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- Berg, Cecilia, 1976-, et al.
(author)
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Platelet-induced growth of human fibroblasts is associated with an increased expression of 5-lipoxygenase
- 2006
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In: Thrombosis and Haemostasis. - 0340-6245 .- 2567-689X. ; 96:5, s. 652-659
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Journal article (peer-reviewed)abstract
- Proliferation of fibroblasts is vital for adequate wound healing but is probably also involved in different hyperproliferative disorders such as atherosclerosis and cancer. The regeneration of tissue usually starts with coagulation, involving release of mitogenic and inflammatory factors from activated platelets. This study focuses on the role of eicosanoids in the proliferative effects of platelets on human fibroblasts. We show that the phospholipase A2 inhibitor 7,7-dimethyl-5,8-eicosadienoic acid (DMDA), the combined cyclooxygenase (COX) and lipoxygenase (LOX) inhibitor 5,8,11,14-eicosatetraynoic acid (ETYA) and the LOX inhibitor 5,8,11-eicosatriynoic acid (ETI) block the platelet-induced proliferation of serum starved subconfluent human fibroblasts. Anti-proliferative effects were also obtained by specific inhibition of 5-LOX with 5,6-dehydro arachidonic acid (5,6-dAA), whereas the 12-LOX inhibitor cinnamyl-3,4-dihydroxy-α-cyanocinnamate (CDC) did not affect the platelet-stimulated growth of fibroblasts. The expression of 5-LOX was analyzed by reverse-transcriptase-mediated PCR (RT-PCR), Western blotting and HPLC. 5-LOX message and protein was detected in fibroblasts but not in platelets. Incubation with platelets markedly increased, already after one hour, the expression of 5-LOX in the fibroblast culture. The increased 5-LOX activity was associated with an elevated level of the 5-LOX metabolite 5-hydroxyeicosatetraenoic acid (5-HETE) reaching its maximum after 1-2 hours of co-incubation of fibroblasts and platelets. The 5-HETE production was reduced by the inhibitors DMDA, ETYA and ETI. In conclusion, this study suggests that platelet-stimulated proliferation of fibroblasts is mediated by an increased 5-LOX activity, which supports recent findings indicating a crucial role for this enzyme in proliferative disorders such as atherosclerosis. © 2006 Schattauer GmbH, Stuttgart.
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- Sauveroche, Mathilde, et al.
(author)
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Hair cortisol in horses (Equus caballus) in relation to management regimes, personality and breed
- 2020
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In: Journal of Veterinary Behavior. - : Elsevier. - 1558-7878. ; 37
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Journal article (peer-reviewed)abstract
- Hair cortisol is a promising biomarker to measure long-term stress since cortisol is incorporated into the hair shaft as it grows. However, few studies have previously assessed hair cortisol concentrations (HCC) in horses. In this study, HCC was evaluated in both mane hair from the neck and body hair from the withers in 153 horses of different breeds, from seven different stables with three different management regimes (Free-roaming horses, Riding school horses, Trotter horses). In addition, 4 hours of behavioral observations were performed at each stable, and for 43 of the horses, a personality survey was completed. Mane and withers HCC correlated moderately, but significantly (rs=0.48, p<0.001). Differences between the stables were found for both mane and withers hair (both p<0.01) and the stable with lowest HCC also showed highest occurrences of positive social and resting behaviors (both p<0.01). There were no significant differences in HCC between the management regimes even though Free-roaming horses showed less negative social behavior compared to Riding school horses (p=0.041) and Trotter horses (p=0.055). The personality traits Dominance, Anxiousness, and Excitability revealed weak to moderate correlations with mane HCC (rs=-0.34, p=0.027; rs=-0.46, p=0.002; rs=-0.31, p=0.043 respectively) which might suggest that personality could also be related to long-term stress levels in horses.
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