| 1. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Li, Jian, et al.
(author)
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Assembly and succession of the phyllosphere microbiome and nutrient-cycling genes during plant community development in a glacier foreland
- 2024
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In: Environment International. - 0160-4120. ; 187
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Journal article (peer-reviewed)abstract
- The phyllosphere, particularly the leaf surface of plants, harbors a diverse range of microbiomes that play a vital role in the functioning of terrestrial ecosystems. However, our understanding of microbial successions and their impact on functional genes during plant community development is limited. In this study, considering core and satellite microbial taxa, we characterized the phyllosphere microbiome and functional genes in various microhabitats (i.e., leaf litter, moss and plant leaves) across the succession of a plant community in a low-altitude glacier foreland. Our findings indicate that phyllosphere microbiomes and associated ecosystem stability increase during the succession of the plant community. The abundance of core taxa increased with plant community succession and was primarily governed by deterministic processes. In contrast, satellite taxa abundance decreased during plant community succession and was mainly governed by stochastic processes. The abundance of microbial functional genes (such as C, N, and P hydrolysis and fixation) in plant leaves generally increased during the plant community succession. However, in leaf litter and moss leaves, only a subset of functional genes (e.g., C fixation and degradation, and P mineralization) showed a tendency to increase with plant community succession. Ultimately, the community of both core and satellite taxa collaboratively influenced the characteristics of phyllosphere nutrient-cycling genes, leading to the diverse profiles and fluctuating abundance of various functional genes during plant community succession. These findings offer valuable insights into the phyllosphere microbiome and plant–microbe interactions during plant community development, advancing our understanding of the succession and functional significance of the phyllosphere microbial community.
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| 3. |
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- 2019
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Journal article (peer-reviewed)
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| 4. |
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| 5. |
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| 6. |
- Harris, Ted D., et al.
(author)
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What makes a cyanobacterial bloom disappear? : A review of the abiotic and biotic cyanobacterial bloom loss factors
- 2024
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In: Harmful Algae. - : Elsevier. - 1568-9883 .- 1878-1470. ; 133
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Research review (peer-reviewed)abstract
- Cyanobacterial blooms present substantial challenges to managers and threaten ecological and public health. Although the majority of cyanobacterial bloom research and management focuses on factors that control bloom initiation, duration, toxicity, and geographical extent, relatively little research focuses on the role of loss processes in blooms and how these processes are regulated. Here, we define a loss process in terms of population dynamics as any process that removes cells from a population, thereby decelerating or reducing the development and extent of blooms. We review abiotic (e.g., hydraulic flushing and oxidative stress/UV light) and biotic factors (e.g., allelopathic compounds, infections, grazing, and resting cells/programmed cell death) known to govern bloom loss. We found that the dominant loss processes depend on several system specific factors including cyanobacterial genera -specific traits, in situ physicochemical conditions, and the microbial, phytoplankton, and consumer community composition. We also address loss processes in the context of bloom management and discuss perspectives and challenges in predicting how a changing climate may directly and indirectly affect loss processes on blooms. A deeper understanding of bloom loss processes and their underlying mechanisms may help to mitigate the negative consequences of cyanobacterial blooms and improve current management strategies.
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| 7. |
- Jin, Ying-Hui, et al.
(author)
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Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19 : An evidence-based clinical practice guideline (updated version)
- 2020
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In: Military Medical Research. - : Springer Science and Business Media LLC. - 2054-9369. ; 7:1
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Journal article (peer-reviewed)abstract
- The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of a rapidly spreading illness, coronavirus disease 2019 (COVID-19), affecting more than seventeen million people around the world. Diagnosis and treatment guidelines for clinicians caring for patients are needed. In the early stage, we have issued "A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version)"; now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline. We formed a working group of clinical experts and methodologists. The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas: chemoprophylaxis, diagnosis, treatments, and discharge management. We searched the literature for direct evidence on the management of COVID-19, and assessed its certainty generated recommendations using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach. Recommendations were either strong or weak, or in the form of ungraded consensus-based statement. Finally, we issued 34 statements. Among them, 6 were strong recommendations for, 14 were weak recommendations for, 3 were weak recommendations against and 11 were ungraded consensus-based statement. They covered topics of chemoprophylaxis (including agents and Traditional Chinese Medicine (TCM) agents), diagnosis (including clinical manifestations, reverse transcription-polymerase chain reaction (RT-PCR), respiratory tract specimens, IgM and IgG antibody tests, chest computed tomography, chest x-ray, and CT features of asymptomatic infections), treatments (including lopinavir-ritonavir, umifenovir, favipiravir, interferon, remdesivir, combination of antiviral drugs, hydroxychloroquine/chloroquine, interleukin-6 inhibitors, interleukin-1 inhibitors, glucocorticoid, qingfei paidu decoction, lianhua qingwen granules/capsules, convalescent plasma, lung transplantation, invasive or noninvasive ventilation, and extracorporeal membrane oxygenation (ECMO)), and discharge management (including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge). We also created two figures of these recommendations for the implementation purpose. We hope these recommendations can help support healthcare workers caring for COVID-19 patients.
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| 8. |
- Jin, Zhan-Kui, et al.
(author)
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Outcomes of total knee arthroplasty in the adult Kashin-Beck disease with severe osteoarthritis.
- 2019
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In: International Orthopaedics. - : Springer. - 0341-2695 .- 1432-5195. ; 43:2, s. 323-331
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Journal article (peer-reviewed)abstract
- PURPOSE: Kashin-Beck disease (KBD) is an endemic osteoarthropathy, and the severe knee pain and functional limitations were seriously affecting the quality of life in patients with end-stage KBD. We retrospectively evaluated the clinical outcomes and the quality of life in KBD patients with total knee arthroplasty (TKA).METHODS: A total of 22 subjects (25 knees) suffered KBD with severe knee pain and underwent primary TKA. Knee pain was measured by visual analogue scale (VAS), and the knee function was evaluated by Knee Society Clinical Rating System Score (KSS). KBD Quality of Life (KBDQOL) was used to evaluate the quality of life in KBD patients before and after TKA.RESULTS: There were no major complications after TKA. The levels of VAS score were obviously deceased in post-operation than that in pre-operation. The levels of KSS score were increased in one year after TKA compared with the pre-operative values, and it maintained a higher level on three years after TKA. The average KBDQOL score level of each domain in pre-operation and one and three years after TKA was increased accordingly. The average scores of physical function, activity limitation, support of society, mental health, and general health in one year after TKA were significantly higher than those in pre-operation.CONCLUSIONS: TKA can reduce knee pain, improve knee function, and improve the quality life in KBD patients. KBDQOL questionnaire may be a promising instrument for assessing the quality life in KBD patients.
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| 9. |
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| 10. |
- Qing, Jian, et al.
(author)
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Spacer Cation Alloying in Ruddlesden-Popper Perovskites for Efficient Red Light-Emitting Diodes with Precisely Tunable Wavelengths
- 2021
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In: Advanced Materials. - : Wiley-V C H Verlag GMBH. - 0935-9648 .- 1521-4095. ; 33:49
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Journal article (peer-reviewed)abstract
- Perovskite light-emitting diodes (PeLEDs) have recently shown significant progress with external quantum efficiencies (EQEs) exceeding 20%. However, PeLEDs with pure-red (620-660 nm) light emission, an essential part for full-color displays, remain a great challenge. Herein, a general approach of spacer cation alloying is employed in Ruddlesden-Popper perovskites (RPPs) for efficient red PeLEDs with precisely tunable wavelengths. By simply tuning the alloying ratio of dual spacer cations, the thickness distribution of quantum wells in the RPP films can be precisely modulated without deteriorating their charge-transport ability and energy funneling processes. Consequently, efficient PeLEDs with tunable emissions between pure red (626 nm) and deep red (671 nm) are achieved with peak EQEs up to 11.5%, representing the highest values among RPP-based pure-red PeLEDs. This work opens a new route for color tuning, which will spur future developments of pure-red or even pure-blue PeLEDs with high performance.
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| 11. |
- Reinl, Kaitlin L., et al.
(author)
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Cyanobacterial blooms in oligotrophic lakes : Shifting the high-nutrient paradigm
- 2021
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In: Freshwater Biology. - : John Wiley & Sons. - 0046-5070 .- 1365-2427. ; 66:9, s. 1846-1859
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Journal article (peer-reviewed)abstract
- Freshwater cyanobacterial blooms have become ubiquitous, posing major threats to ecological and public health. Decades of research have focused on understanding drivers of these blooms with a primary focus on eutrophic systems; however, cyanobacterial blooms also occur in oligotrophic systems, but have received far less attention, resulting in a gap in our understanding of cyanobacterial blooms overall. In this review, we explore evidence of cyanobacterial blooms in oligotrophic freshwater systems and provide explanations for those occurrences. We show that through their unique physiological adaptations, cyanobacteria are able to thrive under a wide range of environmental conditions, including low-nutrient waterbodies. We contend that to fully understand cyanobacterial blooms, and thereby mitigate and manage them, we must expand our inquiries to consider systems along the trophic gradient, and not solely focus on eutrophic systems, thus shifting the high-nutrient paradigm to a trophic-gradient paradigm.
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| 12. |
- Yuan, Qin, et al.
(author)
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A late Eocene palynological record from the Nangqian Basin, TibetanPlateau: Implications for stratigraphy and paleoclimate
- 2017
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In: Palaeoworld. - Nanjing : Elsevier. - 1871-174X .- 1875-5887. ; 26, s. 369-379
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Journal article (peer-reviewed)abstract
- With the uplifting, large-scale thrusting and striking of the Tibetan Plateau, several Paleogene intracontinental basins formed within the northernTibetan Plateau (TP). Stratigraphical and paleoenvironmental studies of the sedimentary successions within these basins are critical for understandingPaleogene climatological changes in Eurasia. The Nangqian Basin, one of such basins, formed in the Yushu area of the northeastern Tibetan Plateau.A set of lacustrine sediments, dominated by red clasolite, marlite, and gypsum, developed in the Yang Ala section in this basin. Paleontologicalrecords from the Nangqian Basin remain poorly known. Here, we investigate the palynological assemblages of one sedimentary succession at theYang Ala section that belongs to the Gongjue Formation, and their implications regarding the geological age and paleoclimate are discussed. Theresults reveal that the assemblages are dominated mainly by angiosperm pollen (tricolpates and tricolporate), includingNitrariadites (Pokrovskaja), Quercoidites, and Labitricolpites, followed by gymnosperm pollen taxa, such as Ephedripites and Taxodiaceaepollenites, and sparse pteridophytespores produced by ferns. A late Eocene age is inferred based on palynostratigraphy and comparison with other pollen assemblages in the TP. Arelatively dry climate with brief humid periods is indicated by the high abundance of xerophytic pollen taxa, such as Ephedripites and Nitrariadites,which are associated with broadleaved deciduous and evergreen plants. The characteristics of the pollen assemblages from the studied Yang Alasection are consistent with other Cenozoic palynofloras from the Mahalagou Formation in the Xining Basin and with those of the Yaxicuo Groupin the Hoh Xil Basin. These results provide an improved stratigraphical scheme for parts of the Cenozoic and enrich the current knowledge of thevegetation history of the northeastern Tibetan Plateau.
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| 13. |
- Aad, G., et al.
(author)
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- 2010
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| 14. |
- Aad, G., et al.
(author)
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- 2011
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| 15. |
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| 16. |
- Aad, G., et al.
(author)
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- 2010
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| 17. |
- Aad, G., et al.
(author)
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- 2010
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| 18. |
- Aad, G., et al.
(author)
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- 2010
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