| 1. |
- Beal, Jacob, et al.
(author)
-
Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
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| 4. |
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| 5. |
|
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| 6. |
|
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| 7. |
|
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| 8. |
|
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| 9. |
|
|
| 10. |
- Aad, G., et al.
(author)
-
- 2014
-
In: The European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6052. ; 74:8
-
Journal article (peer-reviewed)
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|
| 11. |
- Aad, G., et al.
(author)
-
- 2014
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In: The European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6052. ; 74:6
-
Journal article (peer-reviewed)
|
|
| 12. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
|
| 13. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Physical Review C (Nuclear Physics). - 0556-2813 .- 1089-490X. ; 90:2
-
Journal article (peer-reviewed)
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|
| 14. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
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| 15. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
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| 16. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
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| 17. |
- Aad, G., et al.
(author)
-
- 2014
-
swepub:Mat__t
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| 18. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Physical Review Letters. - 1079-7114 .- 0031-9007. ; 112:9
-
Journal article (peer-reviewed)
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|
| 19. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :9
-
Journal article (peer-reviewed)
|
|
| 20. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
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| 21. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :8
-
Journal article (peer-reviewed)
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| 22. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Physical Review D. Particles and fields. - 0556-2821 .- 1089-4918. ; 89:9
-
Journal article (peer-reviewed)
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| 23. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - : Springer. - 1029-8479 .- 1126-6708. ; :6
-
Journal article (peer-reviewed)
|
|
| 24. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :6
-
Journal article (peer-reviewed)
|
|
| 25. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :6
-
Journal article (peer-reviewed)
|
|
| 26. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :5
-
Journal article (peer-reviewed)
|
|
| 27. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
|
| 28. |
- Aad, G., et al.
(author)
-
- 2015
-
In: Journal of High Energy Physics. - : Springer-Verlag New York. - 1029-8479 .- 1126-6708. ; :9
-
Journal article (peer-reviewed)
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|
| 29. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Physical Review D (Particles, Fields, Gravitation and Cosmology). - 1550-2368 .- 1550-7998. ; 90:5
-
Journal article (peer-reviewed)
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|
| 30. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :9, s. 1-49
-
Journal article (peer-reviewed)
|
|
| 31. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
|
| 32. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :6
-
Journal article (peer-reviewed)
|
|
| 33. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :4
-
Journal article (peer-reviewed)
|
|
| 34. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :4
-
Journal article (peer-reviewed)
|
|
| 35. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :5, s. 1-52
-
Journal article (peer-reviewed)
|
|
| 36. |
- Aad, G., et al.
(author)
-
- 2013
-
Journal article (peer-reviewed)
|
|
| 37. |
- Aad, G., et al.
(author)
-
- 2013
-
Journal article (peer-reviewed)
|
|
| 38. |
- Aad, G., et al.
(author)
-
- 2013
-
Journal article (peer-reviewed)
|
|
| 39. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
|
| 40. |
- Aad, G., et al.
(author)
-
- 2014
-
In: Physical Review Letters. - 1079-7114 .- 0031-9007. ; 112:20
-
Journal article (peer-reviewed)
|
|
| 41. |
- Aad, G., et al.
(author)
-
- 2013
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :10
-
Journal article (peer-reviewed)
|
|
| 42. |
- Aad, G., et al.
(author)
-
- 2013
-
In: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :9
-
Journal article (peer-reviewed)
|
|
| 43. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
|
| 44. |
|
|
| 45. |
|
|
| 46. |
- Aad, G., et al.
(author)
-
- 2015
-
Journal article (peer-reviewed)
|
|
| 47. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
|
| 48. |
- Aad, G., et al.
(author)
-
- 2013
-
In: New Journal of Physics. - : IOP Publishing. - 1367-2630. ; 15
-
Journal article (peer-reviewed)
|
|
| 49. |
- Aad, G., et al.
(author)
-
- 2013
-
Journal article (peer-reviewed)
|
|
| 50. |
- Aad, G., et al.
(author)
-
- 2014
-
Journal article (peer-reviewed)
|
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