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- Kupari, J, et al.
(author)
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Single cell transcriptomics of primate sensory neurons identifies cell types associated with chronic pain
- 2021
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In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 12:1, s. 1510-
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Journal article (peer-reviewed)abstract
- Distinct types of dorsal root ganglion sensory neurons may have unique contributions to chronic pain. Identification of primate sensory neuron types is critical for understanding the cellular origin and heritability of chronic pain. However, molecular insights into the primate sensory neurons are missing. Here we classify non-human primate dorsal root ganglion sensory neurons based on their transcriptome and map human pain heritability to neuronal types. First, we identified cell correlates between two major datasets for mouse sensory neuron types. Machine learning exposes an overall cross-species conservation of somatosensory neurons between primate and mouse, although with differences at individual gene level, highlighting the importance of primate data for clinical translation. We map genomic loci associated with chronic pain in human onto primate sensory neuron types to identify the cellular origin of chronic pain. Genome-wide associations for chronic pain converge on two different neuronal types distributed between pain disorders that display different genetic susceptibilities, suggesting both unique and shared mechanisms between different pain conditions.
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| 12. |
- Jalilehvand, F., et al.
(author)
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Hydration of the calcium ion. An EXAFS, large-angle X-ray scattering, and molecular dynamics simulation study
- 2001
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In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 123:3, s. 431-441
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Journal article (peer-reviewed)abstract
- The structure of the hydrated calcium(II) ion in aqueous solution has been studied by means of extended X-ray absorption fine structure spectroscopy (EXAFS), large-angle X-ray scattering (LAXS), and molecular dynamics (MD) methods. The EXAFS data displayed a broad and asymmetric distribution of the Ca-O bond distances with the centroid at 2.46(2) Angstrom. LAXS studies on four aqueous calcium halide solutions (1.5-2 mol dm(-3)) gave a mean Ca-O bond distance of 2.46(1) Angstrom. This is consistent with a hydration number of 8 determined from correlations between mean distances and coordination numbers from crystal structures. The LAXS studies showed a second coordination sphere with a mean Ca . . .O-II distance of 4.58(5) Angstrom, and for the hydrated halide ions the distances Cl . . .O 3.25(1) Angstrom. Br . . .O 3.36(1) Angstrom, and I . .O 3.61(1) Angstrom were obtained. Molecular dynamics simulations of CaCl2(aq) were performed using three different Ca2+-OH2 pair potentials. The potential from the GROMOS program gave results in agreement with experiments, i.e., a coordination number of 8 and an average Ca-O distance of 2.46 Angstrom, and was used for further comparisons. Theoretical EXAFS oscillations were computed far individual Mn snapshots and showed very large variations, though the simulated average spectrum from 2000 snapshots gave satisfactory agreement with the experimental EXAFS spectra. The effect of thermal motions of the coordinated atoms is inherent in the MD simulation method. Thermal disorder parameters evaluated from simulated spatial atom distribution functions of the oxygen atoms coordinated to the calcium ion were in close agreement with those from the current LAXS and EXAFS analyses. The combined results are consistent with a root-mean-square displacement from the mean Ca-O distance of 0.09(2) Angstrom in aqueous solution at 300 K.
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- Sandstrom, M., et al.
(author)
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Hydration of some large and highly charged metal ions
- 2001
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In: Journal of Synchrotron Radiation. - 0909-0495 .- 1600-5775. ; 8, s. 657-659
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Journal article (peer-reviewed)abstract
- EXAFS studies of metal ions with hydration numbers higher than six in aqueous solution, often show asymmetric distribution of the metal-oxygen bond distances. The hydration number can be determined from a correlation with the bond distance. The mean Ca-O distance 2.46(1) Angstrom shows the calcium( II) ion to be eight-hydrated in a wide asymmetric distribution. Theoretically calculated EXAFS oscillations for individual snapshots from an MD simulation show large variations. The scandium( III) ion is surrounded by two groups of about eight water molecules, with the mean Sc-O distance 2.185(6) Angstrom. The yttrium( III) ion coordinates eight waters in an asymmetric distribution at 2.368(5) Angstrom, and the lanthanum( III) ion 6 + 3 water molecules at 2.52(2) and 2.65(3) Angstrom, respectively. For the the uranium( IV) and thorium(IV) ions, the M-O distances 2.42(1) and 2.45(1) Angstrom, respectively, indicate hydration numbers close to 10.
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| 17. |
- Schafer, Werner, et al.
(author)
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Good urodynamic practices: uroflowmetry, filling cystometry, and pressure-flow studies
- 2002
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In: Neurourology and Urodynamics. - : Wiley. - 0733-2467. ; 21:3, s. 261-274
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Journal article (peer-reviewed)abstract
- This is the first report of the International Continence Society (ICS) on the development of comprehensive guidelines for Good Urodynamic Practice for the measurement, quality control, and documentation of urodynamic investigations in both clinical and research environments. This report focuses on the most common urodynamics examinations; uroflowmetry, pressure recording during filling cystometry, and combined pressure-flow studies. The basic aspects of good urodynamic practice are discussed and a strategy for urodynamic measurement, equipment set-up and configuration, signal testing, plausibility controls, pattern recognition, and artifact correction are proposed. The problems of data analysis are mentioned only when they are relevant in the judgment of data quality. In general, recommendations are made for one specific technique. This does not imply that this technique is the only one possible. Rather, it means that this technique is well-established, and gives good results when used with the suggested standards of good urodynamic practice.
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- Spangberg, D., et al.
(author)
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Model extended X-ray absorption fine structure (EXAFS) spectra from molecular dynamics data for Ca2+ and Al3+ aqueous solutions
- 2000
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In: Journal of Physical Chemistry B. - : American Chemical Society (ACS). - 1089-5647 .- 1520-6106 .- 1520-5207. ; 104:45, s. 10467-10472
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Journal article (peer-reviewed)abstract
- Theoretical extended X-ray absorption fine structure (EXAFS) spectra have been computed from molecular dynamics (MD) simulation data. Based on MD-generated molecular geometries, EXAFS spectra were generated with the FEFF6 program, commonly used in the analysis of experimental EXAFS spectra. The effects of multiple scattering and second-shell neighbors on the theoretical spectra were evaluated for CaCl2(aq) and AlCl3(aq), i.e., for two solutions with very different cation hydration structures. The effects are significantly larger for the more structured Al3+ solution, The theoretical Ca2+(aq) spectra are compared with new experimental EXAFS data.
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| 26. |
- Wu, JM, et al.
(author)
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Blue light induced apoptosis in rat retina
- 1999
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In: Eye (London, England). - : Springer Science and Business Media LLC. - 0950-222X .- 1476-5454. ; 1313 ( Pt 4), s. 577-583
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Journal article (peer-reviewed)
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