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1.	Beal, Jacob, et al. (author) Robust estimation of bacterial cell count from optical density 2020 In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1 Journal article (peer-reviewed)abstract Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
2.	Liu, Anbu, et al. (author) DDR1/2 enhance KIT activation and imatinib resistance of primary and secondary KIT mutants in gastrointestinal stromal tumors 2024 In: Molecular Carcinogenesis. - 0899-1987. ; 63:1, s. 75-93 Journal article (peer-reviewed)abstract Gastrointestinal stromal tumors (GISTs) are predominantly initiated by KIT mutations. In this study, we observed that discoidin domain receptors 1 and 2 (DDR1 and DDR2) exhibited high expression in GISTs, were associated with KIT, and enhanced the activation of both wild-type KIT and primary KIT mutants. Inhibition of DDR1/2 led to a reduction in the activation of KIT and its downstream signaling molecules, ultimately impairing GIST cell survival and proliferation in vitro. Consequently, treatment of mice carrying germline KIT/V558A mutation with DDR1/2 inhibitor significantly impeded tumor growth, and the combined use of DDR1/2 inhibitor and imatinib, the first-line targeted therapeutic agent for GISTs, markedly enhanced tumor growth suppression. In addition, DDR1/2 inhibition resulted in decreased KIT expression, while KIT inhibition led to upregulation of DDR1/2 expression in GISTs. The presence of DDR1/2 also decreased the sensitivity of wild-type KIT or primary KIT mutants to imatinib, indicating a possible role for DDR1/2 in promoting GIST survival during KIT-targeted therapy. The development of drug-resistant secondary KIT mutations is a primary factor contributing to GIST recurrence following targeted therapy. Similar to primary KIT mutants, DDR1/2 can associate with and enhance the activation of secondary KIT mutants, further diminishing their sensitivity to imatinib. In summary, our data demonstrate that DDR1/2 contribute to KIT activation in GISTs and strengthen resistance to imatinib for both primary and secondary KIT mutants, providing a rationale for further exploration of DDR1/2 targeting in GIST treatment.
3.	Xu, Peng, et al. (author) D-A-D-Typed Hole Transport Materials for Efficient Perovskite Solar Cells : Tuning Photovoltaic Properties via the Acceptor Group 2018 In: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 10:23, s. 19697-19703 Journal article (peer-reviewed)abstract Two D-A-D-structured hole-transport materials (YN1 and YN2) have been synthesized and used in perovskite solar cells. The two HTMs have low-lying HOMO levels and impressive mobility. Perovskite-based solar cells (PSCs) fabricated with YN2 showed a power conversion efficiency (PCE) value of 19.27% in ambient air, which is significantly higher than that of Spiro-OMeTAD (17.80%). PSCs based on YN1 showed an inferior PCE of 16.03%. We found that the incorporation of the stronger electron-withdrawing group in the HTM YN2 improves the PCE of PSCs. Furthermore, the YN2-based PSCs exhibit good long-term stability retaining 91.3% of its initial efficiency, whereas PSCs based on Spiro-OMeTAD retained only 42.2% after 1000 h lifetime (dark conditions). These promising results can provide a new strategy for the design of D-A-D HTMs for PSC applications in future.
4.	Yang, Yang, et al. (author) NDDVD : an integrated and manually curated Neurodegenerative Diseases Variation Database 2018 In: Database: the journal of biological databases and curation. - : Oxford University Press (OUP). - 1758-0463. ; 2018 Journal article (peer-reviewed)abstract Neurodegenerative diseases (NDDs) are associated with genetic variations including point substitutions, copy number alterations, insertions and deletions. At present, a few genetic variation repositories for some individual NDDs have been created, however, these databases are needed to be integrated and expanded to all the NDDs for systems biological investigation. We here build a relational database termed as NDDVD to integrate all the variations of NDDs using Leiden Open Variation Database (LOVD) platform. The items in the NDDVD are collected manually from PubMed or extracted from the existed variation databases. The cross-disease database includes over 6374 genetic variations of 289 genes associated with 37 different NDDs. The patterns, conservations and biological functions for variations in different NDDs are statistically compared and a user-friendly interface is provided for NDDVD at: http://bioinf.suda.edu.cn/NDDvarbase/LOVDv.3.0.
5.	Bao, Zhigang, et al. (author) Central Limit Theorem for Mesoscopic Eigenvalue Statistics of the Free Sum of Matrices 2020 In: International mathematics research notices. - : Oxford University Press (OUP). - 1073-7928 .- 1687-0247. ; 2022:7, s. 5320-5382 Journal article (peer-reviewed)abstract We consider random matrices of the form H-N = A(N) + UNBNUN*, where A(N) and B-N are two N by N deterministic Hermitian matrices and U-N is a Haar distributed random unitary matrix. We establish a universal central limit theorem for the linear eigenvalue statistics of H-N on all mesoscopic scales inside the regular bulk of the spectrum. The proof is based on studying the characteristic function of the linear eigenvalue statistics and consists of two main steps: (1) generating Ward identities using the left-translation invariance of the Haar measure, along with a local law for the resolvent of H-N and analytic subordination properties of the free additive convolution, allows us to derive an explicit formula for the derivative of the characteristic function; (2) a local law for two-point product functions of resolvents is derived using a partial randomness decomposition of the Haar measure. We also prove the corresponding results for orthogonal conjugations.
6.	Cheng, Peirui, et al. (author) Highly Efficient Ruddlesden–Popper Halide Perovskite PA2MA4Pb5I16 Solar Cells 2018 In: ACS Energy Letters. - : American Chemical Society (ACS). - 2380-8195. ; 3:8, s. 1975-1982 Journal article (peer-reviewed)abstract Two-dimensional (2D) Ruddlesden-Popper (RP) organic-inorganic perovskites have emerged as promising candidates for solar cells with technologically relevant stability. Herein, a new RP perovskite, the fifth member («n» = 5) of the (CH3(CH2)2NH3)2(CH3NH3)n-1PbnI3n+1 family (abbreviated as PA2MA4Pb5I16), was synthesized and systematically investigated in terms of photovoltaic application. The obtained pure PA2MA4Pb5I16 crystal exhibits a direct band gap of Eg = 1.85 eV. Systematic analysis on the solid film highlights the key role of the precursor-solvent interaction in the quantum well orientation, phase purity, grain size, surface quality, and optoelectronic properties, which can be well-tuned with addition of dimethyl sulfoxide (DMSO) into the N,N-dimethylformamide (DMF) precursor solution. These findings present opportunities for designing a high-quality RP film with well-controlled quantum well orientation, micrometer-sized grains, and optoelectronic properties. As a result, we achieved power conversion efficiency (PCE) up to 10.41%.
7.	Du, Wanyi, et al. (author) Photodoping of graphene/silicon van der Waals heterostructure observed by terahertz emission spectroscopy 2020 In: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 117:8 Journal article (peer-reviewed)abstract Photodoping as a nonvolatile and reversible method can be used to modify the carrier distribution at the heterojunction interface. Herein, we explore the 2D/3D van der Waals (vdW) graphene/silicon (G/Si) heterostructure in real time by terahertz (THz) emission spectroscopy. Photoinduced doping is introduced by a continuous wave laser, which leads to a screening effect to the built-in electric field at the interface. The resulting decrease in transient photocurrent reduces the THz emission amplitude from the G/Si heterostructure. The photoinduced doping effect suggests a 40% THz intrinsic modulation depth at external reverse bias. This work provides an effective way to actively control the THz emission process from the G/Si interface and paves the way for analyzing the interfacial process under photoinduced doping in vdW heterostructures.
8.	Eichhorn, S. J., et al. (author) Current international research into cellulose as a functional nanomaterial for advanced applications 2022 In: Journal of Materials Science. - : Springer Nature. - 0022-2461 .- 1573-4803. ; 57:10, s. 5697-5767 Journal article (peer-reviewed)abstract This review paper provides a recent overview of current international research that is being conducted into the functional properties of cellulose as a nanomaterial. A particular emphasis is placed on fundamental and applied research that is being undertaken to generate applications, which are now becoming a real prospect given the developments in the field over the last 20 years. A short introduction covers the context of the work, and definitions of the different forms of cellulose nanomaterials (CNMs) that are most widely studied. We also address the terminology used for CNMs, suggesting a standard way to classify these materials. The reviews are separated out into theme areas, namely healthcare, water purification, biocomposites, and energy. Each section contains a short review of the field within the theme and summarizes recent work being undertaken by the groups represented. Topics that are covered include cellulose nanocrystals for directed growth of tissues, bacterial cellulose in healthcare, nanocellulose for drug delivery, nanocellulose for water purification, nanocellulose for thermoplastic composites, nanocellulose for structurally colored materials, transparent wood biocomposites, supercapacitors and batteries.
9.	Holden, Stein T., et al. (author) Tenure Security and Investment Effects of Forest Tenure Reform in China 2013 In: Land Tenure Reform in Asia and Africa: assessing impacts on poverty and natural resource management / edited by Stein T. Holden, Keijiro Otsuka and Klaus Deininger... - UK : Palgrave Macmillan. - 9781137343802 ; , s. 256-282 Book chapter (peer-reviewed)abstract http://www.palgraveconnect.com/pc/doifinder/10.1057/9781137343819.0020
10.	Hu, Xiangzhao, et al. (author) Boosting Industrial-Level CO2 Electroreduction of N-Doped Carbon Nanofibers with Confined Tin-Nitrogen Active Sites via Accelerating Proton Transport Kinetics 2023 In: Advanced Functional Materials. - : John Wiley and Sons Inc. - 1616-301X .- 1616-3028. ; 33:4 Journal article (peer-reviewed)abstract The development of highly efficient robust electrocatalysts with low overpotential and industrial-level current density is of great significance for CO2 electroreduction (CO2ER), however the low proton transport rate during the CO2ER remains a challenge. Herein, a porous N-doped carbon nanofiber confined with tin-nitrogen sites (Sn/NCNFs) catalyst is developed, which is prepared through an integrated electrospinning and pyrolysis strategy. The optimized Sn/NCNFs catalyst exhibits an outstanding CO2ER activity with the maximum CO FE of 96.5%, low onset potential of −0.3 V, and small Tafel slope of 68.8 mV dec−1. In a flow cell, an industrial-level CO partial current density of 100.6 mA cm−2 is achieved. In situ spectroscopic analysis unveil the isolated Sn-N site acted as active center for accelerating water dissociation and subsequent proton transport process, thus promoting the formation of intermediate COOH in the rate-determining step for CO2ER. Theoretical calculations validate pyrrolic N atom adjacent to the Sn-N active species assisted reducing the energy barrier for COOH formation, thus boosting the CO2ER kinetics. A Zn-CO2 battery is designed with the cathode of Sn/NCNFs, which delivers a maximum power density of 1.38 mW cm−2 and long-term stability.
11.	Huang, Kun, et al. (author) Enhanced peak growth of global vegetation and its key mechanisms 2018 In: Nature Ecology and Evolution. - : Springer Science and Business Media LLC. - 2397-334X. ; 2:12, s. 1897-1905 Journal article (peer-reviewed)abstract The annual peak growth of vegetation is critical in characterizing the capacity of terrestrial ecosystem productivity and shaping the seasonality of atmospheric CO2 concentrations. The recent greening of global lands suggests an increasing trend of terrestrial vegetation growth, but whether or not the peak growth has been globally enhanced still remains unclear. Here, we use two global datasets of gross primary productivity (GPP) and a satellite-derived Normalized Difference Vegetation Index (NDVI) to characterize recent changes in annual peak vegetation growth (that is, GPPmax and NDVImax). We demonstrate that the peak in the growth of global vegetation has been linearly increasing during the past three decades. About 65% of the NDVImax variation is evenly explained by expanding croplands (21%), rising CO2 (22%) and intensifying nitrogen deposition (22%). The contribution of expanding croplands to the peak growth trend is substantiated by measurements from eddy-flux towers, sun-induced chlorophyll fluorescence and a global database of plant traits, all of which demonstrate that croplands have a higher photosynthetic capacity than other vegetation types. The large contribution of CO2 is also supported by a meta-analysis of 466 manipulative experiments and 15 terrestrial biosphere models. Furthermore, we show that the contribution of GPPmax to the change in annual GPP is less in the tropics than in other regions. These multiple lines of evidence reveal an increasing trend in the peak growth of global vegetation. The findings highlight the important roles of agricultural intensification and atmospheric changes in reshaping the seasonality of global vegetation growth.
12.	Huang, Yuanyuan, et al. (author) Hidden spin polarization in the centrosymmetric Mo S2 crystal revealed via elliptically polarized terahertz emission 2020 In: Physical Review B. - 2469-9950. ; 102:8 Journal article (peer-reviewed)abstract The spin polarization in centrosymmetric molybdenum disulfide (MoS2) bulk crystal is recorded by helicity dependent photocurrent exposed as elliptically polarized terahertz (THz) emission. We assign this observation to hidden spin polarization due to the local Dresselhaus effect induced by the molybdenum atomic site asymmetry. This spin polarization induces ultrafast optically controlled circular photogalvanic current that results in elliptically polarized THz emission. Furthermore, this THz radiation exhibits a helicity dependent bleaching effect under opposite circularly polarized excitations due to the spin relaxation. Our results demonstrate the viability of all-optical control of hidden spin polarization in two-dimensional materials and propose the applicability of the THz emission spectroscopy as a sensitive and contactless method to study spintronic physics in two-dimensional materials.
13.	Kolahdouz, Mohammadreza, et al. (author) Carbon-Related Materials : Graphene and Carbon Nanotubes in Semiconductor Applications and Design 2022 In: Micromachines. - : MDPI AG. - 2072-666X. ; 13:8 Research review (peer-reviewed)abstract As the scaling technology in the silicon-based semiconductor industry is approaching physical limits, it is necessary to search for proper materials to be utilized as alternatives for nanoscale devices and technologies. On the other hand, carbon-related nanomaterials have attracted so much attention from a vast variety of research and industry groups due to the outstanding electrical, optical, mechanical and thermal characteristics. Such materials have been used in a variety of devices in microelectronics. In particular, graphene and carbon nanotubes are extraordinarily favorable substances in the literature. Hence, investigation of carbon-related nanomaterials and nanostructures in different ranges of applications in science, technology and engineering is mandatory. This paper reviews the basics, advantages, drawbacks and investigates the recent progress and advances of such materials in micro and nanoelectronics, optoelectronics and biotechnology.
14.	Lensink, Marc F., et al. (author) Impact of AlphaFold on structure prediction of protein complexes: The CASP15-CAPRI experiment 2023 In: Proteins. - : WILEY. - 0887-3585 .- 1097-0134. Journal article (peer-reviewed)abstract We present the results for CAPRI Round 54, the 5th joint CASP-CAPRI protein assembly prediction challenge. The Round offered 37 targets, including 14 homodimers, 3 homo-trimers, 13 heterodimers including 3 antibody-antigen complexes, and 7 large assemblies. On average similar to 70 CASP and CAPRI predictor groups, including more than 20 automatics servers, submitted models for each target. A total of 21 941 models submitted by these groups and by 15 CAPRI scorer groups were evaluated using the CAPRI model quality measures and the DockQ score consolidating these measures. The prediction performance was quantified by a weighted score based on the number of models of acceptable quality or higher submitted by each group among their five best models. Results show substantial progress achieved across a significant fraction of the 60+ participating groups. High-quality models were produced for about 40% of the targets compared to 8% two years earlier. This remarkable improvement is due to the wide use of the AlphaFold2 and AlphaFold2-Multimer software and the confidence metrics they provide. Notably, expanded sampling of candidate solutions by manipulating these deep learning inference engines, enriching multiple sequence alignments, or integration of advanced modeling tools, enabled top performing groups to exceed the performance of a standard AlphaFold2-Multimer version used as a yard stick. This notwithstanding, performance remained poor for complexes with antibodies and nanobodies, where evolutionary relationships between the binding partners are lacking, and for complexes featuring conformational flexibility, clearly indicating that the prediction of protein complexes remains a challenging problem.
15.	Li, Dianxiang, et al. (author) Expression of the Shrimp wap gene in Drosophila elicits defense responses and protease inhibitory activity 2018 In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 8 Journal article (peer-reviewed)abstract The wap gene encodes a single whey acidic protein (WAP) domain-containing peptide from Chinese white shrimp (Fenneropenaeus chinensis), which shows broad-spectrum antimicrobial activities and proteinase inhibitory activities in vitro. To explore the medical applications of the WAP peptide, a wap gene transgenic Drosophila melanogaster was constructed. In wap-expressing flies, high expression levels of wap gene (> 100 times) were achieved, in contrast to those of control flies, by qRT-PCR analysis. The wap gene expression was associated with increased resistance to microbial infection and decreased bacterial numbers in the flies. In addition, the WAP protein extract from wap-expressing flies, compared with control protein extract from control flies, showed improved antimicrobial activities against broad Gram-positive and Gram-negative bacteria, including the clinical drug resistant bacterium of methicillin-resistant S. aureus (MRSA), improved protease inhibitor activities against crude proteinases and commercial proteinases, including elastase, subtilis proteinase A, and proteinase K in vitro, and improved growth rate and microbial resistance, as well as wound-healing in loach and mouse models. These results suggest that wap-expressing flies could be used as a food additive in aquaculture to prevent infections and a potential antibacterial for fighting drug-resistant bacteria.
16.	Li, Yiting, et al. (author) Central limit theorem for mesoscopic eigenvalue statistics of deformed Wigner matrices and sample covariance matrices 2021 In: Annales de l'I.H.P. Probabilites et statistiques. - : Project Euclid. - 0246-0203 .- 1778-7017. ; 57:1, s. 506-546 Journal article (peer-reviewed)abstract We consider N by N deformed Wigner random matrices of the form X-N = H-N + A(N), where H-N is a real symmetric or complex Hermitian Wigner matrix and A(N) is a deterministic real bounded diagonal matrix. We prove a universal Central Limit Theorem for the linear eigenvalue statistics of X-N for all mesoscopic scales both in the spectral bulk and at regular edges where the global eigenvalue density vanishes as a square root. The method relies on studying the characteristic function of the linear statistics (Landon and Sosoe (2018)) by using the cumulant expansion method, along with local laws for the Green function of X-N (Ann. Probab. 48 (2020) 963-1001; Probab. Theory Related Fields 169 (2017) 257-352; J. Math. Phys. 54 (2013) 103504) and analytic subordination properties of the free additive convolution (Dallaporta and Fevrier (2019); Random Matrices Theory Appl. 9 (2020) 2050011). We also prove the analogous results for high-dimensional sample covariance matrices.
17.	Li, Yiting, et al. (author) On fluctuations of global and mesoscopic linear statistics of generalized Wigner matrices 2021 In: Bernoulli. - : Bernoulli Society for Mathematical Statistics and Probability. - 1350-7265 .- 1573-9759. ; 27:2, s. 1057-1076 Journal article (peer-reviewed)abstract We consider an N by N real or complex generalized Wigner matrix H-N, whose entries are independent centered random variables with uniformly bounded moments. We assume that the variance profile, s(ij) := E vertical bar H-ij vertical bar(2), satisfies Sigma(N)(i=1) s(ij) = 1, for all 1 <= j <= N and c(-1) <= Ns(ij) <= c for all 1 <= i, j <= N with some constant c >= 1. We establish Gaussian fluctuations for the linear eigenvalue statistics of HN on global scales, as well as on all mesoscopic scales up to the spectral edges, with the expectation and variance formulated in terms of the variance profile. We subsequently obtain the universal mesoscopic central limit theorems for the linear eigenvalue statistics inside the bulk and at the edges, respectively.
18.	Li, Yuanyuan, et al. (author) Optically Transparent Wood Substrate for Perovskite Solar Cells 2019 In: ACS Sustainable Chemistry and Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 7:6, s. 6061-6067 Journal article (peer-reviewed)abstract Transparent wood is a candidate for use as an energy-saving building material due to its low density (ca. 1.2 g/cm(3)), high optical transmittance (over 85% at 1 mm thickness), low thermal conductivity (0.23 W m(-1) K-1), and good load-bearing performance with tough failure behavior (no shattering). High optical transmittance also makes transparent wood a candidate for optoelectronic devices. In this work, for the first time, perovskite solar cells processed at low temperature (<150 degrees C) were successfully assembled directly on transparent wood substrates. A power conversion efficiency up to 16.8% was obtained. The technologies demonstrated may pave the way for integration of solar cells with light transmitting wood building structures for energy-saving purposes.
19.	Liu, Peng, et al. (author) Novel and Stable D-A-π-A Dyes for Efficient Solid-state Dye-sensitized Solar Cells 2017 In: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 2:5, s. 1812-1819 Journal article (peer-reviewed)abstract Two novel organic donor–acceptor−π–acceptor sensitizers, W7 and W8, have been applied in efficient solid-state dye-sensitized solar cells (ssDSSCs). Using 2,2′,7,7′-tetrakis(N,N-di-p-methoxyphenyl-amine) 9,9′-spirobifluorene (Spiro-OMeTAD) as hole-transport material (HTM), an excellent power conversion efficiency of 6.9% was recorded for W7, together with an excellent photocurrent of 10.51 mA cm–2 and a high open-circuit voltage of 880 mV under standard AM 1.5 G illumination (100 mW cm–2). The solid-state solar cells based on W8 showed an efficiency of 5.2%, with a good photocurrent of 9.55 mA cm–2 and an open-circuit voltage of 870 mV. Compared to that of the well-known WS2 sensitizer, the results show that the performance of the ssDSSC devices can be significantly improved by introducing triphenylamine moiety into their structure. In addition, results of photoinduced absorption spectroscopy show efficient dye regeneration for W7- and W8-based devices. A higher hole conductivity of the W7/HTM and W8/HTM layers compared to that of the WS2/HTM layer was observed, indicating an efficient charge transfer at the interfaces. The results obtained offer insights into the design of reliable and highly efficient ssDSSCs for large-scale applications.
20.	Liu, Yi, et al. (author) Porous, robust, thermally stable, and flame retardant nanocellulose/polyimide separators for safe lithium-ion batteries 2023 In: Journal of Materials Chemistry A. - : Royal Society of Chemistry (RSC). - 2050-7488 .- 2050-7496. ; 11:43, s. 23360-23369 Journal article (peer-reviewed)abstract The safety of lithium-ion batteries (LIBs) is paramount for all users. One effective way to improve safety is incorporating heat-resistant polyimide (PI) separators, which can increase the thermal stability of batteries and minimize the risk of thermal runaway. However, preparing PI separators with both an ideal pore structure and adequate mechanical properties remains as a challenge. Here, we introduced decabromodiphenyl ethane (DBDPE) and cellulose nanofibers (CNFs) into PI and produced a hybrid separator with an outstanding pore structure and excellent mechanical properties. Aided with DBDPE, the separators attain a well-defined and uniform pore size (20 nm), while demonstrating high porosities (78%) through phase inversion processes. Owing to the addition of CNFs, the mechanical properties of the separators were significantly improved, with a tensile strength of 25.4 MPa and an elastic modulus of 550.1 MPa. Moreover, the separators demonstrate high ion conductivity (0.45 mS cm-1), excellent thermal-dimensional stability (up to 200 degrees C), remarkable flame retardancy, and outstanding electrolyte wettability. At room temperature, the batteries with the separators demonstrate comparable performance with those of polypropylene (PP) separators. However, when subjected to thermal shock treatments, the batteries with the separators outperform those with PP, showcasing their superior performance. The work introduces a novel strategy for designing high-performance separators, thereby paving the way for advancements in the fabrication of LIBs with enhanced safety features. A porous, robust, and thermally stable hybrid separator was developed to solve the dilemma between desired pore structures and mechanical properties in polyimide separators by introducing decabromodiphenyl ethane and cellulose nanofibers.
21.	Martínez Barrio, Álvaro, et al. (author) The genetic basis for ecological adaptation of the Atlantic herring revealed by genome sequencing 2016 In: eLIFE. - 2050-084X. ; 5 Journal article (peer-reviewed)abstract Ecological adaptation is of major relevance to speciation and sustainable population management, but the underlying genetic factors are typically hard to study in natural populations due to genetic differentiation caused by natural selection being confounded with genetic drift in subdivided populations. Here, we use whole genome population sequencing of Atlantic and Baltic herring to reveal the underlying genetic architecture at an unprecedented detailed resolution for both adaptation to a new niche environment and timing of reproduction. We identify almost 500 independent loci associated with a recent niche expansion from marine (Atlantic Ocean) to brackish waters (Baltic Sea), and more than 100 independent loci showing genetic differentiation between spring- and autumn-spawning populations irrespective of geographic origin. Our results show that both coding and non-coding changes contribute to adaptation. Haplotype blocks, often spanning multiple genes and maintained by selection, are associated with genetic differentiation.
22.	Pan, Xiong-Fei, et al. (author) Circulating fatty acids and risk of gestational diabetes mellitus : prospective analyses in China 2021 In: European Journal of Endocrinology. - : Bioscientifica. - 0804-4643 .- 1479-683X. ; 185:1, s. 87-97 Journal article (peer-reviewed)abstract Objective: We aimed to examine prospective associations between circulating fatty acids in early pregnancy and incident gestational diabetes mellitus (GDM) among Chinese pregnant women.Methods: Analyses were based on two prospective nested case-control studies conducted in western China (336 GDM cases and 672 matched controls) and central China (305 cases and 305 matched controls). Fasting plasma fatty acids in early pregnancy (gestational age at enrollment: 10.4 weeks(s.d., 2.0)) and 13.2 weeks (1.0), respectively) were determined by gas chromatography-mass spectrometry, and GDM was diagnosed based on the International Association of Diabetes in Pregnancy Study Groups criteria during 24-28 weeks of gestation. Multiple metabolic biomarkers (HOMA-IR (homeostatic model assessment for insulin resistance), HbA1c, c-peptide, high-sensitivity C-reactive protein, adiponectin, leptin, and blood lipids) were additionally measured among 672 non-GDM controls at enrollment.Results: Higher levels of saturated fatty acids (SFAs) 14:0 (pooled odds ratio, 1.41 for each 1-s.d. increase; 95% CI: 1.25, 1.59) and 16:0 (1.19; 1.05, 1.35) were associated with higher odds of GDM. Higher levels of n-6 polyunsaturated fatty acid (PUFA) 18:2n-6 were strongly associated with lower odds of GDM (0.69; 0.60, 0.80). In non-GDM pregnant women, higher SFAs 14:0 and 16:0 but lower n-6 PUFA 18:2n-6 were generally correlated with unfavorable metabolic profiles.Conclusions: We documented adverse associations of 14:0 and 16:0 but a protective association of 18:2n-6 with GDM among Chinese pregnant women. Our findings highlight the distinct roles of specific fatty acids in the onset of GDM.
23.	Pan, Yitao, et al. (author) Novel Chlorinated Polyfluorinated Ether Sulfonates and Legacy Per-/Polyfluoroalkyl Substances : Placental Transfer and Relationship with Serum Albumin and Glomerular Filtration Rate 2017 In: Environmental Science and Technology. - Dordrecht, Neteherlands : Springer Netherlands. - 0013-936X .- 1520-5851. ; 51:1, s. 634-644 Journal article (peer-reviewed)abstract Per- and polyfluoroalkyl substances (PFASs) may cross the placental barrier and lead to fetal exposure. However, little is known about the factors that influence maternal-fetal transfer of these chemicals. PFAS concentrations were analyzed in 100 paired samples of human maternal sera collected in each trimester and cord sera at delivery; these samples were collected in Wuhan, China, 2014. Linear regression was used to estimate associations of transfer efficiencies with factors. Chlorinated polyfluorinated ether sulfonates (Cl-PFAESs, 6:2 and 8:2) were frequently detected (>99%) in maternal and cord sera. A significant decline in PFAS levels during the three trimesters was observed. A U-shape trend for transfer efficiency with increasing chain length was observed for both carboxylates and sulfonates. Higher transfer efficiencies of PFASs were associated with advancing maternal age, higher education, and lower glomerular filtration rate (GFR). Cord serum albumin was a positive factors for higher transfer efficiency (increased 1.1-4.1% per 1g/L albumin), whereas maternal serum albumin tended to reduce transfer efficiency (decreased 2.4-4.3% per 1g/L albumin). Our results suggest that exposure to Cl-PFAESs may be widespread in China. The transfer efficiencies among different PFASs were structure-dependent. Physiological factors (e.g., GFR and serum albumin) were observed for the first time to play critical roles in PFAS placental transfer.
24.	Schnelli, Kevin, et al. (author) Convergence rate to the Tracy–Widom laws for the largest eigenvalue of sample covariance matrices 2023 In: The Annals of Applied Probability. - : Institute of Mathematical Statistics. - 1050-5164 .- 2168-8737. ; 33:1, s. 677-725 Journal article (peer-reviewed)abstract We establish a quantitative version of the Tracy–Widom law for the largest eigenvalue of high-dimensional sample covariance matrices. To be precise, we show that the fluctuations of the largest eigenvalue of a sample covariance matrix X∗X converge to its Tracy–Widom limit at a rate nearly N-1/3, where X is an M × N random matrix whose entries are independent real or complex random variables, assuming that both M and N tend to infinity at a constant rate. This result improves the previous estimate N-2/9 obtained by Wang (2019). Our proof relies on a Green function comparison method (Adv. Math. 229 (2012) 1435–1515) using iterative cumulant expansions, the local laws for the Green function and asymptotic properties of the correlation kernel of the white Wishart ensemble.
25.	Schnelli, Kevin, et al. (author) Quantitative Tracy–Widom laws for the largest eigenvalue of generalized Wigner matrices 2023 In: Electronic Journal of Probability. - : Institute of Mathematical Statistics. - 1083-6489. ; 28 Journal article (peer-reviewed)abstract We show that the fluctuations of the largest eigenvalue of any generalized Wigner matrix H converge to the Tracy–Widom laws at a rate nearly O(N−1/3), as the matrix dimension N tends to infinity. We allow the variances of the entries of H to have distinct values but of comparable sizes such that (formula presented). Our result improves the previous rate O(N−2/9) by Bourgade [8] and the proof relies on the first long-time Green function comparison theorem near the edges without the second moment matching restriction.
26.	Sher, Omer, et al. (author) Analysis of molecular ligand functionalization process in nano-molecular electronic devices containing densely packed nano-particle functionalization shells 2022 In: Nanotechnology. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 33:25 Journal article (peer-reviewed)abstract Molecular electronic devices based on few and single-molecules have the advantage that the electronic signature of the device is directly dependent on the electronic structure of the molecules as well as of the electrode-molecule junction. In this work, we use a two-step approach to synthesise functionalized nanomolecular electronic devices (nanoMoED). In first step we apply an organic solvent-based gold nanoparticle (AuNP) synthesis method to form either a 1-dodecanethiol or a mixed 1-dodecanethiol/omega-tetraphenyl ether substituted 1-dodecanethiol ligand shell. The functionalization of these AuNPs is tuned in a second step by a ligand functionalization process where biphenyldithiol (BPDT) molecules are introduced as bridging ligands into the shell of the AuNPs. From subsequent structural analysis and electrical measurements, we could observe a successful molecular functionalization in nanoMoED devices as well as we could deduce that differences in electrical properties between two different device types are related to the differences in the molecular functionalization process for the two different AuNPs synthesized in first step. The same devices yielded successful NO2 gas sensing. This opens the pathway for a simplified synthesis/fabrication of molecular electronic devices with application potential.
27.	Soshnikov, A., et al. (author) Gaussian approximation of the distribution of strongly repelling particles on the unit circle 2021 In: Theory of Probability and its Applications. - : Society for Industrial & Applied Mathematics (SIAM). - 0040-585X .- 1095-7219. ; 65:4, s. 588-615 Journal article (peer-reviewed)abstract In this paper, we consider a strongly repelling model of n ordered particles {e(i theta j)}(j=0)(n-1 )with the density p(theta(0), ..., theta(n-1)) = 1/Z(n )exp { - beta/2 Sigma(j not equal k )sin(-2 )(theta(j) - theta(k)/2)}, beta > 0. Let theta(j )= 2 pi j/n + x(j)/n(2) + const such that Sigma(n-1 )(j=0)x(j )= 0. Define zeta(n) (2 pi j/n) = x(j)/root n, and extend zeta(n) piecewise linearly to [0, 2 pi]. We prove the functional convergence of zeta(n)(t) to zeta(t) = root 2/beta Re (Sigma(infinity )(k=1)1/k e(ikt) Z(k)), where Z(k ) are independent identically distributed complex standard Gaussian random variables.
28.	Wang, JunXin, et al. (author) Extraction of Backscattering and Absorption Coefficients of Magnetite Nanosphere Composites from Light-Scattering Measurements : Implications for Optomagnetic Sensing 2020 In: ACS Applied Nano Materials. - : American Chemical Society (ACS). - 2574-0970. ; 3, s. 11172-11183 Journal article (peer-reviewed)abstract Interaction of light with magnetic nanoparticles, dispersed insolution or embedded in other materials, is of major interest in a range of applications, one example being optomagnetic sensors. In applied research, light absorption and scattering of nanoparticle composites are often quantified by the Kubelka−Munk two-flux radiative transfer model. In this paper, we synthesized magnetite (Fe3O4) nanospheres with different diameters and encapsulated them into a polymer matrix. Their spectral transmittance and reflectance were investigated by spectrophotometry, together with measurements of angle-resolved scattering in the forward and backward hemispheres. The measured angular distribution was applied to approximate the scattering-phase function inside the film, which could be well described by the revised Reynolds−McCormick model. The backscattering and absorption coefficients were derived by inversion of the Kubelka−Munk relations, using the interface reflectances obtained from angle-dependent measurements. We present detailed optical properties for samples with various particle concentrations and scattering layer thicknesses, consisting of the magnetite and polymer composites. The absorption and backscattering coefficients for particles of diameter 458 nm showed qualitative agreement with single-scattering Mie calculations. The optical properties of composites with smaller particles might beinfluenced by an oxidized Fe2O3-like surface layer. The present approach can be used to study different kinds of magnetic nanoparticle clusters, dispersed in a supporting medium, and thus provide optical parameters of relevance for interpreting results ofoptomagnetic sensing experiments.
29.	Wang, Linqin, et al. (author) A crosslinked polymer as dopant-free hole-transport material for efficient n-i-p type perovskite solar cells 2021 In: Journal of Energy Chemistry. - : Elsevier BV. - 2095-4956 .- 2096-885X. ; 55, s. 211-218 Journal article (peer-reviewed)abstract A new crosslinked polymer, called P65, with appropriate photo-electrochemical, opto-electronic, and thermal properties, has been designed and synthesized as an efficient, dopant-free, hole-transport material (HTM) for n-i-p type planar perovskite solar cells (PSCs). P65 is obtained from a low-cost and easily synthesized spiro[fluorene-9,9′-xanthene]-3′,6′-diol (SFX-OH)-based monomer X65 through a free-radical polymerization reaction. The combination of a three-dimensional (3D) SFX core unit, hole-transport methoxydiphenylamine group, and crosslinked polyvinyl network provides P65 with good solubility and excellent film-forming properties. By employing P65 as a dopant-free hole-transport layer in conventional n-i-p type PSCs, a power conversion efficiency (PCE) of up to 17.7% is achieved. To the best of our knowledge, this is the first time a 3D, crosslinked, polymeric dopant-free HTM has been reported for use in conventional n-i-p type PSCs. This study provides a new strategy for the future development of a 3D crosslinked polymeric dopant-free HTM with a simple synthetic route and low-cost for commercial, large-scale applications in future PSCs.
30.	Wang, Linqin, et al. (author) Design and synthesis of dopant-free organic hole-transport materials for perovskite solar cells 2018 In: Chemical Communications. - : ROYAL SOC CHEMISTRY. - 1359-7345 .- 1364-548X. ; 54:69, s. 9571-9574 Journal article (peer-reviewed)abstract Two novel dopant-free hole-transport materials (HTMs) with spiro[dibenzo[c,h]xanthene-7,9-fluorene] (SDBXF) skeletons were prepared via facile synthesis routes. A power conversion efficiency of 15.9% in perovskite solar cells is attained by using one HTM without dopants, which is much higher than undoped Spiro-OMeTAD-based devices (10.8%). The crystal structures of both new HTMs were systematically investigated to reveal the reasons behind such differences in performance and to indicate the design principles of more advanced HTMs.
31.	Wang, Linqin, et al. (author) Impact of Linking Topology on the Properties of Carbazole-Based Hole-Transport Materials and their Application in Solid-State Mesoscopic Solar Cells 2019 In: Solar RRL. - : Wiley-VCH Verlagsgesellschaft. - 2367-198X. ; 3:9 Journal article (peer-reviewed)abstract Carbazole is a promising core for the molecular design of hole-transport materials (HTMs) for solid-state mesoscopic solar cells (ssMSCs), such as solid-state dye-sensitized solar cells (ssDSSCs) and perovskite solar cells (PSCs) due to its low cost and excellent optoelectronic properties of its derivatives. Although carbazole-based HTMs are intensely investigated in ssMSCs and promising device performance is demonstrated, the fundamental understanding of the impact of linking topology on the properties of carbazole-based HTMs is lacking. Herein, the effect of the linking topology on the optical and electronic properties of a series of carbazole-based HTMs with 2,7-substitution and 3,6-substitution is systematically investigated. The results demonstrate that the 2,7-substituted carbazole-based HTMs display higher hole mobility and conductivity among this series of analogous molecules, thereby exhibiting better device performance. In addition, the conductivity of the HTMs is improved after light treatment, which explains the commonly observed light-soaking phenomenon of ssMSCs in general. All these carbazole-based HTMs are successfully applied in ssMSCs and one of the HTMs X50-based devices yield a promising efficiency of 6.8% and 19.2% in ssDSSCs and PSCs, respectively. This study provides guidance for the molecular design of effective carbazole-based HTMs for high-performance ssMSCs and related electronic devices.
32.	Xu, Bo, et al. (author) Tailor-Making Low-Cost Spiro[fluorene-9,9′-xanthene]-Based 3D Oligomers for Perovskite Solar Cells 2017 In: Chem. - : Elsevier. - 2451-9308 .- 2451-9294. ; 2:5, s. 676-687 Journal article (peer-reviewed)abstract The power-conversion efficiencies (PCEs) of perovskite solar cells (PSCs) have increased rapidly from about 4% to 22% during the past few years. One of the major challenges for further improvement of the efficiency of PSCs is the lack of sufficiently good hole transport materials (HTMs) to efficiently scavenge the photogenerated holes and aid the transport of the holes to the counter-electrode in the PSCs. In this study, we tailor-made two low-cost spiro[fluorene-9,9′-xanthene] (SFX)-based 3D oligomers, termed X54 and X55, by using a one-pot synthesis approach for PSCs. One of the HTMs, X55, gives a much deeper HOMO level and a higher hole mobility and conductivity than the state-of-the-art HTM, Spiro-OMeTAD. PSC devices based on X55 as the HTM show a very impressive PCE of 20.8% under 100 mW·cm−2 AM1.5G solar illumination, which is much higher than the PCE of the reference devices based on Spiro-OMeTAD (18.8%) and X54 (13.6%) under the same conditions.
33.	Xu, Jingxiang, et al. (author) Ulva lactuca polysaccharide inhibits hepatocellular carcinoma growth by induces the expression of CD5L and activates complement cascade 2024 In: Journal of Functional Foods. - : Elsevier. - 1756-4646 .- 2214-9414. ; 112 Journal article (peer-reviewed)abstract Ulva polysaccharide (ULP) as main active ingredient of Ulva lactuca, has good pharmacological activity. This study further explored its anti-tumor mechanisms in hepatocellular carcinoma (HCC) with wet experiments and bioinformatic analyses. Results indicate that the differential proteins after ULP treatment are mainly enriched in immunomodulatory and complement pathways, among which the immune molecule CD5L and the key proteins of the membrane attack complex (MAC) are significantly upregulated, and the expression of MAC proteins are positively correlated with the expression of CD5L. Moreover, results from TIMER2.0 indicated that CD5L expression had positive correlation with CD8 + T cell infiltration in HCC, and single cell RNA-Seq analysis showed CD5L positive macrophage cells have higher expression of CFP, C1QA, C1QB, and C1QC, which are key molecular of complement cascades. Take together, the results suggest that ULP may exerts its anti-tumor activity by induces the expression of CD5L in HCC.
34.	Xu, Xiaoying, et al. (author) Metallic Wood through Deep-Cell-Wall Metallization : Synthesis and Applications 2024 In: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 16:17, s. 22433-22442 Journal article (peer-reviewed)abstract Metallic wood combines the unique structural benefits of wood and the properties of metals and is thus promising for applications ranging from heat transfer to electromagnetic shielding to energy conversion. However, achieving metallic wood with full use of wood structural benefits such as anisotropy and multiscale porosity is challenging. A key reason is the limited mass transfer in bulk wood where fibers have closed ends. In this work, programmed removal of cell-wall components (delignification and hemicellulose extraction) was introduced to improve the accessibility of cell walls and mass diffusion in wood. Subsequent low-temperature electroless Cu plating resulted in a uniform continuous Cu coating on the cell wall, and, furthermore, Cu nanoparticles (NPs) insertion into the wood cell wall. A novel Cu NPs-embedded multilayered cell-wall structure was created. The unique structure benefits compressible metal-composite foam, appealing for stress sensors, where the multilayered cell wall contributes to the compressibility and stability. The technology developed for wood metallization here could be transferred to other functionalizations aimed at reaching fine structure in bulk wood.
35.	Xu, Yuanyuan, et al. (author) How similar are the venation and cuticular characters of Glossopteris, Sagenopteris and Anthrophyopsis? 2023 In: Review of Palaeobotany and Palynology. - : Elsevier. - 0034-6667 .- 1879-0615. ; 316 Journal article (peer-reviewed)abstract Venation architectures and cuticular micromorphology of leaf fossils play important roles in higher-level taxonomic segregation, as these characters are broadly fixed within major plant clades. Three common fossil plant taxa are characterized by similar-shaped leaves or leaflets and anastomosing venation to such an extent that examples have commonly been assigned to the wrong taxon in past studies where fragmentary or ill-preserved material is available. We use standardized descriptions of vein cross-connection types and stomatal features to compare and contrast the venation patterns and stomatal architectures of these genera. Our reanalysis of the macro- and micromorphology of Glossopteris, Sagenopteris and Anthrophyopsis leaves reveals important differences that help segregate these taxa even on the basis of incomplete specimens. Anthrophyopsis has distinctive alignments of vein cross-connections in the outer lamina and paracytic stomata consistent with those of Bennettitales. Glossopteris has perigenous and monocyclic—normally stephanocytic to actinocytic—stomata commonly protected in pits or by overarching papillae. Sagenopteris has more consistently evanescent midribs and surficial anomocytic or stephanocytic stomata with weakly modified subsidiary cells. Considering the putatively close relationship of glossopterids (Glossopteris), Caytoniales (Sagenopteris) and Bennettitales (here encompassing Anthrophyopsis) resolved as members of the ‘glossophyte’ clade in some past phylogenetic studies, cuticular features suggest that these groups are not closely related. In addition, anastomosing venation, superficially similar to that of Glossopteris, Sagenopteris and Anthrophyopsis appears to have arisen independently in numerous other plant groups suggesting that this character has ecological or physiological benefits and is strongly prone to homoplasy.
36.	Yan, Yinglin, et al. (author) Waste Office Paper Derived Cellulose-Based Carbon Host in Freestanding Cathodes for Lithium-Sulfur Batteries 2022 In: ChemElectroChem. - : John Wiley & Sons. - 2196-0216. ; 9:11 Journal article (peer-reviewed)abstract Due to large sulfur (S) content and simple manufacturing techniques, free-standing cathodes for lithium-sulfur (Li−S) batteries are gaining a lot of attention recently. Waste office paper, which is consumed in large quantities annually, was used to make a free-standing paper-based carbon (FPC) substrate, which inherited fiber-like morphology. In addition, reduced graphene oxide (rGO) nanosheets modified FPC (rGO@FPC) host was also prepared by a vacuum filtration method. After S impregnation, the FPC/S and rGO@FPC/S free-standing cathodes were employed in Li−S batteries. The rGO@FPC/S free-standing cathode exhibited extremely competitive electrochemical performance, including a reversible discharge capacity of 315 mAh g−1 at 0.5 C after 500 cycles. This is due to the uniform S distribution, which boosts the utilization ratio, and the significant blocking action for polysulfide ions, which prevents the redox shuttle effect.
37.	Yao, Zehan, et al. (author) Magnetization-induced optical rectification and inverse spin Hall effect for interfacial terahertz generation in metallic heterostructures 2021 In: Physical Review B. - 2469-9950. ; 103:20 Journal article (peer-reviewed)abstract Metallic heterostructure as promising terahertz (THz) spintronic emitters has promoted the development of both spintronics and THz science. However, the underlying physics at the metallic interface, such as the nonlinear optical effect, remains unclear. Herein, we present interface magnetization induced THz generation from metallic heterostructure consisting of Heusler alloy CoFeMnSi (CFMS) and Pd thin films. THz generation is ascribed to 35% contribution from the magnetization-induced optical rectification (MOR) and 65% contribution from inverse spin Hall effect (ISHE) based on the pump polarization and sample azimuthal angle dependent measurement. Furthermore, the contribution ratio of the MOR decreases to 12% via lowering the CFMS grown temperature, which is due to the reduced crystalline quality and possible metal to semiconductor transformation in CFMS. Our results not only clarify MOR and ISHE in metallic heterostructure for the scientific field, but they also benefit THz source optimization for the technology field.
38.	Yi, Yuanyuan, 1981, et al. (author) Property rights, tenure security and forest investment incentives: evidence from China's Collective Forest Tenure Reform 2014 In: Environment and Development Economics. - 1355-770X .- 1469-4395. ; 19:1 Special issue, s. 48-73 Journal article (peer-reviewed)abstract This paper assesses how tenure reform in China’s collective forest sector affects Chinese farmer households’ perception of tenure security and propensity to invest in their forestland. A large database consisting of information from 3,180 households in eight provinces from south to north is used to explore factors correlated with stronger perceived tenure security and determinants of forest related investment. The study adds to the limited research testing whether there is endogenous causality between investment and tenure security in forestland and finds that investment was not undertaken to enhance tenure security. In addition, the data allows for differentiation between perceived tenure security and contracted use and transferability rights. Overall, stronger contracted rights were found to affect investment. China’s forest tenure reform – where individual households can manage forestland, empowered by legal certification and stronger contract rights – has thus enhanced tenure security and encouraged forest investment.
39.	Zhang, Wei, et al. (author) Organic Salts as p-Type Dopants for Efficient LiTFSI-Free Perovskite Solar Cells 2020 In: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 12:30, s. 33751-33758 Journal article (peer-reviewed)abstract Despite the ubiquity and importance of organic hole-transport materials in photovoltaic devices, their intrinsic low conductivity remains a drawback. Thus, chemical doping is an indispensable solution to this drawback and is essentially always required. The most widely used p-type dopant, FK209, is a cobalt coordination complex. By reducing Co(III) to Co(II), Spiro-OMeTAD becomes partially oxidized, and the film conductivity is initially increased. In order to further increase the conductivity, the hygroscopic co-dopant LiTFSI is typically needed. However, lithium salts are normally quite hygroscopic, and thus, water absorption has been suggested as a significant reason for perovskite degradation and therefore limited device stability. In this work, we report a LiTFSI-free doping process by applying organic salts in relatively high amounts. The film conductivity and morphology have been studied at different doping amounts. The resulting solar cell devices show comparable power conversion efficiencies to those based on conventional LiTFSI-doped Spiro-OMeTAD but show considerably better long-term device stability in an ambient atmosphere.
40.	Zhuang, Ting, et al. (author) SHARPIN stabilizes estrogen receptor a and promotes breast cancer cell proliferation 2017 In: Oncotarget. - : Impact Journals, LLC. - 1949-2553. ; 8:44, s. 77137-77151 Journal article (peer-reviewed)abstract Estrogen receptor a is expressed in the majority of breast cancers and promotes estrogen-dependent cancer progression. In our study, we identified the novel E3 ubiquitin ligase SHARPIN function to facilitate ERα signaling. SHARPIN is highly expressed in human breast cancer and correlates with ERα protein level by immunohistochemistry. SHARPIN expression level correlates with poor prognosis in ERα positive breast cancer patients. SHARPIN depletion based RNA-sequence data shows that ERα signaling is a potential SHARPIN target. SHARPIN depletion significantly decreases ERα protein level, ERα target genes expression and estrogen response element activity in breast cancer cells, while SHARPIN overexpression could reverse these effects. SHARPIN depletion significantly decreases estrogen stimulated cell proliferation in breast cancer cells, which effect could be further rescued by ERα overexpression. Further mechanistic study reveals that SHARPIN mainly localizes in the cytosol and interacts with ERα both in the cytosol and the nuclear. SHARPIN regulates ERα signaling through protein stability, not through gene expression. SHARPIN stabilizes ERα protein via prohibiting ERα protein poly-ubiquitination. Further study shows that SHARPIN could facilitate the mono-ubiquitinaiton of ERα at K302/303 sites and facilitate ERE luciferase activity. Together, our findings propose a novel ERα modulation mechanism in supporting breast cancer cell growth, in which SHARPIN could be one suitable target for development of novel therapy for ERα positive breast cancer.

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