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- Singh, Abeer Prakash, 1988, et al.
(author)
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Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS Biogenesis
- 2020
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In: Molecular Cell. - : Elsevier BV. - 1097-2765 .- 1097-4164. ; 79:6
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Journal article (peer-reviewed)abstract
- Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BiolD, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved in transcription, RNA processing, translation, or protein biogenesis. Our analyses indicate the spatial organization of these processes, thereby revealing basic mechanistic principles and the proteins populating strategically important sites. For example, newly synthesized proteins are directly handed over to ribosomal tunnel exit-bound factors that mediate membrane insertion, co-factor acquisition, or their mounting into OXPHOS complexes in a special early assembly hub. Collectively, the data reveal the connectivity of mitochondrial gene expression, reflecting a unique tailoring of the mitochondrial gene expression system.
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| 2. |
- Salvatori, Roger, 1988, et al.
(author)
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Mapping protein networks in yeast mitochondria using proximity-dependent biotin identification coupled to proteomics
- 2020
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In: STAR PROTOCOLS. - : Elsevier BV. - 2666-1667. ; 1:3
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Journal article (peer-reviewed)abstract
- Proximity-dependent biotin identification (BioID) permits biotinylation of proteins interacting directly, indirectly, or just localized in proximity of a protein of interest (bait). Here, we describe how BioID coupled to proteomics and network biology can be used to map protein proximities in yeast mitochondria, aiding in visualization of complex protein-protein interaction landscapes. For complete information on the use and execution of this protocol, please refer to Singh et al., 2020.
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