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- Odell, Adam F, et al.
(author)
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A Novel p53 Mutant Found in Iatrogenic Urothelial Cancers Is Dysfunctional and Can Be Rescued by a Second-site Global Suppressor Mutation
- 2013
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 288:23, s. 16704-16714
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Journal article (peer-reviewed)abstract
- Exposure to herbal remedies containing the carcinogen aristolochic acid (AA) has been widespread in some regions of the world. Rare A→T TP53 mutations were recently discovered in AA-associated urothelial cancers. The near absence of these mutations among all other sequenced human tumors suggests that they could be biologically silent. There are no cell banks with established lines derived from human tumors with which to explore the influence of the novel mutants on p53 function and cellular behavior. To investigate their impact, we generated isogenic mutant clones by integrase-mediated cassette exchange at the p53 locus of platform (null) murine embryonic fibroblasts and kidney epithelial cells. Common tumor mutants (R248W, R273C) were compared with the AA-associated mutants N131Y, R249W, and Q104L. Assays of cell proliferation, migration, growth in soft agar, apoptosis, senescence, and gene expression revealed contrasting outcomes on cellular behavior following introduction of N131Y or Q104L. The N131Y mutant demonstrated a phenotype akin to common tumor mutants, whereas Q104L clone behavior resembled that of cells with wild-type p53. Wild-type p53 responses were restored in double-mutant cells harboring N131Y and N239Y, a second-site rescue mutation, suggesting that pharmaceutical reactivation of p53 function in tumors expressing N131Y could have therapeutic benefit. N131Y is likely to contribute directly to tumor phenotype and is a promising candidate biomarker of AA exposure and disease. Rare mutations thus do not necessarily point to sites where amino acid exchanges are phenotypically neutral. Encounter with mutagenic insults targeting cryptic sites can reveal specific signature hotspots.
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- Talamelli, Alessandro, et al.
(author)
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CICLoPE-a response to the need for high Reynolds number experiments
- 2009
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In: Fluid Dynamics Research. - : IOP Publishing. - 0169-5983 .- 1873-7005. ; 41:2
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Journal article (peer-reviewed)abstract
- Although the equations governing turbulent flow of fluids are well known, understanding the overwhelming richness of flow phenomena, especially in high Reynolds number turbulent flows, remains one of the grand challenges in physics and engineering. High Reynolds number turbulence is ubiquitous in aerospace engineering, ground transportation systems, flow machinery, energy production (from gas turbines to wind and water turbines), as well as in nature, e.g. various processes occurring in the planetary boundary layer. High Reynolds number turbulence is not easily obtained in the laboratory, since in order to have good spatial resolution for measurements, the size of the facility itself has to be large. In this paper, we discuss limitations of various existing facilities and propose a new facility that will allow good spatial resolution even at high Reynolds number. The work is carried out in the framework of the Center for International Cooperation in Long Pipe Experiments (CICLoPE), an international collaboration that many in the turbulence community have shown an interest to participate in.
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