 5 years) was observed in 47 cases out of 244 ameloblastomas (19.3%). Segmental resection was associated with a low risk of recurrence (p = 0003), whereas enucleation plus curettage/peripheral ostectomy was associated with a high risk of recurrence (p = 0002). A multilocular radiographic appearance was associated with a high risk of recurrence (p < .05), as well as the benign solid/multicystic histologic type (p < .05). Within the limitations of the study it seems that the management of ameloblastomas will probably remain controversial even in the future. Balancing low surgical morbidity with a low recurrence rate is a difficult aim to reach. (c) 2021 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
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| 2. |
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| 3. |
- Berggren, Magnus, et al.
(author)
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Light-emitting diodes with variable colours from polymer blends
- 1994
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In: Nature. - : Nature Publishing Group. - 0028-0836 .- 1476-4687. ; 372:6505, s. 444-446
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Journal article (peer-reviewed)abstract
- THE range of materials now available for polymer-based light-emitting diodes (LEDs) is such that electroluminescence can be obtained throughout the visible spectrum(1-12). Here we show that, by blending polymers with different emission and charge-transport characteristics, LEDs can be fabricated in which the emission colour varies as a function of the operating voltage. This phenomenon arises from the self-organizing properties of the blends, in which entropy drives phase separation of the constituent polymers and gives rise to submicrometre-sized domains having a range of compositions and emission characteristics. Emission from domains of different composition is controlled by the ease with which charge is injected, which in turn depends on the applied voltage.
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| 4. |
- Ehrenfest, D. M. D., et al.
(author)
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In Search of a Consensus Terminology in the Field of Platelet Concentrates for Surgical Use: Platelet-Rich Plasma (PRP), Platelet-Rich Fibrin (PRF), Fibrin Gel Polymerization and Leukocytes
- 2012
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In: Current Pharmaceutical Biotechnology. - 1389-2010. ; 13:7, s. 1131-1137
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Journal article (peer-reviewed)abstract
- In the field of platelet concentrates for surgical use, most products are termed Platelet-Rich Plasma (PRP). Unfortunately, this term is very general and incomplete, leading to many confusions in the scientific database. In this article, a panel of experts discusses this issue and proposes an accurate and simple terminology system for platelet concentrates for surgical use. Four main categories of products can be easily defined, depending on their leukocyte content and fibrin architecture: Pure Platelet-Rich Plasma (P-PRP), such as cell separator PRP, Vivostat PRF or Anitua's PRGF; Leukocyte- and Platelet-Rich Plasma (L-PRP), such as Curasan, Regen, Plateltex, SmartPReP, PCCS, Magellan, Angel or GPS PRP; Pure Plaletet-Rich Fibrin (P-PRF), such as Fibrinet; and Leukocyte-and Platelet-Rich Fibrin (L-PRF), such as Choukroun's PRF. P-PRP and L-PRP refer to the unactivated liquid form of these products, their activated versions being respectively named P-PRP gels and L-PRP gels. The purpose of this search for a terminology consensus is to plead for a more serious characterization of these products. Researchers have to be aware of the complex nature of these living biomaterials, in order to avoid misunderstandings and erroneous conclusions. Understanding the biomaterials or believing in the magic of growth factors ? From this choice depends the future of the field.
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| 5. |
- Holm, Åsa, et al.
(author)
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Protein C α regulates phagocytosis actin dynamics and phagosomal maturation in macrophages
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Other publication (other academic/artistic)abstract
- Protein kinase C α (PKCα) participates in F-actin remodeling during phagocytosis and phagosomal maturation in macrophages. Promastigotes of the protozoan parasite Leishmania donovani cause an accumulation of periphagosomal F-actin instead of the normal decrease seen with other prey [1]. This accumulation is dependent on promastigote lipophosphoglycan (LPG), which has several detrimental effects on the cell including inhibition of PKCα activity.To directly address the role of PKCα and LPG for actin remodeling in macrophages, we investigated F-actin dynamics in RAW 264.7 macrophages overexpressing a dominant-negative mutant of PKCα (DN PKCα). We found that DN PKCα-overexpressing cells displayed increased levels of cortical F-actin and decreased phagocytic capacity, which was augmented when the cells were subjected to LPG-coated prey. The DN PKCα-overexpressing cells also showed defective breakdown of periphagosomal F-actin and inhibition of phagosomal maturation. The level of periphagosomal F-actin was similar to that of controls subjected to LPG-coated prey. Our results show that PKCα regulates phagocytosis and F-actin turnover in macrophages, and that PKCα-dependent breakdown of periphagosomal F-actin is required for normal phagosomal maturation.
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| 6. |
- Holm, Åsa, et al.
(author)
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Role of protein kinase C α for uptake of unopsonized prey and phagosomal maturation in macrophages
- 2003
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In: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 302:4, s. 653-658
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Journal article (peer-reviewed)abstract
- Protein kinase C α (PKCα) participates in F-actin remodeling during phagocytosis and phagosomal maturation in macrophages. Leishmania donovani promastigotes, which inhibit phagosomal maturation, cause accumulation of periphagosomal F-actin instead of the dissassembly observed around other prey [Cell. Microbiol. 7 (2001) 439]. This accumulation is induced by promastigote lipophosphoglycan (LPG), which has several effects on macrophages including inhibition of PKCα. To investigate a possible connection between PKCα and LPG’s effects on actin dynamics, we utilized RAW264.7 macrophages overexpressing dominant-negative PKCα (DN PKCα). We found increased cortical F-actin and decreased phagocytic capacity, as well as defective periphagosomal F-actin breakdown and inhibited phagosomal maturation in the DN PKCα-overexpressing cells, effects similar to those seen in controls subjected to LPG-coated prey. The results indicate that PKCα is involved in F-actin turnover in macrophages and that PKCα-dependent breakdown of periphagosomal F-actin is required for phagosomal maturation, and endorse the hypothesis that intracellular survival of L. donovani involves inhibition of PKCα by LPG.
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| 7. |
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| 8. |
- Ramirez-Aguilar, Santiago J., et al.
(author)
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The Composition of Plant Mitochondrial Supercomplexes Changes with Oxygen Availability
- 2011
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In: Journal of Biological Chemistry. - 1083-351X. ; 286:50, s. 43045-43053
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Journal article (peer-reviewed)abstract
- Background: Respiratory supercomplexes are known to exist, but their function remains to be revealed. Results: Plant supercomplexes are affected by hypoxia and a concomitant drop in pH. Conclusion: Respiratory supercomplexes are dynamic structures that are affected by the intracellular environment. Significance: Supercomplexes could have a regulatory function in guiding electrons through alternative respiratory pathways.
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| 9. |
- Rasmusson, Allan, et al.
(author)
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Alternative oxidase: a defence against metabolic fluctuations?
- 2009
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In: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 137, s. 371-382
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Journal article (peer-reviewed)abstract
- An increasing number of oscillating or fluctuating cellular systems have been recently described following the adaptation of fluorescent technology. In diverse organisms, these variously involve signalling factors, heat production, central metabolism and reactive oxygen species (ROS). In response to many plant stresses and primarily via the influence of ROS, changes in mRNA and protein levels or in vivo activity of alternative oxidase are often observed. However, in several investigations, a lack of correlation between the mRNA, protein and in vivo activity has been evident. This discrepancy has made it questionable whether the induction of alternative oxidase has importance in regulating alternative pathway activity in vivo, or being diagnostic for a role of alternative oxidase in stress tolerance and ROS avoidance. Here, we suggest a role of alternative oxidase in counteracting deleterious short-term metabolic fluctuations, especially under stress conditions. This model emphasizes the importance of peak activity for establishing protein levels and allows an amalgamation of the present status of physiological, cellular and molecular knowledge.
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| 10. |
- Rasmusson, A. G., et al.
(author)
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Isolation of the rotenome-sensitive NADH-ubiquinone reductase (complex I) from red beet mitochondria
- 1994
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In: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 90:3, s. 607-615
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Journal article (peer-reviewed)abstract
- Complex 1 of the respirator) chain (EC 1.6.531, measured as NADH-duroquinone and NADH-ubiquinone, reductase activities, was isolated from purified red beetroot (Beta vulgaris L.I mitochondria. The mitochondria were disrupted by freeze-thawing and inner membrane vesicles were pelleted. After solubilization of the vesicles with Triton X-100, the enzyme complex was purified 11-fold (compared to the activity in the inner membrane vesicles) by size-exclusion chromatography on a Sephacryl S-400 HR column and then by ion-exchange chromatography on a DEAE-Sepharose CL-6B column. Triton X-100 was present throughout the purification procedure. Tire purified complex showed approximately 30 bands on SDS-PAGE and about 15 polypeptides including those at 80. 54, 53. 51. 27. 25 and 22 kDa cross-reacted with polyclonal antibodies raised against complex I from Neurospora crassa. This is similar lo the pattern obtained with complex I from Neurospera crassa.Analysis by nativc-SDS 2-dimensional PAGE revealed the existence of several molecular mass forms of the purified complex.After reconstitution of the purified complex into phosphatidylcholine vesicles, the NADH-ubiquinone reductase activity had a Km (NADH) of about I μM and was inhibited by both rotenone and dicyclohexylcarbodiimide.
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| 11. |
- Rasmusson, J., et al.
(author)
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LORA - a new airborne imaging radar sensor
- 2005
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In: Proc. RVK 05, Radiovetenskap och kommunikation, Linköping, Sweden, June 14-16 2005. ; , s. 531-534
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Conference paper (other academic/artistic)
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| 12. |
- Rasmusson, J.R., et al.
(author)
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Bistatic VHF and UHF SAR for urban environments
- 2007
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In: Proceedings of SPIE -- Volume 6547, Radar Sensor Technology XI, James L. Kurtz, Robert J. Tan, Editors, 654705, May 2, 2007. ; 6547
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Conference paper (other academic/artistic)
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| 13. |
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| 14. |
- Undeman, Emma, 1981-, et al.
(author)
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Micropollutants in urban wastewater : large-scale emission estimates and analysis of measured concentrations in the Baltic Sea catchment
- 2022
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In: Marine Pollution Bulletin. - : Elsevier BV. - 0025-326X .- 1879-3363. ; 178
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Journal article (peer-reviewed)abstract
- Wastewater treatment plants (WWTPs) transmit many chemical contaminants to aquatic environments. Quantitative data on micropollutant emissions via WWTPs are needed for environmental risk assessments and evaluation of mitigation measures. This study compiled published data on substances analysed in effluents from WWTPs in the Baltic Sea region, assessed country related differences in the data sets and estimated micropollutant inputs to the Baltic Sea catchment. Concentration data were found for 1090 substances analysed at 650 WWTPs. Heterogeneity and low number of data points for most substances hindered adequate comparisons of country specific concentrations. Emission estimates were made for the 280 substances analysed in at least five WWTPs in years 2010 to 2019. For selected substances, mass loads were compared to previously published estimations. The study provides data useful for national and Baltic Sea-scale pressure analysis and risk assessments. However, it also highlights the need for broad scope monitoring of micropollutants in wastewater.
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| 15. |
- Wrobel, L., et al.
(author)
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Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance
- 2022
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 13:1
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Journal article (peer-reviewed)abstract
- Enhancing the removal of aggregate-prone toxic proteins is a rational therapeutic strategy for a number of neurodegenerative diseases, especially Huntington's disease and various spinocerebellar ataxias. Ideally, such approaches should preferentially clear the mutant/misfolded species, while having minimal impact on the stability of wild-type/normally-folded proteins. Furthermore, activation of both ubiquitin-proteasome and autophagy-lysosome routes may be advantageous, as this would allow effective clearance of both monomeric and oligomeric species, the latter which are inaccessible to the proteasome. Here we find that compounds that activate the D1 ATPase activity of VCP/p97 fulfill these requirements. Such effects are seen with small molecule VCP activators like SMER28, which activate autophagosome biogenesis by enhancing interactions of PI3K complex components to increase PI(3)P production, and also accelerate VCP-dependent proteasomal clearance of such substrates. Thus, this mode of VCP activation may be a very attractive target for many neurodegenerative diseases. Several neurodegenerative diseases are characterized by the aggregation of cytoplasmic proteins. Here, the authors demonstrate that the small molecule SMER28 activates VCP, which enhances both autophagic and proteasomal clearance of aggregate-prone proteins.
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